CN107589268A - CDV antibody Quantitative detection card and application method - Google Patents
CDV antibody Quantitative detection card and application method Download PDFInfo
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- CN107589268A CN107589268A CN201711054386.4A CN201711054386A CN107589268A CN 107589268 A CN107589268 A CN 107589268A CN 201711054386 A CN201711054386 A CN 201711054386A CN 107589268 A CN107589268 A CN 107589268A
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Abstract
The invention discloses a kind of CDV antibody Quantitative detection card and application method, including detection card shell and the test strips being assemblied in detection card shell, the test strips include the plastic bottom board with pressure sensitive adhesive, paste sample pad successively on bottom plate, labeling pad, nitrocellulose filter and blotting paper, the labeling pad is made up of carrier substrate and label, label is to spray lanthanide fluoro in carrier substrate to detect the tunic that microballoon and lanthanide fluoro Quality Control microballoon are formed, it is detection line that CDV H protein antigen is coated with the nitrocellulose filter, it is nature controlling line to be coated with rabbit-anti chicken lgY antibody, label is the fluorescence Quality Control microballoon of the fluoroscopic examination microballoon and mark chicken lgY antibody that are marked with CDV structural proteins H protein recombinant antigen.The achievable CDV antibody scene Quantitative detection of the present invention, has higher practical value and promotional value.
Description
Technical field
The present invention relates to one kind detection card and use, more particularly to a kind of CDV antibody Quantitative detection card
And application method.
Background technology
Canine distemper（Canine Distemper, CD）By CDV（Canine Distemper virus, CDV）
Infection causes.CDV is under the jurisdiction of Paramyxoviridae Morbillivirus, is that one kind is single-stranded, linearly, the negative adopted RNA virus with cyst membrane,
It is in mostly pleomorphic type, size is between 100-250 nm.CDV is made up of 6 structural proteins, is respectively：N protein（Nucleocapsid
Albumen）, P albumen（Phosphoprotein）, M albumen（Stromatin）, F protein（Fusion protein）, H protein（Hemagglutinin）With L albumen
（Large protein）, wherein N, P and L albumen and viral RNA form nucleocapsid；F protein is one of the glycoprotein on CDV surfaces, is main
Heterotypic immunity cross-reacting antigen, mediate between its cyst membrane and cell membrane it is mutual merge, mediate viral infection；H protein belongs to II
Class glycoprotein, its amino acids of N-terminal 35~55 form the exclusive hydrophobic anchor region of the albumen, have double action, that is, remove
Have outside the function of anchorage zone, and the main signal peptide of transdermal delivery.H albumen produces the mistake of neutralizing antibody in induction body
Played an important role in journey, while be the important antigen of anti-CDV infection immunities again.
In recent years, being significantly increased with China army dog, police dog, experimental dog and pet dog breeding amount, and strange land are handed over
Stream increases, and the incidence of disease and fatal rate of the canine distemper in China dog have elevated trend, and clinical manifestation also with the past
It is different.Sick dog is characterized by being presented two-phase heat type, rhinitis, serious digestive disorder and respiratory inflammation etc..Pup
The death rate more than 90%, into dog the death rate more than 50%.Encephalitis can occur for a small number of cases.In addition to canid, Mustelidae, wash
The many animals such as Ursidae and giant panda section also can infection morbidity.Therefore, CDV is that currently raising pets industry, economic animal are cultivated
The maximum epidemic disease of industry harm is viewed and admired in industry and zoo.
The prevention and control of CDV are mainly vaccine immunity, and vaccine immunity animal is with that will produce knot in infection animal body
Structure protein antibodies, therefore the diagnostic method for detecting structural proteins neutralizing antibody can determine that resistance of the animal to Strain.Simultaneously
It is that the daily monitoring to colony selects vaccine, assessment immune programme for children reasonability, grasp animal to be good for that daily monitoring vaccine, which produces antibody,
The important means of health state, at the same also can from side reflect raising pet it is whether reasonable, and vaccinate in good time it is main according to
According to.
In the market have using detection CDV neutralizing antibody Kit, but ELISA kit need ELIASA,
Incubation reaction condition, board-washing condition, operation element environment and professional and technical personnel, the preceding place that sample also needs to complexity is detected in addition
Reason, such as gather blood, separation serum etc.；And quickly collaurum is although easy to detect, professional and technical personnel and working environment are not required to,
But its sensitivity detected is low, and can only be qualitative, and detection range is narrow.And the purpose of patent of the present invention is exactly to overcome the class of the above two to try
The respective shortcoming of agent box, by shirtsleeve operation, scene is quick, accurate, quantitative with sensitivity（Sxemiquantitative）Analysis, is realized in dog
The Site Detection of raising.
