CN104788707A - Preparation method of super-hydrophilic cell growth surface - Google Patents
Preparation method of super-hydrophilic cell growth surface Download PDFInfo
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- CN104788707A CN104788707A CN201510194152.4A CN201510194152A CN104788707A CN 104788707 A CN104788707 A CN 104788707A CN 201510194152 A CN201510194152 A CN 201510194152A CN 104788707 A CN104788707 A CN 104788707A
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Abstract
The invention discloses a preparation method of a super-hydrophilic cell growth surface. The preparation method sequentially comprises the following steps: 1) preprocessing, to be specific, mixed gases of Ar and O2 are introduced into a plasma reaction chamber, a polystyrene substrate is subjected to plasma etching treatment; 2) an acrylic monomer is applied on the surface of the preprocessed polystyrene substrate in a spraying manner, so that the surface of the polystyrene substrate is grafted with the acrylic monomer, and the hydrophilic cell growth surface is formed. The super-hydrophilic cell growth surface has a lasting stable super-hydrophilic effect, and the super-hydrophilic effect is measured by a contact angle meter to be less than 10 degrees. Besides, the material physical property of a polystyrene plastic is not changed, the biological property for cell culture is met, and the high transparency of the polystyrene substrate is guaranteed.
Description
Technical field
The present invention relates to a kind of cell culture apparatus, be specifically related to a kind of preparation method of super hydrophilic cell growth surface.
Background technology
Along with the development of modern science, especially biomedical rapid advances, stem cell is cultivated becomes basic work very important in a lot of Study and appliance field.All the time, people generally adopt the culture plate of polystyrene material or culturing bottle as the instrument of cell cultures.Polystyrene is a kind of water white thermoplastics, is generally nontoxic, odorless, colourless transparent grain, and like glassy hard brittle material, general formula is [(CH
2cHC
6h
5) n], English name is Polystyrene, is called for short PS.Its goods have superior water resistant, moistureproofness.In the productive life of people, the purposes of polystyrene is very extensive, is a large purposes wherein as cell cultures.
The cell culturing surfaces of polystyrene material is nonpolar, and wetting ability is very poor, is unfavorable for the adherent growth of stem cell.Therefore, people usually can carry out modification or process to culture surface before culturing stem cells.And directly using plasma process for treating surface p-poly-phenyl ethene carries out the water-wetted surface of modification formation, the difficulty that its surface hydrophilic index and performance can not meet those growth conditions harshnesses supports the adherent growth of cell and those cultured cells in the environment that nutritional condition is insufficient to the requirement of material hydrophilic.The good adherent growth of above-mentioned cell needs the apparent index of wetting ability to reach 10 degree (contact angles) below, and the apparent index of wetting ability that plasma surface treatment technology reaches is 60 degree (contact angles).
Summary of the invention
For overcoming the defect of prior art, the object of the present invention is to provide a kind of preparation method of super hydrophilic cell growth surface, cell growth surface prepared by this preparation method has lasting, stable super hydrophilic effect, and super hydrophilic effect is measured lower than 10 ° by contact angle measurement.Do not change again the material physical property of polystyrene plastic and meet the biological property of cell cultures simultaneously, ensureing the high transparency performance of polystyrene base material.
Realize object of the present invention to reach by taking following technical scheme:
A preparation method for super hydrophilic cell growth surface, is characterized in that, comprise the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 20-150mT, under temperature is 30-55 °, gas flow is 5-80sccm, processing power is 60-350w, the treatment time is the condition of 100-1200s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 20-150mT in vacuum tightness, under temperature is 30-55 °, gas flow is 20-180sccm, processing power is 60-350w, the treatment time is the condition of 100-1200s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
As preferably, the contact angle of described hydrophilic cell growth surface to water is less than 10 degree.
