CN107569718A - A kind of preparation method of 3D printing PLA and bacteria cellulose compound support frame material - Google Patents
A kind of preparation method of 3D printing PLA and bacteria cellulose compound support frame material Download PDFInfo
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- CN107569718A CN107569718A CN201710762907.5A CN201710762907A CN107569718A CN 107569718 A CN107569718 A CN 107569718A CN 201710762907 A CN201710762907 A CN 201710762907A CN 107569718 A CN107569718 A CN 107569718A
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Abstract
A kind of preparation method of 3D printing PLA and bacteria cellulose compound support frame material, belongs to tissue engineering technique field.Methods described concretely comprises the following steps：1st, strain fermentation prepares bacteria cellulose, is washed successively and alkali cleaning；2nd, at ambient temperature, bacterial cellulose suspension is prepared, suction filtration is then depressurized and obtains bacterial cellulose film, freeze-drying obtains bacterial cellulose three-dimensional porous support；3rd, 3D printing is carried out, obtains 3D printing PLA and bacteria cellulose compound support frame material.It is an advantage of the invention that：Preparation method of the present invention is simple, and the operation cycle is short, and can be by controlling technological parameter accuracy controlling support top layer pore size and shape during 3D printing.The 3D printing PLA of preparation and the compound support frame material of bacteria cellulose both have good mechanical performance, there is higher porosity and excellent biocompatibility again, Adhering capacity of the cell on timbering material surface can also be improved, therefore can be used for field of tissue engineering technology.
Description
Technical field
The invention belongs to tissue engineering technique field, and in particular to a kind of 3D printing PLA and bacteria cellulose composite support
The preparation method of frame material.
Background technology
Organizational project, it is that application project, biology and materialogy are combined by one kind also referred to as " regenerative medicine engineering "
Principle and technology.The mode generally realized is to build the three-dimensional porous biomimetic features similar with extracellular matrix, mould in vitro
Intend the growing environment of cell, be the attachment of cell and the suitable environment of offer is provided, meanwhile, the three-dimensional porous structure mutually run through
Nutrient can be provided for the growth of cell and propagation, and good passage and space are provided for metabolite and gas exchanges.
Preferable tissue engineering material needs to have suitable shape and volume to meet actual application demand, also simultaneously
Should have higher porosity, excellent biocompatibility to provide good environment for the growth of cell, meanwhile, should also possess excellent
Different mechanical performance, degradability are to ensure the application of clinic.Bacteria cellulose（Bacterial Cellulose, BC）As normal
Medical macromolecular materials, possess the excellent properties of the above, be one of preferable tissue engineering material.
3D printing（3D Printing）Technology is a kind of new rapid shaping technique, and the technology is with Computer Design
Build model based on, with plastics or powdery metal etc. can jointing material, demand is obtained by way of successively printing
Complex three-dimensional structure.It just can obtain three-dimensional structure, so as to substantially reduce without machining and mould from computer model
The lead time of product, so that cost reduces while productivity ratio is largely increased, therefore in bioengineering and medical domain
With very big development potentiality.
The preparation method of existing frequently-used timbering material has：Freeze-drying and solution casting method.Two methods operation is numerous
Trivial, production cycle length, the shape and thickness of timbering material depend on mould, and this causes its application to be very restricted.
The content of the invention
The invention aims to the problems such as cumbersome, the mould dependence for solving to presently, there are, there is provided a kind of 3D
The preparation method of PLA and bacteria cellulose compound support frame material is printed, methods described utilizes 3D printing technique, can prepare one
The porous composite support of tissue engineering material of kind.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of preparation method of 3D printing PLA and bacteria cellulose compound support frame material, methods described comprise the following steps that：
Step 1：Choose bacterial strain and bacteria cellulose is prepared by static culture method fermentation, obtained bacteria cellulose is entered successively
Water-filling is washed and alkali cleaning；
Step 2：At ambient temperature, the bacteria cellulose obtained using step 1 prepares bacterial cellulose suspension, Ran Houjing
Cross decompression suction filtration and obtain bacterial cellulose film, freeze-drying obtains bacterial cellulose three-dimensional porous support；
Step 3：3D printing is carried out to the bacterial cellulose three-dimensional porous support of step 2, obtains having the 3D of high porosity to beat
Print PLA and bacteria cellulose compound support frame material.
