CN107637599A - A kind of method for preventing and treating cucumber root-knot nematode - Google Patents
A kind of method for preventing and treating cucumber root-knot nematode Download PDFInfo
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- CN107637599A CN107637599A CN201711104231.7A CN201711104231A CN107637599A CN 107637599 A CN107637599 A CN 107637599A CN 201711104231 A CN201711104231 A CN 201711104231A CN 107637599 A CN107637599 A CN 107637599A
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- fluopyram
- root
- knot nematode
- methyl jasmonate
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Abstract
The present invention relates to a kind of method for preventing and treating cucumber root-knot nematode, use by fluopyram and methyl jasmonate according to weight ratio as 1 20:The pesticidal preparations of 1 compounding composition, are diluted with water by the 100g/ mus of effective ingredient consumption in preparation 60 and to cucumber are sprayed or poured, continuous dispenser 23 times, 57 days dispenser intervals.Synergistic of the present invention, can effectively preventing root knot nematode disease, advantageously reduce the usage amount of drug cost and chemical pesticide, delay the resistance of root-knot nematode, extend the life cycle of fluopyram.
Description
Technical field
The present invention relates to agricultural technology field, more particularly to a kind of method for preventing and treating cucumber root-knot nematode.
Background technology
Plant nematode mainly includes：Meloidogyne (Meloidogyne), kidney shape turbatrix
(Rotylenchulus), cyst roundworm (Heterodera (Heterodera)), root spot nematode (Pratylenchus
(Pratykenchus)), root-knot nematode occurs universal in China, and harm is serious.Plant can be greatly decreased in the direct feeding of nematode
To nutrition and the absorption of moisture, nematode Plant species eaten causes wound, returns phytopathogen invasion and provides convenience.Nematode at present
Preventing and treating alternative is few, agriculturally prevents and treats nematode and concentrates on invasion and attack of the prevention nematode to plant substantially.With eelworm harm
Getting worse, it is badly in need of developing new, efficient, low-toxin farm chemicals preparation.
Fluopyram, English name：Fluopyram, it is the new pyridine base ethyl benzamide of Beyer Co., Ltd's exploitation
Series bactericidal agent, pesticidal preparations, act on mitochondrial respiratory chain, suppress the activity of succinate dehydrogenase (complexⅱ) so as to block
Electron transmission, cause the energy requirement that body tissue can not be provided, and then kill controlling object or suppress it and grow.
Methyl jasmonate (Methyl Jasmonate, abbreviation JA_Me), it is the derivative of a kind of aliphatic acid, is present in height
Deng the endogenous growth regulators matter in plant.Jasmonic is a kind of hormone, is prevalent in various plants, is had extensive
Physiological function, the endogenous growth regulators matter being presently believed to be in higher plant body, plant is participated in as endogenous signal molecule
Degeneration-resistant reaction under the conditions of mechanical wounding, pest and disease damage, arid, high temperature, low temperature etc..
In the real process of agricultural production, controlling disease worm is easiest to generate the problem that the generation of disease and insect resistance.
And the farm chemical ingredients mixture of different mechanism of action is used, it is the common methods for preventing and treating resistance pest and disease damage.Screened by formulation of pesticide,
Show that mixture has synergistic function in certain ratio range between heterogeneity, it is possible to increase actual prevention effect, be to control
Manage the important means of pest and disease damage resistance.
At present, the control of nematode medicament using fluopyram and methyl jasmonate as active ingredient is also without related report
Road.
The content of the invention
It is an object of the invention to provide a kind of method for preventing and treating cucumber root-knot nematode, to improve the preventing and treating of root-knot nematode effect
Fruit, delay resistance of the root-knot nematode to single medicament, reduce cost accounting, extend the life cycle of fluopyram.
The technical solution used in the present invention is:A kind of method for preventing and treating cucumber root-knot nematode, using by fluopyram
According to weight ratio it is 1-20 with methyl jasmonate:The pesticidal preparations of 1 compounding composition, by effective ingredient consumption 60-100g/ in preparation
Mu is diluted with water and to cucumber is sprayed or poured, continuous dispenser 2-3 times, 5-7 days dispenser interval.
Preferably, fluopyram and the weight of methyl jasmonate ratio are 5-10:1.
Further, fluopyram and the weight of methyl jasmonate ratio are 8:1.
Described pesticidal preparations, percentage by weight of the fluopyram in pesticidal preparations are 0.5-50%, jasmonic first
Percentage by weight of the ester in pesticidal preparations is 0.1-5%.
Described pesticidal preparations are configured to spray or carry out soil or suspending agent, the water and milk of seed treatment after being available for dilution
Agent, microemulsion, wettable powder, water dispersible granules, it may be made as granule and directly apply.Preferred dosage form is suspending agent, particle
Agent.
Described pesticidal preparations be prepared into the adjuvant that suspending agent or granule add include solvent, filler, binding agent, point
Powder, wetting agent, thickener, sustained release agent, stabilizer or it is other be beneficial to active component in the formulation stablize and drug effect play
Material, all it is to be commonly used in agriculture field preparation or allow the various composition used.
The present invention has advantages below and notable technique effect：
(1) active ingredient methyl jasmonate and fluopyram Synergistic of the invention, are used with, energy in the reasonable scope
The amount of application of each single dose is reduced, advantageously reduces the usage amount of drug cost and chemical pesticide.
(2) active ingredient methyl jasmonate of the present invention is different with the mechanism of action of fluopyram, and methyl jasmonate can also
The resistance of aggrieved diseased plant is improved, delays the resistance of root-knot nematode, extends the life cycle of fluopyram.
