CN107493969A - A kind of high-efficient culture method of cordyceps sinensis - Google Patents
A kind of high-efficient culture method of cordyceps sinensis Download PDFInfo
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- CN107493969A CN107493969A CN201710778745.4A CN201710778745A CN107493969A CN 107493969 A CN107493969 A CN 107493969A CN 201710778745 A CN201710778745 A CN 201710778745A CN 107493969 A CN107493969 A CN 107493969A
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- Prior art keywords
- culture medium
- nutrient solution
- strain
- cordyceps sinensis
- culture method
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
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Abstract
The invention discloses a kind of high-efficient culture method of cordyceps sinensis, including：（1）Prepare culture medium：The raw material prepared needed for culture medium of Cordyceps militaris is mixed and stirred, is configured to culture medium；（2）Handle culture medium：Heat sterilization processing is carried out to culture medium, culture medium cooled down after the completion of heating；（3）Handle nutrient solution：Heat sterilization processing is carried out to the nutrient solution for strain fermentation, nutrient solution cooled down after the completion of heating；（4）Add strain：After the completion of cooling, strain is added into nutrient solution；（5）Strain fermentation：Compressed air after filtering is continually fed into nutrient solution and carries out strain fermentation, forms bacterium solution；（6）Inoculation：Bacterium solution is passed through in culture medium, is placed in carrying out bacterium germination processing in sterile darkroom, until growing cordyceps militaris sporocarp.A kind of high-efficient culture method of cordyceps sinensis of the present invention, simple to operate and yield are high.
Description
Technical field
The present invention relates to cordyceps sinensis to cultivate field, and in particular to a kind of high-efficient culture method of cordyceps sinensis.
Background technology
Existing cordyceps sinensis is cultivated, when cultivating live body strain, frequently with shaking table come the shaking culture produced, this method culture
Live body strain, workload is big, the time is long, complex operation, and yield is very low；Meanwhile after the completion of live body Spawn incubation, cultivate worm
, it is necessary to which strain is injected in silkworm chrysalis body with syringe when careless, waste time and energy and yield poorly.
The content of the invention
It is an object of the invention to provide the high-efficient culture method of cordyceps sinensis a kind of simple to operate and high yield.
To reach above-mentioned purpose, the technical solution adopted by the present invention is：
A kind of high-efficient culture method of cordyceps sinensis, including：
（1）Prepare culture medium：The raw material prepared needed for culture medium of Cordyceps militaris is mixed and stirred, is configured to the culture
Base；
（2）Handle culture medium：Heat sterilization processing is carried out to the culture medium, the culture medium carried out after the completion of heating cold
But；
（3）Handle nutrient solution：Heat sterilization processing is carried out to the nutrient solution for strain fermentation, to the nutrition after the completion of heating
Liquid is cooled down；
（4）Add strain：After the completion of cooling, strain is added into the nutrient solution；
（5）Strain fermentation：Compressed air after filtering is continually fed into the nutrient solution and carries out strain fermentation, forms bacterium solution；
（6）Inoculation：The bacterium solution is passed through in the culture medium, is placed in carrying out bacterium germination processing in sterile darkroom, until growing
Cordyceps militaris sporocarp.
Preferably, in the step（1）In, the mixing time of the culture medium is 20-25 minutes.
Preferably, in the step（2）In, the design temperature of the culture medium heating is 121 DEG C -123 DEG C, and cooling is set
Constant temperature degree is 20 DEG C -25 DEG C.
Preferably, in the step（3）In, the design temperature of the nutrient solution heating is 121 DEG C -123 DEG C, and cooling is set
Constant temperature degree is 20 DEG C -25 DEG C.
Preferably, in the step（3）In, an inner pressurd vessel for being used to place the nutrient solution is set, in the content
An outer container is arranged on device, gap is set between the inner pressurd vessel outer surface and the outer container inner surface, between described
Cooling liquid is passed through in gap and is exchanged heat with the nutrient solution.
