CN107641603A - A kind of method for producing light, low ash content yeast - Google Patents
A kind of method for producing light, low ash content yeast Download PDFInfo
- Publication number
- CN107641603A CN107641603A CN201710951916.9A CN201710951916A CN107641603A CN 107641603 A CN107641603 A CN 107641603A CN 201710951916 A CN201710951916 A CN 201710951916A CN 107641603 A CN107641603 A CN 107641603A
- Authority
- CN
- China
- Prior art keywords
- yeast
- molasses
- sugar
- ash content
- low ash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of method for producing light, low ash content yeast, methods described includes the steps such as the classified estimation to yeast fermentation raw material cane molasses, dilution acidifying, precipitation, centrifugation, sterilizing successively, and flocculation sediment, resin decolorization, hydrolysis sugar or the syrup mixed fermentation that reconciles are further comprised between precipitation and separating step.This method causes on the basis of keeping original zymotechnique constant, at utmost reduce yeast fermentation raw material impurity content, improve yeast fermenting power and yield, light, low-ash high nutritive value yeast product is obtained, meets food higher to yeast product color, ash requirements on the market, health products and the demand for protecting cosmetics.
Description
Technical field
The invention belongs to technical field of microbial fermentation, more particularly to a kind of method for producing light, low ash content yeast.
Background technology
Accessory substance of the cane molasses as sugar industry, is a kind of comparatively ideal yeast fermentation raw material, and its composition is very multiple
It is miscellaneous.Both containing the sugar favourable to yeast growth, inorganic matter, growth factor etc. in raw sugar honey, also containing a large amount of caramel, amino sugar
The harmful substances such as colloidal substance, about 10%~15% sulfated ass, VFA, microorganism Deng melanin.These
The presence of harmful substance not only has a strong impact on yeast metabolism so that yeast yield and quality reduce；And it is difficult to by microorganism
Degraded, and as fermented waste fluid discharges, this kind of COD and BOD height, the high-concentration organic substance wastewater of color depth cause very big to environment
Pollution.
Traditional molasses clarification removal of impurities typically by dilution, acidifying, heating ventilation, stand clarification or mechanically decoupled clarification,
The processing methods such as sterilizing.Organic non-sugar such as nitrogenous thing, soluble colloid in blackstrap, insoluble colloid and salts substances are normal
It is present in suspension, emulsion and colloidal solution state in molasses, colloid protein is gone in high-temperature heating process by condensing
Remove, diluting and being acidified can also make negatively charged colloid be flocculated under isoelectric point state a part, but due to flocculation rate slowly,
Wadding is heavy ineffective, and flocculate is also difficult to centrifuge, and causes the impurity particle that largely suspends and colloidal substance can not be effective
Remove.The colloidal substance of residual carries the electric charge opposite with yeast cells, and they can be adsorbed in yeast cells during the fermentation
Surface, have a strong impact on the metabolism of yeast, reduce the yield and quality of yeast, and can make dark-grey on yeast band
Color, influence the color and luster and quality of yeast.
A variety of ameliorative processing methods are used or had attempted both at home and abroad at present, such as deposition method, absorption method, membrane processing method
Deng.But the above method considers certain defect all be present from technical, economy, practicality and the feature of environmental protection, industry popularization and application by
To limitation.Though deposition method can be such that pigment in molasses substantially reduces, sugared part rate of recovery is very low, can only achieve 80%, and produce
In to add the ethanol of volume more than about 3 times, increase production cost；When absorption method handles molasses, molasses percent of decolourization is very high, reachable
More than 90%, but activated carbon dosage is more, and processing time is also longer, and secondary pollution is easily caused during regenerating active carbon, handling
With height.Application number CN201510705595.5 patent discloses a kind of atlapulgite and wood substance grain activated carbon combination to molasses
The method for carrying out removal of impurities decolouring, molasses are decolourized using atlapulgite and wood substance grain activated carbon in yeast actual production
There is certain help, but consider from cost performance, cost is too high；Membrane technology prospect is preferable, and sugared part rate of recovery is high, secondary dirt
Contaminate less, the liquid glucose yeast growth preferably after processing, yield and quality can be improved, the chroma in waste water and CODcr separated
Also there is obvious reduction, and biochemical property is improved, it is more disposable, but to put into huge cost of equipment, fortune using presence at present
Turn over journey and need to overcome membrane material short life, cost energy consumption is higher, operational administrative is complicated etc. is difficult to the economy and skill that solve at once
Art problem.
The country has factory to use chemical flocculation to reach the purpose of rapid impurity removal purification molasses, and common flocculant is poly-
Acrylamide.But polyacrylamide has a certain amount of residual monomer as organic polymer coargulator, its mechanism, no
It can avoid that toxicity can be brought, therefore application of this processing method in food processing industry is also subject to certain restrictions.And flocculate
Method needs the larger container of supporting volume, and floor space is big, and flocculant consumption is big, and heavy rear caused sludge of wadding a quilt with cotton can cause secondary dirt
Dye.
