CN105861568A - Method for fermenting alcohol by using molasses without additional acid - Google Patents
Method for fermenting alcohol by using molasses without additional acid Download PDFInfo
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- CN105861568A CN105861568A CN201610439936.3A CN201610439936A CN105861568A CN 105861568 A CN105861568 A CN 105861568A CN 201610439936 A CN201610439936 A CN 201610439936A CN 105861568 A CN105861568 A CN 105861568A
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- Prior art keywords
- molasses
- fermentation
- alcohol
- tank
- distillation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
Abstract
The invention belongs to the technical field of sugaring comprehensive utilization and biological fermentation and discloses a method for fermenting alcohol by using molasses without additional acid. The method comprises the following steps: diluting molasses and heating for sterilization; adding diammonium hydrogen phosphate and sodium alginate into one part of the material, introducing sterile compressed air, sufficiently stirring and standing for clarification, and removing the precipitate at the tank bottom; then, adding lysozyme, introducing sterile compressed air and sufficiently stirring; cooling and diluting, and continuously feeding a culture yeast into a seeding tank; after the tank is full, enabling the culture yeast to continuously flow into a main fermentation tank; diluting the other part of the material as a fermentation culture solution; continuously feeding into a fermentation tank till fermentation maturity; and finally, feeding the fermented mature mash into an alcohol distillation tower for distillation to obtain alcohol. In the invention, the lysozyme replaces sulfuric acid for sterilization, the molasses can be heated through alcohol distillation and waste hot water dilution, the yeast reproduction and fermentation are promoted, and the alcohol content of fermentation is increased from 10% (v/v) to about 11.0% (v/v).
Description
Technical field
The invention belongs to sugaring comprehensive utilization and technical field of biological fermentation, be specifically related to a kind of molasses without additional
The method of acid fermentation ethanol.
Background technology
Molasses are a kind of side-products of Caulis Sacchari sinensis or beet-sugar factory, and also known as molasses, brix is generally 80～90Bx,
Constituent mainly has the nutritions such as sucrose, reducing sugar, aminoacid, vitamin and trace element, is to send out
Ferment produces the very good material of the fermented product such as ethanol, yeast, aminoacid, in molasses possibly together with inorganic salt ash,
The most available material of the yeast such as colloid, in recent years, sugar refinery sugar rate to improve and improves white sugar product
Quality, many enterprises tend to use the sugar refining technologies such as highly basic, high-sulfur, high ash, and side-product molasses purity drops
Low, the non-sugar impurities content such as ash, colloid constantly raises.
Domestic molasses-spirit produces mainly by adding sulphuric acid acidifying molasses, and bacteria growing inhibiting is bred, and clear
Clear sedimentation small part colloid and ash, constantly decline due to molasses purity in recent years, and sulfuric acid dosage has constantly liter
High trend, ton ethanol consumption sulfuric acid amount is increased to 50～60 kilograms by 20～30 kilograms, sulphuric acid suppression miscellaneous bacteria
Meanwhile, also can the suppression breeding of yeast in various degree and fermentation, affect liquor ratio of raw material.A large amount of uses are dense
Sulphuric acid, not only causes the biggest harm, and easily causes equipment corrosion and dirty, also can increase environment
Slops treatment difficulty, ethanol environmental protection fund input increases.
Molasses fermented alcohol effluent generation amount is big, often produces 1 ton of ethanol and can produce 11～13 tons of waste liquids, waste liquid
Middle Organic substance and inorganic ash content content are high, and liquid waste processing difficulty is big, the wherein nothing with sulfate ion as representative
Machine salt is maximum on the impact of liquid waste processing.During alcohol effluent anaerobic treatment, sulphuric acid can suppress methane backeria
Growth metabolism, affect biogas generate；In waste liquid evaporating concentration process, calcium sulfate can make evaporator fouling
And seriously corroded；During the burning of ethanol concentrating spent liquor, the easy boiler coke of existence of sulphuric acid and corrode boiler；
In alcohol effluent agriculture fills or make the technique in liquid fertilizer, solid-state fertilizer fertilising farmland, life-time service easily causes
Soil compaction.
