CN107576808B - It is a kind of quickly to detect the remaining device and method of pesticide in fruit and vegetable food - Google Patents
It is a kind of quickly to detect the remaining device and method of pesticide in fruit and vegetable food Download PDFInfo
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Abstract
The invention discloses the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food, mainly it is made of the sample solution to be tested preparation facilities, subpackage apparatus, transmission device, the sample solution to be tested storing unit, turntable and check analysis device, the sample solution to be tested preparation facilities is connected to subpackage apparatus by pipe fitting, the sample solution to be tested is divided in the sample solution to be tested storing unit, turntable is sent to by transmission device again, check analysis device is located at the right side of turntable, and inside is equipped with optical detection apparatus described in optical detection apparatus and host and is connected to host by call wire.The present invention collects crushing, extraction, purifying, sample liquid preparation, sample liquid packing, sample liquid transmission and sample liquid and is detected on one, high degree of automation, it is convenient to operate, fast and effeciently the pesticide residue on sample to be tested can be detected, and testing result is more accurate, it can also be carried out continuously the Detecting Pesticide of multiple and different samples to be tested in a short time, it is high-efficient.
Description
Technical field
The present invention relates to pesticide residue detection device technical fields, are specifically related to a kind of quickly detection fruit and vegetable food middle peasant
The remaining device and method of medicine.
Background technique
China is large agricultural country, and the production and use of pesticide are very universal extensively in China, especially highly toxic organic
Phosphorus and carbamate chemicals for agriculture are that China produces and the maximum pesticide of usage amount at present.The large area of pesticide is used and is abused,
In addition shortage is not in place to supervising caused by pesticide fast and effectively on-site test means in society, so that China vegetables, water
The exceeded phenomenon of remains of pesticide is quite serious in the agricultural product such as fruit, while also resulting in pesticide to China's surface water and groundwater
Pollution.On the other hand, with the continuous improvement of our people's living standard, people increasingly pay close attention to safe diet problem.Herein
In the case of, research and development can be to the side that the scene of carrying out of pesticide contained by fruit and vegetable food remains of pesticide and water body is quick and precisely detected
Method and instrument have highly important social effect and practical value.
Currently, the sample-pretreating method of Detecting Pesticide is usually and presses in national standard (GB/T5009.199-2003)
Step carries out, process are as follows: sampling crushes, extraction, purification.If the process is all carried out using artificial, relatively time consuming.So
And subsequent enzyme inhibition detects pesticide residue process Highgrade integration, detects sample number per hour and (picks up to 300~400
Except the pre-treatment time).The whole efficiency of these high-throughput detection devices is played, only increases personnel in preceding processing link,
Shorten the pre-treatment time, can be only achieved whole efficient purpose.It should say, the pre-treatment link of fruit and vegetable food has become the residual inspection of agriculture
The rate-limiting step of survey.
Outside woods, mainly method is used in conjunction to vegetables, water using gas chromatography, liquid chromatography and gas chromatography-mass spectrum in China
Contained pesticide is detected in remains of pesticide and water body in the agricultural product such as fruit.Although these large-scale instrument high sensitivities, detection
As a result accurate and reliable, but there are instrument price valuableness, and complicated for operation, sample needs pre-treatment, and testing cost is high, and detection time is long,
The disadvantages of being not suitable for field quick detection.
Therefore need to design it is a kind of being capable of fast automatic processing sample to be tested and the more accurate equipment and side of testing result
Method.
Summary of the invention
The technical problem to be solved by the present invention is to provide can automatically process sample to be tested and testing result is more accurate
The quickly remaining device and method of pesticide in detection fruit and vegetable food.
