CN107537055A - A kind of preparation and its application of Thermo-sensitive hydroxyl butyl chitosan protective dressing - Google Patents
A kind of preparation and its application of Thermo-sensitive hydroxyl butyl chitosan protective dressing Download PDFInfo
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Abstract
The present invention relates to a kind of preparation and its application of Thermo-sensitive hydroxyl butyl chitosan protective dressing, comprise the following steps：First, the preparation of Thermo-sensitive hydroxyl butyl chitosan：Chitosan purifying products, by chitosan crude product through certain purifying, wash and be dried to obtain；Chitosan after purification is handled through strong base solution, alkylated reaction, washing are dried to obtain；2nd, the preparation of Thermo-sensitive hydroxyl butyl chitosan protective dressing, take hydroxyl butyl chitosan raw material made from previous step, 0.1% ~ 20% aqueous solution is configured in mass ratio, add about 8mg/ml sodium chloride, about 0.5 mg/ml disodium hydrogen phosphates, about 0.15 mg/ml sodium dihydrogen phosphates, it is placed in certain container and is made through processes such as coarse filtration, refined filtration, embedding, sterilizing, outer packings.The antibacterial that product of the present invention has hinders bacterium, quick-acting haemostatic powder analgesic, keeps surface of a wound moistening, good air permeability, promotes cell tissue growth, accelerates Wound healing, not with tissue adhesion, the characteristics such as biocompatibility and degradability are good.Product of the present invention to various operative incisions, burn, scald, scratch, ulcer wound surface, bedsore, take the dermatotome surface of a wound and various surfaces, traumatic trauma surface infestation, the nursing of neonate's pars muscularis all has the effect of fine.
Description
Technical field
The present invention relates to medical product field, specifically, the present invention relates to a kind of preparation with Thermo-sensitive hydroxyl butyl chitosan protective dressing and its application, product of the present invention has the advantages that antibacterial resistance bacterium, promotes wound healing, be quick-acting haemostatic powder analgesic, fine air permeability, not good with tissue adhesion, biocompatibility.
Background technology
Wound dressing is a kind of medical material for temporary covering wound, and its basic function is to control the diffusate and protection wound of wound, organizes the pollution from bacterium and grit, there is provided be advantageous to the environment of wound healing.With the development of modern medical science and the continuous improvement of living standard, people propose higher requirement to wound dressing, no longer meet that there is the effect of single with traditional dressing such as gauze, single-coated foam etc., it is desirable to which developing a kind of has novel and multifunctional medical dressing.
With going deep into for wound healing theoretical research, once most scholar thought that a dry environment should be provided as far as possible in wound healing process, reduces bacterium infection chance with this, accelerates healing, but in " wet wound healing " [Wound repair and
Regeneration, 2009,17：505；2010,31:83] under theoretical guidance, moistening, FFI wound healing environment has been widely accepted as optimal healing environment, is favourable to continuous process of tissue reparation；Also, whether outstanding dressing is to the performances such as the antibiotic property of wound, gas permeability, moisture retention, absorption sepage ability, and convenience used etc., the healing rate and healing quality of wound are also directly connected to.Therefore new preferable Wound care dressing should also have moisturizing sorptivety, good air permeability, antibacterial bacteriostatic, promotion wound healing, hemostasis and pain-relieving, prevent adhesion, reduce the functions such as scar in addition to the function with traditional dressing.The development of wound dressing and the wound dressing for developing the high-tech constituent content for progressively having developed into today by traditional gauze, the component and species of dressing in recent years in there occurs breakthrough change.The matrix material on the market for dressing has two classes at present：Natural macromolecular material and synthesis high polymer material.Natural macromolecular material mainly has chitosan, hyaluronic acid, collagen, chondroitin sulfate, alginic acid, cellulose etc..Conventional artificial high polymer material includes polyethylene glycol, polyurethane, polyvinylpyrrolidone etc..In general, natural polymer has excellent biocompatibility and degradability, and synthesizing macromolecule has good physical and mechanical properties, processing characteristics and chemical stability.These materials have the advantages of respective and feature, therefore have obtained widely applying with regenerative medicine field in organizational project as biomaterial.
