CN107576676B - Three-dimensional molecular imaging system based on CT and optical fusion - Google Patents
Three-dimensional molecular imaging system based on CT and optical fusion Download PDFInfo
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Abstract
The invention provides a three-dimensional molecular imaging system based on CT and optical fusion, which comprises a data source transmitting module, a data acquisition module, a control system, a data transmission module, a data processing module and a bearing and sample rotating module, wherein the data acquisition module is used for acquiring data of a sample; the data source emission module is used for generating X rays and polarized light to irradiate an imaging sample; the data acquisition module is used for detecting and acquiring the X-ray dosage and polarized light intensity of the imaged sample; the control system and the data transmission module are connected with the data source transmitting module and the data acquisition module, and are used for controlling the movement of the data source transmitting module and the data acquisition module in the imaging process to obtain multimode projection data of different angles of an imaging sample and transmitting the multimode projection data to the data processing module; the data processing module is connected with the control system and the data transmission module and is used for processing the acquired multimode projection data of the imaging sample at different angles and reconstructing various tissue structure information of the imaging sample in a three-dimensional mode.
Description
Technical Field
The invention relates to the field of medical molecular imaging, in particular to a three-dimensional molecular imaging system based on CT and optical fusion.
Background
The medical molecular imaging technology can realize noninvasive, continuous, in-vivo and early visualization of the expression and activity of specific molecules affecting tumor behaviors and tumor response to drug treatment and physiological processes, and breaks through the limitation that the traditional imaging technology can only display anatomical structure changes caused by lesions.
With the continuous and deep research of medical molecular imaging, the conventional widely-used planar optical molecular imaging technology cannot quantitatively and three-dimensionally image an observed target, and cannot meet the requirements of biomedical research. Meanwhile, a single optical molecular imaging technology cannot provide comprehensive physiological and pathological information of organisms, and accurate diagnosis of serious diseases such as tumors and the like and accurate and effective evaluation of the curative effect of medicines are difficult to realize. Thus, some challenging problems related to multi-modal molecular imaging systems and methods have developed in recent years.
CT imaging is generally a tomographic image built by taking X-rays as a radioactive source, and data after detection scanning is collected to a control system for processing, reconstruction and display. CT imaging is stable, the technology is mature, but the contrast and resolution of soft tissue imaging of a transparent sample are low; optical projection tomography is widely used for imaging an in-vitro transparent sample of 1-10mm, but because of the strong light absorption characteristic of the sample in optical imaging, the imaging of high-density and density smooth tissues is limited, and especially the imaging effect on bones and muscles is not obvious.
It should be noted that the foregoing description of the background art is only for the purpose of providing a clear and complete description of the technical solution of the present invention and is presented for the convenience of understanding by those skilled in the art. The above-described solutions are not considered to be known to the person skilled in the art simply because they are set forth in the background of the invention section.
Disclosure of Invention
The invention provides a three-dimensional molecular imaging system based on CT and optical fusion, which aims to solve the problems that the existing imaging technology is limited in imaging of certain tissues and the imaging effect is not obvious.
In order to achieve the above purpose, the embodiment of the invention provides a three-dimensional molecular imaging system based on CT and optical fusion, which comprises a data source transmitting module, a data acquisition module, a control system, a data transmission module, a data processing module and a bearing and sample rotating module for bearing the data source transmitting module, the data acquisition module and an imaging sample; the data source emission module is used for generating X rays and polarized light to irradiate the imaging sample; the data acquisition module is used for detecting and acquiring the X-ray dosage and polarized light intensity passing through the imaging sample; the control system and the data transmission module are connected with the data source transmitting module and the data acquisition module and are used for controlling the movement of the data source transmitting module and the data acquisition module in the imaging process so as to obtain multimode projection data of different angles of the imaging sample and transmitting the multimode projection data to the data processing module; the data processing module is connected with the control system and the data transmission module and is used for processing the acquired multimode projection data of different angles of the imaging sample and reconstructing various tissue structure information of the imaging sample in a three-dimensional mode.
