CN107502561A - Coronoid process dissipate capsule bacterium and its application, black tea and its processing method - Google Patents
Coronoid process dissipate capsule bacterium and its application, black tea and its processing method Download PDFInfo
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Abstract
The present invention relates to a kind of coronoid process dissipate capsule bacterium and its application, black tea and its processing method, belong to microorganism and technical field of food biotechnology.The biological deposits of the coronoid process dissipate capsule bacterium are numbered：CCTCC NO：M 2017355.Above-mentioned coronoid process dissipate capsule bacterium is processed applied to black tea, the rate of growing dim of black tea can be improved, promote the conversion of polyphenol substance.The processing method of black tea comprises the following steps：By tea raw material of the bacterial suspension inoculation of the spore containing above-mentioned coronoid process dissipate capsule bacterium after pile fermentation, fermentation.The problem of processing method is simple to operation, and cost is low, quality of finished product good, can solve the problem that existing black tea, and especially Fu-brick tea drinks inconvenience.Thus black tea obtained by processing is grown dim stably, and polyphenol substance conversion ratio is higher, and millet paste color and luster is preferable.
Description
Technical field
The present invention relates to microorganism and technical field of food biotechnology, and more particularly to a kind of coronoid process dissipate capsule bacterium and its application,
Black tea and its processing method.
Background technology
Fu-brick tea is the black tea product that one of black tea has much characteristic, is the reprocessing tea that black tea is formed after overcompaction
Class, belong to post-fermented tea, there are the various health care functions such as lowering blood-fat and reducing weight, anti-oxidant, antibacterial, antitumor and regulation immunologic function,
Had deep love for extensively by consumer.
Dominant strain " coronoid process dissipate capsule bacterium " largely produces during growing dim in Fu-brick tea, to Fu-brick tea qualitative effects very
Greatly, its content is to evaluate the important indicator of Fu-brick tea quality.The production of Fu-brick tea at present is sent out naturally by completion of growing dim naturally
The cycle is spent to grow, and it is uneven easily to grow dim, it is very big to Fu-brick tea qualitative effects.Commercially available Fu-brick tea is pressed into brick, and weight is larger,
The problem of with inconvenience is drunk.
The content of the invention
The first object of the present invention is to provide a kind of coronoid process dissipate capsule bacterium, and the coronoid process dissipate capsule bacterium activity is higher, can be used in
Inoculation, which is grown dim, produces black tea.
The second object of the present invention can improve black in providing a kind of application of above-mentioned coronoid process dissipate capsule bacterium in black tea processing
The rate of growing dim of tea, promotes the conversion of polyphenol substance.
The third object of the present invention is to provide a kind of processing method of black tea, and the processing method is simple to operation, cost
It is low, quality of finished product good, it can solve the problem that the problem of Fu-brick tea drinks inconvenience in existing black tea.
The fourth object of the present invention is that providing one kind black tea, the black tea as obtained by the processing of above-mentioned processing method grows dim surely
Fixed, polyphenol substance conversion ratio is higher, and millet paste color and luster is preferable.
The present invention is solved its technical problem and realized using following technical scheme：
The present invention proposes a kind of coronoid process dissipate capsule bacterium, and its biological deposits numbering is：CCTCC NO：M 2017355, the coronoid process dissipate
Capsule bacterium is stored in China typical culture collection center (CCTCC) on June 21st, 2017, and address is big for the Wuhan of Wuhan, China
Learn.
The present invention also proposes a kind of application of above-mentioned coronoid process dissipate capsule bacterium in black tea processing, it is preferable that above-mentioned black tea is Fu
Brick tea.
The present invention also proposes a kind of processing method of black tea, comprises the following steps：By the spore containing above-mentioned coronoid process dissipate capsule bacterium
Tea raw material of the bacterial suspension inoculation of son after pile fermentation, fermentation.
Preferably, above-mentioned black tea is Fu-brick tea.
The present invention also proposes a kind of black tea, is processed by the processing method of above-mentioned black tea and is obtained.
Coronoid process dissipate capsule bacterium and its application, the beneficial effect of black tea and its processing method are in present pre-ferred embodiments：
Coronoid process dissipate capsule bacterium activity provided in an embodiment of the present invention is higher, can be used in inoculation grow dim production black tea.Will be above-mentioned
Coronoid process dissipate capsule bacterium is applied in black tea processing, can improve the rate of growing dim of black tea, promote the conversion of polyphenol substance.
