CN107550849A - A kind of madecassoside lipidosome gel of local topical and preparation method thereof - Google Patents
A kind of madecassoside lipidosome gel of local topical and preparation method thereof Download PDFInfo
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- CN107550849A CN107550849A CN201710805294.9A CN201710805294A CN107550849A CN 107550849 A CN107550849 A CN 107550849A CN 201710805294 A CN201710805294 A CN 201710805294A CN 107550849 A CN107550849 A CN 107550849A
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Abstract
The present invention discloses madecassoside lipidosome gel of a kind of local topical and preparation method thereof.The lipidosome gel is mainly made up of madecassoside liposome, gel and ultra-pure water；The liposome has bilayer structure, and the madecassoside is encapsulated in liposome；The quality of the madecassoside liposome accounts for the 1~60% of lipidosome gel gross mass, and the quality of the gel accounts for the 1~30% of lipidosome gel gross mass.The characteristics of its semi-solid gel state of the lipidosome gel, medicine have fitting effect well in wound, ensure that administration time, medicament slow release effect is preferably played, reaches more preferable antibacterial and anti-inflammation functions, is more beneficial for wound healing, and have no stimulation of the skin, highly effective and safe；And liposome can be promoted effectively to pass through skin so that more madecassoside components stays become apparent from skin corium, drug effect.As a kind of excellent reparation scar and wound healing sustained release preparation can be promoted.
Description
Technical field
The invention belongs to pharmaceutical preparation and skin care implement field, and in particular to a kind of madacasso grass of local topical
Glycosides lipidosome gel and preparation method thereof and the application on treatment scar.
Background technology
Madecassoside is main three in samphire centella (Centella asiatica (L.) Urban)
One of terpene saponin constituent.Madecassoside has the extensive pharmacological activity such as anti-inflammatory, anti-oxidant, promotion wound healing, uses
In treatment skin injury, empyrosis and repair scar.Because of the characteristics of being limited to its polarity macromolecular, madecassoside is difficult straight
Connect and pass through keratoderma and epidermis.
Liposome is a kind of to be similar to cell membrane, the orbicule being made up of phosphatide dual sub-layers.The inside of liposome can be with
Various fat-soluble or water miscible medicine is wrapped up, and then improves the stability and utilization rate of medicine.It is enclosed with water soluble drug
Liposome, skin can be passed through by cell intermembrane space, and then can also be delivered drugs into intracellular with cell membrane fusion
Portion.Liposome solutions, because its mobility is strong, are easy to be lost in when treating skin wound, cause the waste of medicine, and then increase
Dermatologic amount.
Gel is the semisolid or thick liquid preparation for having gel characteristic.Gel is typically adjusted by gel-type vehicle, pH
Save the composition such as agent, transdermal enhancer and NMF.The method for preparing gel is simple to operate, and skin is hindered when locally using
Evil is small, there is preferable stickiness and ductility, and possesses the characteristic that control medicine slowly discharges, and extends administration time, shortens skin
Skin healing time.
The content of the invention
The shortcomings that in order to overcome prior art, is to provide a kind of hydroxyl of local topical with deficiency, primary and foremost purpose of the invention
Base asiaticosid lipidosome gel.To make madecassoside give full play to the effect of repairing scar, wound healing, the present invention carries
For a kind of lipidosome gel.
Lipidosome gel of the present invention uses nano-lipid Antibody Production Techniques, madecassoside is encapsulated in lipid
In body, add gel substrate and ultra-pure water forms semi-solid gel state medicine.Its semi-solid gel of the lipidosome gel
The characteristics of state, medicine have fitting effect well in wound, ensure that administration time, preferably play medicament slow release effect,
Reach more preferable antibacterial and anti-inflammation functions, be more beneficial for wound healing；On the other hand, the lipidosome gel can promote liposome effective
Through skin so that more madecassoside components stays become apparent from skin corium, drug effect.
Another object of the present invention is to the preparation side for the madecassoside lipidosome gel for providing above-mentioned local topical
Method.