Further, in an embodiment, the data source emission module includes an X-ray emission sub-module, a white light source sub-module, an optical path adjustment sub-module, and a polarized light generation sub-module; the X-ray emission submodule is used for emitting X-rays to scan the imaging sample; the white light source sub-module is used for emitting white light to generate polarized light; the light path adjusting submodule is arranged on the white light path and used for enabling white light emitted by the white light source submodule to uniformly irradiate in all directions; the polarized light generating sub-module is used for setting and adjusting the angle of the polaroid so as to generate the polarized light to irradiate the imaging sample and adjust the polarized angle of the polarized light.
Further, in an embodiment, the data acquisition module includes an X-ray data acquisition sub-module, a polarized light data acquisition sub-module, and a polarized light filtering sub-module; the X-ray data acquisition sub-module is used for acquiring an X-ray signal after passing through the imaging sample and converting the X-ray signal into an electric signal; the polarized light filtering sub-module is arranged on a light path between the imaging sample and the polarized light data acquisition sub-module and is used for filtering out polarized light signals with a specific polarized angle; the polarized light data acquisition sub-module is used for acquiring the polarized light signals passing through the imaging sample and the polarized light filtering sub-module and converting the polarized light signals into electric signals.
Further, in an embodiment, the load and sample rotation module comprises a sample placement container and a rotating lever; the sample placing container is internally provided with an index matching liquid, the imaging sample is fixedly arranged under the rotary rod and is soaked in the index matching liquid, and the rotary rod is rotated to drive the imaging sample to rotate so as to obtain multimode projection data of different angles.
Further, in an embodiment, the control system and the data transmission module are configured to control emission switches and intensity control of the X-ray emission sub-module and the white light source sub-module, and control angle control of the polarizer in the polarized light generation sub-module.
Further, in an embodiment, the control system and data transmission module are configured to control angle control of the polarizer in the polarized light filtering sub-module.
Further, in an embodiment, the control system and the data transmission module are used for controlling the rotation speed and the rotation angle of the rotating rod.
Further, in an embodiment, the multi-mode projection data for different angles of the imaged sample comprises: multi-rotation angle X-ray imaging data and multi-rotation angle polarized light imaging data.
Further, in an embodiment, the data processing module includes: the data preprocessing sub-module is used for carrying out smooth noise reduction on the collected multi-rotation-angle X-ray imaging data and multi-rotation-angle polarized light imaging data; the white light imaging data acquisition sub-module is used for calculating and obtaining white light imaging data with multiple rotation angles according to the polarized light imaging data with multiple rotation angles; the polarization sensitive imaging data acquisition sub-module is used for calculating and obtaining the polarization sensitive imaging data with multiple rotation angles according to the polarization light imaging data with multiple rotation angles; an X-ray absorption coefficient distribution three-dimensional reconstruction sub-module, which is used for reconstructing an X-ray absorption coefficient distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle X-ray imaging data; the white light absorption coefficient distribution three-dimensional reconstruction sub-module is used for reconstructing a white light absorption coefficient distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle white light imaging data; the polarization sensitive tissue distribution three-dimensional reconstruction sub-module is used for reconstructing a polarization sensitive tissue distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle polarization sensitive imaging data; and the visualization and quantitative analysis sub-module is used for three-dimensionally drawing the X-ray absorption coefficient distribution image, the white light absorption coefficient distribution image and the polarization sensitive tissue distribution image of the imaging sample, and carrying out information fusion according to the spatial position to obtain the biological information of the imaging sample.
The three-dimensional molecular imaging system based on CT and optical fusion provided by the embodiment of the invention can be used for rapidly and simultaneously acquiring multi-mode images of X-ray tomography, transmission type optical projection tomography and polarization sensitive optical projection tomography of the inside of an isolated biological sample subjected to transparentization treatment, and can be used for rapidly obtaining information of X-ray absorption coefficient distribution, white light absorption coefficient distribution and polarization sensitive tissue distribution in the inside of the sample, wherein the information corresponds to bone tissue, low-density soft tissue and muscle tissue in the inside of the sample respectively, and different mode data can be fused directly according to a spatial position correlation. And finally, the imaging result is stereoscopically drawn by combining a visualization technology, so that a user can intuitively see the three-dimensional tomographic imaging result and perform analysis such as positioning, quantification and the like.
