CN107585880A - A kind of method that high salt phenol wastewater is handled using gamboge coccus enzyme preparation enhancement microbiological - Google Patents
A kind of method that high salt phenol wastewater is handled using gamboge coccus enzyme preparation enhancement microbiological Download PDFInfo
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Abstract
The invention discloses a kind of method that high salt phenol wastewater is handled using gamboge coccus enzyme preparation enhancement microbiological, this method is first tamed using enzyme preparation to activated sludge, then combines enzyme preparation and high salt phenol wastewater is handled.Enzyme preparation can accelerate activated sludge acclimatization speed, improve activated sludge performance, so as to the efficiency of enhancement microbiological processing waste water, prolonging service lifetime of film, be specially：Activated sludge is improved to 180g/L to the tolerable concentration of salt by 100g/L, the tolerable concentration of phenol is improved to 3500mg/L by 2500mg/L, the flora relative abundance of actinomyces door and Bacteroidetes improves in activated sludge, and phenol goes solution rate to improve 40 60% in MBR, the 10d of fouling membrane cycle stretch-out 8.Therefore, gamboge coccus enzyme preparation can be not only used for the quick domestication of activated sludge, also it can be used for the activity for improving functional flora in biological treatment of waste water system, prolonging service lifetime of film, a kind of safe, cheap, efficient method is provided to promote microbial technique combination MBR to handle the popularization and application of complex industrial waste water.
Description
Technical field
The invention belongs to field of industrial waste water treatment, more particularly to one kind is using at gamboge coccus enzyme preparation enhancement microbiological
The method for managing high salt phenol wastewater.
Background technology
Aldehydes matter belongs to aromatic compounds, and primary discharge species includes phenol, chlorophenol and p-nitrophenol, is chemical industry
Caused a kind of hazardous contaminant for being difficult to degrade in production.Because phenolic compound species is various, difficult degradation and has
" three cause " effect, the pollutant and one of 65 kinds of toxic pollutants of 129 kinds of priority acccess controls have been put into it.In recent years, with oil
The industry such as chemical industry, pharmacy, building materials, agricultural chemicals, papermaking, coal gas, electronics, weaving, dyestuff, plastics and coking develops rapidly, containing phenol
Wastewater discharge greatly increases, and is increasingly becoming one of the primary pollution source of current water body.Meanwhile phenol wastewater composition is very multiple
It is miscellaneous, in addition to containing difficult degradation organic contamination, often containing substantial amounts of salinity (Na+、Cl-、Ca+Deng), when total dissolved solid content exists
More than 3.5% phenol wastewater is high salt phenol wastewater.This industrial wastewater containing high salt, persistent organic pollutants is not only right
Environment is caused seriously to pollute, and human body, aquatile and the harm of crops are increasingly sharpened, the heavy damage ecological balance.
For a long time, the improvement of high salt phenol wastewater is a sewage treatment area problem urgently to be resolved hurrily.
Although the Treatment of Phenol Containing Water based on physics and the principles of chemistry, such as absorption method, extraction, membrane separation process, height
Level oxidizing process etc. has obtained a certain degree of application, but because different Phenol for Waste Water species and content have comparatively wide scope, tool
Body apply when exist poorly efficient, cost is high, secondary pollution problems and be extremely difficult to expected treatment effect.Due to microbial degradation
Treatment process has the characteristics that safe efficient, economic and as high salt phenol wastewater harmless treatment study hotspot.But due to
Contain a large amount of difficult for biological degradation in phenol wastewater and have the compound of bio-toxicity, the growth survival of microorganism, such as benzene can be suppressed
Phenol generates significant inhibitory action, while the height in phenol wastewater to the correlation function bacterium such as denitrogenation dephosphorizing in biological treatment system
Content salt inhibits the microbial activity in biological treatment system, causes traditional biological treatment to be extremely difficult to management goal.