The content of the invention
It is an object of the invention to provide a kind of CDV antibody Quantitative detection card and application method, glue is overcome
The respective shortcoming of body gold rapid detection card and Kit, by shirtsleeve operation, realize dog scene is quick, accurate, Gao Ling
The quick quantitative detection in ground, so as to be fully solved in place of above-mentioned the deficiencies in the prior art.
The purpose of the present invention is realized by following technical proposals：
A kind of CDV antibody Quantitative detection card, including the test paper for detecting card shell and being assemblied in detection card shell
Bar, the test strips include the plastic bottom board with pressure sensitive adhesive, paste sample pad, labeling pad, cellulose nitrate successively on bottom plate
Plain film and blotting paper, the labeling pad are made up of carrier substrate and label, and label is that spraying group of the lanthanides is glimmering in carrier substrate
Light detects the tunic that microballoon and lanthanide fluoro Quality Control microballoon are formed, it is characterised in that：Dog is coated with the nitrocellulose filter
Distemper virus recombinant antigen is detection line, and coating rabbit-anti chicken lgY antibody is nature controlling line, and label is to be marked with CDV knot
The fluoroscopic examination microballoon of the recombinant antigen of structure albumen H protein and the fluorescence Quality Control microballoon of mark chicken lgY antibody.
Further, the CDV recombinant antigen is CDV structural proteins H protein, and it is expressed for multi-epitope
Recombinant protein.
Further, the rabbit-anti chicken lgY antibody that nature controlling line uses on the nitrocellulose filter for rabbit-anti chicken lgY lgG.One
The method that kind prepares above-mentioned CDV structural proteins, using bioinformatics technique（Genbank）To CDV not
H protein gene with lineage strain is compared, and confirms CDV H protein gene order, a knot primer is designed, through PCR
Purpose H gene is amplified, H gene is subcloned on Ppic9K carriers, converts TOP10 competence, positive gram of PCR, digestion identification
Grand, Sal I linearizes recombinant shuttle plasmid pPIC9k-H, and then electricity turns GS115 competence, and multicopy is carried out using G418 resistances
Integron preliminary screening, extract genome and do PCR identifications, obtain positive strain, after induction fermentation, supernatant zymotic fluid carries
Pure target H protein, Prepare restructuring molecular weight of albumen is determined through SDS-PAGE electrophoresis detections, ELISA detections determine that protein immunization is lived
Property.
A kind of method for preparing above-mentioned CDV structural proteins, using bioinformatics technique principle, computation
The structural proteins H antigen genes of CDV are compared machine, and Epitope prediction, screening and design canine distemper
The specific antigenic peptide sequence of virus structural protein H protein, and carry out artificial chemical synthesis Antigenic Peptide.
A kind of application method of above-mentioned detection card, the detection card are quantitative chromatography detection card, will contain CDV
The prepare liquid of antibody is instilled in the well of detection card, chromatographs 15 minutes, then detection card is swept using chromatogram scanner
Retouch, chromatogram scanner will scan the data obtained and radio to client, and detection card is calculated according to scan data in client
The fluorescence signal intensity value of upper nature controlling line and detection line, while client obtains detected CDV antibody from cloud platform
Standard curve, client are calculated according to the contrast relationship of standard curve and nature controlling line and the fluorescence signal intensity value of detection line
The content of CDV antibody in prepare liquid.
Preferably, the standard curve is to import cloud platform in the following way, a collection of detection card is prepared first, and
CDV antibody standard substance corresponding to preparation, block chromatography standard items with detection and detected with chromatogram scanner, computed tomography scanning
Instrument will detect obtained data processing and pass to client, and detection line corresponding to standard items and nature controlling line is calculated in client
Fluorescence signal intensity value, and returned according to this data so as to obtain the standard curve of CDV antibody, client will
Standard curve is transmitted to cloud platform and stored.
Preferably, the standard curve that the client is calculated forms a file, and corresponding generation bar code, visitor
Family end can extract the standard curve by scanning bar code from cloud platform.
