CN107427832A - For merging the methods of two liquid volumes, fluidic structures and micro-fluidic chip for implementing this method - Google Patents
For merging the methods of two liquid volumes, fluidic structures and micro-fluidic chip for implementing this method Download PDFInfo
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- CN107427832A CN107427832A CN201680014413.2A CN201680014413A CN107427832A CN 107427832 A CN107427832 A CN 107427832A CN 201680014413 A CN201680014413 A CN 201680014413A CN 107427832 A CN107427832 A CN 107427832A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
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- Automatic Analysis And Handling Materials Therefor (AREA)
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Abstract
The present invention relates to a kind of fluidic structures for being used to control one or more fluids.Fluid circuit (12,32,62,82,122) has the holding section (20,40,70,90) of streamwise (13) extension, fluid circuit has narrow zone (24,44,74,94,134,170) and broad area (26,46,76,96,136,172) adjacent in the horizontal in the holding section, wherein, narrow zone has the minimum side wall spacing h than broad area at least one first direction perpendicular to flow directionwSmaller side wall spacing he.The invention further relates to a kind of method for being used to merge at least two liquid volumes, the first liquid (140) is set to be transported to by fluid circuit in the narrow zone or the broad area that keep section wherein, and then the second liquid (150) separated in advance by buffer medium (146) with the first liquid is transported to and kept at section, and buffer medium through the first liquid by broad area or narrow zone from holding section by exporting.
Description
Technical field
It is the present invention relates to a kind of method for being used for two liquid volumes of merging and a kind of or more for being controlled according to this method
The fluidic structures of kind of fluid, especially microfluidic structures, the fluidic structures have a fluid circuit, fluid circuit limit flow direction and
Each side by side wall limit perpendicular to the cross section of flow direction.The invention further relates to a kind of micro-fluidic chip
(mikrofluidischer Chip, micro-fluid chip), it has substrate, the covering part for substrate and in the substrate this
Kind fluidic structures.Finally, the present invention relates to a kind of system, it is by fluidic structures or micro-fluidic chip together with first fluid and second
Fluid forms, and the method according to the invention makes them merge in fluidic structures or micro-fluidic chip.
Background technology
Such fluidic structures, especially microfluidic structures be used for process part the very small scope in several ml extremely
Amount of liquid in the range of μ l.The lateral dimension that fluid circuit in this structure has is in several mm or lower scope.Liquid
Body is being flowed through in system through processing in this fluidic structures, i.e. is passed through by producing pressure differential (overvoltage and/or negative pressure) conveying
Fluid circuit.On the other hand, in addition to micro-fluidic chip, the technically very high control device of requirement or operation equipment, its
Couple with micro-fluidic chip or insert micro-fluidic chip wherein.
The application of this fluidic structures is of all kinds.By a kind of for example known fluidic structures of the A of document US 59 72 710,
It has diffusion admittance, and diffusion admittance has V-arrangement profile, and sample is merged into parallel laminar flow with detection liquid wherein, so as to
The particle in sample is detected by means of diffusion process.
The A1 of document US 2011/0100476 disclose a kind of micro-fluidic structure, and it has valve, and the valve is by fluid passage
In fusible structure formed.The structure that this is fusible is for enduringly locking fluid to lead in the case of outside energy input
Road.
A kind of valve of microfluidic system is described in the T2 of document DE 600 07 128, it includes the stream with barrier
Body passage.Barrier is formed by intelligent polymer, the intelligent polymer change in the case of outside energy input volume and
Thus the fluid stream by passage is changed.
The A1 of document US 2013/0167958 have studied a kind of micro-fluidic structure in fluid logic pipeline.Micro-fluidic knot
Structure especially includes the branch road for being used to control gas or liquid vesicle in mounting medium.
A kind of micro-fluidic valve of fluid circuit is illustrated in the A1 of document US 2004/0195539, the fluid circuit exists
Constructed in the form of channels in substrate and there is passages shrink portion at the position of valve.Passage utilizes the cover film of tack
Closure.Valve is opened and can persistently closed in the region of contraction flow region by the film pressurization for tack in the initial state.
In addition, illustrate valve can how followed by the base material in the region in passages shrink portion by means of from outside press-in
The deformation of punch-pin is again turned on.
The A1 of document US 2012/0103103427 have studied a kind of micro-fluidic structure with fluid passage, fluid passage
Streamwise is one after the other formed with broad section and narrow section.Flow through two kinds of fluids of fluid passage in the structure shown here parallel
Alternatively concentrate, mix or guided in separated path.
The A1 of document US 2007/0286774 have studied a kind of micro fluidic device with fluid passage, fluid passage construction
In the substrate and covered with film.At least two section is configured with the channel, and liquid is flowed into wherein due to capillarity.Two
Individual section is spaced apart from each other, so as to which very thin stream interrupts between them.Generally better controled over by this structure in passage
Very thin stream.
One in treatment fluid is the aggregation of liquid the problem of special.To such end it is known that such fluid circuit structure,
It leaves portion with least two fluid input lines and one, and they are assembled each other in the region in T-shaped crosspoint.Here,
Difficult point is that should ensure that the liquid column (also known as " liquid plug ") of the confined volume from two intake lines to merge also simultaneously
Reach T-shaped crosspoint.This needs for example to be carried out on a large scale by means of the Vavle switching of complexity and the accurate position monitoring of liquid column
Ground fluid control, control discharge pressure by so and thus adjust the position of liquid column.Position monitoring for example by means of
Grating realizes that grating accurately measures beginning and end of the where in the presence of two liquid columns.In the case of no this regulation,
Gas buffer portion can be between liquid column, it makes the liquid column in fluid circuit be separated from each other all the time.Liquid column due to
Air entrainment and in the case of separating, such as hamper being sufficiently mixed for liquid, or disturb the feature of sensing device.
Elementary object is that control or regulation are restricted in required minimum degree.It should especially avoid at micro-fluidic chip
Complicated precautionary measures, such as movable valve member, because should also pay close attention to alap manufacturing cost herein.
Therefore, the B3 of document DE 10 2,009 048 387 illustrate another approach for merging two kinds of liquid.Wherein say
Bright micro-fluidic structure includes fluid circuit, and it is widened into fluid cavity at the position transverse to flow direction.Fluid cavity
Widen and so obtained with surface, i.e. the first liquid volume guided by fluid cavity is distributed on the whole cross section of fluid cavity.
In addition, another intake line is led in the holding position in fluid cavity, another intake line is constructed so as to, i.e. is conveyed herein
Second liquid volume be parked in the region of holding position, until the first liquid volume for being passed through of the second liquid volume connects
Receive, also, two liquid volumes are exported in a manner of merging from fluid cavity.The structure it is determined that fluid under the premise of (wetting is special
Property) the passive fluid control of replacement a kind of is provided, it can abandon the fluid control of the Vavle switching of complexity and/or other actives
System.
