CN107561150A - The detection method of drug abuse - Google Patents
The detection method of drug abuse Download PDFInfo
- Publication number
- CN107561150A CN107561150A CN201710533241.6A CN201710533241A CN107561150A CN 107561150 A CN107561150 A CN 107561150A CN 201710533241 A CN201710533241 A CN 201710533241A CN 107561150 A CN107561150 A CN 107561150A
- Authority
- CN
- China
- Prior art keywords
- detection method
- drug abuse
- drug
- drugs
- saliva
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a kind of detection method of drug abuse, the detection method of the drug abuse comprises the following steps：(A1) according to influence of the drugs to human body, the characteristic ion peak of Mass Spectrometer Method corresponding with drugs is obtained；(A2) saliva of drug addict is accused of in collection；(A3) extractant is added in saliva, extracts and stand；(A4) Aspirate supernatant；(A5) supernatant is detected using mass spectrograph, obtains the intensity at the characteristic ion peak；(A6) it is accused of whether drug addict takes drugs according to the intensity, threshold decision.The present invention has the advantages that cost is low, is pre-processed without complexity, high sensitivity.
Description
Technical field
The present invention relates to detection of taking drugs, the method for more particularly to detecting whether to take drugs using saliva.
Background technology
The method that tradition is used for detecting drugs is combined gas chromatography mass spectrometry, and Main Analysis sample is blood or urine, is passed through
Chromatographic isolation is first carried out to blood or urine, enters back into its composition of mass spectral analysis and structure.At present, new detection method is saliva
Liquid combined detection reagent method, by collaurum monoclonal antibodies, antibody mediated immunity Competition principle detect saliva in drugs into
Point.And illicit drugs inspection all having time windows in blood, urine, saliva, a few houres or several days are commonly, saliva only has 12-24 small
When.
Both analysis methods mainly have following deficiency：Combined gas chromatography mass spectrometry needs more complicated sample pretreatment, operation
Cumbersome, analysis time length, sample easily misses detection time window and obtains false negative result；Saliva combined detection reagent method detects
Project is few, and single detection is expensive, and easy flase drop obtains false positive or false negative result；This both of which can only judge to inhale
Whether malicious person relapses, it is impossible to judges that drug addict quits drug abuse the length of time, so as to infer time that drug addict consumes illegal drugs the last time.
The content of the invention
To solve the deficiency in above-mentioned prior art, the invention provides a kind of cost it is low, without complicated pretreatment,
The detection method of the drug abuse of high sensitivity.
The purpose of the present invention is achieved through the following technical solutions：
The detection method of drug abuse, the detection method of the drug abuse comprise the following steps：
(A1) according to influence of the drugs to human body, the characteristic ion peak of Mass Spectrometer Method corresponding with drugs is obtained；
(A2) saliva of drug addict is accused of in collection；
(A3) extractant is added in saliva, extracts and stand；
(A4) Aspirate supernatant；
(A5) supernatant is detected using mass spectrograph, obtains the intensity at the characteristic ion peak；
(A6) it is accused of whether drug addict takes drugs according to the intensity, threshold decision.
Compared with prior art, the device have the advantages that being：
1. or simple sample pretreatment need not only be needed, tediously long chromatogram preprocessing process is saved, shortens sample analysis time；
Add the extraction of the extractants such as proper amount of methanol into sample, stand, sample pretreatment can be completed in a few minutes；
2. single detection price is low, cost≤1 yuan so that batch samples detection is possibly realized；
3. detection sensitivity is high, anti matrix effect is good, high throughput analysis can be carried out under open type environment；
4. by the corresponding relation between establishing characteristic ion peak intensity and taking drugs, so that whether take drugs, and drug addict
The interval time of drug abuse, so as to infer time that drug addict consumes illegal drugs the last time.
Brief description of the drawings
Referring to the drawings, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little accompanying drawings are used only for illustrating technical scheme, and are not intended to and protection scope of the present invention is construed as limiting.
In figure：
Fig. 1 is the flow chart of the detection method of according to embodiments of the present invention 1 drug abuse；
Fig. 2 is the mass spectrogram of the saliva of according to embodiments of the present invention 2 drug addict；
Fig. 3 is the mass spectrogram of the saliva of according to embodiments of the present invention 2 another drug addict；
Fig. 4 is the mass spectrogram of the saliva of according to embodiments of the present invention 2 non-drug addict.
Embodiment
Fig. 1-4 and the optional embodiment of the present invention is following description described to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to instruct technical solution of the present invention, simplify or eliminate some conventional aspects.Those skilled in the art
It should be appreciated that modification or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
Following characteristics can combine to form multiple modifications of the present invention in a variety of ways.Thus, the invention is not limited in it is following can
Embodiment is selected, and is only limited by claim and their equivalent.
