CN107434853A - A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces - Google Patents
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces Download PDFInfo
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- CN107434853A CN107434853A CN201710571664.7A CN201710571664A CN107434853A CN 107434853 A CN107434853 A CN 107434853A CN 201710571664 A CN201710571664 A CN 201710571664A CN 107434853 A CN107434853 A CN 107434853A
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- oxygen
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- super hydrophilic
- cell culturing
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
- C08J7/123—Treatment by wave energy or particle radiation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
- C08J2325/02—Homopolymers or copolymers of hydrocarbons
- C08J2325/04—Homopolymers or copolymers of styrene
- C08J2325/06—Polystyrene
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, including microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation active particle；Mixed gas includes the following composition based on weight/mass percentage composition：O290 95%, H21 5%, N21 5%, N3F 1 5%；Plasma treatment step：The active particle that microwave treatment step is obtained, which is passed through to be placed with the plasma generator of polystyrene base material, carries out plasma treatment, obtains oxygen-enriched super hydrophilic cell culturing surfaces.The cell culturing surfaces that the method for the present invention is prepared have super hydrophilic effect persistently, stable, containing abundant oxygen-containing functional group, meanwhile, it also has the characteristics of adherence rate is high, cell throughput rate is high and cellular morphology is good.
Description
Technical field
The present invention relates to a kind of preparation method of cell culturing surfaces, and in particular to a kind of oxygen-enriched super hydrophilic cell culture table
The preparation method in face.
Background technology
The main method of macromolecule material surface modification includes surface cladding process, surface oxidation method, blending surface is modified, etc.
Processes and surface grafting method etc., wherein plasma method include atmospheric plasma method, vacuum plasma method and corona etc. again
Ion method, for these plasma methods provide the energy source include normal pressure corona discharge, vacuum plasma method radio frequency,
Direct current electric discharge, low frequency, radio frequency, microwave method and other electromagnetic radiation methods.
In a variety of alternative surface modification technologies, plasma technique, particularly low temperature plasma skill
Art is a kind of more satisfactory process technology, and this technology has normal temperature work, in stable condition, is handled uniform, pollution-free etc. excellent
Point, high ionization degree, the plasma of high activity can be particularly provided, be widely used in handling the surface of various materials,
Play the role of in terms of the industry such as electronics, machinery, plastics, rubber and biomedical engineering important.It is presently used at the modification of surface
The plasma system of reason mainly uses three frequency ranges, and the radio frequency of low frequency, 13.56MHz less than 100KHz and 2.45GHz's is micro-
Ripple, wherein radio frequency and microwave are the most commonly used.Now, plasma has been easier to control in technique, and environmental pollution is small,
And as life science cell culture series consumptive material, be mainly process using polystyrene material, but
This material surface is nonpolar, and hydrophily is very poor, is unfavorable for the adherent growth of stem cell.Therefore, people are adherent thin in culture
Born of the same parents usually can be modified or handle to culture surface before.But directly using plasma process for treating surface is to polyphenyl second
Alkene is modified the water-wetted surface of processing formation, and its surface hydrophilic index can not meet the harsh difficulty of those growth conditions with performance
Support requirement of the adherent growth of cell and those cells cultivated in the insufficient environment of nutritional condition to material hydrophilic.It is existing
There are the following problems for the Surface Treatment with Plasma method for having in technology：The product surface oxy radical of processing is insufficient, cell training
Foster speed of production, adherent rate and survival rate be not high to be devoted a tremendous amount of time, energy and expense, so as to cause condition of culture difficult
Trouble is waited in control.
The content of the invention
For overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of oxygen-enriched super hydrophilic cell culturing surfaces
Preparation method, the cell culturing surfaces being prepared using this method have super hydrophilic effect persistently, stable, containing abundant
Oxygen-containing functional group, meanwhile, it also has the characteristics of adherence rate is high, cell throughput rate is high and cellular morphology is good.