By the written document such as D.Itoh " Droplet-based microfluidic sensing System for
Rapid fish freshness determination " (are published in Sensors and Actuators B171-172
(2012), the 619-626 pages) known to a kind of fluidic structures of all fours.Microfluidic structures described herein include only one
Individual intake line and an output pipe are for handling the liquid column (plug) that two or more are separately conveyed.Liquid column (plug) is logical
Cross intake line and be equally conveyed to the fluid cavity widened in transverse direction apart from each other by bubble, no matter is the volume of fluid cavity
How the volume of first liquid column that reaches is more than.Fluid cavity is constructed so as to, i.e. liquid only wetting phase pair and the sidepiece wall portion put
In one.Thus bypass is discharged, the gas in the gas buffer portion come between comfortable liquid column can be spilt by bypass.Cause
This, in the presence of the discharge pressure of continuation, second liquid post catch up with continue to be adhered to the first liquid at sidepiece wall portion and
Exported therewith in a manner of merging from fluid cavity.However, this another condition needed is that the cumulative volume of two liquid is enough to soak
Two wall portions of the merging chamber widened.
Last-mentioned two kinds of fluidic structures need not be complicated Vavle switching and fluid control and therefore in conception more
Simply, but they have the drawback that, they can not can not in all cases, especially be directed to the liquid and tool of wetability
The liquid for having high surface tension ensures the reliable process of process, because liquid is drawn before tending to due to capillary force or post-tensioning arrives
In narrow fluid pipeline.
The content of the invention
Therefore, it is an object of the invention to provide a kind of simple fluidic structures, it merges logical with being suitable for process
The fluid that buffer medium is spaced conveying of turning up the soil is crossed, the fluid is alternatively more stronger than buffer medium or weaker wetting fluid pipe
The side wall on road.
The purpose passes through the method for the feature with claim 1, the fluidic structures of feature with claim 3, tool
Have the right requirement 16 feature micro-fluidic chip and with claim 21 feature system realize.
It is that the fluid circuits of the fluidic structures being initially mentioned has that streamwise extends, without in addition according to the present invention
Intake line and output pipe holding section, fluid circuit has narrow zone and on flow direction in section is kept
Adjacent broad area in the horizontal, wherein, (first is horizontal at least one first direction perpendicular to flow direction for narrow zone
To direction) on have than broad area minimum side wall spacing hwSmaller side wall spacing he。
Correspondingly, the purpose is also realized by the micro-fluidic chip for the type being initially mentioned, wherein fluid circuit
Construct in the substrate and closed by covering part in the form of channels, wherein, the passage in section is kept is divided into narrow zone
And broad area.It is particularly preferred that here, broad area has the channel depth bigger than narrow zone.The design can
It is extremely simple and be therefore cost-effectively made.Moreover it is preferred that the first horizontal direction is perpendicular to relative with covering part and put
Channel bottom, i.e. the side wall spacing h of narrow zoneeDetermined by channel depth.
Therefore, keeping section to be divided into generally along the longitudinal direction, two adjacent (in transverse direction) transverse to flow direction and fluid connects
The region connect, wherein, a region is narrow at least one direction in space (that is, the first horizontal direction) perpendicular to flow direction
In another region on direction in space.The configuration ensures, the buffer medium than surrounding liquid is weaker or more strongly wetting fluid
The side wall of pipeline and reaching keep the first liquid of section herein in both cases due to capillary force obtain it is reliably solid
It is fixed.Here, also term " in the horizontal " is used to illustrate " transverse to flow direction " or " perpendicular to flow direction ".Illustrate " edge
Also explained with " along longitudinal direction " or " in a longitudinal direction " flow direction ".
Keep section have in fluid circuit in itself in the form of only one intake line and an output pipe conduct
The section of fluid circuit.Two or more liquid columns dividually pass through identical intake line by one or more buffer mediums
In succession be conveyed to keep section and in keeping section merge after by identical output pipe together from holding section from
Open.
Correspondingly, the fluid cavity being quite analogous in document mentioned above, second condition are that holding area is enough
It is fully absorbed within the expected volume V up to first fluid hereinFl1.In other words, it is necessary to the volume V of first fluidFl1Less than guarantor
Hold the volume in region.Alternatively, be referred to as " holding area " is only the narrow zone or broad area for keeping section, according to stream
Where body is based on the stop of its wetting characteristics.Therefore, the volume of holding area can be the volume V for the narrow zone for keeping sectione,
If first fluid is than the side wall of the buffer medium more strongly wetting fluid pipeline of encirclement.What must now be applicable is：VFl1<Ve。
But the volume of holding area can also be the volume V for the broad area for keeping sectionwIf first fluid is than the buffer medium of encirclement
The weaker side wall of wetting fluid pipeline.Therefore what must be applicable is：VFl1<Vw.Only under this condition, first fluid discharges
Keep beginning and the end in the region adjacent in the horizontal of section so that discharge for being trapped among first fluid and following closely through it
Second fluid between buffer medium bypass line.
Third condition is quite analogous to known solution again：The expected cumulative volume of two or more liquid merged
VFl1+VFl2It is enough to close the end for keeping section with liquid.In other words, it is desirable to the liquid V of mergingFl1+VFl2Cumulative volume be more than
The volume of holding area.In the case of the stronger liquid of wetability, the volume V of the narrow zone of section is as kepte：VFl1+
VFl2>Ve.In the case of the weaker liquid of wetability, the volume V of the broad area of section is as keptw：VFl1+VFl2>Vw.Body
Product " VFl2" volume of second liquid or more second liquid is accordingly represented herein.Can make by this way generally two kinds, three kinds or
Others are merged by the separated liquid of buffer medium in holding area, and total liquid volume converged is existed
Exported automatically from the holding area of fluid circuit in the presence of lasting discharge pressure.