In order to solve the above technical problems, the technical solution adopted in the present invention is as follows:
It is a kind of quickly to detect the remaining equipment of pesticide in fruit and vegetable food, mainly filled by the sample solution to be tested preparation facilities, packing
It sets, transmission device, the sample solution to be tested storing unit, turntable and check analysis device composition, the sample solution to be tested preparation facilities includes
Microdisintegrator, micro centrifuge, miniature refiner, extractant storage device, macroporous absorbent resin adsorbent equipment, to test sample
Liquid preparation facilities power supply, buffer storage device, sample liquid device for formulating, the first shelf and the second shelf, first shelf position
In the left side of the sample solution to be tested preparation facilities position on the upper side, second shelf is located at the right side position on the lower side of the sample solution to be tested preparation facilities
It sets, the Microdisintegrator top is equipped with Microdisintegrator end cap, and lower part is equipped with Microdisintegrator motor, and Microdisintegrator is placed in
On first shelf, Microdisintegrator pass through the sample solution to be tested preparation facilities upper surface, make the Microdisintegrator end cap be located to
On the upper surface of sample measuring liquid preparation facilities, the micro centrifuge, miniature refiner, macroporous absorbent resin adsorbent equipment are from a left side
It is sequentially placed into the right on second shelf, the extractant storage device is located at the top of miniature refiner, extractant
Storage device top passes through the sample solution to be tested preparation facilities, and the extractant storage device top cover of upper part is made to be located at the sample solution to be tested system
On the upper surface of standby device, centrifuge tube is equipped in the micro centrifuge, bottom is equipped with micro centrifuge motor, described miniature
It is homogenized motor spindle and is equipped with miniature refiner motor, the Microdisintegrator is connected to the micro centrifuge, institute by conduit one
It states extractant storage device and micro centrifuge is connected to by extract liquor ingress pipe, micro centrifuge is connected to by conduit two
The miniature refiner, is equipped with micropump on the conduit two, and the miniature refiner is connected to described big by conduit three
Macroporous adsorbent resin adsorbent equipment, the sample liquid device for formulating, buffer storage device and the equal position of the sample solution to be tested preparation facilities power supply
In the lower section of the second shelf, and sample liquid device for formulating, buffer storage device and the sample solution to be tested preparation facilities power supply are from right to left
It is sequentially placed, the bottom of macroporous absorbent resin adsorbent equipment is connect by conduit five with sample liquid device for formulating, sample liquid device for formulating
It is connect between buffer storage device by conduit four, the Microdisintegrator motor, micro centrifuge motor, miniature homogenate
The motor of electromechanical machine and sample liquid device for formulating passes through conducting wire and is connected to the sample solution to be tested preparation facilities power supply, and the sample liquid is prepared
Device right end is equipped with conduit six, and the conduit six is pierced by the right side of the sample solution to be tested preparation facilities from right side and is connected by pipe fitting
It is connected to the subpackage apparatus, the sample solution to be tested is divided in the sample solution to be tested storing unit by subpackage apparatus, it is described to be measured
Sample liquid storing unit passes sequentially through the transmission device and is sent to the turntable, and the check analysis device is located at the right side of turntable
Side, check analysis device left side are equipped with mechanical arm, and inside is equipped with optical detection apparatus and host, and optical detection apparatus is optics biography
Feel detector, is sent the sample solution to be tested storing unit on turntable to the optical detection apparatus by the mechanical arm, it is described
Optical detection apparatus is connected to the host by call wire.
Further, in the above scheme, the extractant storage device is divided into three parts, stores and fills in extractant
Top set, which covers, is equipped with extractant dosing mouth corresponding with the three parts, the extract liquor ingress pipe point three tunnels respectively with institute
It states three parts to be connected, different extractants can be added in three parts, when continuously detecting multiple samples, be convenient for basis
Different extractions are selected, and the wash number to extractant storage device is reduced.
Further, in the above scheme, backwash water tank is additionally provided in the sample solution to be tested preparation facilities, it is described anti-
Flush box is connected to the conduit six by backwash tube, is equipped with three in the place that the backwash tube is connected with conduit six
Port valve can use water in backwash water tank to the Microdisintegrator, micro centrifuge, micro- in entire sample measuring liquid preparation facilities
Type refiner, macroporous absorbent resin adsorbent equipment and sample liquid device for formulating are backwashed, and backwash tube road can be set
Circulating pump, backwash water outlet can export to come from Microdisintegrator end cap.
Further, the transmission device bottom is equipped with rack, the also external transmission of the transmission device in the above scheme
It is not shown on device driving motor figure.
Further, in the above scheme, bar code is posted on the side of the sample solution to be tested storing unit.
Further, in the above scheme, it is additionally provided with RFID bar code reading device on the check analysis device, passes through
RFID bar code reading device reads bar code information, can be convenient for detection data progress calculation processing.