It is of the invention to be relative to the beneficial effect of prior art：Support prepared by the present invention can be used as tissue engineering bracket,
Applied to tissue repair and rebuild field.Preparation method of the present invention is simple, and the operation cycle is short, and can be by controlling 3D printing process
In technological parameter accuracy controlling support top layer pore size and shape.The 3D printing PLA of preparation and answering for bacteria cellulose
Closing timbering material both has good mechanical performance, has higher porosity again（60~95%）And excellent biocompatibility,
Adhering capacity of the cell on timbering material surface can also be improved, therefore can be used for field of tissue engineering technology.
Embodiment
Technical scheme is further described with reference to embodiment, but is not limited thereto, it is every right
Technical solution of the present invention is modified or equivalent substitution, without departing from the spirit and scope of technical solution of the present invention, all should contain
Cover in protection scope of the present invention.
Embodiment one：What present embodiment was recorded is a kind of 3D printing PLA and bacteria cellulose compound rest
The preparation method of material, methods described comprise the following steps that：
Step 1：Choose bacterial strain and bacteria cellulose is prepared by static culture method fermentation, obtained bacteria cellulose is entered successively
Water-filling is washed and alkali cleaning；
Step 2：At ambient temperature, the bacteria cellulose obtained using step 1 prepares bacterial cellulose suspension, Ran Houjing
Cross decompression suction filtration and obtain bacterial cellulose film, freeze-drying obtains bacterial cellulose three-dimensional porous support；
Step 3：3D printing is carried out to the bacterial cellulose three-dimensional porous support of step 2, obtains the 3D with more high porosity
Print PLA and bacteria cellulose compound support frame material.
Embodiment two：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 1, the bacterial strain is rhizobium（Rhizobium）, acetic acid Pseudomonas
（Acetobacter）, Sarcina（Sarcina）, Agrobacterium（Agrobacterium）, azotobacter
（Azotobacter）, achromobacter（Achromobacter）Or Alcaligenes（Alcaligenes）In one or more.
Embodiment three：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 1, described method for washing is to use deionized water rinsing 3 ~ 5 times or multiple with deionized water
Soak 10 ~ 30 min；Described alkali washing method is placed in bacteria cellulose in the NaOH solution that mass percent is 2 ~ 9 %,
2 ~ 7 h are heated at a temperature of 30 ~ 100 DEG C.
Embodiment four：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 2, the preparation method of described bacterial cellulose suspension is, at ambient temperature, first
Bacteria cellulose after purification is cut into small pieces, is placed in mixer, then adds suitable quantity of water so that the weight of bacteria cellulose
Percentage is 2.6%, 0.66% or 0.33%, and 10 ~ 30 min of stirring obtain uniform bacterial cellulose suspension.
Embodiment five：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 2, it be 5 ~ 10 min that described decompression, which filters the time, and decompressed pressure is -0.08 ~ -0.1
MPa。
Embodiment six：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 2, described freeze-drying temperature is -20 DEG C ~ -60 DEG C, and drying time is 12 ~ 48 h.
Embodiment seven：A kind of 3D printing PLA and bacteria cellulose composite support described in embodiment one
The preparation method of frame material, in step 3, described 3D printing is：Porous film material needed for establishing in a computer is three-dimensional porous
The 3D models of support, then 3D printing is added in 3D printer barrel with PLA powder, will by way of successively printing
PLA is printed upon on the bacteria cellulose membrane material being placed on three-dimensional wave moving platform, obtains 3D printing PLA and bacterial fibers
The compound support frame material of element.
