CN107582067B - Biological fluorescence developing agent for developing biological material evidence trace on human skin surface and developing method - Google Patents
Biological fluorescence developing agent for developing biological material evidence trace on human skin surface and developing method Download PDFInfo
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- CN107582067B CN107582067B CN201710785697.1A CN201710785697A CN107582067B CN 107582067 B CN107582067 B CN 107582067B CN 201710785697 A CN201710785697 A CN 201710785697A CN 107582067 B CN107582067 B CN 107582067B
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Abstract
The invention relates to a bioluminescence developing agent for developing biological material evidence traces on the surface of human skin and a developing method, wherein the bioluminescence developing agent comprises the following raw materials by mass percent of 100 percent: 0.001-0.005% of indene diketone, 1-3% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 67-72% of petroleum ether. The invention can show the biological material evidence trace on the surface of the skin of the human body by adjusting the formula proportion of the biological fluorescence showing agent; in addition, the invention does not need to be dried at high temperature and control the humidity of the object and the environment, thereby simplifying the display aspect and avoiding the damage to the biological material evidence on the object.
Description
Technical Field
The invention relates to a bioluminescence visualization agent for visualizing a biological evidence trace on the surface of human skin and a visualization method.
Background
In criminal material evidence investigation field, need discover and acquire the criminal material evidence that the suspect left over, these material evidences include fingerprint material evidence, palm print material evidence, can reflect the vestige material evidence of DNA speciality, borrow this and establish the crime fact evidence chain, establish the legal evidence basis for punishing the crime.
In the prior art, the inventor has proposed a method for developing fingerprints by using indandione, see chinese patent CN106802292A, in which a bioluminescence developing agent is used to soak or spray a permeable object, then the permeable object is dried at a temperature of 50-120 ℃ and a relative humidity of less than 40%, the dried permeable object is irradiated by a laser with a wavelength of 532 nm and a broad-spectrum half-height width of less than 1 nm, and the surface of the permeable object is controlled to form an illumination exceeding 30 ten thousand lux, and a biological evidence trace on the permeable object can be developed under a light-blocking filter at 540 nm, wherein the bioluminescence developing agent has a raw material formula, in terms of weight percentage, as follows: 0.02-0.5% of indene diketone, 4-10% of ethyl acetate, 0.5-1.5% of glycerol, 5-15.5% of pure alcohol and 73.5-90% of petroleum ether. The patent can be used for showing biological evidence traces on the surfaces of permeable objects such as napkin paper, toilet paper, thermal sensitive paper, invoice bills, writing paper, cloth, bricks, wood, stones and the like.
However, since a large amount of amino acids exist on the surface of the human body itself, and the biological evidence trace of the patent is developed by laser after the biological fluorescence developing agent reacts with the amino acids remaining on the surface of the evidence, the amino acids of the human body itself cannot be distinguished from the amino acids remaining in the other people by using this principle, and thus the biological fluorescence developing agent of the patent cannot develop the biological evidence trace on the surface of the skin of the human body.
Disclosure of Invention
The invention aims to provide a bioluminescence developing agent capable of developing biological evidence traces on the surface of human skin.
The invention aims to solve another technical problem of providing a method for showing biological evidence traces on the surface of human skin.
The third technical problem to be solved by the invention is to provide an application of the biological fluorescence developing agent in the development of biological evidence traces on the surface of human skin.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention aims to provide a bioluminescence developing agent for developing biological evidence traces on the surface of human skin, which comprises the following raw materials in percentage by mass of 100 percent: 0.001-0.005% of indene diketone, 1-3% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 67-72% of petroleum ether.
Preferably, the formula of the raw materials of the biological fluorescence developing agent is as follows: 0.001-0.005% of indene diketone, 1.5-2.5% of ethyl acetate, 3-4% of glycerol, 22-23% of pure alcohol and 70.5-72% of petroleum ether.
In the invention, the biological material evidence trace comprises a fingerprint, and the fingerprint comprises a fingerprint and a palm print.
In the present invention, pure alcohol refers to absolute ethanol with a mass concentration of more than 99.7%.
The invention also aims to provide a method for showing the biological evidence trace on the surface of the human skin, which comprises the steps of soaking or spraying the surface of the human skin by using the biological fluorescence showing agent, irradiating the surface of the human skin by using laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm, controlling the surface of the human skin to form the illumination of more than 30 ten thousand luxes, and showing the biological evidence trace on the surface of the human skin under the cut-off filter lens at 540 nm.
Preferably, after the surface of the skin of the human body is soaked or sprayed by the biological fluorescence developing agent, the laser is used for irradiating after the surface of the skin of the human body is dried.
Wherein, the drying of the skin surface of the human body refers to the volatilization of the reagent on the skin surface of the human body, and the heating volatilization or the natural volatilization can be adopted.
Further preferably, the human skin surface is dried in an environment of not more than 50 ℃.
Still preferably, said human skin surface is dried in an environment not exceeding 45 ℃.