Further, in the above scheme, the mechanical arm includes flexing transverse arm and vertical telescopic arm, and the transverse direction is stretched
Contracting arm is mounted on the upper left side of check analysis device, and the vertical telescopic arm is mounted below the left end of flexing transverse arm and vertical
In flexing transverse arm, it is equipped with mechanical gripper in the bottom of vertical telescopic arm, the mechanical arm is by being located at check analysis device
Interior telescopic arm controls motor to control, and the right side of the telescopic arm control motor is equipped with check analysis installation's power source, telescopic arm
It controls motor and is connected to optical detection apparatus and be connected to the check analysis installation's power source.
The method for carrying out Detecting Pesticide using the remaining equipment of pesticide in described one kind quickly detection fruit and vegetable food,
The following steps are included:
S1: opening the sample solution to be tested preparation facilities power supply, is the Microdisintegrator electricity by the sample solution to be tested preparation facilities power supply
Machine power supply, sample to be tested is placed in the Microdisintegrator, is crushed by Microdisintegrator, while molten by extracting
Agent dosing mouth pours into extractant into the extractant storage device；
Smashed sample to be tested: being directed into micro centrifuge by S2 by conduit one, will be extracted by extract liquor ingress pipe
Extract liquor in solvent storage device is also introduced into micro centrifuge, by the sample solution to be tested preparation facilities power supply be it is described it is miniature from
The power supply of scheming motor is opened micro centrifuge and is centrifuged, centrifugal speed 800-1000r/min, centrifugation time 5-
20min；
S3: the supernatant in micro centrifuge is sucked into miniature homogenate by the micropump being arranged by conduit two and thereon
Machine is powered for the miniature refiner motor by the sample solution to be tested preparation facilities power supply, opens miniature refiner and carry out homogenate extraction,
Obtain extract liquor；
Extract liquor: being directed into macroporous absorbent resin adsorbent equipment by conduit three and carry out absorption purification by S4, is obtained net
Extract liquor after change；
S5: purified extract liquor is directed by sample liquid device for formulating by conduit five, while will be buffered by conduit four
Buffer in liquid storage device is also introduced into sample liquid device for formulating, is equipped with flow control on the conduit four and conduit five
Valve is sample liquid preparation by the sample solution to be tested preparation facilities power supply for controlling the ratio of purified extract liquor and buffer
The motor of device is powered, and purified extract liquor is mixed evenly in proportion with buffer, and preparation obtains the sample solution to be tested；
S6: the sample solution to be tested is sent by conduit six to subpackage apparatus, the sample solution to be tested is divided in the sample solution to be tested storing unit
It is interior；Bar code is sticked in the side of the sample solution to be tested storing unit by the operation of artificial or machine；
S7: the sample solution to be tested storing unit after sticking bar code is sent to turntable by device is sequentially transmitted；
S8: the sample solution to be tested storing unit on turntable is sent to optical detection apparatus by the mechanical gripper of mechanical arm bottom
Interior, optical detection apparatus tests and analyzes the sample solution to be tested in the sample solution to be tested storing unit, and the data that will test pass through
Call wire is transmitted to host；
S9: CPU, data storage cell, data analysis unit and data processing unit, the data storage are equipped in host
The data that unit detects optical detection apparatus store, and are sent to the data analysis unit, to detected
Data are analyzed, and then the data processing unit are being sent to, by data processing unit by the data measured with every 10-
30 groups of data are that unit calculates its average value, and obtained average value is exported as testing result.
Further, the triple valve is opened, using the water in backwash water tank in entire sample measuring liquid preparation facilities
Microdisintegrator, micro centrifuge, miniature refiner, macroporous absorbent resin adsorbent equipment and sample liquid device for formulating carry out anti-
It rinses, circulating pump can be set in backwash tube road, and backwash water outlet can export to come from Microdisintegrator end cap, and recoil washes
Cheng Hou can carry out the detection of next sample to be tested.
