CN107501433A - A kind of polygonum flaccidum polyoses extract and preparation method thereof and purposes - Google Patents
A kind of polygonum flaccidum polyoses extract and preparation method thereof and purposes Download PDFInfo
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- CN107501433A CN107501433A CN201710849036.0A CN201710849036A CN107501433A CN 107501433 A CN107501433 A CN 107501433A CN 201710849036 A CN201710849036 A CN 201710849036A CN 107501433 A CN107501433 A CN 107501433A
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- polygonum flaccidum
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- polyoses extract
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The present invention relates to plant polyose technical field, is a kind of polygonum flaccidum Polyose extraction and preparation method thereof, and polygonum flaccidum polysaccharide has the function that good anti-inflammatory, analgesia, regulation are immune.The polygonum flaccidum polyoses extract is obtained by following preparation methods：The first step, degreasing；Second step, water extraction；3rd step, take off albumen；4th step, alcohol precipitation；5th step, dry.The invention provides a kind of method that polysaccharide to polygonum flaccidum is extracted, and compensate for the defects of polygonum flaccidum polysaccharide and preparation method thereof still belongs to blank in plant polyose field, and for the later stage, the utilization of polygonum flaccidum polysaccharide provide foundation.
Description
Technical field
The present invention relates to the polyoses extract for extracting to obtain from plant polygonum flaccidum, its extracting method, contain the Polyose extraction
The pharmaceutical composition of thing and the polyoses extract or its pharmaceutical composition are as anti-inflammatory, analgesia, regulation immunology medicine
Purposes.
Background technology
Polygonum flaccidum, annual herb plant, upright or bottom are thrown oneself on the ground, and have a fibrous root, and leaf is in oval or lanceolar, alternate.It is peppery
Knotweed is mainly distributed on south east asia, the place for the humidity that grows on the water's edge, is distributed in China various regions, and Zhejiang the whole province is distributed
It is relatively broad, wild resource enriches.Polygonum flaccidum is included in food by polygonum flaccidum herb acrid flavour, many countries such as China and Southeast Asia
In additive, it can be used for increasing the flavor of food.The traditional medicinal material of polygonum flaccidum or China, to its pharmacology in many ancient medical books
Function had record, had the effect of stagnant dampness elimination, row, wind-dispelling, detumescence.Clinical research finds that polygonum flaccidum has antibacterial, anti-inflammatory, resisted
Viral, anti-oxidant, inhibiting cancer, pain easing and hemostasis and other effects, flavone compound are its main bioactive ingredients.
The correlative study of its polysaccharide is had no at present.
At present, plant polyose extracting method has sour formulation, alkaline extraction, enzymatic isolation method and ultrasonic extraction etc., by using acid
Formulation, alkaline extraction, enzymatic isolation method and ultrasonic extraction extract to polygonum flaccidum polysaccharide, and discovery has following defect：Sour formulation exists
The fracture of glycosidic bond in polygonum flaccidum polysaccharide can be caused under acid condition, and acid can cause to corrode to container, therefore it is more in extraction polygonum flaccidum
, it is necessary to be carried out in special installation when sugared, production cost is thus improved indirectly；Alkaline extraction extraction polygonum flaccidum polysaccharide solution has
Denseer alkali taste, solution colour are in yellow, can so influence the flavor and color and luster of finished product；Enzymatic isolation method cost is higher；Ultrasound carries
It is more abundant to follow the example of the extraction when extracting polygonum flaccidum polysaccharide, but ultrasonic useful effect region is an annular, if the diameter of extractor
Too big, center will form ultrasonic clear area, and ultrasonic cell is easily broken, and make that extraction impurity is more, viscosity is excessive, be not easy
Filter, therefore the extraction method should not be mass produced.
The content of the invention
The invention provides a kind of polygonum flaccidum polyoses extract and preparation method thereof, can effectively solve sour formulation, alkaline extraction, enzyme
High production cost, poor product quality be present and should not carry out in solution and ultrasonic extraction when carrying out the extraction of polygonum flaccidum polysaccharide
The problem of large-scale production.
The polygonum flaccidum polyoses extract of the present invention can use individually or with pharmaceutical compositions, and its administering mode can be according to tool
Depending on body situation, can by oral, non-bowel or it is local be administered, form of administration can be such as tablet, capsule, paste, patch,
Injection etc..