Research finds that the micro organism quantity that traditional separation method can be cultivated only accounts for the 1%-15% of active sludge microorganism total amount, and
Contain rich and varied phenol hydroxylase gene type in the commercial plant of Phenol-Containing Wastewater Treatment, and utilize traditional enrichment to train
Foster method can not obtain the drop phenol bacterium for encoding low affinity costant.Substantial amounts of potential function flora is because in work but non-cultivate
(viable but non-culturable, VBNC) state and be not studied.In addition, most of separate what is obtained by laboratory
Efficient degrading bacterial strain or enrichment culture body, due to being entered VBNC states by environmental pressure stress, so as in actual waste water processing
Biosystem in show relatively low degrading activity.How microorganism in the biological treatment system of high salt phenol wastewater is improved
Activity, it is current biological treatment high salt phenol wastewater urgent problem to be solved to play its optimal efficiency.
The discovery of gamboge coccus Resuscitation-promoting Factor (resuscitation-promoting factor, Rpf) is considered as
It is the most important breakthrough of recovery culture VBNC state bacterium.Gamboge coccus Rpf, it is a kind of secretory protein, autocrine or side can be passed through
Secretion mode is secreted, and its molecular mass is 16-17kDa.Rpf mechanism of action is it is commonly accepted that Rpf is a kind of lysozyme tool
There is peptide glycan to dissolve enzymatic activity, played a significant role during cell wall lysis.It, which can not only recover, promotes the nearly high G+C's of edge
The growth of Gram-positive (G+) bacterium, G+ bacterium and part Gram-negative (G-) bacterium to low G+C also have preferably recovery to promote
Function.Moreover, gamboge coccus Rpf is as a kind of high efficiency bio-augmentation agent, number is cultivated what picomolar concentrations can make bacterium
Amount increases by more than 100 times and can promote that the growth of cell can be cultivated, and being recovered using Rpf, it is latent in biological treatment of waste water system to promote
It is significant in functional flora.But Rpf protein extractions and the relatively high limiting factor of purifying complex, cost, it need to build
Found economically viable biological reinforcing method.Research shows pure Rpf albumen easy in inactivation, meanwhile, separated in gamboge coccus supernatant
Going out at least two has the albumen of Rpf functions (lysozyme activity), and its molecular weight is all higher than Rpf albumen, it is known that, gamboge coccus born of the same parents
Contain at least three kinds of albumen with lysozyme activity in exotocrine.Therefore, can not only be recovered using gamboge coccus enzyme preparation
Promote the activity of salt tolerant degraded phenol flora, improve the efficiency of bioreactor, or culture screening Efficient salt-tolerant degraded phenol
Bacterial strain provides wider array of separation bacterium source.Meanwhile new thinking is provided using bottleneck for break microbiological treatment technology.
The content of the invention
The purpose of the present invention is that bacterial activity present in the biological treatment system for existing high salt phenol wastewater is low, raw
The deficiency that long speed is slow, degradation efficiency is low, there is provided one kind is given up using gamboge coccus enzyme preparation enhancement microbiological processing high salt containing phenol
The method of water.The method has the characteristics of safe, cheap, efficient.
The purpose of the present invention is achieved through the following technical solutions：One kind strengthens micro- life using gamboge coccus enzyme preparation
The method that thing handles high salt phenol wastewater, this method include：
(1) gamboge coccus (Micrococcus luteus) is inoculated in LMM culture medium fermented and cultureds, obtains exocytosis
Thing, further prepare the gamboge coccus enzyme preparation that activity is 1-2U/mL；
(2) it is that 3-4% is added to using phenol as the inorganic of sole carbon source by volume fraction by the enzyme preparation that step (1) obtains
In salt culture medium, activated sludge is tamed；
(3) sludge after step (2) is tamed is seeded in MBR according to the amount that MLSS is 1-2g/L, and with volume fraction
0.5-1.0% adds enzyme preparation, handles phenol wastewater containing high salt.
