CN107517931A - A kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and softening decolorising agent - Google Patents
A kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and softening decolorising agent Download PDFInfo
- Publication number
- CN107517931A CN107517931A CN201710753975.5A CN201710753975A CN107517931A CN 107517931 A CN107517931 A CN 107517931A CN 201710753975 A CN201710753975 A CN 201710753975A CN 107517931 A CN107517931 A CN 107517931A
- Authority
- CN
- China
- Prior art keywords
- softening
- bat moth
- chorion
- host
- cordyceps sinensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001136 chorion Anatomy 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 12
- 238000002845 discoloration Methods 0.000 title abstract description 6
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 26
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 26
- 210000004681 ovum Anatomy 0.000 claims abstract description 26
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 239000004155 Chlorine dioxide Substances 0.000 claims abstract description 10
- 235000019398 chlorine dioxide Nutrition 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 15
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000003643 water by type Substances 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 abstract description 9
- 238000011160 research Methods 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 3
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 241000255789 Bombyx mori Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- NHYCGSASNAIGLD-UHFFFAOYSA-N Chlorine monoxide Chemical compound Cl[O] NHYCGSASNAIGLD-UHFFFAOYSA-N 0.000 description 2
- 239000004902 Softening Agent Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OEYAMMOBGOPKMU-UHFFFAOYSA-N CCO.O=[Cl]=O Chemical compound CCO.O=[Cl]=O OEYAMMOBGOPKMU-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000221760 Claviceps Species 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000238367 Mya arenaria Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to biological technical field, there is provided a kind of host of Cordyceps sinensis bat moth chorion softens discoloration method, is handled 10 to 60 minutes it is characterized in that bat moth ovum is immersed in the solution of 0.1 0.2% chlorine dioxide.Present invention also offers a kind of host of Cordyceps sinensis bat moth chorion to soften decolorising agent.This method is simple to operate, controllable, and its softening decolorising agent composition is cheap, green, have updated the existing technology for softening decolouring field for chorion completely, and the softening particularly to bat moth chorion is decolourized, for bat moth ovum, further research provides possibility.
Description
Technical field
The invention belongs to biological technical field, it is related to a kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and soft
Change decolorising agent.
Background technology
Cordyceps sinensis, it is rare traditional Chinese medicine traditional in Chinese history, is by the cordyceps sinensis of Clavicipitaceae Claviceps
Bacterium parasitizes the bat moth larvae in alpine meadow soil, larva body is ossify, and under optimum conditions, by bombys batryticatus head end pumping
Go out long bar-shaped stroma and formed, i.e., the complex that the fructification of aweto is formed with bombys batryticatus sclerotium (larva corpse).
Host of the bat moth larvae as aweto, studies it of crucial importance.And the research of embryonic development for
It is the basic of life for eucaryote, chorion treatment technology is development research, the exploitation of ovum Cryopreservation Technology for carrying out bat moth ovum
With the premise of technique for gene engineering operation.The chorion of bat moth it is pitch-black it is shinny, quality is hard, be to resist adverse environment protection embryo
The important barrier of development, it yet there are no on the resolution of bat moth ovum chorion, soften, the report of decolouring technology.Therefore, bat moth
The processing of chorion is the first step of this kind of research.
The content of the invention
In terms of chorion softening technology of the prior art is silkworm seed mostly, the softening technology of silkworm seed to bat moth ovum entirely without
Effect, because caused by the chorion mode of appearance and institutional framework of bat moth ovum and silkworm seed have differences.This method passes through dioxy
Embryo's integrality can be maintained by the chorion rapid-digestion of bat ovum, softening, decolouring by changing the reagent such as chlorine, ethanol, through place
Ovum after reason can carry out histopathologic slide.
Specifically, the invention provides the method that a kind of softening of host of Cordyceps sinensis bat moth chorion is decolourized, it is characterized in that
Bat moth ovum is immersed in the solution of 0.1-0.2% chlorine dioxide and handled 10 to 60 minutes.
In certain embodiments, the solution is the aqueous solution or ethanol water.
In certain embodiments, the ethanol water is 40%-70% ethanol waters.
In certain embodiments, the ethanol water is 50% ethanol water.