Chitosan is the ideal material of medical dressing, on the one hand they have good biocompatibility, on the other hand there are a variety of reactive active function groups on its strand, the modified methods such as grafting can be easily passed through and be chemically modified, can effectively improve the performance and quality of material.Many researchs have confirmed, chitosan is as unique alkaline polysaccharide in nature, chitosan has excellent gel characteristic, pass through the special electrostatic force of group, hydrophobe effect and hydrogen bond action in chitosan molecule chain, it can be produced with some gelling agents compound, the colloidal materials of large quantity of moisture can be kept by being formed, with very strong moisture absorption, performance of keeping humidity, and permeability is good, enough viscosity can also be provided simultaneously, mucous membrane, skin etc. can be affixed on and do not fallen off, pessimal stimulation and allergic reaction etc. will not be produced to human body skin.And developing the chitosan gel rubber with specific function turns into study hotspot recently, there are many researchs to concentrate at present and develop various chitosans and apply biomedical engineering.
Application publication number CN104630313A（Application number 201510054978.0）Chinese patent literature disclose a kind of preparation method of low-molecular weight chitoglycan dressing, it is to do raw material through lactobacillus-fermented into chitin, again through highly basic acetylation by krill, and with soda acid, solution modeling obtains repeatedly.The invention product has good antibacterial anti hemorrhagic effect.But because preparation process is complicated, environmental pollution is easily caused using a large amount of soda acids, and product is powdered, easily cause during sprinkling it is uneven, without moisture retention, it is impossible to be provided with the moist environment beneficial to wound healing.Application publication number CN102755662 B（Application number 201210260054.2）Chinese patent literature disclose a kind of recombination chitosan gel mould and preparation method thereof, the chitosan of different molecular weight section is used as raw material, it is mixed to prepare a kind of gel mould, there is antibacterial, film forming good air permeability, product of the present invention is in preparation process, inorganic acid or organic acid need to be added, product PH slant acidities is caused, has stimulation to wound；And in order to which film forming adds substantial amounts of glycerine and ethanol, so not only increase cost and cumbersome, and added chemical composition is all different degrees of that human body and environment are impacted.
Thermo-sensitive hydroxyl butyl chitosan protective dressing removes the antibacterial bacteriostatic that has in the present invention, hemostasis and pain-relieving, sorptivety moisturizing, wound healing, mitigate the performances such as patient suffering further, since it is in a liquid state under normal temperature environment shape, at 37 DEG C or so（Body temperature）When quick-gelatinizing characteristic so that can arbitrarily be applied according to wound size, depth, shape when nursing wound, avoid causing dressing and wound suitability bad；And product of the present invention need not add poisonous and hazardous crosslinking agent and a large amount of plasticizer etc. in preparation.Energy-conserving and environment-protective, prepare and use simple and fast.
The content of the invention
Product of the present invention is directed to the deficiency of existing market wound dressing, there is provided one kind has Thermo-sensitive hydroxyl butyl chitosan protective dressing.Products obtained therefrom of the present invention is easy to use, and use range is wide, has good biocompatibility, nontoxic to human body,.With antibacterial bacteriostatic, hemostasis and pain-relieving detumescence, sorptivety moisturizing, promotion wound healing, reduction scar, good air permeability and wound adhesion and other effects.Preparation flow figure is shown in Fig. 1.
It is specific implementation process below：
Step 1：The preparation of Thermo-sensitive hydroxyl butyl chitosan raw material
1st, a certain amount of chitosan crude product is weighed, by 1g:50mL ratio is dissolved in 1% hydrochloric acid, is filtered to remove insoluble matter, and 1mol/L sodium hydroxide solutions are added dropwise to meta-alkalescence, are precipitated, with purifying water washing to neutrality, with ethanol solution desalting and dewatering, the chitosan purified after drying.
2nd, the Chitosan powder of purifying is pressed into 1g：10mL ratio is scattered in basification in 50% sodium hydroxide or potassium hydroxide aqueous solution, and nitrogen protection is lower to heat 24 hours, and temperature is 25 DEG C -80 DEG C.Unnecessary alkali lye is filtered out, remaining solid content is pressed into 1g：20mL ratio is distributed in isopropanol water solution, then adds 1, the 2- epoxy butanes of certain volume, is reacted 1 hour, is heated 24 hours to 72 hours at 25 DEG C -80 DEG C.PH value is adjusted after cooling to neutrality, insoluble matter is filtered out, centrifuges, dries after addition ethanol, obtain with temperature sensitivity hydroxyl butyl chitosan product.
