CN107429195A - Flavor compositions - Google Patents

Abstract

The invention discloses a kind of flavor compositions, the flavor compositions include the component with collaboration odor property, and the flavor compositions are prepared by following steps：Select the first inactive ingredients in the first mixture, the odor property of first inactive ingredients and candidate active component is most like；Select the second inactive ingredients in first mixture, second inactive ingredients are least similar to the odor property of the candidate active component；Prepare the second mixture, wherein first inactive ingredients are replaced by the candidate active component of equivalent strength, and second inactive ingredients by from the replacement of the known activity materials of the second inactive ingredients identical oder levels；Then if the smell of second mixture is more significant than the smell of first mixture stronger, then select the candidate active component to be used to prepare the composition, think that the candidate active component shows yielding movable in this case.

Description

Flavor compositions

Technical field

The present invention relates to organoleptic flavor compositions with enhancing, the compositions of such flavor compositions is included, And the method for preparing and using such composition.The present invention is including the use of can cooperate with spices caused by the material of blending.

Background technology

Odor detection is realized by the olfactory receptor in the neuron of the olfactory epithelium of nasal cavity.From these nerves The signal of member is delivered to smelling corpusculum and being delivered on the high-rise center of brain to carry out further interpretation in olfactory bulb.Each by Somatic nerves member expresses a kind of olfactory receptor, and the Olfactory receptor neurons of this single type are distributed on whole olfactory epithelium.Come Assembled together on corpusculum from single smell of the output fiber of these sporadoneures in olfactory bulb.Therefore, it is similar to carry out own coding The signal of Olfactory Receptor Neurons of part of the identical odiferous information content of molecular characterization/carrying will tend to be focused in olfactory bulb It is same to smell on corpusculum.Single odourant molecules will generally excite the Olfactory Receptor Neurons of more than one classification, and excitation mode will It is reproducible and peculiar for the molecule.

In the process, first by odorant receptor fragmentation and the feature of odourant molecules is detected.Then, different odor The similar characteristics of molecule strengthen each other at different odorant receptors and in olfactory bulb level.Then by integrally reintegrating to carry For odor perception, this can be simple as single perceive.In this way, from one spend in come out it is many odorous Molecule can excite multiple neurons, and the signal restructuring of the neuron produces single olfactory sensations, and observer can be by the smell Experience is identified as specific to specific flower.Different flowers can send many identical materials, but horizontal and composition difference It is different by reintegrating to produce different sensory perceptions, it can be identified as from different flowers.

This combined method is previously proposed, but involved detailed process is much unknown.The complexity of composition mechanism Property be smell research the feature repeated.Sensory phenomena when the early stage research of smell mixture attempts to mix smell Mapped and classified, and develop term to describe the observed change of observed overall strength.Due to involved Phenomenon complexity, these researchs are only limitted to binary mixture.

Progress on biology level has been demonstrated same intractable.It has been observed that single Olfactory Receptor Neurons are simultaneously whole Close some chemical signals.However, researchers emphasize, the interaction of complexity, and Olfactory Receptor Neurons occur between component Response can not simply speculate to obtain by the response of their component.They are had found, generation is in certainly in receptor neurons, Do not promote the event of the subsequent event at olfactory bulb can be related to perceiving the change of smell, such as due to a kind of odorant agent predominantly Position or the effect for even masking another odorant agent.Natural smell will induce more chemistry to integrate at Olfactory receptor neurons, It can be equivalent to the skew of their smell encoding characteristics so that they can integrally play main function in perception.

Therefore, the problem of challenge institute of researcher's trial understanding smell is potential becomes more fully apparent, and observed The complexity of phenomenon and it is non-linear so that even be difficult to reliably classify.

In nature, cause that scent experience is common and this mixture is normal by the complex mixture of scent molecule It is perceived as single perception.Such case can be observed in the animal and insect that wherein scent signal can drive critical behavior. For example, moth can identify the flower sent more than 60 kinds of materials, 9 kinds therein are detected by olfactory system.These are shown Show and show as the single perception that can drive colored foraging behavior.By being considered as the different characteristic of molecule stimulant being combined into list One a group perceived (by unknown mechanism) smells corpusculum coding unit tissue coding.

In human research, the concrete outcome of such smell mixing is variable and uncertain, but is frequently observed The response of some broad categories.

Smell mixture and not always is necessarily implied that in the convergent property for the process that the high-rise center of smell processing occurs The simple combination of their component.In other words, the smell mixture of complexity may be generally perceived as single entirety by people, simultaneously The experience can also be resolved into and feel subunit.For example, when stench and fragrance mix, the experience can generally be divided, So that it can determine whether the Relative distribution of every kind of odor types for overall smell.Accordingly, there exist an antinomy：Mixture can Single perception experience is perceived as, and the experience can introspection subdivision.

Idiotropic result may not reflect the relative intensity that component stimulates, or even their odor characteristics.However, the mistake Journey can be completely reproducible, and it can be used for design delivering to use the new product of benefit, such as deodorization spices.

Scene is covered such, it can be common that a kind of smell is used to reducing the perception of the second less desirably smell.This is Developed optimizing the common practice of the process and approach.Under the example of cooperative interaction between smell compares It is extremely rare.

In being studied based on the compiling of the result from 520 kinds of binary mixtures, smell mixes under the level higher than threshold value The most probable result closed is that the overall strength of mixture is less than the summation of component intensity, and less than according to Stevens law (Stevens ' Law) is by automatic addition by expected intensity.The intensity of homogenous material is intended as the logarithmic function of its concentration And increase (Stevens law), therefore first in these discoveries be not unexpected, but Article 2 finds more to make People is surprised.It has also been found that a kind of intensity of reduction another kind component in two kinds of components, more than opposite situation.They also found, Adding the three, the 4th or the 5th equality strength component does not cause any increase of bulk strength.It is strong this indicates in action Compression mechanism.

It is as noted above, it is found that synergistic enhancing effect is more rare.When discovered, they are considered as with " synthesizing existing As " associated, wherein when two kinds of components are mixed, produce new different odor qualities.When by the horizontal smell of subthreshold value When agent mixes, some smells are perceived, but can not reasonably illustrate the observation.It follows that these effects Any research will need both measurement intensity and odor characteristics simultaneously.

Concertedness is had described as than higher levels of organoleptic effects expected from the influence general based on unmixed component.One Individual example is to add a kind of odorant agent of subthreshold value amount, so as to cause the perceptive intensity or superthreshold sugarcane of another (beverage) smell The small but measurable increase of the perception sugariness of sugar.It is fragrant or fragrance to think that a kind of a small amount of material of addition can cause once in a while Intensity dramatically increases.However, these examples may be not considered as the certain example of concertedness, unless subthreshold value stimulant itself There is no smell.Due to threshold measurement statistics property (for example, 50% subject can detect its exist and therefore 50% by Examination person is undetectable, and it is existing horizontal), the material added is for many subjects by for superthreshold.

In view of problems, first of concertedness of odor detection in the mankind is shown clearly unambiguously Explanation.Material is the maple lactone mixed with volatile carboxylic acid, acetic acid and butyric acid.In general, in the detection of binary mixture Under threshold value, the threshold concentration of each component tends to the threshold value of the component less than independent smelling, and this phenomenon is referred to as exciting work With (Agonism).

Their research is extended to 3 component mixtures by researchers, but the theme of generality does not occur.They push away Disconnected, the rule of mixture interaction, which is so that, rule of thumb to be handled individually and to every kind of mixture.