Due to the adverse effects adding sulphuric acid a large amount of in alcohol fermentation, exploitation will become without additional acid fermentation technique
The developing direction of Alcohol Production technology, the molasses-spirit of research and development is without acid fermentation in current industry, mainly from
Kill antibacterial to start with, use the means such as antibacterial and compoiste fermented enzyme, but in molasses increasing colloid with
Ash grades material, can suppress breeding and the fermentation of yeast, and yeast in alcohol fermentation and antibacterial are with growth vigor
Competing each other, when cannot form growth vigor when yeast is suppressed, antibacterial will produce drug resistance, overcome
The suppression of antibacterial, is formed yeast growth advantage, causes antibacterial to infect, have a strong impact on anacidity ferment effect.
Therefore research anacidity fermentation technique, except research bacteriostatic technology, prior molasses to be studied without
Acid clarification and sterilization technology, kill antibacterial from raw material source economy, and get rid of the ash of suppression yeast propagation
With materials such as colloids, such anacidity fermentation technique can improve fermenting speed, extension device service life,
Reduce the recycling of alcohol effluent level of pollution, beneficially alcohol effluent, meet national energy ethanol and subtract
The industrial policy of row's consumption reduction.
Summary of the invention
In place of solving the shortcoming and defect of above prior art, it is an object of the invention to provide a kind of sugar
Honey is without the method for additional acid fermentation ethanol.
The object of the invention is achieved through the following technical solutions:
A kind of molasses, without the method for additional acid fermentation ethanol, comprise the steps:
(1) in thinning tank, molasses with water is diluted to 50～55Bx, and is heated to 85～95 DEG C of sterilizings；
(2) material of a part of step (1) being flowed into settling tank, adding mass fraction is 0.3%～0.5%
Diammonium phosphate and concentration be 5～10ppm sodium alginates, logical aseptic compressed air stands clear after being sufficiently stirred for
Clearly, sediment on tank bottom is got rid of；
(3) in the material of step (2), the lysozyme that concentration is 10～20ppm is added, logical aseptic compression sky
Gas is sufficiently stirred for, and is diluted to 18～20Bx after cooling, and stream adds seed tank culture yeast, warp after canful continuously
Charge tube continuously flows into main fermentation tank；
(4) it is diluted to 40～45Bx after the material cooling of another part step (1), as fermentation culture,
Continuously stream adds main fermentation tank, flows into follow-up continuous fermentation tank successively until fermenting-ripening after main fermentation tank canful；
(5) fermenting-ripening mash enters alcohol distillation tower system, and after distillation, tower top obtains ethanol and distills cool solidifying
Water, obtains alcohol distilled wastewater at the bottom of tower.
Preferably, the distillation produced during the water described in step (1) refers to step (5) alcohol distillation
Cool solidifying water, the temperature distilling cool solidifying water is 60～75 DEG C；Described heating refers to use step (5) ethanol to steam
The alcohol distilled wastewater produced during evaporating heats, and the temperature of alcohol distilled wastewater is 100～106 DEG C.
It is highly preferred that the dilution described in step (1) and heating refer to directly use step (5) ethanol to steam
The alcohol distilled wastewater produced during evaporating is diluted simultaneously and heats, and the temperature of alcohol distilled wastewater is
100～106 DEG C.
Preferably, the sterilization time described in step (1) is 1～2h.
Preferably, step (2) the described time standing clarification is 0.5～1h.
Preferably, the cooling described in step (3) and step (4) refers to be cooled to less than 30 DEG C.