Embodiment 1：
Using static culture method, bacteria cellulose is obtained by rhizobium fermented and cultured, with deionized water rinsing 3 ~ 5 times, removed
The culture medium and impurity on bacteria cellulose surface are adhered to, is soaked in afterwards in the NaOH aqueous solution that percentage by weight is 1%,
2 h are heated at a temperature of 100 DEG C, it is neutral that taking-up, which continues to be rinsed repeatedly with deionized water to pH value,.
The bacteria cellulose cleaned is mechanically cut into small block-shaped, is placed in mixer and stirs into bacteria cellulose
Homogeneous slurry.Take 10 ~ 30 g homogeneous slurries to filter 5 ~ 10 min and obtain bacteria cellulose film.Under the conditions of -20 DEG C ~ -60 DEG C,
3D printing is carried out after freeze-drying.
The concentration of slurry is that the percentage by weight of bacteria cellulose is 2.6 %.
The concrete operation step of the 3D printing quick molding method is：（1）Modeling：It is porous needed for establishing in a computer
The 3D models of membrane material support, above-mentioned 3D models are regular borehole structure, a diameter of 1 mm of circular hole, are spaced between circular hole
0.5 mm, while the thin slice that model decomposition is 5 layers 20 μm, final PLA thickness are amounted into 1 mm；（2）Charging：By 3D printing
Added with PLA powder in 3D printer barrel；（3）Printing：PLA is printed upon by way of successively printing and is placed in three
On bacteria cellulose membrane material on dimension fluctuation platform.
Embodiment 2：
Using static culture method, bacteria cellulose is obtained by acetobacter xylinum category fermented and cultured, with deionized water rinsing 3 ~ 5 times, removed
Remove to be adhered to the culture medium and impurity on bacteria cellulose surface, be soaked in afterwards in the NaOH aqueous solution that percentage by weight is 3%,
3h is heated at a temperature of 80 DEG C, it is neutral that taking-up, which continues to be rinsed repeatedly with deionized water to pH value,.
By the bacteria cellulose cleaned be mechanically cut into it is small it is block-shaped be placed in mixer, and add appropriate deionization
Water, stir into the homogeneous slurry of bacteria cellulose.Take 10 ~ 30 g homogeneous slurries to filter 5 ~ 10 min and obtain bacteria cellulose film.
Under the conditions of -20 DEG C ~ -60 DEG C, 3D printing is carried out after freeze-drying.
The percentage by weight of the slurries bacteria cellulose is 0.66 %.
The concrete operation step of the 3D printing quick molding method is：（1）Modeling：It is porous needed for establishing in a computer
The 3D models of membrane material support, above-mentioned 3D models are regular borehole structure, a diameter of 0.5mm of circular hole, are spaced between circular hole
0.5 mm, while the thin slice that model decomposition is 5 layers 20 μm, final PLA thickness are amounted to 100 μm；（2）Charging：3D is beaten
Print is added in 3D printer barrel with PLA powder；（3）Printing：PLA is printed upon by way of successively printing and is placed in
On bacteria cellulose membrane material on three-dimensional wave moving platform.
Embodiment 3：
Using static culture method, bacteria cellulose is obtained by Sarcina fermented and cultured, with deionized water rinsing 3 ~ 5 times, removed
Remove to be adhered to the culture medium and impurity on bacteria cellulose surface, be soaked in afterwards in the NaOH aqueous solution that percentage by weight is 5%,
4 h are heated at a temperature of 60 DEG C, it is neutrality to continue to spend ionized water and rinse repeatedly to pH value after taking-up.
By the bacteria cellulose cleaned be mechanically cut into it is small it is block-shaped be placed in mixer, and add appropriate deionization
Water, stir into the homogeneous slurry of bacteria cellulose.Take 10 ~ 30 g homogeneous slurries to filter 5 ~ 10 min and obtain bacteria cellulose film.
