CN107656084A - Detect composition and its chip of concentration, device, method and system in blood - Google Patents
Detect composition and its chip of concentration, device, method and system in blood Download PDFInfo
- Publication number
- CN107656084A CN107656084A CN201710895605.5A CN201710895605A CN107656084A CN 107656084 A CN107656084 A CN 107656084A CN 201710895605 A CN201710895605 A CN 201710895605A CN 107656084 A CN107656084 A CN 107656084A
- Authority
- CN
- China
- Prior art keywords
- blood
- detection
- module
- msub
- blood testing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides composition and its chip of concentration, device, method and system in a kind of detection blood, and the blood test device includes the blood testing chip and detection device of electrical connection；Blood testing chip includes multiple electrodes and accommodating sample to be tested and the reaction tank of multiple electrodes, and the working electrode upper surface in multiple electrodes has using modified membrane made of nanometer two-dimensional material.Blood testing chip of the present invention passes through modified membrane made of coated with nano two-dimensional material on the working electrode (s, it can realize to compositions such as the blood glucose in blood and uric acid while detect, different application scenarios is gone for by the design to electrode lay-out, shape, to realize simplicity, quick detection to blood；Blood test device can realize quick online detection to component contents such as the blood glucose in blood and uric acid.
Description
Technical field
The invention belongs to blood testing technical field, being related to one kind can be to composition such as Blood Glucose, uric acid and its dense
Spend the chip, device, method and system of quick detection.
Background technology
As living standard improves constantly, the concern for own health of people is constantly strengthened, while some chronic diseases
Also increasingly perplex people life, therefore can voluntarily the component content such as quick detection Blood Glucose, uric acid demand by
Gradually highlight.At present, the blood detector species of in the market is less, and can only typically realize the detection of blood glucose, for uric acid etc. its
He can not detect important component, and generally existing accuracy is poor simultaneously, operating process is complicated, testing cost is higher, detection
In place of the deficiencies of time is long.
The detection blood glucose and the method for uric acid reported at present mainly have High Performance Liquid Chromatographv, electrochemical process, enzyme process, chemistry
Luminescence method etc..In these methods, Direct Electrochemistry method has quick, and easy and sensitivity is high to be waited a little, while directly electricity
Chemical method is reacted without the participation of bioactive enzyme, and reaction is not influenceed by enzymatic activity size, but existing the type senses
Device generally existing uniformity and antijamming capability are poor, can not detect multiple compositions (such as uric acid and blood glucose) etc. in blood simultaneously
Problem.Therefore currently there is an urgent need to a kind of cost is cheap, detection detection means quick, easy to operate is to Blood Glucose and urine
The compositions such as acid carry out content analysis, and this promotes the sustainable development of socio-economy to have the further quality of life for improving people
Great meaning.
The content of the invention
The invention aims to solve the above problems, there is provided one kind can to compositions such as Blood Glucose, uric acid and
The blood testing chip of its concentration quick detection.
The above-mentioned purpose of the present invention is realized by solution below：
A kind of blood testing chip, including reaction tank and multiple electrodes, reaction tank house sample to be tested and the multiple electricity
Pole, working electrode is comprised at least in multiple electrodes, there is modified membrane working electrode upper surface, and modified membrane uses nanometer two-dimensional material system
Into；The nanometer two-dimensional material is bismuth film, perfluoro sulfonic acid membrane, gold nano grain, S, S '-di-2-ethylhexylphosphine oxide (N, N- diisobutyl two
Thiocarbamate), N, N, N ', the N '-tetrabutyl -3,6- dioxaoctanes two (sulphamide), (the 4- first of 5,10,15,20- tetra-
Phenyl) porphyrine cobalt (II), the 7,16- dibenzyl -1,4,10,13- of 1,10- dibenzyl -1,10- diaza -18- crown-s 6
At least one in the azo-cycle octadecanes of four oxygen -7,16- two, N, N '-dibenzyl -4,13- diaza -18- crown- 6- ethers and graphene
Kind.
Above-mentioned blood testing chip also includes substrate (1), and the multiple electrode forms conductive layer (2), conductive layer (2) and instead
Ying Chi (6) is arranged in substrate (1), and the multiple electrode is located in reaction tank (6).
In above-mentioned blood testing chip, the multiple number of poles is three, respectively working electrode (21), reference electrode
(22) and to electrode (23), the working electrode (21), reference electrode (22) and external-connected port is respectively equipped with to electrode (23)
(24)；Reference electrode (22) upper surface is covered with diaphragm (5), and the diaphragm (5) is provided with three layers, respectively silver layer, chlorine
Change the agarose film of silver layer and mixed chlorinated salt.
In above-mentioned blood testing chip, the working electrode (21) is circular electrode, to electrode (23) to working electrode
(21) bend；Or the working electrode (21) is arranged in a crossed manner in pectination with to electrode (23) and does not contact；Or the work
Electrode (21) is rectangular electrode, and electrode (23) is bent to working electrode (21).
The present invention also provide it is a kind of can be to the compositions such as Blood Glucose, uric acid and its blood of concentration quick online detection
Detection means, specific solution are as follows：
A kind of blood test device, including detection device (30) and the blood testing described in any one of Claims 1-4
Chip (10), the external-connected port (24) of blood testing chip (10) are electrically connected to the detection device (30)；The detection device
(30) apply voltage excitation signals to blood testing chip (10), and receive the electric signal of blood testing chip (10) feedback.
In above-mentioned blood test device, the detection device (30) includes embeded processor module (301), programmable electricity
Press output module (302), micro-current detection module (303) and detect port (304), programmable voltage output module (302) and
Micro-current detection module (303) is electrically connected respectively to embeded processor module (301), and respectively by detecting port (304)
Electrically connect the external-connected port (24) of blood testing chip (10)；The embeded processor module (301) drives programmable voltage
Output module (302) applies voltage drive letter by detecting port (304) to the external-connected port (24) of blood testing chip (10)
Number；The micro-current detection module (303) is by detecting external-connected port of port (304) reception from blood testing chip (10)
(24) current signal of feedback, and by current signal transfer to embeded processor module (301) analyzing and processing.
In above-mentioned blood test device, the embeded processor module (301) includes main control chip and memory cell, main
Control chip internal is implanted with autotest, and the autotest is used for detection pattern and detection parameters according to setting
Magnitude of voltage is obtained, programmable voltage output module (302) is driven by detecting port (304) to blood according to acquired magnitude of voltage
The external-connected port (24) of liquid detection chip (10) applies voltage excitation signals, and the telecommunications fed back according to blood testing chip (10)
Number molten appearance potential position and dissolution peak height angle value are calculated to determine the composition of sample to be tested and its concentration；Memory cell prestores
Given data parameter, including blood glucose dissolution peak characteristic value corresponding with the blood testing chip batch number, uric acid dissolution peak feature
Value and dissolution peak height angle value and blood glucose or the calibration curve of uric acid concentration.
In above-mentioned blood test device, the detection device (30) also includes precision resistance detection module (311), accurate electricity
Resistance detection module (311) is electrically connected to embeded processor module (301), and is electrically connected to blood by detecting port (304)
The external-connected port (24) of detection chip (10), the resistance signal for blood testing chip (10) to be fed back are transmitted to embedded place
Manage device module (301).