With flourishing for yeast industry, molasses seasonality production problem, the otherness of different sources quality, supply and demand are competing
Strive the problems such as fierce, Spirulina pollutes and constrain the optimization of yeast production cost and the lifting of product quality.Find one
Safe efficient, the simple and feasible raw material molasses processing method of kind, while do not influence subsequent processes and change with nutritional ingredient, it appears
It is particularly important.
The content of the invention
To solve the problems, such as that prior art is present, the present invention provides a kind of method for producing light, low ash content yeast, passes through
The steps such as classified estimation, dilution acidifying, precipitation, centrifugation, sterilizing are carried out to yeast fermentation raw material (cane molasses), are being precipitated
Further comprise flocculation sediment, resin decolorization, hydrolysis sugar or the syrup mixed fermentation that reconciles between separating step, keeping original
On the basis of zymotechnique is constant, yeast fermentation raw material impurity content is at utmost reduced, yeast fermenting power and yield is improved, obtains
To light, low-ash high nutritive value yeast product, meet food higher to yeast product color, ash requirements on the market
Product, health products and the demand for protecting cosmetics.
To achieve the above object, the present invention uses following technical scheme：
A kind of method for producing light, low ash content yeast, it is characterised in that the described method comprises the following steps：(1) molasses
Dilution；(2) acid adjustment；(3) heating ventilation；(4) clarification is stood；(5) centrifuge, take supernatant；(6) flash distillation sterilizing；(7) send out
Ferment；(8) refine, obtain yeast.
Preferably, methods described also includes：In step (5), into obtained supernatant add amylum hydrolysate of the sugar and/or
Mediation syrup.
Preferably in methods described is additionally included in step (3) and carries out addition flocculating settling agent before step (4) afterwards.
Preferably, the flocculating settling agent is Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate is by weight 1-2:
The built-up flocculant that 1-3 compoundings form.
Preferably, the addition of the flocculating settling agent is concentration expressed in percentage by volume 0.01-0.2 ‰.
Preferably, methods described also includes, and before step (5) carries out step (6) afterwards, carries out at ion-exchange resin decolorization
Enter after reason in surge tank, amylum hydrolysate of the sugar and/or mediation syrup are then added into surge tank.
Preferably, the addition of the amylum hydrolysate of the sugar and/or mediation syrup is the 35-45% of total sugar amount.
Preferably, the amylum hydrolysate of the sugar is sugar content>90% glucidtemns.
Preferably, the mediation syrup includes 50-90% glucose and 10-50% sucrose.
Preferably, in step (1), molasses are diluted to 30~40 ° of Bx.
Preferably, in step (2), pH to 3.5~4.5 is adjusted.
In order to more efficiently handle molasses, present invention also offers a kind of molasses grade scale, the stage division such as institute of table 1
Show.
The molasses grade scale of table 1
| Molasses rank | Total reducing sugar % | Purity | Colour | Sulfated ass % |
| One-level | 50 | ≥60 | ≤18000 | ≤10 |
| Two level | 48~50 | 55~59 | 18000~22000 | 10~12 |
| Three-level | ＜ 48 | ≤55 | ≥22000 | ≥12 |
Note：Purity, the detection method of sulfated ass (referring to Q/BT 2684-2005 cane molasses)；
Colour：In ICUMSA methods 2 (wavelength=560nm) for evaluation criterion
Specifically, the invention provides handling process and zymotechnique corresponding to different stage molasses.
(1) the processing zymotechnique of one-level molasses, comprises the following steps：
(1) molasses dilute；(2) acid adjustment；(3) heating ventilation；(4) clarification is stood；(5) centrifuge, take supernatant；(6)
Flash distillation sterilizing；(7) ferment；(8) refine, obtain yeast.
(2) two level molasses (it is relatively low belonging to purity, impurity content is higher, and colour is higher, the higher molasses of sulfated ass)
Zymotechnique is handled, is comprised the following steps：
(1) molasses dilute；(2) acid adjustment；(3) heating ventilation；(4) clarification is stood；(5) centrifuge, take supernatant；(6)
Amylum hydrolysate of the sugar and/or mediation syrup are added in supernatant；(7) flash distillation sterilizing；(8) ferment；(9) refine, obtain yeast.
(3) three-level molasses (belong to the raw material of molasses poor quality, impurity is more, purity is low, colour is deep, sulfated ass is high
Molasses) processing zymotechnique, comprise the following steps：
(1) molasses dilute；(2) acid adjustment；(3) heating ventilation；(4) addition precipitating reagent is flocculated；(5) clarification is stood；(6)
Centrifuge, take supernatant；(7) supernatant passes through ion-exchange resin decolorization；(8) enter surge tank, add amylum hydrolysate of the sugar
And/or mediation syrup；(9) flash distillation sterilizing；(10) ferment；(11) refine, obtain yeast.