Further, the step 1 is specially：
(1.1) gamboge coccus is inoculated in LMM culture mediums, 30 DEG C, 160r/min culture 24-36h, obtains fermentation seed
Liquid.Seed culture fluid is taken 30 DEG C, 160r/min culture 48-72h, to be obtained by 2-4% (v/v) inoculum concentration access LMM nutrient solutions
Obtain gamboge coccus zymotic fluid；
The composition of the LMM culture mediums is：4.0g/L NH4Cl, 1.4g/L KH2PO4, 0.005g/L biotins, 0.02g/
L L-Methionines, 0.04g/L vitamin B1s, 1.0g/L inosines, 0.03g/L MgSO4, 8.75g/L Pfansteihl lithium salts, 1.5ml/
L minerals salting liquid (0.375g/L CuSO4·5H2O, 0.785g/L MnCl2·4H2O, 0.18g/L FeSO4·7H2O,
0.029g/L Na2MoO4·2H2O, 0.089g/L ZnSO4·7H2O), pH 7.5；
(1.2) zymotic fluid centrifugation (8000-10000r/min) 10-15min obtained step 1.1 removes thalline, warp
0.22 μm of filter membrane is sterile filtered, and obtains gamboge coccus mucilage secretion；
(1.3) by mucilage secretion to 30KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, collecting pipe inner membrance bottom
Point liquid (liquid 1), then take liquid 1 to 10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, on collecting pipe inner membrance
Partial liquid (liquid 2), solid ammonium sulfate is added into liquid 2 so that the mass fraction of solid ammonium sulfate is under saturation state
80%, ice-water bath stirring 1h, 8000r/min centrifugation 20min collect precipitation；Then into precipitation, addition is identical with liquid 2 again
The phosphate buffer of volume, to 10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, the liquid of collecting pipe inner membrance upper part
Body, as gamboge coccus enzyme preparation.
It is of the invention with existing enhancement microbiological handle high salt phenol wastewater method compared with, its advantage is：
1st, gamboge coccus enzyme preparation used in the present invention, have cost cheap, easy to operate, safe and non-toxic, green etc.
Feature.
2nd, the present invention can realize the efficient domestication of salt tolerant phenol-degrading activated sludge using gamboge coccus enzyme preparation, and it not only may be used
With potential functional flora in recovery activated sludge, functional flora can also be prevented to enter VBNC states, so as to shorten active dirt
Mud tames the time, and plays the optimal salt tolerant drop phenol performance of activated sludge.
3rd, the optimal performance of indigenous microorganism in MBR can be kept in the present invention using gamboge coccus enzyme preparation, is shortened useless
Treatment time of water, reduce fouling membrane, prolonging service lifetime of film, so as to reduce cost for wastewater treatment.
4th, the present invention in by gamboge coccus enzyme preparation added to salt tolerant phenol-degrading activated sludge domestication culture medium in, by with
The control group for being not added with enzyme preparation is contrasted, and shows that gamboge coccus enzyme preparation can significantly improve taming and dociling for salt tolerant phenol-degrading activated sludge
Change efficiency, promote the growth and breeding of salt tolerant drop phenol microorganism.
5th, the present invention in activated sludge acclimatization stage enzyme preparation addition be volume fraction 3-4%, at waste water
In the MBR of reason, its addition is only the 0.5-1% of volume fraction, you can effective to improve activated sludge acclimatization and wastewater treatment
Efficiency, fully demonstrating it has the feature such as rapidity, sustainability, economical, practicality, great application value.
6th, the gamboge coccus enzyme preparation in the present invention can be not only used for quickly taming salt tolerant drop phenol flora, improve microorganism
High salt phenol wastewater efficiency is handled, also can be used for promoting the recovery of functional flora in other industrial wastewaters to grow, to realize micro- life
Thing technology is applied to the biological treatment of organic wastewater with difficult degradation thereby and combined pollution waste water, there is provided it is a kind of rapidly and efficiently, safety and environmental protection,
The cheap method of cost.
