CN107442191A - A kind of centrifugal type microfludic chip for Water-In-Oil drop formation - Google Patents
A kind of centrifugal type microfludic chip for Water-In-Oil drop formation Download PDFInfo
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- CN107442191A CN107442191A CN201710855088.9A CN201710855088A CN107442191A CN 107442191 A CN107442191 A CN 107442191A CN 201710855088 A CN201710855088 A CN 201710855088A CN 107442191 A CN107442191 A CN 107442191A
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- drop
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
- B01L2300/166—Suprahydrophobic; Ultraphobic; Lotus-effect
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
Abstract
The invention discloses a kind of centrifugal type microfludic chip for Water-In-Oil drop formation.Its chip structure includes channel layer and confining bed.Its micro-fluidic chip channel design includes aqueous phase storage area, interface channel, drop formation area and drop collecting region.Its operating procedure includes：(1) aqueous sample of certain volume is added in aqueous phase area, the oil phase of certain volume is added in drop collecting region.(2) centrifuge, generate drop, drop is collected automatically in drop storage area.The invention can be applied to the research application field such as biology, chemistry, medical diagnosis.
Description
Technical field
The present invention relates to a kind of centrifugal type microfludic chip for Water-In-Oil drop formation, applied to micro-fluidic theoretical meter
Point counting is analysed, the field such as biology, chemical detection analysis, medical diagnosis.
Background technology
Microlayer model has the advantages that small volume, flux are high, size is homogeneous, internal stability, controllable is widely used in giving birth to
The fields such as thing, chemical detection analysis, medical diagnosis, theoretical calculation, materials synthesis.Traditional drop formation is to be based on fluid focus
Cake core, this drop formation need precise injection pump to carry out strict control to channel fluid flow rate, and operation is very difficult.
M.Nakajima seminars in 1997 propose a kind of staged chip structure and are used for drop formation, and its operation principle is fluid stream
Generation drop is ruptured through static instability occurs during three-dimensional step, although this drop formation mode reduces rate of flow of fluid
Influence for drop, but drop formation highly relies on the ledge structure of complexity, chip manufacture difficulty is big.Du Wen is refined within 2015
Seminar proposes the mode based on capillary end microseismic activity to realize drop formation, and this drop formation depends on aqueous phase stream
The frequency of flow velocity and the capillary vibrations of body, is more difficult to manipulate relative to micro-fluidic chip.Huangyan friendship seminar proposes within 2016
Drive the mode of generation drop based on capillary array combination inertia force, and capillary array big, the limit that makes batch wise differences
Its application is made.Based on existing drop formation mode inevitably it is complicated or manipulation it is difficult the shortcomings that, the present invention devises
It is a kind of simple in construction, operate easy centrifugal type microfludic chip and be used for drop formation.
The content of the invention
Drop formation method fluid of the invention for fluid focus such as existing cross or T fonts is unstable, needs
The shortcomings that precise injection pump is strictly controlled inner fluid passage flow velocity；The stepped drop formation method chip of document report
It is complicated, it is necessary to realize microfluidic channel three-dimensional structure by cumbersome procedure of processing, chip production cost of manufacture is high to ask
Topic；And existing disclosure or the centrifugal drop formation micro-fluidic chip delivered, it is impossible to be applicable to magnetic particle, microballoon, list
The problems such as in the application that cell etc. is easily combined by the little particle of centrifugal forces affect, one kind is developed using centrifugation as base
The Water-In-Oil drop formation chip of plinth.
Technical scheme is as follows：
A kind of centrifugal type microfludic chip for Water-In-Oil drop formation, it is characterised in that including channel layer and closing
Layer, described channel layer include aqueous phase storage area, interface channel, drop formation area and drop collecting region, wherein, aqueous phase storage area
Position away from centrifugation shaft end is located closer to centrifugal shaft than drop storage area close to centrifugation shaft end；Drop storage area close to from
The position of spindle end is than drop formation zone position closer to centrifugal shaft；
The interface channel connection aqueous phase storage area and drop formation area, described drop formation area connection drop collecting region
With one end of interface channel；A pressure equalisation passage is also connected between aqueous phase storage area and drop collecting region；The drop formation
Angle of the opening direction axis in area with centrifugation force direction into 30-150 degree.
