CN107648432A - A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity - Google Patents
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity Download PDFInfo
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Abstract
The invention belongs to cultural technique field, and in particular to a kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, it is characterised in that：It is prepared in parts by weight including following raw material：80 parts of frankincense 40 part, 50 parts of caulis piperis futokadsurae 30 part, 50 parts of kelp 30 part, 40 parts of the Radix Astragali 30 part, 50 parts of the fleece-flower root 40 part, 30 parts of Chinese cassia tree 20 part, 35 parts of aloe 20 part, 40 parts of hawthorn 30 part, 30 parts of Semen Cuscutae 15 part, 15 parts of caraway seed 10 part, 20 parts of dark plum 10 part, 20 parts of jujube 10 part, windproof 5 parts 15 parts, 25 parts of radix glycyrrhizae 10 part.Advantage：After compounding, there is the effect that good palatability, food calling are whetted the appetite, and the immunity of black bottom soft-shelled turtle can be significantly improved, reduce illness rate.
Description
【Technical field】
The invention belongs to cultural technique field, and in particular to a kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity.
【Background technology】
Pingnan County ink bottom soft-shelled turtle, it is one of the high-quality freshwater fish in Guangxi, (agricultural product are geographical to be marked Chinese geography famous special product
Will).Originate in Guangxi Pingnan County Yu Jiang basins.From in the early 1990s, rivers fish wild seed is collected by some peasant households of Guangxi Pingnan County
Propagated artificially, then carry out artificial propagation success.The kind has good dressed fish height, meat, delicious flavour, nutriture value
It is worth the features such as high, is well received by consumers, wide market.Beginning at the beginning of 21 century, it is vast that guiding is focused in Guangxi Pingnan County
Soft-shelled turtle agriculture cultivates to cultivate to famous-particular-excellent kind from traditional category to be changed, and in December, 2005, fishery cultivating branch in Pingnan County set up, branch
Scattered raiser is concentrated in the form of member system, is managed collectively, in planning and designing, cultural technique, market information etc.
Service is provided for member.268 hectares of Terrapin raising area in 2006, breed variety is based on black bottom soft-shelled turtle, black bottom soft-shelled turtle cultured area
Reach 138 hectares, 280 tons of annual production.
But because mankind's activity is more and more, living environment, water quality are also increasingly severeer, black bottom soft-shelled turtle from young soft-shelled turtle cultivate into
Motility rate is more and more lower, in order to ensure the survival rate of young soft-shelled turtle, it is necessary to open up one can from young soft-shelled turtle in itself constitutionally improve antibacterial resist
Scorching ability, therefore, it is extremely urgent to find a kind of medicament that can be improved black bottom soft-shelled turtle immunity and have no side effect safely.
【The content of the invention】
The goal of the invention of the present invention is：It is an object of the invention to provide a kind of Chinese traditional medicine composition for improving black bottom soft-shelled turtle immunity
Thing, after compounding, there is the effect that good palatability, food calling are whetted the appetite, and the immunity of black bottom soft-shelled turtle can be significantly improved, drop
Low illness rate.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
40 parts -80 parts of frankincense, 30 parts -50 parts of caulis piperis futokadsurae, 30 parts -50 parts of kelp, 30 parts -40 parts of the Radix Astragali, 40 parts -50 parts of the fleece-flower root, meat
20 parts -30 parts of osmanthus, 20 parts -35 parts of aloe, 30 parts -40 parts of hawthorn, 15 parts -30 parts of Semen Cuscutae, 10 parts -15 parts of caraway seed, dark plum
10 parts -20 parts, 10 parts -20 parts of jujube, windproof 5 parts -15 parts, 10 parts -25 parts of radix glycyrrhizae.
Preferably, it is prepared in parts by weight including following raw material：50 parts -70 parts of frankincense, 35 part -45 of caulis piperis futokadsurae
Part, 35 parts -40 parts of kelp, 33 parts -38 parts of the Radix Astragali, 42 parts -48 parts of the fleece-flower root, 23 parts -27 parts of Chinese cassia tree, 25 parts -30 parts of aloe,
It is 32 parts -37 parts of hawthorn, 20 parts -27 parts of Semen Cuscutae, 11 parts -14 parts of caraway seed, 12 parts -18 parts of dark plum, 12 parts -18 parts of jujube, anti-
8 parts -12 parts of wind, 15 parts -20 parts of radix glycyrrhizae.
