CN1075594A

CN1075594A - Preparation method for grain spawn of edible fungus

Info

Publication number: CN1075594A
Application number: CN 93101120
Authority: CN
Inventors: 刘进军
Original assignee: Individual
Current assignee: Individual
Priority date: 1993-01-19
Filing date: 1993-01-19
Publication date: 1993-09-01

Abstract

Preparation method for grain spawn of edible fungus, select free from insect pests for use, do not have the grain that goes mouldy, contain in closed container with formalin or " 84 " medicining liquid dipping, treat to pull out after the core tissue soaks into, poach expands, pull out dry, spice, bottling seal, normal-pressure sterilization, inoculated and cultured get grain spawn.The soup that the present invention selects for use has wide spectrum, bactericidal effect efficiently and rapidly, and the source is wide, price is low, easy to use, permeability is strong, and porous is in the grain tissue, and it is volatile to be heated, no residual hazard.Adopt this technology to prepare flat mushroom, mushroom, hedgehog hydnum, Asparagus, glossy ganoderma, the second class inoculum 4 over thousands of bottles of woodear etc., success rate reaches 100%.

Description

Preparation method for grain spawn of edible fungus

The invention belongs to the Edible Fungi technology type, belong to preparation technology's class of grain spawn of edible fungus particularly.

In recent years, with cereal kernel (with iblet the best, wheat, paddy also can) on Edible Fungi, obtained good effect as the made bacterial classification of medium, performance obviously is better than the bacterial classification that other materials is made, so grain spawn is at home and abroad generally promoted the use of.

The success or failure of grain spawn preparation depend on whether the inner sterilization of grain is thorough.The sterilizing methods that generally adopts is the autoclave sterilization method now, promptly sterilizes 2-3 hour under 126 ℃ of temperature.Though HTHP can be killed the harmful microorganism of grain inside, make the nutrient component in the medium be subjected to very havoc simultaneously.Autoclaving also need adopt high-pressure sterilizing pot in addition.Small-sized autoclave treating capacity is little, does not satisfy the production needs; Large-scale sterilizing pot valency is expensive, energy consumption big, operation is strict, be difficult for popularization.

At the problems referred to above, people's normal-pressure sterilization method that begins one's study, show the disclosed methods such as " carbendazim " class agricultural chemicals and the immersion of limewash soup that have, the technology that adopts these methods to prepare bacterial classification is: select free from insect pests earlier for use, do not have the grain that goes mouldy, contain in closed container, soak with soup, remove the assorted bacterium of grain inside; Treat to pull out after grain core tissue soaks into, clear water is eluriated, and adds poach, pulls out and dry; Spice is again admixed the calcium carbonate of grain dry weight 1-2%, and mixing thoroughly promptly becomes medium; Adopt the normal-pressure sterilization method after refilling bottle closure, kill the assorted bacterium that media surface may be infected with; Inoculate cultivate grain spawn.

Soak the growth that the grain sterilization can only temporarily suppress the minority mould with limewash, and brood body or hypopus be there is no killing action, some harmful microorganism is not had killing action yet, and grain is under alkaline limewash soaks, most of nutrient component of its inside is also destroyed.

Soak grain with " carbendazim " class agricultural chemicals and have following problem:

1, " carbendazim " class agricultural chemicals is only effective to some sac fungis and imperfect fungus, and most harmful microorganism (as Mucor, head mold, bacterium, virus etc.) be there is no drug effect.When using the cultivation bacterium is also produced inhibitory action, all very responsive as woodear, Hericium erinaceus, Ganderma lucidum etc. to it, do not allow to use;

2, the heat endurance of " carbendazim " class agricultural chemicals is extremely strong, and high temperature reaches 250 ℃ and also is difficult for decomposing, and the residual medicine phase is longer, easily develops immunity to drugs.

3, " carbendazim " class agricultural chemicals is wetting powder or emulsion, and the solvability in water is low, can not penetrate into the interior tissue of grain well, and the actual effect of soaking is relatively poor.

