CN107582067A - A kind of bioluminescence visualization reagent and process for show for being used to show human skin's biological evidence vestige - Google Patents
A kind of bioluminescence visualization reagent and process for show for being used to show human skin's biological evidence vestige Download PDFInfo
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- CN107582067A CN107582067A CN201710785697.1A CN201710785697A CN107582067A CN 107582067 A CN107582067 A CN 107582067A CN 201710785697 A CN201710785697 A CN 201710785697A CN 107582067 A CN107582067 A CN 107582067A
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Abstract
It is that the composition of raw materials of described bioluminescence visualization reagent is based on 100% by the quality of described bioluminescence visualization reagent the present invention relates to a kind of bioluminescence visualization reagent and process for show for being used to show human skin's biological evidence vestige:Indandione rodenticide 0.001% ~ 0.005%, ethyl acetate 1% ~ 3%, glycerine 2% ~ 5%, absolute alcohol 20% ~ 25%, petroleum ether 67% ~ 72%.The present invention can show the biological evidence vestige of human skin by the adjustment to bioluminescence visualization reagent formula rate;Also, the present invention, without the humidity of control object and environment, on the one hand simplifies without being dried at high temperature and shows aspect, on the other hand avoid the destruction to biological evidence on object.
Description
Technical field
The present invention relates to a kind of bioluminescence visualization reagent for showing human skin's biological evidence vestige and show
Method.
Background technology
Field is reconnoitred, it is necessary to find and obtain the crime material evidence that suspect leaves in criminal material evidence, and these material evidences include referring to
Line material evidence, palmmprint material evidence, the trace evidence that DNA speciality can be reflected, crime fact chain of evidence is built whereby, is established to punish crimes
Determine legal argument basis.
In the prior art, inventor sees Chinese patent it is proposed that excessively using the method for indandione rodenticide progress fingerprint displaying
CN106802292A, it is soaked or sprayed to permeability object with bioluminescence visualization reagent, then permeability object is existed
Relative humidity be less than 40%, temperature be 50 ~ 120 DEG C in the environment of dry, with wavelength be 532 nanometers, wide spectrum halfwidth degree be less than 1
The laser of nanometer irradiates the dried permeability object, and controls the permeability object surface to form over 30 Wan Leke
The illumination of department, the biological evidence vestige under 540 nanometers of lower section of light optical filterings in described permeability object can show, by weight
Percentage composition meter is measured, the composition of raw materials of the bioluminescence visualization reagent is:Indandione rodenticide 0.02% ~ 0.5%, ethyl acetate 4% ~ 10%,
Glycerine 0.5% ~ 1.5%, absolute alcohol 5% ~ 15.5%, petroleum ether 73.5% ~ 90%.The patent can be used in napkin paper, toilet paper, temperature-sensitive
The permeability object surface biological material evidence vestiges such as paper, invoice document, writing paper, cloth, brick, timber, stone show.
But because human body surface inherently largely has amino acid, and the biological evidence vestige of the patent is to pass through life
Thing fluorescence visualization reagent, therefore, can not using the principle with after the amino acid reaction of material evidence remained on surface, being shown under laser
The amino acid of human body itself and the amino acid that other people leave are made a distinction, therefore, the bioluminescence visualization reagent of the patent can not
Show the biological evidence vestige of human skin.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of human skin biological evidence vestige of can showing
Bioluminescence visualization reagent.
Another technical problem to be solved by this invention is to provide a kind of human skin biological evidence vestige of showing
Process for show.
3rd technical problem to be solved by this invention is to provide a kind of above-mentioned bioluminescence visualization reagent in human body skin
Application in the showing of the biological evidence vestige on surface.
To solve above technical problem, the present invention adopts the following technical scheme that:
It is an object of the present invention to provide a kind of bioluminescence for being used to show human skin's biological evidence vestige to show
Agent, it is that the composition of raw materials of described bioluminescence visualization reagent is based on 100% by the quality of described bioluminescence visualization reagent:Indenes two
Ketone 0.001% ~ 0.005%, ethyl acetate 1% ~ 3%, glycerine 2% ~ 5%, absolute alcohol 20% ~ 25%, petroleum ether 67% ~ 72%.
Preferably, the composition of raw materials of described bioluminescence visualization reagent is:Indandione rodenticide 0.001% ~ 0.005%, ethyl acetate
1.5% ~ 2.5%, glycerine 3% ~ 4%, absolute alcohol 22% ~ 23%, petroleum ether 70.5% ~ 72%.
In the present invention, described biological evidence vestige includes impression of the hand, and impression of the hand includes fingerprint and palmmprint.
In the present invention, absolute alcohol refers to the absolute ethyl alcohol that mass concentration is more than 99.7%.
