CN107460121A - A kind of PCR reaction systems are prepared and sampling device and full-automatic PCR instrument - Google Patents
A kind of PCR reaction systems are prepared and sampling device and full-automatic PCR instrument Download PDFInfo
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- CN107460121A CN107460121A CN201710603421.7A CN201710603421A CN107460121A CN 107460121 A CN107460121 A CN 107460121A CN 201710603421 A CN201710603421 A CN 201710603421A CN 107460121 A CN107460121 A CN 107460121A
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- mixing chamber
- pcr reaction
- reaction systems
- pcr
- fixed component
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
- B01L2200/147—Employing temperature sensors
Abstract
The present invention relates to a kind of preparation of PCR reaction systems and sampling device, it includes a fixed component offer portion and multiple variable composition provider, mixing chamber and sample feeding pipes, the outlet in fixed component offer portion connects with each mixing chamber, the entrance for the exporting corresponding mixing chamber connection of each variable composition provider, the outlet of each mixing chamber is connected with a sample feeding pipe, and the exit of mixing chamber is provided with outlet valve.Prepared by using the PCR reaction systems and sampling device, user can be not subjected to prepare the hard work of PCR system, especially when up to tens, sample is even hundreds of.Setting system is only needed to prepare parameter, pass through control system, indicate the respective components of addition set amount, it can be achieved to be automatically finished PCR system preparation, obtained PCR reaction systems are prepared to be pumped directly in tubular type temperature regulating device by sample feeding pipe, PCR reactions can be completed, need not artificially be participated between this process.
Description
Technical field
The present invention relates to molecular biology equipment, more specifically it relates to a kind of PCR reaction systems prepare and sampling device and
Full-automatic PCR instrument.
Background technology
PCR (PCR) is the underlying technology of molecular biology, and its principle is, using DNA at 95 DEG C or so
High temperature double-stranded DNA is unwind, after cooling, annealed between the primer of high concentration and the DNA profiling to unwind, then by reaction system
Temperature adjustment to polymerase activity temperature, make polymerase complementary along the direction composition along phosphoric acid to pentose (5 ' -3 ')
Chain.Actual PCR instrument based on polymerase manufacture is exactly a temperature control device, can be in denaturation temperature, renaturation temperature, elongating temperature it
Between be controlled well.
Since perkin-elmercetus companies First PCR instrument comes out, tens different producers are there is now in state
Inside and outside production and selling PCR instrument.Between short several years, PCR instrument passes through the development of several generations, constantly employs new technology, and enter
One step develops towards the direction of convenient, practical, high-intelligentization and automation.
Although round pcr obtained very big development, but all PCR instruments are all to control PCR by the following method
Reaction：PCR pipe equipped with PCR reaction systems is placed in the temperature-control hole of fixed temperature control base, and is close to the interior of temperature-control hole
Wall, the temperature of PCR pipe and its internal reaction system filled is controlled by controlling the temperature of temperature control hole wall.Although this kind of device
Generalized, however it there is some it is unconquerable the shortcomings that.
It is one of main shortcoming that time-consuming.Normal PCR reactions generally comprise 30 circulations, wherein denaturation 30s, annealing
30s, extension 30s/kb.Plus denaturation 5min, last eventually to extend 10min, expanding 1kb fragment only needs one and a half hours left side
The right time.However, when being reacted in PCR instrument, it has been found that the fragment for expanding 1kb was generally required at least over 2 hours
Time.The reason is that the position of sample is fixed in PCR instrument, therefore it is necessary to the temperature for manipulating temperature control base change to
React the temperature required for the stage proceeded to.30 circulations, every time circulation have 3 alternating temperatures, that is to say, that need to carry out 90 times
The alternating temperature of left and right.This needs to consume the substantial amounts of time close to the heating and cooling process of 100 times.
In addition to expending the time in heating and cooling process, existing PCR also have one it is huge the shortcomings that be, be not suitable for complete
Automation mechanized operation.Because circular response is carried out in fixed position, it is necessary to prepare reaction system in PCR pipe, then will be equipped with
The PCR pipe of reaction system is transferred in the reacting hole of PCR instrument.Due to being frequently necessary to prepare multiple reaction systems (for example, template is not
With, primer difference etc.), no matter system is prepared and the transfer of PCR pipe, is required for spending a large amount of manpowers, can not be accomplished full-automatic.Mesh
Before untill, also without any instrument can accomplish from PCR system be formulated into PCR reaction complete full-automation.
The content of the invention
Applicants have invented a kind of tubular type temperature regulating device, reaction system is entered from reaction tube inlet, by pipe shaft, reaction
The temperature of system and identical with the pipe shaft temperature of present position, and is reacted at such a temperature by heat transfer.