In addition, the processing method of black tea provided in an embodiment of the present invention is simple to operation, cost is low, contains hat by inoculation
Prominent bulk bacteria PW-1 bacteria suspension, the rate of growing dim of black tea on the one hand can be improved, black tea is grown dim stably, on the other hand, can
Promote polyphenol substance in black tea to convert, improve millet paste color and luster, in addition, above-mentioned processing method can also solve existing black tea, especially
It is the problem of Fu-brick tea drinks inconvenience.Through black tea obtained by the processing of above-mentioned processing method, stabilization of growing dim, polyphenol substance conversion ratio
Higher, millet paste color and luster is preferable, quality of finished product good.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below by embodiment it is required use it is attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is that the biological deposits information of coronoid process dissipate capsule bacterium provided in an embodiment of the present invention and survival prove；
Fig. 2 is the morphological feature figure of coronoid process dissipate capsule bacterium provided in an embodiment of the present invention；
Fig. 3 is the 18S rDNA phylogenetic trees structure figure of coronoid process dissipate capsule bacterium PW-1 bacterial strains provided in an embodiment of the present invention；
Fig. 4 is total phenol content of the Fu-brick tea of test group and control group under the different fermentations time in test example 1 of the present invention
Figure；
Fig. 5 is the mass percent of catechin in sample 1-3 and sample 4-7 Fu-brick tea in test example 2 of the present invention；
Fig. 6 is the mass percent of catechin in sample 1-3 and sample 8-11 Fu-brick tea in test example 2 of the present invention.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
Coronoid process dissipate capsule bacterium and its application, the black tea and its processing method of the embodiment of the present invention are specifically described below.
Coronoid process dissipate capsule bacterium provided in an embodiment of the present invention is isolated and purified and obtained in the following manner：Military Fu brick teas of making even carry
The tealeaves or tea stalk of " golden flower " (coronoid process dissipate capsule bacterium) are inoculated in PDA culture medium, and above-mentioned " golden flower " is derived from the different parts of Fu brick teas.
It is incubated under conditions of 28 DEG C after inoculation, treat that bacterium colony is grown, choosing colony is rule in new PDA culture medium and purified, until
Single bacterium colony is obtained, preserves the strain.
What deserves to be explained is above-mentioned " golden flower " is coronoid process dissipate capsule bacterium, it can secrete a variety of ectoenzymes, can promote egg in tealeaves
The degraded of white matter, and polyphenol compound oxidation can be catalyzed so as to be converted into the material beneficial to human body, improve the mouthfeel of millet paste
And effect.
Identified, the strain in the embodiment of the present invention belongs to coronoid process dissipate capsule bacterium, and specific name is coronoid process dissipate capsule bacterium PW-1
(Eurothum cristatum PW-1), and on June 21st, 2017 in the Chinese Typical Representative culture positioned at Wuhan, China university
Collection preservation, deposit number are CCTCC NO：M 2017355.The biological deposits of coronoid process dissipate capsule bacterium used in the present embodiment
Information and survival proof refer to Fig. 1.
Morphological observation is carried out to the bacterium, specifically, using 3 inocalation methods, by isolated coronoid process dissipate capsule bacterium PW-1
Respectively at cultivating 21d (under conditions of 28 DEG C incubated) in PDA culture medium and CA culture mediums and observe growing state.Its
In, the compound method of PDA culture medium can be as follows：Take 5g potato leaching powders, 20g glucose, 20g agar and 0.1g chloramphenicol with
1L water is obtained by mixing.The compound method of CA culture mediums can be as follows：Take 3g natrium nitrosums, 1g dipotassium hydrogen phosphates, 0.5g magnesium sulfate,
0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose and 20g agar mix with 1L water, adjust culture medium system pH to
5.0-5.5。
Fig. 2A and Fig. 2 B are refer to, wherein, Fig. 2A is bacterium colony when coronoid process dissipate capsule bacterium PW-1 cultivates 7d in PDA culture medium
Front aspect graph, Fig. 2 B are the reverse side aspect graph of bacterium colony when coronoid process dissipate capsule bacterium PW-1 cultivates 7d in PDA culture medium.Through cultivating,
Growing states of the coronoid process dissipate capsule bacterium PW-1 in PDA culture medium is as described below：When cultivating 2d bacterium colony is grown in culture medium.Bacterium during 7d
Fall a diameter of 15-16mm.Colony diameter is 26-29mm during 14d, and shape subcircular, central protuberance, bacterium colony periphery has a circle white
To light yellow velvet shape mycelium, core color is deeper, nearly olive-drab；Periphery of bacterial colonies has obvious golden yellow pigment to expand
Dissipate in matrix, a large amount of yellow tool decorations mycelia are presented, become brown after old.Colony diameter is 38-41mm during 21d, colony colour
Deepen, periphery has no conidium almost without mycelia；Bacterium colony rough surface is fine and close, is with a small number of golden yellow spots, the bacterium colony back of the body outside
There is an indent gully in face, and pigment is by whole culture medium stained yellow.
