CN107502450B - Mustang essential oil and application thereof - Google Patents

Mustang essential oil and application thereof Download PDF

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Publication number
CN107502450B
CN107502450B CN201710752684.4A CN201710752684A CN107502450B CN 107502450 B CN107502450 B CN 107502450B CN 201710752684 A CN201710752684 A CN 201710752684A CN 107502450 B CN107502450 B CN 107502450B
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essential oil
amur grape
amur
antibacterial
skin care
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CN107502450A (en
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吕保樱
方娜
廖娜
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Guilin paite Gaode Biotechnology Co.,Ltd.
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Guangxi University of Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a amur grape essential oil and application thereof, which are obtained by adopting the following method: soaking the amur grape in water, heating to boil, continuously boiling for 2-5 hours after collecting the first drop of volatile oil, continuously collecting the residual volatile oil, and dehydrating the collected volatile oil; wherein the Mucuna birdwoodiana refers to Mucuna woodiana collected for no more than 2 hours, and the Mucuna woodiana refers to stems, leaves or flowers of the Mucuna woodiana. The application scheme of the amur grape essential oil in preparing the antibacterial washing product and/or the skin care product is that the amur grape essential oil is used as an auxiliary raw material with antibacterial effect and is added into the antibacterial washing product and/or the skin care product. The amur grape essential oil is used as an antibacterial active ingredient for preparing antibacterial washing products and/or skin care products, has an obvious antibacterial effect, enlarges the application range of amur grapes, and increases the application value of amur grapes.