In above-mentioned blood test device, the detection device (30) is additionally provided with wireless communication module (310), described wireless
Communication module (310) and mobile terminal (309) radio communication, user input detection pattern and detection by mobile terminal (309)
Parameter；The wireless communication module (310) includes one kind in bluetooth module, Wifi modules, Zigbee module, 433MHz modules
It is or a variety of；
The mobile terminal (309) includes：
Response Code scan module (3091), Quick Response Code or other identification codes in scanning blood testing chip (10) to obtain
Take the id information of the blood testing chip；
Server lookup module (3092), receive the blood testing chip (10) that Response Code scan module (3091) obtains
Id information, and inquire about according to the id information details of the blood testing chip；
Wireless network module (3093), radio communication is carried out with the wireless communication module (310) of detection device (30)；
Test pattern selecting module (3094), obtains detection pattern and detection parameters are transmitted to wireless network module
(3093)；
Data memory module (3095), the relevant information that storage wireless network module (3093) obtains；
Pushing module (3096), information is obtained and to display circle of mobile terminal (309) from data memory module (3095)
Face is pushed for showing.
Above-mentioned blood test device also includes Keysheet module (307), and detection device (30) is obtained by Keysheet module (307)
The detection pattern and detection parameters of user's input.
Present invention also offers a kind of blood testing, this method is operated using above-mentioned blood test device, bag
Include following steps：
Step S701：Obtain the detection pattern and detection parameters of the blood test device set；
Step S702：Detection device (30) is according to detection pattern and detection parameters to the blood testing core added with sample to be tested
Piece (10) applies voltage excitation signals；
Step S703：Detection device (30), which receives the electric current that blood testing chip (10) feeds back according to voltage excitation signals, to be believed
Number；
Step S704：The current signal that detection device (30) feeds back to blood testing chip (10) is removed ambient noise
Processing, and calculates molten appearance potential position P and dissolution peak height angle value H, according to molten appearance potential position P determine sample to be tested into
Divide, the concentration of each composition in sample to be tested is determined according to dissolution peak height angle value H.
In above-mentioned blood testing, the voltage excitation signals can be the voltage signal of linear change, pulse signal
With the voltage signal of the voltage signal or square-wave signal and linear change Signal averaging of linear change Signal averaging.
In above-mentioned blood testing, in the step S704, detection device (30) passes through its embeded processor module
(301) molten appearance potential position and dissolution peak height angle value are calculated according to current signal, and determines to treat according to molten appearance potential position
The composition of test sample sheet and the concentration that each composition in sample to be tested is determined according to dissolution peak height angle value, comprise the following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position
P；
Step 2：It is pre- more than one in each selection in peak point P both sides one point A, B and A, 2 points of B tangent slope change
If threshold value, then A points are defined as left margin point, B points are defined as right margin point B；
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to following formula (1)：
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is dissolution peak electricity
Gesture position P coordinate value；
Step 4：The concentration of certain composition in sample to be tested, instant appearance height value are determined according to calculating dissolution peak height angle value H
H, which substitutes into dissolution peak height angle value and the calibration curve of concentration, can calculate the corresponding concentration of dissolution peak height angle value H；
Step 5：Molten appearance potential position P is judged whether in the range of characteristic values of certain blood constituent, if it is, sentencing
Blood constituent corresponding to fixed molten appearance potential position P, the then concentration for the composition obtained in step 4；The blood constituent is
Other compositions in blood glucose, uric acid or blood.
The present invention also provides a kind of blood detection system, is realized using solution below to Blood Glucose, uric acid etc.
Composition and its concentration quick online detection：
A kind of blood detection system, including：
Above-mentioned blood testing chip (10) and detection module, the detection module comprise at least：
Detection pattern and detection parameters acquiring unit (801), for obtaining the detection pattern and detection parameters of user's selection；
Voltage excitation signals applying unit (802), for according to detection pattern and detection parameters to added with sample to be tested
Blood testing chip (10) applies voltage excitation signals；
Current signal receiving unit (803), fed back according to voltage excitation signals for receiving blood testing chip (10)
Current signal；
Molten appearance potential position and dissolution peak height angle value computing unit (804), for calculating dissolution peak according to current signal
Potential position P and dissolution peak height angle value H；With
Composition and concentration determining unit (805), for determining the composition of sample to be tested, root according to molten appearance potential position P
The concentration of each composition of sample to be tested is determined according to dissolution peak height angle value H.
In above-mentioned blood detection system, the molten appearance potential position and dissolution peak height angle value computing unit (804) are specific
For performing following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position
P；
Step 2：It is pre- more than one in each selection in peak point P both sides one point A, B and A, 2 points of B tangent slope change
If threshold value, then A points are defined as left margin point, B points are defined as right margin point B；
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to below equation (1)：
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is dissolution peak electricity
Gesture position P coordinate value.
In above-mentioned blood detection system, the composition and concentration determining unit (805) are according to the dissolution peak electricity being calculated
Gesture position determines the composition of sample to be tested and the concentration of each composition of sample to be tested, detailed process is determined according to dissolution peak height angle value
Comprise the following steps：
Step A：The concentration of certain composition in sample to be tested, instant appearance height value H are determined according to calculating dissolution peak height angle value H
The corresponding concentration of dissolution peak height angle value H can be calculated by substituting into dissolution peak height angle value and the calibration curve of concentration.
Step B：Molten appearance potential position P is judged whether in the range of characteristic values of certain blood constituent, if it is, judging
Blood constituent corresponding to molten appearance potential position P, the then concentration for the composition obtained in step A；The blood constituent is blood
Other compositions in sugar, uric acid or blood.
The present invention has following features due to taking the above to design：Blood testing chip of the present invention passes through in working electrode
Modified membrane made of upper coated with nano two-dimensional material, the compositions such as the blood glucose in blood and uric acid can be detected simultaneously；It is logical
Cross the design to electrode lay-out, shape and go for the different application scenarios such as high accuracy or wide-range, to realize to blood
Simplicity, quick detection；Blood test device uses the automatic perform detection process of detection device, and easy to operate, detection speed is fast,
The quick online detection of the compositions such as Blood Glucose, uric acid and its concentration can be realized.
Brief description of the drawings
Fig. 1 is the structural representation of the embodiment one of blood testing chip of the present invention；
Fig. 2 is the structural representation of conductive layer in Fig. 1；
Fig. 3 is the structural representation of the embodiment two of blood testing chip of the present invention；
Fig. 4 is the structural representation of the embodiment three of blood testing chip of the present invention；
Fig. 5 is the process schematic diagram of blood testing chip structure of the present invention；
Fig. 6 is the embodiment A of blood test device of the present invention structured flowchart；
Fig. 7 is the embodiment B of blood test device of the present invention structured flowchart；
Fig. 8 is the topology example of mobile terminal；
Fig. 9 is the detection curve schematic diagram of blood test device of the present invention；
Figure 10 is the flow chart of blood testing of the present invention；
Figure 11 is the structural representation of blood detection system of the present invention.
Major Symbol：
10：Blood testing chip
1：Substrate；
2：Conductive layer, 21：Working electrode, 22：Participation electrode, 23：To electrode, 24：External-connected port；
3：Insulating barrier, 31：Connecting portion；
4：Modified membrane；
5：Diaphragm；
6：Reaction tank；
30：Detection device, 301：Embeded processor module, 302：Programmable voltage output module, 303：Micro-current is examined
Survey module, 304：Detect port；
305：Power module, 3051：Charging module, 3052：Multi-voltage power supply module, 3053：Battery；
306：Constant potential voltage module, 307：Keysheet module, 308：Display screen；
309：Mobile terminal, 3091：Response Code scan module, 3092：Server lookup module, 3093：Wireless network mould
Block, 3094：Test pattern selecting module, 3095：Data memory module, 3096：Pushing module；
310：Wireless communication module, 311：Precision resistance detection module.