Fig. 2 C and Fig. 2 D are refer to, wherein, Fig. 2 C are bacterium colony when coronoid process dissipate capsule bacterium PW-1 cultivates 7d in CA culture mediums
Front aspect graph, Fig. 2 D are the reverse side aspect graph of bacterium colony when coronoid process dissipate capsule bacterium PW-1 cultivates 7d in CA culture mediums.Coronoid process dissipates capsule
Growing states of the bacterium PW-1 in CA culture mediums is as described below：Start slow-growing, colony diameter is 17-21mm during 7d, during 14d
Colony diameter is 27-29mm；Bacterium colony subcircular, flat finer and close, peripheral velvet shape mycelia is light yellow in olive, face among bacterium colony
The more peripheral depth of color, old rear bacterium colony are gradually changed into brown from yellow.Increase with incubation time, bacterium colony surface produces conidium knot
Structure, brown, bacterial strain produces xanthein and is spread in matrix, bacterium colony reverse side yellowish-brown.
Further, in order to effectively be observed coronoid process dissipate capsule bacterium PW-1 spore, will also divide in the embodiment of the present invention
It is (under conditions of 28 DEG C permanent respectively at cultivating 21d in CZ20 culture mediums and CZ40 culture mediums from obtained coronoid process dissipate capsule bacterium PW-1
Temperature culture) and observe growing state.It is preferred that take on tri- kinds of culture mediums of CA, CZ20 and CZ40 that incubation time is 4d to 7d
Bacterial strain micro- sem observation is carried out under light microscope.
Concrete operations are as follows：In in clean glass slide, 1-2 drop lactophenol cotton blue dyeing liquors are dripped, with oese from bacterium colony side
Take a small amount of mycelia with spore to be placed in dyeing liquor outside edge, then mycelia is chosen and scattered, covered is under microscope from low
Times mirror is observed to high power lens, and is photographed to record.As shown in Fig. 2 E- Fig. 2 H, Fig. 2 E- Fig. 2 H represent optics and shown its result respectively
Coronoid process dissipate capsule bacterium PW-1 mycelia, conidiophore, ascocarp and ascospore aspect graph (× 40) under micro mirror.The following institute of form
State：Coronoid process dissipate capsule bacterium PW-1 mitogenetic falx stem is longer, and wall is smooth；Mycelia has every in asymmetric branch；Top capsule is in flask shape,
Bottle stalk is born in the top capsule first half；Conidium ellipse, it is a small number of subsphaeroidal；Ascospore ellipse is in yellow, and mitogenetic spore
Subheader largely mixes, and how spherical in shape ascocarp is.
Further, the thalline on tri- kinds of culture mediums of CA, CZ20 and CZ40 that incubation time is 7d is collected in scanning electricity
Microscopic observation.Wherein, unique difference of CZ20 culture mediums and CA culture mediums in the former culture medium cane sugar content be 200g,
Unique difference of CZ40 culture mediums and CA culture mediums in the former culture medium cane sugar content be 400g.The concrete operations of microscopy are such as
Under：With 0.1mol/L phosphate buffer (Na+) washing thalline, 4wt% glutaraldehyde solutions are added after centrifugation, in 4 DEG C of condition
Lower fixed 8-20h, is centrifuged again, and adds 2wt% glutaraldehyde solutions, fixes 1-2h, Ran Houyong again under conditions of 4 DEG C
0.1mol/L phosphate buffer washing, centrifugation, collects thalline.Successively with 30wt%, 50wt%, 70wt%, 85wt%,
95wt% and 100wt% ethanol solution carries out serial dehydration to thalline obtained by collection, 10-15min is eluted every time, in CO2
Microscopy after critical drying.
