CN107601681A - A kind of composite bacteria agent for repairing metamitron polluted-water - Google Patents
A kind of composite bacteria agent for repairing metamitron polluted-water Download PDFInfo
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- CN107601681A CN107601681A CN201711060798.9A CN201711060798A CN107601681A CN 107601681 A CN107601681 A CN 107601681A CN 201711060798 A CN201711060798 A CN 201711060798A CN 107601681 A CN107601681 A CN 107601681A
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- composite bacteria
- seed liquor
- water
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- metamitron
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- 241000894006 Bacteria Species 0.000 title claims abstract description 42
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- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 27
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- 206010003694 Atrophy Diseases 0.000 claims abstract description 16
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- 229920006380 polyphenylene oxide Polymers 0.000 claims description 10
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- 239000007864 aqueous solution Substances 0.000 claims description 8
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- 238000000034 method Methods 0.000 claims description 8
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- 238000002360 preparation method Methods 0.000 claims description 5
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 238000003828 vacuum filtration Methods 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 239000011686 zinc sulphate Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims 1
- NWFNSTOSIVLCJA-UHFFFAOYSA-L copper;diacetate;hydrate Chemical compound O.[Cu+2].CC([O-])=O.CC([O-])=O NWFNSTOSIVLCJA-UHFFFAOYSA-L 0.000 claims 1
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- FCSLKRVBOXMLOR-UHFFFAOYSA-N N1CCNCC1.C1=CC=CC=C1 Chemical compound N1CCNCC1.C1=CC=CC=C1 FCSLKRVBOXMLOR-UHFFFAOYSA-N 0.000 description 2
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- VMSLCPKYRPDHLN-NRFANRHFSA-N humulone Chemical compound CC(C)CC(=O)C1=C(O)C(CC=C(C)C)=C(O)[C@@](O)(CC=C(C)C)C1=O VMSLCPKYRPDHLN-NRFANRHFSA-N 0.000 description 2
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Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to microbial technology field, discloses a kind of composite bacteria agent for repairing metamitron polluted-water, it is prepared in accordance with the following steps：1）Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and pseudomonas nigrifaciens seed liquor is uniform, then it is inoculated in inorganic salts domestication culture medium, culture obtains composite bacteria liquid；2）Composite bacteria liquid is put into the carrier of twice of mass composite bacteria agent is made.Composite bacteria agent of the present invention can effectively repair metamitron polluted-water, and clearance is high, and environment friendly is strong.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of composite bacteria agent for repairing metamitron polluted-water.
Background technology
Metamitron（metamitron）Chemical name be 4- amino -3- methyl -6- phenyl -1,2,4- triazines -5
(4H) -one, belong to Triazinone selectivity preemergence herbicide, have wide range of applications, mainly absorbed by plant root, then be transported to
In leaf, acted on by suppressing photosynthetic Hill reaction to play herbicide.Single, double cotyledon weeds can be prevented and kill off, are a kind of answer
For preventing and kill off the herbicide of Sugarbeet Fields grass family and broad leaved weed, it is mainly used in dry crop, such as corn, the field of beet crop
Between weeding, a variety of weeds such as Li Longkui, chickweed, white dead nettle, annual bluegrass can also be prevented and treated.
Metamitron agricultural chemicals is lost in environment, will cause serious environmental pollution, is even caused sometimes extremely hazardous
Consequence.The agricultural chemicals in environment is lost to by evaporation, transpiration, is floatd among air, the agricultural chemicals to wave is again by the dust in air
Adsorb.And spread with the wind.Cause the pollution of atmospheric environment.Agricultural chemicals in air, further through rainfall, these agricultural chemicals flow into water again
In, so as to cause the pollution of water environment, to people, animal, particularly aquatile（Such as fish, shrimp）Damage.Meanwhile it is lost to soil
Agricultural chemicals in earth, will also result in soil hardening.The water body treating of pollution by pesticides is more difficult, and up to now, the overwhelming majority uses
Physics, chemical method are acted upon, but these method processing costs are higher, and technical requirements are more strict, difficult in practical application
To promote.
Bioremediation is the effect using microorganism, is decomposed amino benzenes compounds.Due to biological treatment side
Method is more much lower than physics, chemical method cost, and non-secondary pollution, and microorganism has stronger variability and adaptation again
Property, therefore it has turned into the Perfected process of processing polluted-water.The microbial inoculum research of processing metamitron pollution by pesticides water body at present
Less, degradation bacterial agent is actually rare.
