CN107432244A

CN107432244A - A kind of method using corona treatment sunflower

Info

Publication number: CN107432244A
Application number: CN201610355002.1A
Authority: CN
Inventors: 王立言; 毕鲜荣
Original assignee: Jiaxing Skywood Biological Technology Co Ltd
Current assignee: Jiaxing Skywood Biological Technology Co Ltd
Priority date: 2016-05-25
Filing date: 2016-05-25
Publication date: 2017-12-05

Abstract

The present invention provides a kind of method using corona treatment sunflower, from the ornamental flower sunflower generally planted as vegetable material, using plasma irradiating sunflower sample, including sunflower seeds, sunflower young shoot and Sunflower Seedlings, sample is set to be modified.Plasma used in this method is caused by under the conditions of atmospheric pressure at room, need not be by means of harsh environments such as vacuum, high pressures, simultaneously, the method for changing plant structural gene with DNA restructuring, cell fusion etc. is entirely different, this method is physical modification, and security is very good and operation is simple.

Description

A kind of method using corona treatment sunflower

Technical field

The invention belongs to the application field of plasma technique, more particularly to a kind of method using corona treatment sunflower.

Background technology

At present, non-equilibrium cold plasma can be produced under low air pressure condition, but needs to complete in the vacuum chamber, it is impossible to realizes continuous operation, simultaneously because vacuum chamber volume is larger, builds and maintenance cost is expensive, significantly limit its application.Currently under atmospheric conditions can caused by plasma have two kinds, one kind is hot plasma, the plasma as caused by electric arc, temperature about 10000K magnitudes, and for fearing hot material, such as microbial material and vegetable material, its application is restricted；Another kind is cold plasma, the plasma as caused by corona discharge, dielectric barrier discharge, temperature near room temperature, suitable for the processing of biomaterial.

Cold plasma existing more research in terms of microbial cell processing, as CN103710335B discloses " a kind of method that microorganism competent is prepared using atmospheric pressure at room plasma ", bacterial cell suspension, actinomycetes cells suspension, fungal cell's suspension, alga cells suspension or algae protoplasm somatocyte suspension can be handled；CN103911363A discloses " a kind of method and device of mutagenic treatment biomaterial ", can handle the cell being coated with solid medium；CN204848891U discloses " a kind of plasma breeding equipment of mutagenic treatment anaerobe ", and plasma mutagenesis is combined with vacuumizing, realizes under anaerobic condition to the plasma mutagenic treatment of anaerobe.

Plasma is atmospheric pressure at room plasma (Atmospheric and Room Temperature Plasma used by mentioned microorganism cellular processes, ARTP), also there is the method being modified using dielectric barrier discharge to plant in existing research, as CN1685807A is disclosed " a kind of plant method of modifying ".However, atmospheric pressure at room plasma due to eliminating dielectric overlay, discharges more uniform compared with dielectric impedance, and generator architecture is simple, cost is low, the temperature near room temperature of produced plasma jet, and ozone concentration and ultraviolet radiation intensity are very low, therefore applicability is stronger.

To expand application of the atmospheric pressure at room plasma in plant treatment field, a kind of effective method for utilizing atmospheric pressure at room corona treatment vegetable material is needed badly.

The content of the invention

The present invention is from the ornamental flower sunflower generally planted as vegetable material, purpose is to provide a kind of method using corona treatment sunflower, to obtain the excellent Sunflower Varieties of character, application of the atmospheric pressure at room plasma in plant treatment field is expanded.

Technical scheme is as follows：

A kind of method using corona treatment sunflower, it is characterised in that comprise the following steps：

(1) make sunflower sample dispersion that culture dish be positioned over to the underface of plasma jet in culture dish；

(2) the sunflower sample in plasma irradiating culture dish is utilized, the time of the plasma irradiating is 10s~60min；

(3) sunflower sample is taken out to be cultivated.

The sunflower sample includes sunflower seeds, sunflower young shoot and Sunflower Seedlings.

When the sunflower sample is sunflower young shoot or Sunflower Seedlings, sterilized water is added in the culture dish.

Preferably, the plasma is atmospheric pressure at room plasma, generation of being discharged by bare metal electrode under normal pressure, room temperature condition.

The atmospheric pressure at room plasma produces by source of the gas of helium, argon gas, nitrogen, oxygen, air or its mixed gas.

Preferably, the atmospheric pressure at room plasma produces by source of the gas of helium.

The throughput of the atmospheric pressure at room plasma is 2~20L/min, preferably 10~15L/min.

It is further preferred that the time of plasma irradiating described in step (2) is 20s~30min.