More preferably, the human skin surface is dried in an environment not exceeding 40 ℃.
Most preferably, the human skin surface is dried at 20-37 ℃.
In the present invention, the human body includes a cadaver and a living body.
Specifically, when the human body is a living body, the soaking or spraying of the bioluminescence developing agent is performed within 1 hour after the biological material evidence is applied to the skin surface of the human body.
The invention has the following emerging principle: the invention utilizes the discovery that the self amino acid content of the human body and the biological fluorescence developing agent can not be displayed under the laser irradiation after reaction through the adjustment of the biological fluorescence developing agent, and the part with high amino acid content can be displayed, thereby realizing the display of the biological evidence trace on the surface of the human body.
For corpses, after the death of people, metabolism stops, the amino acid content on the surface of a human body changes slightly, and at the moment, the amino acid content of the part on which the amino acid of other people is superposed is always higher than that of other parts, so the bioluminescence developing agent can be used for developing biological evidence traces on corpses for a long time after being damaged.
In the case of a living body, since the human body is constantly metabolized, the amino acid content of a portion on which the amino acid of another person is superimposed tends to be the same as the amino acid content of other portions as the self-metabolism proceeds, and therefore, the biological evidence trace needs to be developed within 1 hour for the living body, and the biological evidence trace cannot be developed if the time is too long.
The third purpose of the invention is to provide the application of the biological fluorescence developing agent in the development of biological evidence traces on the surface of human skin.
In the present invention, the human body includes a cadaver and a living body.
Due to the implementation of the technical scheme, compared with the prior art, the invention has the following advantages:
the invention can show biological material evidence trace on the surface of human skin by adjusting the formula proportion of the biological fluorescence developing agent, thereby facilitating the extraction of genetic material, and can show the lines of fingerprints or handprints, thereby facilitating the fingerprint comparison; in addition, the invention does not need to be dried at high temperature and control the humidity of the object and the environment, thereby simplifying the display aspect and avoiding the damage to the biological material evidence on the object.
Drawings
FIG. 1 is a photograph of a fingerprint of example 1;
FIG. 2 is a photograph of a fingerprint of example 2;
FIG. 3 is a photograph of a fingerprint of example 3;
FIG. 4 is a partially enlarged view of a fingerprint photograph of example 3;
FIG. 5 is a photograph of a fingerprint of example 4;
FIG. 6 is a photograph of a fingerprint of comparative example 1;
fig. 7 is a fingerprint photograph of comparative example 2.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the present invention is not limited to these examples.
Example 1
(1) And preparation of the biological fluorescence developing agent: adding 3g of glycerol into 23g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.003g of indandione was sufficiently dissolved in 2g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 71.997g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the bioluminescence developing agent prepared in the step (1) on the skin surface of the dead body, irradiating the dead body by using laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm after the adhering agent volatilizes at room temperature (25 ℃), controlling the illumination of 30 kilogrammes formed on the surface of the dead body, and obtaining a fingerprint shown in figure 1 by a photographic mode under a cut-off filter at 540 nm.
Example 2
(1) And preparation of the biological fluorescence developing agent: adding 4g of glycerol into 23g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.001g of indandione is sufficiently dissolved in 1.5g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 71.499g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) The hands of the other people press the ankle of the living body and then move away; after 10 minutes, spraying the pressed part with the biological fluorescence developing agent prepared in the step (1), then waiting for the adhering agent to volatilize at room temperature (30 ℃), irradiating the part with laser with the wavelength of 532 nm and the spectrum half-height width of less than 1 nm, controlling the surface of the part to form the illumination of 30 kilogrammes, and obtaining the fingerprint shown in figure 2 by a photographic mode under a cut-off filter lens of 540 nm.
Example 3
(1) And preparation of the biological fluorescence developing agent: adding 4g of glycerol into 23g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.005g of indandione is sufficiently dissolved in 2.5g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 70.495g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) The hands of the other people are pressed on the arms of the living body and then are removed; after 30 minutes, spraying the pressed part with the biological fluorescence developing agent prepared in the step (1), then waiting for the attached reagent to volatilize at 40 ℃, irradiating the part with laser with the wavelength of 532 nanometers and the spectrum half-height width of less than 1 nanometer, controlling the surface of the part to form the illumination of 30 kilo-lux, and obtaining a fingerprint by a photographic mode under a cut-off filter lens of 540 nanometers, wherein the enlarged view is shown in figure 3 and figure 4.
Example 4
(1) And preparation of the biological fluorescence developing agent: adding 5g of glycerol into 20g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.001g of indandione is fully dissolved in 3g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 71.999g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) The hands of other people press on the calf of the living body and then are removed; after 50 minutes, spraying the pressed part with the biological fluorescence developing agent prepared in the step (1), then waiting for the attached reagent to volatilize at 40 ℃, irradiating the part with laser with the wavelength of 532 nanometers and the spectrum half-height width of less than 1 nanometer, controlling the surface of the part to form the illumination of 30 kilogrammes, and obtaining the fingerprint shown in figure 5 by a photographic mode under a cut-off filter lens of 540 nanometers.