It is further preferred that it is prepared in parts by weight including following raw material：60 parts of frankincense, 40 parts of caulis piperis futokadsurae, elder brother
37 parts of cloth, 35 parts of the Radix Astragali, 45 parts of the fleece-flower root, 25 parts of Chinese cassia tree, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, 12 parts of caraway seed,
14 parts of dark plum, 15 parts of jujube, windproof 10 parts, 18 parts of radix glycyrrhizae.
It is described to be made by above-mentioned obtained Chinese medicine composition plus the auxiliary material commonly used in pharmacy, the agent of the medicament
Type is powder, pill or granule.
A kind of application for the Chinese medicine composition for improving black bottom soft-shelled turtle immunity, will improve the Chinese medicine composition of black bottom soft-shelled turtle immunity
Applied to preparing in black bottom trionyx sinensis feed additive.
Further instruction, frankincense are that water-bath adds at 30 DEG C by 100g frankincenses addition 200ml 75wt% ethanol solutions
Hot Soxhlet refluxing extraction 1-2 hours, go filtrate.
Further instruction, caulis piperis futokadsurae are that the 100g caulis piperis futokadsurae stems dried are pulverized and sieved by micronizer
0.2-0.5 microns are selected, the 90wt% ethanol solutions for adding 200ml are extracted, and recycling design obtains alcohol-extracted extract, will
Medicinal extract obtains the ethyl acetate extract of caulis piperis futokadsurae after being extracted with ethyl acetate, stand-by.
Further instruction, kelp are by after cold water immersion 30-40 minutes of the kelp using 2 times of volumes, rinse 3 kelps
Afterwards, the water heating for adding 3-5 times of volume decocts 30-40 minutes, filters to take filtrate, continues to decoct mistake after 30-40 minutes by the dregs of a decoction
Leaching filter residue, merge filtrate twice, after ultra micro membrane filtration, spray drying obtains powdered extract, stand-by.
Further instruction, the Radix Astragali be after the Radix Astragali is crushed according to volume ratio be 1:2, which add 80wt% ethanol solutions, is carried out
10-15 minutes are extracted, using microwave radiation exaraction, filtrate is filtered to take, filter residue is repeated the above steps 2-3 times, merging filtrate is
It can obtain Radix Astragali extractive solution.
Further instruction, the fleece-flower root are to add 5-10 times of water after 100g fleece-flower root root block is cut into slices to be decocted
Filtrate is filtered to take after 30 minutes, it is stand-by.
Further instruction, Chinese cassia tree are that 100g Chinese cassia tree crushed into 100 mesh sieves, are subsequently added into 5-10 times of material quantity
85wt% ethanol solutions carry out extracting 10-15 minutes, are extracted using ultrasonic assistant, filter to take filtrate.
Further instruction, aloe are that the 85wt% ethanol solutions of 5-10 times of addition after 100g fresh aloe is smashed to pieces enter
Row extraction 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate, it is stand-by.
Further instruction, hawthorn are the 85wt% ethanol solutions of 5-10 times of addition after 100g dry Fructus Crataegi is crushed
Carry out extracting 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate, powder is obtained after vacuum drying, is then used
Chloroform is extracted, take chloroform layer be concentrated under reduced pressure dry after obtain haw thorn extract, it is stand-by.
Further instruction, Semen Cuscutae are that the 85wt% ethanol solutions that 100g Semen Cuscutae tendril is added to 3 times of volumes carry
1-2 hours are taken, filter to take filtrate, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain extracting solution of south dodder seed.
Further instruction, caraway seed are the 85wt% ethanol solutions that 3 times of volumes are added after 100g caraway seed is crushed
1-2 hours are extracted, filter to take filtrate, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain caraway seed extract solution.
Further instruction, dark plum are that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry dark plum is crushed are carried out
Extraction
10-15 minutes, using microwave radiation exaraction, filtrate is filtered to take, powder is obtained after vacuum drying, then using chloroform
Extracted, take chloroform layer be concentrated under reduced pressure dry after obtain smoked plum extractive, it is stand-by.
Further instruction, jujube are that 100g jujube crushed 100 mesh sieves, are subsequently added into 3-5 times of 85wt% of material quantity
Ethanol solution carries out extracting 10-15 minutes, is extracted using ultrasonic assistant, filters to take filtrate.
Further instruction, windproof is that the 85wt% ethanol solutions that 100g windproof herb is added to 3 times of volumes extract 1-2
Hour, filtrate is filtered to take, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain windproof extract solution.
Further instruction, radix glycyrrhizae are small fire decoctions after the water immersion 10-15 minutes that 100g dry herb is added into 10 times
40-50 minutes can be filtrated to get licorice extract, stand-by.