These conditions are reflected in the method for being used to merge two liquid volumes according to the present invention.Method provides, is having
Have in the fluidic structures of fluid circuit, fluid circuit limit flow direction and each side by side wall limit perpendicular to flowing side
To cross section and with streamwise extension, holding section without other intake line and output pipe, flow
Body pipeline includes with volume V in the holding sectioneNarrow zone and adjacent in the horizontal on flow direction having
Volume VwBroad area, wherein, narrow zone at least one first direction perpendicular to flow direction have than broad
The minimum side wall spacing h in regionwSmaller side wall spacing he, alternatively, according to wetting characteristics, in step a), will have body
Product VFl1The first liquid by fluid circuit be transported to keep section in and herein because capillary force is transported to narrow zone
In, and and then in step b), separated at least one in advance by buffer medium with the first liquid and there is volume VFl2
Second liquid by identical fluid circuit be transported to keep section in, and buffer medium by by broad area through the first liquid
Body is from section output is kept, until at least one second liquid reaches the first liquid, and side of the two/all liq to merge
Formula exports from holding section, wherein, applicable condition is：VFl1<Ve, and VFl1+VFl2>Ve；Or in step c), there will be body
Product VFl1The first liquid be transported to by fluid circuit and keep section and herein because capillary force is transported to broad area
In, and and then separated at least one in advance by buffer medium with the first liquid and there is volume V in the step d)Fl2
Second liquid by identical fluid circuit be transported to keep section in, and buffer medium by by narrow zone through the first liquid
Body is from section output is kept, until at least one second liquid reaches the first liquid, and side of the two/all liq to merge
Formula exports from holding section, wherein, applicable condition is：VFl1<Vw, and VFl1+VFl2>Vw。
Similarly, these conditions are also reflected in the system according to the present invention, and the system includes the stream of type described above
Body structure or micro-fluidic chip, there is restriction volume VFl1The first liquid, have and limit volume VFl2Second liquid and buffering
Medium, buffer medium are arranged between the first liquid and second liquid in holding area porch and can be with the first liquid and
Two liquid are conveyed by fluid circuit together, wherein, alternatively, the first liquid and second liquid ratio buffer medium more strongly soak
The side wall of fluidic structures, and wherein, applicable condition is：VFl1<Ve, and VFl1+VFl2>Ve, or wherein, the first liquid and
Two liquid are than the side wall of buffer medium weaker wetting fluid structure, and wherein, applicable condition is：VFl1<Vw, and VFl1
+VFl2>Vw。
" liquid is the stronger liquid of wetability " refers to the contact angle that liquid surface is formed with passage surface<It is 90 °, excellent
Selection of land<75 °, and particularly preferably<45°.If the on the contrary, contact angle that liquid surface is formed with passage surface>90 °, preferably
Ground>105 ° and particularly preferably>135 °, liquid is referred to as the weaker liquid of wetability.Referred to as " liquid surface " is the first liquid
The interface of body and second liquid and adjacent buffer medium.In the case of with the interface of gaseous state buffer medium, simplifiedly illustrate
Wetability or non wettability the first liquid and second liquid.Generally, referred to as buffer medium be insoluble in the first liquid and
Medium in second liquid.Medium equally can be gas (such as air) or liquid (such as oil-based fluid, if the first liquid and
Second liquid with water base presence, or vice versa, be water fluid if the first liquid and second liquid are based on oil base).
As application example it should be mentioned that for molecular biology reaction reactant mixture, wherein in aqueous liquid
The nucleic acid as the first liquid and the enzyme as second liquid are conveyed to the fluidic structures according to the present invention in body volume, and
Merged wherein according to the above method, to realize reaction.As feasible buffer medium, mineral oil, silicone oil, fluorinated oil are considered
Or organic polymer (such as " Novec 7500 " (hydrofluoroether (C7F150C2H5)).
Preferably, it is configured with the horizontal stroke in the form of the convex shoulder of streamwise extension between narrow zone and broad area
To transition part.
One or more this convex shoulders can be from the side wall protrusion of one or more limitation fluid circuits.Narrow zone is preferably
Relative configurations convex shoulder plateau and it is relative and between the side wall section put, wherein, the first horizontal direction is perpendicular to plateau.Convex shoulder
There can be sharp or rounding or chamfered edge edge.
There is streamwise to keep entering for section upstream for the preferable design regulation of the present invention, fluid circuit
Enter section (Einlassabschnitt) and streamwise and keep the discharge section in section downstream
(Auslassabschnitt), wherein, holding area is infinitely transitioned into the first lateral direction into section and discharge section
The narrow zone of section is kept in the broad area of section.
In micro-fluidic chip, such as stenosis area is infinitely being transitioned into the channel bottom in section and discharge section
In the channel bottom in domain or the channel bottom of broad area.The channel bottom of narrow zone preferably forms above-mentioned height herein
Platform.
" infinitely (Stufenlos, non-steppedly) " on the one hand includes not having cross section to change ground along the first horizontal direction
It is transitioned into from narrow zone or broad area that holding section is transitioned into section and from there discharge section.This is real one
Apply in mode and be for example achieved in, i.e. accordingly have on the first direction perpendicular to flow direction into section and discharge section
There is side wall spacing hinAnd hout, it is equal to the minimum side wall spacing h of narrow zonee。
In this case, size of the fluid circuit along the first horizontal direction is in the narrow zone in flowing through holding section
Do not change.In the design, the broad area of section is kept to form fluid circuit in the first lateral direction transversal
The those widened sections in face.
" infinitely " transition for the stabilization being additionally included on the other hand between each section.Here, with " stably
(stetig, continuously) " represent continuously, the cross section without mutation change.Therefore, in an alternative embodiment, enter
Section has side wall spacing h in the first lateral directionin>he, wherein, fluid circuit has streamwise in the case where entering section
Swim and keeping the First Transition section of section upstream, horizontal side wall spacing streamwise is from h whereininStably contract
It is small to arrive he。
Similarly, discharge section has side wall spacing h in the first lateral directionout>he, wherein, fluid circuit has edge
Flow direction is in holding section downstream and on the side wall spacing edge of the second transition zone of discharge section upstream, wherein transverse direction
Flow direction is from heStably it is widened to hout。
In the design, the channel cross-section of fluid circuit in the first lateral direction is in approaching side towards holding area
Section narrows down to the side wall spacing of narrow zone, and widens again in the corresponding way in discharge side.Therefore, narrow zone is formed
The contraction flow region of line cross section.
In order to prevent the flowing velocity of liquid from drastically being lifted due to the contraction of line cross section, of the invention one is favourable
Design provides, in the fluid circuit in keeping section in the second direction of flow direction (the second horizontal direction)
Widen in the horizontal relative into section and discharge section.The second advantage widened is can to handle bigger liquid volume, and
The space requirement for the structure not being significantly increased on micro-fluidic chip.By comparison, the passage accordingly extended is being bent in itself
When need more spaces.
Fluid circuit especially can be along the second horizontal direction in the side of narrow zone, in the side of broad area or in both sides
Widen.
Particularly advantageously, broad area in the second direction perpendicular to flow direction relative to enter section and/or
Discharge section dislocation ground arrangement.
The design this have the advantage that, the flowing of fluid is less bright when through the narrow zone kept in section
Show or do not deflect completely, so as to reduce the risk of vortex.
There is streamwise to keep section upstream for another favourable design regulation of the present invention, fluid circuit
And/or at least one stop structure in downstream.
At least one stop structure is preferably configured at least one trend in the side wall for interrupting fluid circuit
The form of convex shoulder.
In the meaning, convex shoulder for example forms one or more of at least one side wall of fluid circuit hollow shape,
Or one or more projections of the formation along at least one side wall of fluid circuit, or form both.Hollow shape can for example lead to
Cross and formed along the passage or recess that laterally set out.Multiple projections can for example form the structure of comb shape.Weigh in all cases
Want, stop structure can not be only crossed in the case of using capillary force.Stop structure prevents the first liquid from flowing into
Exceed to after keeping in section and keep the end of section or because capillary force can be moved into section.Stop structure is with this
Kind mode supports the holding function of narrow zone and make it that the flow process technique when polymerizeing two liquid is more reliable.