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In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained from these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic diagram of a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a data processing module 4 according to an embodiment of the present invention;
fig. 4 is a flowchart of a method for using a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Specific embodiments of the invention are disclosed in detail below with reference to a few representative embodiments of the invention, indicating the manner in which the principles of the invention may be employed. It should be understood that the embodiments of the invention are not limited in scope thereby. The embodiments of the invention include many variations, modifications and equivalents within the spirit and scope of the appended claims.
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments in combination with or instead of the features of the other embodiments.
It should be emphasized that the term "comprises/comprising" when used herein is taken to specify the presence of stated features, integers, steps or components but does not preclude the presence or addition of one or more other features, integers, steps or components.
The invention provides a three-dimensional molecular imaging system based on CT and optical fusion, which can rapidly and simultaneously acquire multi-mode images of X-ray tomography, transmission type optical projection tomography and polarization sensitive optical projection tomography of the inside of an isolated biological sample subjected to transparentization treatment, and can rapidly obtain X-ray absorption coefficient distribution, white light absorption coefficient distribution and polarization sensitive tissue distribution information of the inside of the sample, wherein the information corresponds to bone tissue, low-density soft tissue and muscle tissue of the inside of the sample respectively, and then the information is directly fused based on a spatial position relation. Finally, the imaging result is stereoscopically drawn by combining a visualization technology, so that a user can intuitively see the three-dimensional tomographic imaging result and perform analysis such as positioning, quantification and the like.
The three-dimensional molecular imaging system based on CT and optical fusion of the present invention is described in detail below with reference to the accompanying drawings.
Fig. 1 is a schematic structural diagram of a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention. Referring to fig. 1, the three-dimensional molecular imaging system based on CT and optical fusion includes four modules, respectively: the device comprises a data source transmitting module 1, a data acquisition module 2, a control system and data transmission module 3, a data processing module 4 and a bearing and sample rotating module 5 for bearing the data source transmitting module 1, the data acquisition module 2 and an imaging sample.
The data source emission module 1 is used for generating X rays and polarized light to irradiate the imaging sample; a data acquisition module 2 for detecting and acquiring the X-ray dose passing through the imaging sample and the polarized light intensity; the control system and data transmission module 3 is connected with the data source transmitting module 1 and the data acquisition module 2, and is used for controlling the movement of the data source transmitting module 1 and the data acquisition module 2 in the imaging process to obtain multimode projection data of different angles of the imaging sample, and transmitting the multimode projection data to the data processing module 4; the data processing module 4 is connected with the control system and the data transmission module 3 and is used for processing the acquired multimode projection data of different angles of the imaging sample and reconstructing various tissue structure information of the imaging sample in a three-dimensional mode.
FIG. 2 is a schematic structural diagram of a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention. As shown in fig. 2, the data source emitting module 1 includes an X-ray emitting sub-module 11, a white light source sub-module 12, an optical path adjusting sub-module 13, and a polarized light generating sub-module 14; the data acquisition module 2 comprises an X-ray data acquisition sub-module 21, a polarized light data acquisition sub-module 22 and a polarized light filtering sub-module 23; the carrying and sample rotation module 5 comprises a sample placement container 51 and a rotation lever 52; a control system and data transmission module 3, which is a computer system and associated connection components, not shown in fig. 2; the data processing module 4, which is a computer system and software, is not shown in fig. 2.
In this embodiment, the data source emitting module 1 is configured to emit X-rays and polarized light to irradiate an imaging sample, and includes:
an X-ray emitting sub-module 11 for emitting X-rays to scan the imaging sample;
a white light source sub-module 12 for emitting white light for generating polarized light;
the light path adjusting sub-module 13 is arranged on the white light path and is used for fixing ground glass, so that white light emitted by the white light source sub-module 12 is uniformly irradiated in all directions;
a polarized light generating sub-module 14 for setting and adjusting a polarizer angle to generate the polarized light to irradiate the imaging sample and adjust a polarization angle of the polarized light.