Based on the device, the invention provides a kind of preparation of PCR reaction systems and sampling device, it includes a fixed group
Point offer portion and multiple variable composition provider, mixing chamber and sample feeding pipes, the outlet in the fixed component offer portion with it is each
The mixing chamber connects, the entrance for the exporting corresponding mixing chamber connection of each variable composition provider, each
The outlet of the mixing chamber is connected with a sample feeding pipe, and the exit of the mixing chamber is provided with outlet valve.
Prepared by using the PCR reaction systems and sampling device, user can be not subjected to prepare the heavy of PCR system
Work, especially when up to tens, sample is even hundreds of.Only need setting system to prepare parameter, by control system, refer to
Show that the dosing pump set in fixed component offer portion and variable composition provider adds the respective components of set amount into mixing chamber,
It can be achieved to be automatically finished PCR system preparation, the PCR reaction systems for preparing to obtain are pumped directly tubular type control by sample feeding pipe
In warm device, you can complete PCR reactions, need not artificially be participated between this process.In general, in being reacted with a collection of PCR, enzyme,
The species and dosage of dNTP, buffer solution etc. are all fixed, and primer and template are likely to be fixed, it is also possible to are variable
, all fixed component of species and dosage is referred to as fixed component by we, and the component that species or dosage can be changed is call variable
Component.If for example, to detect the presence or absence of some gene in different samples, then enzyme, dNTP, buffer solution and primer pair
All it is fixed, these referred to as fixed components, and template is variable, referred to as variable composition.
In one embodiment, the fixed component offer portion is the device equipped with the fixed component of mixing.
Specifically, the every other component in addition to variable composition is provided in the fixed component offer portion in PCR reaction systems
Mixture.
In one embodiment, the fixed component offer portion includes multiple fixed component provider and a premix
Room, the entrance exported with the premixer of each fixed component provider connect, the outlet of the premixer and institute
State the entrance connection of mixing chamber.Fixed component provider is respectively provided with each fixed component, and passes through measuring pump according to the value of setting
It is input in premixer, so as to obtain the mixture available for multiple PCR reacting doses, measuring pump in premixer is by a PCR
In the mixture input mixing chamber of the amount of reaction, the variable composition of the amount of setting is added to by the measuring pump of variable composition provider
In mixing chamber, so as to be configured to PCR reaction systems.
Preferably, the multiple fixed component provider supplies PCR reaction buffers, nucleosides to the premixer respectively
Acid, polymerase, the one kind also supplied to the premixer in template and primer pair.
Preferably, the multiple variable composition provider provides different primer pairs to the mixing chamber respectively, or different
Template, or the same template of various concentrations.
Present invention also offers a kind of full-automatic PCR instrument, it include above-mentioned PCR reaction systems prepare and sampling device with
And tubular type temperature regulating device, the PCR reaction systems are prepared and sampling device passes through the sample feeding pipe with the tubular type temperature regulating device
Connection.
Brief description of the drawings
Fig. 1 is the preparation of PCR reaction systems and the schematic diagram of sampling device of embodiment 1；
Fig. 2 is the preparation of PCR reaction systems and the schematic diagram of sampling device of embodiment 2；
Fig. 3 is the schematic diagram of an example of temperature regulating device；
Fig. 4 is the viewgraph of cross-section of one example of temperature regulating device；
Fig. 5 is the viewgraph of cross-section of an example of temperature regulating device；
Fig. 6 is the schematic diagram of an example of temperature regulating device；
In accompanying drawing, the list of parts representated by each label is as follows：
1st, fixed component offer portion, 101, fixed component provider, 102, premixer, 2, variable composition provider, 3, mixed
Close room, 4, sample feeding pipe, 5, temperature control post, 6, reaction tube pipe shaft, 7, entrance, 8, outlet, 9, reaction product discharge pipe, 501, denaturation
Area, 502, annealed zone, 503, extension area, 601, denaturation section, 602, annealing section, 603, extension of section, 604, insulation short tube,
701st, outlet valve, 801, valve.
Embodiment
The principle and feature of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Embodiment 1
As shown in figure 1, the PCR reaction systems of the embodiment are prepared and sampling device includes a fixed component offer portion 1
And multiple variable composition provider 2, mixing chamber 3 and sample feeding pipe 4, the outlet in the fixed component offer portion 1 with it is each described
Mixing chamber 3 connects, the entrance for exporting corresponding mixing chamber 3 connection of each variable composition provider 2, Mei Gesuo
The outlet for stating mixing chamber 3 is connected with a sample feeding pipe 4, and the exit of the mixing chamber 3 is provided with outlet valve.Described fixed group
Point offer portion 1 is the device equipped with the fixed component of mixing, is equipped with the fixed component offer portion 1 in PCR reaction systems except can
Become the mixture of every other component outside component.