Its result as shown in Fig. 2 I- Fig. 2 L, wherein Fig. 2 I- Fig. 2 K represent respectively ESEM multiple be respectively 3000,
3000 and the aspect graph of 2000 times coronoid process dissipate capsule bacterium PW-1 conidiophores, Fig. 2 L be that ESEM multiple is that 7000 times coronoid process dissipate
The conidial aspect graphs of capsule bacterium PW-1.Form is as described below：In the vigorous aerial hyphae of coronoid process dissipate capsule bacterium PW-1 strain growths
There is substantial amounts of mitogenetic falx stem structure, wall is smooth, and it is 58 μm of 64-140 μ ms to measure size；Sporophore end top capsule is in flask shape
Or subsphaeroidal, 10-21 μm of diameter, big portion surface are fertile；Upright connect of bottle produces from top capsule, upper half of the dense distribution in top capsule
Portion, 1.8-3 μm of size 3-8.5 μ ms, conidial fructification individual layer；After bottle stalk is ripe, conidium directly obstructs top to sprout from bottle
Mode is developed, and with conidial maturation, the conidium of lower end germinates, and has spore form appearance of concatenating, production spore position
Put constant, come off after ripe, have hole at bottle stalk；Conidium is oval, and both ends are truncate, rough surface, has spinule, and size is
4-5.5μm×3.2-4.3μm；It is in subsphaeroidal during conidial head children, is in loose radiation after old, size is 25-60 μm, Electronic Speculum
Under do not observe zoogamy structure.
Further, molecular biology identification is carried out to the 18S rDNA of coronoid process dissipate capsule bacterium PW-1 bacterial strains, specifically include as
Lower step：Expanded from primer NS1 and NS8, row agarose gel electrophoresis detection is entered to product, ultraviolet imagery system is clapped
According to；PCR primer after purification is subjected to sequencing, is as a result committed in ncbi database and carries out homologous comparison, is chosen homologous
The higher sequence of property, is analyzed sequence using MEGA analysis softwares, and carries out phylogenetic tree structure, its result such as Fig. 3
It is shown.Wherein, primer NS1 and primer NS8 base sequence are respectively：5 '-GTAGTCATATGCTTGTCTC-3 ' and 5 '-
TCCGCAGGTTCACCTACGGA-3’.As seen from Figure 3, coronoid process dissipate capsule bacterium PW-1 bacterial strains and coronoid process dissipate capsule bacterium homology be most
Height, similitude 99%.Bacterial strain PW-1 morphological feature and molecular biology identification result in the comprehensive embodiment of the present invention, really
Fixed bacterial strain of the present invention is coronoid process dissipate capsule bacterium, and is named as above-mentioned coronoid process dissipate capsule bacterium PW-1.
The above-mentioned coronoid process dissipate capsule bacterium PW-1 being isolated can be applied in black tea processing, to improve the rate of growing dim of black tea, promote
Enter the conversion of polyphenol substance.Optionally, the tea raw material in the embodiment of the present invention can for example include Fu-brick tea tealeaves, health
Brick tea tealeaves, the sharp tea tealeaves of gold and six fort tea tealeaves etc..Preferably, coronoid process dissipate capsule bacterium is applied to the Fu-brick tea in black tea, can be bright
The aobvious rate of growing dim for improving Fu-brick tea, makes Fu-brick tea stably grow dim.
A kind of processing method of black tea is additionally provided in the embodiment of the present invention, such as may comprise steps of：It will contain
Tea raw material of the bacterial suspension inoculation of above-mentioned coronoid process dissipate capsule bacterium PW-1 spore after pile fermentation, fermentation.Preferably, above-mentioned black tea is
Fu-brick tea.
Wherein, bacteria suspension is obtained by mixing by the spore of sterilized water and coronoid process dissipate capsule bacterium PW-1.Preferably, every milliliter of bacteria suspension
In contain 1 × 106-2×106Individual coronoid process dissipate capsule bacterium PW-1 spore；More preferably, 1.5 × 10 are contained in every milliliter of bacteria suspension6It is individual
Coronoid process dissipate capsule bacterium PW-1 spore.It is preferred that every kilogram of tea raw material inoculation above-mentioned bacteria suspension of 40-80mL, more preferably, every kilogram
Tea raw material is inoculated with the above-mentioned bacteria suspensions of 60mL.