Explanation of nouns：
Rabbit-anti chicken lgY lgG：Rabbit-anti chicken lgY immunoglobulin G
Chicken lgY antibody：Chicken yolk antibody
Compared with prior art, the beneficial effects of the present invention are：High sensitivity, batch internal difference and difference between batch of detection are small, have compared with
Good repeatability, stable performance, whole blood sample is both can detect, serum and plasma sample can also be detected, with RV（Rabies viruses）、
CPV（Canine parvovirus), CPIV（Canine parainfluenza virus）、CAV（Hepatitis infectiosa canis virus), CCV（Canine coronavirus）Do not handed over Deng antibody
Fork reaction.CDV antibody field quick detection can be achieved, there is higher practical value and promotional value, using multilist
The recombinant protein of position expression can prevent missing inspection, improve detection in susceptibility.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of test strips in Fig. 1；
Fig. 3 is Fig. 2 top view；
Fig. 4 is CDV antibody test standard curve；
Fig. 5 is another structural representation of present invention detection card.
Embodiment
With reference to specific embodiments and the drawings, the present invention is further illustrated.
As shown in Figures 1 to 4, a kind of CDV antibody Quantitative detection card, including be assemblied in shell 10
Test strips, the test strips include the plastic bottom board 1 with pressure sensitive adhesive, paste sample pad successively from left to right on plastic bottom board 1
2nd, labeling pad 3, nitrocellulose filter 4 and blotting paper 5, and the right-hand member of sample pad 2 is ridden on the left end of labeling pad 3, mark
The right-hand member of thing pad 3 is ridden on the left end of nitrocellulose filter 4, and the left end of blotting paper 5 is ridden on the right-hand member of nitrocellulose filter 4.Institute
The recombinant antigen for stating coating CDV structural proteins on nitrocellulose filter 4 is detection line 6, is coated with rabbit-anti chicken lgY antibody
For nature controlling line 7, counter sample pad 2 opens up well 11 on shell 10, corresponds to detection line 6 and matter on nitrocellulose filter 4
Control line 7 opens up form hole 12.
The CDV recombinant antigen is CDV structural proteins H, and it is the recombinant protein of multi-epitope expression.
The rabbit-anti chicken lgY antibody that nature controlling line uses on the nitrocellulose filter 4 for rabbit-anti chicken lgY lgG.
The labeling pad 3 is made up of carrier substrate and label, and label is to be marked with CDV structure H protein
Recombinant antigen fluoroscopic examination microballoon and mark chicken lgY antibody fluorescence Quality Control microballoon.
CDV antibody, the label and cellulose nitrate of CDV structural proteins H protein antigen in blood sample
It is to detect CDV in dog blood sample by dual-antigen sandwich method that CDV structural protein antigen is coated with plain film
Antibody.
As a kind of optimal technical scheme, it is described detection card the surface of shell 10 correspond to blotting paper 5 position offer it is more
Individual through hole 13, be advantageous to solvent and volatilize shell 10, it is ensured that testing result is accurate, and the detection card shell for solving prior art is adopted
The technical problem that can not be volatilized away with enclosed construction, solvent, referring to Fig. 5.
In order to ensure volatilization effect, equidistantly uniformly opened in the region that the surface of shell 10 matches with the size of blotting paper 5
If through hole 13.
As another optimal technical scheme, the detection card body, which tilts, to be fixed in shell 10, and sample pad 2 is in
Low side, blotting paper 5 are in high-end.This structure causes the testing sample reduced velocity flow instilled from well 11, slowly toward upper strata
Analysis, ensures the enough chromatography time, can effectively prevent that liquid sample flow is too fast and influences testing result, avoid the too fast stream of sample
Entering nitrocellulose filter 4 causes testing result to fail.Because if testing sample flow velocity is too fast, easily cause to chromatograph it is insufficient,
And then influence testing result.The detection inclined angle of card body is specifically designed according to the difference of testing sample.
The eukaryotic expression of embodiment one, CDV structural proteins H protein
Step 1: the PCR of design of primers and target gene is expanded
Specific forward primer is designed according to the CDV H gene nucleotide sequence openly delivered in GenBank databases
SP1：5'-TCGAATTCATGAAAAAACTATTTGTG-3';Anti-sense primer SP2：5'-
TTGCGGCCGCTTACATCACATGGCGT-3', it is restriction enzyme site that italic, which blackens part,.Pichia P1：5'-
GACTGGTTCCAATTGACAAGC-3'；P2：5'-GCAAATGGCATTCTGACATCC-3'.With the hemagglutinin base of synthesis
Cause（Attachment protein gene, H）Genomic clone is that template is template, and it is anti-that primer SP1, SP2 enter performing PCR amplification
Should, final product checks PCR primer with 0.8% agarose gel electrophoresis.
