CN107525918A - A kind of Sudan red chemiluminescence detection kit and preparation method thereof - Google Patents
A kind of Sudan red chemiluminescence detection kit and preparation method thereof Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
- G01N2446/80—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids
- G01N2446/90—Magnetic particle immunoreagent carriers characterised by the agent used to coat the magnetic particles, e.g. lipids characterised by small molecule linker used to couple immunoreagents to magnetic particles
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Abstract
The invention discloses a kind of Sudan red chemiluminescence detection kit and preparation method thereof.The kit includes：Acridinium ester label, coupling have magnetic particle, calibration object solution, cleaning fluid, chemiluminescence preexciting liquid A, the chemiluminescence exciting liquid B of antigen or antibody.Kit of the present invention is using Magneto separate chemiluminescence as detection means, in combination with acridinium ester label technology.Kit of the present invention is simple to operate, and reaction condition is gentle, and light value stabilization, is influenceed by external condition few；Compared with prior art, there is high sensitivity, detection is quick and convenient, accuracy is high, reproducible, high specificity.
Description
Technical field
The invention belongs to technical field of immune assay, is related to food safety detection technology, and in particular to a kind of the Sudan
Red I chemiluminescence detection kit and preparation method thereof.
Background technology
Tonyred (Sudan) is a kind of artificial synthesized lipophilicity azo dyes, mainly including Sudan red, II, III and
IV 4 types, frequently as a kind of industrial dye, it is mainly used in the dyeing of machine oil, wax and shoe polish etc..In food processing process, Soviet Union
Pellet is red, to increase the color and luster of food, to promote the appetite of people because its vivid red is often added in food by illegal businessman, its
In with Sudan red most commonly seen.Contain a kind of compound for being naphthalene in Sudan red chemical composition, the material has azo knot
Structure, because the property of this chemical constitution determines that it has carcinogenicity.Research shows that Sudan red dyes can cause muroid to be suffered from
Cancer, its metabolite aniline can be done directly on human hepatocyte, and so as to cause toxic hepatic disease, liver is that Sudan red 1 produces
The first target organs of carcinogenicity, it can additionally cause the tumour of the internal organs such as bladder, spleen.Long-term intake aniline can cause human body
Nervous system damage, international cancer research institution are classified as three class carcinogenic substances.Nineteen ninety-five, European Union forbade using tonyred as food
Added with pigment in food, China《Food additives use sanitary standard》(GB2760-1996) also not in food
Add tonyred.Therefore, efficient detection is carried out to tonyred for ensureing that it is particularly significant that the safety of food especially dairy products has
Meaning.
Method currently used for detecting Sudan red mainly has high performance liquid chromatography, gas chromatography-mass spectrometry, liquid
Phase chromatography-mass spectroscopy/mass spectrography, colloidal gold method, enzyme-linked immunosorbent assay, chemiluminescence immunoassay etc..Wherein, efficient liquid phase
Chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrography/mass spectrography need large-scale precision instrument, cumbersome time-consuming, detection
High cost, Finite Samples are detected, are not suitable with the detection of high-volume sample；CN 101261271 A（2008.9）Disclose one kind
Tonyred immune chromatography test paper detecting method, this method are marked using collaurum, and colloid gold label detection is with naked eyes
Visible color is characterized, and error is larger, and cumbersome, and flow is more, is more easy to error occur, the shortcomings such as sensitivity is low；
CN 11844926 A（2006.10）A kind of enzyme linked immunological kit and method for detecting tonyred is disclosed, the kit uses
Enzyme-linked immunoassay method is detected, and enzyme-linked immunosorbent assay has sensitivity is low, be not easy realization full-automation, detection time is grown etc.
The shortcomings that；CN 202057602 U（2011.11）A kind of reagent box for luminescent enzyme-linked immunoassay of tonyred is disclosed, the kit
Using horseradish peroxidase as label, the shortcomings that being marked using horseradish peroxidase is that enzymatic is affected by environment
Greatly：As thimerosal, acid-base value, ion can all impact to it, stability is weaker, so as to influence measurement result.