In summary, the present invention meets green safe environmental protection concept, realizes enhancement microbiological processing difficult degradation and answers
Pollutant effluents is closed, fully effectively plays the degradation property of indigenous microorganism in biological treatment of waste water system, to promote microorganism skill
The popularization and application of art processing complex industrial waste water provide a kind of safe, cheap, efficient method.
Brief description of the drawings
Fig. 1 is the influence figure for adding gamboge coccus enzyme preparation to activated sludge salt resistant character；
Fig. 2 is to add influence figure of the gamboge coccus enzyme preparation to the resistance to phenol performance of activated sludge.
Embodiment
A kind of method that high salt phenol wastewater is handled using gamboge coccus enzyme preparation enhancement microbiological of the present invention, including it is following
Step：
1st, the preparation of gamboge coccus enzyme preparation
Gamboge coccus activation culture：Gamboge coccus (Micrococcus Luteus IAM14879) used can be purchased from day
This RIKEN Culture Collection.By LMM culture mediums (4.0g/L NH4Cl, 1.4g/L KH2PO4,
0.005g/L biotins, 0.02g/L L-Methionines, 0.04g/L vitamin B1s, 1.0g/L inosines, 0.03g/L MgSO4,
8.75g/L Pfansteihl lithium salts, 1.5ml/L minerals salting liquid (0.375g/L CuSO4·5H2O, 0.785g/L MnCl2·
4H2O, 0.18g/L FeSO4·7H2O, 0.029g/L Na2MoO4·2H2O, 0.089g/L ZnSO4·7H2O), pH 7.5) note
Enter in the ampoule bottle containing gamboge coccus powder, room temperature places 20min, is rule with oese to LMM solid plates.
Gamboge coccus fermented and cultured：The ring of gamboge coccus lawn 1 on picking LMM solid plates is seeded to 20mL LMM liquid
In the 100mL triangular flasks of culture medium, 30 DEG C, 160r/min, 32h is cultivated.Then, inoculation of the seed culture fluid by 3% (v/v) is taken
In amount access LMM nutrient solutions, 30 DEG C, 160r/min culture 52h, gamboge coccus zymotic fluid is obtained.
The preparation of gamboge coccus enzyme preparation：Gamboge coccus zymotic fluid centrifugation (8000r/min, 15min) is removed into thalline, warp
0.22 μm of filter membrane is sterile filtered, the gamboge coccus mucilage secretion of acquisition.Certain volume mucilage secretion is taken to 30KDa
Ultra-filtration centrifuge tube, 7500r/min centrifugation 10min, the liquid (liquid 1) of collecting pipe inner membrance lower part, then take liquid 1 to
10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, the liquid (liquid 2) of collecting pipe inner membrance upper part, by a certain amount of solid
Ammonium sulfate adds liquid 2 so that and the mass fraction of solid ammonium sulfate is 80% under saturation state, and ice-water bath stirs 1h,
8000r/min centrifugations 20min collects precipitation；Then again into precipitation add with the same volume of liquid 2 phosphate buffer, extremely
10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, the liquid of collecting pipe inner membrance upper part, as gamboge coccus enzyme preparation,
It is standby under the conditions of preserving extremely -20 DEG C.
2nd, the detection of gamboge coccus enzyme preparation activity
The drafting of gucosamine standard curve：Take respectively gucosamine standard liquid (the μ g/ml of concentration 50) 0,0.5,1.0,
1.5th, for 2.0mL into colorimetric cylinder, respectively plus distilled water is to 5mL, then adds acetylacetone,2,4-pentanedione solution 1mL solution, puts boiling water bath reaction
25min, after being cooled down rapidly with ice, 3mL absolute ethyl alcohols and 1mL paradime thylaminobenzaldehydes are added, strength vibrates, 20~25 DEG C
1h is placed, not add gucosamine standard liquid pipe as blank control, with the A of the gucosamine standard liquid of other contents527
Draw standard curve.The gucosamine standard curve obtained is Y=0.247X-0.0418 (R2=0.970).
