CN107647933A - One kind is suitable to micro-administration method inside octopus section animal - Google Patents
One kind is suitable to micro-administration method inside octopus section animal Download PDFInfo
- Publication number
- CN107647933A CN107647933A CN201711116297.8A CN201711116297A CN107647933A CN 107647933 A CN107647933 A CN 107647933A CN 201711116297 A CN201711116297 A CN 201711116297A CN 107647933 A CN107647933 A CN 107647933A
- Authority
- CN
- China
- Prior art keywords
- octopus
- micro
- administration method
- flexible plastic
- section animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Abstract
The invention discloses one kind to be suitable to micro-administration method inside octopus section animal, first by octopus section animal deep anaesthesia, then in soft skin, subdermal muscle and the liver coating otch of the center back shaft position close to head；Nook closing member and flexible plastic sleeve are inserted by the main blood vessel in head using medical remaining needle together, nook closing member is pulled out, flexible plastic sleeve is stayed in main blood vessel；To organize glue to fix flexible plastic sleeve, by myometrial suture and closing, finally octopus is put into the oxygen-enriched circulating seawer of cleaning and recovered.Have the beneficial effect that：This is medication inside the first suitable octopus class animal established so far, and the skin in octopus, subdermal muscle and liver coating otch can make octopus comparatively fast heal, reduce the death rate of octopus with stress；Only need once it is embedding can continuous several times micro-administration and efficacy of medicine observing are carried out to octopus class, both reduced the cost of frequent operation, and reduced the damage to caused by octopus class again.
Description
Technical field
The present invention relates to micro-administration field in marine animal body, is suitable to more particularly, to one kind micro- inside octopus section animal
Measure medication.
Technical background
Octopus class（Octopodidae）, Mollusca（Mollusca）, Cephalopoda（Cephalopoda）, eight wrist catalogues
（Octopodiformes）, Pctopoda（Octopoda）A section, be a maximum section in Cephalopoda.It is generally called octopus.It is distributed in generation
Each marine site in boundary, there are about 140 kinds.Most of is shallow sea property species, also there is a small number of deep-sea property species.Octopus class is to be increased recent years
The species of exploitation is cultivated, with the development that its artificial breeding and aquaculture are developed, to the side such as its behaviouristics, physiology, pathology
The research in face also begins to be carried out extensively, the developments of these work be largely dependent upon internal injection neurotransmitter,
The chemical reagent such as hormone, medicine can be just smoothed out.Lack yet with siphonopods flourishing subcutaneous as mammal
Capillary system, thus bring difficulty to artificial administration.
The content of the invention
It is an object of the invention to provide one kind to be suitable to micro-administration method inside octopus section animal, and method can reduce octopus
The death rate with stress, while only need single treatment can continuous several times micro-administration and efficacy of medicine observing, cost are carried out to octopus class
Relatively low, the damage to octopus section animal is smaller.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of body suitable for octopus section animal
Interior micro-administration method, including：
Step 1：Octopus is put into the immersion 90-100 seconds in the seawater of ethanol containing 1.8-2.1% and 15.8-17.5 g/L magnesium chlorides, made
Octopus deep anaesthesia；To anaesthetize octopus containing the seawater of ethanol and magnesium chloride, octopus can be made to enter accurate narcosis, contribute to octopus machine
The preservation of vitality of subject and rapidly clear-headed；
Step 2：In the center back shaft position of the close head of octopus, cut in succession in soft skin, subdermal muscle and liver coating
Mouthful, kerf width 1.8-2.2cm；Skin, subdermal muscle and liver coating can comparatively fast heal, and reduce the death rate of octopus with answering
Swash；
Step 3：The main blood vessel in head is found, nook closing member and flexible plastic sleeve are inserted by the main blood vessel in head using medical remaining needle together,
Nook closing member is pulled out, only stays in very thin flexible plastic sleeve in main blood vessel；Flexible plastic sleeve is stayed in main blood vessel, passes through flexible plastic
Female Luer on sleeve pipe is administered, and because female Luer can be used for multiple times, thus this method can also realize the multiple of medicine
Administration, but is only once performed the operation, and makes it possible the effect of Continuous Observation medicine；
Step 4：Flexible plastic sleeve tissue glue's hydropexis, is administered, then liver by the female Luer on flexible plastic sleeve
Dirty coating, subdermal muscle and skin are sutured and closed with tissue glue, then octopus is put into the richness of cleaning at a temperature of 25-28 DEG C
Recovered in oxygen cycle seawater, octopus can regain consciousness and come into play after a few minutes；After the completion of embedding, octopus is positioned over oxygen-enriched follow
Being surrounded by sea in water can make it regain consciousness rapidly, micro-administration method to octopus class damage it is smaller, it is only necessary to it is once embedding can be continuous
Micro-administration and efficacy of medicine observing repeatedly are carried out to octopus class, the cost of frequent operation had both been reduced, and had reduced again to caused by octopus class
Damage, it is a kind of method for being suitable to micro-administration in octopus section animal body efficiently, safe.