Relative to prior art, the invention have the advantages that and beneficial effect:
(1) alcohol distillation waste heat sterilizing molasses are utilized: molasses without outer acid adding sterilization treatment, are utilized by the present invention
Alcohol distillation spent hot water dilutes heating molasses, both can kill major part bacterial vegetative cells, and compare high-temperature boiling
Sterilization, can guarantee that again molasses are not converted into caramel, and makes full use of the waste heat of Alcohol Production, save the energy
Consume；
(2) molasses are clarified without outer acid adding: colloid and ash in molasses substantially suppress yeast propagation and fermentation,
The present invention adds sodium alginate, can generate calcium alginate gel with the calcium salt ash fractional precipitation in molasses, inhale
Colloid in attached molasses and float, clarify and get rid of to materials such as the ash of fermentation inhibitory and colloids in molasses,
Breeding and fermentation with beneficially yeast；Molasses add diammonium phosphate, supplement the N needed for yeast growth,
The nutrients such as P, suppress antibacterial with yeast growth advantage, it is also possible to improve fermenting speed；
(3) lysozyme substitutes sulphuric acid sterilization: the method for the present invention adds lysozyme suppression antibacterial, the most not outside
Add sulphuric acid, eliminate sulphuric acid to yeast propagation and the suppression of fermentation, environment will not be caused bigger pollution,
Reduce equipment corrosion and dirty；Lysozyme can kill the antibacterial of alcohol fermentation, and do not have killing action to yeast,
The most not being used in alcohol fermentation, the present invention studies and finds that the lysozyme of trace just can play the work that well sterilizes
With, the Bacteria cognition after killing is decomposed into aminoacid, may advantageously facilitate yeast propagation and fermentation, can make to send out
Ferment is brought up to 11.0% (v/v) left and right containing wine part by 10% (v/v).
Accompanying drawing explanation
Fig. 1 is a kind of molasses process chart without the method for additional acid fermentation ethanol of the embodiment of the present invention 1.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but the embodiment party of the present invention
Formula is not limited to this.
Embodiment 1
A kind of molasses of the present embodiment are without the method for additional acid fermentation ethanol, and its process chart is as shown in Figure 1.
Specifically comprise the following steps that
(1) in thinning tank, molasses are distilled cool solidifying water (68 DEG C) and is diluted to 53Bx, then steam with ethanol
Evaporate waste water (temperature 103 DEG C) and be heated to 90 DEG C of sterilizing 1h；
(2) material of a part of step (1) being flowed into settling tank, adding mass fraction is the phosphoric acid of 0.4%
Hydrogen diammonium and concentration are 8ppm sodium alginate, and logical aseptic compressed air stands clarification 1h, row after being sufficiently stirred for
Except sediment on tank bottom；
(3) adding concentration in the material of step (2) is the lysozyme of 15ppm, logical aseptic compressed air
It is sufficiently stirred for, in continuous diluter, is diluted to 19Bx after being cooled to 30 DEG C by condenser, flows addition continuously
Seed tank culture yeast, continuously flows into main fermentation tank through charge tube after canful；
(4) the dilutest after the material of another part step (1) thinning tank is cooled to 30 DEG C by condenser
Releasing and be diluted to 43Bx in device, as fermentation culture, stream adds main fermentation tank continuously, after main fermentation tank canful
Flow into follow-up continuous fermentation tank successively until fermenting-ripening；
(5) fermenting-ripening mash sequentially enters alcohol distillation tower system and (generally includes primary distillation tower, row's aldehyde tower and essence
Evaporate tower), after each distillation tower top alcohol steam is condensed by 2～4 grades of condensers, enter next stage distillation column or return
Flowing this tower, finished product ethanol extracts at tower top.Take each distillation column first order Water in Condenser (distill cool solidifying water,
68 DEG C) as the molasses thinned water of step (1)；Take alcohol waste water at the bottom of rectifying column (alcohol distilled wastewater,
103 DEG C) as the molasses water for heating of step (1).
Being computed, the present embodiment fermentation liquid contains wine part by the 10% of traditional fermentation methods (sulphuric acid sterilization fermentation)
(v/v) 11.0% (v/v) is brought up to.