The beneficial effects of the present invention are: the present invention collects crushing, extraction, purifying, the sample liquid preparation, sample liquid of sample to be tested
Packing, sample liquid transmission and sample liquid are detected on one, and high degree of automation, it is convenient to operate, can be fast and effeciently to sample to be tested
On pesticide residue detected, and the data that will be measured are by check analysis device of the invention with every 10-30 group data
Unit calculates its average value, and obtained average value is exported as testing result, and error can be effectively reduced, and ties detection
Fruit is more accurate, in addition, the present invention has backwashing function, can be carried out continuously multiple and different samples to be tested in a short time
Detecting Pesticide, it is high-efficient.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of the sample solution to be tested preparation facilities；
Wherein, 1- the sample solution to be tested preparation facilities, 2- subpackage apparatus, 3- transmission device, 4- the sample solution to be tested storing unit, 5- turn
Disk, 6- check analysis device, 11- Microdisintegrator, 111- Microdisintegrator end cap, 112- Microdisintegrator motor, 113- are led
The first shelf of Guan Yi, 114-, the second shelf of 115-, 12- micro centrifuge, 121- centrifuge tube, 122- conduit two, 123- extract liquor
Ingress pipe, 124- micropump, 125- micro centrifuge motor, the miniature refiner of 13-, 131- conduit three, the miniature refiner of 132-
Motor, 14- extractant storage device, 141- extractant storage device top cover, 142- extractant dosing mouth, 15- macropore
Adsorb resin adsorption device, 16- the sample solution to be tested preparation facilities power supply, 17- buffer storage device, 171- conduit four, 18- sample liquid
Device for formulating, 181- conduit five, 182- conduit six, 19- backwash water tank, 191- backwash tube, 192- triple valve, 20- pipe connect
Head, 31- rack, 51- rotary base, 61- mechanical arm, 611- flexing transverse arm, the vertical telescopic arm of 612-, 62- mechanical gripper,
63- telescopic arm control motor, 64- check analysis installation's power source, 65- optical detection apparatus, 66- call wire, 67- host, 68- are opened
Hole.
Specific embodiment
In the following with reference to the drawings and specific embodiments come to the present invention be further described in detail.
One kind as shown in Figure 1 quickly detects the remaining equipment of pesticide in fruit and vegetable food, is mainly prepared by the sample solution to be tested
Device 1, subpackage apparatus 2, transmission device 3, the sample solution to be tested storing unit 4, turntable 5 and check analysis device 6 form, the sample solution to be tested
Preparation facilities 1 includes Microdisintegrator 11, micro centrifuge 12, miniature refiner 13, extractant storage device 14, macropore suction
Attached resin adsorption device 15, the sample solution to be tested preparation facilities power supply 16, buffer storage device 17, sample liquid device for formulating 18, first
Shelf 114 and the second shelf 115, the first shelf 114 are located at the left side position on the upper side of the sample solution to be tested preparation facilities 1, the second shelf
115 are located at the right side position on the lower side of the sample solution to be tested preparation facilities 1, and 11 top of Microdisintegrator is equipped with Microdisintegrator end cap 111,
Lower part is equipped with Microdisintegrator motor 112, and Microdisintegrator 11 is placed on the first shelf 114, and Microdisintegrator 11 passes through to be measured
The upper surface of sample liquid preparation facilities 1 is located at Microdisintegrator end cap 111 on the upper surface of the sample solution to be tested preparation facilities 1, micro-
Type centrifuge 12, miniature refiner 13, macroporous absorbent resin adsorbent equipment 15 are sequentially placed into from left to right on the second shelf 115,
Extractant storage device 14 is located at the top of miniature refiner 13, and 14 top of extractant storage device passes through the sample solution to be tested system
Standby device 1, is located at the extractant storage device top cover 141 of upper part on the upper surface of the sample solution to be tested preparation facilities 1, micro-
Centrifuge tube 121 is equipped in type centrifuge 12, bottom is equipped with micro centrifuge motor 125, and miniature 13 bottom of refiner is equipped with miniature
Refiner motor 132, Microdisintegrator 11 are connected to micro centrifuge 12, extractant storage device 14 by conduit 1
It is connected to micro centrifuge 12 by extract liquor ingress pipe 123, micro centrifuge 12 is connected to miniature homogenate by conduit 2 122
Machine 13, is equipped with micropump 124 on conduit 2 122, and miniature refiner 13 is connected to macroporous absorbent resin suction by conduit 3 131
Adsorption device 15, sample liquid device for formulating 18, buffer storage device 17 and the sample solution to be tested preparation facilities power supply 16 are respectively positioned on second and put
The lower section of plate 115, and sample liquid device for formulating 18, buffer storage device 17 and the sample solution to be tested preparation facilities power supply 16 are from right to left
It is sequentially placed, the bottom of macroporous absorbent resin adsorbent equipment 15 is connect by conduit 5 181 with sample liquid device for formulating 18, and sample liquid is matched
It is connected between device 18 and buffer storage device 17 processed by conduit 4 171, Microdisintegrator motor 112, micro centrifuge
The motor of motor 125, miniature refiner motor 132 and sample liquid device for formulating 183 passes through conducting wire and is connected to the sample solution to be tested system
Standby installation's power source 16,18 right end of sample liquid device for formulating are equipped with conduit 6 182, and conduit 6 182 is pierced by the sample solution to be tested preparation from right side
The right side of device 1 and subpackage apparatus 2 is connected to by pipe fitting 20, is divided in the sample solution to be tested by subpackage apparatus 2 to be measured
In sample liquid storing unit 4, the sample solution to be tested storing unit 4 passes sequentially through transmission device 3 and is sent to turntable 5, and 6, check analysis device
Mechanical arm 61 is equipped on the left of the right side of turntable 5, check analysis device 6, inside is equipped with optical detection apparatus 65 and host 67, light
Detection device 65 is optical sensing detector；The sample solution to be tested storing unit 4 on turntable 5 is sent to optics by mechanical arm 61
In detection device 65, optical detection apparatus 65 is connected to host 67 by call wire 66.