Preferably, tissue glue is alpha-cyanacrylate adhesive or fibrin adhesive；Tissue glue is connecing
Polymerisation can occur rapidly after touching tissue fluid, wound, antibacterial bacteriostatic, waterproof and breathable, mitigation pain etc. can be closed rapidly and made
With the recovery of octopus section animal can be accelerated.
Compared with prior art, the advantage of the invention is that：1）This is the first micro-administration method of octopus class animal established,
Make it possible the invertebrate vivo medicine-feeding without subcutaneous capillary；2）Flexible plastic sleeve is stayed in main blood vessel, by soft
Female Luer on plastic bushing is administered, and because female Luer can be used for multiple times, thus this method can also realize medicine
Multiple dosing, micro-administration method are damaged smaller to octopus class, it is only necessary to once it is embedding can continuous several times octopus class micro give
Medicine and efficacy of medicine observing, had both reduced the cost of frequent operation, reduced the damage to caused by octopus class again.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Soaked 90 seconds 1. using octopus being put into the seawater containing 2% ethanol and 17.5 g/L magnesium chlorides first, make cuttlefish deep anaesthesia；
2. using scalpel in the successive otch of center back shaft position of the close head of octopus soft skin, subdermal muscle and liver
Coating, otch 1.8cm；
3. the main blood vessel in head is found, using medical remaining needle（21G syringe needles are smaller）By nook closing member and flexible plastic sleeve together
The main blood vessel in head is inserted, nook closing member is pulled out, only stays in very thin flexible plastic sleeve in main blood vessel；
4. flexible plastic sleeve tissue glue's hydropexis, then liver coating, subdermal muscle and skin are sutured and closed with tissue glue；
5. whole process continues 4-5 minutes, octopus is put into the oxygen-enriched circulating seawer of cleaning after operation and recovered.After a few minutes
Octopus can regain consciousness and come into play；
6. the female Luer that medicine is connected to by micro syringe on indwelling needle catheter is administered, because female Luer can be more
Secondary use, thus this method can also realize the multiple dosing of medicine, but only once performed the operation, make the effect of Continuous Observation medicine
It is possibly realized.
Embodiment 2：
One kind is suitable to micro-administration method inside octopus section animal, including：
1）Octopus is put into the seawater containing 2.1% ethanol and 17.5g/L magnesium chlorides and soaked 100 seconds, makes octopus deep anaesthesia；2）In octopus
Close head center back shaft position, be in soft skin, subdermal muscle and liver coating otch, kerf width in succession
2.2cm；3）The main blood vessel in head is found, nook closing member and flexible plastic sleeve are inserted by the main blood vessel in head using medical remaining needle together, pulled out
Except nook closing member, only very thin flexible plastic sleeve is stayed in main blood vessel；4）Flexible plastic sleeve tissue glue's hydropexis, passes through flexible plastic
Female Luer on sleeve pipe is administered, and then liver coating, subdermal muscle and skin are sutured and closed with tissue glue, then
Recovered in the oxygen-enriched circulating seawer that octopus is put into cleaning at a temperature of 28 DEG C, octopus can regain consciousness and start to live after a few minutes
It is dynamic；After the completion of embedding, octopus, which is positioned in oxygen-enriched circulating seawer, it is regained consciousness rapidly, and micro-administration method is to octopus class
Damage is smaller, it is only necessary to once it is embedding can continuous several times micro-administration and efficacy of medicine observing are carried out to octopus class, both reduced frequent behaviour
The cost of work, the damage to caused by octopus class is reduced again, be a kind of efficient, safety suitable for micro-administration in octopus section animal body
Method.
