CN107646700A - A kind of recessed leaf melon fragrance wood culture medium - Google Patents
A kind of recessed leaf melon fragrance wood culture medium Download PDFInfo
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- CN107646700A CN107646700A CN201711126906.8A CN201711126906A CN107646700A CN 107646700 A CN107646700 A CN 107646700A CN 201711126906 A CN201711126906 A CN 201711126906A CN 107646700 A CN107646700 A CN 107646700A
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- CN
- China
- Prior art keywords
- culture medium
- melon fragrance
- recessed
- leaf melon
- recessed leaf
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of recessed leaf melon fragrance wood culture medium, including following component：1/4MS culture mediums, 0.5 1mg/L gibberellin, 0.3 0.8mg/L6-benzylaminopurine, 1.2 1.5mg/L brassins, 0.3 0.5 triacontanols, 0.08 0.2mg/L diethyl aminoethyl hexanoates, 0.05 0.1mg/L triadimefons, 6 10g/L agaroses.The present invention with the addition of gibberellin, 6-benzylaminopurine and triacontanol on the basis of MS culture mediums, growth to recessed leaf melon fragrance wood cauline leaf bud serves splendid facilitation, the wood growth of recessed leaf melon fragrance is adjusted, prevent local nutrient imbalance, influence survives, triadimefon is added simultaneously to carry out plant sterilization, prevent pest and disease damage, effectively improves the survival rate in recessed leaf melon fragrance wood cultivating process, there is extremely important meaning to drug field.
Description
Technical field
The present invention relates to plant tissue culture technique, and in particular to a kind of recessed leaf melon fragrance wood culture medium.
Background technology
Wooden (the scientific name of recessed leaf melon fragrance:Fissistigma retusum) it is Annonaceae Fissistigma species, for the spy of China
There is plant.Concrete form is as follows：Sprig is by brown fine hair.Leaf keratin or nearly keratin, wide avette, obovate or ovate is oval
Shape, long 9-26 centimetres, wide 4.5-13 centimetres, top circle or nick, basal circular to section shape, sometimes in shallow heart, blade face is only
Middle arteries and lateral vein tomentellate, blade back is recessed by brown fine hair, lateral vein per side 15-20 bars on blade face, in blade back projection, net vein
Substantially, with the nearly vertical web knot of lateral vein；The long 8-15 millimeters of petiole, tomentellate, there is groove above.Hua Duoduo forms glomerule, flower
Sequence is with leaf to life；The long 5-10 millimeters of total bennet；Several no bennets；Sepal ovum shape lanceolar, is about 1 centimetre, with petal during bud etc.
Long, top is tapering, outside tomentellate, and inner face is hairless；Foreign steamer petal ovate Long Circle, is about 1.5 centimetres, outside is by short flannel
Hair, inner face is hairless, and lubrication groove petal ovum shape lanceolar, shorter than foreign steamer petal, base portion is curved slightly within, and two sides is hairless；The wealthy triangle of connective；
Carpel grows 1.5 millimeters, and close by thin,tough silk matter pubescence, style Long Circle is interior curved, by pubescence, column cap top full edge, has ovule 4 per carpel
, 2 rows.Fruit spherical shape, about 3 centimetres of diameter, by golden yellow down；Carpopodium grows 1.5 centimetres, by golden yellow down.
Recessed leaf melon fragrance wood is grown on 340 meters to 2 of height above sea level, and 000 meter of area is how raw in the thick forest of mountain region, has higher
Medical value, but at present not yet by artificial introducing and planting, therefore, the culture medium for developing its suitable growth is that we are badly in need of solving
Certainly the problem of.
The content of the invention
It is an object of the invention to provide a kind of recessed leaf melon fragrance wood for promoting growth, reducing pest and disease damage while improving survival rate
Culture medium.
The technical solution for realizing the object of the invention is：
A kind of recessed leaf melon fragrance wood culture medium, including following component：1/4MS culture mediums, 0.5-1mg/L gibberellin, 0.3-
0.8mg/L6-benzylaminopurine, 1.2-1.5mg/L brassins, 0.3-0.5 triacontanols, 0.08-0.2mg/L diethyl aminoethyl hexanoates,
0.05-0.1mg/L triadimefons, 6-10g/L agaroses, the pH value of the culture medium is 6-7.
In further preferred embodiment, the recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums,
0.6mg/L gibberellin, 0.7mg/L6-benzylaminopurine, 1.3mg/L brassins, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates,
0.08mg/L triadimefons, 8g/L agaroses, the pH value of the culture medium is 6-7.
Compared with prior art, its remarkable advantage is the present invention：
The present invention with the addition of gibberellin, 6-benzylaminopurine and triacontanol on the basis of MS culture mediums, to recessed leaf
The growth of melon fragrance wood cauline leaf bud serves splendid facilitation, and by adding diethyl aminoethyl hexanoate, brassin grows to recessed leaf melon fragrance wood
It is adjusted, prevents local nutrient imbalance, influence to survive, while adds triadimefon and carry out plant sterilization, prevent pest and disease damage,
To sum up, culture medium of the present invention can effectively improve the survival rate in recessed leaf melon fragrance wood cultivating process, have pole to drug field
Its great meaning.
Embodiment
The present invention is described in further detail with reference to specific embodiment.