Embodiment
Technical scheme and beneficial effect are described further with reference to embodiments, of the present invention hundred
Ratio is divided to be weight percentage.
First, indoor joint toxicity measuring test
Embodiment 1：Fluopyram compounds the toxicity test to cucumber root-knot nematode with methyl jasmonate
Subjects：Cucumber root-knot nematode (Meloidogyne incognita)
Test method：Pot-culture method, the pieces of an egg sterile water wash of picking root-knot nematode, is placed in culture dish from cucumber root
On wet filter paper, 20ml sterilized waters are added, are hatched at 20 DEG C -25 DEG C, age consistent second instar larvae is obtained, is diluted to sterilized water
400/ml.Second instar larvae is inoculated with the leaf phase of cucumber two, using root injection method, takes 5ml nematodes inoculation liquid to inject rhizosphere soil
It is interior, 24h soil conservations 70%-80% humidity after inoculation.It is standby after cucumber inoculation root-knot nematode.
Mother liquor is made into after active ingredient is dissolved on request, then carries out pouring root with purified water gradient dilution to suitable concentration
Processing.Cucumber seedling is placed in phjytotron after chemicals treatment, then 25-30 DEG C, 12h alternation of light and darkness, relative humidity 35-
30d is cultivated under the conditions of 50%.Each to repeat the basin of random searching 4, each processing amounts to 16 basin cucumber of investigation.Grade scale is by China
People's republic's national standard《Pesticide field efficacy medicine test criterion (one)》, severity Scaling standard.
Diseased plant stage division is as follows：
0 grade：Root system is without insect gall；
1 grade：Root system has a small amount of small insect gall；
3 grades：2/3rds root systems are covered with small insect gall；
5 grades：Root system is covered with small insect gall and has secondary insect gall；
7 grades：Root system forms fibrous root group；
Computational methods：
Prevention effect is converted into niqueMin (y), liquor strength (μ g/ml) is converted into logarithm value (x), in terms of least square
Concentration EC in calculating virulence equation and suppressing50, according to the toxicity index and co-toxicity coefficient (CTC) of the abundant method calculating medicaments of Sun Yun.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
A percentage composition+B medicament toxicity index in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
B percentage composition in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
When CTC≤80, then composition shows as antagonism, and as 80 ＜ CTC ＜ 120, then composition shows as being added and made
With when CTC >=120, then composition shows as synergistic effect.
Result of the test is shown in Table 1.
The fluopyram of table 1 compounds the toxicity test to cucumber root-knot nematode with methyl jasmonate
The measurement result of indoor co-toxicity shows that fluopyram compares 1-20 with methyl jasmonate with weight:1 mixture
When, co-toxicity coefficient (CTC) has synergistic function to be all higher than 120.When fluopyram and the weight of methyl jasmonate ratio
8:When 1, CTC 188.9, synergistic effect is more notable, there is fine guiding value in field test.
2nd, embodiment is prepared
Embodiment 1：55% fluopyram methyl jasmonate suspending agent
Formula：Fluopyram 50.0%, methyl jasmonate 5.0%, thermally coupled distillation columns are weighed according to quantity
4.5%, carboxymethyl cellulose 1.2%, glycerine 4%, water complements to 100%.
Preparation method：Each composition is well mixed, ground in a usual manner and/or high speed shear, in water-medium
The high dispersive of formation, stable suspension system, you can 55% fluopyram methyl jasmonate suspending agent is made.
The embodiment prevents and treats cucumber root-knot nematode.55% fluopyram methyl jasmonate suspending agent is pressed into active ingredient
Dosage 80g/ mus are diluted with water leaching ground, continuous dispenser 2 times, 7 days dispenser intervals, the prevention effect of 20 days and 50 days after last medicine
Respectively 86.7%, 92.6%.25% fluopyram suspending agent presses effective ingredient consumption 100g/ mus and 5% methyl jasmonate
Suspending agent presses 250g/ mus, in kind uses, the preventive effect of 20 days and 50 days point after 25% fluopyram suspending agent last medicine
Wei 73.7%, 74.6%；After 5% methyl jasmonate suspending agent last medicine the preventive effect of 20 days and 50 days be respectively 63.8%,
54.2%.Synergistic effect is obvious after fluopyram compounds with methyl jasmonate, and the preventive effect of cucumber root-knot nematode is significantly better than
Single dose.
In summary, pesticidal preparations of the invention have obvious synergistic effect, can effectively control the danger of root-knot nematode
Evil, reduces the dosage of fluopyram, reduces cost, delay nematode resistance to develop, and it is online to be advantageous to extension fluopyram
Worm applies upper life cycle.
Claims (3)
- A kind of 1. method for preventing and treating cucumber root-knot nematode, it is characterised in that using by fluopyram and methyl jasmonate according to Weight ratio is 1-20:The pesticidal preparations of 1 compounding composition, are diluted with water to cucumber by effective ingredient consumption 60-100g/ mus in preparation Ground is sprayed or poured, continuous dispenser 2-3 times, 5-7 days dispenser interval.
- 2. according to the method for claim 1, it is characterised in that fluopyram and the weight of methyl jasmonate ratio are 5- 10:1。
- 3. according to the method for claim 2, it is characterised in that fluopyram and the weight of methyl jasmonate ratio are 8:1.
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| CN107683854A (en) * | 2017-10-24 | 2018-02-13 | 天峨县平昌生态农业有限公司 | A kind of nematicide of containing fluopyram and methyl jasmonate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107683854A (en) * | 2017-10-24 | 2018-02-13 | 天峨县平昌生态农业有限公司 | A kind of nematicide of containing fluopyram and methyl jasmonate |
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