The present invention is detected using Magneto separate chemiluminescence as detection means in combination with acridinium ester label technology.
Acridine substituent is used for immunoassay tool as chemiluminescent labels and had many advantages, and mainly has：1. chemistry
Reaction is simple, it is quick, without catalyst, having H2O2Dilute alkaline solution in can light；2. background luminescence is low, signal to noise ratio is high,
Luminescence-producing reaction disturbing factor is few；3. emission type lights for flash type, the quick concentration of light release, luminous efficiency are high, luminous intensity
Greatly；4. acridinium ester molecular weight is small, avoids covering antibody combining site, system overall sensitivity can be improved；5. it is easy to and protein
Photon yield is not reduced after being coupled and being coupled；6. label is stable, is not influenceed by ambient oxidant, can be preserved at 2-8 DEG C
Several months long.Therefore acridine substituent is a kind of very effective chemiluminescent labels.
Magneto separate immunoassay technology is a kind of a kind of novel immune established using magnetic particle as solid phase carrier of separating
Detection method, this method can be such that the association reaction of antigen, antibody is carried out under conditions of approximate liquid phase, thus rapid reaction, thorough
Bottom.
The content of the invention
The technical problems to be solved by the invention are overcome the deficiencies in the prior art, there is provided a kind of easy to operate, sensitivity
Higher, rapid reaction, Sudan red magnetic microparticle chemiluminescence measure kit is quantitative determined stably, exactly.
To achieve the above object, the present invention can take following technical proposals：
A kind of Sudan red chemical luminescence reagent kit and its composition of the present invention, including following components：Acridinium ester label
Antigen or antibody, coupling have the magnetic particle of antigen or antibody, calibration object solution, cleaning fluid, chemiluminescence preexciting liquid A and change
Learn luminous exciting liquid B.
Described chemiluminescent labels are acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS；Wherein, acridinium ester
The antigen of mark or the ratio of antibody are 5-100:1.
Described acridinium ester label buffer solution is that pH 8.0-11.0, concentration are 0.05-0.50 mol/L Na2CO3-
NaHCO3Buffer solution.
Described magnetic particle directly can be coupled with Sudan red antibody or antigen, or magnetic particle and Streptavidin are coupled,
Use the Sudan red antibody of biotin labeling or antigen simultaneously.
Described calibration object is by the calibration object solution composition of 6 various concentrations, and calibration object solution is specifically by Sudan red antigen
Formed with calibration object buffer solution.
Sudan red 6 described different calibration object concentration gradients are 0 ng/mL, 0.05 ng/mL, 0.5 ng/mL, 5
ng/mL、50 ng/mL、100 ng/mL。
Described calibration object buffer solution is the Tris-HCl bufferings containing 0.5-5.0% BSA and 0.1-0.5% PC300
Liquid.
Described chemiluminescence preexciting liquid A is by H2O2And HNO3Mixed liquor composition.Wherein, H2O2Mass fraction be
0.05-5%, HNO3Concentration is 0.05-2.5 mol/L；Chemiluminescence exciting liquid B by Triton X-100 and NaOH mixed liquor
Composition.Wherein, Triton X-100 concentration is 0.05-2.0 mol/L, and NaOH concentration is 0.05-1.0 mol/L.
The Tris-HCl solution that described cleaning fluid is pH 7.0-9.0, concentration is 5-50 mmol/L, wherein being containing concentration
0.05-0.50 mol/L NaCl and 0.01-0.25% Tween-20.
The principle that detection kit of the present invention uses is competition law, particular by anti-in the antigen and system in sample
Former competition binding specific antibody, using acridinium ester react caused by photon to detect production concentration.