The content of the invention
The defects of in order to overcome prior art, answering for metamitron polluted-water is repaired it is an object of the invention to provide a kind of
Close microbial inoculum.
The present invention adopts the following technical scheme that realization：
A kind of composite bacteria agent for repairing metamitron polluted-water, it is prepared in accordance with the following steps：
1）By Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black
Pseudomonad seed liquor is uniform, is then inoculated in inorganic salts domestication culture medium, culture obtains composite bacteria liquid；
2）Composite bacteria liquid is put into the carrier of twice of mass composite bacteria agent is made.
Specifically,
The microbial inoculum is prepared in accordance with the following steps：
1）By Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black
Pseudomonad seed liquor is according to 1-2:2-3:2-3:3-5 volume ratio is uniform, is then inoculated in inorganic salts according to 10% inoculum concentration
Tame in culture medium, shaken cultivation 12h, obtains composite bacteria liquid under the conditions of 30 DEG C, 150rpm；
2）Composite bacteria liquid is put into the carrier of twice of mass, 30min is stirred at 20 DEG C, 4 DEG C stand 24 hours, in bio-carrier
On gradually form flora biomembrane, be filtered dry, produce.
Further,
The preparation method of the carrier comprises the following steps：Polyphenylene oxide is soaked 2 hours with saturated aqueous common salt, is then discharged out liquid
Body, then 6h is soaked with 3wt% sodium hydroxide solution, liquid is discharged, then then adds shell to neutrality with 75% alcohol flushing
Glycan, glycerine and water, it is sufficiently mixed uniformly, is placed in 60 DEG C of water-baths and heats 1h, after vacuum filtration, it is 2% to add concentration
Glutaraldehyde solution, be crosslinked 1h, with water rinse remove unnecessary glutaraldehyde, add concentration be 0.5M CaCl2Aqueous solution room
Temperature stands 12 hours, vacuum drying, obtains carrier.
Preferably, the polyphenylene oxide, chitosan, glycerine, water, glutaraldehyde solution and CaCl2The ratio of the aqueous solution is：1-
2kg:50-80g:5-7kg:2-3kg:5-7L:3-5L。
Preferably, the preparation method of the inorganic salts domestication culture medium comprises the following steps：
Take (NH4)SO4 2g, KH2PO4 1g, Na2HPO4 0.5g, MgSO4 0.2g, trace element solution 1mL, metamitron
200mg, add water to be settled to 1000mL, adjust pH=7.0,121 DEG C of 20min that sterilize, it is standby；
Wherein, trace element solution is formulated as：FeCl3 0.5g, FeSO40.5g, MnSO40.2g, ZnSO40.1g,
CoCl20.1g, adds water to be settled to 1000mL, and filtration sterilization produces.
Preferably, the Dai Erfute acidovorax facilis are ATCC 15668, and the pseudomonas aeruginosa is ATCC 9027,
The atrophy bacillus is ATCC 9372, and the pseudomonas nigrifaciens is ATCC 19375.
Preferably, the Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed
The concentration of liquid and pseudomonas nigrifaciens seed liquor is（1-5）×107cfu/ml。
Beneficial effect of the present invention mainly includes the following aspects：
By in the composite bacteria agent input water body of the present invention, bacterial strain forms biomembrane on bio-carrier surface, carries out metabolism, inhales
Metamitron in attached, decomposition sewage；
The present invention uses four kinds of bacterial strains, and compatibility is reasonable, and synergisticing performance is good, and can mutually promote promotion propagation and enzyme activity, repair
Multiple effect is good；
After composite bacteria agent is handled, metamitron content substantially reduces, and low concentration group clearance can reach 99.8%, high concentration
Group be 99.4%, effect is significantly better than control group, by contrast experiment understand, between each bacterial strain can symbiosis propagation, synergisticing performance
It is good.
The present invention is modified processing by polyphenylene ether, improves porosity and specific surface area, advantages of good adsorption effect, bacterial strain
Adhesive force is high, and the clearance that the clearance of metamitron can reach 99.7%, COD can reach 94.8%, compared with conventional carrier, energy
Enough significantly improve metamitron and COD clearance.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, have below in conjunction with the application
Body embodiment, the technical scheme of the application is clearly and completely described, it is clear that described embodiment is only this Shen
Please part of the embodiment, rather than whole embodiment.Based on the embodiment in the application, those of ordinary skill in the art are not having
There is the every other embodiment made and obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Experimental method in following embodiments, it is conventional method unless otherwise specified.Examination used in following embodiments
Material is tested, is to be commercially available from routine biochemistry reagent channel unless otherwise specified.Quantitative test in following examples,
It is respectively provided with and repeats to test three times, results averaged.