Wherein, extractant storage device 14 divides for three parts, is equipped on extractant storage device top cover 141 and three
The corresponding extractant dosing mouth 142 in part, extract liquor ingress pipe 123 divide three tunnels to be connected respectively with three parts, Ke Yi
Different extractants is added in three parts, when continuously detecting multiple samples, convenient for being selected according to different extractions
It selects, reduces the wash number to extractant storage device 14.
Backwash water tank 19 is equipped in the sample solution to be tested preparation facilities 1, backwash water tank 19 is connected by backwash tube 191
To conduit 6 182, it is equipped with triple valve 192 in the place that backwash tube 191 is connected with conduit 6 182, can use backwash
Water in water tank 19 in entire sample measuring liquid preparation facilities 1 Microdisintegrator 11, micro centrifuge 12, miniature refiner 13,
Macroporous absorbent resin adsorbent equipment 15 and sample liquid device for formulating 18 are backwashed, and circulation can be set in backwash tube road
Pump, backwash water outlet can export to come from Microdisintegrator end cap 111.
3 bottom of transmission device is equipped with rack 31, is not shown on the also external transmission device driving motor figure of transmission device 3.
Bar code is posted on the side of the sample solution to be tested storing unit 4.RFID bar code reading is additionally provided on check analysis device 6
Device is taken, bar code information is read by RFID bar code reading device, it can be convenient for detection data progress calculation processing.
Wherein, mechanical arm 61 includes flexing transverse arm 611 and vertical telescopic arm 612, and flexing transverse arm 611 is mounted on inspection
The upper left side of analytical equipment 6, vertical telescopic arm 612 are mounted below the left end of flexing transverse arm 611 and perpendicular to transversal stretching
Arm 611 is equipped with mechanical gripper 62 in the bottom of vertical telescopic arm 612, and mechanical arm 61 is by being located in check analysis device 6
Telescopic arm controls motor 63 to control, and the right side that telescopic arm controls motor 63 is equipped with check analysis installation's power source 64, telescopic arm control
It motor processed 63 and is connected to optical detection apparatus 65 and is connected to check analysis installation's power source 64.