Beneficial effect：A kind of method using corona treatment sunflower, selected plasma be discharged as bare metal electrode under normal pressure, room temperature condition caused by atmospheric pressure at room plasma, the energetic ions such as the helium atom of excitation state, oxygen atom, nitrogen-atoms, OH free radicals, it can be interacted with the inhereditary material in sunflower, excite the character of sunflower to change.

This method need not by means of harsh environments such as vacuum, high pressures, meanwhile, the method for changing plant structural gene with DNA restructuring, cell fusion etc. is entirely different, and this method is physical modification, and security is very good and step is simple and convenient to operate.

Brief description of the drawings

Fig. 1 is the atmospheric pressure at room plasma mutation breeding instrument schematic diagram of the embodiment of the present invention.

Fig. 2 is the processing system schematic diagram in the mutation breeding equipment of the embodiment of the present invention.

Fig. 3 is the upgrowth situation figure of the sunflower of the embodiment of the present invention.

Each label lists as follows in figure：

1- plasma generators；2- culture dishes；3- stepper motors.

Embodiment

With reference to embodiment, the present invention is expanded on further.

From atmospheric pressure at room plasma mutation breeding instrument (model as shown in Figure 1：ARTP—IIS；Producer：Without tin source clear sky wood bio tech ltd), concrete operation method is as shown in Figure 2 referring to CN203700376U, its processing system.Start plasma mutation breeding instrument, plasma generator 1 will eject uniform plasma jet downwards, and also controllable stepper motor 3 drives culture dish 2 to rotate in the horizontal direction, makes plasma irradiating more uniform.

Parameter setting：Power is 120W, and plasma exit is 4mm apart from the distance of objective table, and plasma flow amount is 10L/min.

It is as follows using corona treatment sunflower young shoot, step from sunflower young shoot as mutant materials：

(1) day certain herbaceous plants with big flowers young shoot 15 is taken, is scattered in culture dish, culture dish is positioned over to the underface of plasma jet；

(2) start plasma mutation breeding instrument, discharge plasma jet, processing time 8min, 0min control group is set；

(3) sunflower young shoot is taken out, is planted in ventilative good flowerpot, thickness of soil 10cm, flowerpot is positioned at outdoor solar light abundance.

Fig. 3 is the upgrowth situation figure of the sunflower of culture 2 months, and Fig. 3 a are the upgrowth situation figure of 0min control groups and 8min treatment groups October 10, and 0min control groups have bursted forth flower, and 8min control groups only have petal；Fig. 3 b are the upgrowth situation figure on October 27, and the flower of 0min control groups is withered, and treatment group is just bursted forth flower successively, i.e. the florescence substantially postpones.And the root system by observing two groups of sunflowers is found, the root system of 8min treatment groups is significantly shorter than control group.

The above results show that plasma can be used for processing sunflower young shoot, and cause the root length of Sunflower Seedlings to change, so as to influence the florescence.

Plasma is irradiated to vegetable material, can effectively cause the character of plant to change, and helps to expand application of the atmospheric pressure at room plasma in plant treatment field.

Claims

A kind of 1. method using corona treatment sunflower, it is characterised in that comprise the following steps：

(1) make sunflower sample dispersion that culture dish be positioned over to the underface of plasma jet in culture dish；

(2) the sunflower sample in plasma irradiating culture dish is utilized, the time of the plasma irradiating is 10s~60min；

(3) sunflower sample is taken out to be cultivated.
2. the method as described in claim 1, it is characterised in that the sunflower sample includes sunflower seeds, sunflower young shoot And Sunflower Seedlings.
3. method as claimed in claim 2, it is characterised in that when the sunflower sample is sunflower young shoot or Sunflower Seedlings, Sterilized water is added in the culture dish.
4. the method as described in claim 1, it is characterised in that the plasma is atmospheric pressure at room plasma, by exposed gold Category electrode discharges generation under normal pressure, room temperature condition.
5. method as claimed in claim 4, it is characterised in that the atmospheric pressure at room plasma with helium, argon gas, nitrogen, Oxygen, air or its mixed gas produce for source of the gas.
6. method as claimed in claim 5, it is characterised in that the atmospheric pressure at room plasma produces by source of the gas of helium.
7. method as claimed in claim 6, it is characterised in that the throughput of the atmospheric pressure at room plasma is 2~20L/min.
8. method as claimed in claim 7, it is characterised in that the throughput of the atmospheric pressure at room plasma is 10~15L/min.
9. the method as described in any one of claim 1~8, it is characterised in that the time of plasma irradiating described in step (2) For 20s~30min.