Embodiment 2
(1) in thinning tank, molasses alcohol distilled wastewater (temperature 106 DEG C) is diluted to 55Bx, and
Controlling diluent temperature is 95 DEG C of sterilizing 1h；
(2) material of a part of step (1) being flowed into settling tank, adding mass fraction is the phosphoric acid of 0.5%
Hydrogen diammonium and concentration are 10ppm sodium alginate, and logical aseptic compressed air stands clarification 1h, row after being sufficiently stirred for
Except sediment on tank bottom；
(3) adding concentration in the material of step (2) is the lysozyme of 20ppm, logical aseptic compressed air
It is sufficiently stirred for, in continuous diluter, is diluted to 20Bx after being cooled to 30 DEG C by condenser, flows addition continuously
Seed tank culture yeast, continuously flows into main fermentation tank through charge tube after canful；
(4) after the material of another part step (1) is cooled to 30 DEG C by condenser in continuous diluter
Being diluted to 45Bx, as fermentation culture, stream adds main fermentation tank continuously, flows successively after main fermentation tank canful
Enter follow-up continuous fermentation tank until fermenting-ripening；
(5) fermenting-ripening mash sequentially enters alcohol distillation tower system and (generally includes primary distillation tower, row's aldehyde tower and essence
Evaporate tower) each tower, after each distillation tower top alcohol steam is condensed by 2～4 grades of condensers, enter next stage distillation
Tower or this tower that refluxes, finished product ethanol extracts at tower top.Take alcohol waste water at the bottom of rectifying column (alcohol distilled wastewater,
106 DEG C) as step (1) molasses dilute and water for heating.
Being computed, the present embodiment fermentation liquid contains wine part by the 10% of traditional fermentation methods (sulphuric acid sterilization fermentation)
(v/v) 11.5% (v/v) is brought up to.
Embodiment 3
(1) in thinning tank, molasses are distilled cool solidifying water (60 DEG C) and is diluted to 50Bx, then steam with ethanol
Evaporate waste water (temperature 100 DEG C) and be heated to 85 DEG C of sterilizing 1h；
(2) material of a part of step (1) being flowed into settling tank, adding mass fraction is the phosphoric acid of 0.3%
Hydrogen diammonium and concentration are 5ppm sodium alginate, and logical aseptic compressed air stands clarification 1h, row after being sufficiently stirred for
Except sediment on tank bottom；
(3) adding concentration in the material of step (2) is the lysozyme of 10ppm, logical aseptic compressed air
It is sufficiently stirred for, in continuous diluter, is diluted to 18Bx after being cooled to 30 DEG C by condenser, flows addition continuously
Seed tank culture yeast, continuously flows into main fermentation tank through charge tube after canful；
(4) after the material of another part step (1) is cooled to 30 DEG C by condenser in continuous diluter
Being diluted to 40Bx, as fermentation culture, stream adds main fermentation tank continuously, flows successively after main fermentation tank canful
Enter follow-up continuous fermentation tank until fermenting-ripening；
(5) fermenting-ripening mash sequentially enters alcohol distillation tower system and (generally includes primary distillation tower, row's aldehyde tower and essence
Evaporate tower) each tower, after each distillation tower top alcohol steam is condensed by 2～4 grades of condensers, enter next stage distillation
Tower or this tower that refluxes, finished product ethanol extracts at tower top.Take each distillation column first order Water in Condenser and (distill cool
Solidifying water, 60 DEG C) as the molasses thinned water of step (1)；Take alcohol waste water (alcohol distillation at the bottom of rectifying column
Waste water, 100 DEG C) as the molasses water for heating of step (1).
