CN107446852A - One lactobacillus plantarum and its application in terms of fermented feed - Google Patents
One lactobacillus plantarum and its application in terms of fermented feed Download PDFInfo
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- CN107446852A CN107446852A CN201710749942.3A CN201710749942A CN107446852A CN 107446852 A CN107446852 A CN 107446852A CN 201710749942 A CN201710749942 A CN 201710749942A CN 107446852 A CN107446852 A CN 107446852A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Application the invention discloses a lactobacillus plantarum and its in terms of fermented feed, belong to fermented feed technical field.Lactobacillus plantarum (Lactobacillus plantarum) JUN DY 6 of the present invention, China typical culture collection center is preserved on March 23rd, 2017, deposit number is CCTCC M 2017138, and preservation address is that preservation address is China, Wuhan, Wuhan University.The bacterial strain acid spectrum of the present invention is wide, and production acid amount is high, and there is broad-spectrum antibacterial to act on, the content of ANFs in fermented bean dregs can effectively be reduced, the incidence of diarrhoea is reduced, that improves nutriment utilizes level, and the storage phase of feed can effectively be extended by preparing feed using the bacterial strain.
Description
Technical field
Application the present invention relates to a lactobacillus plantarum and its in terms of fermented feed, belong to fermented feed technology neck
Domain.
Background technology
With the fast development of aquaculture, from last century the fifties, breeding enterprise is increase animal productiong amount, dynamic
Antibiotic is added in thing diet, and the development to whole cultivation industry brings tremendous contribution.But over time, resist
Raw plain use is more and more frequent, and harm increasingly shows, and causes personages of various circles of society's highest attention.Feed safety is equal at present
In food security concept worldwide oneself turn into common recognition.Research finds that fermented feed can suppress pathogenic bacteria in alimentary canal
Growth, Animal diseases are played with certain prevention effect, meanwhile, it also has, and palatability is good, nutrient absorption utilization rate is high, can
The advantages that substitute antibiotics use.
Fermentative feedstuff of microbe (Fermented feed) refer to it is artificial it is controllable under the conditions of, with the agricultural and sideline production of vegetalitas
Product are primary raw material, raw by beneficial microorganism metabolism, the macromolecular substances such as degraded par-tial polysaccharide, protein and fat
The small-molecule substance for being easy to digest and assimilate into organic acid, soluble polypeptide etc., it is good, nutritious, effectively prebiotic to form palatability
The high biological feedstuff of bacterial content, its active ingredient mainly include beneficial microbe mycoprotein, the various biology enzymes easily absorbed
Class, bioactivity widow skin, amino acid, organic acid, active probiotic etc.
Microorganism currently used for fermented feed production mainly includes：Bacillus, saccharomycete, aspergillus class fungi and breast
Sour bacterium.Lactic acid bacteria is on the one hand to produce antibiotic in fermented feed and suppress and kill harmful bacteria to prevent it from growth and breeding,
Reducing or kill harmful substance makes endotoxin content reduction make former host resist different ability to strengthen the dominance in competition so as to strengthen,
On the other hand the nutritive value of feed can be improved, carbohydrate breakdown is improved into material flavor and palatability.However, existing lactic acid
It is less to have the bacterial strain of acid producing ability and antagonistic property in bacterium concurrently, is usually to mix thalline system, the breast being exogenously introduced plus feed fermentation
Sour bacterium easily breaks the mixed microorganism balance in fermented feed, the nutritive value of fermented feed is improved limitation, shelf-life
Do not grow.
The content of the invention
First purpose of the present invention is to provide a kind of Lactobacillus plantarum (Lactobacillus plantarum) JUN-
DY-6, is preserved in China typical culture collection center on March 23rd, 2017, and deposit number is CCTCC M
2017138, preservation address be preservation address be China, Wuhan, Wuhan University.
Second object of the present invention is to provide the cultural method of the Lactobacillus plantarum, and methods described is to be inoculated in MRS
In culture medium, 30~37 DEG C of 24~48h of culture.