The concrete scheme of the present invention is to carry out processing fermentation to one-level molasses, specifically includes following steps：
(1) molasses are diluted to 30~40 ° of Bx, obtain molasses dilution, allocate advantageously reducing molasses to the concentration and glue
Degree and relative density, it is easy to separate impurity, improves post sterilization effect；
(2) sulfuric acid or phosphoric acid is added to adjust the dilute liquid glucose pH value of molasses to 4.0~4.5, on this condition, due to the positive electricity in acid
Lotus is interacted with negatively charged molasses colloid, and flocculation sediment is formed during colloidal substance reaches isoelectric point；Simultaneously
Acid adding can promote volatile acid to discharge；
(3) the dilute liquid glucose of the molasses of adjusted pH value is heated and divulges information and be warming up to 80~90 DEG C；Heating can promote dilute liquid glucose
In colloid protein denaturation solidification, accelerate flocculation sediment；Filtrated air is continually fed into toward settling tank in heating process, on the one hand
Drive away volatile acid, on the other hand ensure the fully oxidized precipitation of impurity, avoid, because oxidation does not thoroughly cause impurity to be brought into fermentation, gluing
Yeast cell surface is attached to, influences the metabolism of yeast normal growth so that finished product color burnt hair；
(4) insulation stands 4~8h of clarification；
(5) the dilute liquid glucose of molasses after clarification is stood using the separation of molasses centrifuge, takes supernatant, reach clarification purification effect
Fruit；Further comprise precipitation slag processing step before centrifugation：The upper pellucid syrup pump after clarifying to molasses will be stood to centrifuge
Machine machine is separated, and the precipitation slag in settling tank is handled through decanting machine, the dilute liquid glucose remained in slag is at utmost reclaimed, under can be used as
The allotment water of secondary molasses dilution, reduces water consume, improves sugar overall recovery；
(6) supernatant after vaporing away organic acid and other pernicious gases, changes through 118 DEG C~125 DEG C flash distillation processes through board-like
Hot device is cooled to 50 DEG C~60 DEG C and used for fermentation；Liquid glucose through handling well is temporarily deposited in storage tank, selects 50 DEG C~60 DEG C
It is the probability for avoiding increasing under normal temperature condition microorganism secondary pollution；
(7) by through the good 2000~3000L of liquid glucose of above-mentioned PROCESS FOR TREATMENT, basic nutrient solution (liquid containing ammonium sulfate, phosphoric acid hydrogen two
Ammonium, magnesium sulfate, phosphoric acid, potassium dihydrogen phosphate etc.) 200~300L, 5~10m of process water3Ratio fermented, fermentation temperature is
26~32 DEG C, pH is 3.8~5.5.Culture yeasts is separated after fermentation ends, extracted, dries acquisition yeast product.
Another concrete scheme of the present invention is to carry out processing fermentation to two level molasses, specifically includes following steps：
(1) molasses are diluted to 30~40 ° of Bx, obtain molasses dilution, allocate advantageously reducing molasses to the concentration and glue
Degree and relative density, it is easy to separate impurity, improves post sterilization effect；
(2) sulfuric acid or phosphoric acid is added to adjust the dilute liquid glucose pH value of molasses to 3.8~4.2, on this condition, due to the positive electricity in acid
Lotus is interacted with negatively charged molasses colloid, and flocculation sediment is formed during colloidal substance reaches isoelectric point；Simultaneously
Acid adding can promote volatile acid to discharge；
(3) the dilute liquid glucose of the molasses of adjusted pH value is heated and divulges information and be warming up to 80~90 DEG C；Heating can promote dilute liquid glucose
In colloid protein denaturation solidification, accelerate flocculation sediment；Filtrated air is continually fed into toward settling tank in heating process, on the one hand
Drive away volatile acid, on the other hand ensure the fully oxidized precipitation of impurity, avoid, because oxidation does not thoroughly cause impurity to be brought into fermentation, gluing
Yeast cell surface is attached to, influences the metabolism of yeast normal growth so that finished product color burnt hair；
(4) insulation stands 4~8h of clarification；
(5) the dilute liquid glucose of molasses after clarification is stood using the separation of molasses centrifuge, takes supernatant, reach clarification purification effect
Fruit；Further comprise precipitation slag processing step before centrifugation：The upper pellucid syrup pump after clarifying to molasses will be stood to centrifuge
Machine machine is separated, and the precipitation slag in settling tank is handled through decanting machine, the dilute liquid glucose remained in slag is at utmost reclaimed, under can be used as
The allotment water of secondary molasses dilution, reduces water consume, improves sugar overall recovery；
(6) Starch Hydrolysis liquid glucose and/or the syrup that reconciles are diluted to 30~40 ° of Bx.By Starch Hydrolysis liquid glucose and/or the sugar that reconciles