In this embodiment, the data acquisition module 2 is configured to detect an X-ray dose passing through a sample with an X-ray detector and detect polarized light intensity passing through the sample and an optical path with a camera, and includes:
an X-ray data acquisition sub-module 21 for acquiring an X-ray signal after passing through a sample using an X-ray detector and converting it into an electrical signal;
a polarized light data acquisition sub-module 22, configured to acquire a polarized light signal after passing through the sample and the optical path by using a high-sensitivity camera, and convert the polarized light signal into an electrical signal;
the polarized light filtering sub-module 23 is used for fixing and adjusting the angle of the polarizer so as to filter out the polarized light signal with a specific polarized angle.
In this embodiment, the carrying and sample rotation module 5 comprises a sample placement container 51 and a rotation lever 52. The sample placing container 51 is provided with an index matching liquid, the imaging sample is fixedly arranged under the rotary rod 52 and is soaked in the index matching liquid, and the rotary rod is rotated to drive the imaging sample to rotate, so that multimode projection data of different angles are obtained. Wherein the sample-holding container 51 may be a smooth transparent cube container in which an index matching fluid is held to attenuate refraction of light during transmission.
In addition, the carrying and sample rotating module 5 is further used for fixing the sub-modules such as an X-ray emitting sub-module 11, a white light source sub-module 12, a light path adjusting sub-module 13, a polarized light generating sub-module 14, an X-ray data collecting sub-module 21, a polarized light data collecting sub-module 22, a polarized light filtering sub-module 23 and the like included in the data source emitting module 1.
In this embodiment, the control system and the data transmission module 3 are used to control the normal and orderly operation among the modules of the system. For example, the module is used for realizing the emission switch control and the intensity control of an X-ray source and a white light source, the on-off control of an X-ray detector and a high-sensitivity camera, the data transmission to a computer, the angle control of a polaroid in a polarized light generating sub-module and a polarized light filtering sub-module and the rotation control of a sample. The module mainly comprises a control bus and a data transmission bus, and provides a visual operation interface for a user to conveniently and interactively operate the equipment according to requirements.
When sample imaging is performed, the sample is rotated 180 degrees or 360 degrees each time the sample is imaged, the angle of each step of sample rotation (such as 0.9 degrees, 1 degrees and the like of each step) can be adjusted, and the sample is stable after each step of sample rotation, so that specific X-ray imaging data and polarized light imaging data under the sample rotation angle can be obtained. The X-ray imaging data is signal data acquired by an X-ray detector after passing through a sample by irradiating the sample with X-rays.
The polarized light imaging data is signal data which is acquired by a high-sensitivity camera and passes through a sample and a light path, wherein the polarized light imaging data is obtained by rotating a polarized light sheet in the polarized light generating sub-module 14 by 180 degrees, and the polarized light sheet is averagely divided into a plurality of times (3 times or more) of rotation, and keeps stable after each rotation, and the polarized light sheet in the polarized light filtering sub-module 23 is respectively rotated to a polarized angle which is perpendicular to the polarized angle and parallel to the polarized angle.
In this embodiment, the data processing module 4 is configured to process each collected modal data, and reconstruct three-dimensionally various tissue structure information of a sample, as shown in fig. 3, which specifically includes:
a data preprocessing sub-module 41, configured to perform smooth noise reduction on the collected multi-rotation-angle X-ray imaging data and multi-rotation-angle polarized light imaging data;
the white light imaging data obtaining sub-module 42 is configured to calculate white light imaging data with multiple rotation angles according to the polarized light imaging data with multiple rotation angles. The method is specifically implemented by selecting, under each rotation angle of a sample, polarized light imaging data when the polarization angles of the polarizing plates in the polarizing light generating sub-module 14 are respectively parallel and perpendicular to the polarizing plates in the polarizing light filtering sub-module 23, and performing summation and then average processing to obtain white light imaging data.