Third object of the present invention is to provide a kind of bacteriostasis method, and methods described is to be seeded to the Lactobacillus plantarum
In liquid, semisolid or solid containing pathogenic bacteria, the miscellaneous bacteria is included in Escherichia coli, staphylococcus aureus or salmonella
At least one.
Fourth object of the present invention is to provide a kind of feed, and the feed contains the Lactobacillus plantarum and feed and carried
Body.
In one embodiment of the invention, the feed vector is included in dregs of beans, soya-bean cake, starch, molasses at least
It is a kind of.
The 5th purpose of the present invention is to provide the preparation method of the feed, and methods described is by the Lactobacillus plantarum
It is seeded in feed vector, 5~7d is left to ferment in 30~37 DEG C.
The 6th purpose of the present invention is to provide a kind of method for extending the fermented feed shelf-life, is by described plant breast
Bacillus JUN-DY-6 is added in feed vector, is fermented.
The 7th purpose of the present invention is to provide the application of the Lactobacillus plantarum.
In one embodiment of the invention, the application includes preparing food, medicine, health products.
Beneficial effect：1st, Lactobacillus plantarum JUN-DY-6 of the invention acid spectrum is wide, and production acid amount is high, and growth rapidly can be with dregs of beans
A variety of organic acids are produced for raw material.And the 28h total acid contents that fermented under the conditions of 37 DEG C can reach 14.82g/L；2nd, using the present invention's
Strain fermentation dregs of beans, high molecular weight protein in fermented bean dregs can be made to degrade substantially totally, can effectively reduce fermented bean dregs moderate resistance nutrition
The content of the factor, the incidence of diarrhoea is reduced, that improves nutriment utilizes level；3rd, the feeding prepared using the bacterial strain of the present invention
Material is beneficial to storage, fermented bean dregs wet feed can be made to be preserved 40~60 days under normal temperature condition, than un-added feed extended shelf-life
2~3 times, and there is no mildew phenomena.
Brief description of the drawings
Fig. 1：Lactobacillus plantarum JUN-DY-6 growth curves；
Fig. 2：Lactobacillus plantarum JUN-DY-6 growth course pH change curves；
Fig. 3：Different process fermented bean dregs PAGE gel electrophoretic analysis；Wherein, Marker represents reference band, SM
Unleavened dregs of beans, 0% represents to the addition of the fermented bean dregs that Lactobacillus plantarum is not added with alkali protease, and 0.5% represents to add
Add Lactobacillus plantarum while with the addition of the fermented bean dregs of the alkali protease of mass fraction 0.5%.
Biomaterial preservation
One lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6, in preservation on March 23 in 2017
In China typical culture collection center, deposit number is CCTCC M 2017138, and preservation address is that preservation address is China,
Wuhan, Wuhan University.
Embodiment
The measure of organic acid content：Use Hitachi's high performance liquid chromatograph, ultraviolet absorption detector, analytical column organic acid
Post (Aninex Hpx-87H ion exchange column), 50 DEG C of column temperature, mobile phase 5mM sulfuric acid solutions, flow velocity 0.6mL/
Min, sample size 10 μ L, detection time 27min, calculate the content of various organic acids in sample.
Embodiment 1
1：To reach preferable separating effect, fermented bean dregs are accessed into MRS fluid nutrient mediums by inoculum concentration 10g/100mL
In, 30 DEG C, 200rpm culture 48h, continuous Multiplying culture 2~3 times.
MRS culture medium prescriptions are as follows：Peptone 10.0g, beef leach thing 10.0g, yeast extract 5.0g, glucose
5.0 g, sodium acetate 5.0g, lemon acid diamine 2.0g, Tween-80 1.0g, dipotassium hydrogen phosphate 2.0g, epsom salt 0.2g, seven
Water manganese sulfate 0.05g, calcium carbonate 20.0g, agar 20.0g, distilled water are settled to 1.0L, pH 6.8.