Pile fermentation can for example stack 16-20h under conditions of 45-55 DEG C, stack 18h under conditions of being preferable over 50 DEG C.This hair
In bright embodiment, before pile fermentation, in addition to by tea raw material under conditions of 95-100 DEG C decatize 20-30min, be preferable over 100 DEG C
Under conditions of decatize 25min, the soft stingless feel of the tealeaves food value of leaf after this preferred Steaming conditions decatize.
After being inoculated with bacteria suspension, fermentation can be carried out 20-22 days under conditions of 26-30 DEG C, permanent under conditions of being preferable over 28 DEG C
Temperature fermentation 21d.
With fermentation condition the degree of growing dim of black tea, especially Fu-brick tea can be made to reach optimal by above-mentioned preferable inoculum concentration,
The conversion ratio of polyphenol substance can also be improved simultaneously.Also, by above-mentioned processing method, the black tea weight of gained can be avoided larger,
Unfavorable the problem of brewing and drinking.What deserves to be explained is according to different black tea kinds and concrete condition, it can also pass through regulation
Inoculum concentration and fermentation time in process control the degree of growing dim of black tea.
The embodiment of the present invention additionally provides the black tea as obtained by the processing of above-mentioned processing method, and the black tea is grown dim stably, polyphenol
Material conversion ratio is higher, and millet paste color and luster is orange bright, quality better.In addition, the relatively common common black tea of the black tea of gained is smaller
Type, namely weight are lighter, beneficial to brewing and drink.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Sterilized water is mixed with coronoid process dissipate capsule bacterium PW-1 spore, obtains bacteria suspension.Contain 1 in every milliliter of above-mentioned bacteria suspension
×106Individual coronoid process dissipate capsule bacterium PW-1 spore.
Health brick tea tealeaves decatize 30min under conditions of 95 DEG C is taken, then the pile fermentation 20h under conditions of 45 DEG C.
It is inoculated with the surface of the health brick tea tealeaves of pile fermentation in the ratio of every kilogram of tealeaves inoculation above-mentioned bacteria suspension of 40mL.
Then fermented 22 days under conditions of 26 DEG C, obtain finished product health brick tea.
Embodiment 2
Sterilized water is mixed with coronoid process dissipate capsule bacterium PW-1 spore, obtains bacteria suspension.Contain 2 in every milliliter of above-mentioned bacteria suspension
×106Individual coronoid process dissipate capsule bacterium PW-1 spore.
The sharp tea tealeaves decatize 20min under conditions of 100 DEG C of gold is taken, then the pile fermentation 16h under conditions of 55 DEG C.
It is inoculated with the surface of the golden sharp tea tealeaves of pile fermentation in the ratio of every kilogram of tealeaves inoculation above-mentioned bacteria suspension of 80mL.
Then fermented 20 days under conditions of 30 DEG C, obtain the sharp tea of finished product gold.
Embodiment 3
Sterilized water is mixed with coronoid process dissipate capsule bacterium PW-1 spore, obtains bacteria suspension.Contain in every milliliter of above-mentioned bacteria suspension
1.5×106Individual coronoid process dissipate capsule bacterium PW-1 spore.
Six fort tea tealeaves decatize 25min under conditions of 98 DEG C are taken, then the pile fermentation 18h under conditions of 50 DEG C.
It is inoculated with the surface of six fort tea tealeaves of pile fermentation in the ratio of every kilogram of tealeaves inoculation above-mentioned bacteria suspension of 60mL.
Then fermented 21 days under conditions of 28 DEG C, obtain the fort tea of finished product six.
Embodiment 4
Sterilized water is mixed with coronoid process dissipate capsule bacterium PW-1 spore, obtains bacteria suspension.Contain in every milliliter of above-mentioned bacteria suspension
1.5×106Individual coronoid process dissipate capsule bacterium PW-1 spore.
Fu-brick tea tealeaves decatize 25min under conditions of 100 DEG C is taken, then the pile fermentation 18h under conditions of 50 DEG C.
It is inoculated with the surface of the Fu-brick tea tealeaves of pile fermentation in the ratio of every kilogram of tealeaves inoculation above-mentioned bacteria suspension of 60mL.
