CN107496727A - A kind of extracting method of flavones - Google Patents
A kind of extracting method of flavones Download PDFInfo
- Publication number
- CN107496727A CN107496727A CN201710679231.3A CN201710679231A CN107496727A CN 107496727 A CN107496727 A CN 107496727A CN 201710679231 A CN201710679231 A CN 201710679231A CN 107496727 A CN107496727 A CN 107496727A
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- CN
- China
- Prior art keywords
- bamboo
- flavones
- leaf
- extract
- reflux
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracting method of flavones, comprise the steps of：（1）Feedstock treating：The fresh leaf of bamboo is gathered, fixing drying is carried out to the leaf of bamboo with micro-wave oven, the leaf of bamboo after drying is ground into less than 40 mesh powders by pulverizer；（2）Extracting flavonoids：Above-mentioned 180 ~ 200 parts of bamboo sample powder is weighed in reflux, then adds 130 ~ 150 part of 60% ethanol solution, 30min is soaked, reflux is placed in water-bath, circumfluence distillation 1h, room temperature is subsequently cooled to, filters to get filtrate, produces flavones crude extract；（3）Extraction：60 ~ 70 parts of petroleum ethers are added into flavones crude extract, while add extractant, is then extracted, removes heavy liquid, then add the petroleum ether of equal quality part again into sinking liquid, repeats 2 ~ 3 times, finally extract solution is sealed into refrigerator；（4）Concentration：Stripped extract solution is placed in Rotary Evaporators, dried residue is flavones primary extract.The present invention re-uses the leaf of bamboo, economizes on resources, has good market prospects.
Description
Technical field
The present invention relates to a kind of extracting method of flavones, applied to Extracting Technique of Flavonoids field.
Background technology
Flavonoid substances are a kind of natural plant compositions, have significant physiology and pharmacological activity, have obvious antiulcer,
The bioactivity such as spasmolysis, antibacterial, anti-inflammatory, reducing blood lipid, analgesia and physiological activity, in daily life, people can be by more
Food grape, onion, red wine etc. is drunk to obtain flavones, Recent study is found, contains Flavonoid substances in the leaf of bamboo, and bamboo is adding
During work, the leaf of bamboo is always thrown aside, and causes significant wastage, if therefore flavones can be extracted from the leaf of bamboo, there is good market
Prospect.
The content of the invention
It is above-mentioned to solve the problems, such as, the invention discloses a kind of extracting method of flavones, comprise the steps of：
（1）Feedstock treating：The fresh leaf of bamboo is gathered, fixing drying is carried out to the leaf of bamboo with micro-wave oven, the leaf of bamboo after drying passes through powder
Broken machine is ground into less than 40 mesh powders, after being sealed by hermetic bag, is placed in refrigerator and is stored for future use；
（2）Extracting flavonoids：Above-mentioned 180 ~ 200 parts of bamboo sample powder is weighed in reflux, then adds 130 ~ 150 part of 60% second
Alcoholic solution, 30min is soaked, 75 DEG C is heated to by water-bath, reflux is placed in water-bath, circumfluence distillation 1h, so
After be cooled to room temperature, filter to get filtrate, produce flavones crude extract；
（3）Extraction：60 ~ 70 parts of petroleum ethers are added into flavones crude extract, while add extractant, is then extracted, removed
Heavy liquid, then add the petroleum ether of equal quality part again into sinking liquid, repeat 2 ~ 3 times, be finally sealed extract solution
Into refrigerator；
（4）Concentration：Stripped extract solution is placed in Rotary Evaporators, dried residue is flavones primary extract.
Step（3）Described in extractant be water saturated n-butanol, the mass ratio of itself and flavones crude extract is 1:1.
Using the technical program, the beneficial effects of the invention are as follows：The situation for avoiding the leaf of bamboo from throwing aside occurs, and economizes on resources, together
Shi Caiyong this laws extract flavones from the leaf of bamboo, and total content and purity are obviously improved.