The present invention has advantages below：
1. kit of the present invention is carried out using Magneto separate chemiluminescence as detection means using acridinium ester label technology
Detect to establish a kind of Sudan red direct chemical luminescence reagent kit of detection.Acridinium ester have molecular weight is small, background luminescence is low,
Signal to noise ratio is high, luminous efficiency is high, mark is stable, can improve the clear superiority of system overall sensitivity.
2. the present invention requires low to Sample pretreatment, sample can be used to detect after the processing such as extraction, dilution, and can
Realize high-volume automatic detection.
To sum up, kit of the present invention is simple to operate, has luminous value stabilization, high sensitivity, detection quick and convenient, accurate
The advantages that true property is high, reproducible, high specificity.
Embodiment
The present invention provides a kind of Sudan red chemical luminescence reagent kit and its composition, to make the purpose of the present invention, technical side
Case and effect definitely, it is clear, the present invention is described in more detail below.
The present invention provides a kind of Sudan red chemical luminescence reagent kit, and the kit includes：Acridinium ester label, coupling
There are magnetic particle, calibration object solution, cleaning fluid, chemiluminescence preexciting liquid A, the chemiluminescence exciting liquid B of antigen or antibody.
Kit of the present invention is entered using Magneto separate chemiluminescence as detection means in combination with acridinium ester label technology
Row detection.
Embodiment 1：The establishment and its preparation of kit 1
1. kit 1 is set up
A kind of Sudan red chemiluminescence detection kit is set up, it is contained following component：
The Sudan red antibody of acridinium ester label；
The Sudan red antigen of carboxyl magnetic bead coupling；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Sudan red series of calibration product solution, concentration are respectively：0 ng/mL、0.05 ng/mL、0.5 ng/mL、5 ng/mL、50
ng/mL、100 ng/mL；
Cleaning fluid, specially pH 7.2, the Tris-HCl solution that concentration is 25 mmol/L, wherein being 0.15 mol/L containing concentration
NaCl and 0.05% Tween-20.
2. coupling has the preparation of the magnetic particle suspension of Sudan red antigen
（1）1 mg carboxyls magnetic particle is taken in 0.5 mL centrifuge tubes, adds 200 μ L 0.1 mol/L MES（pH 5.0）Buffering
Liquid, it is vortexed and mixes, be placed on magnetic frame, standing 5 min makes magnetic particle be separated with liquid, supernatant discarding, washs 3 times, adds
200 μ L MES buffer solutions（pH 5.0）, it is vortexed.
（2）Add 20 μ L（20 μg）Sudan red antigen, be vortexed, on rotatable reactor, be incubated at room temperature 30 minutes.
（3）Add the coupling reagent EDC that 10 μ L concentration are 10 mg/mL（1- (3- dimethylamino-propyls) -3- ethyl carbon
Diimine）, it is vortexed, on rotatable reactor, is incubated at room temperature 2 hours.
（4）Supernatant is removed, adds 200 μ L cleaning buffer solutions（TBS+0.05% Tween-20）, wash 3 times.
（5）Closed with containing 1% BSA buffer solutions, repeatedly closing 4 times, every time 10 min.The magnetic particle suspension is put
In 2-8 DEG C of preservation.
3. the preparation of the Sudan red antibody of acridinium ester label
（1）A certain amount of Sudan red antibody is placed in bag filter, and bag filter is placed in the mark buffer solution not less than 1 L
Middle dialysis, during which buffer solution at least change 3 times, last time dialysed overnight, mark buffer solution be pH 10.1, concentration 0.1
Mol/L Na2CO3-NaHCO3Buffer solution.
（2）A certain amount of acridinium ester is weighed, is NSP-DMAE-NHS from chemiluminescent labels, is dissolved in anhydrous dimethyl base
In formamide DMF, the acridine ester solution that concentration is 6.5 mmol/L is made into.