It is strong for liposome aqueous solution mobility, as pharmaceutical carrier, the problem of skin is easily lost in is applied to, passes through this
Invention prepares the good madecassoside liposome gel of skin attachement.Madecassoside lipidosome gel in the present invention
Agent is realized by following steps：Penetrating agent, NMF and gel are first added in ultra-pure water, is sufficiently stirred, blank is formed and coagulates
Glue, madecassoside liposome solutions are then added, adjust pH value, stir, be made that stability is strong, fitting effect is good
Madecassoside lipidosome gel.
It is still another object of the present invention to provide the application of the madecassoside lipidosome gel of above-mentioned local topical.
The purpose of the present invention is achieved through the following technical solutions：
A kind of madecassoside lipidosome gel of local topical, the lipidosome gel is mainly by madecassoside
Liposome, gel and ultra-pure water composition；The liposome has bilayer structure, and the madecassoside is encapsulated in fat
In plastid；The quality of the madecassoside liposome accounts for the 1~60% of lipidosome gel gross mass, the matter of the gel
Amount accounts for the 1~30% of lipidosome gel gross mass.
Preferably, the quality of the madecassoside liposome accounts for the 20% of lipidosome gel gross mass, the gel
The quality of agent accounts for the 6% of lipidosome gel gross mass；
The preparation method of the madecassoside lipidosome gel of above-mentioned local topical, comprises the following steps：
(1) madecassoside liposome solutions are prepared；
(2) Blank gel is prepared：Penetrating agent, NMF and gel substrate are added in ultra-pure water, is sufficiently stirred, is formed
Blank gel；
(3) madecassoside liposome solutions are added in the Blank gel obtained to step (2), pH adjusting agent regulation
PH value, the madecassoside lipidosome gel of uniform gel, i.e. local topical is stirred into, is placed at 4 DEG C and preserves.Described
Madecassoside lipidosome gel stability is good, evenness, and quality is fine and smooth, and stickiness is moderate.
Preferably, in step (1),
In madecassoside liposome solutions, the quality of madecassoside accounts for the 0.1~60% of gross mass, phosphatide
Quality accounts for the 10~90% of gross mass；Further, in madecassoside liposome solutions, the quality of madecassoside accounts for
The 1.41~22.22% of gross mass, the quality of phosphatide account for the 55.56~70.42% of gross mass；
The preparation method of madecassoside liposome solutions is film dispersion method, alcohol injection, freeze-drying, breast
Change method；Preferably emulsion process；Madecassoside solution concentration is 1~160mg/mL, preferably 1~20mg/mL, is more preferably
20mg/mL。
Take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, add appropriate antioxidant, filter, rotary evaporation removes
Then organic solvent adds the certain density madecassoside aqueous solution or PBS solution, added after bead at a high speed into film
Stirring or direct ultrasonication, obtain suspension, suspension can further use filtering with microporous membrane, produce madecassoside
Liposome solutions；
Or take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, appropriate antioxidant is added, is filtered, rotary evaporation
Organic solvent is removed into film, the finite concentration madecassoside aqueous solution or PBS solution are added in above-mentioned organic solvent, added
Enter appropriate freeze protection agent, mix, vacuum freeze drying, produce madecassoside liposome solutions.
Or take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, it is injected into what is prepared certain density
In the madecassoside aqueous solution or PBS solution, it is added dropwise while stir, while organic solvent is removed under reduced pressure, is suspended
Liquid, suspension can further use filtering with microporous membrane, produce madecassoside liposome solutions.
Or mass ratio takes phosphatide and cholesterol to be dissolved in organic solvent, appropriate antioxidant is added, adds finite concentration hydroxyl
The base asiaticosid aqueous solution or PBS solution, emulsification forms W/O emulsions under certain condition, then above-mentioned W/O emulsions are then added to
In the certain density madecassoside aqueous solution or PBS solution, emulsify, be evaporated under reduced pressure after emulsion under certain condition again
Organic solvent is removed, filtering with microporous membrane can be used, produce madecassoside liposome solutions.
Described phosphatide is natural phospholipid or synthetic phospholipid；Preferably egg yolk lecithin.
Described phosphatide and the mass ratio of cholesterol are 1:1~10:1.
