CN107525929B - A kind of preparation method and applications of immunity biosensor - Google Patents
A kind of preparation method and applications of immunity biosensor Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods of immunity biosensor comprising following processing step: 1) preparing reduced graphene modified electrode；2) Prussian blue/graphene modified electrode is prepared；3) by Porcine epidemic diarrhea virus monoclonal antibody drop coating in Prussian blue/graphene modified electrode surface, drying at room temperature, after be immersed in the bovine serum albumen solution that mass concentration is 1~2%, 0.5~1h of water-bath obtains immunity biosensor.Porcine epidemic diarrhea virus monoclonal antibody drop coating in Prussian blue/graphene modified electrode surface and is utilized remaining nonspecific activity site on bovine serum albumen solution enclosed-electrode by the present invention, the important technical parameter in preparation method is limited simultaneously, to make the immunity biosensor being prepared into that there is excellent sensitivity, Stability and veracity.The invention also discloses a kind of applications for quickly detecting the immunity biosensor being prepared by above-mentioned preparation method to pig epidemic diarrhea clinic in non-lab setup.
Description
Technical field
The present invention relates to immunity biosensor field, in particular to the preparation method of a kind of immunity biosensor and answer
With.
Background technique
Graphene is the special two-dimentional carbon nanomaterial of one kind of discovery recent years and only monoatomic thickness, has height
Electric conductivity, big specific surface area and room temperature electron mobility, and have outstanding mechanical mechanics property.Electron velocity under room temperature
It is exceedingly fast, electron mobility is more than 15000cm2/ VS, considerably beyond the transmission speed of electronics in general conductor, and its resistivity
It is extremely low, only 10-8Ω cm, and various atoms and molecule can all be adsorbed by it and desorption, structure is highly stable, graphene modified
Nowadays electrode has been applied in the detection of many kinds of substance.Prussian blue also known as barba hispanica, Berlin blue etc., molecular formula Fe4[Fe
(CN)6]3, there is excellent electrochemical reversibility and excellent catalytic.Its tridimensional network be also it is very unique,
It is to be currently known most unique in inorganic structure, tridimensional network is similar with the organic polymer of crosslinking, has excellent
Rock-steady structure, and its electrochemical reversibility is very high, it can be as the medium of electron transmission, and oxidation-reduction quality can be reduced
Matter, the especially overpotential of peroxide electrochemical reaction, referred to as " artificial peroxidase " are that horseradish enzyme substitutes well
Product, while Prussian blue manufacture is easy, it is low in cost.
Porcine epidemic diarrhea virus causes pig epidemic diarrhea, is a kind of enteric infection of acute high degree in contact of pig
Disease, clinical symptoms are with diarrhea, and it is the one of the major reasons for leading to suckling pig death that dehydration and vomiting, which are main feature, to complete
The pig breeding industry in the world has brought tremendous economic losses.The virus is difficult to separate, and researcher mainly passes through Protocols in Molecular Biology
Such as hybridization in situ technique, round pcr, fluorescent quantitative PCR technique carries out separation identification.Mainly serology is used to examine in clinical detection
It surveys, in Small Volume Serum and tests, immunofluorescence technique, enzyme-linked immunosorbent assay, Immunohistochemical Method and colloidal gold immunochromatographimethod
Method etc..Although these methods can carry out qualitative and quantitative analysis to pig prevalence diarrhea virus, it is taken long time, and detection device
It is inconvenient to carry, it is difficult to accomplish on-site test.
How can sensitive, accurate, stable detection pig prevalence diarrhea by composite graphite alkene and Prussian blue one kind of preparing
The immunity biosensor of virus, makes it can be applied to the detection of non-lab setup, is of great significance in the industry.
Summary of the invention
It is an object of the invention in view of the above shortcomings of the prior art, provide a kind of preparation side of immunity biosensor
Method, at the same provide the immunity biosensor being prepared into non-lab setup to Porcine epidemic diarrhea virus detection
Using.