Brief description of the drawings
Other advantages of the present invention are illustrated by accompanying drawing below.Wherein：
Fig. 1 a-1c show the first embodiment of the present invention with three views；
Fig. 2 a-2c show second embodiment of the present invention with three views；
Fig. 3 a-3c show third embodiment of the present invention with three views；
Fig. 4 a-4eThe 4th embodiment of the present invention is shown with five views；
Fig. 5 a-5c show three candid photographs for keeping the fluid in section for being flowed into the fluidic structures according to Fig. 1；
Fig. 6 shows the 5th embodiment of the present invention, wherein, design keeping stopping for section upstream in an alternative way
Only structure；
Fig. 7 shows the 6th embodiment of the present invention, wherein, design keeping the stenosis area of section in an alternative way
Convex shoulder between domain and broad area；
Fig. 8 shows the 7th embodiment of the present invention, wherein, design keeping the stenosis area of section in an alternative way
Convex shoulder between domain and broad area；
Fig. 9 a-9c show the 8th embodiment of the present invention with three views；
Figure 10 a-10c show the 9th embodiment of the present invention with three views, and
Figure 11 a-11c show three candid photographs for keeping the fluid in section for being flowed into the fluidic structures according to Fig. 9.
Embodiment
A top view of the first embodiment of the present invention is shown in fig 1 a.Fig. 1 b show longitudinal profile, and
And Fig. 1 c correspondingly show the cross section of the opening position marked in fig 1 a.Show the micro-fluidic core schematically extremely simplified
The substrate 10 of piece, fluid circuit 12 of the only one in the form of passage is configured with wherein.Unshowned fluid is stressed drive
Fluid circuit 12 dynamicly is flowed through along the direction identified by arrow 13, the direction is also known as flow direction or longitudinal direction.Stream
Body pipeline or passage have the cross section defined transverse to flow direction in each side by side wall.Cross section is perpendicular to flowing side
To first party upwardly through channel bottom 14 and one is unshowned, covering at and the position 16 put relative with channel bottom
Portion limits.
In practice, micro-fluidic chip generally has multiple fluid circuits and function element, such as reative cell, mixing knot
Structure, valve etc..In addition, passage is closed in its unlimited upside by means of the film (just covering part) being laminated in substrate.
Fig. 1 a are into 1c, for purposes of simplicity, eliminate the diagram of covering part.
The streamwise of passage 14 is divided into section 18, the holding section 20 in downstream and further downstream discharge area by function
Section 22.
Section 20 is kept laterally to be divided into narrow zone 24 (that is, transverse to flow direction) again and along laterally adjacent therewith
Broad area 26.
Fluid circuit is in narrow zone 24 along the first horizontal direction between channel bottom 14 and covering part (position 16)
With side wall spacing he, it is determined by channel depth in this case.The minimum side wall spacing of broad area 26 passes through hwCome
Represent and extend in the illustrated example along another horizontal direction.As can in Fig. 1 c cross section shown in find out as,
Spacing heLess than the minimum side wall spacing h of broad areaw.The condition is only critical to this：Flow into and keep in section 20
The liquid of (buffer medium for being better than surrounding) moistening be kept due to the capillary force in narrow zone 24, or flow into and protect
Hold buffer medium in section 20, less than surrounding or non-wetting liquid is maintained in broad area 26.It is not in principle
Critical to be, whether the side wall spacing on identical or different direction is equal to each other.Equally it is not important to, narrow zone exists
It is wider than or is narrower than the minimum side wall spacing h of broad area on second horizontal directionw。
The h being shown here ateIt is applicable in the case of being overlapped with channel depth, the channel depth in broad area
(it is more than or equal to minimum side wall spacing hw) channel depth in narrow zone must also be more than.
It will also be seen that there is side wall spacing in the first lateral direction into section in the diagram of Fig. 1 b cross section
hin, and discharge section 22 has side wall spacing hout, and hinAnd houtWith the side in the narrow zone for keeping section 20
Wall spacing heIt is equally big.Therefore, stenosis area is infinitely transitioned into the first lateral direction into section 18 and discharge section 22
In domain 24.In other words, the channel bottom in section and discharge section is entered and in the narrow zone 24 for keeping section 20
14 flatly continue.
And broad area 26 is that starting point forms recess with channel bottom 14.The total depth of broad area 26 is even also greater than
Its width, in this example the width define minimum side wall spacing hw.Due to recess, in narrow zone 24 and broad area
The cross transition portion in the form of the convex shoulder 28 of streamwise extension is formed between 26.Convex shoulder 28 has again in this embodiment
Sharp edge 29.Sharp edge provides bigger reliability of technology in principle, because bigger energy must be utilized to use herein
Flowed in making liquid on edge.For this it is responsible be contact angle hysteresis, it causes the contact line formed by interface and side wall to be stopped
Stay in edge and bending place.On the other hand, for the reason for the process technology, any sharp edge is after all not present, and also
It is functionally nor required.In this sense, term " edge " intentionally includes rounding or chamfered edge edge.
The second embodiment party schematically extremely simplified of the fluidic structures according to the present invention is shown into 2c in Fig. 2 a
Formula.Again, the fluid circuit 32 in the form of passage is configured with the substrate 30 of micro-fluidic chip, fluid circuit is
Closed on one horizontal direction by channel bottom 34,34', and 36 on the upside of it on closed by unshowned covering part or film.
Different from the embodiment according to Fig. 1, the streamwise of fluid circuit 32 has into section 38, First Transition in succession
Section 39, keep section 40, the second transition zone 41 and the discharge section 42 in downstream.Section 40 is kept to be divided laterally into again narrow
Narrow region 44 and along laterally adjacent broad area 46 therewith.Equally, in this broad section 46 with narrow section 44
Channel bottom 34 horizontal plane for starting point formed recess, so as to formed it is between narrow zone 44 and broad area 46,
Horizontal transition part in the form of the convex shoulder 48 with sharp edge 49 of streamwise extension.
It can be seen that in Fig. 2 b longitudinal profile, the side wall between the channel bottom 34' in entering section and upside 36
Spacing hinMore than the side wall spacing h in the narrow zone 44 for keeping section 40e.This is attributable to the level error of channel bottom,
It is bridged in transition zone 39 by the channel bottom 35 of ramped shaped.In other words, thus side wall spacing streamwise 13 from
hinStably taper to he。
Similarly, the side wall spacing h of discharge section 42outMore than the side wall spacing h of narrow zonee, and have herein oblique
The channel bottom 35' of ramp shaped the second transition zone 41 is additionally operable to compensation water adjustment, more precisely, being additionally operable to make side wall spacing
Streamwise is in the second transition zone 41 from heStably it is expanded to hout.Therefore, in this example, into the He of section 38
Discharge section 42 is gone back streamwise and is transitioned into the narrow zone 44 for keeping section 40 without convex shoulder.
Therefore, from the perspective of the fluid of flowing, narrow zone 44 starts to be formed substantially into the cross section of section 38
Cross section shrink, it causes the rising of flowing velocity in the case of constant volume transportation.