It is a further object to provide a kind of process for show for showing human skin's biological evidence vestige, adopt
Human skin is soaked or sprayed with described bioluminescence visualization reagent, is then 532 nanometers with wavelength, spectrum half
The described human skin of laser irradiation of the high width less than 1 nanometer, and control described human skin to form over
The illumination of 300000 Luxs, the biological evidence vestige of human skin described under light cutoff filter mirror under 540 nanometers
Show.
Preferably, after human skin being soaked or sprayed using described bioluminescence visualization reagent, human body is treated
After skin surface is dried, then described laser is used to irradiate.
Wherein, it is described to treat that human skin dries the reagent volatilization for referring to human skin, it can specifically use and add
Heat volatilization is volatilized naturally.
It is further preferred that treat that described human skin dries in the environment of no more than 50 DEG C.
More preferably, treat that described human skin dries in the environment of no more than 45 DEG C.
It is further preferable that treat that described human skin dries in the environment of no more than 40 DEG C.
Most preferably, treat that described human skin dries at 20 ~ 37 DEG C.
In the present invention, described human body includes corpse and live body.
Specifically, when described human body is live body, described human skin is put in described biological evidence
The described immersion of bioluminescence visualization reagent or spraying are carried out in 1 hour afterwards.
The present invention the principle that shows be:The self amino acid content of human skin is substantially consistent, and works as people
After body skin surface is locally pressed by other people, the position can be superimposed with other people amino acid, so as to cause total amino acid content high
In other positions, the present invention utilizes above-mentioned discovery, passes through the adjustment to bioluminescence visualization reagent so that human body self amino acid with
It will not show under laser irradiation after bioluminescence visualization reagent reaction, and the high position of amino acid content can show, so as to
Realize showing for human skin's biological evidence vestige.
For corpse, after people's death, metabolism stops, and the amino acid content change of human body surface is smaller,
Now, other positions can be higher than all the time by being superimposed with the amino acid content at the position of his human amino acid, and therefore, of the invention is biological glimmering
The biological evidence vestige that light visualization reagent can be used on the corpse of killed rear long period shows.
For live body, because human body carries out metabolism always, therefore, the position of his human amino acid is superimposed with
Amino acid content can be with the progress of itself metabolism, and tends to be consistent with the amino acid content at other positions, therefore, for
, it is necessary to carry out showing for biological evidence vestige in 1 hour for live body, if overlong time, biological evidence vestige can be caused
It can not show.
Third object of the present invention is to provide a kind of described bioluminescence visualization reagent in human skin's biology thing
Application in card the showing of vestige.
In the present invention, described human body includes corpse and live body.
Due to the implementation of above technical scheme, the present invention has the following advantages that compared with prior art:
The present invention can show the biological evidence trace of human skin by the adjustment to bioluminescence visualization reagent formula rate
Mark, consequently facilitating the extraction of inhereditary material, further, it is possible to show the streakline of fingerprint or the lines of the hand, consequently facilitating carrying out fingerprint contrast;
Also, the present invention, without the humidity of control object and environment, on the one hand simplifies the side of showing without being dried at high temperature
Face, on the other hand avoid the destruction to biological evidence on object.
Brief description of the drawings
Accompanying drawing 1 is the fingerprint of embodiment 1;
Accompanying drawing 2 is the fingerprint of embodiment 2;
Accompanying drawing 3 is the fingerprint of embodiment 3;
Accompanying drawing 4 is the partial enlarged drawing of the fingerprint of embodiment 3;
Accompanying drawing 5 is the fingerprint of embodiment 4;
Accompanying drawing 6 is the fingerprint of comparative example 1;
Accompanying drawing 7 is the fingerprint of comparative example 2.
Embodiment
With reference to specific embodiment, the present invention will be further described in detail, but is not limited to these embodiments.
Embodiment 1
(1), bioluminescence visualization reagent configuration:3g glycerine is added in 23g absolute alcohols, is sufficiently stirred dissolving, obtained solution 1;
0.003g indandione rodenticides are substantially dissolved in 2g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
71.997g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), using step(1)The bioluminescence visualization reagent of preparation sprays to cadaver skin surface, then in room temperature
Under(25℃)It is lower wait attach reagent volatilization after, with wavelength be 532 nanometers, spectrum halfwidth degree less than 1 nanometer laser irradiate
Corpse, and control corpse surface to form the illumination of 300,000 Luxs, obtained under 540 nanometers under light cutoff filter mirror by photographic means
Obtain finger mark and see Fig. 1.