The technical solution used in the present invention is: a kind of preparation method of immunity biosensor comprising following technique
Step:
1) by graphene oxide dispersion drop coating that concentration is 1mg/mL on electrode matrix surface, drying at room temperature, after by its
Be placed in 0.1mol/L Klorvess Liquid, use cyclic voltammetry with the sweep speed of 100mV/s -1.5~0.5V current potential
20 circle of scanning, obtains reduced graphene modified electrode in range；
2) the resulting reduced graphene modified electrode of step 1) is inserted into the Prussian blue solution that concentration is 0.1mol/L,
It uses cyclic voltammetry to scan 60 circles in 0~1.0V potential range with the sweep speed of 50mV/s, forms Prussian blue deposition
Layer, obtains Prussian blue/graphene modified electrode；
3) by Porcine epidemic diarrhea virus monoclonal antibody drop coating in the resulting Prussian blue/graphene modified electricity of step 2)
Pole surface, drying at room temperature, after be immersed in mass concentration be 1~2% bovine serum albumen solution in, 0.5~1h of water-bath is obtained
Immunity biosensor.
As a further improvement of the foregoing solution, electrode matrix described in step 1) is glass-carbon electrode.
As a further improvement of the foregoing solution, concentration described in step 2) is in the Prussian blue solution of 0.1mol/L
The iron chloride of the potassium ferricyanide, 0.2mol/L containing 0.2mol/L and the potassium chloride of 0.1mol/L.
As a further improvement of the foregoing solution, the drying time of drying at room temperature described in step 3) is 2~4h.
As a further improvement of the foregoing solution, the temperature of water-bath described in step 3) is 37 DEG C.
The present invention also provides a kind of preparation methods as described above to prepare resulting immunity biosensor.
Another technical solution adopted by the present invention is that a kind of immunity biosensor as described above is popular in pig
Application in diarrhea virus detection.
As a further improvement of the foregoing solution comprising following detecting step:
1) immunity biosensor is immersed in the Porcine epidemic diarrhea virus that concentration is 15 μ g/mL, is taken out after 30min
It is eluted with double distilled water, is then immersed in the Porcine epidemic diarrhea virus polyclonal antibody that concentration is 50 μ g/mL, is taken after 30min
It is eluted out with double distilled water, obtains working electrode；
2) working electrode obtained by step 1) is immersed in sample to be tested and is immunoreacted, taken out after reaction slow with PBS
Fliud flushing is cleaned, and using Ag/AgCl electrode as reference electrode, platinum electrode is used as to electrode, using cyclic voltammetry and according to circulation
The qualitative and quantitative detection of voltammogram progress Porcine epidemic diarrhea virus.
As a further improvement of the foregoing solution, used described in step 2) cyclic voltammetry detect determination condition for
It uses the bottom liquid of the pH7.0 through PBS constant volume, sweep speed as 900mV/s, Porcine epidemic diarrhea virus is 10-9~10-5μ g/mL concentration
In a linear relationship in range, detection is limited to 2.5 × 10 when signal-to-noise ratio is 3-9μg/mL。
As a further improvement of the foregoing solution, the bottom liquid is the K containing 0.1mol/L4Fe(CN)6, 0.2mol/L
K3Fe(CN)6The PBS buffer solution that concentration with the KCl of 0.1mol/L is 0.01mol/L.
The beneficial effects of the present invention are:
(1) present invention is by Porcine epidemic diarrhea virus monoclonal antibody drop coating in Prussian blue/graphene modified electrode table
Face simultaneously utilizes remaining nonspecific activity site on bovine serum albumen solution enclosed-electrode, while limiting important in preparation method
Technological parameter, to make the immunity biosensor being prepared into that there is excellent sensitivity, Stability and veracity.
(2) preparation method of the invention is simple and efficient, and the immunity biosensor being prepared into is suitable for non-lab setup
In quick measurement to Porcine epidemic diarrhea virus.
(3) application of the present invention in Porcine epidemic diarrhea virus on-site test has feasibility and high efficiency, basis
Peak point current trend and its amplitude of decline can realize the qualitative and quantitative detection to Porcine epidemic diarrhea virus in cyclic voltammogram.
Detailed description of the invention
Attached drawing 1 of the invention is the cyclic voltammogram of different pH value；
Attached drawing 2 of the invention is the cyclic voltammogram of different bottom liquid；
Attached drawing 3 of the invention is the cyclic voltammogram that difference sweeps speed；
Attached drawing 4 of the invention is the cyclic voltammetric for the Porcine epidemic diarrhea virus that immunity biosensor detects various concentration
Figure；
Attached drawing 5 of the invention is immunity biosensor specific detection result；
Attached drawing 6 of the invention is the cyclic voltammogram that immunity biosensor detection number is a~f sample to be tested.