The polarization sensitive imaging data obtaining sub-module 43 is configured to calculate multi-rotation angle polarization sensitive imaging data according to the multi-rotation angle polarized light imaging data. The specific implementation is that under each rotation angle of the sample, the polarized light imaging data when the polarization angle of the polarizer in the polarized light filtering sub-module 23 is perpendicular to the polarization angle of the polarizer in the polarized light generating sub-module 14 is selected, and the average processing is performed to obtain the polarization sensitive imaging data.
An X-ray absorption coefficient distribution three-dimensional reconstruction sub-module 44 for reconstructing an X-ray absorption coefficient distribution image of the imaging sample based on a filtered back-projection three-dimensional reconstruction algorithm and the multi-rotation angle X-ray imaging data;
a white light absorption coefficient distribution three-dimensional reconstruction sub-module 45, configured to reconstruct a white light absorption coefficient distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle white light imaging data;
a polarization sensitive tissue distribution three-dimensional reconstruction sub-module 46 for reconstructing a polarization sensitive tissue distribution image of the imaging sample based on a filtered back-projection three-dimensional reconstruction algorithm and the multi-rotation angle polarization sensitive imaging data;
the visualization and quantitative analysis sub-module 47 is configured to three-dimensionally draw an X-ray absorption coefficient distribution image, a white light absorption coefficient distribution image, and a polarization sensitive tissue distribution image of the imaging sample, and perform information fusion according to the spatial position, so as to obtain biological information of the imaging sample. The X-ray absorption coefficient distribution mainly reflects the distribution of bone tissues in the transparent sample, the white light absorption coefficient distribution mainly reflects the distribution of different low-density soft tissues in the transparent sample, and the polarization sensitive tissue distribution mainly reflects the distribution of fibrosis tissues (such as muscle tissues) in the transparent sample.
The following describes a method for using a three-dimensional molecular imaging system based on CT and optical fusion according to an embodiment of the present invention. As shown in fig. 4, the method includes:
step S1: the imaged sample is placed. The imaging sample is fixed under the rotating rod 52 of the carrying and sample rotating module 5 so as to be positioned at the center of the rotating rod 52 and immersed in the index matching liquid of the sample placing container 51. The other modules are stationary as the subsequent sample rotates.
Step S2: imaging data is acquired. And the control system and the data transmission module 3 send commands to enable the data source transmitting module 1 and the data acquisition module 2 to be started. So that when the sample is imaged, the sample rotates 180 degrees or 360 degrees each time the sample is imaged, the angle of each step of the sample rotation (such as 0.9 degrees, 1 degrees and the like of each step) is adjusted, and the sample is stable after each step of the sample rotation, so that specific X-ray imaging data and polarized light imaging data under the sample rotation angle are obtained. The X-ray imaging data is signal data acquired by an X-ray detector after passing through a sample by irradiating the sample with X-rays. The polarized light imaging data is signal data which is acquired by a high-sensitivity camera and passes through a sample and a light path, wherein the polarized light imaging data is obtained by rotating a polarized light sheet in the polarized light generating sub-module 14 by 180 degrees, and the polarized light sheet is averagely divided into a plurality of times (3 times or more) of rotation, and keeps stable after each rotation, and the polarized light sheet in the polarized light filtering sub-module 23 is respectively rotated to a polarized angle which is perpendicular to the polarized angle and parallel to the polarized angle.
Step S3: and (5) restoring the system state. And a control system and a data transmission module 3 send a command to enable the data source transmitting module 1 and the data acquisition module 2 to be closed, and an imaging sample is taken.
Step S4: and (5) data processing. Processing the acquired data by a data processing module:
firstly, preprocessing such as smooth noise reduction is carried out on collected multi-rotation-angle X-ray imaging data and polarized light imaging data.