Embodiment 1
A kind of composite bacteria agent for repairing metamitron polluted-water, it is prepared in accordance with the following steps：
Polyphenylene oxide is soaked 2 hours with saturated aqueous common salt, is then discharged out liquid, then 6h is soaked with 3wt% sodium hydroxide solution,
Liquid is discharged, then chitosan, glycerine and water is then added to neutrality with 75% alcohol flushing, is sufficiently mixed uniformly, puts
Heat 1h in 60 DEG C of water-baths, after vacuum filtration, add the glutaraldehyde solution that concentration is 2%, be crosslinked 1h, rinsed and removed with water
Unnecessary glutaraldehyde, add the CaCl that concentration is 0.5M2The aqueous solution is stored at room temperature 12 hours, vacuum drying, obtains carrier；Institute
State polyphenylene oxide, chitosan, glycerine, water, glutaraldehyde solution and CaCl2The ratio of the aqueous solution is：1kg:50g:5kg:2kg:5L:
3L；The aperture of the polyphenylene oxide is 100-300 μm, specific surface area 40-90m2/g；
By Dai Erfute acidovorax facilis ATCC 15668, pseudomonas aeruginosa ATCC 9027, atrophy bacillus ATCC 9372
And pseudomonas nigrifaciens ATCC 19375 passes through inclined-plane culture and seed culture respectively, Dai Erfute acidovorax facilis seeds are obtained
Liquid, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and pseudomonas nigrifaciens seed liquor, control four kinds of seeds
Liquid concentration is 1 × 107cfu/ml。
Take (NH4)SO4 2g, KH2PO4 1g, Na2HPO4 0.5g, MgSO4 0.2g, trace element solution 1mL, metamitron
200mg, add water to be settled to 1000mL, adjust pH=7.0,121 DEG C of sterilizing 20min, it is standby that inorganic salts domestication culture medium is made；Its
In, trace element solution is formulated as：FeCl3 0.5g, FeSO40.5g, MnSO40.2g, ZnSO40.1g, CoCl20.1g, add
Water is settled to 1000mL, filtration sterilization, produces；
Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black is false
Monad seed liquor is according to 1:2:2:3 volume ratio is uniform, is then inoculated in inorganic salts domestication culture medium according to 10% inoculum concentration
In, shaken cultivation 12h, obtains composite bacteria liquid under the conditions of 30 DEG C, 150rpm；
Composite bacteria liquid is put into the carrier of twice of mass, 30min is stirred at 20 DEG C, 4 DEG C stand 24 hours, on bio-carrier
Flora biomembrane is gradually formed, is filtered dry stand-by.
Embodiment 2
A kind of composite bacteria agent for repairing metamitron polluted-water, it is prepared in accordance with the following steps：
Polyphenylene oxide is soaked 2 hours with saturated aqueous common salt, is then discharged out liquid, then 6h is soaked with 3wt% sodium hydroxide solution,
Liquid is discharged, then chitosan, glycerine and water is then added to neutrality with 75% alcohol flushing, is sufficiently mixed uniformly, puts
Heat 1h in 60 DEG C of water-baths, after vacuum filtration, add the glutaraldehyde solution that concentration is 2%, be crosslinked 1h, rinsed and removed with water
Unnecessary glutaraldehyde, add the CaCl that concentration is 0.5M2The aqueous solution is stored at room temperature 12 hours, vacuum drying, obtains carrier；Institute
State polyphenylene oxide, chitosan, glycerine, water, glutaraldehyde solution and CaCl2The ratio of the aqueous solution is：1-2kg:50-80g:5-7kg:
2-3kg:5-7L:3-5L；The aperture of the polyphenylene oxide is 100-300 μm, specific surface area 40-90m2/g；
By Dai Erfute acidovorax facilis ATCC 15668, pseudomonas aeruginosa ATCC 9027, atrophy bacillus ATCC 9372
And pseudomonas nigrifaciens ATCC 19375 passes through inclined-plane culture and seed culture respectively, Dai Erfute acidovorax facilis seeds are obtained
Liquid, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and pseudomonas nigrifaciens seed liquor, control four kinds of seeds
Liquid concentration is 3 × 107cfu/ml。
Take (NH4)SO4 2g, KH2PO4 1g, Na2HPO4 0.5g, MgSO4 0.2g, trace element solution 1mL, metamitron
200mg, add water to be settled to 1000mL, adjust pH=7.0,121 DEG C of 20min that sterilize, it is standby；Wherein, the preparation of trace element solution
For：FeCl3 0.5g, FeSO40.5g, MnSO40.2g, ZnSO40.1g, CoCl20.1g, add water to be settled to 1000mL, cross and filter out
Bacterium, produce；
Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black is false
Monad seed liquor is according to 2:3:3:5 volume ratio is uniform, is then inoculated in inorganic salts domestication culture medium according to 10% inoculum concentration
In, shaken cultivation 12h, obtains composite bacteria liquid under the conditions of 30 DEG C, 150rpm；
Composite bacteria liquid is put into the carrier of twice of mass, 30min is stirred at 20 DEG C, 4 DEG C stand 24 hours, on bio-carrier
Flora biomembrane is gradually formed, is filtered dry stand-by.