The purpose of the present invention adopts the following technical scheme that realization：
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, it is characterised in that including：
Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation activity
Particle；The mixed gas includes the following composition based on weight/mass percentage composition：O290-95%, H21-5%, N21-5%,
N3F 1-5%；
Plasma treatment step：The active particle that microwave treatment step is obtained be passed through be placed with polystyrene base material etc.
Plasma treatment is carried out in ion generator, obtains oxygen-enriched super hydrophilic cell culturing surfaces.
Further, in microwave treatment step, microwave frequency 2440-2470MHZ.
Further, in microwave treatment step, microwave power 6kw, processing time is the 50-150 seconds.
Further, in microwave treatment step, processing time is 100 seconds.
Further, in microwave treatment step, the mixed gas includes the following composition based on weight/mass percentage composition：O2
90-95%, H21-2%, N21-3%, N3F 3-5%.
Further, in microwave treatment step, the mixed gas includes the following composition based on weight/mass percentage composition：O2
95%th, H21%th, N21%th, N3F 3%.
Further, in plasma treatment step, specific treatment conditions are as follows：Vacuum is 1.5-10mT, temperature 28-
32 °, gas flow 100-300sccm, processing power 100-500w, processing time 20-40s.
Further, in plasma treatment step, specific treatment conditions are as follows：Vacuum is 5mT, temperature is 28 °, gas
Flow is 300sccm, processing power 500w, processing time 40s.
Further, in plasma treatment step, active particle is passed through in plasma generator by sliding vane rotary pump.
Further, microwave treatment step and plasma treatment step are completed in microwave plasma integrated equipment.
Compared with prior art, the beneficial effects of the present invention are：
The present invention by microwave generator to reacting gas, at oxygen, nitrogen, hydrogen and nitrogen trifluoride mixture
Reason, is ionized into high activity electronics, ion and molecule isoreactivity particle, then is passed through in plasma generator by active particle
Row plasma treatment, substantial amounts of O+、O-、O2+、O2-、O、O3, ozone ion, the particle such as meta-stable O2 and free electron just and polyphenyl
Physical and chemical reaction occurs for the surface of ethene base material, so as to produce substantial amounts of polar group on the surface of polystyrene base material,
And directly improve the hydrophily on surface.Tested by surface contact angle, before base material treatment be about 90 °, be down to after processing 10 ° or with
Under, so that in a kind of oxygen-enriched superhydrophilic surface of plastic basis material surface forming, verified by contrasting cell culture, by this work
Skill processing cell culture consumptive material, adherence rate, cell throughput rate, cellular morphology significantly better than directly using commonly etc.
Gas ions handle product.
Embodiment
Below, with reference to embodiment, the present invention is described further, it is necessary to which explanation is, what is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.This reality
Apply employed in example to material be commercially available.
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, including：
Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation activity
Particle；Mixed gas includes the following composition based on weight/mass percentage composition：O290-95%, H21-5%, N21-5%, N3F
1-5%；
Plasma treatment step：The active particle that microwave treatment step is obtained be passed through be placed with polystyrene base material etc.
Plasma treatment is carried out in ion generator, obtains oxygen-enriched super hydrophilic cell culturing surfaces.
As preferred embodiment, in microwave treatment step, microwave frequency 2440-2470MHZ.
As preferred embodiment, in microwave treatment step, microwave power 6kw, processing time is the 50-150 seconds.
As preferred embodiment, in microwave treatment step, processing time is 100 seconds.
As preferred embodiment, in microwave treatment step, mixed gas includes as follows based on weight/mass percentage composition
Composition：O290-95%, H21-2%, N21-3%, N3F 3-5%.
As preferred embodiment, in microwave treatment step, mixed gas includes as follows based on weight/mass percentage composition
Composition：O295%th, H21%th, N21%th, N3F 3%.
As preferred embodiment, in plasma treatment step, specific treatment conditions are as follows：Vacuum is 1.5-
10mT, temperature are 28-32 °, gas flow 100-300sccm, processing power 100-500w, processing time 20-40s.