In another superthreshold research, examine and mixed using the fruity and the binary of banksia rose smell that are stimulated before nose and after nose Compound.According to the level of banksia rose component, fruity intensity can increased or decrease in the mixture.Reported and cooperateed with based on eeg measurement Property, wherein finding the N1 peak amplitudes of amplification in some mixtures.Other mixtures that smelling arrives after nose scan in electroencephalogram Period shows increased P2 amplitudes.These results can be sense organ and cognitive process during odor perception while work Evidence.

The research of alkyl sulfur compounds and mercaptan is concluded that, i.e. such material with similar chemical constitution Mixing can be characterized by the average effect on all components.

The binary mixture of L- carvols (Carum carvi smell) and eugenol (cloves smell) is at a nostril as one Kind of physical mixture is present, relative to each odorant agent at each nostril individualism (two smell mixing).Record psychophysics Responded with electroencephalogram.Two smell mixture be perceived as it is stronger than physical mixture.The odor characteristics perceived are in two kinds of assessments It is between method and different.The two electroencephalogram responses for smelling mixture show the difference between P1 and N1 (more sense organs) peak value It is different.All results are combined display, and the high-rise center of brain (or after at least smelling corpusculum), there occurs significant L-R hemisphere Reciprocation, and periphery is also the generation place of significant interaction.

In one is announced recently, show that mixture quality (feature) is unrelated with any specific one-component, show me Smell mixture is synthetically more or less perceived as single perception.In its research, the smell and its pleasure of mixture Degree is generally between each in single component.

The WO2002049600 for being incorporated by this paper by reference is disclosed with specific components to promote what is loosened The flavor compositions of emotional state.

The present invention attempts at least some in solving the above problems.Specifically, can be used for producing concertedness to identify The group of the odour component of smell or flavor compositions and flavor compositions therefrom.

The content of the invention

The present invention relates to use that spices caused by the material of blending can be cooperateed with smell or flavor compound.The present invention Also include the product formed by mixing such spices.

In one aspect of the invention, a kind of method by preparing flavor compositions including material may be present, it is described Material when replace in any one in multicomponent embodiment as described herein when there is the component of similar odor characteristics, relative to The similar of disclosed non-elastic component uses the increase that intensity is provided for these new blends.

Brief description of the drawings

Fig. 1 is to show that threshold value is approximately schemed.

Fig. 2 is the bar chart for the standardized intensity fraction for showing embodiment 1-12.

Fig. 3 is the bar chart for the mean intensity fraction for showing embodiment A-F.

Fig. 4 is the bar chart for the mean intensity fraction for showing embodiment G-O.

Fig. 5 is the block diagram for showing odd number smell group.

Fig. 6 is the block diagram for showing even number smell group.

Fig. 7 is the figure for the mean sampled intensity for showing spices.

Embodiment

The present invention surprisingly it has been found that, the particular combination of composition can be used to produce cooperative effect, wherein composition in mixture Organoleptic effects or as an entirety mixture organoleptic effects be more than the influence based on unmixed component desired by. In addition, realize the phase in user such as people the present invention relates to the composition including cooperative effect, and using such composition The method for hoping response.

Those compositions more prominent than expection are referred to herein as " elasticity " material in the mixture, and are not bound by the limit of opinion System, it has been found that some components of flavor compositions are more more elastic than other components.The present invention identifies these elastic odour-producing components, Include how to identify such elastic odour-producing component and threshold value is horizontal, and further summarize can be how by they and other perfume (or spice) Material component is advantageously combined.Elastomeric material also can be by their smell with existing other into subassembly, to produce in the mixture Raw new and different odor characteristics.

In the first aspect of the present invention, flavor compositions include the component from specific group.Described below group is referred to as group 1A, group 1B and group 1C.The flavor compositions of the present invention can include from a group 1A, group 1B and organize one, two or all in 1C One or more components of three.

First component (group 1A) is selected from the group being made up of following item：Acetyl-.alpha.-cedrene, synthetic camphor powder, cedar oil, eucalyptus Set brain, cinnamic acid (10), cistus creticus labdanum, citral dimethyl acetal, sounds of nature Moschus, ligustral, β-damascone (10), δ-damascone (10), Ebanol (10), Ethyl vanillin (10), eugenol, Galbanone (10), γ-ten one Alkanoic acid lactone, piperonal, jasminolene, ambrotone, α-daphnone, convallarol in May, estragole, Chinese cassia tree It is sour methyl esters, the methyl butyrate of ethyl 2, Silvanone, Silvial, alpha-terpineol, caproic acid allyl ester, thunder ice promise star (10), big Anisaldehyde (10), black pepper oil, polysantalol (10), Habanolide, dihydroeugenol, melonal, Violetyne (10), Methyl benzoate, raspberry ketone and their mixture.Group 1A includes activity or bullet in the flavor compositions for the present invention The component of property component.

In this specification in the whole text, when each component includes " (10) ", it represents described material in a solvent 10% molten Liquid, it is therefore preferable to tasteless solvent, including such as dipropylene glycol.

Second component (group 1B) is selected from the group being made up of following item：Alkylol, phenylalkyl alcohol, terpene hydrocarbon or theirs is mixed Compound.Group 1B component can be as the part addition of natural oils.Group 1B component is being described herein as " accelerator ".

The specific example of group 1B components includes：Linalool, orange terpenes, phenyl propanol, phenylethanol, alpha-terpineol, May Convallarol, rose alcohol, citronellol, tetrahydrogeraniol, tetrahydrolinalool, geraniol and their mixture.Have found group 1B component further enhances the cooperative effect of group 1A component.

Third component (group 1C) may be selected from the group being made up of following item：Aldehyde C12 (10), anethole, Ambermax (10), second Sour isobornyl thiocyanoacetate, watermelon ketone 1951 (10), cumarin, cumal (10), oil of ginger, synthesis oak moss, patchouli oil, methyl decene Alcohol, vetiver oil；And their mixture.Material from group 1C also can be as the part addition of natural oils.Come from Group 1C material is optional in the composition.

As noted, one or more components in one, two or three group can be used for the present invention.From group 1A one or more components are present in composition with following amount：By weight of the composition about 20% to about 80%, Or by weight of the composition about 30% to about 80% or by weight of the composition about 40% to about 80% or By weight of the composition about 50% to about 80% or by weight of the composition about 30% to about 60% or about 50% to about 60%.The quantity of each component from group 1A can be one, two, three, four or more than four kinds.When it is present, come from Group 1B one or more components are present in composition with following amount：By weight of the composition about 5% to about 50%, By weight of the composition about 15% to about 50% or described composition about 25% to about 50% or by described group The weight meter about 15% to about 25% or about 10% to about 20% of compound.When including in the composition when, from each of group 1B The quantity of component can be one, two, three, four or more than four kinds.When it is present, the component from group 1C is present in combination with following amount In thing：At most about the 35% of the composition or by weight of the composition about 18% or less.When being included in composition When middle, the quantity of each component from group 1C can be one, two, three, four or more than four kinds.

Therefore, one aspect of the present invention includes foregoing group of 1A, group 1B and the combination for organizing 1C.

The second aspect of the present invention includes the material that their uses in the composition are restricted, or is left out Material.Two groups of these materials in the present invention be present.Group 2A and group 2B.