Slurry dilution is pumped into molasses dilution supernatant in proportion, obtains mixed sugar liquid；The amylum hydrolysate of the sugar is starch through double
The product of enzymatic hydrolysis, containing more than 90% sugar；The syrup that reconciles is the byproduct for producing FOS product, is mainly contained
50-90% glucose and 10-50% sucrose, both sugar are of light color, content of ashes is low, by adding amylum hydrolysate of the sugar
And/or reconcile syrup and can not only increase fermentable sugar in molasses, it can also reduce or eliminate molasses colour, ash grades ripple
The dynamic influence to production process and product quality, improve fermentation production rate；The addition of hydrolysis sugar and/or mediation syrup is total sugar amount
During 35-45% or so, yeast yield increase about 9.0%, but after addition continues increasing, because trace element and ore deposit in molasses
Material is diluted, and content is reduced, and the production and synthesis for causing yeast are affected, and yield declines.If hydrolysis sugar is increased again
Content needs the mineral matters such as appropriate increase sodium, magnesium, potassium, phosphorus, zinc, iron, biotin, thiamine and trace element just to can guarantee that yeast
Normal growth metabolism, just can guarantee that yield and quality.During process implementing, according to the colour of molasses, sulfated ass index
Situation substitutes the addition of sugar (hydrolysis sugar or the syrup that reconciles) to adjust.The colour and sulfated ass of molasses acidifying solution are sugared with substituting
Add and reduce, the sulfated ass of acidifying solution is reduced to less than 4.5%, while improve the fermentability sugared content of acidifying solution；
(7) mixed sugar liquid is through 118 DEG C~125 DEG C flash distillation processes, after vaporing away organic acid and other pernicious gases, through board-like
Heat exchanger is cooled to 50 DEG C~60 DEG C and used for fermentation；Liquid glucose through handling well is temporarily deposited in storage tank, selects 50 DEG C~60
DEG C it is the probability for avoiding increasing under normal temperature condition microorganism secondary pollution；
(8) by through the good 2000~3000L of mixed sugar liquid of above-mentioned PROCESS FOR TREATMENT, basic nutrient solution (liquid containing ammonium sulfate, phosphoric acid hydrogen
Two ammoniums, magnesium sulfate, phosphoric acid, potassium dihydrogen phosphate etc.) 200~300L, mineral matter and liquid microelement (containing sodium, zinc, iron, biotin,
Thiamine, inositol etc.) 50~100ml, 5~10m of process water3Ratio fermented, fermentation temperature is 26~32 DEG C, and pH is
3.8~5.5.Culture is separated to culture yeasts after terminating, extracted, drying light, the low ash content yeast product of acquisition.
The another concrete scheme of the present invention is to carry out processing fermentation to three-level molasses, specifically includes following steps：
(1) molasses are diluted to 30~40 ° of Bx, obtain molasses dilution, allocate advantageously reducing molasses to the concentration and glue
Degree and relative density, it is easy to separate impurity, improves post sterilization effect；
(2) sulfuric acid or phosphoric acid is added to adjust the dilute liquid glucose pH value of molasses to 3.5~4.2, on this condition, due to the positive electricity in acid
Lotus is interacted with negatively charged molasses colloid, and flocculation sediment is formed during colloidal substance reaches isoelectric point；Simultaneously
Acid adding can promote volatile acid to discharge；
(3) the dilute liquid glucose of the molasses of adjusted pH value is heated and divulges information and be warming up to 80~90 DEG C；Heating can promote dilute liquid glucose
In colloid protein denaturation solidification, accelerate flocculation sediment；Filtrated air is continually fed into toward settling tank in heating process, on the one hand
Drive away volatile acid, on the other hand ensure the fully oxidized precipitation of impurity, avoid, because oxidation does not thoroughly cause impurity to be brought into fermentation, gluing
Yeast cell surface is attached to, influences the metabolism of yeast normal growth so that finished product color burnt hair；
(4) addition 0.01-0.2 ‰ (w/v) built-up flocculant is added into the dilute liquid glucose of molasses after heating is divulged information,
Agitator persistently stirs 15min with 200r/min, stands 2~4h of clarification, can effectively accelerate flocculation rate in molasses slow
Colloidal impurity conglomeration sedimentation, improve molasses dilution centrifugal efficiency and effect, so as to reduce the content of ashes in dilute liquid glucose；
The built-up flocculant used in the present invention is Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate is by weight 1-2:1-3 is answered
With being 1 into, it is preferable that the weight ratio of Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate:3, wherein alum is inorganic wadding
Solidifying agent, Sodium Polyacrylate are organic flocculants, two kinds of flocculant synergies, can produce compression double electric layer, make outstanding in waste water
Floating particulate loss of stability, micelle thing phase inter coagulation increase particulate, form floccule body, after floccule body is grown up to certain volume i.e.