Specific embodiment
The present invention is specifically described below with reference to embodiment, in order to technical field personnel to of the invention
Understand.It is necessary to it is emphasized that embodiment is only intended to, the present invention will be further described herein, should not be understood as to this
The limitation of invention protection scope, fields person skilled in the art, the non-intrinsically safe that the present invention is made according to foregoing invention content
The modifications and adaptations of property, should still fall within protection scope of the present invention.Mentioned raw materials following simultaneously are unspecified, are
Commercial product；The processing step or preparation method not referred in detail be processing step known to a person skilled in the art or
Preparation method.
Embodiment 1: the preparation of immunity biosensor
A kind of preparation method of immunity biosensor comprising following processing step:
1) take 10 μ L drop coating of graphene oxide dispersion that concentration is 1mg/mL in electrode matrix surface, room temperature with liquid-transfering gun
It is dry, after place it in 0.1mol/L Klorvess Liquid, use cyclic voltammetry with the sweep speed of 100mV/s -1.5~
20 circle of scanning, obtains reduced graphene modified electrode in the potential range of 0.5V；
2) the resulting reduced graphene modified electrode of step 1) is inserted into the Prussian blue solution that concentration is 0.1mol/L,
It uses cyclic voltammetry to scan 60 circles in 0~1.0V potential range with the sweep speed of 50mV/s, forms Prussian blue deposition
Layer, obtains Prussian blue/graphene modified electrode；
3) by Porcine epidemic diarrhea virus monoclonal antibody drop coating in the resulting Prussian blue/graphene modified electricity of step 2)
Pole surface, 2~4h of drying at room temperature, after be immersed in mass concentration be 1~2% bovine serum albumen solution in, in 37 DEG C of temperature strips
0.5~1h of water-bath under part, obtains immunity biosensor.
It is further used as preferred embodiment, electrode matrix described in step 1) is glass-carbon electrode.Specifically, this hair
It is bright before preparing immunity biosensor first to the electrode matrix, i.e. bare glassy carbon electrode is pre-processed: first by glass-carbon electrode
It is polished on chamois leather with the alumina powder that partial size is 0.05 μm, then successively ultrasound is clear in dehydrated alcohol and secondary distilled water
It washes, dries under room temperature.Preprocessed resulting glass-carbon electrode has good surface physicochemical property, advantageously ensures that subsequent
The stability of electrode matrix during electrode modification.
It is further used as preferred embodiment, concentration described in step 1) is the graphene oxide dispersion of 1mg/mL
Be that the dry graphene oxide of 1mg is dispersed in 1mL distilled water, ultrasonic 20min handles to obtain, with good stabilization
Property.
It is further used as preferred embodiment, concentration described in step 2) is in the Prussian blue solution of 0.1mol/L
The iron chloride of the potassium ferricyanide, 0.2mol/L containing 0.2mol/L and the potassium chloride of 0.1mol/L.
Embodiment 2: the determination of immunity biosensor testing conditions
(1) difference pH detects the influence of Porcine epidemic diarrhea virus to it
To be coated with through antibody treated immunity biosensor respectively in pH6.4, pH6.7, pH7.0, pH7.5 and
It is tested in the bottom liquid of pH8.0 by cyclic voltammetry, test results are shown in figure 1, obtains being existed with identical scanning speed
In identical potential range, the bottom liquid of pH7.0 is the optimal conditions that the immunity biosensor detects Porcine epidemic diarrhea virus
One of.
(2) different bottom liquid detect the influence of Porcine epidemic diarrhea virus to it
By through in antibody coating treated immunity biosensor is respectively placed in distilled water and the bottom liquid through PBS constant volume,
It is tested with cyclic voltammetry, test results are shown in figure 2, obtains with identical scanning speed in identical potential range
Interior, the bottom liquid through PBS constant volume is one of the optimal conditions that the immunity biosensor detects Porcine epidemic diarrhea virus.