Then fermented 21 days under conditions of 28 DEG C, obtain finished product Fu-brick tea.
Test example 1
Repeat to implement above-described embodiment 1-4, obtain enough finished product health brick teas, the sharp tea of finished product gold, the fort tea of finished product six and
Finished product Fu-brick tea.Using embodiment 4 as test group, control group is set, the difference of control group and test group be control group with etc.
Measuring sterilized water replaces the bacteria suspension in test group to carry out spontaneous fermentation.The Fu-brick tea of contrast test group and control group is in different fermentations
Total phenol content (mg/g) under time, total phenol content measure are pressed《GB/T 8313-2008 Tea Polyphenols in Tea and catechin contain
The detection of amount》Method is measured, and its result is as shown in Figure 4." * * " represent test group and control group under the fermentation time in Fig. 4
Sample between difference there is pole significant difference.
As seen from Figure 4, increase with fermentation time, the total phenol content of control group and test group is presented on the whole to be declined
Trend, and under same fermentation time, the total phenol content of the total phenol content of test group Fu-brick tea compared with control group Fu-brick tea is lower, says
Bright test group is higher compared with the polyphenol substance conversion ratio of the Fu-brick tea of control group.
Test example 2
By taking embodiment 4 as an example, set 1-11 sample sets, wherein sample 1 be embodiment 4 Fu-brick tea tea raw material, sample 2
For the tea raw material after decatize 25min in embodiment 4, sample 3 is the tea raw material after pile fermentation 18h in embodiment 4, sample 4 to
Sample 7 is respectively Fu-brick tea of the embodiment 4 after spontaneous fermentation 7d, 10d, 15d and 21d, and sample 8 to sample 11 is respectively to implement
Fu-brick tea of the example 4 after inoculation fermentation (bacteria suspension for being inoculated with the spore containing coronoid process dissipate capsule bacterium PW-1) 7d, 10d, 15d and 21d.
The mass percent (%) of catechin in the Fu-brick tea of comparative sample 1 to 11, catechin content measure are surveyed by the following method
It is fixed.Specifically, sample pretreatment and efficient liquid phase chromatographic analysis condition are as follows in assay method：
(1) sample pretreatment
Sample, solid-liquid ratio 1 are extracted with 70wt% methanol aqueous solution：20, extraction time 12h, surpass in extraction process
Sonication 3 times, each 20min, constant volume after decompression filters, it is standby to cross 0.22 μm of filter membrane.
(2) efficient liquid phase chromatographic analysis condition
ODS-3 chromatographic columns (4.6 × 250mm, 5 μm)；Mobile phase A：Water (0.1wt% formic acid)；Mobile phase B：Methanol；Stream
Speed：1mL/min；Column temperature：30℃；Sample size：20μL；Ultraviolet detection wavelength：280nm.
Time elution program is as shown in table 1.
Table 1HPLC elution programs
Measurement result is as shown in Figures 5 and 6.Wherein, GA represents gallic acid, and EGC represents epigallocatechin, C generations
Epicatechin, EGCG represent Epigallo-catechin gallate (EGCG), and EC represents epicatechin, and ECG represents epicatechin and do not eaten
Sub- acid esters.
From Fig. 5 and Fig. 6, under spontaneous fermentation and inoculation fermentation two ways, the catechin total amount contained by Fu-brick tea is equal
It is on a declining curve, and the catechin total amount decline degree of inoculation fermentation sample is bigger.GA, EGC, C, EGCG, EC, ECG content
Downward trend is integrally presented in whole fermentation process, and above-mentioned catechins decline degree more in inoculation fermentation sample
Greatly.EGC content is higher compared with other several content of material, and the variation tendency in spontaneous fermentation sample and inoculation fermentation sample
It is identical.C is relatively low in spontaneous fermentation sample and content in inoculation fermentation sample.Contrast spontaneous fermentation sample and inoculation fermentation sample
Result understand that with the increase of attenuation degree, catechins sustaining degradation, content declines, and coronoid process dissipate capsule bacterium PW-1
The degraded of catechins can be promoted.This result may largely secrete ectoenzyme during the fermentation with microorganism, promote
Enzymatic oxidation occurs for catechins, declines its content relevant.