The drug effect of above-mentioned Chinese medicine is as follows：
Frankincense is pungent, bitter, temperature.The thoughts of returning home, liver, the spleen channel.Containing boswellic acid, mastic hydrocarbon, frankincense hypo acid, masticinic acid etc..Separately
Oil containing performance, predominantly firpene；Relieved pain with blood-activating and qi-promoting, detumescence and promoting granulation.For chest impediment and cardialgia, gastral cavity pain, dysmenorrhoea warp
Close, postpartum stasis, abdominal mass stomachache, arthralgia pain due to rheumatism, muscle arteries and veins contraction, traumatic injury, carbuncle swells sore.
Caulis piperis futokadsurae Chinese medicine name.For Piperaceae plant wind rattan Piper kadsura (Choisy) 0hwi drying rattan.Summer,
Qiu Erji is tapped, and is removed root, leaf, is dried.Acrid flavour, hardship, slightly warm in nature.Return liver warp.Wind-damp dispelling, degrading the channel, stopping numbness pain.For wind
Cold-dampness numbness, podomere pain, muscle arteries and veins contraction, joint stuffiness.
Kelp (Ecklonia kurome) is also known as " black dish ", " goose slaps dish ", " five palm dishes " etc..Phaeophyceae, Alariaceae.Spore
Daughter is large-scale, brown, keratin, high 30-100cm, leaflet piece, shank, holdfast, holdfast rhizoid shape.The V shape point of rhizoid two
Branch, shank cylindrical shape, nearly blade part is gradually flat, and blade both sides pinniform or coverts shape branch, middle part is slightly thick, intercalary growth, long 1-
12cm, thick 3-7mm, rough sawn tingia edge.Zoospore is born in blade two sides.Have and do not wait the alternation of generations significantly.It is grown on temperate zone
In ocean.Zhejiang Province, China, ALONG COASTAL FUJIAN are distributed.Nature and flavor are trembled with fear, salty.Return liver, stomach, kidney channel.There are softening and resolving hard mass, detumescencing diuresis, profit
The work(of lower dissolving phlegm, clinically for goitre, lymphonodi cervicales is swollen, bronchitis, pulmonary tuberculosis, cough and cataract of old people
Deng also trying out in treating cancer.
The Radix Astragali, also known as continuous stilbene.Nature and flavor：It is sweet, tepor.Channel tropism：Return lung, spleen, liver and kidney channel.Perennial herb, high 50-100
Centimetre.Main root is plump, wooden, normal branch, canescence.Stem is upright, top multi-branched, there is thin rib, by white pubescence.It is perennial
Draft is high 50-100 centimetres.Originate in the ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang.The medicinal of the Radix Astragali has more than 2000 years so far
History, it has enhancing body's immunity, liver protection, diuresis, anti-aging, resisting stress, decompression and wide antibacterial action.
But table is real evil to be contained, and retention of dampness due to stagnation of QI, dyspepsia is stagnated, ulcer from the beginning of or heat toxin such as still contains at real example, and the hyperactivity of yang due to yin deficiency person after bursting,
Clothes must be prohibited.
The fleece-flower root (scientific name：Fallopia multiflora (Thunb.) Harald.), also known as spend more knotweed, purple black rattan, night
Hand over rattan etc..It is that the polygonaceae knotweed race fleece-flower root belongs to perennial winding liana, root tuber is plump, oblong, dark brown.Raw mountain valley
Shrubbery, hillside sylvan life, ditch curb gap.Produce Southern Shaanxi, SOUTH OF GANSU, East China, Central China, south China, Sichuan, Yunnan and Guizhou.It is bitter
It is sweet puckery, tepor.Sweet, temperature is nontoxic.Its root tuber is used as medicine, and can calm the nerves, be blood-nourishing, active, and removing toxic substances (preventing malaria), disappear carbuncle；RADIX POLYGONI MULTIFLORI PREPARATA can
Help essence and blood, black beard and hair, strengthening the bones and muscles, filling liver kidney, it is your common detail medicinal material.
Chinese cassia tree (Classification system：Cinnamomum cassia Presl), also known as beautiful osmanthus, male osmanthus, cajaput, great Gui, peppery osmanthus,
Safety tree, cassia, it is the dry bark of canella Chinese cassia tree.Bark fragrance, spices can be made, taste is with originating from Sri Lanka
The cassia bark of Chinese cassia tree is similar, but pepperyyer, delicious not as good as cassia bark, and thick compared with cassia bark.It is pungent, sweet, heat.Return kidney, spleen, the heart, Liver Channel.Mend fire
It is supporing yang, eliminating cold to stop pain, warming meridian.For the insufficiency of the kidney yang, positive fistula Gong Leng, the cold abdomen of gastral cavity abdomen, arthritis pain in the back, cold hernia stomachache, tremble with fear solidifying
Blood stasis, closed dysmenorrhea, chest impediment and cardialgia.