Prepared by using the PCR reaction systems and sampling device, user can be not subjected to prepare the heavy of PCR system
Work, especially when up to tens, sample is even hundreds of.Only need setting system to prepare parameter, by control system, refer to
Show that the dosing pump set in fixed component offer portion 1 and variable composition provider 2 adds the respective sets of set amount into mixing chamber 3
Point, you can realization is automatically finished PCR system preparation, and after the completion of preparation, outlet valve is opened, and PCR reaction systems pass through sample feeding pipe 4
It is pumped directly in tubular type temperature regulating device, you can complete PCR reactions, need not artificially be participated between this process.In general, it is same
To criticize in PCR reactions, the species and dosage of enzyme, dNTP, buffer solution etc. are all fixed, and primer and template are likely to be fixed,
It could also be possible that variable, all fixed component of species and dosage is referred to as fixed component by we, and species or dosage can be changed
Component be referred to as variable composition.If for example, to detect the presence or absence of some gene in different samples, then enzyme, dNTP,
Buffer solution and primer pair are all fixed, these referred to as fixed components, and template is variable, referred to as variable composition.At this
, it is necessary to first prepare fixed component mixture in embodiment, add mixture in fixed component offer portion 1.
Embodiment 2
As shown in Fig. 2 the PCR reaction systems of the embodiment are prepared and sampling device includes a fixed component offer portion 1
And multiple variable composition provider 2, mixing chamber 3 and sample feeding pipe 4, the outlet in the fixed component offer portion 1 with it is each described
Mixing chamber 3 connects, the entrance for exporting corresponding mixing chamber 3 connection of each variable composition provider 2, Mei Gesuo
The outlet for stating mixing chamber 3 is connected with a sample feeding pipe 4, and the exit of the mixing chamber 3 is provided with outlet valve.
The fixed component offer portion 1 includes multiple fixed component provider 101 and a premixer 102, each described
Entrance of the outlet of fixed component provider 101 with the premixer 102 connects, the outlet of the premixer 102 with it is described
The entrance connection of mixing chamber 3.The multiple fixed component provider 101 is respectively to the premixer 102 supply PCR reaction bufferings
Liquid, nucleotides, polymerase, the one kind also supplied to the premixer 102 in template and primer pair.The multiple variable composition carries
For device 2 different primer pairs, or different templates, or the same template of various concentrations are provided to the mixing chamber 3 respectively.It is fixed
Component provider 101 is respectively provided with each fixed component, and is input to according to the value of setting by measuring pump in premixer 102, from
And the mixture available for multiple PCR reacting doses is obtained, the measuring pump in premixer 102 is by the mixing of the PCR amounts reacted
In thing input mixing chamber, the variable composition of the amount of setting is added in mixing chamber by the measuring pump of variable composition provider 2, so as to
It is configured to PCR reaction systems.
Embodiment 3
The full-automatic PCR instrument of the present invention includes above-mentioned PCR reaction systems preparation and sampling device and tubular type temperature control dress
Put, the PCR reaction systems are prepared and sampling device is connected with the tubular type temperature regulating device by the sample feeding pipe 4.
As shown in figure 3, the temperature regulating device includes temperature control post 5 and reaction tube, the reaction tube includes entrance 7, outlet 8 and pipe
Body 6, the pipe shaft 6 of the reaction tube are spirally coiled in 5 on the temperature control post, and the entrance 7 connects with sample feeding pipe 4, the outlet 8
Connected with reaction product discharge pipe 9.
In running, reaction system is entered by sample feeding pipe 4 from entrance 7, and by pipe shaft 6, the temperature of reaction system is led to
Cross heat transfer and identical with the temperature of pipe shaft 6 of present position, and reacted at such a temperature, completed after reaction from reaction product
Discharge pipe 9 comes out.Such tubular type temperature regulating device can be used for the reaction environment for building isothermal reaction and temperature cycles reacting condition.
In another embodiment, as shown in figure 4, the periphery of the cross section of temperature control post 5 of temperature regulating device is divided into three
Heated at constant temperature area, degeneration area 501, annealed zone 502 and extension area 503 being followed successively by, the temperature of the degeneration area 501 is 90-98 DEG C,
The temperature of the annealed zone 502 is 45-70 DEG C, and the temperature of the extension area 503 is 70-75 DEG C, the entrance of the reaction tube 7
In one end by the annealed zone 503 of the degeneration area 501, the outlet 8 of the reaction tube is located at the extension area 503 and leans on institute
State one end of degeneration area 501.The device can be used in PCR instrument, the temperature regulating device as PCR reactions.