Under the conditions of -20 DEG C ~ -60 DEG C, 3D printing is carried out after freeze-drying.
The percentage by weight of the slurries bacteria cellulose is 0.33 %.
The concrete operation step of the 3D printing quick molding method is：（1）Modeling：It is porous needed for establishing in a computer
The 3D models of membrane material support, above-mentioned 3D models are regular borehole structure, a diameter of 0.8mm of circular hole, are spaced between circular hole
0.8 mm, while the thin slice that model decomposition is 5 layers 20 μm, final PLA thickness are amounted into 1 mm；（2）Charging：By 3D printing
Added with PLA powder in 3D printer barrel；（3）Printing：PLA is printed upon by way of successively printing and is placed in three
On bacteria cellulose membrane material on dimension fluctuation platform.
Claims (7)
- A kind of 1. preparation method of 3D printing PLA and bacteria cellulose compound support frame material, it is characterised in that：Methods described Comprise the following steps that：Step 1：Choose bacterial strain and bacteria cellulose is prepared by static culture method fermentation, obtained bacteria cellulose is entered successively Water-filling is washed and alkali cleaning；Step 2：At ambient temperature, the bacteria cellulose obtained using step 1 prepares bacterial cellulose suspension, Ran Houjing Cross decompression suction filtration and obtain bacterial cellulose film, freeze-drying obtains bacterial cellulose three-dimensional porous support；Step 3：3D printing is carried out to the bacterial cellulose three-dimensional porous support of step 2, obtains having the 3D of high porosity to beat Print PLA and bacteria cellulose compound support frame material.
- 2. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 1, the bacterial strain is rhizobium, acetic acid Pseudomonas, Sarcina, Agrobacterium, nitrogen-fixing bacteria One or more in category, achromobacter or Alcaligenes.
- 3. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 1, described method for washing is repeatedly to soak 10 with deionized water rinsing 3 ~ 5 times or with deionized water ~30 min；Described alkali washing method is placed in bacteria cellulose in the NaOH solution that mass percent is 2 ~ 9 %, 30 ~ 2 ~ 7 h are heated at a temperature of 100 DEG C.
- 4. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 2, the preparation method of described bacterial cellulose suspension is at ambient temperature, will first to purify Bacteria cellulose afterwards is cut into small pieces, and is placed in mixer, then adds suitable quantity of water so that the percentage by weight of bacteria cellulose For 2.6%, 0.66% or 0.33%, 10 ~ 30 min of stirring obtain uniform bacterial cellulose suspension.
- 5. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 2, the described decompression suction filtration time is 5 ~ 10 min, and decompressed pressure is -0.08 ~ -0.1 MPa.
- 6. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 2, described freeze-drying temperature is -20 DEG C ~ -60 DEG C, and drying time is 12 ~ 48 h.