Group 2A includes allyl-cyclohexyl base ester, Asiatic sweet leaf alcohol (Bangalol), bougeonal (Bourgeonal), currant alkali, glycidyl methyl phenyl ethyl ester, vinyl tridecanoic acid ester, lily of the valley pyrans, lavender Oxane (Herboxane), cis 3 carbonic acid hexenyl methyl ester, Jasmatone, lemonile, lilial, artificial neroli oil, methyl Lactone (Methyl Laitone), phenylacetic acid phenyl chlorocarbonate, rose oxide (Rose oxide), methyl phenyl carbinyl acetate, spy Lars Moschus, vanillyl alcohol, Java Cananga Oil (Ylang oil) and their mixture.

Group 2B includes isononyl acetate, bergamio and their mixture.

When it is present, organize 2A or organize the material in 2B to be no more than about 1.0% by weight of the composition, and more 0.6% is preferably no more than about by weight of the composition to have an independent existence in combination (except the component as natural oil) In thing.Therefore, 2A material is organized, when independently of being present in natural oil in use, can be by the weight of the flavor compositions The amount of meter 0% to about 1.0% or at most about 0.6% is present.Similarly, 2B material is organized, when independently of being present in natural oil In use, can based on the weight of the flavor compositions 0% to about 1.0% or at most about 0.6% amount exist.

What the total concentration of the non-essential oil addition of the material from group 2A and group 2B accounted for total flavor compositions is less than 2 weight %, And more advantageously account for the less than about 1 weight % of total flavor compositions.In some embodiments, flavor compositions of the invention Without any material from group 2A, and in some embodiments, flavor compositions of the invention are free of from group 2B's Any material.

All percentages are based on the gross weight of material in flavor compositions (except the part addition as natural essential oil Material), the percent of total of essential oil or the like (wherein its be specify composition), and be the 10 of the actual concentrations of pure material Times, wherein it is marked as being followed by (10), such as aldehyde C12 (10).The situation of two or more groups is appeared in material In, its distribution should be regarded as distributing between the groups (for example, convallarol in May, alpha-terpineol)；Such as 50 between two groups:50.

The present invention surprisingly it has been found that, the particular combination of composition can be used to produce collaboration smell or flavor compositions.It is not bound by The limitation of opinion, it has been found that some components of flavor compositions are more more elastic than other components.Elastic odour-producing component is for whole group Compound is provided than the odour-producing component for the bigger feature for being provided expected script based on the odor property of homogenous material.This hair Bright to identify elastic odour-producing component, the component is easier identification in the mixture and their odor characteristics become as one The clear component of the odor characteristics of individual overall mixture.Another benefit of the present invention is that the presence of elastomeric material can cause Caused new and different odor characteristics in mixture.The present invention is quite useful because its realize provide it is stronger, Or more complicated or unique spices, while avoid adding more multicomponent needs in the composition.For example, when in combinations of perfumes In the case of using less elastic component in thing, elastic component can assign higher perceptive intensity.

When producing smell mixture by the equality strength composition of equal proportion, include significant percentage of " elastomeric material " Mixture it is generally associated with the perceptive intensity higher than the mixture that wherein they are not present.

The odor characteristics contribution of second group of material " non-elastic material " reduces when being mixed with more elastomeric materials.Some In composition, these non-elastic materials can be covered completely.Therefore, in composition non-elastic material such as organize 2A and group 2B in arrange The amount of those gone out should be limited to above-mentioned level, if you are using.Elastic component, those in 1A are such as organized, should be with than a group 2A And/or the significantly high amount of component in group 2B is present.

Therefore, foregoing aspect of the invention includes flavor compositions, and the flavor compositions include and from group 2A and organize 2B One or more of component combination at least one one or more components in group 1A, 1B and 1C.

When elastomeric material and/or the mixture comprising the elastomeric material strengthen, the 3rd group of material tends to exist, but It is the smell contribution that their own does not show this protrusion generally.These are a group 1B accelerator.Many in group 1B accelerator It is alcohol, it is conventional intermingling material.The present invention surprisingly it has been found that, group 1B materials promote the tribute of flavor compositions elastomeric material Offer.The intensity of group 1B accelerator increase elastic components.The intensity of increase group 1A materials is organized 1B accelerator by group 1B accelerator Smell insignificantly comes out.Group 1B accelerator is optionally comprised in the spices of the present invention.

The threshold concentration of odour-producing component is the perceived Cmin of smell.These behaviors can be shown in the mixture Come, wherein all components exist under threshold concentration using equal parts as equality strength stimulant.Threshold concentration, which can be considered, to be used for The standard level of equality strength concentration is produced, the threshold concentration can be relatively clearly identified for all material.If mixed Do not interacted between the equality strength component of compound, then every kind of material will be perceived comparably.If some materials Material becomes more prominent in smell and/or strong, then is judged as enhancing theirs by the presence of other materials Smell.Therefore, mixture is formed by the use of equality strength material to provide to identify in mixture or for being used as an entirety Mixture when and how the methods availalbe that can strengthen.In the threshold level of the perception of odour-producing component, such enhancing is more Easily identification.

The available solvent of liquid phase sample for preparing threshold concentration is dipropylene glycol (dpg).The concentration of spices material exists Usual very little in such composition so that the physical effect between material will be very small under the threshold value, and main effects will be Aesthetic.

The present invention includes flavor compositions, and the flavor compositions include the quilt under the intensity higher than threshold value in the mixture All the time perceive and their concentration is maintained at the component of threshold concentration level.Therefore, by the way that invention increases a kind of or more The intensity of the smell of kind component, even if the actual amount of one or more components is in threshold concentration level.

Pay attention to, the specific aromatic of odor characteristics can be increased by using a small amount of additions (trivial addition) Intensity, but the present invention has surmounted using only a small amount of additions as described herein.A small amount of additions, which include addition, has identical smell fragrant The material of tune is to realize bigger smell.For example, can be in threshold concentration or less than combined material under threshold concentration so that in group They produce the smell higher than threshold value perception level during conjunction.This each can partly or entirely act on same receptor by only combining Material realize.Such material group will be typically appraisable, because they have similar smell or shared smell fragrant Adjust.For example, the different rose taste combinations of materials of subthreshold value amount can be produced into the superthreshold mixture with rose scent.However, Only this is not mechanism of the invention.Elastic odour-producing component in the composition of the present invention produces the effect and odour intensity of enhancing Benefit.This can when different in the presence of the other materials with shared odor characteristics in the case of realize.Certainly, the present invention is not excluded for They are used in combination with such material.Describe material of the only blending with similar odor characteristics by way of example above Method, the alternative and " being remarkably reinforced " based on a small amount of additive effects are distinguished.

In addition to the elastic odour-producing component that the present invention uses, the second component can be added.The second component material added This protrusion smell effect itself can not be played in the overall odor characteristics of mixture.When among most strong component, it May not be perceived, however, they will not strong dilution, will not also detract the intensive properties of the mixture comprising elastomeric material Energy.It has surprisingly been found that the combination of elastic odour-producing component and the second component produces the mixture with available enhancing performance (for example, higher perceptive intensity of the mixture of flexible odour-producing component).

It can be used according to the spices of the present invention or perfume composition in multiple product.As used herein, term " production Product " should refer to the product for including flavor compositions described above, and including consumer products, curable product etc..Such product can Take various forms, including powder, strip, bar-shaped, tablet, creams, mousse, gel, lotion, liquid, spray and sheet material.It is such In product the amount of flavor compositions can 0.05% based on its weight (as example in the small skin creams of smell) to 30% (such as Such as in fine fragrance) in the range of.It is known, and existing for flavor compositions being mixed in the product of these types Technology can be used for spices of the incorporation for the present invention.Various methods in flavor compositions to be mixed to product are included spices Composition is mixed directly into product or on product, but another possibility is to adsorb flavor compositions in carrier material On, then spices-plus-carrier mixture are blended into product.