The method for carrying out Detecting Pesticide using the remaining equipment of pesticide in above-mentioned one kind quickly detection fruit and vegetable food,
The following steps are included:
S1: opening the sample solution to be tested preparation facilities power supply 16, is Microdisintegrator electricity by the sample solution to be tested preparation facilities power supply 16
Machine 112 is powered, and sample to be tested is placed in Microdisintegrator 11, is crushed by Microdisintegrator 11, while passing through extraction
Solvent dosing mouth 142 is taken to pour into extractant into extractant storage device 14；
S2: smashed sample to be tested is directed into micro centrifuge 12 by conduit 1, by extract liquor ingress pipe
123 are also introduced into the extract liquor in extractant storage device 14 to micro centrifuge 12, by the sample solution to be tested preparation facilities electricity
Source 16 is the power supply of micro centrifuge motor 125, opens micro centrifuge 12 and is centrifuged, centrifugal speed 800-1000r/min,
Centrifugation time is 5-20min；
S3: the supernatant in micro centrifuge 12 is sucked into micro- by the micropump 124 being arranged by conduit 2 122 and thereon
Type refiner 13, by the sample solution to be tested preparation facilities power supply 16 be miniature refiner motor 132 power, open miniature refiner 13 into
Row homogenate extraction, obtains extract liquor；
S4: extract liquor is directed into macroporous absorbent resin adsorbent equipment 15 by conduit 3 131 and carries out absorption purification, is obtained
To purified extract liquor；
S5: purified extract liquor is directed by sample liquid device for formulating 18 by conduit 5 181, while passing through conduit four
171 are also introduced into the buffer in buffer storage device 17 to sample liquid device for formulating 18, in conduit 4 171 and conduit 5 181
On be equipped with flow control valve, for controlling the ratio of purified extract liquor and buffer, by the sample solution to be tested preparation facilities electricity
Source 16 is that the motor of sample liquid device for formulating 18 is powered, and purified extract liquor is mixed evenly in proportion with buffer, is matched
The sample solution to be tested is made；
S6: the sample solution to be tested is sent by conduit 6 182 to subpackage apparatus 2, and the sample solution to be tested is divided in the sample solution to be tested storage
In device 4；Bar code is sticked in the side of the sample solution to be tested storing unit 4 by the operation of artificial or machine；
S7: the sample solution to be tested storing unit 4 after sticking bar code is sequentially transmitted device 3 and is sent to turntable 5；
S8: the sample solution to be tested storing unit 4 on turntable 5 is sent to optics by the mechanical gripper 62 of 61 bottom of mechanical arm and is examined
In measurement equipment 65, optical detection apparatus 65 tests and analyzes the sample solution to be tested in the sample solution to be tested storing unit 4, and will test
Data host 67 is transmitted to by call wire 66；
S9: being equipped with CPU, data storage cell, data analysis unit and data processing unit in host 67, data storage is single
Optical detection apparatus 65 is detected obtained data and stored by member, and is sent to data analysis unit, to data detected
It is analyzed, is then being sent to data processing unit, by data processing unit by the data measured with every 10-30 group data
Its average value is calculated for unit, obtained average value is exported as testing result；
S10: opening triple valve 192, using the water in backwash water tank 19 to miniature in entire sample measuring liquid preparation facilities 1
Crusher 11, micro centrifuge 12, miniature refiner 13, macroporous absorbent resin adsorbent equipment 15 and sample liquid device for formulating 18
It is backwashed, circulating pump can be set in backwash tube road, and backwash water outlet can be exported from Microdisintegrator end cap 111
Come, after the completion of backwash, the detection of next sample to be tested can be carried out.
The crushing of this equipment collection sample to be tested, extraction, purifying, sample liquid is prepared, sample liquid packing, sample liquid transmits and sample liquid
It is detected on one, high degree of automation, it is convenient to operate, and can fast and effeciently examine to the pesticide residue on sample to be tested
It surveys, and the data that will be measured are calculated its average value, institute by check analysis device of the invention as unit of every 10-30 group data
Obtained average value is exported as testing result, and error can be effectively reduced, and keeps testing result more accurate, in addition, this
Invention has backwashing function, can be carried out continuously the Detecting Pesticide of multiple and different samples to be tested, efficiency in a short time
It is high.
Compliance test result: Detecting Pesticide is carried out to 15 kinds of fruit and vegetable foods using equipment of the invention, passes through testing result
It was found that equipment detection efficiency of the invention is high, and testing result accuracy is up to 98.3% or more.
The above is only the citing of embodiments of the present invention, it is noted that for the ordinary skill of the art
For personnel, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improve and become
Type also should be regarded as protection scope of the present invention.