（3）Sudan red antibody after dialysis is placed in a centrifuge tube, adds 6.5 mmol/L's of certain volume
The molar ratio of NSP-DMAE-NHS DMF solutions, acridinium ester and antibody is 10:1,200 μ L mark buffer solutions are added, at room temperature
React 1 h.The μ L of 10 g/L lysines 100 are added, continues to react 15 min, makes mark reaction terminating.
（4）Label NSP-DMAE-NHS-Ab and free NSP-DMAE-NHS passes through Sephadex G-50 posts（1×25
cm）Separation, with the mol/L of purification buffer 0.1 PBS（pH 6.3）Balance and elute chromatographic column.Chromatograph is used in separation process
Protein peak is detected, measures the chemiluminescence intensity and 280 nm absorbances of efflux respectively.Collect shading value height and absorbance
Big eluent, add 1% BSA（Volume）After dispense stored frozen.
4. the preparation of Sudan red calibration object
It is 0 to be configured to indicate concentration by Sudan red sterling with the Tris-NaCl buffer solutions containing 2% BSA and 0.25% PC300
Ng/mL, 0.05 ng/mL, 0.5 ng/mL, 5 ng/mL, 50 ng/mL, a series of 100 ng/mL calibration objects.
5. the preparation of chemiluminescence exciting agent
（1）Chemiluminescence preexciting liquid A is by H2O2And HNO3Mixed liquor composition.Wherein, H2O2Mass fraction be 1.5%,
HNO3Concentration is 0.1 mol/L, is distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
（2）Chemiluminescence exciting liquid B is made up of Triton X-100 and NaOH mixed liquor.Wherein, Triton X-100
Concentration is 0.1 mol/L, and NaOH concentration is 0.35 mol/L, is distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
Embodiment 2：The establishment and its preparation of kit 2
1. the establishment of kit 2
A kind of Sudan red chemiluminescence detection kit is set up, it is contained following component：
The Sudan red antigen of acridinium ester label；
Carboxyl magnetic bead is coupled Sudan red antibody；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Sudan red series of calibration product solution, concentration are respectively：0 ng/mL、0.05 ng/mL、0.5 ng/mL、5 ng/mL、50
ng/mL、100 ng/mL；
Cleaning fluid, specially pH 7.2, the Tris-HCl solution that concentration is 25 mmol/L, wherein being 0.15 mol/L containing concentration
NaCl and 0.05% Tween-20.
2. coupling has the preparation of the magnetic particle suspension of Sudan red antibody
（1）1 mg carboxyls magnetic particle is taken in 0.5 mL centrifuge tubes, adds 200 μ L 0.1 mol/L MES（pH 6.0）Buffering
Liquid, it is vortexed and mixes, be placed on magnetic frame, standing 5 min makes magnetic particle be separated with liquid, supernatant discarding, washs 3 times, adds
200 μ L MES（pH 6.0）Buffer solution, it is vortexed.
（2）Add 15 μ L（15 μg）Sudan red antibody, it is vortexed, on rotatable reactor, is incubated at room temperature 30 minutes.
（3）Add the coupling reagent EDC that 10 μ L concentration are 10 mg/mL（1- (3- dimethylamino-propyls) -3- ethyl carbon
Diimine）, it is vortexed, on rotatable reactor, is incubated at room temperature 2 hours.
（4）Supernatant is removed, adds 200 μ L cleaning buffer solutions（TBS+0.05% Tween-20）, wash 3 times.
（5）Closed with containing 1% BSA buffer solutions, repeatedly closing 4 times, every time 10 min.The magnetic particle suspension is put
In 2-8 DEG C of preservation.
3. the preparation of the Sudan red antigen of acridinium ester label
（1）A certain amount of Sudan red antigen is placed in bag filter, and bag filter is placed in the mark buffer solution not less than 1 L
Middle dialysed overnight, the Na that mark buffer solution is pH 9.8, concentration is 0.1 mol/L2CO3-NaHCO3Buffer solution.