Comparative example 1
(1) And preparation of the biological fluorescence developing agent: adding 6g of glycerol into 30g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.0008g of indandione is fully dissolved in 0.1g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 63.8992g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) The hands of other people press on the calf of the living body and then are removed; after 5 minutes, spraying the pressed part with the biological fluorescence developing agent prepared in the step (1), then waiting for the adhering agent to volatilize at room temperature (30 ℃), irradiating the part with laser with the wavelength of 532 nm and the spectrum half-height width of less than 1 nm, controlling the surface of the part to form the illumination of 30 kilogrammes, and obtaining the fingerprint shown in figure 6 by a photographic mode under a cut-off filter lens of 540 nm.
Comparative example 2
(1) And preparation of the biological fluorescence developing agent: adding 1.5g of glycerol into 15g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.01g of indandione is fully dissolved in 4g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 79.49g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Pressing the hands of other people on the live calf, and then removing the live calf; after 10 minutes, spraying the pressed part with the biological fluorescence developing agent prepared in the step (1), then waiting for the adhering agent to volatilize at room temperature (30 ℃), irradiating the part with laser with the wavelength of 532 nm and the spectrum half-height width of less than 1 nm, controlling the surface of the part to form the illumination of 30 kilogrammes, and obtaining the fingerprint shown in figure 7 by a photographic mode under a cut-off filter lens of 540 nm.
The present invention has been described in detail in order to enable those skilled in the art to understand the invention and to practice it, and it is not intended to limit the scope of the invention, and all equivalent changes and modifications made according to the spirit of the present invention should be covered by the present invention.
Claims (8)
1. The application of a bioluminescence visualization agent in the visualization of biological evidence traces on the surface of human skin is characterized in that: the biological fluorescence developing agent comprises the following raw materials by mass percent based on 100 percent of the biological fluorescence developing agent: 0.001-0.005% of indene diketone, 1-3% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 67-72% of petroleum ether.
2. Use according to claim 1, characterized in that: the biological fluorescence developing agent is adopted to soak or spray the surface of human skin, then laser with the wavelength of 532 nm and the spectrum half-height width of less than 1 nm is used for irradiating the surface of the human skin, the surface of the human skin is controlled to form the illumination of more than 30 ten thousand luxes, and the biological evidence trace on the surface of the human skin can be developed under the condition of cutting off the filter lens at 540 nm.
3. Use according to claim 2, characterized in that: after the surface of the human skin is soaked or sprayed by the biological fluorescence developing agent, and the surface of the human skin is dried, the laser is used for irradiating.
4. Use according to claim 3, characterized in that: drying the human skin surface in an environment of not more than 50 ℃.
5. Use according to claim 4, characterized in that: drying the human skin surface in an environment of not more than 45 ℃.
6. Use according to claim 5, characterized in that: drying the human skin surface in an environment of not more than 40 ℃.
7. Use according to any one of claims 1 to 6, characterized in that: the human body comprises a cadaver and a living body.
8. Use according to claim 7, characterized in that: when the human body is a living body, the biological fluorescence developing agent is soaked or sprayed within 1 hour after the biological evidence is applied to the surface of the skin of the human body.
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| CN109199399B (en) * | 2018-09-05 | 2021-11-26 | 苏州纳磐新材料科技有限公司 | Fingerprint developing agent and preparation method thereof |
| CN112754477B (en) * | 2019-11-05 | 2021-12-24 | 南京大学 | Latent fingerprint display method based on cesium halide lead perovskite nano material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4236082A (en) * | 1979-01-29 | 1980-11-25 | Palmguard, Inc. | Method and apparatus for recording image details of the palm of a hand |
| JPH01501175A (en) * | 1986-08-27 | 1989-04-20 | ジ・オーストラリアン・ナショナル・ユニバーシティ | Method and apparatus for identifying photoluminescent reflective surfaces in forensic applications |
| US8460742B2 (en) * | 2007-11-29 | 2013-06-11 | University Of Technology, Sydney | Method of developing latent fingerprints |
| CN105105761A (en) * | 2015-09-16 | 2015-12-02 | 福建师范大学 | Chemical appearing method for potential finger prints |
| CN106802292B (en) * | 2017-01-22 | 2019-07-02 | 苏州晓松科技开发有限公司 | A method of showing permeability object biological evidence trace |
| CN106841140B (en) * | 2017-01-22 | 2018-07-24 | 苏州晓松科技开发有限公司 | A kind of biological evidence trace shows extracting method |
| CN106872437A (en) * | 2017-04-14 | 2017-06-20 | 南京工业大学 | MOFs precursor-based fingerprint rapid-visualization reagent and application thereof |
| CN106974659A (en) * | 2017-05-20 | 2017-07-25 | 复旦大学 | A kind of latent fingerprint detection method based on red fluorescence carbon point material |
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