White light imaging data and polarization sensitive imaging data are then processed from the polarized light imaging data. The white light imaging data is obtained by selecting the polarized light imaging data when the polarized angles of the polaroid in the polarized light filtering sub-module are respectively parallel and perpendicular to each other under each rotating angle of the sample and then carrying out summation and then average treatment; the polarization sensitive imaging data are obtained by selecting the polarization imaging data of the polarization plate in the polarization light generation sub-module under each rotation angle of the sample and carrying out average processing on the polarization imaging data when the polarization angle of the polarization plate in the polarization light filtering sub-module is vertical to the polarization angle.
And reconstructing an X-ray absorption coefficient distribution image, a white light absorption coefficient distribution image and a polarization sensitive tissue distribution image inside the transparent sample by using the multi-rotation-angle X-ray imaging data, the multi-rotation-angle white light imaging data and the multi-rotation-angle polarization sensitive imaging data based on a filtering back projection three-dimensional reconstruction algorithm.
And finally, fusing the image data of the three modes to obtain tissue detail information of the sample, and carrying out three-dimensional drawing on the X-ray absorption coefficient distribution, the white light absorption coefficient distribution and the polarization sensitive tissue distribution of the transparent imaging sample.
The three-dimensional molecular imaging system based on CT and optical fusion provided by the embodiment of the invention can be used for rapidly and simultaneously acquiring multi-mode images of X-ray tomography, transmission type optical projection tomography and polarization sensitive optical projection tomography of the inside of an isolated biological sample subjected to transparentization treatment, and can be used for rapidly obtaining information of X-ray absorption coefficient distribution, white light absorption coefficient distribution and polarization sensitive tissue distribution in the inside of the sample, wherein the information corresponds to bone tissue, low-density soft tissue and muscle tissue in the inside of the sample respectively, and different mode data can be fused directly according to a spatial position correlation. And finally, the imaging result is stereoscopically drawn by combining a visualization technology, so that a user can intuitively see the three-dimensional tomographic imaging result and perform analysis such as positioning, quantification and the like.
It will be appreciated by those skilled in the art that embodiments of the present invention may be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media (including, but not limited to, disk storage, CD-ROM, optical storage, and the like) having computer-usable program code embodied therein.
The present invention is described with reference to flowchart illustrations and/or block diagrams of methods, apparatus (systems) and computer program products according to embodiments of the invention. It will be understood that each flow and/or block of the flowchart illustrations and/or block diagrams, and combinations of flows and/or blocks in the flowchart illustrations and/or block diagrams, can be implemented by computer program instructions. These computer program instructions may be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, create means for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present invention; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present invention, the present description should not be construed as limiting the present invention in view of the above.
Claims (7)
1. The three-dimensional molecular imaging system based on CT and optical fusion is characterized by comprising a data source transmitting module, a data acquisition module, a control system, a data transmission module, a data processing module and a bearing and sample rotating module for bearing the data source transmitting module, the data acquisition module and an imaging sample;
the data source emission module is used for generating X rays and polarized light to irradiate the imaging sample;
the data acquisition module is used for detecting and acquiring the X-ray dosage and polarized light intensity passing through the imaging sample;
the control system and the data transmission module are connected with the data source transmitting module and the data acquisition module and are used for controlling the movement of the data source transmitting module and the data acquisition module in the imaging process so as to obtain multimode projection data of different angles of the imaging sample and transmitting the multimode projection data to the data processing module;
the data processing module is connected with the control system and the data transmission module and is used for processing the acquired multimode projection data of the imaging sample at different angles and reconstructing various tissue structure information of the imaging sample in a three-dimensional way;
the multi-mode projection data for different angles of the imaged sample comprises: multi-rotation angle X-ray imaging data and multi-rotation angle polarized light imaging data;
the data processing module comprises:
the data preprocessing sub-module is used for carrying out smooth noise reduction on the collected multi-rotation-angle X-ray imaging data and multi-rotation-angle polarized light imaging data;