In use, adding the oil-phase solution of certain volume in drop collecting region, certain volume aqueous phase sample is added in aqueous phase area
Product, centrifugation, you can generate drop in drop formation area, and drop is collected automatically in drop storage area.
Preferably, angle of the opening direction axis in the drop formation area with centrifugation force direction into 45-135 degree.
Further preferably, angle of the opening direction axis in the drop formation area with centrifugation force direction into 80-100 degree.
In the present invention, drop formation area can be various shapes, such as square.But preferably, drop formation area
Structure is the wide nozzle-type structure in the narrow outlet of entrance.Bell-mouthed tilt angle ranges can be 15-165 degree.
Described sealant can sealant or lower sealant be gone up in setting or the two exists simultaneously according to being actually needed；
Described sealant is with channel layer material：Silicon, glass, quartz or organic polymer.Organic polymer for example poly- two
Methylsiloxane (polydimethylsiloxane, PDMS), polyurethane, epoxy resin, polymethyl methacrylate
(polymethyl methacrylate, PMMA), makrolon (polycarbonate, PC), cyclic olefine copolymer
(cycloolefincoplymer, COC), polystyrene (polystyrene, PS), polyethylene (polyethylene, PE), third
One or more in olefin(e) acid, rubber, fluoroplastics etc..
Described channel layer includes the main body microfluidic channel for drop formation, close by being bonded together to form with sealant
Seal passage；
Described microfluidic channel can be according to channel layer material needs, preparation method：Casting method, pressure sintering, photoetching
Polymerization and etching method；
Described pressure equalisation passage, its one end are located at drop collecting region, with drop formation offset centrifugation rotating shaft it is identical away from
From place；The other end is located at liquid phase storage area, and it is less than interface channel with the distance for centrifuging rotating shaft and centrifuges the distance of rotating shaft.
Described aqueous phase storage area volume is：0.1 μ L-1mL, stored for aqueous sample；
Described aqueous phase storage area is provided with perforate, the injection available for aqueous sample solution close to centrifugation shaft end；
Described interface channel length is 1mm-100mm, and width is 10 μm of -10mm, and depth is 1 μm of -1mm, for connecting
Aqueous phase storage area and drop formation area, allow aqueous phase solution during centrifugation smoothly to flow into drop formation area；
The shape of the interface channel can be identical with centrifugal direction, channel shape can also be adjusted according to being actually needed
It is whole；
Described drop formation area is nozzle shape opening, and entrance is narrow, and outlet is wide.Preferably arrival end width is 1 μm of -200 μ
M, nozzle exit end width are 10 μm of -1mm, and nozzle depth is 1 μm -200 μm.Described drop storage area is directly and drop formation
Area end is connected, and its depth is 1 μm of -1mm.
Described drop storage area is characterized in that described drop storage area should be full of oil phase before centrifugation generates drop
Solution；Drop collecting region depth is 1 μm -200 μm.
There is perforate described drop storage area close to centrifugation shaft end, can inject oil-phase solution or discharge oil-phase solution；
Described aqueous phase can be：Pure water, water phase buffer solution, blood, body fluid, saliva, urine and cell pyrolysis liquid etc..
Microfluidic channel carries out super-hydrophobicization processing in described channel layer.Super-hydrophobicization method can be chemical covalent knot
Legal, physisorphtion or two methods are combined.The reagent that chemical covalent method uses is fluoric silane such as dimethyloctadecylammonium
Base chlorosilane, octadecyl trichlorosilane alkane, trimethoxy propyl silane, trimethoxy propyltrichlorosilan, 1H, 1H, 2H, 2H-
Perfluoro capryl silane, 1H, 1H, 2H, 2H- perfluoro capryls dimethylchlorosilane, 1H, 1H, 2H, 2H-perfluoro capryl trichlorosilane,
1H, 1H, 2H, 2H- perfluorododecyl trichlorosilane, three perfluoro-methyl chlorosilanes, trim,ethylchlorosilane.Physisorphtion uses
Reagent solvent volatilization fluorocarbon oil such as EGC-1700, EGC-1720, EGC-1702, EGC-1704, FC-722, FC-724, FC-
725、FC-732。
In the present invention, the oil-phase solution proportion used can be more than aqueous phase, might be less that aqueous phase.It is for example, described
Oil phase is：Fluorocarbon oil (such as HFE-7100, HFE-7200, HFE-7500, HFE-71DA, HFE-71DE, HFE-72DA, HFE-72DE,
HFE-71IPA、FC-3251、FC-3252、FC-3255、FC-3275、FC-3280、FC-3283、FC-3284、FC-40、FC-
41、FC-43、FC-5311、FC-5312、FC-5320、FC-6003、FC-6064、FC-6047、FC-70、FC-72、FC-726、
FC-75, FC-77, FC-770, FC-8270, FC-84, FC-87, GH135), mineral oil or silicone oil (such as fluorosilicone, ring penta
Siloxanes, fatty radical siloxane, oligodimethylsiloxanes).To promote Water-In-Oil interface stability, workable surfactant
For：Perfluorinated surfactant, E2K0660, FS-D, FS-P, Tween and Span.