The measure of gamboge coccus enzyme preparation activity：2mg peptide glycans are separately added into 15 μ L gamboge coccus enzyme preparation (experiment
Group) and distilled water (blank group) in, 30 DEG C, after acting on 2h, two groups are moved in 10mL volumetric flasks respectively, adds the 1.5mL concentration to be
6mol/mL HCl, 1h is hydrolyzed in boiling water bath, pH=7.0 is neutralized to sodium hydroxide after cooling, is diluted with water to scale；Then
2mL is taken, adds distilled water 2mL, adds acetylacetone,2,4-pentanedione 1mL, puts after reacting 25min in boiling water bath, is cooled down rapidly with ice；Add
3mL absolute ethyl alcohols and 1mL paradime thylaminobenzaldehydes, strength vibrate, and 20~25 DEG C of placement 1h, determine its test group and sky respectively
White light absorption value of the group at A527 is respectively 0.089 and 0.112, and the content that gucosamine is calculated by standard curve is respectively
26.5 μ g and 31.13 μ g, it is known that the decrement of gucosamine is 4.63 μ g, through formula：Enzyme activity (U/mL)=(sample dilution ×
Glucose osamine content)/(molal weight × sample volume of reaction time × gucosamine)=(20 × 4.63)/(25 ×
221.21×15×10-3)=1.116U/mL, the activity for obtaining gamboge coccus enzyme preparation is 1.116U/mL.
3rd, the domestication and culture of Efficient salt-tolerant phenol-degrading activated sludge
Activated sludge acclimatization：By gamboge coccus enzyme preparation by volume fraction be 3% be added to using phenol as sole carbon source
In minimal medium, the domestication culture medium for the treatment of group is obtained, the composition of wherein minimal medium is：0.5g/L KH2PO4,
0.5g/L K2HPO4, 0.2g/L MgSO4, 1g/L NH4Cl, Trace salts solution 10mL/L (4mg/L MoO3, 28mg/L
ZnSO4·5H2O, 0.02mg/L CuSO4·5H2O, 4mg/L H3BO3, 4mg/L MnSO4·5H2O, 4mg/L CoCl2·
6H2O), phenol and NaCl concentration are depending on requirement of experiment, pH 7.0.Control group is equally used using phenol as sole carbon source
Minimal medium is tamed, and does not add enzyme preparation.
Activated sludge acclimatization process is divided into four-stage：Phenol concentration according to 200mg/L, 500mg/L, 1000mg/L,
1500mg/L gradient is continuously increased, while synchronous raising salinity load, according to 5g/L, 10g/L, 15g/L, 30g/L NaCl
Gradient is incremented by, and the phenol concentration in results of regular determination muddy water supernatant simultaneously observes the change of active pollution index and color.In difference
In the domestication stage, when phenol clearance reaches 100%, activated sludge discharges supernatant after balancing 2d, changes fresh culture medium, carries
Enter next stage domestication after high phenol concentration and salinity.Treatment group and control group tame acquisition respectively can be complete under 30g/L salinity
It is respectively 65d and 76d the time required to the activated sludge of degradable 1500mg/L phenol No. 1 and No. 2.
4th, influence of the gamboge coccus enzyme preparation to activated sludge salt tolerant and resistance to phenol performance
Influence of the enzyme preparation to activated sludge salt resistant character：By above-mentioned two groups of tamed activated sludge connecing according to 2g/L
Kind of amount is seeded to containing identical phenol concentration (1500mg/L) respectively, different salt contents (20,60,100,140,180g/L) synthesis
In waste water, 30 DEG C, 160-180r/min, after cultivating 100h, the phenol residual quantity in waste water is determined respectively, phenol is calculated and removes
Rate.As a result show, sludge is tamed using enzyme preparation, the salt resistant character of activated sludge, activated sludge pair can be significantly improved
The tolerable concentration of salt is improved to 180g/L by 100g/L, sees Fig. 1.