Embodiment 2
(1), bioluminescence visualization reagent configuration:4g glycerine is added in 23g absolute alcohols, is sufficiently stirred dissolving, obtained solution 1;
0.001g indandione rodenticides are substantially dissolved in 1.5g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
71.499g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), other people hand be pressed against on the ankle of live body, then remove;After spending 10 minutes, using step(1)Prepare
Bioluminescence visualization reagent the pressing position is sprayed, then at room temperature(30℃)After lower wait attaches reagent volatilization,
It it is 532 nanometers with wavelength, spectrum halfwidth degree irradiates the position less than 1 nanometer of laser, and control the portion faces to form 30
The illumination of ten thousand Luxs, Fig. 2 is shown in by photographic means acquisition finger mark under light cutoff filter mirror under 540 nanometers.
Embodiment 3
(1), bioluminescence visualization reagent configuration:4g glycerine is added in 23g absolute alcohols, is sufficiently stirred dissolving, obtained solution 1;
0.005g indandione rodenticides are substantially dissolved in 2.5g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
70.495g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), other people hand be pressed against on the arm of live body, then remove;After spending 30 minutes, using step(1)Prepare
Bioluminescence visualization reagent the pressing position is sprayed, then at 40 DEG C wait attach reagent volatilization after, be with wavelength
532 nanometers, spectrum halfwidth degree irradiates the position less than 1 nanometer of laser, and controls the portion faces to form 300,000 Luxs
Illumination, under 540 nanometers under light cutoff filter mirror by photographic means obtain finger mark see Fig. 3, enlarged drawing is shown in Fig. 4.
Embodiment 4
(1), bioluminescence visualization reagent configuration:5g glycerine is added in 20g absolute alcohols, is sufficiently stirred dissolving, obtained solution 1;
0.001g indandione rodenticides are substantially dissolved in 3g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
71.999g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), other people hand be pressed against on the calf of live body, then remove;After spending 50 minutes, using step(1)Match somebody with somebody
The bioluminescence visualization reagent of system sprays to the pressing position, is then waited at 40 DEG C after attaching reagent volatilization, uses wavelength
The position is irradiated, and controls the portion faces to form 30 Wan Leke for 532 nanometers, laser of the spectrum halfwidth degree less than 1 nanometer
The illumination of department, Fig. 5 is shown in by photographic means acquisition finger mark under light cutoff filter mirror under 540 nanometers.
Comparative example 1
(1), bioluminescence visualization reagent configuration:6g glycerine is added in 30g absolute alcohols, is sufficiently stirred dissolving, obtained solution 1;
0.0008g indandione rodenticides are substantially dissolved in 0.1g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
63.8992g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), other people hand be pressed against on the calf of live body, then remove;After spending 5 minutes, using step(1)Prepare
Bioluminescence visualization reagent the pressing position is sprayed, then at room temperature(30℃)After lower wait attaches reagent volatilization,
It it is 532 nanometers with wavelength, spectrum halfwidth degree irradiates the position less than 1 nanometer of laser, and control the portion faces to form 30
The illumination of ten thousand Luxs, Fig. 6 is shown in by photographic means acquisition finger mark under light cutoff filter mirror under 540 nanometers.
Comparative example 2
(1), bioluminescence visualization reagent configuration:1.5g glycerine is added in 15g absolute alcohols, is sufficiently stirred dissolving, obtained solution
1;0.01g indandione rodenticides are substantially dissolved in 4g ethyl acetate, obtained solution 2;After solution 1 and solution 2 are mixed, add
79.49g petroleum ethers, stirring and dissolving, it is configured to bioluminescence visualization reagent.
(2), other people hand be pressed against on live body calf, then remove;After spending 10 minutes, using step(1)Prepare
Bioluminescence visualization reagent the pressing position is sprayed, then at room temperature(30℃)After lower wait attaches reagent volatilization,
It it is 532 nanometers with wavelength, spectrum halfwidth degree irradiates the position less than 1 nanometer of laser, and control the portion faces to form 30
The illumination of ten thousand Luxs, Fig. 7 is shown in by photographic means acquisition finger mark under light cutoff filter mirror under 540 nanometers.
The present invention is described in detail above, its object is to allow the personage for being familiar with this art to understand this
The content of invention is simultaneously carried out, and it is not intended to limit the scope of the present invention, all Spirit Essence institutes according to the present invention
The equivalent change or modification of work, it should all cover within the scope of the present invention.
Claims (10)
- A kind of 1. bioluminescence visualization reagent for being used to show human skin's biological evidence vestige, it is characterised in that:By described The quality of bioluminescence visualization reagent be 100% meter, the composition of raw materials of described bioluminescence visualization reagent is:Indandione rodenticide 0.001% ~ 0.005%, ethyl acetate 1% ~ 3%, glycerine 2% ~ 5%, absolute alcohol 20% ~ 25%, petroleum ether 67% ~ 72%.