Step 2: cloning vector TS structure and identification
Purpose fragment is after gel reclaims kit reclaims, and 16 DEG C are connected with pBS-T carriers, converts E. coli competent
TOP10, through blue hickie bed board screening, PCR amplification, double digestion identification, sequencing analysis judge recombinant plasmid successfully whether build and
The direction of fragment insertion vector.
Step 3: expression vector pPIC9k-H structure and identification
With restriction enzyme EcoR I, the double digestion TS plasmids of Not I and shuttle plasmid pPIC9k, pPIC9k and H pieces is separately recovered
Section, 16 DEG C of connections overnight, are converted E. coli competent TOP10, are screened using kalamycin resistance bed board, take 1ul plasmids to build
The recombinant plasmid that vertical PCR reaction systems, identification obtain.
Step 4: recombinant expression carrier transformed yeast GS115 competence
PPIC9k-H recombinant plasmids are linearized through Sal I, and its electricity is converted into freshly prepared Pichia pastoris GS115 competent cell
In, competent cell concentration is about 5.5 × 106 ~6.5 × 106Individual/μ L, then, coating MD flat boards, 29 DEG C of incubations 2~
6d。
Step 5: PCR identification positive integration
In screening resistance integron on YPD (G418) flat board of various concentrations, G418 concentration gradients are 0,0.25,
0.5mg/mL, until 6.0mg/mL.YPD (G418) liquid is fallen within from some single bacteriums of picking on 6.0mg/mL G418 YPD flat boards
Medium culture is stayed overnight, and genome is extracted with Yeast genome extracts kit, is entered performing PCR with Pichia P1, P2 primers and is expanded
Increase, identification positive integration, while set shuttle plasmid pPIC9k controls.
Step 6: the induced expression of positive integration and the determination of optimum expression time
The single bacterium colony of picking positive integration, is inoculated in 10mLBMGY culture mediums, 29 DEG C, 200 r/min shaken cultivations to OD600
For 3~6.Then low-speed centrifugal (3000r/min), outstanding thalline is rushed to OD with BMMY culture mediums600It is worth for 1,29 DEG C, 200r/min
Shaken cultivation, 100% methanol is added per 24h to final concentration of 1%.In the 24h after induction, it is thin to take out 1mL at interval of 24h
Bacterium culture supernatant, protein concentration in supernatant is determined with Effendorf ultraviolet specrophotometers, according to formula:Protein concentration
(g/L）= (1.55OD280-0.75OD260）×（Extension rate) total protein concentration is calculated, determine that the expressing quantity highest time is
Optimum expression time.
Step 7: the SDS-PAGE detections of H protein
Trichloroacetic acid (TCA) precipitates 1mL supernatants, adds 2 × SDS40 μ L sample-loading buffers in precipitation, boils 10min, 12%SDS-
PAGE electrophoresis detections.
Embodiment two, CDV structural proteins H protein prepare antigen using specific synthetic peptide
A kind of method for preparing above-mentioned CDV structural proteins H protein, comprises the following steps：
Step 1: the sequence analysis to CDV structural proteins H protein：Utilize bioinformatics mhc class i molecular prediction：
Solved by related web site or appliance computer software is to CDV-H sequential analysis of protein, understand its hydrophily, hydrophobicity, structure
The parameters such as the accessibility in domain, the changeability of sequence, α spirals, β-bend, antigenicity, then comprehensive analysis and the method for homologous modeling it is pre-
Its tertiary structure is surveyed, therefrom predicts antigen-reactive epitope and amino acid residue, is integrated with Peptide synthesizer complexity
Analysis design.Epitope of the every polypeptide chain at least containing a prediction, designs 4 antigen epitope polypeptide fragments altogether.
Step 2: utilize Protein Tec. companies（A company of the U.S.）PS3 type Peptide synthesizers, using FMOC
The antigen epitope polypeptide fragment that solid phase synthesis of peptide reaction principle synthesis step one designs, then cuts polypeptide from FMOC resins
Get off, a plurality of polypeptide fragment connected again by condensation reaction, finally side chain protecting group be cleaved with lysate,
Purifying just obtains synthesized polypeptide, and the H protein for determining CDV structural proteins is tested with Western blot.