Aloe (scientific name：Aloe vera) Aloe is Liliaceae perennial evergreen herbaceous plant, leafage is raw, big and fertile
Thickness, in seat shape or stem top is born in, there is sharp tooth thorn at the normal lanceolar of leaf or the short width of leaf, edge.Inflorescence be umbrella shape, total shape, spike,
Cone etc., color is in red, yellow or tool redness of the skin or complexion spot, six, petal, six pieces of gynoecium.The more joints of perianth base portion are into tubular.Nature and flavor：
Hardship, tremble with fear.Removing heat from the liver, defaecation.For：Constipation, infantile malnutrition, infantile convulsion；Wet tinea is controlled outside.
Hawthorn, Chinese medicine name.For rosaceous plant large-fruited Chinese hawthorn Crataegus pin, natiida
Bge.var.majorN.E.Br. or hawthorn Crataegus pin.nati fida Bge. dry mature fruit.Autumn fruit
Harvest, cut into slices when real ripe, dry.It is sour, sweet, tepor.Returns spleen, stomach, Liver Channel.Food digesting stomach fortifying, scattered stasis, change turbid lipid-loweringing.With
It is high in meat stagnation, gastral cavilty turgor, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, confidant sting, chest impediment and cardialgia, hernia pain
Pionemia.Charred FRUCTUS CRATAEGI promoting digestion effect enhancing.It is not well for meat stagnation, rush down dysentery.
Semen Cuscutae (scientific name：Cuscuta chinensis Lam.), alias abdicates true, beans parasitism, beans King of Hell, yellow silk, yellow silk
Rattan, reticulate millettia, spun gold rattan etc..Annual parasitic draft.Stem is wound, and yellow is very thin, no leaf.Inflorescence side is given birth to, few to spend or spend more
Fasciation is into small umbrella shape or small glomerule；Bract and squamella are small, flakey；Bennet is slightly sturdy；Calyx cup-shaped, below middle part
Joint, sliver triangular shape；Corolla white, kettle shape；Stamen the raw micro- lower place of corolla lobe sinus；Scale Long Circle；The nearly ball of ovary
Shape, style 2.Capsule is spherical, is almost all the corolla harbored and is surrounded.Seed 2-49, it is filbert, it is avette, 1 millimeter is about, table
Face is coarse.It is sweet, warm, return kidney,liver,spleen to pass through, there is the effect of nourishing liver and kidney, astringent sperm contracting urine, antiabortive, improving eyesight, antidiarrheal, begin to carry《God
Agriculture book on Chinese herbal medicine warp》, it is listed in top grade.Sugariness typically has a tonic effect, sweet pungent tepor, reports gas and neutralizes, both can yang-tonifying, again can benefit
The moon, have it is warm but not dry, mend without it is stagnant the characteristics of.Controlling nocturnal emission with astringent drugs is antiabortive and gonadotropic Effect.The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution,
Interior residence Kidney-Yin and Yuanyang, is the root of human body reproductive development, the motive power of internal organs functional activity.
A kind of fruit of wild plant of caraway seed, autumn smoothes out with the fingers come after dry and wear into powder just into wormwood artemisia face.It is special that wormwood artemisia seed has
Smell, wormwood artemisia face has viscosity after meeting water, and wormwood artemisia seed, which has, promotees digestion, stomach invigorating, heat-clearing and other effects.
Dark plum Chinese medicine name.It is sour, puckery, it is mild-natured.Return liver, spleen, lung, large intestine channel.For rose department plant plum Prunus
Mume (Sieb.) Sieb.etZuce. drying almost ripe fruit.There is cultivation China various regions, with the Yangtze river basin with
Southern each province is most.With astringing the lung, puckery intestines, promote the production of body fluid, the effect of claming ascaris.It is usually used in chronic cough of deficiency lung, protracted dysentery and diarrhea, abnormal heat is quenched one's thirst, ascarid
Vomiting of fainting is suffered from abdominal pain.