- A kind of 2. process for show for showing human skin's biological evidence vestige, it is characterised in that:Using described in claim 1 Bioluminescence visualization reagent human skin is soaked or sprayed, be then 532 nanometers, spectrum halfwidth degree with wavelength The human skin described in laser irradiation less than 1 nanometer, and control described human skin to form over 300,000 Le The illumination of gram department, the biological evidence vestige of human skin described under light cutoff filter mirror can show under 540 nanometers.
- 3. process for show according to claim 2, it is characterised in that:Using described bioluminescence visualization reagent to human body skin After skin surface is soaked or sprayed, after human skin dries, then described laser is used to irradiate.
- 4. process for show according to claim 3, it is characterised in that:Described human body is treated in the environment of no more than 50 DEG C Skin surface is dried.
- 5. process for show according to claim 4, it is characterised in that:Described human body is treated in the environment of no more than 45 DEG C Skin surface is dried.
- 6. process for show according to claim 5, it is characterised in that:Described human body is treated in the environment of no more than 40 DEG C Skin surface is dried.
- 7. the process for show according to any one of claim 2 to 6, it is characterised in that:Described human body include corpse and Live body.
- 8. process for show according to claim 7, it is characterised in that:When described human body is live body, in described life Thing material evidence carries out the described immersion of bioluminescence visualization reagent or spraying after putting on described human skin in 1 hour.
- 9. a kind of bioluminescence visualization reagent as claimed in claim 1 is in the showing of human skin's biological evidence vestige Using.
- 10. application according to claim 9, it is characterised in that:Described human body includes corpse and live body.
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CN201710785697.1A CN107582067B (en) | 2017-09-04 | 2017-09-04 | Biological fluorescence developing agent for developing biological material evidence trace on human skin surface and developing method |
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CN201710785697.1A CN107582067B (en) | 2017-09-04 | 2017-09-04 | Biological fluorescence developing agent for developing biological material evidence trace on human skin surface and developing method |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109199399A (en) * | 2018-09-05 | 2019-01-15 | 苏州纳磐新材料科技有限公司 | Fingerprint manifestation agent and preparation method thereof |
CN112754477A (en) * | 2019-11-05 | 2021-05-07 | 南京大学 | Latent fingerprint display method based on cesium halide lead perovskite nano material |
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CN106802292A (en) * | 2017-01-22 | 2017-06-06 | 苏州晓松科技开发有限公司 | A kind of method for manifesting permeability object biological evidence vestige |
CN106841140A (en) * | 2017-01-22 | 2017-06-13 | 苏州晓松科技开发有限公司 | A kind of biological evidence vestige manifests extracting method |
CN106872437A (en) * | 2017-04-14 | 2017-06-20 | 南京工业大学 | A kind of fingerprint based on MOFs presomas quickly manifests reagent and its application |
CN106974659A (en) * | 2017-05-20 | 2017-07-25 | 复旦大学 | A kind of latent fingerprint detection method based on red fluorescence carbon point material |
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US4236082A (en) * | 1979-01-29 | 1980-11-25 | Palmguard, Inc. | Method and apparatus for recording image details of the palm of a hand |
WO1988001730A1 (en) * | 1986-08-27 | 1988-03-10 | The Australian National University | Method and apparatus for distinguishing photoluminescent and reflecting surfaces in forensic science applications |
WO2009067761A1 (en) * | 2007-11-29 | 2009-06-04 | University Of Technology, Sydney | A method of developing latent fingerprints |
CN105105761A (en) * | 2015-09-16 | 2015-12-02 | 福建师范大学 | Chemical appearing method for potential finger prints |
CN106802292A (en) * | 2017-01-22 | 2017-06-06 | 苏州晓松科技开发有限公司 | A kind of method for manifesting permeability object biological evidence vestige |
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CN106872437A (en) * | 2017-04-14 | 2017-06-20 | 南京工业大学 | A kind of fingerprint based on MOFs presomas quickly manifests reagent and its application |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109199399A (en) * | 2018-09-05 | 2019-01-15 | 苏州纳磐新材料科技有限公司 | Fingerprint manifestation agent and preparation method thereof |
CN109199399B (en) * | 2018-09-05 | 2021-11-26 | 苏州纳磐新材料科技有限公司 | Fingerprint developing agent and preparation method thereof |
CN112754477A (en) * | 2019-11-05 | 2021-05-07 | 南京大学 | Latent fingerprint display method based on cesium halide lead perovskite nano material |
CN112754477B (en) * | 2019-11-05 | 2021-12-24 | 南京大学 | Latent fingerprint display method based on cesium halide lead perovskite nano material |
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