Depart from aqueous phase precipitation under gravity, so as to remove a large amount of suspensions in molasses.Provided in GB 2760-2014 in food
Aluminium content must not be higher than 100mg/kg, and the aluminium content contained in the present invention in built-up flocculant used in alum will far below regulation
Ask.Sodium Polyacrylate is the food additives that the approvals such as U.S. FDA, Japanese health ministry use, and ministry of Health of China in 2000 is also just
Formula is approved as food additives；
(5) insulation stands 2~4h of clarification；
(6) the dilute liquid glucose of molasses after clarification is stood using the separation of molasses centrifuge, takes supernatant, reach clarification purification effect
Fruit；Further comprise precipitation slag processing step before centrifugation：The upper pellucid syrup pump after clarifying to molasses will be stood to centrifuge
Machine machine is separated, and the precipitation slag in settling tank is handled through decanting machine, the dilute liquid glucose remained in slag is at utmost reclaimed, under can be used as
The allotment water of secondary molasses dilution, reduces water consume, improves sugar overall recovery；
(7) supernatant after separating is by plate type heat exchanger, after supernatant temperature is down into 60 DEG C, with 3-4BV/h stream
Speed is decolourized by macroporous strong-base anion-exchange resin column, and diluted molasses percent of decolourization reaches 85%；Large aperture strong alkali anion exchanges
The positive charged group of resin can be combined with the Anion-adsorption in solution, so as to produce anion exchange effect, and macroreticular resin
Internal hole is more and big, has powerful absorption property, can remove pigment, and applicable elements are extensive, and power of regeneration is strong, very suitable
Decolourized with molasses；
(8) supernatant after above-mentioned decolouring enters in surge tank.Starch Hydrolysis liquid glucose and/or reconcile syrup dilution 30~
40 ° of Bx, add Starch Hydrolysis liquid glucose and/or mediation syrup dilution in proportion in surge tank, obtain mixed sugar liquid；The shallow lake
Powder hydrolysis sugar is the product that starch hydrolyzes through two enzymes method, containing more than 90% sugar；The mediation syrup is production FOS production
The byproduct of product, 50-90% glucose and 10-50% sucrose being mainly contained, both sugar are of light color, content of ashes is low,
It can not only increase fermentable sugar in molasses by adding amylum hydrolysate of the sugar and/or reconciling syrup, can also reduce or disappear
Except molasses colour, grey influence of the fluctuation to production process and product quality that grade, fermentation production rate is improved；Hydrolysis sugar and/or mediation
When the addition of syrup is total sugar amount 35-45% or so, yeast yield increase about 9.0%, but after addition continues increasing, because
Trace element and mineral matter in molasses are diluted, and content is reduced, and the production and synthesis for causing yeast are affected, under yield
Drop.If increasing the content of hydrolysis sugar again needs mineral matters such as appropriate increase sodium, magnesium, potassium, phosphorus, zinc, biotin, thiamine and micro
Element just can guarantee that the normal growth metabolism of yeast, just can guarantee that yield and quality.During process implementing, according to the color of molasses
Value, sulfated ass index situation substitute the addition of sugar (hydrolysis sugar or reconcile syrup) to adjust.The colour of molasses acidifying solution and
Sulfated ass reduces with sugar addition is substituted, and the sulfated ass of acidifying solution is reduced into less than 4.5%, while improve acidifying solution
Fermentability sugared content；
(9) mixed sugar liquid is through 118 DEG C~125 DEG C flash distillation processes, after vaporing away organic acid and other pernicious gases, through board-like
Heat exchanger is cooled to 50 DEG C~60 DEG C and used for fermentation；Liquid glucose through handling well is temporarily deposited in storage tank, selects 50 DEG C~60
DEG C it is the probability for avoiding increasing under normal temperature condition microorganism secondary pollution；
(10) by through the good 2000~3000L of mixed sugar liquid of above-mentioned PROCESS FOR TREATMENT, basic nutrient solution (liquid containing ammonium sulfate, phosphoric acid
The ammonium of hydrogen two, magnesium sulfate, phosphoric acid, potassium dihydrogen phosphate etc.) 200~300L, mineral matter and liquid microelement be (containing sodium, zinc, iron, biology
Element, thiamine, inositol etc.) 50~100ml, 5~10m of process water3Ratio fermented, fermentation temperature be 26~32 DEG C, pH
For 3.8~5.5.Culture is separated to culture yeasts after terminating, extracted, drying light, the low ash content yeast product of acquisition.
It is as shown in table 2 for the processing method parameter of molasses at different levels.