2：Strain isolates and purifies：With sterile saline dilute sample step by step, the separation training prepared is coated on
Support on base (contain 2% calcium carbonate) flat board, cover one layer of same culture medium again thereon, interlayer Anaerobic culturel (30 DEG C, 48h),
Then the big single bacterium colony of molten calcium circle is selected, repeatedly line is isolated and purified to obtain pure culture and is named as on isolation medium
JUN-DY-6。
3：The 16SrDNA identifications of bacterial strain
Bacterial strain JUN-DY-6 genomic DNAs are extracted with bacterial genomes DNA Rapid extractions kit (raw work biology), as
PCR expands template, using 165rDNA primer pairs (primer 2 7F and primer 1495), enters performing PCR amplification.
PCR courses of reaction are as follows:95 DEG C of denaturation 5min；95 DEG C/30s, 55 DEG C/30s, 72 DEG C/2min, 35 circulations, 72
DEG C keep 5min.PCR primer is obtained into band brighter about at 1500bp through electrophoretic analysis, with Q-quick Gel
Extraction Kit (Promeg companies) carry out gel extraction purifying, the sequencing of PCR purified product Song Sheng works biotech firm.It will survey
The sequence of bacterial strain and Genbank databases after sequence carries out sequence analysis, and it is Lactobacillus plantarum to draw bacterial strain JUN-DY-6
(Lactobacillus plantarum)。
Embodiment 2
1st, the preparation of indicator bacteria bacteria suspension：The pathogenic bacteria glycerol tube such as Escherichia coli, salmonella and staphylococcus aureus
It is inoculated in culture medium, cultivates 16-18 hours in 37 DEG C of shaking table 200r/min, bacterium is dense up to 1.2 × 1010CFU/mL。
2nd, the preparation of Bacillus acidi lactici bacteria suspension：Purebred Lactobacillus plantarum is inoculated in 100mL MRS liquid in aseptic condition
In body culture medium, 37 DEG C of 24~48h of quiescent culture, bacterium is dense up to 1.5 × 1010CFU/mL。
3rd, the preparation of double-layer plate takes diameter about 90mm flat board, pours into the nutrient agar 18 of heating and melting respectively
~20mL, it is set uniformly to spread out cloth in flat board, place makes solidification on level table, as bottom.Separately take semisolid nutrient agar fat
After the appropriate heating and melting of culture medium (agar content 1%), let cool to 48-50 DEG C, every 50~100mL culture mediums add indicator bacteria
0.1~0.2mL of bacteria suspension, 5mL is separately added into every 1 flat board, it is uniformly spread out cloth on bottom, as bacterium layer.Place
It is standby with equidistant uniformly placement Oxford cup 4 in every 1 flat board after being cooled down on level table.
Wherein nutrient agar formula is as follows：Tryptone 10g, yeast extract 5g, NaCl 10g deionizations
Water, which is settled to 1L. solid mediums, need to add 15~20g agar, and semisolid culturemedium need to add 10g agar.
4th, bacteriostasis property is evaluated：Take step 2 fermented liquid supernatant, drop fills 200 μ respectively in the Oxford cup in each double-layer plate
After L, 37 DEG C of culture 18h, each antibacterial circle diameter (or area) is measured to make an appraisal.Typical plate is chosen to take a picture.
Biocidal property：Inhibition zone<10mm is that fungistatic effect is faint, 10mm<Inhibition zone<15mm for moderate it is antibacterial, inhibition zone>
15mm to be highly antibacterial.
Remarks：1) the bigger explanation fungistatic effect of inhibition zone is better；2) inhibition zone refers to the whole diameter of circular non-opaque circle.
Table 1 is bacteriostasis (unit mm) of the Lactobacillus plantarum JUN-DY-6 to different microorganisms
The Lactobacillus plantarum JUN-DY-6 of table 2 counts to the antibacterial result of pathogenic bacteria
As shown in table 1-2, bacterial strain JUN-DY-6 is significantly antibacterial to Escherichia coli, staphylococcus aureus and salmonella
Effect, antibacterial circle diameter up to 12~22mm, 12.77~15.9mm, 12.4~16.3mm, average value 18.0mm, 14.8mm,
13.9mm, standard deviation are less than 0.1.