Step 2：The preparation of hydroxyl butyl chitosan protective dressing solution
1st, with liquid：According to production ordering, supplementary material is got, according to the survey report of raw and auxiliary material, its name of an article, lot number, manufacturer and quantity are checked one by one, the calculating of supplementary material, weighs, feed intake and must be checked；After completing above-mentioned steps, a certain amount of hydroxyl butyl chitosan is added in injection water, until being completely dissolved, addition proper amount of active carbon decolourize 20 minutes ~ 60 minutes, opens vavuum pump for stirring, the solution for decolourizing to complete is subjected to coarse filtration, filtrate is collected in the pressure vessel of cleaning, after the completion of to be filtered, good appropriate sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate will be weighed respectively and add and stir to dissolved clarification, start refined filtration, it is standby to collect refined filtration liquid.Pay attention to：Before and after filtering, filter is intended to do bubble point test, should be qualified.
2nd, filling and sealing：Corresponding specification inner packaging material is got according to production ordering, filling and sealing machine and its auxiliary equipment apparatus are cleaned up with water for injection respectively, debugs filling and sealing machine, and corrects loading amount, starts embedding after qualified.Each hour sampling observation loading amount during embedding；Product is hunted leak after the completion of embedding.
Step 3：Sterilizing
The qualified product that will hunt leak is sterilized by regulation packaging：
Sterilizing parameter：Irradiation sterilization electron beam or cobalt -60, irradiation dose are 15KGy ~ 40KGy,；Moist heat sterilization, 116 DEG C ~ 121 DEG C, 15 minutes ~ 40 minutes.
Step 4：Finished product is put in storage
The product completed will be irradiated, after the assay was approved, registration storage.
Product provided by the invention has the following advantages that：
First, hydroxyl butyl chitosan raw material in this product; it is the high-molecular compound chitin purified from shrimp shell; by deacetylated processing; obtain the chitosan of certain deacetylation, then by a kind of hydroxyl butylamino glucose made of hydroxyl butylation and deep processing, there is good antibacterial bacteriostatic, hemostasis and pain-relieving, sorptivety moisturizing, prevent adhesion and tissue repair effect; inflammatory reaction to wound tissue is small; quick-acting haemostatic powder, accelerate wound healing, repair, and there is good biocompatibility.
2nd, product of the present invention has temperature sensitivity, is liquid in normal temperature, and when temperature rises to 37 degrees centigrades（Body temperature）Energy rapid gellation, therefore any plasticizer, crosslinking agent need not be added during preparation, its excitant to human body is greatly reduced, improves the security of product.Also should not other carriers it is compound with support, naturally shaping, have a wide range of application, it is easy to use, different type wound can be met（Such as size, depth and shape）Needs, need to only spray during use, and good with contact site stickiness after its gelation, deformation difficult for drop-off.
Its Thermo-sensitive characteristic is shown in Fig. 2.
3rd, Thermo-sensitive hydroxyl butyl protective dressing, there is good gas permeability, sorptivety moisture retention, splendid moistening microenvironment can be provided to wound repair, promote cell growth, suppress fibroblast, shorten the Wound healing phase, reduce the generation of scar.
4th, hydroxyl butyl chitosan is water soluble chitosan of new generation, compared with other chitosan class products, directly can be configured to the aqueous solution, avoids using acid solution, environment friendliness.
5th, product of the present invention has Thermo-sensitive, because its in body temperature can rapid gellation, so as to be not used crosslinking agent, avoid poisonous and harmful substance from being remained in human body, improve the security of product.
6th, this product can also be used as slow controlled-release material to be mixed with medicine, for the use at position in non-vascular, play slow controlled release drug administration effect.
Brief description of the drawings
Fig. 1 is product preparation method flow chart of the present invention.
Fig. 2 is product temperature sensitivity characteristic figure of the present invention.
Embodiment
Below by specific embodiment and with reference to accompanying drawing, the present invention is described in further detail, but protection domain not limited to this.
Embodiment one
Step 1：The preparation of Thermo-sensitive hydroxyl butyl chitosan raw material
1st, 2000g chitosan crude product is weighed, by 1g:50mL ratio is dissolved in 1% hydrochloric acid, is filtered to remove insoluble matter, and 1mol/L sodium hydroxide solutions are added dropwise to meta-alkalescence, are precipitated, with purifying water washing to neutrality, with ethanol solution desalting and dewatering, the chitosan purified after drying.