In certain embodiments, the solution is now with the current.
In certain embodiments, when the solution is aqueous solution of chlorine dioxide, processing time is 30-60 minutes.
In certain embodiments, when the solution is chlorine dioxide ethanol water, processing time is 15-30 minutes.
Present invention also offers a kind of host of Cordyceps sinensis bat moth chorion to soften decolorising agent, it is characterized in that 0.1-0.2%
The aqueous solution or ethanol water of chlorine dioxide.
In some embodiments of softening decolorising agent, the ethanol water is 40%-70% ethanol waters.
In other embodiments of softening decolorising agent, the ethanol water is 50% ethanol water.
The method that host of Cordyceps sinensis bat chorion softening provided by the invention is decolourized is simple to operate, controllable, and it softens
Decolorising agent composition is cheap, green, the existing technology for softening decolouring field for chorion is have updated completely, particularly to bat
The softening of moth chorion is decolourized, for bat moth ovum, further research provides possibility.
Brief description of the drawings
Fig. 1 is the chorion decolouring softening process schematic diagram of bat moth：A→B→C→D
Fig. 2 is early development bat ovum section schematic diagram
Fig. 3 is development mid-term bat ovum section schematic diagram
Embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified technological parameter realization.In particular,
All similar replacements and change are apparent to those skilled in the art, and they are considered as being included in the present invention
In.The present invention method be described by preferred embodiment, related personnel substantially can not depart from present invention,
Product as described herein and method are modified or suitably changed with combining in spirit and scope, to realize and using the present invention
Technology.
For a further understanding of the present invention, with reference to embodiment, the present invention is described in detail.
Embodiment 1 softens decolorization experiment
It is invested in 1.5-2 grams of chlorine dioxide powder (chlorine dioxide content 10%) in 100mL 50% ethanol water
(now with the current, absolute ethyl alcohol and water 1:1) bat moth ovum to be measured, is put into above-mentioned solution rapidly, ovum is gradually by black regression
Brown eventually become it is translucent faint yellow, after 15-30min, chorion softening decolourize complete.
The present invention is also handled bat moth chorion by using different softening agent and processing method, is found existing
Silkworm seed shell processing method is to bat moth ovum and does not apply to.
Table 1：Different softening agents handle bat moth ovum soft shell Contrast on effect：
Inventor does not obtain the soft of preferable bat moth ovum by being studied a variety of softening discoloration methods
Change discoloration method, after by the composition to bat moth chorion and the research of structure, test plurality of reagents, have finally chosen containing two
Softening decolorising agent of the solution of chlorine monoxid as bat moth ovum, have and preferably soften decolorizing effect；Different solvents are have studied again
The influence decolourized to softening, wherein the ClO 2 solution prepared with water and ethanol water can make chorion it is quick, effectively softening and
Fade, all without without vitellinae membrana breakoff phenomenon；Wherein with 50% ethanol water prepare chlorine dioxide decolorising agent can reach it is most fast most
Good softening decolorizing effect.
The histotomy of embodiment 2 is studied
1 gram of chlorine dioxide powder is taken to be dissolved in 60mL ethanol waters, ethanol:Water=2:1, take early development and mid-term
Bat moth ovum is put into above-mentioned solution, and ovum gradually changes colour, and ovum is changed into faint yellow after 30min, after the completion of softening of decolourizing, with 0.9% life
After managing saline rinse three times, histotomy is carried out.
Tissue section method：By ovum with Ka Nuoshi fixers (Carnoy`s Fluid) fix 24h, after routinely paraffin cut
The processing such as piece method is dehydrated, saturating wax and FFPE, it is tangentially 4.5 μM for rip cutting and slice thickness, (temperature is paster
42 DEG C) more than 1.5h (oven temperature is arranged to 55 DEG C), then the dyeing of row Hematoxylin-eosin counterstaining, neutral gum envelope are dried afterwards
Piece, section, which is placed in, to be come card DM2500 and is just putting micro- sem observation and taking pictures.