(3) the different influences swept speed and detect Porcine epidemic diarrhea virus to it
By through antibody coating, treated that immunity biosensor is placed in the bottom liquid through PBS constant volume, it is 50 that speed is swept in control
It is tested within the scope of~990mV/s with cyclic voltammetry, test results are shown in figure 3, and it is whether obvious according to peak change, it obtains
It is one of the optimal conditions that 900mV/s is immunity biosensor detection Porcine epidemic diarrhea virus to speed is swept.
(4) to the determination of Porcine epidemic diarrhea virus sensitivity
By through antibody coating, treated that immunity biosensor is placed in the bottom liquid through PBS constant volume, using cyclic voltammetric
Method tests the Porcine epidemic diarrhea virus sample of various concentration, and test results are shown in figure 4, it is known that with identical scanning
In identical potential range, peak current declines speed as the concentration of Porcine epidemic diarrhea virus increases, this is because anti-
The immune response of antigen-antibody hinders the transmitting of free electron, to obtain immunosensor detection Porcine Epidemic Diarrhea
Poison is 10-9~10-5In a linear relationship in μ g/mL concentration range, related coefficient (r) is 0.9985, detection limit when signal-to-noise ratio is 3
It is 2.5 × 10-9μg/mL。
Embodiment 3: the performance detection of immunity biosensor
(1) stability
The immunosensor is put in 4 DEG C of refrigerators and is saved, is detected again again after placing 30 days, and record current responds,
Compared with initial signal, with good consistency.
(2) specific
Control sample containing PRRVS, CSFV, FMDV positive is detected using the immunosensor, is detected with cyclic voltammetry
Virus in sample, testing result is as shown in figure 5, the electric current decline of visual contrast sample obviously, is clearly distinguishable to pig
The testing result of epidemic diarrhea virus.
Embodiment 4: application of the immunity biosensor in Porcine epidemic diarrhea virus detects
Immunity biosensor includes the following steps: the detection of Porcine epidemic diarrhea virus in the present invention
1) it takes the intestines of sick pig, excrement tissue to be fully ground respectively and is diluted dissolution with the PBS of the pH7.4 after sterilizing,
High speed centrifugation handles 10min under 5000r/min speed conditions after multigelation 3 times, and repeated centrifugation is handled 3 times, takes supernatant
Loaded in sterile centrifugation tube, call number is the sample to be tested of a~f, and is put in 4 DEG C of refrigerators and is sealed；
2) immunity biosensor is immersed in the Porcine epidemic diarrhea virus that concentration is 15 μ g/mL, is taken out after 30min
It is eluted with double distilled water, is then immersed in the Porcine epidemic diarrhea virus polyclonal antibody that concentration is 50 μ g/mL, is taken after 30min
It is eluted out with double distilled water, obtains working electrode；
3) working electrode obtained by step 2) respectively detects the sample to be tested that step 1) institute's call number is a~f, has
Body is to be immersed in step 2) in each sample to be tested to be immunoreacted, and taking-up is cleaned with PBS buffer solution after reaction, with Ag/
As reference electrode, platinum electrode is used as to electrode AgCl electrode, and cyclic voltammetry is used under the testing conditions that embodiment 3 determines
And the qualitative and quantitative detection of Porcine epidemic diarrhea virus is carried out according to cyclic voltammogram, such as to the testing result of each sample to be tested
Shown in Fig. 6.
Bottom liquid described in above-described embodiment is the K containing 0.1mol/L4Fe(CN)6, 0.2mol/L K3Fe(CN)6With
The concentration of the KCl of 0.1mol/L is the PBS buffer solution of 0.01mol/L.
In this embodiment of the invention, it can be seen that peak point current declines according to Fig. 6 testing result, that is, be fixed on electrode surface
On antibody and sample to be tested in virus immune, loudness current reduction, in this, as the immunity biosensor pair occurs
The foundation of Porcine epidemic diarrhea virus qualitative detection, while to be immunoreacted the amplitude of front and back peak point current decline in test result
As the immunity biosensor to the foundation of Porcine epidemic diarrhea virus quantitative detection.
Above-described embodiment is the preferred embodiment of the present invention, all with similar technique of the invention and made equivalence changes,
It should belong to protection category of the invention.