Being computed, the present embodiment fermentation liquid contains wine part by the 10% of traditional fermentation methods (sulphuric acid sterilization fermentation)
(v/v) 10.5% (v/v) is brought up to.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-mentioned reality
Execute the restriction of example, the change made under other any spirit without departing from the present invention and principle, modification,
Substitute, combine, simplify, all should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (6)
1. molasses are without the method for additional acid fermentation ethanol, it is characterised in that comprise the steps:
(1) in thinning tank, molasses with water is diluted to 50～55Bx, and is heated to 85～95 DEG C of sterilizings；
(2) material of a part of step (1) being flowed into settling tank, adding mass fraction is 0.3%～0.5%
Diammonium phosphate and concentration be 5～10ppm sodium alginates, logical aseptic compressed air stands clear after being sufficiently stirred for
Clearly, sediment on tank bottom is got rid of；
(3) in the material of step (2), the lysozyme that concentration is 10～20ppm is added, logical aseptic compression sky
Gas is sufficiently stirred for, and is diluted to 18～20Bx after cooling, and stream adds seed tank culture yeast, warp after canful continuously
Charge tube continuously flows into main fermentation tank；
(4) it is diluted to 40～45Bx after the material cooling of another part step (1), as fermentation culture,
Continuously stream adds main fermentation tank, flows into follow-up continuous fermentation tank successively until fermenting-ripening after main fermentation tank canful；
(5) fermenting-ripening mash enters alcohol distillation tower system, and after distillation, tower top obtains ethanol and distills cool solidifying
Water, obtains alcohol distilled wastewater at the bottom of tower.
A kind of molasses the most according to claim 1 are without the method for additional acid fermentation ethanol, it is characterised in that:
The cool solidifying water of distillation that water described in step (1) produces during referring to step (5) alcohol distillation, distillation
The temperature of cool solidifying water is 60～75 DEG C；Described heating produces during referring to use step (5) alcohol distillation
Alcohol distilled wastewater heat, the temperature of alcohol distilled wastewater is 100～106 DEG C.
A kind of molasses the most according to claim 1 are without the method for additional acid fermentation ethanol, it is characterised in that:
Dilution described in step (1) and heating produce during referring to directly use step (5) alcohol distillation
Alcohol distilled wastewater is diluted simultaneously and heats, and the temperature of alcohol distilled wastewater is 100～106 DEG C.
A kind of molasses the most according to claim 1 are without the method for additional acid fermentation ethanol, it is characterised in that:
Sterilization time described in step (1) is 1～2h.
A kind of molasses the most according to claim 1 are without the method for additional acid fermentation ethanol, it is characterised in that:
Step (2) the described time standing clarification is 0.5～1h.
A kind of molasses the most according to claim 1 are without the method for additional acid fermentation ethanol, it is characterised in that:
Cooling described in step (3) and step (4) refers to be cooled to less than 30 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610439936.3A CN105861568A (en) | 2016-06-17 | 2016-06-17 | Method for fermenting alcohol by using molasses without additional acid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610439936.3A CN105861568A (en) | 2016-06-17 | 2016-06-17 | Method for fermenting alcohol by using molasses without additional acid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107299117A (en) * | 2016-04-15 | 2017-10-27 | 苏州昆蓝生物科技有限公司 | A kind of method of use alcohol produced from molasses |
| CN108642094A (en) * | 2018-08-17 | 2018-10-12 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method of the antibacterial molasses alcohol fermentation of biology |
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| CN103409471A (en) * | 2013-08-28 | 2013-11-27 | 广州甘蔗糖业研究所 | Method for rapidly clearing high-concentration molasses |
| CN105219805A (en) * | 2015-11-10 | 2016-01-06 | 广州甘蔗糖业研究所 | A kind of method of high density molasses high-efficiency fermenting alcohol |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107299117A (en) * | 2016-04-15 | 2017-10-27 | 苏州昆蓝生物科技有限公司 | A kind of method of use alcohol produced from molasses |
| CN107299117B (en) * | 2016-04-15 | 2020-07-28 | 苏州昆蓝生物科技有限公司 | Method for producing alcohol by using molasses |
| CN108642094A (en) * | 2018-08-17 | 2018-10-12 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method of the antibacterial molasses alcohol fermentation of biology |
| CN108642094B (en) * | 2018-08-17 | 2024-03-12 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Biological antibacterial molasses alcohol fermentation method |
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