In order to provide conciser description, some quantitative expressions given herein are not limited with term " about ".Should Understand, regardless of whether clearly having used term " about ", each amount given in this article, which is intended to, refers to actual set-point, and And be also intended to refer to by one of ordinary skill in the art can the approximation of these set-points that goes out of reasonable expectation, including these give Definite value as the approximation caused by experiment and/or measuring condition.

The present invention includes flavor compositions and the product containing such flavor compositions, and uses such flavor compositions With the method for product.Application method includes providing flavor compositions or product as described herein to people and made one obtained by smelling Smell is to realize desired effects.Desired effects may include for example, to user (such as people) provide emotional benefits, cognition benefit, And/or the improved interaction with other perceptual models.

Present invention also offers a kind of perfume (or spice) evaluated some spices/smells and determine the benefit available for the identification present invention The method of the threshold concentration of material or aromatic.The evaluation and then the perfume (or spice) available for expectation aromatic of the generation with expectation threshold quantity Feed composition (or product containing flavor compositions).It thus provides it is a kind of determine aromatic threshold quantity and use comment The method that valency result prepares flavor compositions.This method may also include to form the product with flavor compositions.

In following examples and description, this method includes the use of solvent.Solvent in embodiment is dipropylene glycol, is had When be referred to herein as dpg, but can also be used other smells small or scentless solvent.

In these embodiments, it is first determined threshold value of every kind of composition in dpg, then it is described it is horizontal under by it is every kind of into Divide in incorporation spices.Also utilize and produce spices with about 0.3 times of all the components existing for threshold value, and another group of utilization is with 0.1 All the components produce times existing for threshold concentration.For illustrative purposes, experiment is existed using the 10ml aliquots of spices below Carried out in 125ml brown glass wide-mouth bottles.

Threshold measurement

For finding out the detection of every kind of odour component from liquid solution and/or a kind of suitable method of recognition threshold Derived from the method for limits (Method of Limits) (be described in ASTM'Manual on Sensory Testing Methods', In STP 434 (1968), American Soc for Testing Materials, Philadelphia, Pa.19103, USA, Entire contents are herein incorporated by reference).Initial experiment is carried out to determine approximate threshold level.Prepare the concentration system of sample Arrange and dilute, until fragrance scent can not be differentiated.Then, by with start less than threshold level ascending concentration series double Fragrance component in propane diols is presented to every evaluator, and evaluator then judges to specify the presence of odor qualities in each sample Or it is not present.Continue the series, until judging to change (from " being not present " to " presence ").By the data from more than 15 assessments Merge, and analyze, within release the targeted odorous in 50% assessment and will be detected in the series of (and/or recognizing) Concentration.

Assuming that recall rate and log₁₀Relation between concentration is S-shaped, therefore for the recall rate of every kind of ingredient prediction 50%, Follow following function and obtain fit line：

Wherein y is recall rate percentage, and x is the log of percentage of the composition in dipropylene glycol₁₀, k is determination S types The constant of the gradient of function, and threshold value is concentration value at the flex point of sigmoid curve (and therefore, under 50% recall rate Concentration).

Approximation is taken to k and threshold value value, then using Microsoft XL 2007 solver add-in module fits, So that the root-mean-square error (RMSE) between point of observation and future position is minimum.The gained RMSE of all fit lines less than 10% and It is considered as acceptable.Fig. 1 shows that a kind of threshold value of sample fragrance component is approximate.

The assessment of odour intensity measurement

Masculinity and femininity evaluator team is used in the evaluation of sample strength.In this work, evaluator is between 25 Year and between 65 years old.The ability for being correctly ordered the odour intensity of the serial dilutions (in dpg) of fragrance component based on them To select them to be evaluated.It is the serial dilutions listed in the following table that smell, which assesses the standard flavorant composition used in meeting, The benzyl acetate of middle preparation.Each dilution is associated with odour intensity fraction.Other materials can be used in a similar manner.

As above standard dilution is provided and is provided for during evaluation to help evaluator's during evaluation With reference to.

The embodiment tested is prepared as described herein.Embodiment is by or greater than their own threshold concentration Material mixture in dpg dilution composition.In general, the every kind of solution of about 10g is placed on to the 125ml of capping In wide-mouth bottle and it is set to balance at room temperature minimum 2 hours.Assessed by evaluator's removal lid and smelling inclusion. Wide-mouth bottle is assessed with random sequence.The fraction that evaluator is distributed between 0 and 8 to each sample, wherein 0 corresponds to no gas Taste, 8 represent smell strongly.Hereafter, at least 15 times assessments are obtained for each sample.

, can be by will be each in the case of the assessment of sample is carried out through meeting several times and/or with different subjects The result of sample is normalized to be advantageous to the comparison between sample between meeting and evaluator.This can for example can be obtained in evaluator When must excessively have the sample for staying in a meeting and reliably assessing.The data of embodiment 1 to 12 are divided in this way Analysis, as described below.

The sample of a section is presented to evaluator in a series of meetings, to reduce the assessment related to a large amount of samples It is tired and inconsistent.As follows by the score normalization of each evaluator：For each evaluator, calculate all independent in the meeting The average value of fractionAnd calculate the sample standard deviation (s of identical fraction set_{(evaluator, meeting)}).Use These statistical values, each in the data point of evaluator is converted into standardized score, will be i-th point of each evaluator Number (x_i) it is recalculated as (x_std,i), it is as follows：

Data are further analyzed using variance analysis.Then all of all evaluators are calculated for each sample The average value of standardized score

Embodiment is prepared using a variety of fragrance ingredients listed in Table A.It is a small amount of every kind of according to known to the addition into bottle Stock solution (in dpg) and prepare all examples with being diluted to the desired amount of principle constant volume using other clean dpg Property mixture.Preferable stock solution causes 20 μ L every kind of composition stock solution, when further dilute in 20mL solution is amounted to The solution of all the components of the estimation threshold concentration in every kind of composition will be delivered when releasing.

Stock solution is prepared by gravimetric analysis in step is serially diluted：For example, in order to prepare a kind of composition 0.0005% solution, 0.50g is added in 9.50g dpg, obtains amounting to 10.00g 5% solution；Then by 0.15g this Solution is diluted in 14.85g dpg, obtains amounting to 15g 0.05% solution；Then by the way that 0.15g 0.05% solution is added It is added in 14.85g dpg to dilute this second solution with identical dilution gfactor, obtains 15g 0.0005% solution.

Mixture stoste is stored in refrigerator, is stored in the appearance that there is very small remaining headroom in superjacent (make volatilisation loss minimum) in device.

20.0g is amounted to prepare each implementation by the way that every kind of stock solution of aim parameter to be added in bottle and complement to Example.Then stir each mixture and make its balance.Each mixture uses as it is, and enters one with the factor of 3/10 and 1/10 Step dilution, to produce subthreshold value mixture.In this way, each mixture of 3 concentration is prepared：(1) every kind of component is in threshold value Concentration, (2) every kind of component are in 0.3* threshold concentrations, and (3) every kind of component is in 0.1* threshold concentrations.