Embodiment 1：
Fig. 1 schematically illustrates the flow chart of the detection method of the drug abuse of the embodiment of the present invention, as shown in figure 1, described
The detection method of drug abuse comprises the following steps：
(A1) according to influence of the drugs to human body, the characteristic ion peak of Mass Spectrometer Method corresponding with drugs is obtained；Specially：
Using substantial amounts of case, the mass spectrogram of the saliva of the mass spectrogram of the saliva after being taken drugs according to drug addict and non-drug addict
Difference obtain the characteristic ion peak (it is corresponding with these characteristic ion peaks be human body due to metabolin caused by drug abuse, or
Human body is due to by the secretomotor material of drugs), as mass-to-charge ratio be 100,121,129,139,149,163,177,181,228,
237 or 242 quasi-molecular ions, and the intensity at characteristic ion peak and the mapping relations between the time difference are established, the time difference is detection
Time and the time difference for time of taking drugs；
(A2) saliva of drug addict is accused of in collection；
(A3) extractant is added in saliva, such as methanol, formic acid, ethanol, acetic acid or acetonitrile, is extracted and stood；
(A4) capillary Aspirate supernatant is utilized, and capillary is arranged on to the top of the exit direction of ion gun；
(A5) supernatant flowed downward out using ion gun ionization from the capillary, is detected on described using mass spectrograph
Clear liquid, obtain the intensity at the characteristic ion peak；
(A6) it is accused of whether drug addict takes drugs according to the intensity, threshold decision：
The intensity as described in exceedes threshold value, then is judged as taking drugs, and utilizes the intensity and mapping relations, knows drug addict's
Detection time and the time difference for time of taking drugs, so as to deduce the drug abuse time substantially；
Intensity is then judged as not taking drugs not less than threshold value as described in.
Embodiment 2：
The application examples of the detection method of the drug abuse of the embodiment of the present invention 1.
In the application examples, extractant uses methanol, formic acid, ethanol, acetic acid or acetonitrile；Capillary is quartz glass or boron
Capillary glass tube；Ion gun is dielectric barrier discharge ion gun, between the exit portal of the ion gun and mass spectrometric injection port
Line intersects with the central axis of the capillary, and between angle be right angle；It is 100 that characteristic ion peak, which selects mass-to-charge ratio,
121st, 129,139,149,163,177,181,228,237 or 242 quasi-molecular ions.
Fig. 2 schematically illustrates the mass spectrogram of non-drug addict's saliva, and in the mass spectrogram, m/z=100 intensity is
5.05x101。
Fig. 3 schematically illustrates No. 7 mass spectrograms for being accused of drug addict's saliva, in the mass spectrogram, occur it is following from
Sub- peak：m/z：100th, 139,149,177,181,228,237 and 242, wherein m/z=100 intensity is 3.67x102, when being judged as
Between difference be about 3 weeks, actually 25 days；
Fig. 4 schematically illustrates No. 8 mass spectrograms for being accused of drug addict's saliva, in the mass spectrogram, occur it is following from
Sub- peak：m/z：100th, 139,149,177,181,228,237 and 242, wherein m/z=100 intensity is 2.14x103, when being judged as
Between difference about 1 week, actually 9 days.
Claims (10)
1. the detection method taken drugs, the detection method of the drug abuse comprise the following steps：
(A1) according to influence of the drugs to human body, the characteristic ion peak of Mass Spectrometer Method corresponding with drugs is obtained；
(A2) saliva of drug addict is accused of in collection；
(A3) extractant is added in saliva, extracts and stand；
(A4) Aspirate supernatant；
(A5) supernatant is detected using mass spectrograph, obtains the intensity at the characteristic ion peak；
(A6) it is accused of whether drug addict takes drugs according to the intensity, threshold decision.
2. the detection method of drug abuse according to claim 1, it is characterised in that：The mass-to-charge ratio at the characteristic ion peak is
100th, 121,129,139,149,163,177,181,228,237 or 242.
3. the detection method of drug abuse according to claim 1, it is characterised in that：Using capillary Aspirate supernatant, and will
Capillary is arranged on the top of the exit direction of ion gun, the supernatant that the ion gun ionization flows downward out from the capillary
Liquid.
4. the detection method of drug abuse according to claim 3, it is characterised in that：The exit portal and mass spectrograph of the ion gun
Injection port between line intersect with the central axis of the capillary.
5. the detection method of drug abuse according to claim 4, it is characterised in that：Between the line and the central axis
Angle is right angle.
6. the detection method of drug abuse according to claim 1, it is characterised in that：In step (A1), characteristic ion is established
The intensity at peak and the mapping relations between the time difference, the time difference are the time difference of detection time and time of taking drugs；
In step (A6), intensity, the detection time known according to the mapping relations, step (A5) and know the suction of drug addict
The malicious time.
7. the detection method of drug abuse according to claim 6, it is characterised in that：The matter of saliva after being taken drugs according to drug addict
The difference of the mass spectrogram of the saliva of spectrogram and non-drug addict obtains the characteristic ion peak.
8. the detection method of drug abuse according to claim 1, it is characterised in that：The extractant is methanol, formic acid, second
Alcohol, acetic acid or acetonitrile.
9. the detection method of drug abuse according to claim 1, it is characterised in that：The capillary is quartz glass or boron glass
Glass capillary.