Embodiment three, the detection card for preparing CDV antibody
Prepare CDV antibody test standard curve：Configure the calibration solution containing CDV antibody（Pyreticosis containing hundstaupe
Malicious antibody standard substance）6 parts, concentration is respectively 0,1/1024,1/256,1/64,1/16,1/4（CDV antibody standard substance
Doubling dilution）.The calibration solution of above-mentioned various concentrations is separately added into the well of the detection card assembled, chromatographed 15 minutes
Afterwards, detected by chromatogram scanner, the testing result that 6 times are obtained calculates standard items by client process, client
The fluorescence signal intensity value of corresponding detection line and nature controlling line, and CDV is made according to the progress linear regression of this data and resisted
The standard curve of body, standard curve that client is calculated form a file, and it is corresponding generate bar code, client will be with
Bar code is the file of filename（Include standard curve）Transmit to cloud platform and store, referring to Fig. 4.
The standard curve that the client is calculated forms a file, and corresponding generation bar code, client pass through
Scanning bar code can extract the standard curve from cloud platform.
The application method of the detection card：By measuring samples（By taking serum as an example）1 is pressed with sample diluting liquid:50 dilution proportions,
The sample 80ul diluted is added in well 11, in the presence of blotting paper 5, sample is from sample pad 2 to the direction of blotting paper 5
It is mobile.15min is chromatographed in the case where avoiding strong illumination, then detection card is detected with chromatogram scanner, chromatography is swept
The fluorescence signal that instrument obtains detection line 6 and nature controlling line 7 is retouched, detection data are passed to client by chromatogram scanner by bluetooth, visitor
Family end calculates the fluorescence signal intensity value of detection line and the fluorescence signal intensity value of nature controlling line according to detection data, and client is led to
The bar code for over-scanning this batch of detection card obtains the standard curve of detected CDV antibody from cloud platform, client according to
CDV in prepare liquid is calculated in the contrast relationship of standard curve and nature controlling line and the fluorescence signal intensity value of detection line
The content of antibody, referring to Fig. 4 standard curve.Client can be mobile phone or tablet personal computer.
The preparation method of above-mentioned detection card is as follows：
Step 1, nitrocellulose filter is pasted onto on PVC bottom plates, using a film metal spraying special purpose machinery on nitrocellulose filter
The lgG that spraying has been diluted to 0.5mg/ml goat-anti chicken lgY forms nature controlling line and has been diluted to 0.5mg/ml CDV
Structural proteins H recombinant protein forms detection line, and discharge rate is 1 μ l/cm, is then baked 8 hours at a temperature of 37 DEG C；
Step 2, preparation are marked with chicken lgY lanthanide fluoro microballoon and are marked with the restructuring of CDV structural proteins H protein
The lanthanide fluoro microballoon of antigen, 1mL lanthanide fluoro microballoon is added to 50mg MES（2-（N- morpholines）Ethyl sulfonic acid）Buffering
Liquid（0.1M, pH7.0）In, add 10mg carbodiimides（EDC）Stirred with 10mg n-hydroxysuccinimides sulfonate sodium molten
Solution, room temperature reaction carries out centrifugally operated after 30 minutes, by centrifugal sediment 50mM borate buffers（pH8.2）Redissolve, add
The chicken lgY that 2mg dialysed, stirring reaction 24 hours at ambient temperature, after being then centrifuged for, closing, then preserved in dilution
（Conservation environment temperature is 2~8 DEG C）, produce the lanthanide fluoro microballoon for being marked with chicken lgY；Dog is marked using above-mentioned same method
The lanthanide fluoro microballoon of the recombinant antigen of distemper virus structural proteins H protein；
Step 3, be marked with chicken lgY and be marked with CDV H protein recombinant antigen lanthanide fluoro microballoon and difference
The μ g/ml of concentration 0.1 and 3 μ g/ml are diluted to, is sprayed on using a film metal spraying machine in carrier substrate and forms label pad, is sprayed
Measure as 2.5 μ l/cm, then baked 8 hours at a temperature of 37 DEG C；
Step 4, sample pad, label pad, blotting paper are bonded on PVC bottom plates successively, are assembled into kilocalorie, then cut with cutter
The test strips wide into 5mm, it is assemblied in detection card shell, that is, obtains this CDV antibody test card.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (8)
1. a kind of CDV antibody Quantitative detection card, including the examination for detecting card shell and being assemblied in detection card shell
Paper slip, the test strips include the plastic bottom board with pressure sensitive adhesive, and it is fine to paste sample pad, labeling pad, nitric acid successively on bottom plate
Plain film and blotting paper are tieed up, the labeling pad is made up of carrier substrate and label, and label is to spray group of the lanthanides in carrier substrate
The tunic that fluoroscopic examination microballoon and lanthanide fluoro Quality Control microballoon are formed, it is characterised in that：It is coated with the nitrocellulose filter
CDV H protein antigen is detection line, and coating rabbit-anti chicken lgY antibody is nature controlling line, and label is to be marked with hundstaupe pyreticosis
The fluoroscopic examination microballoon of the recombinant antigen of malicious H protein and the fluorescence Quality Control microballoon of mark chicken lgY antibody.