Embodiment 1
A kind of recessed leaf melon fragrance wood culture medium, including following component：1/4MS culture mediums, 0.5mg/L gibberellin, 0.3mg/
L6-benzylaminopurine, 1.2mg/L brassins, 0.5 triacontanol, 0.08mg/L diethyl aminoethyl hexanoates, 0.1mg/L triadimefons, 6g/L
Agarose, the pH value of the culture medium is 6-7.
Embodiment 2
A kind of recessed leaf melon fragrance wood culture medium, including following component：1/4MS culture mediums, 1mg/L gibberellin, 0.8mg/L6-
Benzylaminopurine, 1.5mg/L brassins, 0.3 triacontanol, 0.2mg/L diethyl aminoethyl hexanoates, 0.05mg/L triadimefons, 10g/L agar
Sugar, the pH value of the culture medium is 6-7.
Embodiment 3
A kind of recessed leaf melon fragrance wood culture medium, including following component：1/4MS culture mediums, 0.6mg/L gibberellin, 0.8mg/
L6-benzylaminopurine, 1.3mg/L brassins, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 0.1mg/L triadimefons, 9g/L fine jades
Lipolysaccharide, the pH value of the culture medium is 6-7.
Embodiment 4
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 0.7mg/
L6-benzylaminopurine, 1.3mg/L brassins, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 0.08mg/L triadimefons, 8g/L
Agarose, the pH value of the culture medium is 6-7.
Following six groups of control groups are prepared to test as a comparison：
Control group 1
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.7mg/L6-benzylaminopurine,
1.3mg/L brassins, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 0.08mg/L triadimefons, 8g/L agaroses, the culture medium
PH value be 6-7.
Control group 2
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 1.3mg/L rues
Tongue element, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 0.08mg/L triadimefons, 8g/L agaroses, the pH value of the culture medium is 6-
7。
Control group 3
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 0.7mg/
L6-benzylaminopurine, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 0.08mg/L triadimefons, 8g/L agaroses, the culture
The pH value of base is 6-7.
Control group 4
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 0.7mg/
L6-benzylaminopurine, 1.3mg/L brassins, 0.1mg/L diethyl aminoethyl hexanoates, 0.08mg/L triadimefons, 8g/L agaroses, the training
The pH value for supporting base is 6-7.
Control group 5
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 0.7mg/
L6-benzylaminopurine, 1.3mg/L brassins, 0.4 triacontanol, 0.08mg/L triadimefons, 8g/L agaroses, the culture
The pH value of base is 6-7.
Control group 6
A kind of recessed leaf melon fragrance wood culture medium, including following components：1/4MS culture mediums, 0.6mg/L gibberellin, 0.7mg/
L6-benzylaminopurine, 1.3mg/L brassins, 0.4 triacontanol, 0.1mg/L diethyl aminoethyl hexanoates, 8g/L agaroses, the culture
The pH value of base is 6-7.
Breeding method：
The recessed leaf melon fragrance wood tender shoots of clip 2-3cm length goes to above-described embodiment and the culture medium of control group is in intensity of illumination
500lux, temperature are cultivated under conditions of being 25 ± 1 DEG C, and the data obtained see the table below：
Claims (2)
1. a kind of recessed leaf melon fragrance wood culture medium, it is characterised in that including following component：
1/4MS culture mediums,
0.5-1mg/L gibberellin,
0.3-0.8mg/L6-benzylaminopurine,
1.2-1.5mg/L brassins,
0.3-0.5 triacontanols,
0.08-0.2mg/L diethyl aminoethyl hexanoates,
0.05-0.1mg/L triadimefons,
6-10g/L agaroses,
The pH value of the culture medium is 6-7.
2. a kind of recessed leaf melon fragrance wood culture medium according to claim 1, it is characterised in that including following components：
1/4MS culture mediums,
0.6mg/L gibberellin,
0.7mg/L6-benzylaminopurine,
1.3mg/L brassins,
0.4 triacontanol,
0.1mg/L diethyl aminoethyl hexanoates,
0.08mg/L triadimefons,
8g/L agaroses,
The pH value of the culture medium is 6-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711126906.8A CN107646700A (en) | 2017-11-15 | 2017-11-15 | A kind of recessed leaf melon fragrance wood culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711126906.8A CN107646700A (en) | 2017-11-15 | 2017-11-15 | A kind of recessed leaf melon fragrance wood culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107646700A true CN107646700A (en) | 2018-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711126906.8A Pending CN107646700A (en) | 2017-11-15 | 2017-11-15 | A kind of recessed leaf melon fragrance wood culture medium |
Country Status (1)
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| CN (1) | CN107646700A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042338A (en) * | 2018-10-17 | 2018-12-21 | 徐颂涛 | One kind preventing sky seedling watermelon seedling cultural method |
| CN112021177A (en) * | 2020-08-26 | 2020-12-04 | 济宁市农业科学研究院 | Active synergist for improving rapid breeding of purple sweet potato tissue culture seedlings and application thereof |
-
2017
- 2017-11-15 CN CN201711126906.8A patent/CN107646700A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042338A (en) * | 2018-10-17 | 2018-12-21 | 徐颂涛 | One kind preventing sky seedling watermelon seedling cultural method |
| CN112021177A (en) * | 2020-08-26 | 2020-12-04 | 济宁市农业科学研究院 | Active synergist for improving rapid breeding of purple sweet potato tissue culture seedlings and application thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20180202 |