CN107574186A - A kind of method added saccharomycete and make acidifying production natural pond system recover aerogenesis - Google Patents
A kind of method added saccharomycete and make acidifying production natural pond system recover aerogenesis Download PDFInfo
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- CN107574186A CN107574186A CN201710884716.6A CN201710884716A CN107574186A CN 107574186 A CN107574186 A CN 107574186A CN 201710884716 A CN201710884716 A CN 201710884716A CN 107574186 A CN107574186 A CN 107574186A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The present invention relates to technical field of biological fermentation, more particularly to a kind of method added saccharomycete and make acidifying production natural pond system recover aerogenesis, this method to specifically include following steps：In the acidifying production natural pond system, addition quality is its solid content（In terms of VS）0.8%~2.0%（W/W）Activated yeast, recovery aerogenesis is carried out under the conditions of 33 ~ 37 DEG C.Concentration of reduced sugar is not less than 15g/L in acidifying production natural pond system of the present invention, and Volatile fatty acid contents scope is 4g/L ~ 22g/L, and pH value is 4.50 ~ 5.80.This method can make the acidifying of stopping aerogenesis producing natural pond system recovery aerogenesis, and improve methane gas producing amount and the utilization ratio of biogas fermentative liquid；And this method step is simple, cost is cheap.
Description
Technical field
The present invention relates to technical field of biological fermentation, more particularly to one kind to make acidifying bionethanation system extensive by adding saccharomycete
The method for reproducing gas.
Background technology
Anaerobic digestion of kitchen wastes produces biogas as a kind of solid waste resource utilization approach, because it is simple, efficient
Process characteristic, cleaning, environmental protection technical concept and the outstanding contributions of energy-saving and emission-reduction are received much attention.By anaerobic fermentation,
40% ~ 50% organic principle can be converted into biogas in rubbish, and methane therein can be as the important replacement of traditional energy
And supplement, the situation of alleviation China's energy shortage；And remaining slaking residue can be used to produce organic fertilizer, house refuse is realized
Recycle, substantially reduce the solid waste total amount to environmental emission, to slow down urban environmental protection pressure and improve city life
State environment plays an important roll.
But because kitchen garbage content of organics is high, easily dropped in the hydrolysis acidification stage by the hydrolysis acidification bacterium in sludge
Solve as volatile fatty acid, such as acetic acid, propionic acid, butyric acid, and volatile fatty acid accumulation can be to methanogen to finite concentration
Acid is produced to suppress（Methanogen appropriate pH is 6.8 ~ 7.2）, cause anaerobic digester system can not normal operation, or even stop production
Gas, and then influence gas production and gas producing efficiency.
After bionethanation system is acidified, the method for common recovery system aerogenesis is to reduce inlet amount or even suspend charging
To control organic loading；If pH value can't be made to go up, nertralizer can be added to adjust pH value to 6.8.But in Practical Project, to
When the alkaline matters such as substantial amounts of lime, sodium hydroxide, potassium hydroxide are added in bionethanation system, some makes part not in time because of stirring
Alkalescence is too high, and some causes excessive addition because operation is improper, makes pH value is too high to cause methane backeria to inactivate；These alkalescence in addition
Inorganic substances addition can influence recycling of the biogas residue after anaerobic digestion as fertilizer.In consideration of it, Publication No.
CN103667101A patent application refer to recover bionethanation system acidification phenomenon using composite biological agent, but biology be present
The deficiency that preparation preparation procedure is complicated, cost is high, preservation condition is strict.
The content of the invention
In order to solve the above problems, it is adapted to the easy anaerobic fermentation system for being acidified substrate it is an object of the invention to provide a kind of,
And step is simple, cost is cheap, i.e., the acidifying bionethanation system of stopping aerogenesis being made to recover aerogenesis, improve methane gas producing amount again
Make the method for acidifying production natural pond system recovery aerogenesis with the addition saccharomycete of the utilization ratio of biogas fermentative liquid.