Table A

Embodiment 1：By the dpg solution of the 0.10% of 141.5 μ L cis -3- hexenols, 50.7 μ L 5.00% snow The dpg solution of pine tar, 6.1 μ L 9.93% methyl pellet put forward the dpg solution of man of great strength, 44.6 μ L 1.00% crocetin second The dpg solution of the dpg solution of ester and 18.4 μ L 3.34% citronellol is added in 19.74mL dpg and mixed.

Embodiment 2：By the dpg solution of 18.4 μ L 3.50% linalool, the dpg of 15.1 μ L 0.98% Ebanol Solution, the 18.9 μ L dpg solution of 7.32% methyl cinnamate, 18.9 μ L 7.01% benzyl acetate dpg solution, with And 18.4 the μ L dpg solution of 3.34% citronellol be added in 19.91mL dpg and mix.

Embodiment 3：By the dpg solution of 189.3 μ L 3.25% citral dimethyl acetal, 8.9 μ L 5.00% The dpg solution of 1.50% mace oil and 6.9 μ L 0.01% matricariaester of the dpg solution of estragole, 20 μ L Dpg solution be added in 19.77mL dpg and mix.

Embodiment 4：By 1.15% dihydro bay of the dpg solution of 195.5 μ L 2.10% alpha-terpineol, 18.2 μ L The dpg solution of enol, 19.5 μ L 1.00% eugenol dpg solution, 6.9 μ L 0.05% methyl -2- ethyl butyrates Dpg solution and the 88.7 μ L dpg solution of 0.50% phenylethyl alcohol be added in 19.67mL dpg and mix.

Embodiment 5：By the dpg solution of 18.4 μ L 3.50% linalool, the dpg of 8.9 μ L 0.04% cineole Solution, 9.9 μ L 5.21% cashmeran dpg solution and 9.2 μ L 0.55% δ-damascone dpg it is molten Liquid is added in 19.95mL dpg and mixed.

Embodiment 6：By the dpg solution of 5 μ L 1.01% ligustral, 15.1 μ L 4.99% cistus creticus ladanum oil Dpg solution, the 13.8 μ L dpg solution of 10.00% methyl cinnamate, 6.9 μ L 0.01% matricariaester dpg it is molten The dpg solution of liquid and 126.2 μ L 0.05% geranium oil is added in 19.83mL dpg and mixed.

Embodiment 7：By the dpg solution of 10 μ L 0.02% p-methylphenyl methyl ether, 19.2 μ L 13.11% acetic acid The dpg solution of different nonyl ester, 20 μ L 0.0010% methyl lactone dpg solution, 18.2 μ L 1.20% ethyl-methyl benzene The dpg solution of the dpg solution of base glycidic ester and 66.3 μ L 0.05% indoles is added in 19.87mL dpg And mix.

Embodiment 8：By the dpg solution of 17 μ L 0.12% cyclamen aldehyde, 19.2 μ L 13.11% isononyl acetate Dpg solution, the 18.2 μ L dpg solution of 0.42% cumarin, 18.3 μ L 9.49% allyl-cyclohexyl base ester Dpg solution and the 103 μ L dpg solution of 1.00% rose alcohol be added in 19.82mL dpg and mix.

Embodiment 9：By the dpg solution of 17.8 μ L 0.00012% lily of the valley pyrans, 141.5 μ L 0.00071% it is suitable The dpg solution and 186.9 μ L of the dpg solution of formula -3- carbonic acid hexenyl methyl esters, 19.4 μ L 0.00053% patchouli oil The dpg solution of 0.0075% phenylacetic acid phenethyl ester be added in 19.63mL dpg and mix.

Embodiment 10：By 2.48% vetiver of 17.1 μ L 1.02% Galbanone dpg solution, 17.1 μ L 1.21% artificial neroli oil of the dpg solution of oil, the dpg solution of 19.5 μ L 1.00% eugenol and 17.7 μ L Dpg solution is added in 19.93mL dpg and mixed.

Embodiment 11：By 0.013% second of the dpg solution of 183.3 μ L 0.011% bergamio, 19.2 μ L The dpg solution of the different nonyl ester of acid, the dpg solution of 18.5 μ L 0.0025% Ethyl vanillin, 18.3 μ L 0.0087% third The dpg solution of the dpg solution of sour allyl cyclohexyl acetate and 126.2 μ L 0.00032% geranium oil is added to In 19.63mL dpg and mix.

Embodiment 12：By the dpg solution of 17.8 μ L 0.14% lily of the valley pyrans, 22 μ L 5.00% isobomyl The dpg solution of ester, the 18.5 μ L dpg solution of 2.68% Ethyl vanillin, 29.7 μ L 5.04% phenylacetic acid benzene second The dpg solution of base ester is added in 19.91mL dpg and mixed.

The scope of smell according to obtained by the present invention is extremely wide, and is not limited to any particular section.Perfume (or spice) in table 3 below The smell description of feed composition shows the non-limiting example of the width of odor types according to obtained by the present invention.Intensity results Shown in table 4.

Table 3

Table 4

Dual factors variance (two-way ANOVA) analysis is carried out to data group：Two selected qualitative forecasting factors are claimed For " embodiment ", corresponding to the sample assessed, and " concentration ", corresponding to three sample strengths；Threshold value, 0.3 × threshold value and 0.1 × threshold value.

ANOVA determine dual relief valve be significantly fitted for the data of 95% confidence level (F=23.440, d.f.=13, p<0.05,R²=0.706).The analysis of I types quadratic sum shows embodiment (F=9.703, d.f.=11, p<And concentration (F 0.05) =98.993, d.f.=2, p<0.05) factor is variational to data remarkably promotes effect, because in Near Threshold concentration Sample between show such significant difference.Models fitting statistical value is shown in table 5 and table 6.

Table 5

Table 6

Fig. 2 shows the average value and 95% confidential interval of the standardized score of embodiment；It should be noted that embodiment 1-6 is shown Go out for credible score>0, and embodiment 7-12 has negative average value.

The analysis of Duncan afterwards (Post-hoc Duncan analysis) of sample is shown according to an embodiment of the invention Significant difference between (embodiment 1-6) and comparative example 7-12.In table 7, between the member of the group with same letter not Mean difference be present, and significant difference (critical p=0.05) between the average value of different groups of sample be present.Do not find to belong to simultaneously In group A and group B sample.Therefore, it can be said that embodiment 1-6 is significantly better than comparative example 7-12.

Table 7

Embodiment A to O

In a series of further embodiment A into O, assessed respectively using unidirectional opinion rating in independent experiment by subject (description of opinion rating and application thereof is found in ASTM'Manual on Sensory Testing to the intensity of mixture Methods', STP 434 (1968), referring specifically to the 19-22 pages, American Soc for Testing Materials, Philadelphia, Pa.19103, USA, its full text are hereby incorporated herein by).In these level, " no intensity " is chosen as 0, and other intensity are graded as discussed previously.According to the regulae generales system described by above with respect to embodiment 1 to 12 Standby flavor compositions.The percentage by weight of every kind of composition is shown in table 8-13 in composition.By 10ml every kind of spice solution It is placed in 125ml brown glass wide-mouth bottles and makes its balance.Subject assesses wide-mouth bottle inclusion and the smell to perceiving Intensity is graded.The code is repeated through 3 meetings, until having carried out 15 assessments.