In order to avoid such case, fluidic structures can be changed as shown in Fig. 3 a to Fig. 3 c.Sum it is previous
Sample, the embodiment include the micro-fluidic chip with substrate 60, are machined with the fluid circuit in the form of passage in the substrate
62.The fluid that streamwise 13 flows flows therethrough into section 68 again first, and then flows through First Transition section 69, Ran Houliu
Through keeping section 70, the second transition zone 71 also, the last discharge section 72 for flowing through downstream are then flowed through.Keep section 70 again
Being divided laterally into along the first horizontal direction has side wall spacing heNarrow zone 74 and there is minimum side along the longitudinal direction
Wall spacing hwBroad area 76 adjacent therewith.That equally applicable is h hereine<hw.It is in addition, such as same in the embodiment of fig. 2
Sample, here, the side wall spacing h equally in section 68 is enteredinAnd the side wall spacing h in discharge section 72outMore than protecting
Hold the side wall spacing h in the narrow zone 74 of section 70e.Correspondingly, the transition zone 71 of First Transition section 69 and second is distinguished
Channel bottom 65 provided with ramped shaped, 65', they form the transition part without convex shoulder.
Different from the embodiment according to Fig. 2, in the embodiment according to Fig. 3, the fluid circuit 62 in section 70 is kept
Add in the horizontal relative into section 68 and relative to discharge section 72 in the second direction perpendicular to flow direction 13
It is wide.Horizontal those widened sections and the axis of fluid circuit 64 are symmetrical, and broad area 76 is as in two examples before
It is located at the edge of fluid circuit 62 on the second horizontal direction.Therefore, in other words, fluid circuit 62 is in the side of narrow zone
And widen in the side of broad area on the second horizontal direction.
Horizontal those widened sections are advantageous to narrow zone 74 in the following manner first, i.e. it is in a second direction than entering
Section and discharge section are wider.Therefore, because in the first lateral direction from hinTaper to heCross section loss can obtain
To partially compensate for, and flowing velocity can be minimized in narrow zone 74 in the case of constant volume flow.
Meanwhile broad area 76 is relative to entrance section 68 and wrong relative to discharge section 72 on the second horizontal direction
Position ground arrangement.The width of broad area so selects, i.e. its position is in the protuberance by widening cage structure.Therefore, along flowing
Horizontal transition part or convex shoulder 78 that direction 13 extends, between narrow zone 74 and broad area 76 exist with fluid circuit 62
Flushed into the lateral wall portion 79 in section 68 and discharge section 72.This causes the flowing of fluid through in keeping section 70
Generally less drawn away during narrow zone 74.The risk of vortex is significantly reduced from there through the design of those widened sections.
Figure 4 illustrates the design perfect again of fluidic structures.Such as it in the substrate 80 of micro-fluidic chip
The preceding fluid circuit 82 being configured with like that in the form of passage.Fluid circuit 82 has the successive entrance section of streamwise 13
88th, First Transition section 89, holding section 90, the second transition zone 91 and the discharge section 92 in downstream.Section 90 is kept to exist again
It is divided into narrow zone 94 in transverse direction and along laterally adjacent broad area 96 therewith.Here, broad area 96 is also with stenosis area
The level of channel bottom 84 in domain 94 is that starting point forms recess so that construction is between narrow zone 94 and broad area 96
Horizontal transition part in the form of streamwise 13 extends and has the convex shoulder 98 at sharp edge 99.Although sum is previous
Sample, the related side wall spacing h of narrow zone 94eDetermined by channel depth.But it is before different, in broad area 96
Minimum side wall spacing hwDirection overlapped with the first horizontal direction.In other words, between the minimum side wall in broad area 96
Away from hwAlso determined by channel depth herein.Here, equally applicable according to the present invention be：he<hw。
Fluid circuit is used to mend at least in part in transition zone as the horizontal expansion in holding section is before
Repay the diminution of the horizontal line cross section in narrow zone and therefore flowing velocity is reduced herein.Equally, in the reality
Apply in mode and for example this positioning broad area 96, i.e. formed to the convex shoulder 98 of the horizontal transition part of narrow zone 94 with entering
The side wall 100 of section and discharge section flushes.
Different from the embodiment according to Fig. 3, in First Transition section 89, the holding transition zone 91 of section 90 and second
Wall portion 101,102 there is rounding or " can continuous differential " profile.This is advantageous to flow and more prevented in section mistake
Cross and vortex is formed at portion.In addition, this continuous profile makes the boundary being applied between the surface of liquid, gas and passage (solid)
Confining force in contact line between face minimizes, such as the sharp edge 29 in reference chart 1 above and explanation.At this
In at the transition part mentioned, compared to edge 29 discussed above, it is expected side wall contact without the energy cost improved to discharge
Bypass.Therefore, " circle " or " smooth " or " can continuously differential " profile is favourable herein.
Equally, in this embodiment, broader region is not complicated as before.Its approximation has the shape of walking stick,
Wherein, " handle " keep section discharge side end, the end refers to from narrow zone 94.Therefore, broad area 96
The end forms the end 104.If entered in broad area, this is had confirmed to be highly beneficial unintentional liquid.Now prevent
The air cushion being locked at the end 104, i.e. liquid can complete wetting broad area.Therefore, Presence of an interface is always to maintain herein, and its is true
Guarantee in liquid tear starting point and thereby, it is ensured that be emptied completely broad area.
Finally, in this embodiment, the upstream of section 90 is being kept the wall portion put in the relative of fluid circuit 82 to be present
Two stop structures 105,106 in 101 and 102.During stop structure 105,106 is configured in two wall portions 101,102
Empty shape, closed end passage is more specifically configured to, and interrupts the trend of wall portion so that keeping the narrow zone of section 90
The fluid flowed in 94 is not because capillary force is back into section 98 at this.
Fig. 5 a to Fig. 5 c show the order for the liquid flowing in the fluidic structures according to Fig. 1.All three candid photographs are shown
Going out the identical sections of the micro-fluidic chip 110 schematically extremely simplifiedly shown, micro-fluidic chip has substrate 120,
Wherein it is machined with the fluid circuit 122 in the form of passage.Here, the covering part 125 in the form of film is also show now,
It closes laterally unilateral unlimited passage.
Fluid circuit 122 is shown to cutting in the region for keeping section, in the holding section, fluid circuit includes
Narrow zone 134 and the broad area with bigger channel depth adjacent in the horizontal with smaller channel depth
136.The first fluid 140 flowed into along direction 13 has forward position or interface 142, and it is also located at entering area at the time of according to Fig. 5 a
Duan Zhong.
In figure 5b, first fluid 140 is further advanced, so as to can be seen that first fluid enter section in
Interface 144 afterwards.First fluid 140 forms so-called fluid column.Front interface have arrived at the holding section of fluid circuit and by
Entered in capillary force in flat area 134, and it not yet soaks broad area 136.
Buffer medium 146 after liquid column be present, it makes first fluid 140 with subsequent second fluid 150 in space
Upper to separate, second fluid is appeared in into section in fig. 5 c.By continuing on for two fluid columns, at some
The rear interface 144 of one liquid column 140 also reaches the top for the broad area 136 for keeping section.Here, the first liquid column 140
The wall portion where broad area 136 of the interface 144 from fluid passage 122 disengages afterwards.Broad area 136 then discharges bypass pipe
Road, buffer medium 146 can be made by it, spilt insoluble in the gas in first fluid and second fluid or liquid, such as arrow 152
As symbolically showing.At this moment first-class scapus 140 is parked in holding area, because it no longer feels discharge pressure.