The technical scheme is that：A kind of side for making acidifying bionethanation system recover aerogenesis by adding activated yeast
Method, comprise the following steps that：
Step 1：The acidifying production natural pond system of aerogenesis to be restored is first got out, the concentration of reduced sugar in the system of natural pond is produced in detection acidifying；
Step 2：In the acidifying production natural pond system, addition quality is its solid content in terms of VS：0.8% ~ 2.0%W/W work
Change saccharomycete, anaerobic methane fermentation is carried out under the conditions of 33 DEG C ~ 37 DEG C, acidifying production natural pond system is recovered aerogenesis, and obtain biogas residue.
Further, whether concentration of reduced sugar is less than 15g/L in acidifying production natural pond system in the step 1, works as concentration of reduced sugar
During less than 15g/L, the saccharified liquid of glucose or substrate is added so that acid system reduced sugar initial concentration is not less than 15g/L.
Further, the Vfa Concentration scope in the step 1 in acidifying production natural pond system is 4g/L ~ 22g/L；
PH value range is 4.50 ~ 5.80.
Further, the addition of the activated yeast described in the step 2 can also be 0.8% ~ 1.5% (W/W)；Anaerobism
Fermentation temperature can also be 35 DEG C ~ 37 DEG C.
Further, the saccharomycete is saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC.No.1002 or fortune
Dynamic fermentation single cell bacterium（Zymomonas mobilis）The producing and ethanol bacterium such as CICC.No.10225, it is standby after actication of culture.
Further, described acidifying production natural pond system fermentation raw material is kitchen garbage, fruits and vegetables rubbish perishability debirs.
Further, acidifying production natural pond system acidificatoin time（Never aerogenesis starts to calculate）No more than 5d.
The invention has the advantages that：By adding activated yeast, activated yeast in the biogas fermentative liquid to acidifying
It can be survived under meta-acid environment, the carbon source by fighting for substrate digestion system flows to producing and ethanol rather than flow direction production acid, together
When because saccharomycete is a kind of protein in itself, its own, which is degraded, can increase the amino acid and ammonia-nitrogen content of system, neutralize acidifying
Biogas fermentative liquid afterwards, increase system basicity, and then recover production biogas, and ethanol can be converted into the second that methane backeria directly utilizes
Acid, improve the gas production and utilization ratio of biogas fermentative liquid.
（Three）Beneficial effect
The above-mentioned technical proposal of the present invention has the advantages that：It is of the present invention acidifying bionethanation system is recovered aerogenesis
Method, by adding activated yeast in the biogas fermentative liquid to acidifying, you can make the biogas fermentation of the stopping production biogas of acidifying
Liquid is gradually brought to neutrality, and then the biogas that resumes production, therefore improves the utilization ratio of biogas fermentative liquid；This method not only makes
It is acidified biogas fermentative liquid and recovers aerogenesis, more ethanol can be produced by activated yeast, and ethanol is more easy to be changed into acetic acid, is produced
Methane backeria utilizes, and further improves the gas production of biogas, therefore this method achieves this unexpected technique effect；The party
Method need not prepare the biological agent of high cost, it is not necessary to which complicated matches somebody with somebody bacterium process, you can and acidifying bionethanation system is recovered aerogenesis,
Not only step is few, and method is easy, and cost is cheap, is easy to engineering construction.
Brief description of the drawings
Fig. 1 is each embodiment and comparative example cumulative gas curve synoptic diagram using the method for the present invention.
Fig. 2 is each embodiment and comparative example pH value change curve schematic diagram using the method for the present invention.
Fig. 3 is each embodiment and comparative example basicity change curve schematic diagram using the method for the present invention.
Embodiment
Embodiments of the present invention are described in further detail with reference to embodiment.Following examples are used to illustrate this
Invention, but can not be used for limiting the scope of the present invention.
Acidifying biogas fermentative liquid used in of the invention is for kitchen garbage with anaerobic sludge digestion gained, it is necessary to which explanation, is somebody's turn to do
Acidifying biogas fermentative liquid is merely illustrative and description for the sake of provide, rather than say that the method for the invention is only applicable to the acidifying natural pond
Gas zymotic fluid.
It is described acidifying biogas fermentative liquid production method be：Kitchen garbage 2000ml, it is inoculated with long-term acclimation sludge 2000ml
（Inoculation acclimation sludge is selected from the secondary sedimentation tank of sewage treatment work mud extraction of Xiao Jia rivers）, nitrogen is filled with after being sufficiently mixed to obtain anaerobism ring
Border, and anaerobic fermentation is carried out at 35 DEG C, bionethanation system pH value is down to 5.85 after 3 days, and 7d pH value is 5.15, and reduced sugar is dense
It is required acidifying biogas fermentative liquid to spend for 4.23g/L, 7d biogas slurries.