Test example 3
The sample 1 to 11 in test example 2 is taken, it is brewed under the same terms.Above-mentioned brewing conditions are as follows：It is 1 by solid-liquid ratio：
50 add boiling water in sample brews 10min.Constant volume is standby after millet paste decompression is filtered, and preheats color difference meter and prepares blank sample
Product, take the millet paste of 2/3 cuvette volume to carry out value of chromatism measure after correction, united using Hunter Lab colour systems, each sample weight
It is multiple to average three times, L*, a*, b* value of the millet paste obtained by different samples are measured, wherein, L* is brightness value, and a* is
Red scale value, b* are yellow value degree, and measurement result is as shown in table 2.
The millet paste color and luster of table 2
Wherein, △ E are total color difference value.As can be seen from Table 2, when the reddish yellow degree of the Fu-brick tea millet paste after brewed is with fermentation
Between increase and increase, and inoculation fermentation group (sample 8-11) is bigger compared with spontaneous fermentation group (sample 4-7) increasing degree.
From the above, the processing method of black tea provided in an embodiment of the present invention, coronoid process dissipate capsule bacterium PW-1 is contained by inoculation
Bacteria suspension, on the one hand can improve the rate of growing dim of Fu-brick tea, and the stabilization that can make to grow dim.Through growing dim obtained by above-mentioned processing method
Golden flower quantity in the fermentation Fu-brick tea of the 7th day is much larger than《GB/T 9833.3-2013 compressed tea third portion Fu-brick teas》Middle hat
Prominent bulk bacteria >=20 × 104CFU/g requirement.On the other hand, by being inoculated with the bacteria suspension containing coronoid process dissipate capsule bacterium PW-1, moreover it is possible to
Promote polyphenol substance in Fu-brick tea to convert, improve millet paste color and luster.
In addition, being tested respectively embodiment 1-3 according to test example 1-3 method, its result shows inoculation fermentation group
Compared with spontaneous fermentation group tea quality more preferably.Therefore, black tea processing method provided in an embodiment of the present invention is compared with common process side
Black tea obtained by method (spontaneous fermentation) has higher quality and advantage.
In summary, the coronoid process dissipate capsule bacterium activity of the embodiment of the present invention is higher, can be used in inoculation grow dim production black tea.Will
Above-mentioned coronoid process dissipate capsule bacterium is applied in black tea processing, can improve the rate of growing dim of black tea, promote the conversion of polyphenol substance.In addition, this
The processing method for the black tea that inventive embodiments provide is simple to operation, and cost is low, quality of finished product good, can solve the problem that existing Fu bricks
Tea-drinking with it is inconvenient the problem of.Thus black tea obtained by processing, stabilization of growing dim, polyphenol substance conversion ratio is higher, and millet paste color and luster is preferable.
Embodiments described above is part of the embodiment of the present invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
SEQUENCE LISTING
<110>Sichuan University
<120>Coronoid process dissipate capsule bacterium and its application, black tea and its processing method
<130> PA17042104SC
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1555
<212> DNA
<213>Coronoid process dissipate capsule bacterium PW-1
<400> 1
taccttacta catggatacc tgtggtaatt ctagagctaa tacatgctaa aaacctcgac 60
ttcggaaggg gtgtatttat tagataaaaa accaacgccc ttcggggctc cttggtgaat 120
cataataact aagcgaatcg catggccttg cgccggcgat ggttcattca aatttctgcc 180
ctatcaactt tcgatggtag gatagtggcc taccatggtg gcaacgggta acggggaatt 240
agggttcgat tccggagagg gagcctgaga aacggctacc acatccaagg aaggcagcag 300
gcgcgcaaat tacccaatcc cgacacgggg aggtagtgac aataaatact gatacggggc 360
tcttttgggt ctcgtaattg gaatgagtac aatctaaatc ccttaacgag gaacaattgg 420
agggcaagtc tggtgccagc agccgcggta attccagctc caatagcgta tattaaagtt 480