Embodiment 3：
One kind is suitable to micro-administration method inside octopus section animal, including：
Step 1：Octopus is put into containing 2.1% ethanol, 16.5g/L magnesium chlorides, the adenosine triphosphates of 0.35-0.39 ‰, 0.028-
0.033‰（-）-（1S, 4R）Soaked 95 seconds in the seawater of-camphanic acid, make octopus deep anaesthesia；With the seawater containing ethanol and magnesium chloride
Octopus is anaesthetized, octopus can be made to enter accurate narcosis, contributes to the preservation of octopus body energy and rapidly regains consciousness；Adenosine three
Phosphoric acid-magnesium chloride can have protective effect, simultaneously by blood-brain barrier to octopus section Animal Anoxia ischemic brain cell（-）-（1S, 4R）-
Camphanic acid can produce synergy with adenosine triphosphate, protection of the enhancing ATP magnesium chloride to brain cell, reduce brain
Cellular damage, accelerate octopus section animal from revival to active resume speed；
Step 2：In the center back shaft position of the close head of octopus, cut in succession in soft skin, subdermal muscle and liver coating
Mouthful, kerf width 2.0cm；Skin, subdermal muscle and liver coating can comparatively fast heal, reduce the death rate of octopus with stress；
Step 3：The main blood vessel in head is found, nook closing member and flexible plastic sleeve are inserted by the main blood vessel in head using medical remaining needle together,
Nook closing member is pulled out, only stays in very thin flexible plastic sleeve in main blood vessel；Flexible plastic sleeve is stayed in main blood vessel, passes through flexible plastic
Female Luer on sleeve pipe is administered, and because female Luer can be used for multiple times, thus this method can also realize the multiple of medicine
Administration, but is only once performed the operation, and makes it possible the effect of Continuous Observation medicine；
Step 4：Flexible plastic sleeve tissue glue's hydropexis, is administered, then liver by the female Luer on flexible plastic sleeve
Dirty coating, subdermal muscle and skin are sutured and closed with tissue glue, and octopus then is put into the oxygen-enriched of cleaning at a temperature of 26 DEG C
Recovered in circulating seawer, octopus can regain consciousness and come into play after a few minutes；After the completion of embedding, octopus is positioned over oxygen-enriched circulation
Rapidly it can be made to regain consciousness in seawater, micro-administration method to octopus class damage it is smaller, it is only necessary to it is once embedding can be continuously more
It is secondary that micro-administration and efficacy of medicine observing are carried out to octopus class, the cost of frequent operation had both been reduced, has reduced the damage to caused by octopus class again
Wound, it is a kind of method for being suitable to micro-administration in octopus section animal body efficiently, safe.
By naked-eye observation, the octopus section animal for implementing internal micro-administration in embodiment 1-3 is subjected to comprehensive evaluating, point
Do not evaluated and tested in terms of recovery time, active time, health status, as a result as shown in table 1.
The recovery situation evaluation and test of the embodiment 1-3 Zhong Xiao sections animal of table 1.
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Recovery time/minute | 8 | 7 | 8 |
| Active time/minute | 12 | 11 | 6 |
| Health status | Well | Well | It is excellent |
As shown in Table 1, octopus section animal after micro-administration in body is in a good state of health, and its revival and active
Time is shorter, and it is a kind of safe, efficient suitable for micro-administration method inside octopus section animal to illustrate this method.Especially,
Compared to embodiment 1-2, the active time in embodiment 3 substantially reduces, and its health status is also preferable.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Claims (6)
1. one kind is suitable to micro-administration method inside octopus section animal, it is characterised in that：Described micro-administration method includes：
Step 1：Octopus is put into the seawater containing ethanol and magnesium chloride and soaked, makes octopus deep anaesthesia；
Step 2：In the center back shaft position of the close head of octopus, cut in succession in soft skin, subdermal muscle and liver coating
Mouthful；
Step 3：The main blood vessel in head is found, nook closing member and flexible plastic sleeve are inserted by the main blood vessel in head using medical remaining needle together,
Nook closing member is pulled out, only stays in very thin flexible plastic sleeve in main blood vessel；
Step 4：Flexible plastic sleeve tissue glue's hydropexis, can repeatedly be given by the female Luer on flexible plastic sleeve
Medicine, liver coating, subdermal muscle and skin are sutured and closed with tissue glue, then octopus is put into the oxygen-enriched circulating seawer of cleaning
Middle recovery.