Jujube, also known as jujube, belong to Angiospermae, Dicotyledoneae, Rhamnales, Rhamnaceae, the plant of zizyphus.Its
Vitamin content is very high, there is the good reputation of " natural complex ball ", has nourishment for vitality, the effect of enriching blood.It is sweet, temperature.Returns spleen,
Stomach.Tonifying middle-Jiao and Qi, nourishing blood and tranquilization, relax the property of medicine.For weakness of the spleen and the stomach, anorexia and loose stool, blood deficiency chlorosis, women's hysteria.
Windproof Chinese medicine name.For labiate Indian Epimeredi Herb Anisomeles indica (L.) O.Ktze herb.It is distributed in
Jiangxi, Zhejiang, Fujian, Taiwan, Hunan, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan and Tibet.With wind-dispelling, dehumidify, removing toxic substances
The effect of.It is usually used in body heat of catching a cold, vomits, stomachache, arthralgia and myalgia, sore, eczema, haemorrhoids.Acrid flavour, hardship, it is warm-natured.Return bladder,
Liver and kidney channel.
Radix glycyrrhizae (scientific name：Glycyrrhiza uralensis Fisch) alias：State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet tea root
Son.Pulse family, Glycyrrhiza perennial herb, root is sturdy with root-like stock, is a kind of help Chinese herbal medicine.To a kind of human body medicine well,
Medicinal part is root and rhizome, and Medicinal Materials Characters root is in cylinder, long 25~100 centimetres, 0.6~3.5 centimetre of diameter.Crust pine
Tightly differ, surface rufous or taupe brown.For rhizome in cylinder, there is bud scar on surface, has marrow in the middle part of section.Gas is micro-, sweet and special
Very.Major function is clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc..Happiness is dark moist, the low dry climate of the long temperature of sunshine.Radix glycyrrhizae is more
It is grown in arid, semiarid desert steppe, desert edge and loess hill area.Root and root-like stock hyoscine.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Chinese medicine principle of the present invention according to monarch, frankincense, caulis piperis futokadsurae, kelp, the Radix Astragali, fleece-flower root collective effect are had
It is monarch drug in a prescription altogether to have the effects such as myogenic, removing toxic substances, channels and collaterals, protect liver, antibacterial, and Chinese cassia tree, aloe, hawthorn, Semen Cuscutae collective effect have
Promote blood circulation dissolving stasis, help digestion, the effect of improving eyesight altogether be as ministerial drug, caraway seed, dark plum, jujube, windproof collective effect have stomach invigorating,
Food calling, the effect of clearing damp are that radix glycyrrhizae is clearing heat and detoxicating as adjutant altogether, and neutralizing the property of medicine and being used as makes medicine；14 taste medicine collective effects, phase
It is auxiliary to coordinate, existing cold and cool product in side, also there is the medicine of warm nature, using medicines of both cold and hot natures simultaneously, existing antibacterial, anti-inflammatory, anti-oxidant medicine, also have
Wind, the product of QI invigorating, mutually assistant system are dissipated, are cooperated, the prescription compatibility is proper, and all medicines play constitutional effect altogether, and treat
Significant effect, it can be achieved to treat both principal and secondary aspect of disease, it is securely and reliably, without any side effects.
【Embodiment】
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair
The embodiment of the present invention is described in detail.Elaborate many details in order to abundant in the following description
Understand the present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art
Similar improvement can be done in the case of without prejudice to intension of the present invention, therefore the present invention is not by following public specific implementation
Limitation.
The preparation of raw material：
Frankincense be by 100g frankincenses add 200ml 75wt% ethanol solutions at 30 DEG C heating water bath Soxhlet refluxing extraction
1-2 hours, go filtrate.
Caulis piperis futokadsurae is by micronizer pulverize and sieve by the caulis piperis futokadsurae stem that 100g is dried selecting 0.2-0.5 micro-
Rice, the 90wt% ethanol solutions for adding 200ml are extracted, and recycling design obtains alcohol-extracted extract, by medicinal extract ethyl acetate
The ethyl acetate extract of caulis piperis futokadsurae is obtained after extraction, it is stand-by.
Kelp is by after cold water immersion 30-40 minutes of the kelp using 2 times of volumes, after rinsing 3 kelps, adds 3-5 times
The water heating of volume decocts 30-40 minutes, filters to take filtrate, and the dregs of a decoction are continued to filter to take filter residue after decocting 30-40 minutes, closed
And filtrate twice, after ultra micro membrane filtration, spray drying obtains powdered extract, stand-by.
The Radix Astragali be after the Radix Astragali is crushed according to volume ratio be 1:2 addition 80wt% ethanol solutions carry out extracting 10-15 minutes,
Using microwave radiation exaraction, filtrate is filtered to take, filter residue is repeated the above steps 2-3 times, merging filtrate can obtain astragalus extraction
Liquid.
