CN107505427B - Thin-layer chromatography detection method for traditional Chinese medicine rheum palmatum - Google Patents
Thin-layer chromatography detection method for traditional Chinese medicine rheum palmatum Download PDFInfo
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- CN107505427B CN107505427B CN201710724752.6A CN201710724752A CN107505427B CN 107505427 B CN107505427 B CN 107505427B CN 201710724752 A CN201710724752 A CN 201710724752A CN 107505427 B CN107505427 B CN 107505427B
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- 238000004809 thin layer chromatography Methods 0.000 title claims abstract description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 34
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 33
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- 239000012488 sample solution Substances 0.000 claims abstract description 14
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- 238000000605 extraction Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 229910002027 silica gel Inorganic materials 0.000 claims description 10
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- 239000000523 sample Substances 0.000 claims description 9
- 238000011161 development Methods 0.000 claims description 8
- 229910021529 ammonia Inorganic materials 0.000 claims description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 4
- 238000002481 ethanol extraction Methods 0.000 claims description 4
- PQPKPCPUSAPERC-UHFFFAOYSA-N ethyl acetate;formic acid;hexane Chemical compound OC=O.CCCCCC.CCOC(C)=O PQPKPCPUSAPERC-UHFFFAOYSA-N 0.000 claims description 4
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 3
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
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- 206010010774 Constipation Diseases 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
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- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
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- AVKAJIUWUFOCCN-UHFFFAOYSA-N dichloromethane;ethanol;hexane Chemical compound CCO.ClCCl.CCCCCC AVKAJIUWUFOCCN-UHFFFAOYSA-N 0.000 description 1
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- WBJINCZRORDGAQ-UHFFFAOYSA-N formic acid ethyl ester Natural products CCOC=O WBJINCZRORDGAQ-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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CN112684096B (en) * | 2021-01-29 | 2023-04-28 | 浙江省立同德医院 | Method for identifying radix tetrastigme medicinal material based on polyamide thin-layer chromatography |
CN113552275B (en) * | 2021-06-24 | 2022-07-05 | 海南医学院 | Identification method of bat grass |
CN113295817A (en) * | 2021-06-30 | 2021-08-24 | 江西农业大学 | Preparation, separation and identification method of phoenix-tail fern bacteriostatic component |
CN114924022B (en) * | 2022-05-13 | 2023-12-19 | 华润三九医药股份有限公司 | Thin-layer identification method of rheum officinale product and application thereof |
CN115586290A (en) * | 2022-08-31 | 2023-01-10 | 陕西嘉禾生物科技股份有限公司 | Method for rapidly identifying dioscorea zingiberensis diosgenin and dioscorea mexicana saponin |
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CN101002905A (en) * | 2007-01-12 | 2007-07-25 | 广东众生药业股份有限公司 | Medicine for treating infantile anorexia, its preparing method and quality control method |
CN101732607B (en) * | 2010-01-05 | 2014-04-02 | 贵州信邦制药股份有限公司 | Method for detecting quality of huaqi Chinese medicinal preparation |
CN102692466A (en) * | 2012-03-29 | 2012-09-26 | 广州星群(药业)股份有限公司 | Detection method of Jizhong Tincture |
CN103408613B (en) * | 2013-07-22 | 2016-01-20 | 中国科学院西北高原生物研究所 | The preparation method of anthraquinone glycoside and stilbene glucoside chemical reference substance in rhubarb medicinal material |
CN103690791B (en) * | 2013-12-25 | 2016-04-20 | 咸阳长涛电子科技有限公司 | A kind of Chinese medicine composition and preparation and determination methods method thereof being used for the treatment of chronic gastritis |
CN104740312B (en) * | 2013-12-25 | 2018-04-10 | 咸阳长涛电子科技有限公司 | A kind of Chinese medicine composition and its preparation and detection method for being used to treat chronic gastritis |
CN105138861B (en) * | 2015-05-31 | 2018-10-12 | 青岛市食品药品检验研究院 | A kind of construction method of the rhubarb medicinal material true and false and base source kind class prediction model |
CN106198840A (en) * | 2016-08-12 | 2016-12-07 | 成都维恒医药科技有限公司 | Developing solvent and thin-layer identification method for Chinese crude drug Radix Et Rhizoma Rhei thin-layer identification method |
CN106511512A (en) * | 2016-11-23 | 2017-03-22 | 北京市中医研究所 | Traditional Chinese medicine external-use gel and preparation method and application thereof |
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Effective date of registration: 20201021 Address after: 322000 1st floor, No.3, building 20, Qiyi village, Chengxi street, Yiwu City, Jinhua City, Zhejiang Province Applicant after: Yiwu Lansi sporting goods Co.,Ltd. Address before: Room 501, No. 85, Xiaguang Dongli, Haicang District, Xiamen City, Fujian Province Applicant before: Xiamen reliable intellectual property service Co.,Ltd. |
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Effective date of registration: 20240425 Address after: Room 2A, Science and Technology Building, No. 968 Xuefeng West Road, Beiyuan Street, Yiwu City, Jinhua City, Zhejiang Province, 322000 Patentee after: Zhejiang Jinguo Intellectual Property Co.,Ltd. Country or region after: China Address before: 322000 1st floor, No.3, building 20, Qiyi village, Chengxi street, Yiwu City, Jinhua City, Zhejiang Province Patentee before: Yiwu Lansi sporting goods Co.,Ltd. Country or region before: China |
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