Therefore, subsequent liquid column 150 can continue the direction conveying towards the first liquid column 140, until two liquid columns merge.Then,
Both continue to convey together.This can be arranged, i.e. the liquid column of merging is entirely around the air cushion in bypass line, Huo Zheqi
First empty bypass line and and then just leave holding area completely.Thus, the process depends on transition zone
Construction details.
Therefore, the system being related in Figure 5 has：Fluidic structures or micro-fluidic chip, wherein narrow zone 134 have
Volume Ve；First liquid 140, it has the volume V limitedFl1；Second liquid 150, it has the volume V limitedFl2；And buffering
Medium 146, it is arranged between the first liquid and second liquid in the porch of holding area and can be with the first liquid and
Two liquid are transported by fluid circuit 122 together, wherein, the first liquid and second liquid 140,150 are more stronger than buffer medium 146
The side wall of ground wetting fluid structure, and wherein, applicable condition is：VFl1<VeAnd VFl1+VFl2>Ve.If the He of fluid 140
150 be water base, and the side wall of fluid circuit is hydrophilic, and buffer medium 146 for example can be gas or oil.
Figure 6 illustrates the design of the replacement of fluidic structures, and it is corresponded in the vpg connection of fluid circuit 158
Fig. 1 and Fig. 5 scheme.Unique difference is off structure 160, and it has along side wall more specifically along fluid circuit
Multiple projections 162 of channel bottom 163.Projection 162 forms the structure in the comb shape of the end of narrow zone 164 together.
The structure has generally prevented flowing through for the liquid of wetting action, and thus especially prevents liquid inadvertently from holding section
Narrow zone is back in the discharge section of fluid circuit.
Fig. 7 shows simplified micro-fluidic chip 170, wherein, fluid circuit 172 is designed in a manner of alternative, more specifically
Ground says, the convex shoulder of the streamwise extension of the horizontal transition part formed between narrow zone 174 and broad area 176
178.As in the example according to Fig. 4, the side wall spacing h of narrow zone 174eAnd the minimum side of broad area 176
Wall spacing hwDetermined by corresponding channel depth.But all embodiments that convex shoulder 178 is different from showing before are without sharp
Edge, but tool have rounded edges 180.In addition, convex shoulder 178 is also transitioned into the broad area 176 in the form of rounding 184
Channel bottom 182 in.Therefore, transition part has curved cross section, is not mutated or bends, and the effect brought is side
Wall spacing from narrow zone 174 in transverse direction towards broad area 176 by can continuously in the form of differential it is cumulative.
Fig. 8 shows simplified micro-fluidic chip 90, wherein, design fluid circuit 192 in an alternative way again, more really
Say that horizontal transition part, the streamwise to be formed between narrow zone 194 and broad area 196 extends with cutting convex
Shoulder.As in the example according to Fig. 7, the side wall spacing h of narrow zone 194eAnd the minimum side wall of broad area 196
Spacing hwDetermined further through corresponding channel depth, and convex shoulder tool had rounded edges and in leading to from broad area 196
Road bottom 202 has rounding into the transition part of convex shoulder 198.Different from example before or the example according to Fig. 1, broad area
196 be not that starting point forms recess with the channel bottom 203 in narrow zone 196.On the contrary, keep section such shape herein
Into, i.e. narrow zone 194 is that starting point forms eminence or plateau with channel bottom 202.This shape and the reality according to Fig. 2 to Fig. 4
It is close to apply example.However, different, channel bottom 202 passes through First Transition section 209, holding area from into section 208
The broad area 196 of section 210, the second transition zone 211 without transition continue towards discharge section 212 on identical horizontal plane.
In other words, transition zone 209,211 does not extend on whole channel width.
The view of another embodiment of the present invention is shown in a manner of similar to Fig. 1 a in fig. 9 a.Fig. 9 b are correspondingly
Longitudinal profile is shown, and Fig. 9 c correspondingly show the horizontal section of the position marked in fig. 9 a.Show schematically
The extremely substrate 310 of simplified micro-fluidic chip, fluid circuit of the only one in the form of passage is constructed in the substrate
312.Unshowned fluid is stressed along the direction identified by arrow 13 and drivingly flows through fluid circuit 312.Fluid circuit or
Passage has the cross section limited transverse to flow direction in each side by side wall.Cross section is perpendicular to the first of flow direction
Side is upwardly through channel bottom 314 and in unshowned covering part restriction relative with channel bottom and at the position 316 put.
The streamwise of passage 312 again be functionally divided into section 318, downstream holding section 320 and further
The discharge section 322 in downstream.
Section 320 is kept to be divided into narrow zone 324 and along laterally abutting therewith transverse to flow direction 13 in the horizontal, i.e.
Broad area 326.
Fluid circuit (position between channel bottom 314 and covering part in the first lateral direction in narrow zone 324
316) there is side wall spacing he, it is determined by channel depth in this case.The minimum side wall spacing of broad area 326 is led to
Cross hwTo represent and extend in the illustrated example along another horizontal direction.
In as shown in Fig. 9 c cross section it is visible as, spacing heLess than the minimum side wall spacing h of broad areaw。
The condition is only conclusive to this, i.e. will flow into and keeps the liquid for preferably playing wetting action in section due to capillary
Guan Li is maintained in narrow zone 324 or flowed into the liquid for keeping the wetability in section less good and is maintained at broad area
In 326.Equally, be not herein it is conclusive be whether side wall spacing mutually the same on identical or different direction.Equally,
It is not important to, narrow zone is wider than or be narrower than the minimum side wall spacing h of broad area on the second horizontal directionw。
Existing h hereineIt is also especially suitable in the case of being overlapped with channel depth, in broad area 326
Channel depth is more than its width, and width limits the side wall spacing h of minimum in this examplew。
In Fig. 9 a to Fig. 9 c it is also seen that into section 318 and discharge section 322 perpendicular to channel bottom 314 along the
One horizontal direction has side wall spacing hinAnd hout, its channel depth corresponded in the broad area 324 for keeping section 420.
Therefore, infinitely it is transitioned into broad area 324 in the first lateral direction into section 318 and discharge section 322.Change speech
It, channel bottom 314 is flatly prolonged in section and discharge section is entered and in the broad area 324 for keeping section 320
It is continuous.
And narrow zone 326 is in the form of starting point forms the convex shoulder 328 extended in streamwise 13 by channel bottom 314
Horizontal transition part.In this embodiment, convex shoulder 328 has sharp edge 329 again.
In Figure 10 a the top view of another embodiment of the present invention, longitudinal direction are shown into 10c similar to Fig. 9 a to Fig. 9 c
Section and horizontal section.Schematically the substrate of extremely simplified micro-fluidic chip is represented with 410, is configured with wherein only
One fluid circuit 412 in the form of passage.Unshowned fluid is stressed drivingly along the direction identified by arrow 13
Flow through fluid circuit 412.Fluid circuit or passage have the cross section limited transverse to flow direction in each side by side wall.It is horizontal
Section is in the first party perpendicular to flow direction upwardly through channel bottom 414 and in position relative with channel bottom 414 and putting
The unshowned covering part at 416 is put to limit.