Preferably, in the step（4）In, when the strain is put into the nutrient solution, it is being put into the strain
Porch ring set a circle combustible medium, when being put into the strain, light the combustible medium, form flame sealing ring.
Preferably, in the step（5）In, the filtering carried out to the compressed air is divided into what filtering accuracy increased successively
Three-stage filtration.
Preferably, in the step（5）In, the compressed air that is continually fed into after the filtering sets duration of ventilation as 48
Hour.
Preferably, in the step（6）In, the sterile darkroom sets room temperature as 20 DEG C -25 DEG C.
Preferably, in the step（6）In, the time of the bacterium germination processing is 8-10 days.
Due to the utilization of above-mentioned technical proposal, the present invention has following advantages compared with prior art：One kind of the present invention
The high-efficient culture method of cordyceps sinensis, simple to operate and yield are high.
Embodiment
Technical scheme is further elaborated with reference to specific embodiment.
A kind of high-efficient culture method of cordyceps sinensis, including：
（1）Prepare culture medium：The raw material prepared needed for culture medium of Cordyceps militaris is mixed and stirred, is configured to culture medium.
Herein, by setting a hollow cavity for being used to place culture medium, the original needed for culture medium of Cordyceps militaris will be prepared
Material, which mixes, to stir, and mixing time is set as 20-25 minutes.
（2）Handle culture medium：Heat sterilization processing is carried out to culture medium, culture medium cooled down after the completion of heating.
Herein, the hollow cavity for placing culture medium is put into boiler and carries out high-temperature heating sterilization treatment, it is right to have heated
It is cooled down.The design temperature of culture medium heating is 121 DEG C -123 DEG C, and the design temperature of cooling is 20 DEG C -25 DEG C.
（3）Handle nutrient solution：Heat sterilization processing is carried out to the nutrient solution for strain fermentation, to nutrition after the completion of heating
Liquid is cooled down.
Herein, a hollow container for being used to place nutrient solution is set, high-temperature steam is passed through into the hollow container
Nutrient solution is heated, to kill the microorganism and bacterium in nutrient solution, suitable growing environment is created for strain.Meanwhile
Design temperature is 121 DEG C, stops heating when being heated to 121 DEG C of setting.
Herein, an inner pressurd vessel for being used to place nutrient solution is set, i.e., above-mentioned hollow container, one is arranged on inner pressurd vessel
Individual outer container, gap is set between interior container outer surface and outer container inner surface, be passed through into gap cooling liquid and with battalion
Nutrient solution is exchanged heat.Wherein, the design temperature of Solution cooling is 20 DEG C -25 DEG C.Setting is cooled to when measuring solution temperature
23 DEG C when, stopping be passed through cooling liquid.Meanwhile external environment condition is also controlled at 20 DEG C -25 DEG C, it is ensured that solution temperature will not
Change.
（4）Add strain：After the completion of cooling, strain is added into nutrient solution.When strain is put into nutrient solution, putting
The porch ring for entering strain sets a circle combustible medium, when being put into strain, lights combustible medium, forms flame sealing ring.
In the present embodiment, offered on hollow container and enter bacterium mouth for be put into strain, enter bacterium mouth be put into strain
Ring sets a circle combustible medium, and combustible medium is alcohol, when adding strain into container, lights alcohol, alcohol burn forms fire
Flame sealing ring, it is ensured that the bacterium for not having other influences strain fermentation when adding strain enters, and improves strain fermentation success rate.
（5）Strain fermentation：Compressed air after filtering is continually fed into nutrient solution and carries out strain fermentation, forms bacterium solution.
Herein, the filtering carried out to compressed air is divided into the three-stage filtration that filtering accuracy increases successively, and filter core model is followed successively by E5-
20、E7-20、E9-20.The compressed air being continually fed into after filtering, set duration of ventilation as 48 hours, in 48 hours, continued
The compressed air being constantly passed through after filtering, strain constantly ferment in nutrient solution.