The technical scheme is that realized by following measures：A kind of polygonum flaccidum polyoses extract, as follows
Obtain：The first step, the ethanol extraction of polygonum flaccidum medicinal material 95% is one or many, ethanol is reclaimed after collecting extract solution, volatilizes polygonum flaccidum
Middle residual ethanol；Second step, by polygonum flaccidum and distilled water in mass ratio 1:30 to 1:After 50 mix, temperature be 85 DEG C extremely
Water extraction 4 hours to 6 hours under the conditions of 100 DEG C, the mixed solution after water extraction is filtered and collects filtrate, is concentrated the filtrate to
Quality is 25 times to 35 times of polygonum flaccidum quality and obtains concentrate；3rd step, concentrate and sevage reagents are pressed into volume 1:1 to 1:
After 2 mix, vibrate and carried out within 30 minutes to 50 minutes at de- albumen under the conditions of rotating speed is 120r/min to 150r/min
Reason, after vibration terminates, mixed solution is subjected to stratification, stands to after bed boundary is clear, discards upper strata sevage layers
After leave lower floor's feed liquid layer；4th step, into feed liquid layer by volume 1:4 to 1:6, which add alcohol precipitation solvent, carries out alcohol precipitation, and alcohol is sink to
In solution again without brown floccule generation i.e. obtain alcohol precipitation mixed solution, by alcohol precipitation mixed solution rotating speed be 3000r/min extremely
Centrifugation 5 minutes to 6 minutes is carried out under the conditions of 4500r/min, alcohol precipitation mixed solution is layered to obtain polygonum flaccidum polysaccharide layers and alcohol after centrifugation
Heavy solvent layer, then carry out separating and collecting polygonum flaccidum polysaccharide layers obtaining polygonum flaccidum polysaccharide solution；5th step, polygonum flaccidum polysaccharide solution is dried
Polygonum flaccidum polyoses extract is obtained to constant weight.
Here is the further optimization or improvement to foregoing invention technical scheme：
In the above-mentioned first step, the powdered of 60 mesh sieve to 80 mesh sieve can be crossed by being first crushed to polygonum flaccidum.
In above-mentioned 3rd step, sevage reagents are chloroform and n-butanol by volume 3:1 to 5:1 mixes
Mixed solution.
In above-mentioned 4th step, alcohol precipitation solvent is absolute ethyl alcohol or mass concentration is 95% ethanol water.
In above-mentioned 5th step, dried under the conditions of being 60 DEG C in temperature by polygonum flaccidum polysaccharide solution to constant weight and obtain polygonum flaccidum polysaccharide
Extract.
The present invention has higher yield compared to currently used plant polyose extracting method, meanwhile, present invention extraction is peppery
Knotweed polysaccharide is not required to special installation, and production technology cost is low, safety, is adapted to industrialized production；And according to embodiments of the present invention
The polygonum flaccidum polysaccharide of method extraction under dissolved state color and luster clarification free from admixture, when being solid-state be in brown color.
Embodiment
The present invention is not limited by following embodiments, can technique according to the invention scheme and actual conditions it is specific to determine
Embodiment.
With reference to embodiment, the invention will be further described：
Embodiment 1, the polygonum flaccidum polyoses extract are obtained by following preparation methods：The first step, the ethanol of polygonum flaccidum medicinal material 95% is carried
Take it is one or many, collect extract solution after reclaim ethanol, volatilize residual ethanol in polygonum flaccidum；Second step, polygonum flaccidum and distilled water are pressed
Mass ratio 1:30 to 1:After 50 mix, water extraction 4 hours to 6 hours under the conditions of being 85 DEG C to 100 DEG C in temperature, by water extraction
Mixed solution afterwards is filtered and collects filtrate, is concentrated the filtrate to 25 times to 35 times that quality is polygonum flaccidum quality and is concentrated
Liquid；3rd step, by concentrate and sevage reagents by volume 1:1 to 1:After 2 mix, rotating speed be 120r/min extremely
Vibrated under the conditions of 150r/min and carry out within 30 minutes to 50 minutes de- albumen processing, after vibration terminates, mixed solution is stood
Layering, stand to after bed boundary is clear, lower floor's feed liquid layer is left after discarding upper strata sevage layers；4th step, to feed liquid layer
In by volume 1:4 to 1:6, which add alcohol precipitation solvent, carries out alcohol precipitation, and alcohol is sink in solution obtains alcohol without the generation of brown floccule again
Heavy mixed solution, alcohol precipitation mixed solution is subjected to centrifugation 5 minutes to 6 points under the conditions of rotating speed is 3000r/min to 4500r/min
Clock, alcohol precipitation mixed solution is layered to obtain polygonum flaccidum polysaccharide layers and alcohol precipitation solvent layer after centrifugation, then carries out separating and collecting polygonum flaccidum polysaccharide
Layer obtains polygonum flaccidum polysaccharide solution；5th step, polygonum flaccidum polysaccharide solution drying to constant weight is obtained into polygonum flaccidum polyoses extract.
Embodiment 2,
It is with the difference of above-described embodiment, the powdered of 60 mesh sieve to 80 mesh sieve can be crossed by being first crushed to polygonum flaccidum.
Embodiment 3, as the preferred of above-described embodiment, in the 3rd step, sevage reagents are that chloroform and n-butanol are pressed
Volume ratio 3:1 to 5:1 mixed solution mixed.
Embodiment 4, as the preferred of above-described embodiment, in the 4th step, alcohol precipitation solvent is absolute ethyl alcohol or mass concentration is
95% ethanol water.
Embodiment 5, in temperature it is 60 DEG C of bars by polygonum flaccidum polysaccharide solution in the 5th step as the preferred of above-described embodiment
Dried under part to constant weight and obtain polygonum flaccidum polyoses extract.