Embodiment
For accuracy existing for current blood detector is poor, operating process is complicated, testing cost is higher, detection time
The deficiencies of long, and the uniformity and antijamming capability of electrochemistry class sensor generally existing it is poor, blood can not be detected simultaneously
In multiple composition (such as uric acid and blood glucose) the problems such as, the present invention provides that a kind of cost is cheap, detection blood quick, easy to operate
Liquid detection chip, device, method and system, apparatus of the present invention can detect multiple compositions and its concentration in blood simultaneously, and
Quick online detection can be realized to component contents such as the blood glucose in blood and uric acid.
Blood test device
Blood test device of the present invention includes blood testing chip 10 and detection device 30 (with reference to figure 6), blood testing core
Piece 10 is electrically connected to detection device 30, and the two is used cooperatively, and the device is realized in blood using electric potential scanning detection method
The component content on-line quick detection such as blood glucose and uric acid.As a kind of embodiment, blood test device of the present invention also includes one
Wireless communication module 310 (with reference to figure 7), the module carry out radio communication (with reference to figure 8) with a mobile terminal 309, are easy to user
Detection pattern (including quick detection pattern and accurate detection pattern) and corresponding detection parameters are set on mobile terminal 309
(for example, frequency and amplitude of the scanning voltage being applied on blood testing chip 10).
The structure of blood testing chip of the present invention is described in detail below.
Blood testing chip 10 of the present invention includes reaction tank and multiple electrodes, wherein, reaction tank is used to house sample to be tested
And multiple electrodes；Working electrode upper surface in multiple electrodes is provided with modified membrane, and modified membrane is using nanometer two-dimensional material (such as stone
The black new conductible two-dimension nano materials of alkene), susceptibility of this nanometer of two-dimensional material to compositions such as the blood glucose in blood, uric acid
Higher, therefore, blood testing chip of the present invention can realize the blood detected simultaneously in sample to be tested by the design of modified membrane
The compositions such as sugar, uric acid.Nanometer two-dimensional material is also referred to as two-dimension nano materials, is made up of several layer crystal bodies, there is special performance, Neng Gouzhi
Hold free electron ultrahigh speed flowing, electronics can quickly with pass through the material glibly.
Specifically, the material of the modified membrane on the working electrode may be selected from bismuth film, perfluoro sulfonic acid membrane, gold nano grain, S,
S '-di-2-ethylhexylphosphine oxide (N, N- diisobutyl dithiocarbamate), N, N, N ', the N '-tetrabutyl -3,6- dioxaoctanes two
(sulphamide), 5,10,15,20- tetra- (4- methoxyphenyls) porphyrine cobalt (II), 1,10- dibenzyl -1,10- diazas -18-
The azo-cycle octadecanes of 6 four oxygen -7,16- of 7,16- dibenzyl -1,4,10,13- of crown- two, N, N '-dibenzyl -4,13- diazas -18-
At least one of crown- 6- ethers and graphene.
In embodiment one shown in Fig. 1 and Fig. 2, the blood testing chip includes substrate 1, the conduction being arranged in substrate 1
Layer 2 and reaction tank 6 and the insulating barrier 3 being arranged on conductive layer 2, wherein, conductive layer 2 includes multiple electrodes and and electrode
The external-connected port to connect one to one, in the present embodiment electrode be provided with three, respectively working electrode 21, the and of reference electrode 22
To electrode 23, modified membrane 4 is coated with working electrode 21, the upper surface of reference electrode 22 is coated with diaphragm 5 to improve the reference
The stability of electrode potential in sample to be tested；Above three electrode is connected to external-connected port 24, and adjacent two external
The distance between port 24 is between 0.01~4mm, and the thickness of conductive layer 2 is between 10~1000nm, according to the need of processing technology
Will, conductive adhesive layer, such as layers of chrome or titanium layer can be increased between substrate 1 and conductive layer 2, thickness is between 5~50nm.Insulation
Layer 3 covers working electrodes 21, reference electrode 22, to electrode 23 and each the connecting portion 31 between external-connected port 24, insulating barrier 3 are right
The problems such as short circuit occurs for connecting portion 31 when the connecting portion 31 has protective effect to avoid instilling sample to be tested or operation；Reaction tank
6 is in the substrate 1 and adjacent with insulating barrier 3, working electrode 21, reference electrode 22 and electrode 23 is respectively positioned in reaction tank 6, work
Sample to be tested addition contacts in reaction tank 6 with each electrode when making.
Diaphragm 5 on reference electrode 22 is to protect reference electrode 22 to be independent of outside influences, so as to provide stabilizing potential,
In the embodiment, diaphragm 5 is provided with three layers, is respectively silver-colored (Ag) layer, silver chlorate (AgCl) layer and mixing certain concentration chlorination
The agarose film (such as it is 5% agarose powder mixed liquor to prepare 3mol/L KCl solution and mass concentration) of salt.
The blood testing chip structure of the embodiment three shown in embodiment two and Fig. 4 shown in Fig. 3 and above-described embodiment one
Structure it is essentially identical, difference is, the layouts of three electrodes and shape are different, Fig. 1 and the embodiment one shown in Fig. 2
In, working electrode 21 is circular electrode, and electrode 23 is bent to working electrode 21；In embodiment two shown in Fig. 3, working electrode
21 with being in that pectination is (not in contact with) arranged in a crossed manner to electrode 23；In embodiment three shown in Fig. 4, working electrode 21 is rectangle electricity
Pole, to electrode 23 to 21 L-shaped bending of working electrode.Electrode in above three embodiment is functionally consistent, in property
Different on energy, the electrode lay-out and shape of embodiment one are applied to the application scenarios of low precision wide-range, embodiment two
Electrode shape make it that its background current is smaller, and sensitivity is higher, suitable for the application scenarios of the narrow range of high accuracy, embodiment three
Electrode performance is between the electrode of embodiment one and embodiment two.In summary, the electrode work(of three of the above layout and shape
Energy is identical, performance is different, suitable for different application scenarios.
The structure of blood testing chip of the present invention is described all by taking three electrodes as an example for above three embodiment, but
The number of poles of the conductive layer 2 of the present invention is not limited to three.
Four steps (by taking three-electrode structure as an example) can be broadly divided into the process of chip structure of the present invention, referring to Fig. 5：
Step 1：Adhere to conductive layer in substrate
1) substrate 1 is cleaned
The substrate 1 of blood testing chip is the material for being easy to make flat surface, it is preferable that the substrate of blood testing chip
1 material is selected from glass, silicon, quartz, polymethyl methacrylate, polyethylene, polyvinyl chloride, polystyrene, polypropylene, poly- fourth
One kind in alkene, polyamide, polycarbonate, polytetrafluoroethylene (PTFE) and PET.To ensure that conductive layer 2 can adhere to
In in substrate 1, substrate 1 is ultrasonically treated and cleaned with oxygen gas plasma cleaning machine, ensure the cleaning on the surface of substrate 1.
2) processing of conductive layer 2
The material of conductive layer 2 is the inactive, conductive material such as gold, platinum, carbon, by sputtering, being deposited, silk-screen printing, the work such as plating
Skill machines.Conductive layer 2 has three-electrode structure, first has to make covering with three electrode shapes before conductive layer 2 is processed
Diaphragm plate, then operated for Different electrodes material.
If gold electrode, conductive layer 2 is one layer of golden film, can be followed the steps below：
(I), photoetching
In 1 even one layer of negtive photoresist of substrate, and toasted on baking oven or hot plate to increase photoresist and silicon chip adhesive capacity；
Mask plate is put to the relative position on the base 1, adjusting mask plate and substrate 1, is then placed on litho machine, adjusts litho machine
Power and time, photoetching is carried out to substrate 1；After photoetching is complete, mask plate is removed, substrate 1 is placed in developer solution and developed, then
Take out and dried up with nitrogen.