Described antioxidant is vitamin C or Vitwas E.
Described organic solvent is dichloromethane, chloroform, n-hexane, hexamethylene, ether etc., preferably dichloromethane.
Preferably, in step (2),
Penetrating agent is azone, menthol, Laurocapram or PVP, preferably azone, and whole quality is divided
Number is 0.1~5%, preferably 1%；NMF is glycerine or propane diols, preferably propane diols, and whole mass fraction is 1~50%,
Preferably 15%；Gel substrate is sodium carboxymethylcellulose, carbomer, sodium alginate, hydroxypropyl methylcellulose etc., preferably carboxylic
Sodium carboxymethylcellulose pyce, whole mass fraction are 1~30%, preferably 1~10%, more preferably 4~6%, most preferably 6%.
Preferably, in step (3), regulation pH value is 5~9, and preferably pH is 7.
Application of the madecassoside lipidosome gel of above-mentioned local topical in medicine and skin care implement field.
Preferably, application of the madecassoside lipidosome gel of the local topical on treatment scar,
Preferably, the madecassoside lipidosome gel of the local topical is being repaired scar and promoted in wound healing
Application.
The present invention is had the following advantages relative to prior art and effect：
(1) madecassoside lipidosome gel envelop rate of the invention can reach more than 70%, preferably 70.2~
79%, SD burned rats experimental results are shown within 15 days, and the healing effect of madecassoside lipidosome gel group is substantially better than sky
White control group.Madecassoside lipidosome gel has significant slow release effect, and medicine works well in wound surface fitting,
And have no stimulation of the skin, highly effective and safe, solve madecassoside solution or its liposome solutions in percutaneous drug delivery mistake
Cheng Zhong, the problem of mobility is strong, stability is poor, drug availability is low, as a kind of excellent reparation scar and wound can be promoted
Heal sustained release preparation.
(2) invention formulation and naked medicine phases ratio, have obvious slow release effect, and treatment cycle shortens；Solves hydroxyl product
The problems such as Asiaticoside solution or its liposome solutions mobility are strong, skin attachement effect difference；It can be cured as a kind of excellent wound
Close, repair scar external application sustained release agent.
Brief description of the drawings
Fig. 1 is the particle size distribution figure of madecassoside liposome made from embodiment 2.
Fig. 2 is madecassoside lipidosome gel outside drawing under different sodium carboxymethylcellulose dosages；Wherein, A：2%
Sodium carboxymethylcellulose (embodiment 1)；B：4% sodium carboxymethylcellulose (embodiment 2)；C：6% sodium carboxymethylcellulose (is implemented
Example 3).
Fig. 3 is comparison diagram before and after the centrifugation of madecassoside lipidosome gel；Wherein, A：Before centrifugation；B：After centrifugation.
Fig. 4 is that madecassoside lipidosome gel is applied to SD rat skin design sketch.
Fig. 5 is blank control group and madecassoside lipidosome gel group SD rat skin scalding healing Contrast on effect
Figure.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
Embodiment 1
(1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetic acid
Ester 0.1g, which is dissolved in 10mL dichloromethane, is configured to organic solution；Rotary evaporation removes organic solvent into film, by 20mg/
ML madecassosides solution is added in film, is added at appropriate 50 DEG C of bead and is stirred 1h, Probe Ultrasonic Searching 30min, after
0.22 μm of water-based filter membrane, obtains madecassoside liposome solutions.In the madecassoside liposome solutions, madacasso
The quality of careless glycosides accounts for 22.22%, and the quality of egg yolk lecithin accounts for 55.56%.
(2) Blank gel is prepared：Mass fraction 0.1g azones are added in 8mL water, 1.5g propane diols, add 0.2g's
Sodium carboxymethylcellulose, it is sufficiently stirred, obtains Blank gel agent.
(3) 2mL madecassosides liposome solutions in step (1) are added in step (2) in Blank gel agent, fully
Stir, madecassoside lipidosome gel is both obtained for 7 with 5% NaOH solution regulation pH value.Madecassoside fat
Plastid gel form appearance is as shown in Figure 2.
The envelop rate of the madecassoside lipidosome gel is 71%.