Claims (8)
1. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food, which is characterized in that mainly prepared and filled by the sample solution to be tested
Set (1), subpackage apparatus (2), transmission device (3), the sample solution to be tested storing unit (4), turntable (5) and check analysis device (6) group
At the sample solution to be tested preparation facilities (1) includes Microdisintegrator (11), micro centrifuge (12), miniature refiner (13), extraction
Solvent storage device (14), macroporous absorbent resin adsorbent equipment (15), the sample solution to be tested preparation facilities power supply (16), buffer is taken to deposit
Storage device (17), sample liquid device for formulating (18), the first shelf (114) and the second shelf (115), the first shelf (114) position
In the left side position on the upper side of the sample solution to be tested preparation facilities (1), second shelf (115) is located at the sample solution to be tested preparation facilities (1)
Right side position on the lower side, Microdisintegrator (11) top is equipped with Microdisintegrator end cap (111), and lower part is equipped with miniature broken
Electromechanical machine (112), Microdisintegrator (11) are placed on the first shelf (114), and Microdisintegrator (11) is prepared across the sample solution to be tested
The upper surface of device (1) is located at the Microdisintegrator end cap (111) on the upper surface of the sample solution to be tested preparation facilities (1),
The micro centrifuge (12), miniature refiner (13), macroporous absorbent resin adsorbent equipment (15) are sequentially placed into institute from left to right
It states on the second shelf (115), the extractant storage device (14) is located at the top of miniature refiner (13), and extractant is deposited
Storage device (14) top passes through the sample solution to be tested preparation facilities (1), is located at the extractant storage device top cover (141) of upper part
On the upper surface of the sample solution to be tested preparation facilities (1), centrifuge tube (121) are equipped in the micro centrifuge (12), bottom is equipped with
Micro centrifuge motor (125), miniature refiner (13) bottom is equipped with miniature refiner motor (132), described miniature broken
Broken machine (11) is connected to the micro centrifuge (12) by conduit one (113), and the extractant storage device (14) passes through
Extract liquor ingress pipe (123) is connected to micro centrifuge (12), and micro centrifuge (12) is connected to described by conduit two (122)
Miniature refiner (13), is equipped with micropump (124) on the conduit two (122), and the miniature refiner (13) passes through conduit
Three (131) are connected to the macroporous absorbent resin adsorbent equipment (15), the sample liquid device for formulating (18), buffer storage device
(17) and the sample solution to be tested preparation facilities power supply (16) is respectively positioned on the lower sections of the second shelf (115), and sample liquid device for formulating (18), slow
Fliud flushing storage device (17) and the sample solution to be tested preparation facilities power supply (16) are sequentially placed from right to left, macroporous absorbent resin absorption dress
The bottom for setting (15) is connect by conduit five (181) with sample liquid device for formulating (18), and sample liquid device for formulating (18) is deposited with buffer
It is connected between storage device (17) by conduit four (171), the Microdisintegrator motor (112), micro centrifuge motor
(125), the motor of miniature refiner motor (132) and sample liquid device for formulating (18) passes through conducting wire and is connected to the sample solution to be tested system
Standby installation's power source (16), sample liquid device for formulating (18) right end are equipped with conduit six (182), and the conduit six (182) is from right side
It is pierced by the right side of the sample solution to be tested preparation facilities (1) and is connected to the subpackage apparatus (2) by pipe fitting (20), pass through packing
The sample solution to be tested is divided in the sample solution to be tested storing unit (4) by device (2), and the sample solution to be tested storing unit (4) is successively
It is sent to the turntable (5) by the transmission device (3), the check analysis device (6) is located at the right side of turntable (5), inspection
It tests on the left of analytical equipment (6) and is equipped with mechanical arm (61), inside is equipped with optical detection apparatus (65) and host (67), passes through the machine
Tool arm (61) send the sample solution to be tested storing unit (4) on turntable (5) to the optical detection apparatus (65), the optics inspection
Measurement equipment (65) is connected to the host (67) by call wire (66).
2. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 1, which is characterized in that described
Extractant storage device (14) is divided into three parts, is equipped with and the three parts on extractant storage device top cover (141)
Corresponding extractant dosing mouth (142), three tunnels of the extract liquor ingress pipe (123) point are connected with the three parts respectively
It is logical.
3. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 1, which is characterized in that in institute
It states and is additionally provided with backwash water tank (19) in the sample solution to be tested preparation facilities (1), the backwash water tank (19) passes through backwash tube
(191) it is connected to the conduit six (182), is equipped with three in the place that the backwash tube (191) is connected with conduit six (182)
Port valve (192).
4. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 1, which is characterized in that in institute
It states and posts bar code on the side of the sample solution to be tested storing unit (4).
5. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 4, which is characterized in that in institute
It states and is additionally provided with RFID bar code reading device on check analysis device (6).
6. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 4, which is characterized in that in institute
It states and is additionally provided with bar code reading device on check analysis device (6).