Embodiment 3
Reference《The outstanding Bacteria Identification handbook of uncle》With《Lactic acid bacteria taxonomic identification and experimental method》, and to bacterial strain JUN-DY-6
Carry out 16sRNA sequencings.Wherein bacterial strain bio-chemical characteristics result is as shown in table 4, and carbon source through fermentation result of the test is as shown in table 3, knot
Physiology and biochemistry and carbon source through fermentation experimental result are closed, and 16S information is compared.According to cell microscopic morphology, Physiology and biochemistry number
According to 16S rRNA gene sequence datas, bacterial strain is accredited as Lactobacillus plantarum (Lactobacillus plantarum).
Table 3:Lactobacillus plantarum JUN-DY-6 sugar fermentating test result.
Note："+" represents strong fermentation, and " d " represents slightly to ferment, and "-" represents azymic
Table 4:Lactobacillus plantarum JUN-DY-6 Physicochemical test result.
Note:"-" represents feminine gender, and "+" represents the positive.
Embodiment 4
Fermented bean dregs, specific steps are produced using Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6
It is as follows：
By air-dried dregs of beans 100g, water 50g, the Lactobacillus plantarum bacterium solution (OD 7~8) of molasses 3g and 3~10mL logarithmic phase
It is placed in after well mixed in the valve bag through aseptic process, sealing is placed in 37 DEG C of incubators the 48h that ferments.Take once within every four hours
Sample.Per batch experiment do three it is parallel.4g fermented bean dregs are taken in 50ml centrifuge tubes per sub-sampling, are added 40ml deionized waters and are stirred
Mix after mixing stands 1h and take supernatant, be placed in after 0.22 μm of membrane filtration in liquid phase bottle and be used for efficient liquid phase chromatographic analysis, as long as
Analyze the species and content of organic acid in sample.3g dregs of beans is weighed after fermentation ends in 100ml beakers, adds 30mL,
0.2%NaOH stirs 15min at room temperature, centrifuges 7min in 6000r/min, abandons lipid layer and precipitation, carefully leave and take supernatant 2mL,
5 times of dilution keeps sample.Take the 30 diluted sample supernatants of μ L, add 10 μ 4 × buffer of L, boiling water bath 10min, 13000rpm from
Heart 5min supernatant loadings, carry out the degraded situation of macromolecular substances in PAGE gel electrophoretic analysis fermented bean dregs.As a result
As shown in figure 3, SM is the protein content in thick dregs of beans, 0% is the protein content in the fermented bean dregs of addition Lactobacillus plantarum.By
Figure understands that the production that Lactobacillus plantarum is used for fermented bean dregs can obvious degradation macromolecular substances therein.
Embodiment 5
Using Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6 and add alkali protease production hair
Ferment dregs of beans：
By air-dried dregs of beans 100g, water 50g, molasses 3g, the plant breast bar of alkali protease 0.5g and 3~10mL logarithmic phase
It is placed in after bacterium bacterium solution (OD 7~8) is well mixed in the valve bag through aseptic process, sealing, which is placed in 37 DEG C of incubators, ferments
48h.Take a sample within every four hours.Per batch experiment do three it is parallel.Sampling and processing mode are same as above.
Table 5 is the kinds of organic acids and highest content (g/L) of different fermentations technique productions fermented bean dregs
Note：G1 represents only addition Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6 groups；G2 represents to add
Add Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6 and alkali protease group.Production when being 28h or so
Acid number.
From in table, fermented bean dregs are produced with Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6,
In 48h the bacterium can amount reproduction produce acid rapidly, and the wide production acid amount of acid spectrum is high.Its highest when being used cooperatively with alkali protease
Production production acid amount can reach 14.82g/L.Found by PAGE gel electrophoresis (Fig. 3), addition Lactobacillus plantarum JUN-DY-6 can
By the protein degradation in dregs of beans about 50%, and production fermented bean dregs not only produce sour amount when the bacterium is used cooperatively with a small amount of protease
Height, and high molecular weight protein is degraded totally substantially.