The objective of the invention is deficiency, a kind of effective normal pressure grain sterilizing methods is provided, adopt this method can prepare grain spawn reliably in a large number at above-mentioned all sterilizing methods existence.Adopt this technology, equipment needed thereby is simple, processing ease, preparation amount is big, success rate is high, cost is low.

The objective of the invention is to realize in the following manner; preparation method for grain spawn of edible fungus; select free from insect pests earlier for use, do not have the grain (iblet, wheat or paddy) that goes mouldy; contain again in closed container and soak sterilization with soup; treat to pull out after grain core tissue soaks into; clear water is eluriated, poach expands, pull out dry, spice gets medium, bottling is sealed, normal-pressure sterilization, inoculated and cultured get grain spawn.Pull out after will grain floating the impurity elimination thing with clear water through selecting for use, in the Sheng Yuke sealing soaking container, in container, inject bactericidal liquid, consumption is generally 2 times of grain dry weight, under the stirred for several, with container closure, bactericidal liquid is formalin or " 84 " thimerosal, formalin is that the formaldehyde of chemical pure level adds 80-120 water dilution doubly and forms, and " 84 " thimerosal forms for " 84 " liquid adds 100-200 water dilution doubly.Pull the land plaster of admixing grain dry weight 0.5% after drying and 0.5% kemidol out.

Formalin (being formalin) is a kind of widely used water-soluble strong fungicide, permeate well, and volatile when being heated, residual medicine is low.Formaldehyde also can make the crude protein sex change in the grain, is convenient to be decomposed, absorb by mycelium.

" 84 thimerosals are a kind of novel wide spectrum, efficient, quick-acting bactericide, and harmful microorganisms such as mould, bacterium, virus are had good killing action, can decompose rapidly when being heated, and substrate is sodium chloride and water, no residual hazard." 84 " liquid contains surfactant component, soup can be penetrated in the grain tissue smoothly.

It is sterilized solution that the present invention selects above two kinds of soups for use, and the source is wide, price is low, easy to use.In use can be a kind of with selecting according to temperature conditions, general temperature be higher than 18 ℃ better with formalin, be lower than 20 ℃ and be advisable with " 84 " thimerosal.

After treating that grain core tissue soaks into, pull out with clear water and eluriate, remove soup and impurity.Put into the water that has boiled again, continued to boil 15-30 minute, treat the grain imbibition after, pull out while hot, dry, make the further dissipation of residual medicine, again spice.

Biology department of Wuhan University once carried out 20 test of many times with technology of the present invention, adopt the native steamer of the diesel bucket transformation of the way of haveing been friends in the past, the second class inoculum 4 over thousands of bottles that prepare flat mushroom, mushroom, hedgehog hydnum, Asparagus, glossy ganoderma, woodear etc. altogether, the blank cultivation of sampling reaches 30 days and does not also find varied bacteria growing, and success rate reaches 100%.Bacterial classification is dropped into the actual production checking, and effect has met or exceeded the bacterial classification with the preparation of autoclaving processing method fully.

Claims

1; preparation method for grain spawn of edible fungus; select free from insect pests earlier for use; there is not the grain (iblet that goes mouldy; wheat or paddy); contain in closed container innerlich anwenden liquid and soak sterilization; treat to pull out after grain core tissue soaks into; clear water is eluriated; poach expands; pull out and dry; spice gets medium; bottling is sealed; normal-pressure sterilization; inoculated and cultured gets grain spawn; pull out after it is characterized in that will grain floating the impurity elimination thing with clear water through selecting for use; contain in closed container; in container, inject formalin or " 84 " thimerosal bactericidal liquid; consumption is generally 2 times of grain dry weight; under the stirred for several, land plaster and 0.5% kemidol of admixing grain dry weight 0.5% after closed container immersion poach dries rapidly get medium.

2, technology according to claim 1 is characterized in that formalin is that the formaldehyde of chemical pure level adds 80-120 water dilution doubly and forms.

3, technology according to claim 1 is characterized in that " 84 " thimerosal forms for " 84 " liquid adds 100-200 water dilution doubly.