7. the remaining equipment of pesticide in a kind of quickly detection fruit and vegetable food according to claim 1, which is characterized in that described
Mechanical arm (61) includes flexing transverse arm (611) and vertical telescopic arm (612), and the flexing transverse arm (611) is mounted on inspection
The upper left side of analytical equipment (6), the vertical telescopic arm (612) are mounted below the left end of flexing transverse arm (611) and vertical
In flexing transverse arm (611), mechanical gripper (62) are equipped in the bottom of vertical telescopic arm (612), the mechanical arm (61) is logical
It crosses the telescopic arm being located in check analysis device (6) and controls motor (63) to control, the telescopic arm controls the right side of motor (63)
Side is equipped with check analysis installation's power source (64), and telescopic arm control motor (63) and optical detection apparatus (65) are connected to the inspection
Test analytical equipment power supply (64).
8. carrying out agriculture using the remaining equipment of pesticide in described one kind as claimed in any one of claims 1 to 6 quickly detection fruit and vegetable food
The method of medicine residue detection, which comprises the following steps:
S1: sample to be tested is placed in the Microdisintegrator (11), is crushed by Microdisintegrator (11), Xiang Suoshu
Extractant is poured into extractant storage device (14)；
S2: smashed sample to be tested is directed into micro centrifuge (12) by conduit one (113), by extract liquor ingress pipe
(123) extract liquor in extractant storage device (14) is also introduced into micro centrifuge (12), opens micro centrifuge
(12) it is centrifuged, centrifugal speed 800-1000r/min, centrifugation time is 5-20min or so；
S3: the supernatant in micro centrifuge (12) is sucked by the micropump (124) being arranged by conduit two (122) and thereon
Miniature refiner (13) opens miniature refiner (13) and carries out homogenate extraction, obtains extract liquor；
S4: extract liquor is directed into macroporous absorbent resin adsorbent equipment (15) by conduit three (131) and carries out absorption purification, is obtained
To purified extract liquor；
S5: purified extract liquor is directed by sample liquid device for formulating (18) by conduit five (181), while passing through conduit
(171) buffer in buffer storage device (17) is also introduced into sample liquid device for formulating (18), by purified extract liquor
It is prepared after being mixed in proportion with buffer and obtains the sample solution to be tested；
S6: the sample solution to be tested is sent by conduit six (182) to subpackage apparatus (2), and the sample solution to be tested is divided in the sample solution to be tested storage
In device (4)；Bar code is sticked in the side of the sample solution to be tested storing unit (4) by the operation of artificial or machine；
S7: the sample solution to be tested storing unit (4) after sticking bar code is sequentially delivered to turntable (5) by transmission device (3)；
S8: the sample solution to be tested storing unit (4) on turntable (5) is sent to light by the mechanical gripper (62) of mechanical arm (61) bottom
It learns in detection device (65), optical detection apparatus (65) carries out detection point to the sample solution to be tested in the sample solution to be tested storing unit (4)
Analysis, and the data that will test are transmitted to host (67) by call wire (66)；
S9: CPU, data storage cell, data analysis unit and data processing unit, the data storage are equipped in host (67)
Unit stores the obtained data of optical detection apparatus (65) detection, and is sent to the data analysis unit, to being examined
The data of survey are analyzed, and are then retransmited to the data processing unit, by data processing unit by the data measured with
Every 10-30 group data are that unit calculates its average value, and obtained average value is exported as testing result.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710704482.2A CN107576808B (en) | 2017-08-17 | 2017-08-17 | It is a kind of quickly to detect the remaining device and method of pesticide in fruit and vegetable food |
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| CN201710704482.2A CN107576808B (en) | 2017-08-17 | 2017-08-17 | It is a kind of quickly to detect the remaining device and method of pesticide in fruit and vegetable food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112857937A (en) * | 2021-01-25 | 2021-05-28 | 吉林省吉科软信息技术有限公司 | Automatic pesticide residue detection system based on food safety |
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| CN108168980B (en) * | 2018-02-11 | 2018-11-02 | 广东朗源生物科技有限公司 | A kind of pesticide and residues detection equipment |
| CN108802224B (en) * | 2018-06-14 | 2021-07-02 | 肖飞 | High accuracy traditional chinese medicine composition liquid chromatography analytical equipment |
| CN111443172A (en) * | 2020-04-17 | 2020-07-24 | 上海纽钛测控技术有限公司 | Distributed food detection method |
| CN111949623B (en) * | 2020-08-13 | 2022-02-01 | 广东省林业科学研究院 | Inspection data analysis and verification system |
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| CN107576808A (en) | 2018-01-12 |
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