Description

Mustang essential oil and application thereof
Technical Field
The invention relates to the technical field of plant processing and application thereof, in particular to deep processing of amur grape and application thereof.
Background
Mucuna vitis, an evergreen shrub, generally up to more than ten meters, belongs to the species Mucuna vitis of the Vitaceae genus. At present, the value of the amur grape is mainly embodied in greening cities, roads, gardens and the like, and in addition, the amur grape can also be used as wood, can also be used as fruits for eating, and has narrow application range and low value. Therefore, research and development of more and higher values of the amur grape are the technical problems that the improvement of the application value of the amur grape needs to be solved urgently at present.
Disclosure of Invention
The invention provides a amur grape essential oil and application thereof, so as to solve the problems of narrow application range and low value of the existing amur grape.
The technical scheme adopted by the invention is as follows:
the amur grape essential oil is obtained by adopting the following method: soaking the amur grape in water, heating to boil, continuously boiling for 2-5 hours after the first drop of volatile oil is collected, continuously collecting the residual volatile oil, and dehydrating the collected volatile oil.
In the above technical solution, a more specific technical solution may also be: the Musca domestica is collected for no more than 2 hours.
Further, the amur grape refers to the stem or leaf or flower of amur grape.
The application scheme of the amur grape essential oil in preparing the antibacterial washing product and/or the skin care product is that the amur grape essential oil is used as an auxiliary raw material with antibacterial effect and added into the antibacterial washing product and/or the skin care product in the process of preparing the antibacterial washing product and/or the skin care product.
In the above technical solution, a more specific technical solution may also be: the bacteria are enterobacter and staphylococcus aureus.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
the amur grape essential oil is used as an antibacterial active ingredient for preparing antibacterial washing products and/or skin care products, has an obvious antibacterial effect, enlarges the application range of amur grapes, and increases the application value of amur grapes.
Detailed Description
The invention is further described in detail below with reference to specific examples:
example 1 Mustaphylos essential oil
The amur grape essential oil is obtained by adopting the following method:
weighing 500g of the stems of the amur grape, cutting into pieces, putting the pieces into a 2000ml round bottom flask, and adding 500ml of water to submerge the stems of the amur grape; then heating at constant temperature of 180 ℃, starting timing after the first drop of volatile oil is dripped out, boiling for 2h, ending distillation, stopping heating, standing the collected volatile oil for 20min, after oil-water separation, taking the upper layer liquid in the oil-water separator as crude oil, and dehydrating with anhydrous sodium sulfate to obtain the final product.
Example 2 Mustaphylos leaf essential oil
The amur grape essential oil is obtained by adopting the following method:
weighing 500g of the leaves of the amur grape, cutting into pieces, putting the pieces into a 2000ml round bottom flask, and adding 1000ml of water to submerge the leaves of the amur grape; then heating at constant temperature of 180 ℃, starting timing after the first drop of volatile oil is dripped out, boiling for 4h, ending distillation, stopping heating, standing the collected volatile oil for 20min, after oil-water separation, taking the upper layer liquid in the oil-water separator as crude oil, and dehydrating with anhydrous sodium sulfate to obtain the final product.
Example 3 Mustacia essential oil
The amur grape essential oil is obtained by adopting the following method:
weighing 500g of flowers of amur grape, cutting into pieces, putting into a 2000ml round bottom flask, and adding 500ml of water to submerge the flowers of amur grape; then heating at a constant temperature of 200 ℃, starting timing after the first drop of volatile oil is dripped out, boiling for 2h, ending distillation, stopping heating, standing the collected volatile oil for 20min, after oil-water separation, taking the upper layer liquid in an oil-water separator as crude oil, and dehydrating with anhydrous sodium sulfate to obtain the final product.
Determination of bacteriostatic Activity
Plate counting method
Respectively culturing strains to be detected (Escherichia coli) and Staphylococcus aureus subsp. Aureus) with beef extract peptone liquid culture medium, and diluting each bacterium liquid to 1x10 with turbidimeter before use8CFU·mL-1。
The method for measuring the bacteriostatic activity of the staphylococcus aureus comprises the following steps: the sterilized BP culture solution was poured into a petri dish with a diameter of 9cm, after the culture medium was solidified, 100. mu.l of each bacterial solution and essential oil (filtered through a 45 μm filter membrane) in the following table volume were sucked by a pipette and evenly coated on a plate, and each treatment was repeated three times, with distilled water as a blank and penicillin as a positive control. After the essential oil of Vitis vinifera obtained from example 1 (i.e., the essential oil of Vitis vinifera is from the stem), example 2 (i.e., the essential oil of Vitis vinifera is from the leaf), and example 3 (i.e., the essential oil of Vitis vinifera is from the flower) were sufficiently absorbed, each of them was cultured in an inverted state at 37 ℃ for 24 hours, and the number of plates was measured.
The method for measuring the bacteriostatic activity of the escherichia coli comprises the following steps: the sterilized crystal violet neutral red bile salt agar culture solution is poured into a culture dish with the diameter of 9cm, after the culture medium is solidified, 100 mu l of each bacterial solution and essential oil (passing through a 45 mu m filter membrane) with the volume according to the following table are sucked by a pipette gun and evenly coated on a flat plate, each treatment is repeated for three times, distilled water is used as a blank, and penicillin is used as a positive control. After the essential oil of Vitis vinifera obtained from example 1 (i.e., the essential oil of Vitis vinifera is from the stem), example 2 (i.e., the essential oil of Vitis vinifera is from the leaf), and example 3 (i.e., the essential oil of Vitis vinifera is from the flower) were sufficiently absorbed, each of them was cultured in an inverted state at 37 ℃ for 24 hours, and the number of plates was measured. The results are shown in table 1 below.
TABLE 1
Figure 490917DEST_PATH_IMAGE001
From the above tests and the results thereof, it can be seen that the vitis amurensis essential oil of the present invention has bacteriostatic effect, especially on escherichia coli and staphylococcus aureus; although the bacteriostatic effect of the antibacterial agent is not equal to or higher than that of antibiotic (penicillin), the bacteriostatic effect of the antibacterial agent is still significant.
The method comprises the steps of respectively adding 800 mu L of essential oil extracted in example 1 (namely, the amur grape essential oil is from stems), example 2 (namely, the amur grape essential oil is from flowers) and example 3 (namely, the amur grape essential oil is from flowers) as auxiliary materials into 200g of non-bacteriostatic-effect liquid soap, taking the non-bacteriostatic-effect liquid soap as blank comparison in blank, taking 5g of the non-bacteriostatic-effect liquid soap as a sampling unit every 120min, sampling 5g of the non-bacteriostatic-effect liquid soap before bacteriostatic experiments (adding or not adding the field essential oil auxiliary materials after carrying out high-temperature sterilization treatment on the liquid soap, placing the liquid soap in open air, carrying out parallel sampling for three times according to different sampling times, carrying out bacteriostatic activity experiments on average values, counting according to the bacteriostatic activity represented by staphylococcus aureus (a plate counting method), and counting. The results of the experiment are shown in table 2 below.
TABLE 2
Figure 242973DEST_PATH_IMAGE002
Therefore, the amur grape essential oil has a good antibacterial effect in antibacterial washing products, and has an application value in washing products.
800 muL of essential oil extracted in example 1 (namely, the amur grape essential oil is from stems), example 2 (namely, the amur grape essential oil is from leaves) and example 3 (namely, the amur grape essential oil is from flowers) is used as an auxiliary material and is respectively added into 200g of the hand cream with no bacteriostatic effect, blank is compared with the hand cream with no bacteriostatic effect, every 120min is used as a sampling unit, 5g of the hand cream with no bacteriostatic effect is sampled each time (the hand cream with no bacteriostatic effect is subjected to high-temperature sterilization treatment before bacteriostatic experiments, the hand cream with no bacteriostatic effect is added or not subjected to field essential oil auxiliary materials and is placed in open air, samples are parallelly sampled for three times according to different sampling times, bacteriostatic activity experiments are carried out according to an average value, staphylococcus aureus is used as a representative according to the bacteriostatic activity (a plate counting method), and the experiment results of the.
TABLE 3
Figure 721228DEST_PATH_IMAGE003
Therefore, the amur grape essential oil has a good antibacterial effect in antibacterial skin care products, and has an application value in skin care products.