The division in three heated at constant temperature areas to be formed the temperature needed for the three phases of a PCR cycle in reaction tube
Degree, and in the temperature regulating device, these three warm areas are all constant temperature, it is not necessary to denaturation temperature-annealing temperature-elongating temperature it
Between carry out cyclic switching.Reaction system flows successively through annealed zone, extension area since degeneration area, and the temperature for completing a PCR is followed
Ring, so as to often once complete the PCR reactions of a circulation around the operation of temperature control post.
In another embodiment, heated at constant temperature area may include multiple heated at constant temperature units, part heated at constant temperature unit
Degeneration area is formed, part forms annealed zone, and part forms extension area.Can be by changing the quantity of the heated at constant temperature unit in each area
To adjust the length in each area, so as to control the reaction time.
In another embodiment, temperature control post and pipe shaft can be divided into three sections, and one section is used for the initial denaturation stage, one section with
In the circular response stage, also one section is used for the last extension stage eventually.
In another embodiment, as shown in figure 5, the pipe shaft 6 of temperature regulating device reaction tube is according to residing heated at constant temperature area
Segmentation, often enclose spiral and correspondingly form three constant temperature sections, be followed successively by denaturation section 601, annealing section 602 and extension of section
603, and connected between adjacent section by short tube made of heat insulator 604.By being adopted between adjacent section
Take the short tube 604 of heat insulation to connect, preferably avoid the heat transfer between the different constant temperature sections of pipe shaft 6.In the present embodiment
So-called heat insulator refers to the relatively low material of pyroconductivity, rather than definitely athermanous material.
In a preferred scheme, the surface of temperature control post 5 has the bung flange to match with the reaction tube pipe shaft 6
Groove, the reaction tube pipe shaft 6 are embedded in the bung flange groove.The setting makes it possible to more preferably precisely control reaction tube pipe
The temperature of body 6.It is highly preferred that separated between heated at constant temperature area corresponding to the bung flange groove by heat insulator, preferably every
From the heat transfer between adjacent heated at constant temperature area.So-called heat insulator refers to the relatively low material of pyroconductivity in the present embodiment
Material, rather than definitely athermanous material.
In another embodiment, as shown in fig. 6, the pipe shaft has multiple outlets 8, it is located at respectively away from the entrance
At 7 different bung flange numbers, and each outlet 8 is both provided with outlet valve 801, and the outlet 8 farthest from entrance 7 can be not provided with exporting
Valve 801.The outlet valve 801 causes residing exit to have two states, and a state is reaction system to next spiral shell
Circle movement, another state is the pipe shaft 6 that reaction system leaves reaction tube, is come out from outlet.For example, reaction tube pipe shaft 6 can be
30-60 circles are coiled on temperature control post 1, and outlet 8 is set at the number of turns different away from entrance, setting the state of outlet valve 801 makes instead
System is answered to be come out by the specific number of turns and from corresponding outlet 8 by the reaction product discharge pipe 9 with the outlet, so as to
Carry out specific PCR reaction cycle numbers.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (7)
1. a kind of PCR reaction systems are prepared and sampling device, it is characterised in that including a fixed component offer portion (1) and
Multiple variable composition provider (2), mixing chamber (3) and sample feeding pipe (4), the outlet of the fixed component offer portion (1) with it is each
The mixing chamber (3) connects, the entrance for exporting corresponding mixing chamber (3) of each variable composition provider (2)
Connection, the outlet of each mixing chamber (3) are connected with a sample feeding pipe (4), and the exit of the mixing chamber (3) is provided with
Outlet valve.
2. PCR reaction systems according to claim 1 are prepared and sampling device, it is characterised in that the fixed component carries
It is the device equipped with the fixed component of mixing for portion (1).
3. PCR reaction systems according to claim 2 are prepared and sampling device, it is characterised in that the fixed component carries
Mixture for being equipped with the every other component in addition to variable composition in PCR reaction systems in portion (1).
4. PCR reaction systems according to claim 1 are prepared and sampling device, it is characterised in that the fixed component carries
Include multiple fixed component provider (101) and a premixer (102) for portion (1), each fixed component provider
(101) entrance of the outlet with the premixer (102) connects, outlet and the mixing chamber (3) of the premixer (102)
Entrance connection.