Described drop formation method main operational steps include：(1) aqueous phase is added in aqueous phase storage area, is stored in drop
Fill it up with oil phase in area；(2) centrifuge, capture and store in drop storage area automatically.
The present invention is using middle low-speed centrifugal as the driving force of drop formation, and droplet size turns with centrifugation within the specific limits
Speed is unrelated, ensures that the drop homogeneity and stability generated using the chip.This method to microfluid by leading to simultaneously
The ingehious design of road structure, the generation of droplets stable high speed is realized with a kind of single nozzle-type two dimension microfluidic channel, is overcome
The problem of stepped drop formation method is complicated, and cost of manufacture is high.
The present invention generation drop the advantages of be：1, generation is quick, convenient, the strict control without high precision sampling pump.
2, drop formation speed is fast.3, drop size is homogeneous.4 sample volumes are small, only need 2ul samples to generate drop.5. sample
Without dead volume, almost all of aqueous phase solution can generate the drop of single-size.6. due to drop formation region and centrifugation
The design that force direction forms an angle so that allow the particulate materials such as magnetic bead, microballoon, cell be present in solution.The present invention with
Other centrifugal type microfludic chips compare the analysis and application that can adapt to more unimolecules, individual cell level.7. drop is given birth to
Chip production cost of manufacture is significantly reduced into the design on the two dimension yardstick of region.8th, in the prior art, the oil phase used must
Must be bigger than aqueous phase densities, and in the present invention, the oil phase more than aqueous phase densities can be not only used, can also use and be less than aqueous phase
The oil phase of density, expands application.
Brief description of the drawings
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 depicts the chip channel configuration schematic diagram of embodiment 1.
Fig. 2 depicts the drop formation plot structure schematic diagram of embodiment 1.
Fig. 3 depicts embodiment 1PDMS chip structure schematic diagrames.
Fig. 4 depicts embodiment 2PMMA chip structure schematic diagrames.
Fig. 5 is the glass-chip structural representation of embodiment 3.
Fig. 6 is the chip channel configuration schematic diagram of embodiment 4.
Fig. 7 is the result figure of embodiment 1；
Fig. 8 is the result figure of embodiment 2
Fig. 9 is the result figure of embodiment 3
Figure 10 is the result figure of embodiment 4
1. aqueous phase storage area
2. interface channel
3. drop formation area
4. drop storage area
5. air pressure balance passage
6. nozzle shape passage
7. aqueous phase injection port
8. oil phase injection port
Sealant (glass) on 9.
10. smooth PDMS film (channel layer, PDMS)
11. the PDMS film (channel layer, PDMS) containing passage
12. lower sealant (glass)
Sealant (PMMA) on 13.
14. lower sealant (PMMA)
Sealant (glass) on 15.
16. channel layer (glass)
Embodiment
Embodiment 1PDMS materials facture of microchip and application
Referring to figs. 1 to shown in Fig. 3, chip structure includes upper sealant 9, lower sealant 12 and between upper-lower seal layer
Channel layer 10,11, channel design include aqueous phase storage area 1, interface channel 2, drop formation area 3, (the drop of drop collecting region 4
Storage area) and air pressure balance passage 5.
In the present embodiment, described upper sealant 9 and lower sealant 12 are all rectangular glass.
The material of channel layer 10 and 11 is all PDMS, and channel layer 10 is in the back of the body close to one end (in Fig. 1 for close to upper end) place
Face sets aqueous phase memory block 1, is provided with drop storage area 4 close to the back side at lower end, aqueous phase memory block 1 is extended downwardly provided with interface channel
2, the interface channel 2 is straight down.The end connection drop formation area 3 of interface channel 2, its right-hand member lateral connection interface channel
2, left end connection drop storage area 4.