It is 2. according to claim 1 a kind of suitable for micro-administration method inside octopus section animal, it is characterised in that：It is described
The content of ethanol is 1.8-2.1% in seawater in step 1 for anaesthetizing octopus section animal, and the content of magnesium chloride is 15.8-17.5
g/L。
It is 3. according to claim 1 a kind of suitable for micro-administration method inside octopus section animal, it is characterised in that：The step
The time that octopus section animal is anaesthetized in rapid one is the 90-100 seconds.
It is 4. according to claim 1 a kind of suitable for micro-administration method inside octopus section animal, it is characterised in that：The step
Kerf width in rapid two is 1.8-2.2cm.
It is 5. according to claim 1 a kind of suitable for micro-administration method inside octopus section animal, it is characterised in that：The step
Tissue glue in rapid four is alpha-cyanacrylate adhesive or fibrin adhesive.
It is 6. according to claim 1 a kind of suitable for micro-administration method inside octopus section animal, it is characterised in that：The step
The temperature of Zhou Sizhongxiao sections animal revival is 25-28 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711116297.8A CN107647933A (en) | 2017-11-13 | 2017-11-13 | One kind is suitable to micro-administration method inside octopus section animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711116297.8A CN107647933A (en) | 2017-11-13 | 2017-11-13 | One kind is suitable to micro-administration method inside octopus section animal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107647933A true CN107647933A (en) | 2018-02-02 |
Family
ID=61121081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711116297.8A Pending CN107647933A (en) | 2017-11-13 | 2017-11-13 | One kind is suitable to micro-administration method inside octopus section animal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107647933A (en) |
-
2017
- 2017-11-13 CN CN201711116297.8A patent/CN107647933A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Anderson | Practical light embalming technique for use in the surgical fresh tissue dissection laboratory | |
| US20170215411A1 (en) | Administration and monitoring of nitric oxide in ex vivo fluids | |
| SNYDER et al. | Extremity replantation | |
| CN205758657U (en) | A kind of blood vessel dredge | |
| Liu et al. | Neurologic recovery after deep hypothermic circulatory arrest in rats: A description of a long‐term survival model without blood priming | |
| CN104096274B (en) | Free skin flap external amphi position blood supply device | |
| CN107647933A (en) | One kind is suitable to micro-administration method inside octopus section animal | |
| CN203075346U (en) | Infant intra-arterial infusion syringe needle | |
| CN108359604A (en) | A kind of conveying of stem cell seed and microenvironment regulating system | |
| Evans | Observations on the poisoned spines of the weever fish (Trachinus draco) | |
| Abayomi et al. | Embalmment: a veritable source of human body preservation | |
| Lasa et al. | Saline solutions in history | |
| Andrews et al. | A technique for central drug administration in Octopus vulgaris | |
| Langnas | The history of intestinal failure and transplantation | |
| Hartley et al. | BS14 First demonstration of a novel experimental model of atherosclerosis. The ex vivo on-ecmo amputated ‘living’human limb model | |
| CN106510887A (en) | Method for constructing novel aortic valve disease small pig animal model | |
| An et al. | Demonstration of intramuscular artery and nerve distribution in the same whole mount gracilis muscle by different colors | |
| CN101301227A (en) | Method for installing tube in rat spinal dural external cavity and subarachnoid cavity | |
| CN104398313A (en) | Method for making aortic endothelial cell injury model by utilizing mice and detection method | |
| Cox | The functional significance and evolution of the coronary circulation in sharks | |
| NL1041300B1 (en) | Double cannula. | |
| JP5840193B2 (en) | Infusion solution for cadaver preservation | |
| Richardson | Researches on the Treatment of Suspended Animation | |
| Merritt et al. | The effects of radiation therapy in Clostridium infection in sheep | |
| CA2885165A1 (en) | Method of drug testing incorporating the use of vascularized tissues perfused by exvivo attachment to a living organism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180202 |
|
| RJ01 | Rejection of invention patent application after publication |