(II), plated film
Substrate 1 is put into magnetic control sputtering device or the evaporation vacuum coating equipment such as instrument, installs target, it is evacuated to 5 ×
10-4Below Pa, into plating mem stage, when thickness reaches setting thickness, plated film terminates.Base is taken out by standard of instruments operational procedure
Bottom 1, then by substrate 1 put in a suitable solvent, remove photoresist, drying i.e. obtain with golden film conductive layer substrate (referring to
Fig. 5 (a) width).Polylith substrate can be made in a substrate 1, after being completed by preceding method scribing can obtain single
Substrate.
If conductive carbon electrode, conductive layer 2 is conductive carbon layer, then carries out step (III)：
(III), silk-screen printing
Mask plate is put to the relative position on the base 1, adjusting mask plate and substrate 1, is then placed on high precision wire screen
On printing machine, silkscreen process is carried out using conductive carbon pastes, after the completion of remove drying after mask plate and obtain band conductive carbon layer
Substrate (referring to Fig. 5 (a) width).
Step 2：Electrode overlay film
It is included on reference electrode 22 plus diaphragm 5 and on working electrode 21 plus modified membrane 4.
Wherein, the procedure of processing of diaphragm 5 of reference electrode 22 is as follows：
(1) substrate is taken to be put into supersonic cleaning machine, it is right successively using cleaning agents such as acetone, isopropanol, certain density hydrochloric acid
It is cleaned by ultrasonic, and is cleaned between different cleaning agent cleaning processes with deionized water, after the completion of dry up, in working electrode and right
Electrode surface applies gluey isolated material, then waits isolated material solidification.
(2) certain density silver ammino solution is configured, and ensures the cleaning of container；
(3) substrate is put into silver ammino solution, reference electrode 22 is accessed into electrochemical workstation, voltage cycle is applied to substrate
A fixing turn is scanned, makes the upper surface depositing silver layers of reference electrode 22；
(4) after cleaning drying with water, substrate is put into certain density hydrochloric acid, reference electrode 22 is accessed into electrochemistry work
Stand, apply the voltage of certain time to substrate, silver chloride layer is covered on the depositing silver layers of the upper surface of reference electrode 22；
(5) 3mol/L KCl solution and the agarose powder mixed liquor that mass concentration is 5%, uniform stirring heating are prepared
Afterwards, it is coated in the reference electrode surface after step (4) is handled using sol evenning machine is even；
(6) using working electrode described in cutter and tweezers strip step (1) and the isolated material to electrode surface.
Processing to the upper table surface protective film 5 of reference electrode 22 is completed by step (1)-step (6), the diaphragm 5 by
In be outwards sequentially distributed silver layer, silver chloride layer and agarose film.
The procedure of processing of the modified membrane 4 of working electrode 21 is as follows：
Contain perfluoro sulfonic acid membrane (Nafion membrane), S, S '-di-2-ethylhexylphosphine oxide (isobutyl of N, N- bis- in the modified membrane 4 of working electrode
Base dithiocarbamate), N, N, N ', the N '-tetrabutyl -3,6- dioxaoctanes two (sulphamide), 5,10,15,20- tetra-
(4- methoxyphenyls) porphyrine cobalt (II), 1,10- dibenzyl -1,10- diaza -18- crown- 67,16- dibenzyl -1,4,10,
In the azo-cycle octadecanes of tetra- oxygen -7,16- of 13- two, N, N '-dibenzyl -4,13- diaza -18- crown- 6- ethers and graphene at least
One kind, its coating process are as follows：
1. compound concentration is 1%~10% suspension containing the material of target modified membrane 4；
2. substrate is placed on sol evenning machine, fix, start shooting and mix up rotating speed, after stabilization of speed, 10 are taken with liquid-transfering gun
Microlitre modified membrane suspension, drops to the upper surface of working electrode 21, is shut down after 20 seconds and removes substrate, and above step can be repeated several times
Suddenly；
3. setting oven temperature, substrate is placed in baking oven, modification membrane solvent is baked to and evaporates, taking out substrate is
Complete the overlay film to the surface of working electrode 21.
By step 2, the surface of working electrode 21 is covered with modified membrane 4 in the conductive layer on substrate, and the surface of reference electrode 22 is covered
There is diaphragm 5, referring to Fig. 5 (b) width.
Step 3：Paste insulating barrier
In working electrode 21, reference electrode 22, the position of connecting portion 31 between electrode 23 and respective external-connected port 24 is pasted
Attached insulating materials forms insulating barrier 3, referring to Fig. 5 (c) width.
Step 4：Reaction tank is installed
Reaction tank 6 is arranged on the lower section of insulating barrier 3 and surrounds working electrode 21, reference electrode 22 and to electrode 23, instead
Pond 6 and 1 closely sealed stickup of substrate are answered, referring to Fig. 5 (d) width.
Above-mentioned blood testing chip 10 can coordinate detection device 30 to use.In use, the reaction of blood testing chip 10
Sample to be tested (blood) is placed with pond 6, external-connected port 24 is electrically connected to the detection device 30, swept by detection device 30 according to current potential
Detection method is retouched, the concentration of the composition such as blood glucose or uric acid in sample to be tested is determined according to curve.
Detection device 30 for blood test device described below, in embodiment A as shown in Figure 6, including it is embedded
Processor module 301, programmable voltage output module 302, micro-current detection module 303, detection port 304 and power module
305, wherein：
Embeded processor module 301 is the brain of whole detection device, including main control chip and memory cell, master control core
Autotest is implanted with inside piece, the autotest passes through in existing linear ramp output program, square wave
The exploitation of the logical constraint conditions such as signal flow direction, execution sequence is merged on the basis of voltage output program, voltage detecting program etc. to complete,
Autotest is used to obtain magnitude of voltage according to the detection pattern and detection parameters of setting, is driven according to acquired magnitude of voltage
Programmable voltage output module 302 applies voltage drive by detecting port 304 to the external-connected port 24 of blood testing chip 10
Signal, and the electric signal fed back according to blood testing chip 10 calculates molten appearance potential position and dissolution peak height angle value to determine to treat
The composition and its concentration of test sample sheet；Memory cell is preset with given data parameter, including believes with the ID of blood testing chip (10)
Cease blood glucose characteristic value, uric acid characteristic value and the dissolution peak height angle value corresponding to (such as batch number) and the school of blood glucose or uric acid concentration
Directrix curve etc..
Programmable voltage output module 302 is electrically connected to embeded processor module 301, embeded processor module 301
Can be according to the detection pattern (including quick detection pattern and accurate detection pattern) and detection parameters of setting (for example, being applied to
The frequency and amplitude of scanning voltage on blood testing chip 10) obtain the magnitude of voltage for being input to blood testing chip 10, and root
Programmable voltage output module 302 is driven by detecting port 304 to the external of blood testing chip 10 according to the magnitude of voltage of acquisition
Port 24 applies voltage excitation signals, and voltage excitation signals can be the voltage signal or pulse signal of linear change
With the voltage signal of linear change Signal averaging, or the voltage signal of square-wave signal and linear change Signal averaging, voltage
The parameter of pumping signal is different according to different application scene；Power module 305 is that detection device 30 is powered.
Micro-current detection module 303 is electrically connected to embeded processor module 301, and by detecting port 304 and blood
The external-connected port 24 of detection chip 10 electrically connects, for receiving the current electrical signal drawn from external-connected port 24 and believing the electric current
Number transmit to embeded processor module 301 to analyze and process.