Embodiment A to O shows the benefit of the present invention：Worked as according to the mixture of the present invention will be heard in the presence of threshold concentration Get up stronger than the similar mixtures using the active relatively low or inactive material according to the present invention.It is living in embodiment Property is relatively low or inactive component is marked as " nonactive ".For the present invention a part component be marked as " elasticity or Activity ".In addition, group 1a materials and the combination for organizing 1b materials (or similar alkylol), exist with threshold concentration, can be strong with its Degree delivering stimulus to the sense organ.Embodiment A-O average value or average mark is shown in figs. 3 and 4.Black bar shaped instruction 95% is put Believe section.

Table 8

Table 9

Table 10

Table 11

Table 12

Table 13

Using method of testing described above, spices is compared compared with, and spices shows higher according to caused by the present invention Odour intensity, and in some respects, significantly higher odour intensity.For purposes of illustration, careful spices is answered not include Its key odor feature material shared with the other materials in spices.The gas as two species around threshold value or threshold value The additive effect that (or being left out) is effectively minimized this caused by taste excites identical acceptor and therefore described Above threshold activity level is obtained at acceptor.Therefore, spices of the invention is shown as with higher intensity, its be by into point it Between collaboration influence each other caused by.Conventionally this phenomenon is rare.The present invention allows with reliable repeatable side Formula prepares the spices with internal coordination.The invention provides a kind of method for preparing such spices, in addition, spices is certainly Body covers wide smell scope and provides benefit.Spices is typically one kind in the component costly of consumer products, therefore is appointed What such broadly available intensity increase is all valuable for preparing business.

The quick test of elasticity

In another aspect of this invention, the method whether to be demonstrated flexibility for identifying new material, methods described letter be present Carry out singly and relatively quickly.In other methods, include multiple assessments of many component mixtures in balance test design, However, if a kind of test can be designed, wherein new material can be added in correct mixture, the elasticity that test material be present is special Property becomes obvious high likelihood, then the test is preferable.This is to determine the mesh of the alternative method for rapidly testing of elasticity 's.Following used, this method will be referred to as " quick test ".

The method taken is to form two kinds of mixtures, and wherein all the components are all stiff and deposited with threshold concentration .There is also minimum odor property is overlapping between every kind of composition.Then these compositions can be replaced by test material.Elastomeric material Partly limited by the trend of the intensity of mixture of the increase comprising the elastomeric material.New component can be by measurement when known The perceptive intensity that occurs when being replaced of non-elastic material change to classify.

If replacing inactive ingredients by using new test material, the intensity of mixture dramatically increases, then is introduced into collaboration Interaction, and test material shows " elasticity " activity, term as used herein.

The composition of test mixture

The mixture of inert matter is designed using smell kind same as described above.Fragrance spectra is subdivided into ten wide Smell kind.These classifications are：The fragrance of a flower, aldehyde perfume, citrus perfume/pure and fresh, green grass perfume/water sample, herbal medicine perfume, the banksia rose/ambre, powder Sample/Moschus, Xin Xiang, fruity-light, fruity-strong.These describe language and are usually used in field of perfumery, and are this area skills Known to art personnel.They are assigned to two kinds of mixtures so that a kind of mixture includes following smell group：Aldehyde is fragrant, green grass Perfume (or spice)/water sample, the banksia rose/ambre, Xin Xiang and fruity-strong；And another mixture is comprising citrus is fragrant/pure and fresh, herbal medicine is fragrant, powder Sample/Moschus, fruity-light and fragrance of a flower.New test material should replace one in non-active material in appropriate test mixing thing Kind, preferably replace odor property and the most like inert matter of test material.

It has been found by the present inventors that when two kinds of active materials in mixture be present, quickly test most effective.With in the absence of The mixture of active material is compared, and this method generally realizes the significant increase of intensity.

The general introduction of quick test procedure

Preferable quick test procedure is summarized in hereafter.

A kind of verified test material replaced via active material preferably used in wherein non-active material.Cause This, two kinds of each nominated with the mixture of inert matter of " standard " active material are used together.Standard activity Material is all will to mix the material in test mixing thing with threshold concentration together with test material.Two kinds of substituents should produce tool There is the mixture than the significantly higher intensity of original stock in the absence of active material.Two kinds of " standard " active materials have difference Smell simultaneously belongs to different odor classification.They are listed in experimental section below.

The present invention include be used for identify and select new active substance method, thus the second non-active material by or not by In the case of known active material substitution, when candidate's material material replaces one kind in test materials described two for this purpose In non-active material when, its deliver enhancing intensity (on standard scale as described herein, more than or equal to one unit). Preferable active material and inert matter describe in the description.The present invention include prepare using substitution non-active material or Non-active material prepares flavor compositions.

The first stage of test is which kind of classification characterization test material belongs to and select with non-with its most similar type The mixture of active material.Unknown material will replace the non-elastic material from identical smell group.This mixture will act as into one Walk the basis explored.Then, should select to be judged as most different from unknown material non-elastic materials in terms of smell.Should Non- elastomeric material will be substituted by the elastomeric material from identical smell kind.The example of the elastomeric material of every kind of smell kind exists Provided in above-mentioned text.

In view of the expected result for the effective benefit for determining substitution, and prepare the composition with suitable resilient component most Whole target, the present invention include method for rapidly testing.Methods described can be used for identifying and selecting new active substance, thus when candidate's material Material replace test materials described two for this purpose in it is a kind of in non-active material when, its deliver enhancing intensity (for example, on standard scale as described herein, more than or equal to one unit).This can the second non-active material by or not by Known activity material is carried out in the case of substituting.Described above is preferable active material and inert matter.

Method may include following process.First, user identifies and considers the inactive ingredients in two kinds of test mixing things In each.In step 1, inactive ingredients are selected, it is most like with candidate material in terms of odor characteristics.It is this to be reflected Fixed component is referred to as " most like " inert matter.This, which will be identified, any in two kinds of test mixing things will be used below step In.Next step (step 2) for which kind of non-active material and the candidate material in test mixing thing of the identification selected from step 1 most It is different.The identification of most different non-active materials is optional, it is preferable, however, that identifying the component to maximize difference.Identification " most different " material will by from identical smell kind known activity material replace.

Third step is at least one of two kinds of inert matters identified by substituting in above-mentioned steps 1 and 2, and Advantageously two kinds (most like inert matter and most different inert matters) prepares selected test mixing thing again.Example Such as, most like inert matter (being identified by step 1) can be removed and replaced with the candidate material of equality strength concentration, and can Least similar material is removed, and is replaced with the known activity material of the equality strength concentration from step 2.Described above is work The example of the suitable concentration of property material.The above method can be used to find the threshold concentration of candidate material.

In step 4, the method for optimizing described in hypomere can be used to assess the intensity of the new blend from step 3.Such as The significantly stronger initial testing mixture (for example, intensity is unit or more) in inert matter of fruit new blend, then Think that candidate material has shown elastic activity.The conclusion can be used for the flavor compositions that exploitation includes candidate material.Therefore, The use of the modified flavor compositions of the method exploitation of the present invention is probably useful, thus, at least one component has been substituted, Such as active component substitution inactive ingredients or vice versa are as the same.

The assessment of " elasticity activity "：The intensity for mixing the new blend of new test material and standard activity material should be relative Assessed in the intensity of the relative mixture of five kinds of non-active materials.It is preferred that use the intensity being used in hereafter experimental section Scale.This is sense organ scale, and wherein organoleptic scores are shown by the normal concentration of the benzyl acetate in DPG.If new mixing Thing is significantly stronger than the blend (for example, using the scale, more than one unit) of inert matter, then it is believed that new test material Show " elasticity " activity.Then the composition comprising elastomeric material can be prepared.