the white light imaging data acquisition sub-module is used for calculating and obtaining white light imaging data with multiple rotation angles according to the polarized light imaging data with multiple rotation angles; the method specifically comprises the following steps:
under each rotation angle of a sample, selecting polarized light imaging data when the polarization angles of the polaroid in the polarized light generation sub-module are respectively parallel and perpendicular to the polarized light imaging data, and carrying out summation and then average treatment to obtain white light imaging data;
the polarization sensitive imaging data acquisition sub-module is used for calculating and obtaining the polarization sensitive imaging data with multiple rotation angles according to the polarization light imaging data with multiple rotation angles; the method specifically comprises the following steps:
under each rotation angle of the sample, selecting polarized light imaging data when the polarization angle of the polarizing plate in the polarized light generating sub-module is perpendicular to the polarization angle of the polarizing plate in the polarized light filtering sub-module, and carrying out average treatment to obtain polarization sensitive imaging data;
an X-ray absorption coefficient distribution three-dimensional reconstruction sub-module, which is used for reconstructing an X-ray absorption coefficient distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle X-ray imaging data;
the white light absorption coefficient distribution three-dimensional reconstruction sub-module is used for reconstructing a white light absorption coefficient distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle white light imaging data;
the polarization sensitive tissue distribution three-dimensional reconstruction sub-module is used for reconstructing a polarization sensitive tissue distribution image of the imaging sample based on a filtered back projection three-dimensional reconstruction algorithm and the multi-rotation angle polarization sensitive imaging data;
and the visualization and quantitative analysis sub-module is used for three-dimensionally drawing the X-ray absorption coefficient distribution image, the white light absorption coefficient distribution image and the polarization sensitive tissue distribution image of the imaging sample, and carrying out information fusion according to the spatial position to obtain the biological information of the imaging sample.
2. The three-dimensional molecular imaging system based on CT and optical fusion according to claim 1, wherein the data source emission module comprises an X-ray emission sub-module, a white light source sub-module, an optical path adjustment sub-module, and a polarized light generation sub-module;
the X-ray emission submodule is used for emitting X-rays to scan the imaging sample;
the white light source sub-module is used for emitting white light to generate polarized light;
the light path adjusting submodule is arranged on the white light path and used for enabling white light emitted by the white light source submodule to uniformly irradiate in all directions;
the polarized light generating sub-module is used for setting and adjusting the angle of the polaroid so as to generate the polarized light to irradiate the imaging sample and adjust the polarized angle of the polarized light.
3. The three-dimensional molecular imaging system based on CT and optical fusion of claim 1, wherein the data acquisition module comprises an X-ray data acquisition sub-module, a polarized light data acquisition sub-module, and a polarized light filtering sub-module;
the X-ray data acquisition sub-module is used for acquiring an X-ray signal after passing through the imaging sample and converting the X-ray signal into an electric signal;
the polarized light filtering sub-module is arranged on a light path between the imaging sample and the polarized light data acquisition sub-module and is used for filtering out polarized light signals with a specific polarized angle;
the polarized light data acquisition sub-module is used for acquiring the polarized light signals passing through the imaging sample and the polarized light filtering sub-module and converting the polarized light signals into electric signals.
4. The CT and optical fusion based three-dimensional molecular imaging system of claim 1, wherein the load-bearing and sample-rotating module comprises a sample-placement container and a rotating rod;
the sample placing container is internally provided with an index matching liquid, the imaging sample is fixedly arranged under the rotary rod and is soaked in the index matching liquid, and the rotary rod is rotated to drive the imaging sample to rotate so as to obtain multimode projection data of different angles.
5. The three-dimensional molecular imaging system based on CT and optical fusion according to claim 2, wherein the control system and data transmission module are configured to control the emission switch and intensity control of the X-ray emission sub-module and the white light source sub-module, and to control the angle control of the polarizer in the polarized light producing sub-module.
6. A three-dimensional molecular imaging system according to claim 3, wherein the control system and data transmission module are configured to control the angular control of the polarizer in the polarized light filter sub-module.
7. The three-dimensional molecular imaging system based on CT and optical fusion according to claim 4, wherein the control system and data transmission module are configured to control the rotational speed and rotational angle of the rotating rod.
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