10. the detection method of drug abuse according to claim 1, it is characterised in that：The ion gun is dielectric barrier discharge
Ion gun.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710533241.6A CN107561150A (en) | 2017-07-03 | 2017-07-03 | The detection method of drug abuse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710533241.6A CN107561150A (en) | 2017-07-03 | 2017-07-03 | The detection method of drug abuse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107561150A true CN107561150A (en) | 2018-01-09 |
Family
ID=60973545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710533241.6A Pending CN107561150A (en) | 2017-07-03 | 2017-07-03 | The detection method of drug abuse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107561150A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008051520A (en) * | 2006-08-22 | 2008-03-06 | Hitachi Ltd | Medicine detection method and medicine detector |
| CN102903599A (en) * | 2012-10-25 | 2013-01-30 | 公安部第三研究所 | Ion migration tube using surface-desorption atmospheric-pressure chemical ionization technology |
| EP1897014B1 (en) * | 2005-06-30 | 2014-01-15 | BIOCRATES Life Sciences AG | Apparatus for analyzing a metabolite profile |
| CN103884768A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Method for rapidly analyzing saliva poisons |
| CN106198702A (en) * | 2015-05-06 | 2016-12-07 | 中国科学院大连化学物理研究所 | A kind of method of drugs in quick detection saliva |
| CN106841491A (en) * | 2017-03-16 | 2017-06-13 | 北京大学 | A kind of surface plasma body resonant vibration and mass spectrometry device and application method |
-
2017
- 2017-07-03 CN CN201710533241.6A patent/CN107561150A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1897014B1 (en) * | 2005-06-30 | 2014-01-15 | BIOCRATES Life Sciences AG | Apparatus for analyzing a metabolite profile |
| JP2008051520A (en) * | 2006-08-22 | 2008-03-06 | Hitachi Ltd | Medicine detection method and medicine detector |
| CN102903599A (en) * | 2012-10-25 | 2013-01-30 | 公安部第三研究所 | Ion migration tube using surface-desorption atmospheric-pressure chemical ionization technology |
| CN103884768A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Method for rapidly analyzing saliva poisons |
| CN106198702A (en) * | 2015-05-06 | 2016-12-07 | 中国科学院大连化学物理研究所 | A kind of method of drugs in quick detection saliva |
| CN106841491A (en) * | 2017-03-16 | 2017-06-13 | 北京大学 | A kind of surface plasma body resonant vibration and mass spectrometry device and application method |
Non-Patent Citations (1)
| Title |
|---|
| AYANNA U 等: "Analysis of drugs of abuse in biofluids by low temperature plasma(LTP) ionization mass spectrometry", 《ANALYST》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101092371B1 (en) | Simultaneous quantitaive analysis method for tobacco elements and metabolites thereof in human urine | |
| EP2916130B1 (en) | Method for establishing shenqi fuzheng injection fingerprint spectrum | |
| CN103308621B (en) | The method of high flux Liquid Chromatography tandem mass spectrography detection 25(OH)VD | |
| Staub et al. | CE‐TOF/MS: fundamental concepts, instrumental considerations and applications | |
| CN108627595B (en) | Method for simultaneously detecting 12 alkaline drugs in hair | |
| CN108072690B (en) | Ion mobility spectrometry and ion trap mass spectrometry combined device and analysis method | |
| CN107121518B (en) | A kind of method that Sync enrichment detects phenols, estrogens and androgens incretion interferent in drinking water | |
| CN107462625A (en) | A kind of method for building up in the reduction mobility mass spectrometric data storehouse of rapid screening drugs and application | |
| CN104215716B (en) | A kind of detect the method for total testosterone in human serum | |
| CN105181784A (en) | Method for rapidly screening 40 prohibited antibiotics in cosmetics | |
| CN103094050B (en) | A kind of sensitive glow discharge direct ionization method and device thereof | |
| CN112213412A (en) | Method for detecting drug and metabolite thereof in hair | |
| US20150108343A1 (en) | Methods for selective detection of biologically relevant acids | |
| US20140291505A1 (en) | Quantification of an analyte in serum and other biological matrices | |
| US20180166268A1 (en) | Ionization and ion introduction device for mass spectrometer | |
| CN107589184A (en) | The analyzing detecting method and its application of a kind of PEG and PEGylation medicine | |
| Guo et al. | Simultaneous determination of linarin, naringenin and formononetin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Bushen Guchi Pill | |
| CN107192770B (en) | Analytical method for identifying vitex negundo honey and syrup adulterated vitex negundo honey | |
| Lien et al. | Analysis of polycyclic aromatic hydrocarbons by liquid chromatography/tandem mass spectrometry using atmospheric pressure chemical ionization or electrospray ionization with tropylium post‐column derivatization | |
| CN104833761B (en) | Method for quickly analyzing carbohydrates in samples | |
| CN108845063B (en) | Detection reagent combination and detection method of aquatic product additive | |
| CN107561150A (en) | The detection method of drug abuse | |
| CN107422053A (en) | A kind of method of trichloroacetic acid in LC-MS detection cosmetics | |
| CN103293268B (en) | A kind of people urinates the detection method of hydrocortisone | |
| JP2021535387A (en) | Mass correction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180109 |