The formula of two kinds of test mixing things, and two kinds of standard activity materials being used together with each are in experimental section In provide.

Experimental section 1：The quick test of elastic component

Sample preparation

All samples and reference solution are made up of dilution, in units of dpg.10g every kind of solution is placed in capping In 100ml wide-mouth bottles and it is set to balance at room temperature minimum 2 hours.By removing lid and smelling content, and change lid and come Assessed.

The fragment of sample is presented to evaluator with a series of meetings, to reduce the assessment fatigue associated with a large amount of samples With it is inconsistent.The order that sample is presented is from the most weak intensity of hypothesis to the most intensity of hypothesis, so that the residual of strong sample is most Smallization.Baseline mixture is presented first, hereafter all other test mixing thing randomization.

Evaluation procedure

When evaluating sample strength, the masculinity and femininity evaluator troop between 25 years old and 65 years old is used.Based on it to perfume (or spice) Expect a series of ability of the correct grading of the odour intensity of dilutions (in DPG, dpg) of composition, select them to be commented Valency.

Ionization meter is on the basis of the benzyl acetate of normal concentration.Before meeting is assessed, acetic acid is presented to group member Benzyl ester, benzyl acetate prepare (in terms of dpg) in the form of a series of dilutions, as listed in following table.Every kind of dilution with Odour intensity fraction is associated.

i：The standard dilutions of benzyl acetate in terms of dpg, there is corresponding fraction：

As above standard dilution is provided and is provided for during evaluation to help evaluator's during evaluation With reference to.

Laboratory sample：

Prepare and assess two groups of experimental mixtures：Group 1：1a, 1b, 1c, 1d and 1e；With group 2：2a, 2b, 2c, 2d and 2e.

When developing these samples, a kind of non-active ingredient is used in smell group each is selected：1, aldehyde is fragrant；2, citrus It is fragrant/pure and fresh；3, green grass perfume, water sample；4, herbal medicine is fragrant；5, the banksia rose, ambre；6, powder sample, Moschus；7, Xin Xiang；8, what the smell of fruits is very sweet； 9, fruity is light；10, the fragrance of a flower.Then these materials are used to prepare two kind of 5 component mixture, its baseline sample formed in every group Product.

1 sample of group is only made by odd number smell group；2 samples of group are only made by even number smell group.This precautionary measures ensure All samples are made by the composition selected from non-conterminous smell group, so as to minimize in every kind of mixture between inactive ingredients Odor property it is any overlapping.All the components are mixed (in terms of dpg) using the above method by its threshold concentration estimated.

Each group is made up of 5 samples：

(a) the baseline mixture only non-active ingredient as known to 5 kinds is made, every kind of to be selected from different non-conterminous smell groups.

(b) the manufactured base in the case where a kind of non-active ingredient is replaced from the known activity composition of identical smell group Line mixture pattern, the mixture of 4 kinds of non-active ingredients and a kind of active material is obtained (for example, mixture 1b, which is included, comes from nextpage 7,7act active material is organized in table).

(c) this second, " b " mixture forms the basis of the 3rd mixture (c), wherein the second non-active ingredient is by known Active component substitution, (come so as to produce the mixture of 3 kinds of non-active ingredients and 2 kinds of active components for example, mixture 1c is included From the group 7 and 9,7act and 9act active material in lower page table).

(d) and (e) prepares two kinds of subsequent mixtures (d) and (e), and every kind of mixture includes 3 kinds of non-active ingredients and 2 kinds of work Property composition, it uses mixture " c " to be used as its starting point.In these mixtures, one in the two kinds of active components from " c " Kind is replaced by the alternative known activity composition in identical smell group.

Virtual test material is provided for the new active substance of (d) and (e) mixture to prove the serviceability of method of testing (or deadness).

10 kinds of samples of gained are described in the table immediately below.

ii：The formula of 2 samples of group 1 and group

Organoleptic analysis

The perceptive intensity of above-mentioned all mixtures is assessed with reference to benzyl acetate-fixing intensity scale.Record mean intensity simultaneously Compare, to assess compared with corresponding 5 component mixture of inert matter, comprising test material and known activity material whether Cause dramatically increasing for perceptive intensity.

Data analysis

Mean intensity fraction, n=15：

iii:Average table：1 (odd number smell group) of group

iv：Average table：1 (odd number smell group) of group

Input dependent variable of the intensity fraction of every kind of sample sets as two-way ANOVA (ANOVA).Each analysis has Two factors of identical：1) " observation ", there are 15 grades, is graded corresponding to every group of group member, 2) " sample ", there are 5 grades, correspond to Sample a-c in respective sample mixture group.

Fig. 5 shows the average value figure of the intensity of mixture in group 1.In the figure, with the posts of different alphabetic flags (for example, A, B or AB be to C, but is not A to AB) it is dramatically different.It was found that change (F=13.4, the df of the significant predictive data set of ANOVA models (model)=18, p<0.01).I types SS analyses discloses observation and sample factor it is notable it is main influence, disclose sample and Individual group member's uses difference related between scale but consistent.

v：I types quadratic sum analysis (group 1)

The significant difference that Multiple range test is disclosed under three ranks between sample afterwards (does not share identical group in Distal Right row The significant difference of sample, p<0.05)：

vi:Duncan analyses (group 1) afterwards

Conclusion ANOVA (group 1)：Thunder ice promise star and eugenol be it is active, i.e., it is elastic, in definition illustrated above.

ANOVA, organize 2 (even number sets)：

Fig. 6 shows the average value figure of the intensity of mixture in group 2.In the figure, with the posts of different alphabetic flags (for example, A, B or AB be to C, but is not A to AB) it is dramatically different.It was found that change (F=13.4, the df of the significant predictive data set of ANOVA models (model)=18, p<0.01).I types SS analyses (nextpage) disclose observation and the notable main influence of sample factor, so as to disclose Difference related but consistent between observer and sample.

vii：I types quadratic sum analysis (group 2)

The significant difference under four ranks of Multiple range test (Duncan methods) announcement between sample is (in Distal Right row afterwards The not shared identical group significant difference of sample, p<0.05)：

viii：Duncan analyses (group 2) afterwards

Conclusion (ANOVA groups 2)：Both raspberry ketone and petitgrain bigarade oil are active, i.e. bullet in definition illustrated above Property.

Result based on two kinds of ANOVA, it was demonstrated that following principle：In the case where two kinds of active components be present, gained mixes Compound is considerably stronger than baseline mixture.Prove that two kinds of active mixtures are as one man significantly stronger than baseline mixture.

, can be by using the known activity in other non-elastic materials substitution mixture of different odor characteristic by the present invention Material tests the elastic characteristic of unknown material, and all material exists with threshold concentration.If instead of causing odour intensity phase Dramatically increase more than one unit (in terms of standard benzyl acetate scale) for the mixture of 5 kinds of non-active materials, then it is unknown Material can be designated as elastomeric material according to definition illustrated above.