Claims (2)

1. The application of the amur grape essential oil in preparing bacteriostatic washing products or skin care products is characterized in that: in the process of preparing the antibacterial washing product and/or the skin care product, the amur grape essential oil is used as an auxiliary raw material with antibacterial effect and is added into the antibacterial washing product and/or the skin care product;
the bacteria are escherichia coli and staphylococcus aureus;
the amur grape essential oil is obtained by adopting the following method:
soaking the amur grape in water, heating to boil, continuously boiling for 2-5 hours after the first drop of volatile oil is collected, continuously collecting the residual volatile oil, and dehydrating the collected volatile oil;
the amur grape refers to the stem or leaf or flower of amur grape.
2. The use of essential oil of Vitis vinifera as claimed in claim 1 in the preparation of bacteriostatic washing products or skin care products, wherein: the Musca domestica is collected for no more than 2 hours.
CN201710752684.4A 2017-08-28 2017-08-28 Mustang essential oil and application thereof Active CN107502450B (en)

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CN108776222B (en) * 2018-06-14 2021-05-04 吉林省农业科学院 Method for detecting antibacterial activity of princess lingmycin and application
CN111592936A (en) * 2020-05-15 2020-08-28 广西科技大学 Acacia cercis bark essential oil and application thereof

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JPS55164606A (en) * 1979-06-12 1980-12-22 Kyowa Gas Chem Ind Co Ltd Novel agent for controlling microorganism
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JP3547058B2 (en) * 1995-03-31 2004-07-28 株式会社パル Antibacterial wooden building materials
CN102618386A (en) * 2012-03-14 2012-08-01 东南大学 Method for preparing chenopodium vulvaria volatile oil and application of chenopodium vulvaria volatile oil
CN102687634A (en) * 2012-05-21 2012-09-26 中国科学院植物研究所 Method for cultivating rosemary

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Effective date of registration: 20210906

Address after: 541004 floor 3, building 2, building 7, No. 39, Longyin Road, Qixing District, Guilin City, Guangxi Zhuang Autonomous Region

Patentee after: Guilin paite Gaode Biotechnology Co.,Ltd.

Address before: 545006 268 East Ring Road, Liuzhou, the Guangxi Zhuang Autonomous Region

Patentee before: GUANGXI University OF SCIENCE AND TECHNOLOGY