gttgcagtta aaaagctcgt agttgaacct tgggtctggc tggccggtcc gcctcaccgc 540
gagtactggt ccggctggac ctttccttct ggggaacctc atggccttca ctggctgtgg 600
ggggaaccag gacttttact gtgaaaaaat tagagtgttc aaagcaggcc tttgctcgaa 660
tacattagca tggaataata gaataggacg tgcggttcta ttttgttggt ttctaggacc 720
gccgtaatga ttaataggga tagtcggggg cgtcagtatt cagctgtcag aggtgaaatt 780
cttggatttg ctgaagacta actactgcga aagcattcgc caaggatgtt ttcattaatc 840
agggaacgaa agttagggga tcgaagacga tcagataccg tcgtagtctt aaccataaac 900
tatgccgact agggatcggg cggtgtttct ataatgaccc gctcggcacc ttacgagaaa 960
tcaaagtttt tgggttctgg ggggagtatg gtcgcaaggc tgaaacttaa agaaattgac 1020
ggaagggcac cacaaggcgt ggagcctgcg gcttaatttg actcaacacg gggaaactca 1080
ccaggtccag acaaaataag gattgacaga ttgagagctc tttcttgatc ttttggatgg 1140
tggtgcatgg ccgttcttag ttggtggagt gatttgtctg cttaattgcg ataacgaacg 1200
agacctcggc ccttaaatag cccggtccgc atttgcgggc cgctggcttc ttagggggac 1260
tatcggctca agccgatgga agtgcgcggc aataacaggt ctgtgatgcc cttagatgtt 1320
ctgggccgca cgcgcgctac actgacaggg ccagcgagta catcaccttg gccgagaggt 1380
ccgggtaatc ttgttaaacc ctgtcgtgct ggggatagag cattgcaatt attgctcttc 1440
aacgaggaat gcctagtagg cacgagtcat cagctcgtgc cgattacgtc cctgcccttt 1500
gtacacaccg cccgtcgcta ctaccgattg aatggctcgg tgaggccttc ggact 1555
Claims (10)
1. a kind of coronoid process dissipate capsule bacterium, it is characterised in that its biological deposits, which is numbered, is：CCTCC NO：M 2017355.
2. application of the coronoid process dissipate capsule bacterium as claimed in claim 1 in black tea processing.
3. application of the coronoid process dissipate capsule bacterium in black tea processing according to claim 2, it is characterised in that the black tea is Fu bricks
Tea.
4. a kind of processing method of black tea, it is characterised in that comprise the following steps：Coronoid process as claimed in claim 1 will be contained
Tea raw material of the bacterial suspension inoculation of the spore of bulk bacteria after pile fermentation, fermentation；
Preferably, the black tea is Fu-brick tea.
5. processing method according to claim 4, it is characterised in that the bacteria suspension dissipates capsule by sterilized water and the coronoid process
The spore of bacterium is obtained by mixing；
Preferably, 1 × 10 is contained in every milliliter of bacteria suspension6-2×106The spore of the individual coronoid process dissipate capsule bacterium.
6. processing method according to claim 4, it is characterised in that every kilogram of tea raw material inoculation 40-80mL institute
State bacteria suspension.
7. processing method according to claim 4, it is characterised in that pile fermentation is that 16- is stacked under conditions of 45-55 DEG C
20h。
8. processing method according to claim 4, it is characterised in that before pile fermentation, by the tea raw material in 95-100 DEG C
Under conditions of decatize 20-30min.
9. processing method according to claim 4, it is characterised in that fermentation is that 20-22 is carried out under conditions of 26-30 DEG C
My god.
A kind of 10. black tea, it is characterised in that the black tea as described in claim any one of 4-9 processing method processing and
.
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| CN113115893A (en) * | 2019-12-31 | 2021-07-16 | 湖南湘源美东医药科技有限公司 | Food antibacterial additive and application thereof |
| CN113249233A (en) * | 2021-06-29 | 2021-08-13 | 北京百奥茵诺生物科技有限公司 | Eurotium cristatum and microbial inoculum and application thereof |
| CN114431312A (en) * | 2022-01-24 | 2022-05-06 | 浙江欣诺医药有限公司 | Black tea active powder and solid beverage thereof |
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| CN113249233A (en) * | 2021-06-29 | 2021-08-13 | 北京百奥茵诺生物科技有限公司 | Eurotium cristatum and microbial inoculum and application thereof |
| CN113249233B (en) * | 2021-06-29 | 2021-11-09 | 北京百奥茵诺生物科技有限公司 | Eurotium cristatum and microbial inoculum and application thereof |
| CN114431312A (en) * | 2022-01-24 | 2022-05-06 | 浙江欣诺医药有限公司 | Black tea active powder and solid beverage thereof |
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| CN107502561B (en) | 2020-08-04 |
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