Influence of the enzyme preparation to the resistance to phenol performance of activated sludge：By above-mentioned two groups of tamed activated sludge connecing according to 2g/L
Kind of amount is seeded to containing identical salinity (30g/L) respectively, different phenol concentrations (1500,2000,2500,3000,3500,
In synthetic wastewater 4000mg/L), 30 DEG C, 160-180r/min, after cultivating 100h, the phenol tested respectively in waste water remains
Amount, calculate phenol clearance.As a result show, sludge is tamed using enzyme preparation, the resistance to phenol of activated sludge can be significantly improved
Performance, activated sludge are improved to 3500mg/L to the tolerable concentration of phenol by 2500mg/L, see Fig. 2.
The measure of phenol content：Phenol measure is using 4- amino peace for neighbour method：100 μ L samples are taken, are separately added into 100 μ L
Buffer solution, 200 μ L4- amino peace solution, 200 μ L potassium ferricyanide solutions near, are finally settled to 10mL and stand 15min,
At 510nm light absorption value is determined with ultraviolet specrophotometer.Draw standard curve：Respectively precision measure phenol standard liquid 5.0,7.5,
10.0th, 12.5,15.0,17.5,20.0mL adds 1.0m L cushioning liquid and (weighs 20g ammonium chlorides into 50mL volumetric flasks
NH4Cl is dissolved in 100mL ammoniacal liquor), mix, sequentially add 4-AA solution 2.0m L, potassium ferricyanide solution 2.0m
L, the absorbance of different phenol concentrations is determined under redistilled water constant volume 50mL, 510nm wavelength.The phenol Standard curve obtained is Y
=0.1373X+0.0009 (R2=0.9998).Phenol concentration can be obtained by bringing surveyed absorbance into this standard curve, so as to
Calculate the clearance of phenol.
5th, influence of the gamboge coccus enzyme preparation to acclimated activated sludge Bacterial community
Above-mentioned two groups of acclimated activated sludge samples 1 (addition enzyme preparation) and No. 2 (being not added with enzyme preparation) are subjected to 16S
The high-flux sequence analysis of rRNA genes.The DNA of activated sludge sample uses FastDNATM SPIN Kit for Soil
(Bio101Inc., USA) kit extracts.Using primer 341F5 '-CCTAYGGGRBGCASCAG-3 ' and 806R 5 '-
GGACTACNNGGGTATCTAAT-3 ' amplification 16S rRNA gene V3-V4 hypervariable regions fragment.25 μ L RCR reaction systems are such as
It is 3.2.3.1 described.PCR reaction conditions are：94 DEG C of pre-degenerations 2min, 94 DEG C of denaturation 30s, 57 DEG C of annealing 30s, 72 DEG C of extension 30s,
25 circulations are carried out, 72 DEG C extend 5min, 16 DEG C of holdings eventually.By the parallel PCR primer mixed in equal amounts of each three groups of sample, through quantitative
Double end sequencings are carried out using the platforms of Illumina Miseq 2500 Deng after quality control.Then using Uprase softwares to having
Imitate sequence and carry out activity classification unit (operational taxonomic unit, OTU) cluster, according to 97% similitude by sequence
Row are clustered into OTUs, and the analysis of Bacterial community is carried out from different categorization levels.As a result show, sludge is carried out using enzyme preparation
Domestication, the Bacterial community composition of activated sludge can be changed, as shown in table 1, gamboge coccus enzyme preparation remarkably promotes actinomyces door
(Actinobacteria) and Bacteroidetes (Bacteroidetes) flora growth.