Embodiment 2
(1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetic acid
Ester 0.1g, which is dissolved in 10mL ethanol, is configured to organic phase solution；15mg/mL hydroxyls are added dropwise in above-mentioned organic phase solution
In asiaticosid solution, it is added dropwise while stir until solvent is cleared, crosses 0.22 μm of oiliness filter membrane, obtain madecassoside fat
Plastid solution.In the madecassoside liposome solutions, the quality of madecassoside accounts for 17.65%, egg yolk lecithin
Quality account for 58.82%.
(2) Blank gel is prepared：Mass fraction 0.1g azones are added in 7.5mL water, 2.0g propane diols, add 0.4g
Sodium carboxymethylcellulose, be sufficiently stirred, obtain Blank gel agent.
(3) 2.5mL madecassosides liposome solutions in step (1) are added in step (2) in Blank gel agent, used
7% NaOH solution regulation pH value both obtains madecassoside lipidosome gel for 7.Obtained madecassoside liposome
Average grain diameter is 200nm, as shown in Figure 1.Madecassoside lipidosome gel form appearance is as shown in Figure 2.
The envelop rate of the madecassoside lipidosome gel is 73%.
Embodiment 3
(1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetic acid
Ester 0.1g, which is dissolved in 10mL dichloromethane, is configured to organic solution；Rotary evaporation removes organic solvent into film, by 10mg/
ML madecassosides solution is added in film, and rotation aquation obtains liposome solutions, adds 0.005g mannitol, mixes, vacuum
Middle freeze-drying, lipidosome freeze-dried powder is shaken with PBS and disperseed, obtains madecassoside liposome solutions.The madacasso
In careless glycosides liposome solutions, the quality of madecassoside accounts for 12.42%, and the quality of egg yolk lecithin accounts for 62.11%.
(2) Blank gel is prepared：Mass fraction 0.1g azones are added in 7mL water, 1.8g propane diols, add 0.6g's
Sodium carboxymethylcellulose, it is sufficiently stirred, obtains Blank gel agent.
(3) 2mL madecassosides liposome solutions in step (1) are added in step (2) in Blank gel agent, used
7% NaOH solution regulation pH value both obtains madecassoside lipidosome gel for 7.Obtained madecassoside liposome
Average grain diameter is 180nm, and the madecassoside lipidosome gel outward appearance that different gel doses are prepared is as shown in Figure 2.
As can be known from Fig. 2, the gel mobility that prepared by the sodium carboxymethylcellulose of final concentration 2% is still very strong, and concentration
Gel mobility for 4% and 6% sodium carboxymethylcellulose preparation is far short of what is expected, and concentration is 6% sodium carboxymethylcellulose system
Standby gel effect is best, and illiquidity is strong, and adhesion is big.
The envelop rate of the madecassoside lipidosome gel is 78%.
Embodiment 4
(1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetic acid
Ester 0.1g, which is dissolved in 10mL dichloromethane, is configured to organic phase solution；20mg/mL madecassoside solution is dissolved in
State in organic phase solution；3min is stirred with 500 turns/min for the first time, forms W/O emulsions；Above-mentioned W/O emulsions are added to 20mg/
In mL madecassoside solution, 5min is stirred with 500 turns/min for the second time, forms W/O/W emulsions；Emulsion is transferred to eggplant shape
In bottle, it is evaporated under reduced pressure by Rotary Evaporators and removes organic solvent；Eggplant-shape bottle is put into vacuum drying chamber again and dried, removes breast
Remaining organic solvent in liquid, after ultrasonic 60s, 0.22 μm of water-based filter membrane is crossed, madecassoside liposome solutions is obtained, is placed in
Preserved at 4 DEG C.In the madecassoside liposome solutions, the quality of madecassoside accounts for 22.22%, egg yolk lecithin
Quality account for 55.56%.
(2) madecassoside lipidosome gel is prepared：Mass fraction 0.1g azones, 2.5g the third two are added in 8mL water
Alcohol, 0.6g sodium carboxymethylcellulose is added, is sufficiently stirred, obtain Blank gel agent；Then 2mL madecassosides are added
Liposome solutions, regulation pH value both obtain madecassoside lipidosome gel for 7.The madecassoside lipidosome gel
Envelop rate is 79%.