Beneficial effect of the present invention is:
1, the present invention is by adopting the continuous super hydrophilic technique of disposable two-step approach, under given conditions, first p-poly-phenyl ethylene-based layer carries out plasma etch process, the surface of polystyrene basic unit is made to form concaveconvex structure, increase contact area, while polystyrene surface introduces coarse structure, introduce a large amount of polar groups, make polystyrene base material surface possess certain wetting ability.But these polar groups are in very unstable high-energy state, As time goes on, polar group reduces gradually, and hydrophilicity reduces gradually and even disappears.Therefore, Acrylic Acid Monomer puts on through pretreated polystyrene basic unit surface with the form of spraying by the present invention, under given conditions, make the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form hydrophilic cell growth surface, make it reach leading in the world and have lasting, stable super hydrophilic effect, super hydrophilic effect is measured lower than 10 ° by contact angle measurement.Do not change again the material physical property of polystyrene plastic and meet the biological property of cell cultures simultaneously, and ensureing the high transparency performance of polystyrene base material.The present invention is by high-energy electron radio frequency source, and vacuum chamber and Ar/O2 mixed gas integrate, and to produce highly active plasma, utilizes acrylic graft-modified process polystyrene.The substantial change that process occurs is the aromatic hydrocarbon functional group quantity considerably reducing polystyrene surface, and has increased considerably the quantity of oxygen-containing functional group in polystyrene chain.So both ensure that polystyrene material body phase performance is constant, obtain stable superhydrophilic surface's modified effect simultaneously.
2, mixed gas is applied in animal cell culture device at ultralow vacuum tightness (about 20mT), low temperature (about 30 degree), plasma technique p-poly-phenyl vinyl plastics surface initiation grafting Acrylic Acid Monomer modification technology by the present invention, cell culture apparatus surface adds the oxygen level using polystyrene as substrate surface, improve cell culture apparatus surface hydrophilicity and wettability, and then be conducive to adherent growth and the amplification of cell.Those are made difficult to support cells and the barren cell of nutritional condition also can realize good adherent growth.This ultra-hydrophilic surface is particularly suitable for those needs long-time adherent production and low serum free culture system environment could realize the cell type of cellular products high production.
Embodiment
Below by way of preferred embodiment, the present invention is expressed in further detail, but the present invention is not limited in following embodiment.
Embodiment 1:
A preparation method for super hydrophilic cell growth surface, comprises the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 20mT, under temperature is 30 °, gas flow is 10sccm, processing power is 350w, the treatment time is the condition of 1100s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 20mT in vacuum tightness, under temperature is 30 °, gas flow is 20sccm, processing power is 50w, the treatment time is the condition of 1200s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
Finished product described in the present embodiment is measured by contact angle measurement, and its hydrophilic cell growth surface is 8.5 ° to the contact angle of water, has lasting, stable super hydrophilic effect.
Embodiment 2:
A preparation method for super hydrophilic cell growth surface, comprises the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 50mT, under temperature is 30 °, gas flow is 5sccm, processing power is 60w, the treatment time is the condition of 100s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 20T in vacuum tightness, under temperature is 30 °, gas flow is 20sccm, processing power is 60w, the treatment time is the condition of 100s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
Finished product described in the present embodiment is measured by contact angle measurement, and its hydrophilic cell growth surface is 9.9 ° to the contact angle of water, has lasting, stable super hydrophilic effect.
Embodiment 3:
A preparation method for super hydrophilic cell growth surface, comprises the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 30mT, under temperature is 35 °, gas flow is 20sccm, processing power is 80w, the treatment time is the condition of 80s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 50mT in vacuum tightness, under temperature is 35 °, gas flow is 80sccm, processing power is 120w, the treatment time is the condition of 500s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
Finished product described in the present embodiment is measured by contact angle measurement, and its hydrophilic cell growth surface is 9.2 ° to the contact angle of water, has lasting, stable super hydrophilic effect.
Embodiment 4:
A preparation method for super hydrophilic cell growth surface, comprises the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 50mT, under temperature is 40 °, gas flow is 20sccm, processing power is 150w, the treatment time is the condition of 1000s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, vacuum tightness be 120mT, under temperature is 50 °, gas flow is 150sccm, processing power is 200w, the treatment time is the condition of 1000s, make the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form hydrophilic cell growth surface, obtain finished product.
Finished product described in the present embodiment is measured by contact angle measurement, and its hydrophilic cell growth surface is 9 ° to the contact angle of water, has lasting, stable super hydrophilic effect.
Embodiment 5:
A preparation method for super hydrophilic cell growth surface, comprises the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 150mT, under temperature is 55 °, gas flow is 80sccm, processing power is 350w, the treatment time is the condition of 1200s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 150mT in vacuum tightness, under temperature is 55 °, gas flow is 180sccm, processing power is 350w, the treatment time is the condition of 1200s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
Finished product described in the present embodiment is measured by contact angle measurement, and its hydrophilic cell growth surface is 9.8 ° to the contact angle of water, has lasting, stable super hydrophilic effect.
For a person skilled in the art, according to technical scheme described above and design, other various corresponding change and distortion can be made, and all these change and distortion all should belong within the protection domain of the claims in the present invention.