5. PCR reaction systems according to claim 4 are prepared and sampling device, it is characterised in that the multiple fixed group
Provider (101) is divided to supply PCR reaction buffers, nucleotides, polymerase to the premixer (102) respectively, also to described pre-
One kind in mixed room (102) supply template and primer pair.
6. the PCR reaction systems according to any one of claim 1-5 are prepared and sampling device, it is characterised in that described
Multiple variable composition provider (2) provide different primer pairs, or different templates to the mixing chamber (3) respectively, or different
The same template of concentration.
7. a kind of full-automatic PCR instrument, it is characterised in that prepared including the PCR reaction systems any one of claim 1-6
And sampling device and tubular type temperature regulating device, the PCR reaction systems are prepared and sampling device leads to the tubular type temperature regulating device
Cross the sample feeding pipe (4) connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710603421.7A CN107460121A (en) | 2017-07-22 | 2017-07-22 | A kind of PCR reaction systems are prepared and sampling device and full-automatic PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710603421.7A CN107460121A (en) | 2017-07-22 | 2017-07-22 | A kind of PCR reaction systems are prepared and sampling device and full-automatic PCR instrument |
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| CN107460121A true CN107460121A (en) | 2017-12-12 |
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| CN201710603421.7A Pending CN107460121A (en) | 2017-07-22 | 2017-07-22 | A kind of PCR reaction systems are prepared and sampling device and full-automatic PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021000750A1 (en) * | 2019-07-01 | 2021-01-07 | 申翌生物科技(杭州)有限公司 | Novel method for performing pcr reaction using comprehensive pcr reaction system |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922318A (en) * | 2003-12-24 | 2007-02-28 | 3M创新有限公司 | Methods for nucleic acid isolation and kits using solid phase material |
| CN2896284Y (en) * | 2006-04-06 | 2007-05-02 | 北京工业大学 | Simple pneumatic sample-feeding device of continuous-flow type PCR micro flow control chip |
| JP2008278810A (en) * | 2007-05-11 | 2008-11-20 | Sony Corp | Real-time pcr apparatus |
| CN201901669U (en) * | 2010-07-23 | 2011-07-20 | 浙江大学 | Integrated flow path chip device for digital nucleic acid amplification |
| CN102304464A (en) * | 2011-10-08 | 2012-01-04 | 武汉馨世生物科技有限公司 | Multiplex polymerase chain reaction amplification instrument |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微系统与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
| CN103602659A (en) * | 2013-10-23 | 2014-02-26 | 杨锦宇 | High-throughput nucleic acid continual amplification apparatus and method |
| CN103667046A (en) * | 2013-12-19 | 2014-03-26 | 江苏金太生命科技有限公司 | PCR (polymerase chain reaction) instrument with partitions |
| CN105277725A (en) * | 2014-07-01 | 2016-01-27 | 清华大学 | Integrated micro-fluidic system for nucleic acid analysis and detection |
| CN105349401A (en) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
-
2017
- 2017-07-22 CN CN201710603421.7A patent/CN107460121A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1922318A (en) * | 2003-12-24 | 2007-02-28 | 3M创新有限公司 | Methods for nucleic acid isolation and kits using solid phase material |
| CN2896284Y (en) * | 2006-04-06 | 2007-05-02 | 北京工业大学 | Simple pneumatic sample-feeding device of continuous-flow type PCR micro flow control chip |
| JP2008278810A (en) * | 2007-05-11 | 2008-11-20 | Sony Corp | Real-time pcr apparatus |
| CN201901669U (en) * | 2010-07-23 | 2011-07-20 | 浙江大学 | Integrated flow path chip device for digital nucleic acid amplification |
| CN102304464A (en) * | 2011-10-08 | 2012-01-04 | 武汉馨世生物科技有限公司 | Multiplex polymerase chain reaction amplification instrument |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微系统与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
| CN103602659A (en) * | 2013-10-23 | 2014-02-26 | 杨锦宇 | High-throughput nucleic acid continual amplification apparatus and method |
| CN103667046A (en) * | 2013-12-19 | 2014-03-26 | 江苏金太生命科技有限公司 | PCR (polymerase chain reaction) instrument with partitions |
| CN105277725A (en) * | 2014-07-01 | 2016-01-27 | 清华大学 | Integrated micro-fluidic system for nucleic acid analysis and detection |
| CN105349401A (en) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 侯新东主编: "《生物化学与分子生物学实验教程》", 31 December 2016, 中国地质大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021000750A1 (en) * | 2019-07-01 | 2021-01-07 | 申翌生物科技(杭州)有限公司 | Novel method for performing pcr reaction using comprehensive pcr reaction system |
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