The present invention is water extraction and alcohol precipitation method, the sour formulation conventional compared to current extraction plant polyose, alkaline extraction, enzymatic isolation method with
And ultrasonic extraction, the yield average out to 1.51% of the polygonum flaccidum polysaccharide of method extraction according to embodiments of the present invention, and use existing
The yield average value for the polygonum flaccidum polysaccharide that conventional plant polyose extracting method is extracted is 0.84%；Meanwhile water of the invention
Carry when alcohol deposition method is extracted in extraction polygonum flaccidum polysaccharide and be not required to special installation, production technology cost is low, safety, is adapted to industrialized production
；And the polygonum flaccidum polysaccharide of method extraction according to embodiments of the present invention under dissolved state color and luster clarification free from admixture, be in when being solid-state
Brown color.
Claims (6)
1. a kind of polygonum flaccidum polyoses extract, it is characterised in that obtain as follows：The first step, by the ethanol of polygonum flaccidum medicinal material 95%
Extract it is one or many, collect extract solution after reclaim ethanol, volatilize residual ethanol in polygonum flaccidum；Second step, by polygonum flaccidum and distilled water
In mass ratio 1:30 to 1:After 50 mix, water extraction 4 hours to 6 hours under the conditions of being 85 DEG C to 100 DEG C in temperature, by water
Mixed solution after carrying is filtered and collects filtrate, concentrate the filtrate to quality be 25 times to 35 times of polygonum flaccidum quality obtain it is dense
Contracting liquid；3rd step, by concentrate and sevage reagents by volume 1:1 to 1:It is 120r/min in rotating speed after 2 mix
Vibrated under the conditions of to 150r/min and carry out within 30 minutes to 50 minutes de- albumen processing, after vibration terminates, mixed solution carried out quiet
Layering is put, stands to after bed boundary is clear, lower floor's feed liquid layer is left after discarding upper strata sevage layers；4th step, to feed liquid
By volume 1 in layer:4 to 1:6, which add alcohol precipitation solvent, carries out alcohol precipitation, and alcohol is sink in solution to be obtained without the generation of brown floccule again
Alcohol precipitation mixed solution, alcohol precipitation mixed solution is subjected to centrifugation 5 minutes to 6 under the conditions of rotating speed is 3000r/min to 4500r/min
Minute, alcohol precipitation mixed solution is layered to obtain polygonum flaccidum polysaccharide layers and alcohol precipitation solvent layer after centrifugation, then carries out separating and collecting polygonum flaccidum more
Sugar layer obtains polygonum flaccidum polysaccharide solution；5th step, polygonum flaccidum polysaccharide solution drying to constant weight is obtained into polygonum flaccidum polyoses extract.
2. polygonum flaccidum polyoses extract according to claim 1, it is characterised in that in the first step, being first crushed to polygonum flaccidum can mistake
60 mesh sieve are powdered to 80 mesh sieve.
3. the polygonum flaccidum polyoses extract according to right 1, it is characterised in that in the 3rd step, sevage reagents be chloroform and
N-butanol by volume 3:1 to 5:1 mixed solution mixed.
4. polygonum flaccidum polyoses extract according to claim 1, its feature in the 4th step, alcohol precipitation solvent be absolute ethyl alcohol or
Mass concentration is 95% ethanol water.
5. polygonum flaccidum polyoses extract according to claim 1, it is characterised in that in the 5th step, by polygonum flaccidum polysaccharide solution in temperature
Spend and polygonum flaccidum polyoses extract is obtained to constant weight for drying under the conditions of 60 DEG C.
6. polygonum flaccidum polyoses extract according to claim 1 is being prepared for anti-inflammatory, analgesia, the medicine for adjusting immunology
In purposes.
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| CN201710849036.0A CN107501433A (en) | 2017-09-20 | 2017-09-20 | A kind of polygonum flaccidum polyoses extract and preparation method thereof and purposes |
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| CN201710849036.0A CN107501433A (en) | 2017-09-20 | 2017-09-20 | A kind of polygonum flaccidum polyoses extract and preparation method thereof and purposes |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480419A (en) * | 2007-11-23 | 2009-07-15 | 中国人民解放军军事医学科学院毒物药物研究所 | Polysaccharide extract of Periploca forrestii Schltr, pharmaceutical composition containing the same as well as preparation method and application thereof |
| CN103483461A (en) * | 2013-08-28 | 2014-01-01 | 新疆医科大学 | Ziziphora clinopodioides polysaccharide extract and preparation method thereof |
-
2017
- 2017-09-20 CN CN201710849036.0A patent/CN107501433A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480419A (en) * | 2007-11-23 | 2009-07-15 | 中国人民解放军军事医学科学院毒物药物研究所 | Polysaccharide extract of Periploca forrestii Schltr, pharmaceutical composition containing the same as well as preparation method and application thereof |
| CN103483461A (en) * | 2013-08-28 | 2014-01-01 | 新疆医科大学 | Ziziphora clinopodioides polysaccharide extract and preparation method thereof |
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