The relative abundance of the different bacterium doors of table 1
| Bacterium door (relative abundance %) | Primary sludge | Treatment group-activated sludge | Control group-activated sludge |
| Proteobacteria | 77.95 | 47.39 | 59.56 |
| Bacteroidetes | 12.27 | 42.01 | 31.06 |
| Actinobacteria | 2.64 | 6.17 | 3.41 |
| Firmicutes | 4.39 | 1.60 | 2.11 |
| Spirochaetes | 0.65 | 0.73 | 0.92 |
| Tenericutes | 0.39 | 0.30 | 0.36 |
| Chrysiogenetes | 0.23 | 0.23 | 0.14 |
| Synergistetes | 0.20 | 0.19 | 0.10 |
| Saccharibacteria | 0.16 | 0.16 | 0.15 |
| Others | 1.12 | 1.22 | 2.19 |
6th, enzyme preparation strengthens the efficiency of MBR processing high salt phenol wastewater
By above-mentioned acclimated activated sludge sample 1 (addition enzyme preparation) and No. 2 (being not added with enzyme preparation) respectively according to MLSS
4 groups of identical membrane bioreactors (MBR) are seeded to for 1g/L amount, treatment group adds the gamboge that volume fraction (v/v) is 0.8%
Coccus enzyme preparation, control group do not add enzyme preparation, obtain 4 sets of processing high salt phenol wastewater devices：
1st set：No. 1 activated sludge+MBR+ adds 0.8% enzyme preparation；
2nd set：No. 1 activated sludge+MBR+ is not added with 0.8% enzyme preparation；
3rd set：No. 2 activated sludge+MBR+ add 0.8% enzyme preparation；
4th set：No. 2 activated sludge+MBR+ are not added with 0.8% enzyme preparation；
Handle NaCl containing 30g/L, 1000mg/L phenol, the waste water that COD 2450mg/L, ammonia nitrogen are 212.5mg/L, control
Temperature processed is 30 DEG C, pH 8, HRT 24h, and water inlet flow velocity is 6mL/min；
1st set of water outlet phenol concentration is 0mg/L, COD 46mg/L, ammonia nitrogen concentration 0.8mg/L；2nd set of water outlet phenol
Concentration is 56mg/L, COD 145mg/L, ammonia nitrogen 7.2mg/L；3rd set of water outlet phenol concentration is 93mg/L, COD 237mg/
L, ammonia nitrogen 10.6mg/L；4th set of water outlet phenol concentration is 124mg/L, COD 302mg/L, ammonia nitrogen 13.7mg/L；And
In the range of maximum transmembrane pressure 20KPa, the 1st set is compared with the 2nd set, and TMP reduces 9KPa, and the 1st set is compared with the 4th set, and TMP is reduced
13KPa, the 1st set of cycle for needing to clean film is 25d, and the 2nd set is 14d, and the 3rd set is 11d, and the 4th set is 7d；As a result enzyme system is shown
Agent addition can substantially slow down fouling membrane speed, prolonging service lifetime of film.It can thus be appreciated that it is anti-to strengthen MBR using gamboge coccus enzyme preparation
Answer the significant effect of device processing high salt phenol wastewater.
In addition, the inoculum concentration in guarantee acclimation sludge is 1-2g/L, the addition of enzyme preparation is 0.5-1.0vol%, you can
Realize efficient waste water first draft disposal ability.
Claims (2)
- A kind of 1. method that high salt phenol wastewater is handled using gamboge coccus enzyme preparation enhancement microbiological, it is characterised in that the party Method includes：(1) gamboge coccus (Micrococcus luteus) is inoculated in LMM culture medium fermented and cultureds, obtains mucilage secretion, Further prepare the gamboge coccus enzyme preparation that activity is 1-2U/mL；(2) it is that the inorganic salts that 3-4% is added to using phenol as sole carbon source are trained by volume fraction by the enzyme preparation that step (1) obtains Support in base, activated sludge is tamed；(3) sludge after step (2) is tamed is seeded in MBR according to the amount that MLSS is 1-2g/L, and with volume fraction 0.5- 1.0% adds enzyme preparation, handles phenol wastewater containing high salt.