The fleece-flower root is to add 5-10 times of water after 100g fleece-flower root root block is cut into slices filter after decocting 30 minutes
Filtrate is taken, it is stand-by.
Chinese cassia tree is that 100g Chinese cassia tree crushed into 100 mesh sieves, is subsequently added into 5-10 times of 85wt% ethanol solution of material quantity and enters
Row extraction 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate.
Aloe is that the 85wt% ethanol solutions of 5-10 times of addition after 100g fresh aloe is smashed to pieces carry out extracting 10-15 points
Clock, extracted using ultrasonic assistant, filter to take filtrate, it is stand-by.
Hawthorn is that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry Fructus Crataegi is crushed carry out extraction 10-15
Minute, extracted using ultrasonic assistant, filter to take filtrate, powder is obtained after vacuum drying, is then extracted using chloroform,
Take chloroform layer be concentrated under reduced pressure dry after obtain haw thorn extract, it is stand-by.
Semen Cuscutae is that the 85wt% ethanol solutions that 100g Semen Cuscutae tendril is added to 3 times of volumes extract 1-2 hours, filtering
Filtrate is taken, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain extracting solution of south dodder seed.
Caraway seed is that the 85wt% ethanol solutions that 3 times of volumes are added after 100g caraway seed is crushed extract 1-2 hours, mistake
Leaching filtrate, filter residue repeat to extract, and merge the filtrate of 3 times, you can obtain caraway seed extract solution.
Dark plum is that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry dark plum is crushed carry out extracting 10-15 points
Clock, using microwave radiation exaraction, filtrate is filtered to take, powder is obtained after vacuum drying, is then extracted using chloroform, takes chlorine
Imitative layer be concentrated under reduced pressure dry after obtain smoked plum extractive, it is stand-by.
Jujube is that 100g jujube crushed 100 mesh sieves, is subsequently added into 3-5 times of 85wt% ethanol solution of material quantity and is carried
10-15 minutes are taken, are extracted using ultrasonic assistant, filter to take filtrate.
Windproof is that the 85wt% ethanol solutions that 100g windproof herb is added to 3 times of volumes extract 1-2 hours, filters to take filter
Liquid, filter residue repeat to extract, and merge the filtrate of 3 times, you can obtain windproof extract solution.
Radix glycyrrhizae is small fire decoction 40-50 minutes after the water immersion 10-15 minutes that 100g dry herb is added into 10 times
Licorice extract is filtrated to get, it is stand-by.
Embodiment 1：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
40 parts of frankincense, 30 parts of caulis piperis futokadsurae, 30 parts of kelp, 30 parts of the Radix Astragali, 40 parts of the fleece-flower root, 20 parts of Chinese cassia tree, 20 parts of aloe, 30 parts of hawthorn,
15 parts of Semen Cuscutae, 10 parts of caraway seed, 10 parts of dark plum, 10 parts of jujube, windproof 5 parts, 10 parts of radix glycyrrhizae.
Embodiment 2：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
80 parts of frankincense, 50 parts of caulis piperis futokadsurae, 50 parts of kelp, 40 parts of the Radix Astragali, 50 parts of the fleece-flower root, 30 parts of Chinese cassia tree, 35 parts of aloe, 40 parts of hawthorn,
30 parts of Semen Cuscutae, 15 parts of caraway seed, 20 parts of dark plum, 20 parts of jujube, windproof 15 parts, 25 parts of radix glycyrrhizae.
Embodiment 3：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
It is prepared in parts by weight including following raw material：50 parts of frankincense, 35 parts of caulis piperis futokadsurae, 35 parts of kelp, 33 parts of the Radix Astragali, what
42 parts of the tuber of multiflower knotweed, 23 parts of Chinese cassia tree, 25 parts of aloe, 32 parts of hawthorn, 20 parts of Semen Cuscutae, 11 parts of caraway seed, 12 parts of dark plum, 12 parts of jujube,
Windproof 8 parts, 15 parts of radix glycyrrhizae.
Embodiment 4：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
It is prepared in parts by weight including following raw material：70 parts of frankincense, 45 parts of caulis piperis futokadsurae, 40 parts of kelp, 38 parts of the Radix Astragali, what
48 parts of the tuber of multiflower knotweed, 27 parts of Chinese cassia tree, 30 parts of aloe, 37 parts of hawthorn, 27 parts of Semen Cuscutae, 14 parts of caraway seed, 18 parts of dark plum, 18 parts of jujube,
Windproof 12 parts, 20 parts of radix glycyrrhizae.