Passage 412 be functionally divided into again in the flowing direction into section 418, downstream holding section 420 and enter
The discharge section 422 in one step downstream.
Section 420 is kept to be divided into narrow zone 424 and along laterally abutting therewith transverse to flow direction 13 in the horizontal, i.e.
Broad area 426.
Different from the embodiment according to Fig. 9, the fluid circuit 412 in the embodiment according to Figure 10 is keeping section 420
In be similar to Fig. 3 in example in the second direction perpendicular to flow direction 13 relative to enter section 418 and relative to
Discharge section 422 is horizontal to be widened.Broad area 426 on the second horizontal direction positioned at fluid circuit 412 edge and
Therefore arranged on the second horizontal direction relative into section 418 and relative to the dislocation of discharge section 422.
Horizontal those widened sections are advantageous to narrow zone 424 again, and it is in a second direction than entering section and discharge section more
It is wide.Therefore, because in the first lateral direction from hinNarrow down to heCross section loss can partly be compensated, and narrow
Flowing velocity in narrow region 424 is minimized in the case of constant volume flow.
In addition, fluid circuit with as example in fig.9 in narrow zone 424 in the first lateral direction in passage
There is side wall spacing h between bottom 414 and covering part (position 416)e, it is determined by channel depth.Broad area 426 is most
Small side wall spacing passes through hwTo represent and extend on the second different horizontal directions.
As can in Figure 10 c cross section shown in it is visible as, spacing heLess than between the minimum side wall of broad area
Away from hw.The condition is only conclusive to this, i.e. flows into and keeps the liquid for preferably playing wetting action in section due to hair
Tubule power be maintained in narrow zone 424 or flow into the liquid for keeping the wetability in section less good be maintained at it is broad
In region 426.Equally, be not herein it is conclusive be whether side wall spacing mutually the same on identical or different direction.Together
Sample is not important to, and narrow zone is wider than or be narrower than the minimum side wall spacing of broad area on the second horizontal direction
hw。
Existing h hereineIn the case of being overlapped with channel depth, it is applicable again, the passage in broad area 426
Depth is more than its width, and width limits the side wall spacing h of minimum in this examplew。
In Figure 10 a into 10c it is also seen that into section 318 and discharge section 422 perpendicular to channel bottom 414 the
There is side wall spacing h on one horizontal directioninAnd hout, its passage depth corresponded in the broad area 424 for keeping section 420
Degree.Therefore, infinitely it is transitioned into broad area 424 along the first horizontal direction into section 418 and discharge section 422.Change speech
It, channel bottom 414 is flatly prolonged in section and discharge section is entered and in the broad area 424 for keeping section 420
It is continuous.
Narrow zone 426 is again the shape that starting point forms the convex shoulder 428 extended in streamwise 13 with channel bottom 414
The horizontal transition part of formula.In this embodiment, convex shoulder 428 also has sharp edge 429 again.
Figure 11 a to Figure 11 c are similar to Fig. 5 a to Fig. 5 c and show incoming fluid structure, are specifically fluid knot according to Fig. 9
The order of liquid in structure.All three, which capture, shows that what is schematically extremely simplifiedly shown has the micro- of substrate 520
The identical section of fluidic chip 510, the fluid circuit 522 in the form of passage is machined with the substrate.Here, again show
Covering part 525 in the form of film, it closes unilateral unlimited passage in the horizontal.
Fluid circuit 522 is shown with section in the region for keeping section, in the holding section, fluid circuit includes
Narrow zone 534 and the broad area with bigger channel depth adjacent in the horizontal with smaller channel depth
536.The first fluid 540 flowed into along direction 13 has forward position or interface 542, and it is still located at entering at the time of according to Figure 11 a
In section.
In Figure 11 b, first fluid 540 continues on, so as to it can see first fluid in section is entered after
Interface 544.First fluid 540 forms so-called fluid column.Front interface 542 have arrived at the holding section of fluid circuit and by
Enter in capillary force in broad area 536, and it avoids narrow zone 534.Capillary force is in this case due to opposite
The situation that wetting characteristics is different from Fig. 5 works.
Buffer medium 546 be present at liquid column rear, its spatially separated first fluid 540 and subsequent second fluid
550, in Figure 11 c enter section in visible second fluid.At some, pass through the further row of two fluid columns
Enter, the rear interface 544 of the first liquid column 540 also reaches the beginning for the broad area 536 for keeping section.Here, the first liquid column
540 rear interface 544 disengages from the wall portion of fluid passage 522, narrow zone 534 at the wall portion be present.Narrow zone 534 connects
And discharge bypass line, buffer medium 546 can be spilt by bypass line, as arrow 552 symbolically represents.This
When, first-class scapus 540 is parked in holding area, because it no longer experiences discharge pressure.Therefore, subsequent liquid column 550
The direction conveying towards the first liquid column 540 can be continued, until two liquid columns merge.Then both are made to continue to convey together.This
It can be arranged, i.e. the liquid column of merging is advanced completely around the air cushion in bypass line, or the liquid column merged is first
Bypass line is emptied and and then just leaves holding area completely.Thus, the process depends on the structure of transition zone
The details made.
Therefore, the system being related in example in fig. 11 has：Fluidic structures or micro-fluidic chip, wherein broad area
Domain 536 has volume Vw；First liquid 540, it has the volume V limitedFl1；Second liquid 550, it has the volume limited
VFl2；With buffer medium 546, it is arranged between the first liquid and second liquid in the porch of holding area and can be with
One liquid and second liquid convey by fluid circuit 522 together, wherein, the first liquid 540 and second liquid 550 are situated between than buffering
The side wall of the weaker wetting fluid structure of matter 546, and wherein, applicable condition is：VFl1<Vw, and VFl1+VFl2>VW.If
Fluid 540 and 550 is water base plinth, and the side wall of fluid circuit is hydrophobic, and buffer medium 546 for example can be gas or oil.