Embodiment 3
Experiment one：
Water body example selects：Agricultural planting area of the metamitron polluted-water from In Hangzhou Region of Zhe Jiang Province, set metamitron in water body
Concentration be 100mg/L and 1000mg/L；
Group：The composite bacteria agent of embodiment 1 is experimental group；Control group 1：Dai Erfute acidovorax facilis are not added, and remaining is the same as embodiment 1；
Control group 2：Pseudomonas aeruginosa is not added, remaining is the same as embodiment 1；Control group 3：Atrophy bacillus is not added, and remaining is same
Embodiment 1；Control group 4：Pseudomonas nigrifaciens is not added, remaining is the same as embodiment 1；
Application method：Fence intercept remove solid impurity, adjust the pH of water body between 6.5-7.2, then according to
Every cubic metre of 3g amount adds composite bacteria agent, and processing time is 72 hours.The benzene piperazine for the water discharged after each group is handled
Humulone pollutant load is shown in Table 1：
Table 1
| Index | 100mg/L | 1000mg/L |
| Experimental group | 0.2 | 3.9 |
| Control group 1 | 7.7 | 43.4 |
| Control group 2 | 5.6 | 31.7 |
| Control group 3 | 6.8 | 27.9 |
| Control group 4 | 4.3 | 31.4 |
Conclusion：After composite bacteria agent is handled, metamitron content substantially reduces, and low concentration group clearance can reach 99.8%, height
Concentration group be 99.4%, effect is significantly better than control group 1-4, by contrast experiment understand, between each bacterial strain can symbiosis propagation,
Synergisticing performance is good.
Experiment two：
Water body example selects：Agricultural planting area of the metamitron polluted-water from In Hangzhou Region of Zhe Jiang Province, set metamitron in water body
Concentration be 100mg/L, COD 560mg/L；
Group：The composite bacteria agent of embodiment 2 is experimental group；Control group 1：Carrier is from conventional diatomite support, and remaining is the same as real
Apply example 2；Control group 2：Carrier is from conventional turfy soil：Sawdust is 1:1 complex carrier, remaining is the same as embodiment 2；
Application method：Fence intercept remove solid impurity, adjust the pH of water body between 6.5-7.2, then according to
Every cubic metre of 3g amount adds composite bacteria agent, and processing time is 72 hours.The benzene piperazine for the water discharged after each group is handled
Humulone pollutant load is shown in Table 2：
Table 2
| Index | Metamitron mg/L | COD mg/L |
| Experimental group | 0.3 | 29.3 |
| Control group 1 | 7.1 | 74.8 |
| Control group 2 | 6.9 | 83.6 |
As shown in table 2, the present invention is modified processing by polyphenylene ether, improves porosity and specific surface area, adsorption effect
Good, bacterial strain adhesive force is high, and the clearance that the clearance of metamitron can reach 99.7%, COD can reach 94.8%, with conventional carrier
Compare, metamitron and COD clearance can be significantly improved.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, it is possible to understand that
These embodiments can be carried out with a variety of changes, modification without departing from the principles and spirit of the present invention, replace and become
Type, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of composite bacteria agent for repairing metamitron polluted-water, it is prepared in accordance with the following steps：
1）By Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black
Pseudomonad seed liquor is uniform, is then inoculated in inorganic salts domestication culture medium, culture obtains composite bacteria liquid；
2）Composite bacteria liquid is put into the carrier of twice of mass composite bacteria agent is made.