（2）A certain amount of acridinium ester is weighed, is NSP-DMAE-NHS from chemiluminescent labels, is dissolved in anhydrous dimethyl base
In formamide DMF, the acridine ester solution that concentration is 6.5 mmol/L is made into.
（3）Sudan red antigen after dialysis is placed in a centrifuge tube, adds 6.5 mmol/L's of certain volume
The molar ratio of NSP-DMAE-NHS DMF solutions, acridinium ester and antigen is 20:1,200 μ L mark buffer solutions are added, at room temperature
React 1 h.Add the μ L of 10 g/L lysines 100 to continue to react 15 min, make mark reaction terminating.
（4）Label NSP-DMAE-NHS-Ag and free NSP-DMAE-NHS passes through Sephadex G-50 posts（1×25
cm）Separation, with the mol/L of purification buffer 0.1 PBS（pH 6.3）Balance and elute chromatographic column.Chromatograph is used in separation process
Protein peak is detected, measures the chemiluminescence intensity and 280 nm absorbances of efflux respectively.Collect shading value height and absorbance
Big eluent, add 1% BSA（Volume）After dispense stored frozen.
4. the preparation of Sudan red calibration object
It is 0 to be configured to indicate concentration by Sudan red sterling with the Tris-HCl buffer solutions containing 2% BSA and 0.25% PC300
Ng/mL, 0.05 ng/mL, 0.5 ng/mL, 5 ng/mL, 50 ng/mL, a series of 100 ng/mL calibration objects.
5. the preparation of chemiluminescence exciting agent：
（1）Chemiluminescence preexciting liquid A is by H2O2And HNO3Mixed liquor composition.Wherein, H2O2 Mass fraction be 1.5%,
HNO3Concentration is 0.1 mol/L, is distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
（2）Chemiluminescence exciting liquid B is made up of Triton X-100 and NaOH mixed liquor.Wherein, Triton X-100
Concentration is 0.1 mol/L, and NaOH concentration is 0.35 mol/L, is distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
Embodiment 3：Sudan red detection in actual sample
The use operation sequence of the Sudan red immue quantitative detection reagent box of the present invention is as follows：
1. Sample pretreatment
Weigh 1g testing samples（Such as thick broad-bean sauce, thick chilli sauce, chilli powder）In 10 mL centrifuge tubes；Add the mol/L of 1 mL 2
Sodium hydroxide solution and 5 mL acetonitriles as extract solution, the min of vortex oscillation 10；4000 r/min centrifuge 10 min, take
Clearly；Add the mol/L of 2 mL 2 sodium hydroxide solution and 2 mL n-hexanes as extract solution, the min of vortex oscillation 4；4000 r/
Min centrifuges 10 min, takes supernatant；Add sample diluting liquid and press 1:20 volume ratio is diluted；The solution after dilution is taken to carry out
Detection.
（2）Sample diluting liquid is the phosphate buffer that pH values are 7.2, wherein containing 10% methanol, 0.1% TritonX-
100。
2. the detection of kit
（1）By μ L of sample to be tested 100, μ L of coupling magnetic particle suspension 150, the μ L of acridinium ester label 150, sequentially add anti-
Ying Guanzhong, vibration mix, 37 DEG C of 15 min of incubation.
（2）Separation, clean 5 times.
（3）Reaction vessel after washing, which is fully vibrated, makes magnetic particle scatter.
（4）100 μ L chemiluminescence exciting liquid B are added after adding 100 μ L chemiluminescence preexciting liquid A, 1 s, measure it
Relative luminous intensity, Sudan red content luminous intensity proportion relation corresponding thereto in sample.
Embodiment 4：Performance indications testing result
（1）The sensitivity of kit
20 retests are carried out to zero standard solution, the average value for taking zero standard solution to determine adds 3 times of standard deviation, i.e.,
For the sensitivity of this kit.This kit is 0.05 ng/mL to Sudan red sensitivity.