Embodiment
The invention discloses a kind of extracting method of flavones, comprise the following steps that：
（1）Feedstock treating：The fresh leaf of bamboo is gathered, fixing drying is carried out to the leaf of bamboo with micro-wave oven, the leaf of bamboo after drying passes through powder
Broken machine is ground into less than 40 mesh powders, after being sealed by hermetic bag, is placed in refrigerator and is stored for future use；
（2）Extracting flavonoids：Above-mentioned 180 ~ 200 parts of bamboo sample powder is weighed in reflux, then adds 130 ~ 150 part of 60% second
Alcoholic solution, 30min is soaked, 75 DEG C is heated to by water-bath, reflux is placed in water-bath, circumfluence distillation 1h, so
After be cooled to room temperature, filter to get filtrate, produce flavones crude extract；
（3）Extraction：60 ~ 70 parts of petroleum ethers are added into flavones crude extract, while add extractant, is then extracted, removed
Heavy liquid, then add the petroleum ether of equal quality part again into sinking liquid, repeat 2 ~ 3 times, be finally sealed extract solution
Into refrigerator；
（4）Concentration：Stripped extract solution is placed in Rotary Evaporators, dried residue is flavones primary extract.
Finally it should be noted that：Above example only not limits technology described in the invention to illustrate the present invention
Scheme；Therefore, although this specification with reference to above-mentioned each embodiment to present invention has been detailed description, this
Field it is to be appreciated by one skilled in the art that still can be modified to the present invention or equivalent substitution；And all do not depart from this
The technical scheme of the spirit and scope of invention and its improvement, it all should cover in scope of the presently claimed invention.
Claims (2)
1. a kind of extracting method of flavones, it is characterised in that comprise the steps of：
（1）Feedstock treating：The fresh leaf of bamboo is gathered, fixing drying is carried out to the leaf of bamboo with micro-wave oven, the leaf of bamboo after drying passes through powder
Broken machine is ground into less than 40 mesh powders, after being sealed by hermetic bag, is placed in refrigerator and is stored for future use；
（2）Extracting flavonoids：Above-mentioned 180 ~ 200 parts of bamboo sample powder is weighed in reflux, then adds 130 ~ 150 part of 60% second
Alcoholic solution, 30min is soaked, 75 DEG C is heated to by water-bath, reflux is placed in water-bath, circumfluence distillation 1h, so
After be cooled to room temperature, filter to get filtrate, produce flavones crude extract；
（3）Extraction：60 ~ 70 parts of petroleum ethers are added into flavones crude extract, while add extractant, is then extracted, removed
Heavy liquid, then add the petroleum ether of equal quality part again into sinking liquid, repeat 2 ~ 3 times, be finally sealed extract solution
Into refrigerator；
（4）Concentration：Stripped extract solution is placed in Rotary Evaporators, dried residue is flavones primary extract.
2. according to a kind of extracting method of flavones described in claim 1, it is characterised in that：Step（3）Described in extraction
Agent is water saturated n-butanol, and the mass ratio of itself and flavones crude extract is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710679231.3A CN107496727A (en) | 2017-08-10 | 2017-08-10 | A kind of extracting method of flavones |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710679231.3A CN107496727A (en) | 2017-08-10 | 2017-08-10 | A kind of extracting method of flavones |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107496727A true CN107496727A (en) | 2017-12-22 |
Family
ID=60690592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710679231.3A Pending CN107496727A (en) | 2017-08-10 | 2017-08-10 | A kind of extracting method of flavones |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107496727A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104557893A (en) * | 2014-12-19 | 2015-04-29 | 国际竹藤中心 | Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves |
| CN106109367A (en) * | 2016-08-31 | 2016-11-16 | 王娟 | A kind of toothpaste with antibacterial action containing bamboo leaf flavone and preparation method thereof |
-
2017
- 2017-08-10 CN CN201710679231.3A patent/CN107496727A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104557893A (en) * | 2014-12-19 | 2015-04-29 | 国际竹藤中心 | Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves |
| CN106109367A (en) * | 2016-08-31 | 2016-11-16 | 王娟 | A kind of toothpaste with antibacterial action containing bamboo leaf flavone and preparation method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171222 |
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| RJ01 | Rejection of invention patent application after publication |