A kind of method added saccharomycete and make acidifying production natural pond system recover aerogenesis of the present invention, this method specifically include following step
Suddenly：
Step 1：The acidifying production natural pond system of aerogenesis to be restored is first got out, the concentration of reduced sugar in the system of natural pond is produced in detection acidifying；
Step 2：In the acidifying production natural pond system, addition quality is its solid content in terms of VS：0.8% ~ 2.0%W/W work
Change saccharomycete, anaerobic methane fermentation is carried out under the conditions of 33 DEG C ~ 37 DEG C, acidifying production natural pond system is recovered aerogenesis, and obtain biogas residue.
Whether concentration of reduced sugar is less than 15g/L in acidifying production natural pond system in the step 1, when concentration of reduced sugar is less than 15g/
During L, the saccharified liquid of glucose or substrate is added so that acid system reduced sugar initial concentration is not less than 15g/L.
Vfa Concentration scope in the step 1 in acidifying production natural pond system is 4g/L ~ 22g/L；PH value range
For 4.50 ~ 5.80.
The addition of activated yeast described in the step 2 can also be 0.8% ~ 1.5% (W/W)；Anaerobic fermentation temperature
Degree can also be 35 DEG C ~ 37 DEG C.
The saccharomycete is saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC.No.1002 or motion fermentation
Monad（Zymomonas mobilis）The producing and ethanol bacterium such as CICC.No.10225, it is standby after actication of culture.
Described acidifying production natural pond system fermentation raw material is kitchen garbage, fruits and vegetables rubbish perishability debirs.
Acidifying production natural pond system acidificatoin time（Never aerogenesis starts to calculate）No more than 5d.
Embodiment 1
A kind of method for making acidifying produce natural pond system recovery aerogenesis by adding saccharomycete is present embodiments provided, i.e.,：Take acidifying natural pond
Gas zymotic fluid 400ml, to ensure that activation ethanol bacterium mushrooms out, add kitchen garbage saccharified liquid（Do not centrifuge）100ml, now also
Raw sugar concentration is 16.2g/L, and solid content 43.2g, activated yeast addition is acidifying biogas fermentative liquid solid content
Content（In terms of VS）0.8%, nitrogen is filled with after well mixed and ensures anaerobic environment, anaerobic methane fermentation is carried out at 35 DEG C.
Embodiment 2
A kind of method for making acidifying produce natural pond system recovery aerogenesis by adding saccharomycete is present embodiments provided, with the base of embodiment 1
This is identical, only difference is that：Activated yeast quality addition is acidifying biogas fermentative liquid solid content（In terms of VS）'s
1.5%。
Embodiment 3
A kind of method for making acidifying produce natural pond system recovery aerogenesis by adding saccharomycete is present embodiments provided, with the base of embodiment 1
This is identical, only difference is that：Activated yeast addition is acidifying biogas fermentative liquid solid content（In terms of VS）2.0%.
By the methods described of above-described embodiment 1 ~ 3, after acidifying biogas fermentative liquid recovers methane phase, accumulative methane production is respectively
1971ml, 2556ml and 2214ml（Referring to Fig. 1）, during pH value change referring to Fig. 2.
Analyzed from Fig. 1 and Fig. 2, under the activated yeast addition of above-mentioned three kinds of embodiments, basicity increase（Figure
3）, pH value is gradually brought to neutrality, and this provides suitable living environment to methanogen, the breeding of methanogen raised growth,
Metabolic activity is carried out using the organic acid in biogas slurry, produces substantial amounts of methane so that acidifying production natural pond system is recovered, but after arriving
Phase stops aerogenesis because the substrate of bionethanation system is depleted system, now according to mode same as the previously described embodiments, according to reality
Border needs, circulation supplement substrate, you can make the biogas fermentative liquid continue aerogenesis.It should be noted that circulation addition substrate
Method and its similar approach, equally within protection scope of the present invention.