Embeded processor module 301 removes first after the current signal of the transmission of micro-current detection module 303 is received
Ambient noise, according to (Stripping Voltammetry is bent except the current signal after making an uproar and corresponding voltage excitation signals draw electric current-potential curve
Line), and dissolution peak is calculated according to the curve, wherein, Stripping Voltammetry curve is drawn out according to stripping voltammetry, i.e. blood
The working electrode 21 of detection chip 10 with to forming loop in solution of the electrode 23 in reaction tank 6, reference electrode 22 provides surely
Fixed reference potential, determinand is in programmable voltage output module 302 by detecting port 304 to the electricity of blood testing chip 10
Redox reaction occurs in working electrode 21 in the case of pole application voltage, and produces electric current, embeded processor module 301
Electric current-potential curve is drawn after receiving the electric current, so as to determine potential position according to the voltage-potential curve, calculate
Except the dissolution peak height angle value after after making an uproar, and the species of sample to be tested is determined according to molten appearance potential position, according to dissolution peak heights
Value determines the concentration of sample to be tested.
Composition and its concentration of the detection device 30 based on electric potential scanning detection method detection sample to be tested, a kind of mode are：
The embeded processor module 301 of detection device 30 removes ambient noise first after receiving current signal, then
Electric current-potential curve is drawn according to the current signal after denoising and corresponding voltage excitation signals, and it is molten according to curve calculating
Appearance potential position and dissolution peak height angle value, and then the composition of determination sample to be tested and its concentration are (here only with blood constituent blood
Exemplified by sugar and uric acid), reference picture 9, specifically include following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position
P。
Step 2：It is pre- more than one in each selection in peak point P both sides one point A, B and A, 2 points of B tangent slope change
If threshold value, then A points are defined as left margin point, B points are defined as right margin point B.
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to below equation (1)：
Wherein, (XA,YA) be left margin point A coordinate；(XB,YB) be right margin point B coordinate；(XP,YP) it is dissolution peak
Potential position P coordinate.
Step 4：The concentration of certain composition in sample to be tested, instant appearance height value are determined according to calculating dissolution peak height angle value H
(calibration curve is that existing be fitted according to multiple sample datas generates to the calibration curve of H substitution dissolution peak height angle value and concentration
Dissolution peak height angle value and concentration linear relationship curve) the corresponding concentration of dissolution peak height angle value H can be calculated.
Step 5：Judge whether (existing empirical value, is stored in advance in molten appearance potential position P in blood glucose range of characteristic values
In the memory cell of embeded processor module 301) in, if it is, judging blood constituent corresponding to molten appearance potential position P
For blood glucose, then obtained in step 4 for blood sugar concentration；Otherwise judge molten appearance potential position P whether in uric acid range of characteristic values
In (existing empirical value, be stored in advance in the memory cell of embeded processor module 301), if it is, judging dissolution peak
Blood constituent corresponding to the P of potential position is uric acid, then obtained in step 4 for uric acid concentration.
If electric current-the potential curve drawn there are multiple peak points, composition and concentration can be carried out one by one according to the method described above
Judge.Such as in the curve map shown in Fig. 9, it is 0.31V to have molten appearance potential corresponding to two peak point P and Q, P point, Q points pair
The molten appearance potential answered is 0.42V；And for blood glucose range of characteristic values typically between (0.3V-0.4V), uric acid characteristic value model
Enclose typically between (0.4V-0.5V), therefore be blood glucose constituents corresponding to peak point P, be uric acid composition corresponding to peak point Q.
Composition and its concentration of the detection device 30 based on electric potential scanning detection method detection sample to be tested, another way
For：
Detection device 30 also includes precision resistance detection module 311, and the precision resistance detection module 311 has multrirange certainly
Dynamic switching and high-precision feature；Precision resistance detection module 311 is electrically connected respectively to detect port 304 and embedded processing
Device module 301, the resistance signal for blood testing chip 10 to be fed back is transmitted to embeded processor module 301, embedded
Processor module 301 removes ambient noise first after receiving the resistance signal of the feedback of blood testing chip 10, then according to electricity
Resistance determines the concentration of sample to be tested relative to the rate of change of former resistance value, draws final testing result.
In embodiment B as shown in Figure 7, in order to ensure the reference electrode 22 of blood testing chip 10 and electrode 23 examined
Possesses identical potential during survey, detection device 30 is additionally provided with constant potential voltage module 306, the constant potential voltage module 306
Electrically connected respectively with programmable voltage output module 302 and detection port 304.
In this embodiment, detection device 30 also includes a Keysheet module 307, and detection device 30 can pass through Keysheet module
307 obtain the detection pattern and detection parameters of user's input, wherein, different test patterns correspond to different samples to be tested, such as
It can be the sample containing uric acid, or contain uric acid and glucose sample etc. simultaneously.
Detection device 30 is additionally provided with display screen 308, and the display screen can show the sample to be tested that detection device 30 obtains
Composition and its concentration, it can also show the electric current-potential curve drawn such as Fig. 9.
Power module 305 includes charging module 3051, multi-voltage power supply module 3052 and battery 3053, multi-voltage power supply mould
Block 3052 is electrically connected with battery 3053 and charging module 3051 respectively, and a power supply is provided with multi-voltage power supply module 3052 and is opened
Close；Charging module 3051 electrically connects with embeded processor module 301, be provided with charging module 3051 charging inlet to
Access external power supply is charged.
In embodiment shown in Fig. 7, detection device 30 is additionally provided with wireless communication module 310, the module and mobile terminal
309 carry out radio communication, and user can input detection pattern and corresponding detection parameters by mobile terminal 309.Radio communication mold
Block 310 can include the one or more in bluetooth module, Wifi modules, Zigbee module, 433MHz modules.
As shown in figure 8, mobile terminal 309 includes Response Code scan module 3091, server lookup module 3092, wireless network
Network module 3093, test pattern selecting module 3094, data memory module 3095 and pushing module 3096, wherein：
Response Code scan module 3091 scans Quick Response Code on blood testing chip 10 or other identification codes to obtain blood
The id information of detection chip, and by the details of the inquiry blood testing of server lookup module 3092 chip 10, this is detailed
The dissolution peak characteristic value that information includes every batch of chip (now the ID signals of blood testing chip correspond to its batch number) is (i.e. above-mentioned
The information such as blood glucose range of characteristic values, uric acid range of characteristic values), calibration curve (before dispatching from the factory test can obtain, every batch of chip all can be complete
Unanimously), calibration curve is the important parameter for calculating sample to be tested concentration.
Mobile terminal 309 carries out nothing by the wireless communication module 310 of the wireless network module 3093 and detection device 30
Line communicates, send the input of test pattern selecting module 3094 detection pattern and corresponding detection parameters to detection device 30, and
Sample to be tested composition and its concentration that detection device 30 is sent are received, and is stored by data memory module 3095.
Pushing module 3096 pushes the cost and its concentration information of sample to be tested to the display interface of mobile terminal 309, and
It has been shown that, so that user is checked.
In this embodiment, detection device 30 also includes shell, embeded processor module 301, display screen 308, can compile
Journey voltage output module 302, constant potential voltage module 306, micro-current detection module 303, power module 305, detection port
304th, wireless communication module 310 is mounted in the shell, and the various pieces in detection device are protected by the shell.