Madecassoside lipidosome gel does not have lamination after high speed centrifugation, and stability is good, as shown in Figure 3.
Wound skin surface fitting effect is as shown in Figure 4, the results showed that the lipidosome gel prepared under the conditions of being somebody's turn to do is in skin
The fitting effect of wound surface is good, is slowly discharged on wound beneficial to medicine.
Embodiment 5
1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetate
0.1g, which is dissolved in 10mL chloroforms, is configured to organic solution；Rotary evaporation removes organic solvent into film, by 5mg/mL
Madecassoside solution is added in film, is added at appropriate 50 DEG C of bead and is stirred 1h, Probe Ultrasonic Searching 30min, after 0.22 μ
The water-based filter membranes of m, obtain madecassoside liposome solutions.In the madecassoside liposome solutions, madecassoside
Quality account for 3.45%, the quality of egg yolk lecithin accounts for 68.97%.
(2) Blank gel is prepared：Mass fraction 0.1g azones are added in 8mL water, 1.5g propane diols, add 0.2g's
Sodium carboxymethylcellulose, it is sufficiently stirred, obtains Blank gel agent.
(3) 2mL madecassosides liposome solutions in step (1) are added in step (2) in Blank gel agent, fully
Stir, madecassoside lipidosome gel is both obtained for 7 with 5% NaOH solution regulation pH value.Madecassoside fat
Plastid gel form appearance is as shown in Figure 2.
The envelop rate of the madecassoside lipidosome gel is 70.2%.
Embodiment 6
(1) madecassoside liposome solutions are prepared：Weigh egg yolk lecithin 0.50g, cholesterol 0.10g, VE acetic acid
Ester 0.1g, which is dissolved in 10mL dichloromethane, is configured to organic phase solution；1mg/mL madecassoside solution is dissolved in above-mentioned
In organic phase solution；3min is stirred with 500 turns/min for the first time, forms W/O emulsions；Above-mentioned W/O emulsions are added to 1mg/mL
In madecassoside solution, 5min is stirred with 500 turns/min for the second time, forms W/O/W emulsions；Emulsion is transferred to eggplant-shape bottle
In, it is evaporated under reduced pressure by Rotary Evaporators and removes organic solvent；Eggplant-shape bottle is put into vacuum drying chamber again and dried, removes emulsion
In remaining organic solvent, after ultrasonic 60s, cross 0.22 μm of water-based filter membrane, obtain madecassoside liposome solutions, be placed in 4
Preserved at DEG C.In the madecassoside liposome solutions, the quality of madecassoside accounts for 1.41%, egg yolk lecithin
Quality accounts for 70.42%.
(2) madecassoside lipidosome gel is prepared：Mass fraction 0.1g azones, 2.5g the third two are added in 8mL water
Alcohol, 0.6g sodium carboxymethylcellulose is added, is sufficiently stirred, obtain Blank gel agent；Then 2mL madecassosides are added
Liposome solutions, regulation pH value both obtain madecassoside lipidosome gel for 7.The madecassoside lipidosome gel
Envelop rate is 72%.
Madecassoside lipidosome gel does not have lamination after high speed centrifugation, and stability is good, as shown in Figure 3.
Wound skin surface fitting effect is as shown in Figure 4, the results showed that the lipidosome gel prepared under the conditions of being somebody's turn to do is in skin
The fitting effect of wound surface is good, is slowly discharged on wound beneficial to medicine.
Embodiment 7
Rat two level scalding model is established：SD rats 2 (about 300g) are taken, shave off rat back hair after anesthesia, then with 9%
Sodium sulfide solution hair is cleared.A diameter of 1.4cm screw is put into after being heated 1 minute in boiling water (about 100 DEG C), be placed in old
Mouse back keeps 20s, and to form the wound of two level scald, every rat back makes 6 wounds altogether, as Duplicate Samples.Then yarn is used
Cloth is wrapped up to wound to rat forms a scab.