2. CDV antibody Quantitative detection card according to claim 1, it is characterised in that：The hundstaupe pyreticosis
Malicious structural proteins are CDV structural proteins H proteins, and it is the recombinant protein of multi-epitope expression.
3. CDV antibody Quantitative detection card according to claim 1, it is characterised in that：The cellulose nitrate
The rabbit-anti chicken lgY antibody that nature controlling line uses on plain film for rabbit-anti chicken lgY lgG.
A kind of 4. method for preparing the H protein of CDV structural proteins described in claim 2, it is characterised in that：Using life
Thing informatics technology（Bioinformatics）The H protein gene of CDV difference lineage strain is compared, confirmed
CDV H protein gene order, pair of primers is designed, purpose H gene is amplified through PCR, H gene is subcloned on
Ppic9K carriers, convert TOP10 competence, and PCR and digestion identification positive colony, Sal I linearize recombinant shuttle plasmid
PPIC9k-H, then electricity turn GS115 competence, utilize G418 resistances carry out the sub- preliminary screening of multi-copy integration, extract genome
And PCR identifications are done, and positive strain is obtained, after induction fermentation, supernatant zymotic fluid purification target H protein, through SDS-PAGE electrophoresis
Detection determines Prepare restructuring molecular weight of albumen.
A kind of 5. method for preparing the H protein of CDV structural proteins described in claim 2, it is characterised in that：Using life
Thing informatics technical principle, the structural proteins H gene of CDV is compared appliance computer, and epitope
Prediction, screening and the design specific antigenic peptide sequence of CDV H protein, and carry out artificial chemical synthesis Antigenic Peptide.
A kind of 6. application method of the detection card of claim 1 or 2 or 3 or 4, it is characterised in that：The detection card is quantitative
Chromatography detection card, the prepare liquid containing CDV antibody is instilled in the well of detection card, chromatographs 15 minutes, then make
Detection card is scanned with chromatogram scanner, chromatogram scanner radios to client, client by the data obtained is scanned
It is calculated the fluorescence signal intensity value of the upper nature controlling line of detection card and detection line according to scan data, while client is from cloud platform
The standard curve for being detected CDV antibody is obtained, client is believed according to standard curve and nature controlling line and the fluorescence of detection line
The content of CDV antibody in prepare liquid is calculated in the contrast relationship of number intensity level.
7. application method according to claim 6, it is characterised in that：The standard curve is to import cloud in the following way
Platform, a collection of detection card, and CDV antibody standard substance corresponding to preparation are prepared first, block chromatography standard items with detection
And detected with chromatogram scanner, the data processing that detection obtains is passed to client by chromatogram scanner, and client is calculated
The fluorescence signal intensity value of detection line corresponding to standard items and nature controlling line, and returned according to this data so as to obtain canine distemper
Standard curve is transmitted to cloud platform and stored by the standard curve of antiviral antibody, client.
8. application method according to claim 7, it is characterised in that：The standard curve that the client is calculated is formed
One file, and corresponding generation bar code, client can extract the standard curve by scanning bar code from cloud platform.
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| CN108333378A (en) * | 2018-02-09 | 2018-07-27 | 中山市点知生物科技有限公司 | The acquisition methods and system of standard curve |
| CN108845128A (en) * | 2018-05-04 | 2018-11-20 | 广州敏捷生物技术有限公司 | Immunofluorescence for detecting canine distemper virus antigen chromatographs detection card and preparation method |
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| CN113358867A (en) * | 2021-04-26 | 2021-09-07 | 北京润博福得生物科技发展有限公司 | Novel detection composition, detection reagent and detection kit for coronavirus neutralizing antibody |
| CN114047332A (en) * | 2021-10-18 | 2022-02-15 | 中国农业科学院特产研究所 | Canine distemper virus antibody detection kit, gene for coding canine distemper virus nucleoprotein and application of gene |
| CN116008543A (en) * | 2022-10-31 | 2023-04-25 | 北京天之泰生物科技有限公司 | Lateral chromatography system for detecting canine distemper virus antibody and preparation method thereof |
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