Claims (2)
1. a preparation method for super hydrophilic cell growth surface, is characterized in that, comprise the following steps successively:
1) pre-treatment: first by Ar and O
2mixed gas passes in plasma-reaction-chamber, vacuum tightness be 20-150mT, under temperature is 30-55 °, gas flow is 5-80sccm, processing power is 60-350w, the treatment time is the condition of 100-1200s, p-poly-phenyl ethylene-based layer carries out plasma etch process, makes the surface of polystyrene basic unit form concaveconvex structure;
2) graft modification process: Acrylic Acid Monomer is put on through step 1 with the form of spraying) pretreated polystyrene basic unit surface, be 20-150mT in vacuum tightness, under temperature is 30-55 °, gas flow is 20-180sccm, processing power is 60-350w, the treatment time is the condition of 100-1200s, makes the surface grafting Acrylic Acid Monomer of polystyrene basic unit, form super hydrophilic cell growth surface.
2. the preparation method of super hydrophilic cell growth surface according to claim 1, is characterized in that: the contact angle of described hydrophilic cell growth surface to water is less than 10 degree.
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| CN105219644A (en) * | 2015-11-13 | 2016-01-06 | 广州洁特生物过滤股份有限公司 | Responsive to temperature type cell culturing surfaces and preparation method thereof |
| CN108998377A (en) * | 2018-08-13 | 2018-12-14 | 太原市罗塞塔石生物技术有限公司 | A kind of cardiac muscle cell and nerve cell special culture dish |
| CN111171360A (en) * | 2020-02-28 | 2020-05-19 | 广州洁特生物过滤股份有限公司 | Cell culture apparatus surface modification method and cell culture apparatus |
| CN111320779A (en) * | 2020-02-28 | 2020-06-23 | 广州洁特生物过滤股份有限公司 | Method for modifying surface of substrate and cell culture apparatus |
| CN112480750A (en) * | 2020-12-03 | 2021-03-12 | 武汉中科先进技术研究院有限公司 | Super-hydrophilic coating for cell culture and preparation method thereof |
| CN114107164A (en) * | 2021-11-02 | 2022-03-01 | 武汉中科先进技术研究院有限公司 | Preparation method of surface for adherent growth of cells in serum-free environment |
| CN116005445A (en) * | 2023-02-09 | 2023-04-25 | 上海乐纯生物技术有限公司 | Polymer material surface treatment method for promoting cell adhesion |
| CN116180451A (en) * | 2023-03-01 | 2023-05-30 | 湖南泽睿新材料有限公司 | Continuous silicon carbide fiber sizing agent and application thereof |
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| US11286318B2 (en) | 2015-11-13 | 2022-03-29 | Guangzhou Jet Bio-Filtration Co., Ltd. | Temperature sensitive cell culture surface and preparation method thereof |
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| WO2021169599A1 (en) * | 2020-02-28 | 2021-09-02 | 广州洁特生物过滤股份有限公司 | Substrate surface modification method and cell culture apparatus |
| WO2021169600A1 (en) * | 2020-02-28 | 2021-09-02 | 广州洁特生物过滤股份有限公司 | Surface modification method for cell culture apparatus, and cell culture apparatus |
| CN111320779A (en) * | 2020-02-28 | 2020-06-23 | 广州洁特生物过滤股份有限公司 | Method for modifying surface of substrate and cell culture apparatus |
| CN112480750A (en) * | 2020-12-03 | 2021-03-12 | 武汉中科先进技术研究院有限公司 | Super-hydrophilic coating for cell culture and preparation method thereof |
| CN114107164A (en) * | 2021-11-02 | 2022-03-01 | 武汉中科先进技术研究院有限公司 | Preparation method of surface for adherent growth of cells in serum-free environment |
| CN114107164B (en) * | 2021-11-02 | 2024-04-30 | 武汉中科先进材料科技有限公司 | Preparation method of surface for cell wall-attached growth under serum-free condition |
| CN116005445A (en) * | 2023-02-09 | 2023-04-25 | 上海乐纯生物技术有限公司 | Polymer material surface treatment method for promoting cell adhesion |
| CN116005445B (en) * | 2023-02-09 | 2024-06-21 | 上海乐纯生物技术股份有限公司 | Polymer material surface treatment method for promoting cell adhesion |
| CN116180451A (en) * | 2023-03-01 | 2023-05-30 | 湖南泽睿新材料有限公司 | Continuous silicon carbide fiber sizing agent and application thereof |
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Application publication date: 20150722 |