2 rats are divided into blank control group and madecassoside lipidosome gel group, wherein blank control group uses
PBS, medicine-feeding daily is sooner or later twice, and photographed to record sight with measurement wound area at the 0th, 3,6,9,12 and 15 day
Examine the change of wound.
Fig. 5 can be seen that the rat wound of madecassoside lipidosome gel group was obviously reduced at the 6th to 9 day, healing
Effect is best.Good fitting effect adds the release time of madecassoside liposome, plays the effect of continued administration,
Madecassoside is set preferably to play anti-inflammatory, wound healing, repair scar and other effects.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. the madecassoside lipidosome gel of a kind of local topical, it is characterised in that the lipidosome gel is mainly by hydroxyl
Asiaticosid liposome, gel and ultra-pure water composition；The liposome has bilayer structure, the madecassoside
It is encapsulated in liposome；The quality of the madecassoside liposome accounts for the 1~60% of lipidosome gel gross mass, described solidifying
The quality of jelly accounts for the 1~30% of lipidosome gel gross mass.
2. the madecassoside lipidosome gel of local topical according to claim 1, it is characterised in that：
In described madecassoside liposome, the quality of madecassoside accounts for the 0.1~60% of gross mass, the matter of phosphatide
Amount accounts for the 10~90% of gross mass.
3. the preparation method of the madecassoside lipidosome gel of the local topical described in claim 1 or 2, it is characterised in that
Comprise the following steps：
(1) madecassoside liposome solutions are prepared；
(2) Blank gel is prepared：Penetrating agent, NMF and gel substrate are added in ultra-pure water, is sufficiently stirred, forms blank
Gel；
(3) madecassoside liposome solutions are added in the Blank gel obtained to step (2), pH adjusting agent regulation pH
Value, stir into the madecassoside lipidosome gel of uniform gel, i.e. local topical.
4. preparation method according to claim 3, it is characterised in that：
In step (1), the preparation method of madecassoside liposome solutions is film dispersion method, alcohol injection, is freezed dry
Dry method, emulsion process.
5. the preparation method according to claim 3 or 4, it is characterised in that：
In step (1), the preparation method of madecassoside liposome solutions, comprise the following steps：
Take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, add appropriate antioxidant, filter, rotary evaporation removes organic
Then solvent adds the certain density madecassoside aqueous solution or PBS solution, adds high-speed stirred after bead into film
Or direct ultrasonication, suspension is obtained, suspension further uses filtering with microporous membrane, produces madecassoside liposome
Solution；
Or take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, appropriate antioxidant is added, is filtered, rotary evaporation removes
The finite concentration madecassoside aqueous solution or PBS solution are added in above-mentioned organic solvent, added suitable into film by organic solvent
Freeze protection agent is measured, mixes, vacuum freeze drying, produces madecassoside liposome solutions；
Or take phosphatide and cholesterol to be dissolved in organic solvent in mass ratio, it is injected into the certain density hydroxyl prepared
In the asiaticosid aqueous solution or PBS solution, it is added dropwise while stir, while organic solvent is removed under reduced pressure, obtains suspension, mixes
Suspension further uses filtering with microporous membrane, produces madecassoside liposome solutions；
Or mass ratio takes phosphatide and cholesterol to be dissolved in organic solvent, appropriate antioxidant is added, adds finite concentration hydroxyl product
The Asiaticoside aqueous solution or PBS solution, emulsification forms W/O emulsions under certain condition, then above-mentioned W/O emulsions are then added to necessarily
In the madecassoside aqueous solution or PBS solution of concentration, emulsify under certain condition again, be evaporated under reduced pressure and remove after emulsion
Organic solvent, with filtering with microporous membrane, produce madecassoside liposome solutions.
6. preparation method according to claim 3, it is characterised in that：
In step (2),
The whole mass fraction of penetrating agent is 0.1~5%；
The whole mass fraction of NMF is 1~50%；
The whole mass fraction of gel is 1~30%.
7. the preparation method according to claim 3 or 6, it is characterised in that：
In step (2),
Penetrating agent is azone, menthol, Laurocapram or PVP；
NMF is glycerine or propane diols；
Gel is sodium carboxymethylcellulose, carbomer, sodium alginate, hydroxypropyl methylcellulose.