- 7. the preparation method of a kind of 3D printing PLA according to claim 1 and bacteria cellulose compound support frame material, It is characterized in that：In step 3, described 3D printing is：Porous film material three-dimensional porous rack needed for establishing in a computer 3D models, then 3D printing is added in 3D printer barrel with PLA powder, by PLA by way of successively printing It is printed upon on the bacteria cellulose membrane material being placed on three-dimensional wave moving platform, obtains answering for 3D printing PLA and bacteria cellulose Close timbering material.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108327241A (en) * | 2018-01-28 | 2018-07-27 | 浙江大学 | A kind of manufacturing method of controllable antibacterial trachea bracket |
| CN110201235A (en) * | 2019-07-15 | 2019-09-06 | 湖南大学 | A kind of novel tissue reparation pad pasting |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1870115A1 (en) * | 2006-06-22 | 2007-12-26 | Regentis Biomaterials Ltd. | Matrix composed of a naturally-occurring protein backbone cross linked by a synthetic polymer and methods of generating and using same |
| CN103387688A (en) * | 2013-08-19 | 2013-11-13 | 南京林业大学 | Preparation method of cellulose nano-fiber/polylactic acid composite membrane |
| CN103691005A (en) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | Micro-nanofiber tissue engineering scaffold and preparation method thereof |
| CN103751852A (en) * | 2014-01-24 | 2014-04-30 | 天津理工大学 | Preparation method of three-dimensional artificial random porous structure tissue engineering scaffold |
| CN104072809A (en) * | 2014-06-20 | 2014-10-01 | 南京林业大学 | Preparation method of graphene oxide/bacterial cellulose antibacterial compound material |
| CN104225669A (en) * | 2014-08-25 | 2014-12-24 | 华南理工大学 | Bioactive bacterial cellulose-zein composite film and preparation method thereof |
| CN104383585A (en) * | 2014-11-29 | 2015-03-04 | 山东省立医院 | Three-dimensional nanocomposite adhesive bandage as well as preparation method and use method thereof |
| US20180298370A1 (en) * | 2015-04-27 | 2018-10-18 | Julius-Maximilians-Universität Würzburg | Modified bacterial nanocellulose and its uses in chip cards and medicine |
-
2017
- 2017-08-30 CN CN201710762907.5A patent/CN107569718A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1870115A1 (en) * | 2006-06-22 | 2007-12-26 | Regentis Biomaterials Ltd. | Matrix composed of a naturally-occurring protein backbone cross linked by a synthetic polymer and methods of generating and using same |
| CN103387688A (en) * | 2013-08-19 | 2013-11-13 | 南京林业大学 | Preparation method of cellulose nano-fiber/polylactic acid composite membrane |
| CN103691005A (en) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | Micro-nanofiber tissue engineering scaffold and preparation method thereof |
| CN103751852A (en) * | 2014-01-24 | 2014-04-30 | 天津理工大学 | Preparation method of three-dimensional artificial random porous structure tissue engineering scaffold |
| CN104072809A (en) * | 2014-06-20 | 2014-10-01 | 南京林业大学 | Preparation method of graphene oxide/bacterial cellulose antibacterial compound material |
| CN104225669A (en) * | 2014-08-25 | 2014-12-24 | 华南理工大学 | Bioactive bacterial cellulose-zein composite film and preparation method thereof |
| CN104383585A (en) * | 2014-11-29 | 2015-03-04 | 山东省立医院 | Three-dimensional nanocomposite adhesive bandage as well as preparation method and use method thereof |
| US20180298370A1 (en) * | 2015-04-27 | 2018-10-18 | Julius-Maximilians-Universität Würzburg | Modified bacterial nanocellulose and its uses in chip cards and medicine |
Non-Patent Citations (2)
| Title |
|---|
| DE SOUZA等: "Vaccum dried membranes of Poly (l-lactic acid) and bacterial cellulose for biomedical application", 《BMC PROCEEDINGS》 * |
| 王运赣，王宣: "《三维打印技术》", 31 July 2013 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108327241A (en) * | 2018-01-28 | 2018-07-27 | 浙江大学 | A kind of manufacturing method of controllable antibacterial trachea bracket |
| CN108327241B (en) * | 2018-01-28 | 2019-09-20 | 浙江大学 | A kind of manufacturing method of controllable antibacterial trachea bracket |
| CN110201235A (en) * | 2019-07-15 | 2019-09-06 | 湖南大学 | A kind of novel tissue reparation pad pasting |
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Inventor after: Wu Yadong Inventor after: Wang Yunfeng Inventor after: Huang Yudong Inventor after: He Jinmei Inventor after: Wang Fang Inventor after: Cheng Feng Inventor after: Wei Xinjing Inventor before: Wu Yadong Inventor before: Wang Yunfeng Inventor before: He Jinmei Inventor before: Wang Fang Inventor before: Cheng Feng Inventor before: Wei Xinjing |
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