Detection device 30 obtains the detection pattern of user's selection and corresponding detection parameters, and according to detection pattern and parameter
Apply voltage excitation signals to blood testing chip 10；Sample to be tested, blood inspection are placed with the reaction tank 6 of blood testing chip 10
Survey chip 10 and generate current signal according to voltage excitation signals, and feed back to detection device 30；Detection device 30 is believed according to electric current
Number molten appearance potential position and dissolution peak height angle value are calculated, and the species of sample to be tested, root are determined according to molten appearance potential position
The concentration of sample to be tested is determined according to dissolution peak height angle value.
Blood test device of the present invention, device uniformity is good, and test multiplicity is high, and compact, is easy to carry, simultaneously
On-line quick detection is realized to component contents such as blood glucose and uric acid by electric potential scanning detection method.
Blood testing
The present invention also provides a kind of blood testing, is operated using above-mentioned blood test device, as shown in Figure 10,
The blood testing comprises the following steps：
Step S701：Obtain the detection pattern and detection parameters of the blood test device set；
Step S702：Detection device 30 is according to detection pattern and detection parameters to the blood testing chip added with sample to be tested
10 apply voltage excitation signals；
Step S703：Detection device 30 receives the current signal that blood testing chip 10 feeds back according to voltage excitation signals；
Step S704：The current signal that detection device 30 feeds back to blood testing chip 10 is removed at ambient noise
Reason, and molten appearance potential position and dissolution peak height angle value are calculated, the composition of sample to be tested, root are determined according to molten appearance potential position
The concentration of each composition in sample to be tested is determined according to dissolution peak height angle value.
Wherein, in step S704, detection device 30 is calculated molten by its embeded processor module 301 according to current signal
Appearance potential position and dissolution peak height angle value, and the composition of sample to be tested is determined and according to dissolution according to molten appearance potential position
Peak height angle value determines that the process of the concentration of each composition in sample to be tested comprises the following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position
P。
Step 2：It is pre- more than one in each selection in peak point P both sides one point A, B and A, 2 points of B tangent slope change
If threshold value, then A points are defined as left margin point, B points are defined as right margin point B.
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to below equation (1)：
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is dissolution peak electricity
Gesture position P coordinate value.
Step 4：The concentration of certain composition in sample to be tested, instant appearance height value are determined according to calculating dissolution peak height angle value H
(calibration curve is that existing be fitted according to multiple sample datas generates to the calibration curve of H substitution dissolution peak height angle value and concentration
Dissolution peak height angle value and concentration linear relationship curve) the corresponding concentration of dissolution peak height angle value H can be calculated.
Step 5：Whether judge molten appearance potential position P (by taking the blood glucose in blood sample to be measured and uric acid composition as an example)
In blood glucose range of characteristic values, if it is, judge that blood constituent corresponding to molten appearance potential position P is blood glucose, then step 4
In obtain for blood sugar concentration；Otherwise molten appearance potential position P is judged whether in uric acid range of characteristic values, if it is, sentencing
Blood constituent corresponding to fixed molten appearance potential position P is uric acid, then obtained in step 4 for uric acid concentration.
If electric current-the potential curve drawn there are multiple peak points, composition and concentration can be carried out one by one according to the method described above
Judge.Such as in the curve map shown in Fig. 9, it is 0.31V to have molten appearance potential corresponding to two peak point P and Q, P point, Q points pair
The molten appearance potential answered is 0.42V；And for blood glucose range of characteristic values typically between (0.3V-0.4V), uric acid characteristic value model
Enclose typically between (0.4V-0.5V), therefore be blood glucose constituents corresponding to peak point P, be uric acid composition corresponding to peak point Q.
Peak point P peak point current is highly 23.1 μ A, 12.5 μ A of peak point current height corresponding to peak point Q, therefore substitutes into empirical value
Formula is calculated and can obtained, blood sugar concentration 5.6mmol/L, uric acid concentration 0.178mmol/L, the blood glucose and uric acid detected
The actual concentrations value of concentration value and the blood testing sample is basically identical.
Blood detection system
The present invention also provides a kind of detecting system, and as shown in figure 11, the detecting system mainly includes blood testing chip 10
And include with 10 matching used detection module of blood testing chip, the detection module：
Detection pattern and detection parameters acquiring unit 801, voltage excitation signals applying unit 802, current signal receive single
Member 803, molten appearance potential position and dissolution peak height angle value computing unit 804, composition and concentration determining unit 805, wherein：
Detection pattern and detection parameters acquiring unit 801 are used for the detection pattern and detection parameters for obtaining user's selection；Electricity
Pumping signal applying unit 802 is pressed to be used for according to detection pattern and detection parameters to the blood testing chip 10 added with sample to be tested
Apply voltage excitation signals；Current signal receiving unit 803 is anti-according to voltage excitation signals for receiving blood testing chip 10
The current signal of feedback；Molten appearance potential position and dissolution peak height angle value computing unit 804 are used to calculate dissolution according to current signal
Peak potential position and dissolution peak height angle value；Composition and concentration determining unit 805 are to be measured for being determined according to molten appearance potential position
The composition of sample, the concentration of each composition of sample to be tested is determined according to dissolution peak height angle value.
Molten appearance potential position and dissolution peak height angle value computing unit 804 are specifically used for performing following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position
P。
Step 2：It is pre- more than one in each selection in peak point P both sides one point A, B and A, 2 points of B tangent slope change
If threshold value, then A points are defined as left margin point, B points are defined as right margin point B.
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to below equation (1)：
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is dissolution peak electricity
Gesture position P coordinate value.
Composition and concentration determining unit 805 can determine the composition of sample to be tested according to the molten appearance potential position being calculated
And the concentration of each composition of sample to be tested is determined according to dissolution peak height angle value, here using the sample to be tested comprising blood glucose and uric acid as
Example, detailed process comprise the following steps：
Step A：The concentration of certain composition in sample to be tested, instant appearance height value H are determined according to calculating dissolution peak height angle value H
(calibration curve is that existing be fitted according to multiple sample datas generates to the calibration curve of substitution dissolution peak height angle value and concentration
The linear relationship curve of dissolution peak height angle value and concentration) the corresponding concentration of dissolution peak height angle value H can be calculated.
Step B：Molten appearance potential position P is judged whether in blood glucose range of characteristic values, if it is, judging dissolution peak electricity
Blood constituent corresponding to the P of gesture position is blood glucose, then obtained in step A for blood sugar concentration；Otherwise molten appearance potential position P is judged
Whether in uric acid range of characteristic values, if it is, judging that blood constituent corresponding to molten appearance potential position P is uric acid, then walk
Obtained in rapid A for uric acid concentration.
Blood test device of the present invention uses modern sensor technique, and micron/nano process technology is completed, device uniformity
Good, test multiplicity is high, and compact, is easy to carry, and online quick inspection can be realized to component contents such as blood glucose and uric acid
Survey.
It will be appreciated by those skilled in the art that these embodiments or embodiment are merely to illustrate the present invention without limiting this
The scope of invention, the various equivalent variations and modification made to the present invention belong to the disclosure of invention.
Claims (16)
1. a kind of blood testing chip, it is characterised in that including reaction tank and multiple electrodes, reaction tank houses sample to be tested and institute
Multiple electrodes are stated, working electrode is comprised at least in multiple electrodes, there is modified membrane working electrode upper surface, and modified membrane uses nanometer two
Dimension material is made；The nanometer two-dimensional material is bismuth film, perfluoro sulfonic acid membrane, gold nano grain, S, S '-di-2-ethylhexylphosphine oxide (N, N- bis-
Isobutyl group dithiocarbamate), N, N, N ', the N '-tetrabutyl -3,6- dioxaoctanes two (sulphamide), 5,10,15,20-
Four (4- methoxyphenyls) porphyrine cobalts (II), 7,16- dibenzyl -1,4 of 1,10- dibenzyl -1,10- diaza -18- crown-s 6,
In the azo-cycle octadecanes of tetra- oxygen -7,16- of 10,13- two, N, N '-dibenzyl -4,13- diaza -18- crown- 6- ethers and graphene extremely
Few one kind.