Conclusion：The bat moth ovum softened through this method can be smoothed out cutting into slices, and obtain good bat moth ovum embryo hair
Educate process slice map.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
1. a kind of method that host of Cordyceps sinensis bat moth chorion softening is decolourized, it is characterized in that bat moth ovum is immersed into 0.1-
Handled 10 to 60 minutes in the solution of 0.2% chlorine dioxide.
2. according to the method for claim 1, it is characterized in that the solution is the aqueous solution or ethanol water.
3. according to the method for claim 2, it is characterized in that the ethanol water is 40%-70% ethanol waters.
4. according to the method for claim 3, it is characterized in that the ethanol water is 50% ethanol water.
5. according to the method described in claim any one of 1-4, it is characterized in that the solution is now with the current.
6. according to the method described in claim any one of 1-2, it is characterized in that, when the solution is the aqueous solution, processing time
For 30-60 minutes.
7. according to the method described in claim any one of 1-4, it is characterized in that, when the solution is ethanol water, processing
Time is 15-30 minutes.
8. a kind of host of Cordyceps sinensis bat moth chorion softens decolorising agent, it is characterized in that the aqueous solution of 0.1-0.2% chlorine dioxide
Or ethanol water.
9. softening decolorising agent according to claim 8, the ethanol water solution is 40%-70% ethanol waters.
10. softening decolorising agent according to claim 8, the ethanol water is 50% ethanol water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710753975.5A CN107517931B (en) | 2017-08-29 | 2017-08-29 | A kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and softening decolorising agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710753975.5A CN107517931B (en) | 2017-08-29 | 2017-08-29 | A kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and softening decolorising agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107517931A true CN107517931A (en) | 2017-12-29 |
| CN107517931B CN107517931B (en) | 2019-11-12 |
Family
ID=60682622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710753975.5A Active CN107517931B (en) | 2017-08-29 | 2017-08-29 | A kind of host of Cordyceps sinensis bat moth chorion softening discoloration method and softening decolorising agent |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08289772A (en) * | 1995-04-24 | 1996-11-05 | Chisso Corp | Antimicrobial agent for food and sterilizing method |
| CN101676387A (en) * | 2008-09-19 | 2010-03-24 | 南阳师范学院 | Method for fixing a large number of silkworm embryos |
| CN101822237A (en) * | 2009-03-04 | 2010-09-08 | 北京七彩蝶创意文化有限公司 | Large-scale artificial breeding method of Cethosia biblis in north areas |
| CN106614387A (en) * | 2016-09-23 | 2017-05-10 | 王云 | Artificial cultivation method of cordyceps sinensis host insects |
-
2017
- 2017-08-29 CN CN201710753975.5A patent/CN107517931B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08289772A (en) * | 1995-04-24 | 1996-11-05 | Chisso Corp | Antimicrobial agent for food and sterilizing method |
| CN101676387A (en) * | 2008-09-19 | 2010-03-24 | 南阳师范学院 | Method for fixing a large number of silkworm embryos |
| CN101822237A (en) * | 2009-03-04 | 2010-09-08 | 北京七彩蝶创意文化有限公司 | Large-scale artificial breeding method of Cethosia biblis in north areas |
| CN106614387A (en) * | 2016-09-23 | 2017-05-10 | 王云 | Artificial cultivation method of cordyceps sinensis host insects |
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| Publication number | Publication date |
|---|---|
| CN107517931B (en) | 2019-11-12 |
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Effective date of registration: 20200526 Address after: Room 302, building 32, No. 368, Zhenan Middle Road, Chang'an Town, Dongguan City, Guangdong Province Co-patentee after: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Patentee after: Dongguan dongyangguang Cordyceps research and Development Co., Ltd Address before: 523808 Guangdong city of Dongguan province Hubei Songshan Industrial Park Industrial Road No. 1 Co-patentee before: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Patentee before: SUNSHINE LAKE PHARMA Co.,Ltd. |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to a softening and decolorizing method and a softening and decolorizing agent for the egg shell of Cordyceps sinensis host bat moth Effective date of registration: 20211119 Granted publication date: 20191112 Pledgee: Ronggui sub branch of Guangdong Shunde Rural Commercial Bank Co.,Ltd. Pledgor: Dongguan dongyangguang Cordyceps research and Development Co.,Ltd.|YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Registration number: Y2021980012842 |