In the present embodiment, channel layer 11 is flat shape.In other embodiments, channel layer 11 can also be in upper surface
If storage area.
Described air pressure balance passage 5, one end are connected to aqueous phase storage area 1, and the other end is connected to drop storage area 4.
In the present embodiment, the link position of itself and aqueous phase storage area 1 for left side topmost, and drop storage area 4 link position be and
The other side of the sustained height of drop formation area 3.
Preferably, the described volume of aqueous phase storage area 1 is：0.1 μ L-1mL, stored for aqueous sample.
In the present embodiment, the upper end that shaft end is Fig. 1 is centrifuged.Preferably, described aqueous phase storage area 1 is away from centrifugal shaft
The position at end should be located closer to centrifugal shaft than drop storage area 4 close to centrifugation shaft end；
Described aqueous phase storage area 1 is provided with perforate, the injection available for aqueous sample solution close to centrifugation shaft end；
Described interface channel size preferred length is 1mm-100mm, and width is 10 μm of -10mm, and depth is 1 μm of -1mm,
For connecting aqueous phase storage area and drop formation area, aqueous phase solution during centrifugation is set smoothly to flow into drop formation area；
The shape of the centrifugal channel can be identical with centrifugal direction, channel shape can also be adjusted according to being actually needed
It is whole；
Described drop formation area 3, preferably nozzle shape opening, entrance is narrow, and outlet is wide.Arrival end width is preferably 1 μm-
200 μm, nozzle exit end width is 10 μm of -1mm, and nozzle depth is 1 μm -200 μm.Described drop storage area is directly and drop
Generation area end is connected.Drop reservoir depth is preferably 1 μm of -1mm.
Described drop storage area should be than drop formation zone position closer to centrifugal shaft close to the position of centrifugation shaft end；
Described drop storage area should be full of oil-phase solution before centrifugation generates drop；
There is perforate described drop storage area close to centrifugation shaft end, can inject oil-phase solution or discharge oil-phase solution；
Described aqueous phase is：Pure water or water phase buffer solution.
In the present embodiment, the proportion of described oil phase is more than water.Preferably, described oil phase is：Fluorocarbon oil (such as HFE-
7100、HFE-7200、HFE-7500、HFE-71DA、HFE-71DE、HFE-72DA、HFE-72DE、HFE-71IPA、FC-3251、
FC-3252、FC-3255、FC-3275、FC-3280、FC-3283、FC-3284、FC-40、FC-41、FC-43、FC-5311、FC-
5312、FC-5320、FC-6003、FC-6064、FC-6047、FC-70、FC-72、FC-726、FC-75、FC-77、FC-770、
FC-8270, FC-84, FC-87, GH135), mineral oil or silicone oil (such as fluorosilicone, cyclopentasiloxane, fatty radical siloxane,
Oligodimethylsiloxanes).To promote Water-In-Oil interface stability, workable surfactant is：E2K0660、FS-D、FS-
P, Tween and Span.
After each layer chip prepares, 1) aqueous phase storage area sample holes are drilled through with puncher in the upper specified location of sealant 9
With drop storage area oil phase sample holes.2) it is cleaned by ultrasonic upper confining bed and bottom impermeable layer (glass material), removes surface impurity.3)
135 degree drying 1h after, be bonded after channel layer punch position be directed at upper confining bed punch position, complete covalent bond with, then general
Bottom impermeable layer is bonded, and completes the making of chip structure.4) 1720 perfluorinated solvents are injected in channel layer, 75 DEG C of drying are complete
Into the processing of channel layer perfluorinate.
4. drop formation
1) the perfluor carburetion of HFE 7500 containing 2%E2K0660 is filled it up with drop storage area by liquid-transfering gun, is stored up in aqueous phase
Deposit area and add 10 μ L PCR Mix.2) with 2800r/min, 60s pelleted by centrifugation, generate drop and stored in oil phase storage area.
Its result is shown in Fig. 7.