Table 2 handles the method parameter of molasses at different levels
Beneficial effects of the present invention
1st, method provided by the invention is economic and environment-friendly, and the flocculating settling agent of use is without residual monomer, and flocculant consumption is small, wadding
Sludge caused by after heavy will not cause secondary pollution；
2nd, material processing provided by the invention, compared with traditional molasses processing method, the dilute liquid glucose colour of molasses reduces
More than 20%, sulfated ass ＜ 4.5%, reduce more than 10%；
3rd, molasses dilution is decolourized present invention employs large aperture strong-base anion-exchange resin, decolorizing effect
85% can be reached；
4th, of the invention compared with traditional molasses processing method, the colourity of product drops to 1 ° for 4 ° by ＞, and ash content reduces
More than 30%, fermentation production rate improves more than 5%, and yeast product is of light color, ash content is low, has good popularizing application prospect.
Brief description of the drawings
The process chart of Fig. 1 three-levels molasses processing fermentation；
Fig. 2 precipitation capacities with Sodium Polyacrylate addition variation diagram.
Embodiment
Molasses quality condition is as shown in table 3 before implementing the present invention：
The before processing molasses quality of table 3
| Molasses sample | Brix Bx | Total reducing sugar % | Purity % | Colour OD560 | Sulfated ass % |
| Guangxi molasses sample | 84 | 46 | 54.7 | 30769 | 15.04 |
According to molasses classifying rules, the molasses belong to the 3rd class molasses.
Molasses biased sample is taken, is respectively adopted at the molasses of traditional treatment method, conventional decoloration treatment method and the present invention
Reason method, contrast the treatment effect of each method condition.
Embodiment 1
Traditional melassic acid method：100g molasses are diluted to 38Bx, add sulfuric acid or phosphorus acid for adjusting pH to add to 3.5~4.5
Heat is warming up to 80~90 DEG C, staticly settles 4~8h, is separated using molasses seperator, takes supernatant to produce acidifying solution.
Embodiment 2
Conventional decoloration treatment method：100g molasses are diluted to 38Bx, add sulfuric acid or phosphorus acid for adjusting pH to add to 3.5~4.5
Heat is warming up to 80~90 DEG C, adds 1% activated carbon, lasting stirring, staticly settles 4~8h, separated, taken using molasses seperator
Clear liquid produces acidifying solution.
Embodiment 3
Conventional decoloration treatment method：100g molasses are diluted to 38Bx, add sulfuric acid or phosphorus acid for adjusting pH to add to 3.5~4.5
Heat is warming up to 80~90 DEG C, adds 1% atlapulgite, lasting stirring, staticly settles 4~8h, separated, taken using molasses seperator
Supernatant produces acidifying solution.
Embodiment 4
The inventive method 1：100g molasses are diluted to 38Bx, adding sulfuric acid or phosphorus acid for adjusting pH, heating rises to 3.5~4.2
Temperature is to 80~90 DEG C, and adding 0.1 ‰ built-up flocculants, (the weight ratio of Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate is 1:
3), agitator persistently stirs 15min with 200r/min, staticly settles 2~4h, and supernatant liquor is separated using molasses seperator, point
Chaotropic is mixed with 30~40Bx of brix amylum hydrolysate of the sugar or the syrup dilution that reconciles by total reducing sugar accounting 40%, is as acidified
Liquid.
Embodiment 5
The inventive method 2：100g molasses are diluted to 38Bx, adding sulfuric acid or phosphorus acid for adjusting pH, heating rises to 3.5~4.2
Temperature is to 80~90 DEG C, and adding 0.1 ‰ built-up flocculants, (the weight ratio of Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate is 1:
3), agitator persistently stirs 15min with 200r/min, staticly settles 2~4h, is separated using molasses seperator, and temperature is down to 60
After DEG C, separating liquid is 100ml by ion column, ion column volume with 300ml/h speed, and clear liquid is acidifying solution.
Embodiment 6
The inventive method 3：100g molasses are diluted to 38Bx, adding sulfuric acid or phosphorus acid for adjusting pH, heating rises to 3.5~4.2
Temperature is to 80~90 DEG C, and adding 0.1 ‰ built-up flocculants, (the weight ratio of Potassium aluminum sulfate dodecahydrate (alum) and Sodium Polyacrylate is 1:
3), agitator persistently stirs 15min with 200r/min, staticly settles 2~4h, is separated using molasses seperator, and temperature is down to 60
After DEG C, for separating liquid with 300ml/h speed by ion column, ion column volume is 100ml, clear liquid and 30~40Bx of brix shallow lake
Powder hydrolysis sugar or the syrup dilution that reconciles are mixed by total reducing sugar accounting 40%, as acidifying solution.
Each 2ml of acidifying solution made from above-mentioned each scheme is taken, is settled to 100ml respectively.By dilute solution be placed in wavelength X=
Absorptance OD is measured at 560nm.Percent of decolourization calculates as follows：
Percent of decolourization=(sample OD after before processing sample OD- processing) ÷ before processing sample OD × 100%
As a result it is listed in table 4.