（6）Inoculation：Bacterium solution is passed through in culture medium, is placed in carrying out bacterium germination processing in sterile darkroom, until growing cordyceps sinensis
Fructification.The bacterium solution intermediary for completion of fermenting is passed through in the culture medium cooled down, sterile darkroom is put into and is cultivated.Sterile darkroom
Set room temperature as 20 DEG C -25 DEG C.Herein, room temperature is 23 DEG C.The time of bacterium germination processing is 8-10 days.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and be carried out, and it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, it should all cover within the scope of the present invention.
Claims (10)
1. a kind of high-efficient culture method of cordyceps sinensis, it is characterised in that：Including：
（1）Prepare culture medium：The raw material prepared needed for culture medium of Cordyceps militaris is mixed and stirred, is configured to the culture
Base；
（2）Handle culture medium：Heat sterilization processing is carried out to the culture medium, the culture medium carried out after the completion of heating cold
But；
（3）Handle nutrient solution：Heat sterilization processing is carried out to the nutrient solution for strain fermentation, to the nutrition after the completion of heating
Liquid is cooled down；
（4）Add strain：After the completion of cooling, strain is added into the nutrient solution；
（5）Strain fermentation：Compressed air after filtering is continually fed into the nutrient solution and carries out strain fermentation, forms bacterium solution；
（6）Inoculation：The bacterium solution is passed through in the culture medium, is placed in carrying out bacterium germination processing in sterile darkroom, until growing
Cordyceps militaris sporocarp.
A kind of 2. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（1）In, institute
The mixing time for stating culture medium is 20-25 minutes.
A kind of 3. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（2）In, institute
The design temperature for stating culture medium heating is 121 DEG C -123 DEG C, and the design temperature of cooling is 20 DEG C -25 DEG C.
A kind of 4. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（3）In, institute
The design temperature for stating nutrient solution heating is 121 DEG C -123 DEG C, and the design temperature of cooling is 20 DEG C -25 DEG C.
A kind of 5. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（3）In, if
An inner pressurd vessel for being used to place the nutrient solution is put, an outer container is arranged on the inner pressurd vessel, outside the inner pressurd vessel
Gap is set between surface and the outer container inner surface, cooling liquid is passed through into the gap and is carried out with the nutrient solution
Heat exchange.
A kind of 6. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（4）In,
When the strain is put into the nutrient solution, a circle combustible medium is set in the porch ring for being put into the strain, is put into described
During strain, the combustible medium is lighted, forms flame sealing ring.
A kind of 7. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（5）In, it is right
The filtering that the compressed air is carried out is divided into the three-stage filtration that filtering accuracy increases successively.
A kind of 8. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（5）In, hold
It is continuous to be passed through the compressed air after the filtering, duration of ventilation is set as 48 hours.
A kind of 9. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（6）In, institute
That states sterile darkroom sets room temperature as 20 DEG C -25 DEG C.
A kind of 10. high-efficient culture method of cordyceps sinensis according to claim 1, it is characterised in that：In the step（6）In,
The time of the bacterium germination processing is 8-10 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710778745.4A CN107493969A (en) | 2017-09-01 | 2017-09-01 | A kind of high-efficient culture method of cordyceps sinensis |
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| CN201710778745.4A CN107493969A (en) | 2017-09-01 | 2017-09-01 | A kind of high-efficient culture method of cordyceps sinensis |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN103548577A (en) * | 2013-11-18 | 2014-02-05 | 连云港国鑫食用菌成套设备有限公司 | System and method for producing and culturing liquid spawns of edible fungus |
-
2017
- 2017-09-01 CN CN201710778745.4A patent/CN107493969A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN103548577A (en) * | 2013-11-18 | 2014-02-05 | 连云港国鑫食用菌成套设备有限公司 | System and method for producing and culturing liquid spawns of edible fungus |
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Application publication date: 20171222 |