Claims (6)
1. a kind of preparation method of immunity biosensor, which is characterized in that comprise the technical steps that:
1) by graphene oxide dispersion drop coating that concentration is 1mg/mL on electrode matrix surface, drying at room temperature, after place it in
In 0.1mol/L Klorvess Liquid, use cyclic voltammetry with the sweep speed of 100mV/s -1.5~0.5 V potential range
Interior 20 circle of scanning, obtains reduced graphene modified electrode；
It 2) is to be used in the Prussian blue solution of 0.1mol/L by the resulting reduced graphene modified electrode insertion concentration of step 1)
Cyclic voltammetry scans 60 circles with the sweep speed of 50mV/s in 0~1.0V potential range, forms Prussian blue sedimentary, obtains
Prussian blue/graphene modified electrode；
3) by Porcine epidemic diarrhea virus monoclonal antibody drop coating in the resulting Prussian blue/graphene modified electrode table of step 2)
Face, drying at room temperature, after be immersed in mass concentration be 1~2% bovine serum albumen solution in, 0.5~1h of water-bath is obtained immune
Biosensor.
2. a kind of preparation method of immunity biosensor according to claim 1, it is characterised in that: described in step 1)
Electrode matrix be glass-carbon electrode.
3. the preparation method of resulting a kind of immunity biosensor according to claim 1, it is characterised in that: described in step 2)
Concentration be 0.1mol/L Prussian blue solution in the potassium ferricyanide containing 0.2mol/L, 0.2mol/L iron chloride and
The potassium chloride of 0.1mol/L.
4. the preparation method of resulting a kind of immunity biosensor according to claim 1, it is characterised in that: described in step 3)
Drying at room temperature drying time be 2~4h.
5. the preparation method of resulting a kind of immunity biosensor according to claim 1, it is characterised in that: described in step 3)
Water-bath temperature be 37 DEG C.
6. a kind of preparation method as claimed in any one of claims 1 to 5 prepares resulting immunity biosensor.
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| CN110161236A (en) * | 2018-01-18 | 2019-08-23 | 广西壮族自治区兽医研究所 | It is a kind of detect bovine viral diarrhea virus electrochemical immunosensor and its application |
| CN109444240B (en) * | 2018-11-06 | 2021-01-26 | 湖北师范大学 | Prussian blue-based electrochemical immunosensor, electrochemical immunosensing method established based on sensor and application |
| CN111751546B (en) * | 2019-03-29 | 2023-11-10 | 中国科学院金属研究所 | Preparation method and application of calprotectin biosensor based on graphene |
| WO2021081424A1 (en) * | 2019-10-25 | 2021-04-29 | University Of Utah Research Foundation | Micro-balance biosensors to detect whole viruses |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101980018A (en) * | 2010-10-29 | 2011-02-23 | 上海交通大学 | Method for preparing immuno biosensor for measuring ractopamine (RAC) |
| CN103506161A (en) * | 2013-09-22 | 2014-01-15 | 青岛大学 | Graphene composite, application of graphene composite to catalytic methanol oxidation, chemically modified electrode and preparation method of graphene composite |
| CN103777010A (en) * | 2014-01-27 | 2014-05-07 | 深圳市宝舜泰生物医药股份有限公司 | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for porcine epidemic diarrhea viruses and preparation method thereof |
| CN104862285A (en) * | 2015-05-12 | 2015-08-26 | 华中农业大学 | Porcine epidemic diarrhea virus antibody capture based ELISA detection method and application |
-
2017
- 2017-08-11 CN CN201710684504.3A patent/CN107525929B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101980018A (en) * | 2010-10-29 | 2011-02-23 | 上海交通大学 | Method for preparing immuno biosensor for measuring ractopamine (RAC) |
| CN103506161A (en) * | 2013-09-22 | 2014-01-15 | 青岛大学 | Graphene composite, application of graphene composite to catalytic methanol oxidation, chemically modified electrode and preparation method of graphene composite |
| CN103777010A (en) * | 2014-01-27 | 2014-05-07 | 深圳市宝舜泰生物医药股份有限公司 | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for porcine epidemic diarrhea viruses and preparation method thereof |
| CN104862285A (en) * | 2015-05-12 | 2015-08-26 | 华中农业大学 | Porcine epidemic diarrhea virus antibody capture based ELISA detection method and application |
Non-Patent Citations (1)
| Title |
|---|
| 电化学制备普鲁士蓝-石墨烯复合物及对过氧化氢电催化性能研究;陈体伟 等;《分析试验室》;20150531;第34卷(第5期);第587-590页 * |
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