Comparative result of the table 4 Jing Guo embodiment 1-6 processing methods
As can be seen from Table 4, the inventive method 1-3 uses the processing method of built-up flocculant adsorption precipitation, especially
It is inventive method 2-3 increase resin decolorization steps, the acidifying solution absorbance handled under Detection wavelength 560nm through this method is obvious
Reduce, reflect coloring matter in molasses dilution and be efficiently removed.The removal of coloring matter, it will also be given up to reducing subsequent fermentation
COD in liquidCrConcentration has remarkable effect.The inventive method 1 and 3 adds replacement sugared (hydrolysis sugar or tune on the basis of molasses dilution
And syrup), because the sugared self color of replacement is shallow, ash content is low, a certain amount of mashed up use advantageously reduces the colour of former dilute liquid glucose
And content of ashes.As can also be seen from Table 4, activated carbon or atlapulgite are increased in common method as adsorption decolouriser, one
Determine that the coloring matter in liquid glucose can be removed in degree；And dosage is bigger, decolorizing effect is more notable, but for comprehensive cost performance, this
Two kinds of decolorising agent dosages are more, cost is high, and harsh using (containing regenerative process) practical operation difficulty, condition in production, are unfavorable for producing
Industry amplification is promoted.It can be seen that method of the invention, economic and practical is preferable, avoids molasses dilution as far as possible in processing procedure
Nutrient component damages and inactivation；Adsorption precipitant used meets " three nothings " requirement of the nontoxic noresidue of food processing, no
Environmental pollution can be caused, meets green clean manufacturing objective；Impurity content is low in acidifying solution after processing, fermenting can utilize effectively
Composition is high, meets the ingredient requirement higher to fermentation quality requirement.
The product quality indicator correction data table of table 5
| Product quality | Colourity ° | Ash content % | Fermentation production rate amplification % |
| Conventional method | ＞ 4 | 8.12 | — |
| The inventive method 3 | 1 | 5.01 | 5.1 |
As can be seen from Table 5,1 ° is dropped to for 4 ° by ＞ using the colourity of product after the above method, ash content reduces
38%, fermentation production rate improves more than 5%.Yeast product is of light color, ash content is low, is suitably applied and requires higher milk to color
Food, nutritional health food and the skin care item such as powder, Yoghourt fermentation product, yeast milk piece, yeast albumen powder, yeast shield cosmetics
Requirement.
Embodiment 7
The present embodiment investigates change of the precipitation capacity with built-up flocculant addition.Wait to clarify through Overheating Treatment in every 200ml
Diluted molasses in by gradient add built-up flocculant, precipitation capacity such as Fig. 2 is obtained after being separated using centrifuge：
As can be seen from Figure 2：After adding built-up flocculant, precipitation capacity substantially increases in diluted molasses, when addition reaches
After 0.1 ‰ (w/v), precipitation capacity incrementss slow down, therefore consider from optimum efficiency, diluted molasses addition flocculation after heat treatment
The amount of agent is set as 0.1 ‰ (w/v).
Embodiment 8
The present embodiment is that amylum hydrolysate of the sugar is investigated under conditions of embodiment 6 and reconciles sugared adding proportion to acidifying solution quality
With the influence of fermentation production rate.As a result as shown in table 6 and table 7.
Influence of the amylum hydrolysate of the sugar adding proportion of table 6 to acidifying solution quality and fermentation production rate
Influence of the mediation syrup adding proportion of table 7 to acidifying solution quality and fermentation production rate
Claims (10)
- A kind of 1. method for producing light, low ash content yeast, it is characterised in that the described method comprises the following steps：(1) molasses are dilute Release；(2) acid adjustment；(3) heating ventilation；(4) clarification is stood；(5) centrifuge, take supernatant；(6) flash distillation sterilizing；(7) ferment； (8) refine, obtain yeast.
- 2. the method according to claim 1 for producing light, low ash content yeast, it is characterised in that methods described also includes： In step (5), amylum hydrolysate of the sugar and/or mediation syrup are added into obtained supernatant.
- 3. the method according to claim 1 for producing light, low ash content yeast, it is characterised in that methods described also includes The addition flocculating settling agent before step (3) carries out step (4) afterwards.
- 4. the method according to claim 3 for producing light, low ash content yeast, it is characterised in that the flocculating settling agent It is 1-2 by weight for Potassium aluminum sulfate dodecahydrate and Sodium Polyacrylate:The built-up flocculant that 1-3 compoundings form.
- 5. the method according to claim 3 for producing light, low ash content yeast, it is characterised in that the flocculating settling agent Addition be concentration expressed in percentage by volume 0.01-0.2 ‰.
- 6. the method according to claim 3 for producing light, low ash content yeast, it is characterised in that methods described also includes, Before step (5) carries out step (6) afterwards, enter after carrying out ion-exchange resin decolorization processing in surge tank, then to surge tank Middle addition amylum hydrolysate of the sugar and/or the syrup that reconciles.