8. preparation method according to claim 3, it is characterised in that：
In step (3), regulation pH value is 5~9.
9. the madecassoside lipidosome gel of the local topical described in claim 1 or 2 is in medicine and skin care implement
The application in field.
10. the application sent out according to claim 9, it is characterised in that：
Application of the madecassoside lipidosome gel of the local topical on treatment scar.
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| CN107823695A (en) * | 2017-09-19 | 2018-03-23 | 华东理工大学 | A kind of intelligent antiseptic dressing and preparation method thereof |
| CN108969797A (en) * | 2018-07-23 | 2018-12-11 | 武汉理工大学 | A kind of load lipidosome gel and preparation method thereof with electro photoluminescence and the neural scar effect of inhibition |
| CN109999070A (en) * | 2018-04-27 | 2019-07-12 | 西南大学 | A kind of asiaticoside liposome and preparation process |
| CN112121003A (en) * | 2020-09-29 | 2020-12-25 | 江苏集萃新型药物制剂技术研究所有限公司 | Drug-carrying material of sustained-release preparation, composition of drug-carrying material, sustained-release preparation and preparation method of sustained-release preparation |
| CN112316205A (en) * | 2020-11-16 | 2021-02-05 | 中国药科大学 | External asiaticoside gel dressing and preparation method thereof |
| CN113209007A (en) * | 2021-05-17 | 2021-08-06 | 海南普利制药股份有限公司 | Asiaticoside preparation for external use and its preparation method |
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| CN113713000A (en) * | 2021-10-19 | 2021-11-30 | 江苏集萃新型药物制剂技术研究所有限公司 | Main medicine component composition for treating sore carbuncle, burn, scald and acne, sustained and controlled release pharmaceutical preparation, and preparation method and application thereof |
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| CN107823695B (en) * | 2017-09-19 | 2022-03-15 | 华东理工大学 | Intelligent antibacterial dressing and preparation method thereof |
| CN109999070A (en) * | 2018-04-27 | 2019-07-12 | 西南大学 | A kind of asiaticoside liposome and preparation process |
| CN108969797A (en) * | 2018-07-23 | 2018-12-11 | 武汉理工大学 | A kind of load lipidosome gel and preparation method thereof with electro photoluminescence and the neural scar effect of inhibition |
| FR3108037A1 (en) * | 2020-03-10 | 2021-09-17 | Farevacare | Cosmetic or dermatological composition for the maintenance of the skin microbiome |
| CN112121003A (en) * | 2020-09-29 | 2020-12-25 | 江苏集萃新型药物制剂技术研究所有限公司 | Drug-carrying material of sustained-release preparation, composition of drug-carrying material, sustained-release preparation and preparation method of sustained-release preparation |
| CN112121003B (en) * | 2020-09-29 | 2022-02-01 | 江苏集萃新型药物制剂技术研究所有限公司 | Drug-carrying material of sustained-release preparation, composition of drug-carrying material, sustained-release preparation and preparation method of sustained-release preparation |
| CN112316205A (en) * | 2020-11-16 | 2021-02-05 | 中国药科大学 | External asiaticoside gel dressing and preparation method thereof |
| CN113209007A (en) * | 2021-05-17 | 2021-08-06 | 海南普利制药股份有限公司 | Asiaticoside preparation for external use and its preparation method |
| CN113713000A (en) * | 2021-10-19 | 2021-11-30 | 江苏集萃新型药物制剂技术研究所有限公司 | Main medicine component composition for treating sore carbuncle, burn, scald and acne, sustained and controlled release pharmaceutical preparation, and preparation method and application thereof |
| CN113713000B (en) * | 2021-10-19 | 2022-03-22 | 江苏集萃新型药物制剂技术研究所有限公司 | Main medicine component composition for treating sore carbuncle, burn, scald and acne, sustained and controlled release pharmaceutical preparation, and preparation method and application thereof |
| CN115105629A (en) * | 2022-07-26 | 2022-09-27 | 暨南大学 | Antibacterial hydrogel and preparation method and application thereof |
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