Implement 2PMMA material chips
Shown in the present embodiment chip reference picture 4, its basic structure and embodiment 1 are identical, except that, in the present embodiment
In, chip is only two layers up and down.Including the upper sealant 13 (PMMA materials) positioned at upper strata and the lower sealant 14 positioned at lower floor
(PMMA materials), (equally lead to wherein lower sealant 14 sets microfluidic channel structure in upper surface comprising aqueous phase storage area, connection
Road, drop formation area, drop collecting region etc.).
Specific chip manufacturing step is as follows：
1. channel layer hot padding
Hot padding is carried out to PMMA plates thick 1mm with the copper electroforming template rolled into a ball with passage, imprinting conditions are：140 degree,
600ppsi pressure, 3min hot paddings, 95 degree of demouldings, template anti-sticking layer is 1720 perfluorinated solvents.
2. the thermal bonding of confining bed and channel layer
With 105 degree, 2Mpa condition hot pressing 3min, room temperature is naturally cooled to, completes the thermal bonding of confining bed and channel layer.
3. passage surface perfluorinate is handled
1720 perfluorinated solvents are injected in channel layer, 75 DEG C of drying, is repeated 3 times, completes the processing of channel layer perfluorinate.
4. drop formation
1) the perfluor carburetion of HFE 7500 containing 2%E2K0660 is filled it up with drop storage area by liquid-transfering gun, is stored up in aqueous phase
Deposit area and add 10 μ L PCR Mix.2) with 2800r/min, 60s pelleted by centrifugation, generate drop and stored in oil phase storage area.
Its result is shown in Fig. 8.
The glass material micro-fluidic chip of embodiment 3
Shown in this chip reference picture 5, its basic structure and embodiment 1 are identical, except that, in the present embodiment, core
Piece is only two layers up and down：Upper sealant 15 (glass material) and channel layer 16 (glass material), the upper surface of channel layer 16 set miniflow
Body passage (channel design includes aqueous phase storage area, interface channel, drop formation area, drop collecting region etc.).Specific chip manufacturing
Step is as follows：
1. microfluidic channel is etched and made
1) sol evenning chromium plate is placed on one side litho machine lifting platform, and by film as on spin coating chromium plate.
2) time for exposure 8-12s is set, suction piece, lifted, contact, adherence, exposes position.(when sliding chip, film reply
Quasi- glass)
3) film is removed, sol evenning chromium plate is placed in NaOH (1.6g to 400ml) immersions 2min.--- development
4) chromium plate is taken out, ultra-pure water is cleaned, and N2 dryings, is repeated twice.
5) after drying up, ammonium ceric nitrate-perchloric acid solution (severe corrosive) is put into, layers of chrome 2min or so is corroded, to layers of chrome figure
Case corrodes complete.Clear water cleans, and drying, is repeated twice.
6) 20min or so is dried for 85 DEG C, solvent in photoresist is removed in baking.
7) chromium plate is taken to be put into mixed acid (HF 8.7ml, HNO3 16.875ml, NH3F 9.25g, total 500ml/6.9：
7.4：13.5-400ml, 40 DEG C of corrosion rate about 1.5-2um/min), heating water bath is (according to heating-up temperature and etching depth timing
Between), slightly cold, water is cleaned, micro- Microscopic observation chip surface erosion condition.(corrode width and depth is proportional, isotropism is invaded
Lose, at 40-45 DEG C of temperature, 10min etchings 15um)
8) glass-chip that upper step is handled well is put into acetone and washes away surface size, then layers of chrome is washed away with ceric ammonium nitrate solution
(fully transparent to glass, if there is layers of chrome remaining, possible photoresist is not cleaned, can be placed on acetone and wash again), is finally washed with water residual
Remaining ceric ammonium nitrate solution.
9) water cleans N after chip2Drying, separately looks for an onesize glass-chip, a layer rosin is spread on surface, by the core of experiment
Piece is pressed on rosin without surface channel, and the heater plate that two chips are put in more than 120 DEG C melts rosin.
10) glass-chip is taken out, installs card punch and perforating needle, is marked the position for needing to punch with marking pen, thereon
Uniformly one layer of ultra-pure water of sprinkling, adjustment good position punching.Ensure that intermediate layer has rosin during punching, otherwise should go heating and melting again
Rosin is uniform.
11) rosin is washed away after punching with acetone.
2. glass-chip is bonded
Channel layer is cleaned with sealant with ultra-pure water, fixed with mould, is bonded 6 hours with 650 degree in Muffle furnace, from
Taken out after being so cooled to room temperature.