- 7. the method for light, the low ash content yeast of production according to claim 2 or 6, it is characterised in that the addition starch The addition of hydrolysis sugar and/or mediation syrup is the 35-45% of total sugar amount.
- 8. the method for light, the low ash content yeast of production according to claim 2 or 6, it is characterised in that the Starch Hydrolysis Sugar is sugar content>90% glucidtemns；The mediation syrup includes 50-90% glucose and 10-50% sucrose.
- 9. the method according to claim 1 for producing light, low ash content yeast, it is characterised in that in step (1), sugar Honey is diluted to 30~40 ° of Bx.
- 10. the method according to claim 1 for producing light, low ash content yeast, it is characterised in that in step (2), adjust PH to 3.5~4.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710951916.9A CN107641603A (en) | 2017-10-13 | 2017-10-13 | A kind of method for producing light, low ash content yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710951916.9A CN107641603A (en) | 2017-10-13 | 2017-10-13 | A kind of method for producing light, low ash content yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107641603A true CN107641603A (en) | 2018-01-30 |
Family
ID=61113701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710951916.9A Pending CN107641603A (en) | 2017-10-13 | 2017-10-13 | A kind of method for producing light, low ash content yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107641603A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284693A (en) * | 2008-05-26 | 2008-10-15 | 董超平 | Natural organic polymer composite flocculant, method for preparing the same and applications |
| CN102660605A (en) * | 2012-04-28 | 2012-09-12 | 中粮生物化学（安徽）股份有限公司 | Method for manufacturing sugar through wet process and method for preparing lysine |
| CN103820520A (en) * | 2014-03-06 | 2014-05-28 | 浙江皇冠科技有限公司 | High-yield natural astaxanthin fermentation method |
-
2017
- 2017-10-13 CN CN201710951916.9A patent/CN107641603A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284693A (en) * | 2008-05-26 | 2008-10-15 | 董超平 | Natural organic polymer composite flocculant, method for preparing the same and applications |
| CN102660605A (en) * | 2012-04-28 | 2012-09-12 | 中粮生物化学（安徽）股份有限公司 | Method for manufacturing sugar through wet process and method for preparing lysine |
| CN103820520A (en) * | 2014-03-06 | 2014-05-28 | 浙江皇冠科技有限公司 | High-yield natural astaxanthin fermentation method |
Non-Patent Citations (3)
| Title |
|---|
| 林波等: "天然高分子絮凝剂分离澄清甘蔗糖蜜", 《食品研究与开发》 * |
| 田原等: "大孔离子交换树脂对大豆糖蜜脱色效果的研究", 《中国油脂》 * |
| 黎海彬等: "化学絮凝分离净化甘蔗废糖蜜研究", 《韶关学院学报 自然科学版》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101899536B (en) | Application of bioflocculant synthesized by bacillus licheniformis in refining sugar | |
| CN102173488B (en) | Method for recycling rice milk | |
| CN103695489B (en) | A kind of arginine process for refining | |
| CN101120766B (en) | Monosodium glutamate green manufacturing technology | |
| US20120009641A1 (en) | Method for producing clarified juice, packagible juice, ethanol and sugar from sugarcane and system thereof | |
| WO2014078924A1 (en) | Process and equipment for multistage, continuous fermentation, with ferment recovery, reactivation and recycling, for producing wines with a high alcohol content | |
| CN103695487B (en) | A kind of fermentable produces arginine technique | |
| CN103667381B (en) | A kind of method improving yield of arginine | |
| US20220240544A1 (en) | Method to produce high protein residuals | |
| CN103409471B (en) | Method for rapidly clearing high-concentration molasses | |
| CN106399388B (en) | method for producing alcohol by using clear liquid of distiller's grains and corn | |
| FI97149B (en) | Method for clarifying vinasse | |
| CN101319232B (en) | Method for preparing ethyl alcohol with corn fermentation | |
| CN101823953B (en) | Method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization | |
| CN107641603A (en) | A kind of method for producing light, low ash content yeast | |
| CN103320545B (en) | Method for removing acidic flocculate in white sugar | |
| CN102211840A (en) | Resource utilization device and method of restaurant wastewater | |
| CN103695490A (en) | High-purity arginine production process | |
| CN102372645A (en) | Glutamic acid fermentation and extraction process | |
| CN103695488B (en) | A kind of arginine preparation method | |
| CN1218111A (en) | Production of sugar from corn starch and glutamic acid fermentation | |
| CN109797106A (en) | A kind of novel two stages autotrophy-is photosynthetic and supports the method for improving chlorella lipid | |
| CN1284857C (en) | Clean production process for producing alcohol by fermentation of saccharate crop clean juice | |
| CN105861568A (en) | Method for fermenting alcohol by using molasses without additional acid | |
| CN111793574A (en) | Microbial biomass production method without waste nutrients |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180130 |