2. blood testing chip according to claim 1, it is characterised in that also including substrate (1), the multiple electrode shape
Into conductive layer (2), conductive layer (2) and reaction tank (6) are arranged in substrate (1), and the multiple electrode is located in reaction tank (6).
3. blood testing chip according to claim 1 or 2, it is characterised in that the multiple number of poles is three, point
Wei not working electrode (21), reference electrode (22) and to electrode (23), the working electrode (21), reference electrode (22) and to electricity
Pole (23) is respectively equipped with external-connected port (24)；Reference electrode (22) upper surface is covered with diaphragm (5), the diaphragm (5)
Provided with three layers, the respectively agarose film of silver layer, silver chloride layer and mixed chlorinated salt.
4. blood testing chip according to claim 3, it is characterised in that the working electrode (21) is circular electrode,
Electrode (23) is bent to working electrode (21)；Or the working electrode (21) with to electrode (23) in pectination it is arranged in a crossed manner and
Do not contact；Or the working electrode (21) is rectangular electrode, electrode (23) is bent to working electrode (21).
5. a kind of blood test device, it is characterised in that including described in detection device (30) and any one of Claims 1-4
Blood testing chip (10), the external-connected port (24) of blood testing chip (10) is electrically connected to the detection device (30)；Institute
State detection device (30) and apply voltage excitation signals to blood testing chip (10), and receive blood testing chip (10) feedback
Electric signal.
6. blood test device according to claim 5, it is characterised in that the detection device (30) includes embedded place
Device module (301), programmable voltage output module (302), micro-current detection module (303) and detection port (304) are managed, can be compiled
Journey voltage output module (302) and micro-current detection module (303) are electrically connected respectively to embeded processor module (301), and
The external-connected port (24) of blood testing chip (10) is electrically connected by detecting port (304) respectively；The embeded processor mould
Block (301) driving programmable voltage output module (302) is by detecting external connection end of the port (304) to blood testing chip (10)
Mouth (24) applies voltage excitation signals；The micro-current detection module (303) is received from blood testing by detecting port (304)
The current signal of external-connected port (24) feedback of chip (10), and by current signal transfer to embeded processor module (301)
To analyze and process.
7. blood test device according to claim 6, it is characterised in that embeded processor module (301) bag
Main control chip and memory cell are included, autotest is implanted with inside main control chip, the autotest is used for basis
The detection pattern and detection parameters of setting obtain magnitude of voltage, and programmable voltage output module is driven according to acquired magnitude of voltage
(302) by detect external-connected port (24) from port (304) to blood testing chip (10) apply voltage excitation signals, and according to
The electric signal of blood testing chip (10) feedback calculates molten appearance potential position and dissolution peak height angle value to determine sample to be tested
Composition and its concentration；Memory cell prestores given data parameter, including blood glucose corresponding with the blood testing chip batch number
Dissolution peak characteristic value, uric acid dissolution peak characteristic value and dissolution peak height angle value and blood glucose or the calibration curve of uric acid concentration.
8. the blood test device according to claim 6 or 7, it is characterised in that the detection device (30) also includes essence
Cipher telegram resistance detection module (311), precision resistance detection module (311) is electrically connected to embeded processor module (301), and passes through
Detection port (304) is electrically connected to the external-connected port (24) of blood testing chip (10), for blood testing chip (10) is anti-
The resistance signal of feedback is transmitted to embeded processor module (301).
9. according to the blood test device described in any one of claim 5 to 8, it is characterised in that the detection device (30) is also
Wireless communication module (310) is provided with, the wireless communication module (310) and mobile terminal (309) radio communication, user pass through
Mobile terminal (309) inputs detection pattern and detection parameters；The wireless communication module (310) includes bluetooth module, Wifi moulds
One or more in block, Zigbee module, 433MHz modules；
The mobile terminal (309) includes：
Response Code scan module (3091), Quick Response Code or other identification codes in scanning blood testing chip (10) so that obtain should
The id information of blood testing chip；
Server lookup module (3092), receive the ID letters for the blood testing chip (10) that Response Code scan module (3091) obtains
Cease, and the details of the blood testing chip are inquired about according to the id information；
Wireless network module (3093), radio communication is carried out with the wireless communication module (310) of detection device (30)；
Test pattern selecting module (3094), obtains detection pattern and detection parameters are transmitted to wireless network module (3093)；
Data memory module (3095), the relevant information that storage wireless network module (3093) obtains；
Pushing module (3096), the display interface from data memory module (3095) acquisition information and to mobile terminal (309) push away
Send for showing.
10. according to the blood test device described in any one of claim 5 to 8, it is characterised in that also including Keysheet module
(307), detection device (30) obtains the detection pattern and detection parameters of user's input by Keysheet module (307).
11. a kind of blood testing, being operated using the blood test device described in any one of claim 5 to 10, wrap
Include following steps：
Step S701：Obtain the detection pattern and detection parameters of the blood test device set；
Step S702：Detection device (30) is according to detection pattern and detection parameters to the blood testing chip added with sample to be tested
(10) voltage excitation signals are applied；
Step S703：Detection device (30) receives the current signal that blood testing chip (10) feeds back according to voltage excitation signals；
Step S704：The current signal that detection device (30) feeds back to blood testing chip (10) is removed at ambient noise
Reason, and calculates molten appearance potential position P and dissolution peak height angle value H, according to molten appearance potential position P determine sample to be tested into
Divide, the concentration of each composition in sample to be tested is determined according to dissolution peak height angle value H.
12. blood testing according to claim 11, it is characterised in that the voltage excitation signals can be linear
The voltage signal of change, the voltage signal of pulse signal and linear change Signal averaging or square-wave signal and linear change signal are folded
The voltage signal added.
13. the blood testing according to claim 11 or 12, it is characterised in that in the step S704, detection is set
Standby (30) calculate molten appearance potential position and dissolution peak heights by its embeded processor module (301) according to current signal
Value, and the composition of sample to be tested is determined according to molten appearance potential position and determined according to dissolution peak height angle value each in sample to be tested
The concentration of composition, comprises the following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position P；
Step 2：Point A, B and an A, 2 points of B tangent slope change are respectively chosen in peak point P both sides more than a default threshold
Value, then be defined as left margin point by A points, B points be defined as into right margin point B；
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to following formula (1)：
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is molten appearance potential position
Put P coordinate value；
Step 4：The concentration of certain composition in sample to be tested, instant appearance height value H generations are determined according to calculating dissolution peak height angle value H
The corresponding concentration of dissolution peak height angle value H can be calculated by entering dissolution peak height angle value and the calibration curve of concentration；
Step 5：Molten appearance potential position P is judged whether in the range of characteristic values of certain blood constituent, if it is, judging molten
Blood constituent corresponding to the P of appearance potential position, the then concentration for the composition obtained in step 4；The blood constituent is blood
Other compositions in sugar, uric acid or blood.
A kind of 14. blood detection system, it is characterised in that including：
Blood testing chip (10) and detection module, the detection module described in any one of Claims 1-4 at least wrap
Include：
Detection pattern and detection parameters acquiring unit (801), for obtaining the detection pattern and detection parameters of user's selection；
Voltage excitation signals applying unit (802), for according to detection pattern and detection parameters to the blood added with sample to be tested
Detection chip (10) applies voltage excitation signals；
Current signal receiving unit (803), the electric current fed back for receiving blood testing chip (10) according to voltage excitation signals
Signal；
Molten appearance potential position and dissolution peak height angle value computing unit (804), for calculating molten appearance potential according to current signal
Position P and dissolution peak height angle value H；With
Composition and concentration determining unit (805), for determining the composition of sample to be tested according to molten appearance potential position P, according to molten
Appearance height value H determines the concentration of each composition of sample to be tested.