3. passage surface perfluorinate is handled
1720 perfluorinated solvents are injected in channel layer, 75 DEG C of drying, is repeated 3 times, completes the processing of channel layer perfluorinate.
4. drop formation
1) the perfluor carburetion of HFE 7500 containing 2%E2K0660 is filled it up with drop storage area by liquid-transfering gun, is stored up in aqueous phase
Deposit area and add 10 μ L PCR Mix.2) with 2800r/min, 60s pelleted by centrifugation, generate drop and stored in oil phase storage area.
Its result is shown in Fig. 9.
Embodiment 4
Referring to Fig. 6, the basic structure and embodiment 1 of the present embodiment are identical, except that, in the present embodiment, drop
The latter half (position under drop formation area 3, Fig. 6 in A portions) of storage area 4 is longer, and the oil phase used is to compare aqueous phase densities
Lighter light mineral oil, the surfactant used are 5%Span 80.
Its result is shown in Figure 10.
Claims (10)
- A kind of 1. centrifugal type microfludic chip for Water-In-Oil drop formation, it is characterised in that including channel layer and confining bed, Described channel layer includes aqueous phase storage area, interface channel, drop formation area and drop collecting region, wherein, aqueous phase storage area is remote The position of luxuriant spindle end is located closer to centrifugal shaft than drop storage area close to centrifugation shaft end；Drop storage area is close to centrifugation The position of shaft end is than drop formation zone position closer to centrifugal shaft；The interface channel connection aqueous phase storage area and drop formation area, described drop formation area connection drop collecting region and company Connect the one end in road；A pressure equalisation passage is also connected between aqueous phase storage area and drop collecting region；The drop formation area Angle of the opening direction axis with centrifugation force direction into 30-150 degree.
- A kind of 2. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that institute State angle of the opening direction axis in drop formation area with centrifugation force direction into 45-135 degree.
- A kind of 3. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that institute State angle of the opening direction axis in drop formation area with centrifugation force direction into 80-100 degree.
- 4. a kind of centrifugal type microfludic chip for Water-In-Oil drop formation as described in any one of claims 1 to 3, it is special Sign is that the structure in drop formation area is the wide nozzle-type structure in the narrow outlet of entrance.
- A kind of 5. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 4, it is characterised in that spray Mouth arrival end width is 1 μm -200 μm, and nozzle exit end width is 10 μm of -1mm, and nozzle depth is 1 μm -200 μm.
- A kind of 6. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that institute The pressure equalisation passage stated, its one end are located at drop collecting region, and drop formation offset centrifugation rotating shaft same distance；The other end Positioned at liquid phase storage area, it is less than interface channel with the distance for centrifuging rotating shaft and centrifuges the distance of rotating shaft.
- 7. a kind of centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that described Aqueous phase storage area volume is 0.1 μ L-1mL.
- A kind of 8. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that connection Passage length is 1mm-100mm, and width is 10 μm of -10mm, and depth is 1 μm of -1mm.
- A kind of 9. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that drop Collecting region depth is 1 μm -500 μm.
- A kind of 10. centrifugal type microfludic chip for Water-In-Oil drop formation as claimed in claim 1, it is characterised in that oil The liquor ratio that mixes is great in aqueous phase solution, or less than aqueous phase solution.
Priority Applications (1)
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| CN109046484A (en) * | 2018-09-12 | 2018-12-21 | 上海交通大学 | A kind of method that displacement-type micro-fluidic chip generates drop by surface tension |
| CN109225362A (en) * | 2018-08-30 | 2019-01-18 | 中国科学院上海微系统与信息技术研究所 | A kind of centrifugal drop micro-fluidic chip |
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| CN111635487A (en) * | 2020-05-01 | 2020-09-08 | 浙江大学 | Photocuring oil phase for preparing photocuring liquid drop array chip, and preparation method, product and application of photocuring liquid drop array chip |
| WO2020177088A1 (en) * | 2019-03-01 | 2020-09-10 | 深圳市博瑞生物科技有限公司 | Microfluidic chip |
| CN115007236A (en) * | 2022-06-06 | 2022-09-06 | 湖北大学 | Portable liquid drop manufacturing device based on centrifugal oscillation and liquid drop manufacturing method thereof |
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Application publication date: 20171208 |