2nd, the Chitosan powder 1500g of purifying is pressed into 1g：10mL ratio is scattered in basification in 50% sodium hydroxide or potassium hydroxide aqueous solution, and nitrogen protection is lower to heat 24 hours, and temperature is 40 DEG C.Unnecessary alkali lye is filtered out, remaining solid content is pressed into 1g：20mL ratio is distributed in isopropanol water solution, then adds 1, the 2- epoxy butanes of certain volume, is reacted 1 hour, is heated 36 hours at 50 DEG C.PH value is adjusted after cooling to neutrality, insoluble matter is filtered out, centrifuges, dries after addition ethanol, obtain with temperature sensitivity hydroxyl butyl chitosan product.
Step 2：The preparation of hydroxyl butyl chitosan protective dressing solution
1st, with liquid：According to production ordering, supplementary material is got, according to the survey report of raw and auxiliary material, its name of an article, lot number, manufacturer and quantity are checked one by one, the calculating of supplementary material, weighs, feed intake and must be checked；
Weigh 1000g hydroxyl butyl chitosans to be added in 50Kg injection waters, until being completely dissolved, reduction mixing speed weighs 75g activated carbons and is carefully added into solution for quick stirring, decolourize 40 minutes.After the completion of timing, vavuum pump is opened, the solution for decolourizing to complete is subjected to coarse filtration, filtrate is collected in another clean pressure vessel, wall is cleaned with a little water for injection, after the completion of to be filtered, is respectively added load weighted sodium chloride 400g, disodium hydrogen phosphate 400g and sodium dihydrogen phosphate 75g, and stir to dissolved clarification, compressed air is opened, starts refined filtration, after the completion of filtering, wall is cleaned with a little water for injection, it is standby to merge refined filtration liquid.
Pay attention to：Compressed air need to be through 0.22 micron of filter element filtering；Before and after filtering, filter is intended to do bubble point test, should be qualified.
2nd, filling and sealing：The supplementary material according to needed for production ordering gets inner packaging material etc., filling and sealing machine and its auxiliary equipment apparatus are cleaned up with water for injection respectively, debug filling and sealing machine, and correct loading amount, drain water for injection, pipeline pressure is adjusted to suitable, connects filtrate conduit, 10mL loading amount is set, the appropriate filtrate for starting to get is reclaimed and prepared, again filter, check visible foreign matters situation, start embedding after qualified.Do not have one hour sampling observation loading amount and filtrate clarity each once during embedding；Product is hunted leak after the completion of embedding.
Step 3：Sterilizing
The qualified product that will hunt leak is packed by regulation, send Radiation Center to carry out electron beam sterilization：
Irradiation dose is 25KGy.
Step 4：Finished product is put in storage
The product that irradiation sterilization is completed, after the assay was approved, registration storage.
Embodiment two
Step 1：The preparation of Thermo-sensitive hydroxyl butyl chitosan raw material
1st, 5000g chitosan crude product is weighed, by 1g:50mL ratio is dissolved in 1% hydrochloric acid, is filtered to remove insoluble matter, and 1mol/L sodium hydroxide solutions are added dropwise to meta-alkalescence, are precipitated, with purifying water washing to neutrality, with ethanol solution desalting and dewatering, the chitosan purified after drying.
2nd, the Chitosan powder 4000g of purifying is pressed into 1g：10mL ratio is scattered in basification in 50% sodium hydroxide or potassium hydroxide aqueous solution, and nitrogen protection is lower to heat 24 hours, and temperature is 40 DEG C.Unnecessary alkali lye is filtered out, remaining solid content is pressed into 1g：20mL ratio is distributed in isopropanol water solution, then adds 1, the 2- epoxy butanes of certain volume, is reacted 1 hour, is heated 36 hours at 50 DEG C.PH value is adjusted after cooling to neutrality, insoluble matter is filtered out, centrifuges, dries after addition ethanol, obtain with temperature sensitivity hydroxyl butyl chitosan product.