As preferred embodiment, in plasma treatment step, specific treatment conditions are as follows：Vacuum is 5mT, temperature
For 28 °, gas flow 300sccm, processing power 500w, processing time 40s.
As preferred embodiment, in plasma treatment step, active particle is passed through by plasma by sliding vane rotary pump and sent out
In raw device.
As preferred embodiment, microwave treatment step and plasma treatment step are in microwave plasma integrated equipment
Middle completion.The equipment that microwave plasma integrated equipment preferably is selected from the model AX2600 of machine instrument company (MKS) of the U.S. ten thousand, it is micro-
Wave power is 6kw.
Embodiment 1：
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, including：
Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation activity
Particle；Mixed gas includes the following composition based on weight/mass percentage composition：O290%th, H22%th, N23%th, N3F 5%；Microwave
Frequency is 2440MHZ, and microwave power 6kw, processing time is 100 seconds.
Plasma treatment step：The active particle that microwave treatment step is obtained be passed through be placed with polystyrene base material etc.
Plasma treatment is carried out in ion generator so that the surface of polystyrene basic unit produces abundant oxygen-containing functional group, so as to shape
Into oxygen-enriched super hydrophilic cell growth surface.Specific treatment conditions are as follows：Vacuum is 10mT, temperature is 32 °, gas flow is
100sccm, processing power 100w, processing time 20s.
The finished product of the present embodiment is detected by x-ray photoelectron spectroscopy, the oxygen member of treated polystyrene base material
Element is increased to 32.5% by original 4.6%, and this explanation introduces substantial amounts of oxygen-containing on polystyrene base material surface after treatment
Group；Meanwhile determined using contact angle measurement, its hydrophilic cell growth surface is 10 ° to the contact angle of water, have persistently,
Stable super hydrophilic effect.
Embodiment 2：
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, including：
Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation activity
Particle；Mixed gas includes the following composition based on weight/mass percentage composition：O292%th, H21%th, N22%th, N3F 5%；Microwave
Frequency is 2450MHZ, and microwave power 6kw, processing time is 100 seconds.
Plasma treatment step：The active particle that microwave treatment step is obtained be passed through be placed with polystyrene base material etc.
Plasma treatment is carried out in ion generator so that the surface of polystyrene basic unit produces abundant oxygen-containing functional group, so as to shape
Into oxygen-enriched super hydrophilic cell growth surface.Specific treatment conditions are as follows：Vacuum is 8mT, temperature is 30 °, gas flow is
200sccm, processing power 200w, processing time 30s.
The finished product of the present embodiment is detected by x-ray photoelectron spectroscopy, the oxygen member of treated polystyrene base material
Element is increased to 35% by original 4.6%, and this explanation introduces substantial amounts of oxygen-containing group on polystyrene base material surface after treatment
Group；Meanwhile determined using contact angle measurement, its hydrophilic cell growth surface is 9 ° to the contact angle of water, is had persistently, stably
Super hydrophilic effect.
Embodiment 3：
A kind of preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, including：
Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation activity
Particle；Mixed gas includes the following composition based on weight/mass percentage composition：O295%th, H21%th, N21%th, N3F 3%；Microwave
Frequency is 2470MHZ, and microwave power 6kw, processing time is 100 seconds.
Plasma treatment step：The active particle that microwave treatment step is obtained be passed through be placed with polystyrene base material etc.
Plasma treatment is carried out in ion generator so that the surface of polystyrene basic unit produces abundant oxygen-containing functional group, so as to shape
Into oxygen-enriched super hydrophilic cell growth surface.Specific treatment conditions are as follows：Vacuum is 5mT, temperature is 28 °, gas flow is
300sccm, processing power 500w, processing time 40s.
The finished product of the present embodiment is detected by x-ray photoelectron spectroscopy, the oxygen member of treated polystyrene base material
Element is increased to 40% by original 4.6%, and this explanation introduces substantial amounts of oxygen-containing group on polystyrene base material surface after treatment
Group；Meanwhile determined using contact angle measurement, its hydrophilic cell growth surface is 8 ° to the contact angle of water, is had persistently, stably
Super hydrophilic effect.