Comparative example 1
Acidifying digestive juice 400ml same as the previously described embodiments is taken, with finite concentration alkali lye（The alkali such as sodium hydroxide, potassium hydroxide
Property material）Adjust and carry out recovering aerogenesis experiment under pH value to 6.0, with embodiment the same terms of acidification system.
Comparative example 2
Acidifying digestive juice 400ml same as the previously described embodiments is taken, does not add any material, is entered under the same conditions with embodiment
Row recovers aerogenesis experiment.
The aerogenesis result of comparative example is shown in Fig. 1, during pH value change see Fig. 2.Fig. 3 is shown in basicity change.
Interpretation of result shows, each embodiment methods described and comparative example 1 methods described（That is tradition plus alkali lye recovers aerogenesis side
Method）So that acidifying production natural pond system recovers aerogenesis, each embodiment recovery gas production is higher than the recovery gas production of comparative example 1, says
Bright embodiment methods described can not only be recovered rapidly to be acidified bionethanation system aerogenesis, can also improve biogas output.Comparative example 2
As a result there occurs irreversible acidifying for explanation acidifying production natural pond system, it is impossible to voluntarily recovers aerogenesis.
Embodiments of the invention provide for the sake of example and description, and are not exhaustively or by this to send out
It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing
Select and describe embodiment and be to more preferably illustrate the principle and practical application of the present invention, and make one of ordinary skill in the art
It will be appreciated that the present invention is so as to designing the various embodiments with various modifications suitable for special-purpose.
Claims (7)
1. it is a kind of add saccharomycete make acidifying production natural pond system recover aerogenesis method, it is characterised in that this method specifically include with
Lower step：
Step 1：The acidifying production natural pond system of aerogenesis to be restored is first got out, the concentration of reduced sugar in the system of natural pond is produced in detection acidifying；
Step 2：In the acidifying production natural pond system, addition quality is its solid content in terms of VS：0.8% ~ 2.0%W/W work
Change saccharomycete, anaerobic methane fermentation is carried out under the conditions of 33 DEG C ~ 37 DEG C, acidifying production natural pond system is recovered aerogenesis, and obtain biogas residue.
2. according to the method for claim 1, it is characterised in that concentration of reduced sugar in acidifying production natural pond system in the step 1
Whether it is less than 15g/L, when concentration of reduced sugar is less than 15g/L, adds the saccharified liquid of glucose or substrate so that acid system is also
Raw sugar initial concentration is not less than 15g/L.
3. according to the method for claim 1, it is characterised in that the volatility fat in the step 1 in acidifying production natural pond system
Fat acid concentration range is 4g/L ~ 22g/L；PH value range is 4.50 ~ 5.80.
4. according to the method for claim 1, it is characterised in that the addition of the activated yeast described in the step 2
Can also be 0.8% ~ 1.5% (W/W)；Anaerobic fermentation temperature can also be 35 DEG C ~ 37 DEG C.
5. according to the method for claim 1, it is characterised in that the saccharomycete is saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC.No.1002 or zymomonas mobilis（Zymomonas mobilis）CICC.No.10225 etc. produces second
Alcohol bacterium, it is standby after actication of culture.
6. according to the method described in right 1, it is characterised in that described acidifying production natural pond system fermentation raw material is kitchen garbage, fruit
Vegetable rubbish perishability debirs.
7. according to the methods described of right 1, it is characterised in that acidifying production natural pond system acidificatoin time（Never aerogenesis starts to calculate）Do not surpass
Cross 5d.
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| CN201710884716.6A CN107574186B (en) | 2017-09-26 | 2017-09-26 | Method for recovering gas production of acidification methane production system by adding saccharomycetes |
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| CN103667101A (en) * | 2013-09-26 | 2014-03-26 | 中国科学院成都生物研究所 | Compound biological agent for enabling acidified marsh gas fermentation system to restore gas production and preparation method thereof |
| CN104561222A (en) * | 2014-03-24 | 2015-04-29 | 浙江大学 | Kitchen waste and sludge hydrogen-producing acidification pretreatment method capable of increasing methane production rate |
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| JP2016185123A (en) * | 2015-03-27 | 2016-10-27 | 学校法人常翔学園 | Method for producing methane gas from waste |
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