2. composite bacteria agent according to claim 1, it is characterised in that the composite bacteria agent prepare in accordance with the following steps and
：
1）By Dai Erfute acidovorax facilis seed liquor, pseudomonas aeruginosa seed liquor, atrophy bacillus seed liquor and black
Pseudomonad seed liquor is according to 1-2:2-3:2-3:3-5 volume ratio is uniform, is then inoculated in inorganic salts according to 10% inoculum concentration
Tame in culture medium, shaken cultivation 12h, obtains composite bacteria liquid under the conditions of 30 DEG C, 150rpm；
2）Composite bacteria liquid is put into the carrier of twice of mass, 20 DEG C stir 30min, 4 DEG C stand 24 hours, on carrier by
Flora biomembrane is gradually formed, is filtered dry, produces.
3. composite bacteria agent according to claim 2, it is characterised in that the preparation method of the carrier comprises the following steps：
Polyphenylene oxide is soaked 2 hours with saturated aqueous common salt, is then discharged out liquid, then 6h, discharge are soaked with 3wt% sodium hydroxide solution
Liquid, then chitosan, glycerine and water are then added, is sufficiently mixed uniformly, is placed in 60 to neutrality with 75% alcohol flushing
Heat 1h in DEG C water-bath, after vacuum filtration, add the glutaraldehyde solution that concentration is 2%, be crosslinked 1h, rinsed with water remove it is unnecessary
Glutaraldehyde, add concentration be 0.5M CaCl2The aqueous solution is stored at room temperature 12 hours, vacuum drying, obtains carrier.
4. composite bacteria agent according to claim 3, it is characterised in that the polyphenylene oxide, chitosan, glycerine, water, glutaraldehyde
Solution and CaCl2The ratio of the aqueous solution is：1-2kg:50-80g:5-7kg:2-3kg:5-7L:3-5L.
5. composite bacteria agent according to claim 2, it is characterised in that the preparation method bag of the inorganic salts domestication culture medium
Include following steps：
Take (NH4)SO4 2g, KH2PO4 1g, Na2HPO4 0.5g, MgSO4 0.2g, trace element solution 1mL, metamitron
200mg, add water to be settled to 1000mL, adjust pH=7.0,121 DEG C of 20min that sterilize, it is standby；
The compound method of the trace element solution is：FeCl3 0.5g, FeSO40.5g, MnSO40.2g, ZnSO40.1g,
CoCl20.1g, add water to be settled to 1000mL, filtration sterilization, produce.
6. composite bacteria agent according to claim 2, it is characterised in that the Dai Erfute acidovorax facilis are ATCC 15668,
The pseudomonas aeruginosa is ATCC 9027, and the atrophy bacillus is ATCC 9372, and the pseudomonas nigrifaciens is
ATCC 19375。
7. composite bacteria agent according to claim 2, it is characterised in that the Dai Erfute acidovorax facilis seed liquor, verdigris are false
The concentration of unicellular bacterium seed liquor, atrophy bacillus seed liquor and pseudomonas nigrifaciens seed liquor is（1-5）×
107cfu/ml。
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| CN115094014A (en) * | 2022-08-25 | 2022-09-23 | 江苏朴厚环境工程有限公司 | Ochrobactrum and microbial inoculum thereof and application of Ochrobactrum in pesticide wastewater treatment |
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| CN105920773A (en) * | 2016-04-26 | 2016-09-07 | 安徽农业大学 | Bactericide used for degrading triazine herbicides and preparation method thereof |
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| CN105920773A (en) * | 2016-04-26 | 2016-09-07 | 安徽农业大学 | Bactericide used for degrading triazine herbicides and preparation method thereof |
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| 韩丽珍: ""除草剂2，"4-滴微生物降解研究进展"", 《农药》 * |
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| CN115094014A (en) * | 2022-08-25 | 2022-09-23 | 江苏朴厚环境工程有限公司 | Ochrobactrum and microbial inoculum thereof and application of Ochrobactrum in pesticide wastewater treatment |
| CN115094014B (en) * | 2022-08-25 | 2022-11-25 | 江苏朴厚环境工程有限公司 | Ochrobactrum pallidum, microbial inoculum thereof and application of Ochrobactrum pallidum in pesticide wastewater treatment |
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