（2）The specificity of kit
The competition medicine similar to Sudan red structure or function：Indigo, carmine, amaranth, lemon yellow.By kit step
Operation, Sudan red, indigo, carmine, amaranth, lemon yellow are separately added into, make suppression curve, calculated according to linear equation
50% inhibition concentration of each competitor.Cross reacting rate is antibody to Sudan red IC50With antibody to Sudan red competitor
IC50The ratio between percentage.As a result show：This kit has higher specificity to Sudan red, pair with Sudan red structure or
The intimate competition equal no cross reaction of medicine of person.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of Sudan red chemiluminescence detection kit and its composition, it is characterised in that including：The Soviet Union of acridinium ester label
Red red I antibody, coupling have magnetic particle, calibration object solution, cleaning fluid, chemiluminescence preexciting liquid A, the chemistry of Sudan red antigen
Luminous exciting liquid B.
A kind of 2. Sudan red chemiluminescence detection kit according to claim 1, it is characterised in that described change
Luminous marker is acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS.
A kind of 3. Sudan red chemiluminescence detection kit according to claim 1, it is characterised in that described a word used for translation
Pyridine ester mark is Sudan red antibody.
A kind of 4. Sudan red chemiluminescence detection kit according to claim 1, it is characterised in that described magnetic
Particulate directly can be coupled with Sudan red antigen, or magnetic particle and Streptavidin are coupled, while using biotin labeling the Sudan
Red I antigen.
A kind of 5. Sudan red chemiluminescence detection kit according to claim 1, it is characterised in that described school
Quasi- product are using the Tris-HCl buffer solutions containing 0.5-5.0% BSA and 0.1-0.5% PC300 as matrix, are added Sudan red pure
The calibration object solution for the series concentration gradient that product configuration forms.
A kind of 6. Sudan red chemiluminescence detection kit according to claim 1, it is characterised in that described change
Luminous preexciting liquid A is learned by H2O2And HNO3Mixed liquor composition, chemiluminescence exciting liquid B is by Triton X-100 and NaOH
Mixed liquor forms.
7. a kind of Sudan red chemiluminescence detection kit and its composition, it is characterised in that including：The Soviet Union of acridinium ester label
Red red I antigen, coupling have magnetic particle, calibration object solution, cleaning fluid, chemiluminescence preexciting liquid A, the chemistry of Sudan red antibody
Luminous exciting liquid B.
A kind of 8. Sudan red chemiluminescence detection kit according to claim 7, it is characterised in that described change
Luminous marker is acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS.
A kind of 9. Sudan red chemiluminescence detection kit according to claim 7, it is characterised in that described a word used for translation
Pyridine ester mark is Sudan red antigen.
A kind of 10. Sudan red chemiluminescence detection kit according to claim 7, it is characterised in that described magnetic
Magnetic particle and Streptavidin or can be coupled by particulate directly with Sudan red antibody coupling, while using biotin labeling the Sudan
Red I antibody.
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| CN102115451A (en) * | 2010-12-22 | 2011-07-06 | 南京大学 | Sudan 1 capable of being directly marked and preparation method and application thereof |
| CN102928406A (en) * | 2011-08-09 | 2013-02-13 | 北京勤邦生物技术有限公司 | Magnetic particle chemiluminescence kit for detecting sudan I, and applications thereof |
| CN102565405A (en) * | 2011-08-24 | 2012-07-11 | 苏州长光华医生物试剂有限公司 | Method for immunological detection by combining acridinium ester labeling technology with general magnetic particles |
| CN104849469A (en) * | 2015-04-16 | 2015-08-19 | 广州市达瑞生物技术股份有限公司 | Kit for detecting NGAL content and preparation method thereof |
| CN105759061A (en) * | 2016-02-26 | 2016-07-13 | 厦门大学附属中山医院 | Human serum adiponectin detection kit based on micro-particle chemiluminescence immune assay |
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