Step 2：The preparation of hydroxyl butyl chitosan protective dressing solution
1st, with liquid：According to production ordering, supplementary material is got, according to the survey report of raw and auxiliary material, its name of an article, lot number, manufacturer and quantity are checked one by one, the calculating of supplementary material, weighs, feed intake and must be checked；
Weigh 3000g hydroxyl butyl chitosans to be added in 100Kg injection waters, until being completely dissolved, reduction mixing speed weighs 200g activated carbons and is carefully added into solution for quick stirring, decolourize 20 minutes.After the completion of timing, vavuum pump is opened, the solution for decolourizing to complete is subjected to coarse filtration, filtrate is collected in another clean pressure vessel, wall is cleaned with a little water for injection, after the completion of to be filtered, is respectively added load weighted sodium chloride 800g, disodium hydrogen phosphate 800g and sodium dihydrogen phosphate 150g, and stir to dissolved clarification, compressed air is opened, starts refined filtration, after the completion of filtering, wall is cleaned with a little water for injection, it is standby to merge refined filtration liquid.
Pay attention to：Compressed air need to be through 0.22 micron of filter element filtering；Before and after filtering, filter is intended to do bubble point test, should be qualified.
2nd, filling and sealing：The supplementary material according to needed for production ordering gets inner packaging material etc., filling and sealing machine and its auxiliary equipment apparatus are cleaned up with water for injection respectively, debug filling and sealing machine, and correct loading amount, drain water for injection, pipeline pressure is adjusted to suitable, connects filtrate conduit, 50mL loading amount is set, the appropriate filtrate for starting to get is reclaimed and prepared, again filter, check visible foreign matters situation, start embedding after qualified.Do not have one hour sampling observation loading amount and filtrate clarity each once during embedding；Product is hunted leak after the completion of embedding.
Step 3：Sterilizing
The qualified product that will hunt leak is packed by regulation, carries out moist heat sterilization：
Moist heat sterilization parameter：121 DEG C of temperature, 30 minutes time.
Step 4：Finished product is put in storage
By the product completed that sterilizes, after the assay was approved, registration storage.
Embodiment three
Step 1：The preparation of Thermo-sensitive hydroxyl butyl chitosan raw material
1st, 8000g chitosan crude product is weighed, by 1g:50mL ratio is dissolved in 1% hydrochloric acid, is filtered to remove insoluble matter, and 1mol/L sodium hydroxide solutions are added dropwise to meta-alkalescence, are precipitated, with purifying water washing to neutrality, with ethanol solution desalting and dewatering, the chitosan purified after drying.
2nd, the Chitosan powder 7000g of purifying is pressed into 1g：10mL ratio is scattered in basification in 50% sodium hydroxide or potassium hydroxide aqueous solution, and nitrogen protection is lower to heat 24 hours, and temperature is 40 DEG C.Unnecessary alkali lye is filtered out, remaining solid content is pressed into 1g：20mL ratio is distributed in isopropanol water solution, then adds 1, the 2- epoxy butanes of certain volume, is reacted 1 hour, is heated 36 hours at 50 DEG C.PH value is adjusted after cooling to neutrality, insoluble matter is filtered out, centrifuges, dries after addition ethanol, obtain with temperature sensitivity hydroxyl butyl chitosan product.
Step 2：The preparation of hydroxyl butyl chitosan protective dressing solution
1st, with liquid：According to production ordering, supplementary material is got, according to the survey report of raw and auxiliary material, its name of an article, lot number, manufacturer and quantity are checked one by one, the calculating of supplementary material, weighs, feed intake and must be checked；
Weigh 6000g hydroxyl butyl chitosans to be added in 400Kg injection waters, until being completely dissolved, reduction mixing speed weighs 400g activated carbons and is carefully added into solution for quick stirring, decolourize 60 minutes.After the completion of timing, vavuum pump is opened, the solution for decolourizing to complete is subjected to coarse filtration, filtrate is collected in another clean pressure vessel, wall is cleaned with a little water for injection, after the completion of to be filtered, is respectively added load weighted sodium chloride 3200g, disodium hydrogen phosphate 3200g and sodium dihydrogen phosphate 600g, and stir to dissolved clarification, compressed air is opened, starts refined filtration, after the completion of filtering, wall is cleaned with a little water for injection, it is standby to merge refined filtration liquid.
Pay attention to：Compressed air need to be through 0.22 micron of filter element filtering；Before and after filtering, filter is intended to do bubble point test, should be qualified.