- 2. according to the method for claim 1, it is characterised in that the step 1 is specially：(1.1) gamboge coccus is inoculated in LMM culture mediums, 30 DEG C, 160r/min culture 24-36h, obtains fermentation seed liquid.Take Seed culture fluid 30 DEG C, 160r/min culture 48-72h, obtains rattan by 2-4% (v/v) inoculum concentration access LMM nutrient solutions Yellow coccus zymotic fluid；The composition of the LMM culture mediums is：4.0g/L NH4Cl, 1.4g/L KH2PO4, 0.005g/L biotins, 0.02g/L L- Methionine, 0.04g/L vitamin B1s, 1.0g/L inosines, 0.03g/L MgSO4, 8.75g/L Pfansteihl lithium salts, 1.5ml/L ore deposits Matter salting liquid (0.375g/L CuSO4·5H2O, 0.785g/L MnCl2·4H2O, 0.18g/L FeSO4·7H2O, 0.029g/L Na2MoO4·2H2O, 0.089g/L ZnSO4·7H2O), pH 7.5；(1.2) zymotic fluid centrifugation (8000-10000r/min) 10-15min obtained step 1.1 removes thalline, through 0.22 μ M filter membranes are sterile filtered, and obtain gamboge coccus mucilage secretion；(1.3) mucilage secretion to 30KDa ultra-filtration centrifuge tubes, 7500r/min are centrifuged into 10min, collecting pipe inner membrance lower part Liquid (liquid 1), liquid 1 is then taken to 10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, collecting pipe inner membrance upper part Liquid (liquid 2), by solid ammonium sulfate add liquid 2 so that the mass fraction of solid ammonium sulfate be saturation state under 80%, ice-water bath stirring 1h, 8000r/min centrifugation 20min collects precipitation；Then added and the same volume of liquid 2 into precipitation again Long-pending phosphate buffer, to 10KDa ultra-filtration centrifuge tubes, 7500r/min centrifugation 10min, the liquid of collecting pipe inner membrance upper part, As gamboge coccus enzyme preparation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108486238A (en) * | 2018-04-10 | 2018-09-04 | 南京大学 | A kind of biological reinforced functional flora analytic method based on high-flux sequence |
| CN109371692A (en) * | 2018-11-01 | 2019-02-22 | 广州邦葳纺织助剂有限公司 | A kind of processing method of fabric APEO |
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| CN101487003A (en) * | 2009-03-02 | 2009-07-22 | 华南理工大学 | Acid protease |
| WO2016022999A1 (en) * | 2014-08-08 | 2016-02-11 | Aquatech Bioenergy LLC | Systems and methods for wastewater treatment using aquatic plants |
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| CN101487003A (en) * | 2009-03-02 | 2009-07-22 | 华南理工大学 | Acid protease |
| WO2016022999A1 (en) * | 2014-08-08 | 2016-02-11 | Aquatech Bioenergy LLC | Systems and methods for wastewater treatment using aquatic plants |
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| 刘殷东: "添加藤黄球菌SRpf对序批式反应器(SBR)废水处理效果的影响及其机理初探", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486238A (en) * | 2018-04-10 | 2018-09-04 | 南京大学 | A kind of biological reinforced functional flora analytic method based on high-flux sequence |
| CN108486238B (en) * | 2018-04-10 | 2020-10-16 | 南京大学 | High-throughput sequencing-based bioaugmentation functional flora analysis method |
| CN109371692A (en) * | 2018-11-01 | 2019-02-22 | 广州邦葳纺织助剂有限公司 | A kind of processing method of fabric APEO |
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| CN107585880B (en) | 2020-08-07 |
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