Fragrance formulations

The above method cannot be only used for test composition and can be used for testing spices.So-called " spices ", refer to that displaying is uniform (if multiaspect) odor property material balance blend.Exist that serve as a variety of smell mixtures of single component, example Such as natural oil；These have the odor property theme of combination, although a series of separate constituent by covering different odor properties Composition.Commercially available spices also generally has clear smell theme, and for this meaning, it can be placed in " spices family tree " and phase The history and practice thus developed for them is discussed.Discuss following aspect spices be it is common, such as：Sweet tea to, flower Fruity；Or pure and fresh, Xin Xiang, Moschus etc..Smell is more complicated and smell has multiagent, but odor property is also tended to uniform etc. Level structure.If spices has the smell face of too many same conspicuousness, it will lose the intuition of the consumer value of spices.Cause This, problem is if as fragrance component to handle what will occur for spices.

The related spices of business tends to show the odor property for being just above threshold value and the odor property in higher concentration Between rational uniformity.Therefore, it can be to handle them a kind of composition such as essential oil such as it, and whether observe it It can be used as elastomeric material.

Spices is not qualified as the complex combination of ten kinds of compositions, but as the single smell with multiple faces.Of short duration Exposure is enough to allow to receive enables subject in some after perceiving first on the enough information of odor characteristics Between carry out spices between useful comparison.Initial exposure can be by strengthening with will be whole the deeper inspection and introspection that perceive characteristic Individual event resolves into potential perception component.This process, which is similar to, perceives purple, then assesses and forms the red and blue of its Relative amount.Absolutely not detract with single sensible form to perceive the ability of blend with parsing the capability analysis of color.

Spices behavior：The measurement of spices elasticity

The code of above-outlined can be used to identify elastomeric material.The code be it is useful because the code use with Material in its threshold concentration incorporation mixture.Its threshold concentration can be used to assess in itself for gained spices, such as can be for essential oil What composition was carried out.Therefore, spices can be mixed in test mixing thing with threshold concentration.

It can lead to the Concentration Testing of the concentration of the main smell breath less than the spices any problem related with mini-components Cross using decreasing concentration measurement series threshold value to minimize.For example, test subject is started and commented with the concentration higher than threshold value Continuous dilution is estimated until flavor profiles are no longer detected.The final concentration for perceiving targeted odorous characteristic is recorded as this and commented The threshold value estimated.Subject should be noted that by using short time sniff to avoid adapting to smell, 2 seconds should be enough, and often rest. Subject can be by repeating the process to confirm threshold value close to some samples of threshold value.Then calculating uniform threshold conduct can be real The concentration of existing 50% verification and measurement ratio.Then the spices for being diluted to uniform threshold is used for such as to the new active substance of other fragrance components Test in.

Methods described is then similar to the method above for test new component.

Experimental section 2：

Evaluation procedure

The plate is equal to experimental section 1, and is based only upon the scale division of identical 8 and by the standard dilutions of benzyl acetate As with reference to the concentration for carrying out evaluate sample.

Sample preparation

Sample is made up of 10mL mixture solution, is placed in the amber powder tanks of 100mL, is covered and is balanced 2+ hours, As described in experimental section 1.

Laboratory sample

Prepare four kinds of laboratory samples：T, u, v and w, it is made up of following：

(t) baseline mixture (t) the only non-active ingredient as known to 5 kinds is made, every kind of to be selected from different non-conterminous smell groups.

(u) pattern (u) of stock blend in a kind of non-active ingredient by the known activity composition from identical smell group (δ-damascone) is prepared in the case of substituting, and obtains the mixture of 4 kinds of non-active ingredients and a kind of active material.

(v) and (w) mixture u is based on three (v) and four (w) formation, wherein the second non-active ingredient is by two kinds of models A kind of substitution in spices：Model 5, consistent with the fragrant and sweet characteristic of main powdery is aesthetically pleasant, and it is by green grass aromatic Enhancing, and model R1, it is developed by model 5, and cooperates with the preparation rule of smell as described above suitable for adaptation.

4 kinds of samples of gained are described in following table ix.

ix：Mixture t, u, v and w formula

Sensory results

ANOVA in unidirectional, subject is carried out to data.Fig. 7 shows the average value figure of mixture t, u, v and x intensity, its Middle error bars represent 95% confidential interval of average value.Significant main influence (F=23.95, df=3, p<0.05) sample is shown Significant difference between product average value is different.The average specific afterwards of Duncan methods relatively shows, all samples average strength that This dramatically different (p<0.05).Mixture w (being made up of model R1) is the best sample of performance, and is significantly stronger than v (by model 5 are made, the variant of identical spices).

x：Duncan is analyzed afterwards

Discussion and conclusion

The experiment shows that mixture w is significantly stronger in all other mixture, and more than one unit stronger than mixture u, And more than two unit stronger than mixture t.Although the concentration of the model R1 spices in incorporation mixture is substantially less than the dense of model 5 The fact that degree is (in mixture v), this is also such.Two kinds of model flavors share similar smell and basis；However, model R1 Adjusted is the rule for meeting above-mentioned elastic spices.Sensory results are consistent with the model R1 for showing as elastic component.Therefore, fall into Spices in being described above is shown as the elastic spices in defined above.

As it is that the spices that mixes in the test of single component has shown that different degrees of elasticity, so as to cause bulk strength Dramatically increase.If these spices are essential oil, it is accredited as elasticity and non-elastic component.As spices, it is believed that it The shared characteristic similar to elastic component, and the performance based on it in the test, it has been identified that elastic spices and non- The presence of elastic spices.This, which can be used for being formed, is subjected to and is suitable for the spices of expected purpose.

Although having described the present invention with reference to specific embodiments of the present invention above, it will be obvious that not departing from this Under conditions of literary disclosed invention design, a variety of changes, modifications and variations can be made.Therefore, it is intended to cover and belongs to institute All such changes in the essence and broad scope of attached claims, modifications and variations.

Claims (4)

1. a kind of method for being used to prepare the composition comprising candidate active component, the described method comprises the following steps：

A. the first inactive ingredients in the first mixture, first inactive ingredients and the candidate active component are selected Odor property is most like；

B. the second inactive ingredients in first mixture, second inactive ingredients and the candidate active group are selected The odor property divided is least similar；

C. the second mixture is prepared, wherein second mixture is identical with first mixture, the difference is that described first Inactive ingredients by equivalent strength the candidate active component replace, and second inactive ingredients by from The known activity material of the second inactive ingredients identical oder levels replaces；

D. the intensity of second mixture is assessed to determine whether second mixture is more significant more than first mixture Strongly, if wherein second mixture is significant stronger than first mixture, then it is assumed that the candidate active component With verified elasticity activity；And e. prepares the flavor compositions for including the candidate active component.

2. according to the method for claim 1, wherein second mixture has than first mixture greatly at least one The intensity of individual intensity fraction unit.

3. a kind of method for the elastic activity level for determining candidate active component, the described method comprises the following steps：

A. the first inactive ingredients in the first mixture are selected, first inactive ingredients have with the candidate active component There is most like odor property；

B. the second inactive ingredients in first mixture, second inactive ingredients and the candidate active group are selected The odor property divided is least similar；

C. the second mixture is prepared, wherein second mixture is identical with first mixture, the difference is that described first Inactive ingredients by equivalent strength the candidate active component replace, and second inactive ingredients by from The known activity material of the second inactive ingredients identical oder levels replaces；

D. the intensity of second mixture is assessed to determine whether second mixture is more significant more than first mixture Strongly, if wherein second mixture is significant stronger than first mixture, then it is assumed that the candidate active component With verified elasticity activity.

4. according to the method for claim 3, wherein second mixture has than first mixture greatly at least one The intensity of individual intensity fraction unit.