15. blood detection system according to claim 14, it is characterised in that the molten appearance potential position and dissolution peak
Height value computing unit (804) is specifically used for performing following steps：
Step 1：Peak point P is determined according to current signal, peak point P abscissa is defined as molten appearance potential position P；
Step 2：Point A, B and an A, 2 points of B tangent slope change are respectively chosen in peak point P both sides more than a default threshold
Value, then be defined as left margin point by A points, B points be defined as into right margin point B；
Step 3：According to left margin point A and right margin point B, dissolution peak height angle value H is calculated according to below equation (1)：
<mrow>
<mi>H</mi>
<mo>=</mo>
<mfrac>
<mrow>
<msub>
<mi>Y</mi>
<mi>P</mi>
</msub>
<msub>
<mi>X</mi>
<mi>B</mi>
</msub>
<mo>+</mo>
<msub>
<mi>Y</mi>
<mi>A</mi>
</msub>
<msub>
<mi>X</mi>
<mi>P</mi>
</msub>
<mo>-</mo>
<msub>
<mi>Y</mi>
<mi>P</mi>
</msub>
<msub>
<mi>X</mi>
<mi>A</mi>
</msub>
<mo>-</mo>
<msub>
<mi>Y</mi>
<mi>A</mi>
</msub>
<msub>
<mi>X</mi>
<mi>B</mi>
</msub>
<mo>-</mo>
<msub>
<mi>Y</mi>
<mi>B</mi>
</msub>
<msub>
<mi>X</mi>
<mi>P</mi>
</msub>
<mo>-</mo>
<msub>
<mi>Y</mi>
<mi>B</mi>
</msub>
<msub>
<mi>X</mi>
<mi>A</mi>
</msub>
</mrow>
<mrow>
<msub>
<mi>X</mi>
<mi>B</mi>
</msub>
<mo>-</mo>
<msub>
<mi>X</mi>
<mi>A</mi>
</msub>
</mrow>
</mfrac>
<mo>-</mo>
<mo>-</mo>
<mo>-</mo>
<mrow>
<mo>(</mo>
<mn>1</mn>
<mo>)</mo>
</mrow>
</mrow>
Wherein, XA,YAIt is left margin point A coordinate value；XB,YBIt is right margin point B coordinate value；XP,YPIt is molten appearance potential position
Put P coordinate value.
16. the blood detection system according to claims 14 or 15, it is characterised in that the composition and concentration determining unit
(805) composition of sample to be tested is determined according to the molten appearance potential position being calculated and determines to treat according to dissolution peak height angle value
The concentration of each composition of test sample sheet, detailed process comprise the following steps：
Step A：The concentration of certain composition in sample to be tested is determined according to calculating dissolution peak height angle value H, instant appearance height value H is substituted into
Dissolution peak height angle value and the calibration curve of concentration can calculate the corresponding concentration of dissolution peak height angle value H.
Step B：Molten appearance potential position P is judged whether in the range of characteristic values of certain blood constituent, if it is, judging dissolution
Blood constituent corresponding to the P of peak potential position, the then concentration for the composition obtained in step A；The blood constituent is blood glucose, urine
Other compositions in acid or blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710895605.5A CN107656084A (en) | 2017-09-28 | 2017-09-28 | Detect composition and its chip of concentration, device, method and system in blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710895605.5A CN107656084A (en) | 2017-09-28 | 2017-09-28 | Detect composition and its chip of concentration, device, method and system in blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107656084A true CN107656084A (en) | 2018-02-02 |
Family
ID=61116539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710895605.5A Withdrawn CN107656084A (en) | 2017-09-28 | 2017-09-28 | Detect composition and its chip of concentration, device, method and system in blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107656084A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210088468A1 (en) * | 2019-09-20 | 2021-03-25 | Avx Corporation | Somatic Cell-Based Electrical Biosensor |
| CN117481645A (en) * | 2023-12-11 | 2024-02-02 | 重庆医科大学绍兴柯桥医学检验技术研究中心 | Multi-index electrode for dynamically monitoring blood sugar, blood ketone and pH simultaneously |
-
2017
- 2017-09-28 CN CN201710895605.5A patent/CN107656084A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210088468A1 (en) * | 2019-09-20 | 2021-03-25 | Avx Corporation | Somatic Cell-Based Electrical Biosensor |
| CN117481645A (en) * | 2023-12-11 | 2024-02-02 | 重庆医科大学绍兴柯桥医学检验技术研究中心 | Multi-index electrode for dynamically monitoring blood sugar, blood ketone and pH simultaneously |
| CN117481645B (en) * | 2023-12-11 | 2024-07-09 | 重庆医科大学绍兴柯桥医学检验技术研究中心 | Multi-index electrode for dynamically monitoring blood sugar, blood ketone and pH simultaneously |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ji et al. | Smartphone-based cyclic voltammetry system with graphene modified screen printed electrodes for glucose detection | |
| US20170363572A1 (en) | Contaminant detection device and method | |
| US9823213B2 (en) | Electrochemical test strip, measurement system and method for determining sample content in the reactive region of the electrochemical test strip | |
| CN106198676B (en) | A kind of solid contact potassium ion-selective electrode and its preparation and application | |
| CN105353009B (en) | A kind of screen printing electrode and its processing method based on Graphene conductive ink | |
| CN112638255B (en) | Electrochemical sensor comprising a plurality of working electrodes and a common reference electrode | |
| CN106370714A (en) | Mobile terminal-based cyclic voltammetry biochemical detection device and method | |
| CN107656084A (en) | Detect composition and its chip of concentration, device, method and system in blood | |
| EP3844498A1 (en) | Electrochemical sensor including multiple work electrodes and common reference electrode | |
| CN107957440A (en) | Plane ammine selective sensing electrode and its preparation method | |
| WO2013143357A1 (en) | Automatic coding device, biosensor with same and manufacturing method therefor | |
| CN215297225U (en) | Novel electrochemical immunodetection system for coronavirus 2019-nCoV | |
| CN105004781A (en) | Dopamine detecting method based on paper-base electrochemistry device | |
| CN109298048A (en) | A kind of copper-based sensor chip and preparation method thereof and detection method | |
| CN112415063A (en) | Heavy metal detector | |
| CN207408418U (en) | A kind of blood testing chip | |
| Chen et al. | Fabrication and characterization of an innovative integrated solid-state microelectrode | |
| CN214097250U (en) | Heavy metal detector | |
| Kwon et al. | Direction of oxygen evolution reaction electrocatalyst evaluation for an anion exchange membrane CO 2 electrolyzer | |
| CN107064116B (en) | A kind of electrochemical luminescence biochemical detection system and method based on mobile phone USB-OTG interface | |
| CN109856202A (en) | It is a kind of measure seawater total alkalinity paper base Copper diethlydithiocarbamate and its application | |
| Bao et al. | Multichannel electrochemical workstation-based data collection combined with machine learning for online analysis of tyrosine | |
| CN108445059A (en) | POCT type COD detection system based on graphene oxide-nano nickel particle modified planar electrode and detection method thereof | |
| CN113514527B (en) | Ion detection method | |
| CN109799274A (en) | A kind of preparation and test method of conducting polymer membrane material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180202 |