2nd, filling and sealing：The supplementary material according to needed for production ordering gets inner packaging material etc., filling and sealing machine and its auxiliary equipment apparatus are cleaned up with water for injection respectively, debug filling and sealing machine, and correct loading amount, drain water for injection, pipeline pressure is adjusted to suitable, connects filtrate conduit, 5mL loading amount is set, the appropriate filtrate for starting to get is reclaimed and prepared, again filter, check visible foreign matters situation, start embedding after qualified.Do not have one hour sampling observation loading amount and filtrate clarity each once during embedding；Product is hunted leak after the completion of embedding.
Step 3：Sterilizing
The qualified product that will hunt leak send Radiation Center to carry out cobalt -60 and sterilized by regulation packaging：
Irradiation dose is 30KGy.
Step 4：Finished product is put in storage
The product that irradiation sterilization is completed, after the assay was approved, registration storage.
Claims (8)
1. a kind of preparation and its application of Thermo-sensitive hydroxyl butyl chitosan protective dressing, it is characterized in that, hydroxyl butyl chitosan is water soluble chitosan, and it has temperature sensitivity, preparation process includes the preparation of hydroxyl butyl chitosan raw material, the preparation of hydroxyl butyl chitosan protective dressing solution, packing, sterilizing, outer packing are made.
2. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 1, it is characterised in that preparing for Thermo-sensitive hydroxyl butyl chitosan protective dressing is as follows：
（1）The preparation of Thermo-sensitive hydroxyl butyl chitosan raw material
A certain amount of chitosan crude product is weighed, by 1g:50mL ratio is dissolved in 1% hydrochloric acid, is filtered to remove insoluble matter, and 1mol/L sodium hydroxide solutions are added dropwise to meta-alkalescence, are precipitated, with purifying water washing to neutrality, with ethanol solution desalting and dewatering, the chitosan purified after drying；
Chitosan powder after purification is pressed into 1g：10mL ratio is scattered in basification in 50% sodium hydroxide or potassium hydroxide aqueous solution, and nitrogen protection is lower to heat 24 hours, and temperature is 25 DEG C -80 DEG C；Unnecessary alkali lye is filtered out, remaining solid content is pressed into 1g：20mL ratio is distributed in isopropanol water solution, then adds 1, the 2- epoxy butanes of certain volume, reaction 1 hour, pH value is adjusted to neutrality after cooling in 24 hours to 72 hours is heated at 25 DEG C -80 DEG C, filters out insoluble matter, centrifuge, dry after addition ethanol, obtain hydroxyl butyl chitosan product；
（2）The preparation of hydroxyl butyl chitosan protective dressing
With liquid：According to production ordering, supplementary material is got, according to the survey report of raw and auxiliary material, its name of an article, lot number, manufacturer and quantity are checked one by one, the calculating of supplementary material, weighs, feed intake and must be checked；After completing above-mentioned steps, a certain amount of hydroxyl butyl chitosan is added in injection water, stirring is until be completely dissolved, add proper amount of active carbon and carry out a period of time of decolourizing, the solution for decolourizing to complete is subjected to coarse filtration, collects filtrate, after the completion of to be filtered, appropriate sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate are added and stirred to dissolved clarification respectively, starts refined filtration, it is standby to collect refined filtration liquid；
Filling and sealing：Filling and sealing machine and its auxiliary equipment apparatus are cleaned up with water for injection respectively, debug filling and sealing machine, and corrects loading amount, starts embedding；Sterilizing just obtains the hydroxyl butyl chitosan protective dressing of the present invention with Thermo-sensitive.
3. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 2, it is characterised in that by weight percentage, it is 0.1 to have the ratio of Thermo-sensitive hydroxyl butyl chitosan and water for injection:100~20：100, the preferable ratio with Thermo-sensitive hydroxyl butyl chitosan and water for injection is 0.5:100~10：100, the ratio of Thermo-sensitive hydroxyl butyl chitosan and water for injection that has for more having choosing is 1:100~3：100.
4. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 2, it is characterised in that by weight percentage, the ratio of sodium chloride and water for injection is 0.8:100, the ratio of disodium hydrogen phosphate and water for injection is 0.8:100, the ratio of sodium dihydrogen phosphate and water for injection is 0.15:100.
5. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 2, it is characterised in that during activated carbon decolorizing, by weight, activated carbon is 0.01 with addition water for injection:100~1:100, the ratio of preferable activated carbon and water for injection is 0.05:100~0.2：100；Bleaching time is 20 minutes ~ 60 minutes.
6. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 2, it is characterised in that sterilization method is：Irradiation sterilization or moist heat sterilization, irradiation sterilization are that electron beam sterilization or cobalt -60 sterilize, and its sterilizing dose is 10kGy ~ 40KGy；Moist heat sterilization parameter：116 DEG C ~ 121 DEG C, 15 minutes ~ 40 minutes.
7. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 1, it is characterised in that product specification is 0.1mL ~ 500mL.
8. the preparation and its application of a kind of Thermo-sensitive hydroxyl butyl chitosan protective dressing according to claim 1, it is characterized in that, product of the present invention is applied to various operative incisions, burn, scald, scratch, ulcer wound surface, bedsore, takes the dermatotome surface of a wound and various surfaces, traumatic trauma surface infestation, the nursing of neonate's pars muscularis etc..
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721689A (en) * | 2018-06-09 | 2018-11-02 | 江苏海尔滋生物科技有限公司 | Biological wound-protecting fluid film and preparation method thereof |
| CN110448722A (en) * | 2019-08-20 | 2019-11-15 | 武汉大学 | A kind of injectable is containing the temperature sensitive composite antibacterial hydrogel material of tannic acid and its preparation and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284884A (en) * | 2008-02-19 | 2008-10-15 | 上海其胜生物材料技术研究所有限公司 | Preparation method of temperature sensitivity chitosan derivate-hydroxybutyl chitosan |
| CN102276756A (en) * | 2011-07-29 | 2011-12-14 | 中国海洋大学 | Preparation method of chitosan hydroxybutyl derivative |
| CN103172762A (en) * | 2011-12-20 | 2013-06-26 | 上海其胜生物制剂有限公司 | Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid |
| WO2015129881A1 (en) * | 2014-02-28 | 2015-09-03 | 国立大学法人大阪大学 | Method for producing three-dimensional cell structure |
| CN105107008A (en) * | 2015-09-16 | 2015-12-02 | 中南民族大学 | Hydroxybutyl chitosan/oxidized sodium alginate/nano-silver composite hydrogel dressing plaster |
| CN204890345U (en) * | 2015-08-07 | 2015-12-23 | 孙汇沣 | Antibiotic biodegradable is medical to apply ointment or plaster |
-
2016
- 2016-06-28 CN CN201610479527.6A patent/CN107537055A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284884A (en) * | 2008-02-19 | 2008-10-15 | 上海其胜生物材料技术研究所有限公司 | Preparation method of temperature sensitivity chitosan derivate-hydroxybutyl chitosan |
| CN102276756A (en) * | 2011-07-29 | 2011-12-14 | 中国海洋大学 | Preparation method of chitosan hydroxybutyl derivative |
| CN103172762A (en) * | 2011-12-20 | 2013-06-26 | 上海其胜生物制剂有限公司 | Preparation method of medical grade temperature sensitive chitosan blocking agent used for preventing leakage of cerebrospinal fluid |
| WO2015129881A1 (en) * | 2014-02-28 | 2015-09-03 | 国立大学法人大阪大学 | Method for producing three-dimensional cell structure |
| CN204890345U (en) * | 2015-08-07 | 2015-12-23 | 孙汇沣 | Antibiotic biodegradable is medical to apply ointment or plaster |
| CN105107008A (en) * | 2015-09-16 | 2015-12-02 | 中南民族大学 | Hydroxybutyl chitosan/oxidized sodium alginate/nano-silver composite hydrogel dressing plaster |
Non-Patent Citations (1)
| Title |
|---|
| CHANG-ZHENG WEI等: "A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions’ prevention", 《BIOMATERIALS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721689A (en) * | 2018-06-09 | 2018-11-02 | 江苏海尔滋生物科技有限公司 | Biological wound-protecting fluid film and preparation method thereof |
| CN110448722A (en) * | 2019-08-20 | 2019-11-15 | 武汉大学 | A kind of injectable is containing the temperature sensitive composite antibacterial hydrogel material of tannic acid and its preparation and application |
| CN110448722B (en) * | 2019-08-20 | 2021-07-06 | 武汉大学 | Temperature-sensitive composite antibacterial hydrogel material capable of being injected and containing tannic acid, and preparation and application thereof |
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