Embodiment 5：
A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity, is prepared including following raw material in parts by weight：
It is prepared in parts by weight including following raw material：60 parts of frankincense, 40 parts of caulis piperis futokadsurae, 37 parts of kelp, 35 parts of the Radix Astragali, what
45 parts of the tuber of multiflower knotweed, 25 parts of Chinese cassia tree, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, 12 parts of caraway seed, 14 parts of dark plum, 15 parts of jujube,
Windproof 10 parts, 18 parts of radix glycyrrhizae.
Embodiment 1 is prepared into powder A groups, embodiment 2-3 is prepared into B, C group pill, embodiment 4-5 is prepared into D,
E groups granule, 5 Chinese medicines be separately added into feed carry out feeding black bottom soft-shelled turtle,
Black bottom trionyx sinensis feed composition percetage by weight % is fed in the test group of table 1
1.1 test sites, condition：
Selection health, the basically identical childhood ink bottom soft-shelled turtle 600 of quality, mass of foundation is 265.5 ± 0.08g, at random
It is divided into 6 groups, respectively feeding test group 1-6 feed, is to black bottom soft-shelled turtle feeding 5 days, the feeding amount of daily every black bottom soft-shelled turtle continuously
0.5-0.8kg, daily feeding 3 times.
The 30% of morning feeding feed, feeding 40% in afternoon, evening feeding 30%.
It is that density is 20/m that the pond of raising, which can reach density,2, temperature is 28 ± 3 DEG C, pH 7.0-7.2, dissolved oxygen
More than 7mg/L, pond uses circulation.
1.2 feed result detection：
5 black bottom soft-shelled turtles are randomly choosed, carry out venous blood collection, 3500r/min is centrifuged 6 minutes, takes serum in 5ml centrifuge tubes
It is standby；Lysozyme (LSZ), catalase (CAT), superoxide dismutase (SOD), Complement C_3 activity, albumin content,
CER content is determined using the assay method for the kit that biotechnology research institute production is built up by Nanjing.
Table 2
| LSZ(U/ml) | CAT(U/ml) | SOD(U/ml) | Complement C_3 (U/ml) | |
| Test group 1 | 0.035±0.015a | 2.82±0.06a | 89.26±0.14a | 0.3541±0.008a |
| Test group 2 | 0.039±0.020a | 2.90±0.06b | 91.86±0.15b | 0.3624±0.007b |
| Test group 3 | 0.038±0.014a | 2.86±0.06a | 90.57±0.14b | 0.3555±0.005a |
| Test group 4 | 0.040±0.021b | 2.92±0.06b | 93.32±0.17b | 0.3589±0.006a |
| Test group 5 | 0.045±0.018b | 2.96±0.06b | 96.24±0.15b | 0.3682±0.008b |
| Test group 6 | 0.014±0.011c | 1.01±0.07c | 40.26±0.16c | 0.2013±0.005c |
Remarks：The different English alphabets that indicate in table after same row represent there is significant difference (P ＜ 0.05).
Lysozyme (LSZ) of the additive of Chinese herbal medicine used in this application to black bottom soft-shelled turtle, peroxidating as can be seen from the table
Hydrogen enzyme (CAT), superoxide dismutase (SOD), Complement C_3 increase significantly, and from low dose to being stepped up to big
Lysozyme (LSZ), catalase (CAT), superoxide dismutase (SOD), the growth trend of Complement C_3 are equal when dosage
Dropped afterwards first to increase, the amount for illustrating to add Chinese medicinal herb medicament is also required to reasonable control, in lysozyme compared with blank control
(LSZ), there is significant difference in catalase (CAT), superoxide dismutase (SOD), Complement C_3.
The detection of 1.3 pairs of blood physiological and biochemical indices
| Albumin content (mg/l) | CER content (U/l) | |
| Test group 1 | 21.21±1.25a | 42.12±1.01a |
| Test group 2 | 23.20±1.25a | 46.11±1.04a |
| Test group 3 | 27.31±1.25a | 49.30±1.03a |
| Test group 4 | 25.17±1.25a | 48.24±1.02a |
| Test group 5 | 29.11±1.25a | 50.05±1.00b |
| Test group 6 | 14.14±1.20b | 30.15±1.21c |
Remarks：The different English alphabets that indicate in table after same row represent there is significant difference (P ＜ 0.05).
The additive of Chinese herbal medicine used in this application is to albumin content, CER content as can be seen from the table
Compared from test group 1- test groups 5, albumin content, CER content first increase from low dosage to high dose to drop afterwards, is trying
The dosage of group 5 is tested to albumin content, the different work of most significant difference compared with blank control of CER content.