Above-mentioned embodiment is only the preferred embodiment of the present invention, it is impossible to the scope of protection of the invention is limited with this,
The change and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed scope.
Claims (10)
- A kind of 1. preparation method of oxygen-enriched super hydrophilic cell culturing surfaces, it is characterised in that including：Microwave treatment step：Mixed gas is passed through progress microwave discharge processing in microwave generator, ionization generation active particle； The mixed gas includes the following composition based on weight/mass percentage composition：O290-95%, H21-5%, N21-5%, N3F 1- 5%；Plasma treatment step：The active particle that microwave treatment step is obtained is passed through the plasma for being placed with polystyrene base material Plasma treatment is carried out in generator, obtains oxygen-enriched super hydrophilic cell culturing surfaces.
- 2. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that microwave treatment walks In rapid, microwave frequency 2440-2470MHZ.
- 3. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that microwave treatment walks In rapid, microwave power 6kw, processing time is the 50-150 seconds.
- 4. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 3, it is characterised in that microwave treatment walks In rapid, processing time is 100 seconds.
- 5. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that microwave treatment walks In rapid, the mixed gas includes the following composition based on weight/mass percentage composition：O290-95%, H21-2%, N21-3%, N3F 3-5%.
- 6. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 5, it is characterised in that microwave treatment walks In rapid, the mixed gas includes the following composition based on weight/mass percentage composition：O295%th, H21%th, N21%th, N3F 3%.
- 7. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that plasma treatment In step, specific treatment conditions are as follows：Vacuum is 1.5-10mT, temperature is 28-32 °, gas flow 100-300sccm, Processing power is 100-500w, processing time 20-40s.
- 8. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 7, it is characterised in that plasma treatment In step, specific treatment conditions are as follows：Vacuum is 5mT, temperature is 28 °, gas flow 300sccm, processing power are 500w, processing time 40s.
- 9. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that plasma treatment In step, active particle is passed through in plasma generator by sliding vane rotary pump.
- 10. the preparation method of oxygen-enriched super hydrophilic cell culturing surfaces as claimed in claim 1, it is characterised in that microwave treatment Step and plasma treatment step are completed in microwave plasma integrated equipment.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110922166A (en) * | 2019-11-08 | 2020-03-27 | 安徽大学 | High-corrosion-resistance composite material and preparation method thereof |
| CN111286066A (en) * | 2020-02-28 | 2020-06-16 | 广州洁特生物过滤股份有限公司 | Preparation process of substrate hydrophilic surface and three-dimensional cell culture support |
| CN112851995A (en) * | 2021-01-29 | 2021-05-28 | 苏州赛普生物科技有限公司 | Surface hydrophilic treatment method for cell culture dish |
| CN114409947A (en) * | 2021-12-31 | 2022-04-29 | 洛阳富道生物科技有限公司 | Surface modification method of plastic product |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110922166A (en) * | 2019-11-08 | 2020-03-27 | 安徽大学 | High-corrosion-resistance composite material and preparation method thereof |
| CN111286066A (en) * | 2020-02-28 | 2020-06-16 | 广州洁特生物过滤股份有限公司 | Preparation process of substrate hydrophilic surface and three-dimensional cell culture support |
| WO2021169590A1 (en) * | 2020-02-28 | 2021-09-02 | 广州洁特生物过滤股份有限公司 | Preparation process for hydrophilic surface of substrate and three-dimensional cell culture scaffold |
| CN112851995A (en) * | 2021-01-29 | 2021-05-28 | 苏州赛普生物科技有限公司 | Surface hydrophilic treatment method for cell culture dish |
| CN114409947A (en) * | 2021-12-31 | 2022-04-29 | 洛阳富道生物科技有限公司 | Surface modification method of plastic product |
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