Embodiment 5
Using Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6 and add alkali protease production hair
The shelf stability experiment of ferment dregs of beans：
Dregs of beans 100g, water 50g, molasses 3g that will be air-dried, (enzyme-activity unit of alkali protease be alkali protease 0.5g
15000~20000U/g) and 3~10mL logarithmic phases Lactobacillus plantarum bacterium solution (OD 7~8) it is well mixed after be placed in through sterile
In the valve bag of processing, sealing is placed in 37 DEG C of incubators the 48h that ferments.Using do not add the fermented bean dregs of Lactobacillus plantarum as pair
According to.
Fermented bean dregs wet feed is placed in room temperature after the completion of fermentation and stored, routine observation its whether go mouldy and (occur
Mildew).As a result display by Lactobacillus plantarum JUN-DY-6 and add the fermented bean dregs wet feed of the production of alkali protease can
Preserve 40~60 days and do not go mouldy at room temperature.And control group (other dispensing all sames in addition to Lactobacillus plantarum is not added)
It can only be preserved under wet feed normal temperature about 20 days.The production of fermented bean dregs, the storage of its wet feed are carried out with commercialized Lactobacillus plantarum
Phase was at 30 days or so.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, it can all do various change and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (10)
1. a kind of Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6, is preserved on March 23rd, 2017
China typical culture collection center, deposit number are CCTCC M 2017138, and preservation address is that preservation address is China, military
The Chinese, Wuhan University.
2. a kind of method of suppression pathogenic bacteria for non-disease Clinics and Practices purposes, it is characterised in that by claim 1 institute
The Lactobacillus plantarum JUN-DY-6 stated is seeded in the liquid containing pathogenic bacteria, semisolid or solid.
3. according to the method for claim 2, it is characterised in that the pathogenic bacteria include Escherichia coli, Staphylococcus aureus
At least one of bacterium or salmonella.
4. a kind of feed, it is characterised in that the feed contains Lactobacillus plantarum JUN-DY-6 and the feed described in claim 1
Carrier.
5. feed according to claim 4, it is characterised in that the feed vector includes dregs of beans, soya-bean cake, starch, molasses
At least one of.
A kind of 6. method for preparing feed described in claim 5, it is characterised in that the Lactobacillus plantarum is seeded to feed and carried
In body, 5~7d is left to ferment in 30~37 DEG C.
7. according to the method for claim 6, it is characterised in that also add protease before fermentation；The addition of protease is
75~100U/g feed vectors.
A kind of 8. method for extending the fermented feed shelf-life, it is characterised in that by the Lactobacillus plantarum JUN- described in claim 1
DY-6 is added in feed vector, is fermented.
9. applications of the Lactobacillus plantarum JUN-DY-6 in food, medicine, chemical field described in claim 1.
10. contain the food of Lactobacillus plantarum JUN-DY-6 or its active component, medicine, health products described in claim 1.
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| CN109287847A (en) * | 2018-09-27 | 2019-02-01 | 江南大学 | A kind of preparation method of the fermented bean dregs rich in phenyllactic acid |
| CN109198162A (en) * | 2018-10-29 | 2019-01-15 | 江南大学 | A kind of method that bacterium enzyme cooperative fermentation prepares dregs of beans |
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| CN111557377B (en) * | 2020-05-12 | 2022-09-27 | 江南大学 | Method for preparing fruit and vegetable waste fermented feed |
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| CN111676153B (en) * | 2020-05-30 | 2022-05-31 | 青岛玛斯特生物技术有限公司 | Lactobacillus plantarum and application thereof in aquatic product fermented feed |
| CN113261613A (en) * | 2021-06-23 | 2021-08-17 | 贵州理工学院 | Method for preserving wet white spirit vinasse and preventing mildew and feed |
| CN113355265A (en) * | 2021-06-30 | 2021-09-07 | 广东温氏大华农生物科技有限公司 | Lactobacillus plantarum and application thereof |
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