According to test group 1-6 breeding way, after feeding 3 months, test group 1-5 survival rate reaches 89 ± 6%, experiment
6 survival rates of group are 78%, and the death rate reduces 4-17%.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that come for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (5)
- A kind of 1. Chinese medicine composition for improving black bottom soft-shelled turtle immunity, it is characterised in that：Include following raw material in parts by weight It is prepared：40 parts -80 parts of frankincense, 30 parts -50 parts of caulis piperis futokadsurae, 30 parts -50 parts of kelp, 30 parts -40 parts of the Radix Astragali, the fleece-flower root 40 Parts -50 parts, 20 parts -30 parts of Chinese cassia tree, 20 parts -35 parts of aloe, 30 parts -40 parts of hawthorn, 15 parts -30 parts of Semen Cuscutae, 10 parts of caraway seed - 15 parts, 10 parts -20 parts of dark plum, 10 parts -20 parts of jujube, windproof 5 parts -15 parts, 10 parts -25 parts of radix glycyrrhizae.
- 2. the Chinese medicine composition according to claim 1 for improving black bottom soft-shelled turtle immunity, it is characterised in that：According to parts by weight Meter includes following raw material and is prepared：50 parts -70 parts of frankincense, 35 parts -45 parts of caulis piperis futokadsurae, 35 parts -40 parts of kelp, 33 parts of the Radix Astragali - 38 parts, 42 parts -48 parts of the fleece-flower root, 23 parts -27 parts of Chinese cassia tree, 25 parts -30 parts of aloe, 32 parts -37 parts of hawthorn, 20 part -27 of Semen Cuscutae Part, 11 parts -14 parts of caraway seed, 12 parts -18 parts of dark plum, 12 parts -18 parts of jujube, windproof 8 parts -12 parts, 15 parts -20 parts of radix glycyrrhizae.
- 3. the Chinese medicine composition according to claim 1 for improving black bottom soft-shelled turtle immunity, it is characterised in that：According to parts by weight Meter includes following raw material and is prepared：60 parts of frankincense, 40 parts of caulis piperis futokadsurae, 37 parts of kelp, 35 parts of the Radix Astragali, 45 parts of the fleece-flower root, Chinese cassia tree 25 parts, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, 12 parts of caraway seed, 14 parts of dark plum, 15 parts of jujube, windproof 10 parts, radix glycyrrhizae 18 parts.
- 4. the Chinese medicine composition according to claim 1 for improving black bottom soft-shelled turtle immunity, it is characterised in that：It is by claim Chinese medicine composition obtained by 1 adds the auxiliary material commonly used in pharmacy and is made, the formulation of the medicament is powder, pill or Granula.
- 5. the Chinese medicine composition according to claim 1 for improving black bottom soft-shelled turtle immunity, it is characterised in that：It is black applied to preparing In the trionyx sinensis feed additive of bottom.
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| CN104522375A (en) * | 2014-12-15 | 2015-04-22 | 惠州市财兴实业有限公司 | Young turtle compound feed for improving immunity and preparation method of young turtle compound feed |
| CN105010861A (en) * | 2015-07-20 | 2015-11-04 | 句容市郭庄镇南河农庄 | High immunity trionyx sinensis Chinese herbal medicine feed additive and preparation method and application thereof |
| CN107183365A (en) * | 2017-06-20 | 2017-09-22 | 徐州大名水产科技有限公司 | A kind of feed for improving soft-shelled turtle immunity and preparation method thereof |
| CN107581153A (en) * | 2017-10-31 | 2018-01-16 | 广西平南县平原农牧有限公司 | A kind of method of black bottom soft-shelled turtle ecologic breeding |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20090115609A (en) * | 2008-05-02 | 2009-11-05 | (주)아모레퍼시픽 | Medicinal plants extract using processing of herbal medicine and composition of skin external application comprising the same |
| CN104522375A (en) * | 2014-12-15 | 2015-04-22 | 惠州市财兴实业有限公司 | Young turtle compound feed for improving immunity and preparation method of young turtle compound feed |
| CN105010861A (en) * | 2015-07-20 | 2015-11-04 | 句容市郭庄镇南河农庄 | High immunity trionyx sinensis Chinese herbal medicine feed additive and preparation method and application thereof |
| CN107183365A (en) * | 2017-06-20 | 2017-09-22 | 徐州大名水产科技有限公司 | A kind of feed for improving soft-shelled turtle immunity and preparation method thereof |
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