Reference numerals list
10 substrates
12 fluid circuits/passage
13 flow directions
14 channel bottoms
The upside of 16 passages
18 enter section
20 keep section
22 discharge sections
24 narrow zones
26 broad areas
28 convex shoulders
29 edges
30 substrates
32 fluid circuits
34 channel bottoms
34 ' channel bottoms
The channel bottom of 35 ramped shapeds
35 ' the channel bottoms of ramped shaped
The upside of 36 passages
38 enter section
39 First Transition sections
40 keep section
41 second transition zones
42 discharge sections
44 narrow zones
46 broad areas
48 convex shoulders
49 edges
60 substrates
62 fluid circuits/passage
The channel bottom of 65 ramped shapeds
65 ' the channel bottoms of ramped shaped
68 enter section
69 transition zones
70 keep section
71 second transition zones
72 discharge sections
74 narrow zones
76 broad areas
78 convex shoulders
The lateral wall portion of 79 fluid circuits
80 substrates
82 fluid circuits/passage
84 channel bottoms
88 enter section
89 transition zones
90 keep section
91 second transition zones
92 discharge sections
94 narrow zones
96 broad areas
98 convex shoulders
99 edges
100 enter the lateral wall portion of section and discharge section
101 wall portion in transition zone and holding section
102 wall portion in transition zone and holding section
The end of 104 broad areas
105 stop structures/hollow shape/closed end passage
106 stop structures/hollow shape/closed end passage
110 micro-fluidic chips
120 substrates
122 fluid circuits
125 covering parts/film
134 narrow zones
136 broad areas
140 first fluids/fluid column
The front interface of 142 first-class scapus
The rear interface of 144 first-class scapus
146 buffer mediums
150 second fluids/fluid column
152 flow arrows
158 fluid passages
160 stop structures
162 projections
163 channel bottoms
164 narrow zones
170 micro-fluidic chips
172 fluid circuits/passage
174 narrow zones
176 broad areas
178 convex shoulders
180 edges
The channel bottom of 182 broad areas
184 roundings
190 micro-fluidic chips
192 fluid circuits/passage
194 narrow zones
196 broad areas
202 (in broad area) channel bottoms
203 channel bottom in narrow zone
208 enter section
209 First Transition sections
210 keep section
211 second transition zones
212 discharge sections
310 substrates
312 fluid circuits/passage
314 channel bottoms
The upside of 316 passages
318 enter section
320 keep section
322 discharge sections
324 narrow zones
326 broad areas
328 convex shoulders
329 edges
410 substrates
412 fluid circuits/passage
414 channel bottoms
The upside of 416 passages
418 enter section
420 keep section
422 discharge sections
424 narrow zones
426 broad areas
428 convex shoulders
429 edges
510 micro-fluidic chips
520 substrates
522 fluid circuits
525 covering parts/film
534 narrow zones
536 broad areas
540 first fluids/fluid column
The front interface of 542 first-class scapus
The rear interface of 544 first-class scapus
546 buffer mediums
550 second fluids/fluid column
552 flow arrows
Claims (21)
1. one kind is used in the fluidic structures with fluid circuit (12,32,62,82,122,172,192,312,412,522)
Merge the method for at least two liquid volumes, the fluid circuit limit flow direction (13) and limited in each side by side wall,
Perpendicular to the cross section of the flow direction (13) and with streamwise (13) extension, without other intake line
With the holding section (20,40,70,90,210,320,420) of output pipe, the fluid circuit (12,32,62,82,122,
172nd, 192,312,412,522) include in the holding section with volume VeNarrow zone (24,44,74,94,134,
174th, 194,324,424,534) and adjacent in the horizontal on flow direction there is volume VwBroad area (26,46,
76th, 96,136,176,196,326,426,536), wherein, the narrow zone (24,44,74,94,134,164,174,194,
324th, 424,534) have at least one first direction perpendicular to the flow direction (13) than the broad area
The minimum side wall spacing h of (26,46,76,96,136,176,196,326,426,536)wSmaller side wall spacing he, this method
Alternatively have steps of：
A) there will be volume VFl1The first liquid (140) by fluid circuit (122) be transported to it is described holding section in, and
Herein because capillary force is transported in the narrow zone (134),
B) it is and then will being separated in advance by buffer medium (146) with first liquid (140), there is volume VFl2Extremely
A kind of few second liquid (150) is transported in the holding section by identical fluid circuit (122), and the buffering is situated between
Matter (146) exports by the way that the broad area (136) is other through first liquid (140) from the holding section, until described
At least one second liquid (150) reaches first liquid (140), and make two kinds or all liq in a manner of merging from
The holding section output, wherein, applicable condition is：
VFl1<Ve, and VFl1+VFl2>Ve,
Or
C) there will be volume VFl1The first liquid (540) by the fluid circuit (522) be transported to it is described holding section in simultaneously
And herein because capillary force is transported in the broad area (536),
D) it is and then will being separated in advance by buffer medium (546) with first liquid (540), there is volume VFl2Extremely
A kind of few second liquid (550) is transported in the holding section by identical fluid circuit (522), and the buffering is situated between
Matter (546) exports by the way that the narrow zone (534) is other through first liquid (540) from the holding section, until described
At least one second liquid (550) reaches first liquid (540), and make two kinds or all liq in a manner of merging from
The holding section output, wherein, applicable condition is：VFl1<Vw, and VFl1+VFl2>Vw。
2. according to the method for claim 1, it is characterised in that the buffer medium (146,546) is gas buffer medium.
3. a kind of fluidic structures, it is adapted to the one or more streams of method control according to any one of the claims
Body, the fluidic structures have fluid circuit (12,32,62,82,122,172,192,312,412,522), and the fluid circuit limits
Flow direction (13) and limited in each side by side wall, perpendicular to the cross section of flow direction (13), it is characterised in that it is described
Fluid circuit (12,32,62,82,122,172,192,312,412,522) has streamwise (13) extension, no another
The holding section (20,40,70,90,210,320,420) of outer intake line and output pipe, the fluid circuit (12,32,
62nd, 82,122,172,192,312,412,522) have in the holding section narrow zone (24,44,74,94,134,174,
194th, 324,424,534) and on the flow direction abut in the horizontal broad area (26,46,76,96,136,176,
196th, 326,426,536), wherein, the narrow zone (24,44,74,94,134,164,174,194,324,424,534) exists
On at least one first direction of the flow direction (13) have than the broad area (26,46,76,96,136,
176th, 196,326,426, minimum side wall spacing h 536)wSmaller side wall spacing he。
4. fluidic structures according to claim 3, it is characterised in that be configured with the narrow zone (24,44,74,
94th, 134,164,174,194,324,424,534) and the broad area (26,46,76,96,136,176,196,326,
426th, 536) between, in streamwise (13) extension convex shoulder (28,48,78,98,178,328,428) in the form of transverse direction
Transition part.
5. fluidic structures according to claim 4, it is characterised in that the narrow zone (24,44,74,94,134,
164th, 174,194,324,424,534) construction the convex shoulder (28,48,78,98,178,328,428) plateau and it is relative and
Between the side wall section put, wherein, the first direction is perpendicular to the plateau.
6. the fluidic structures according to any one of claim 3 to 5, it is characterised in that the fluid circuit (12,32,
62nd, 82,122,172,192,312,412,522) have streamwise (13) the holding section (20,40,70,90,
210th, 320,420) upstream enters section (18,38,68,88,208,318,418) and streamwise (13) in the holding
The discharge section (22,42,72,92,212,322,422) in section (20,40,70,90,210,320,420) downstream, wherein, institute
State and enter section (18,38,68,88,208,318,418) and the discharge section (22,42,72,92,212,322,422) edge
First direction be infinitely transitioned into it is described holding section (20,40,70,90,210) narrow zone (24,44,74,94,134,
164th, 174,194) or in the broad area (176,196,326,426,536) for keeping section (210,320,420).
7. fluidic structures according to claim 6, it is characterised in that described to enter section (18) and/or the discharge area
Section (22) has side wall spacing h on the first direction perpendicular to the flow direction (13)inAnd hout, it is equal to described narrow
The side wall spacing h in region (24)e。
8. fluidic structures according to claim 6, it is characterised in that the section (38,68,88,208) that enters is vertical
In having side wall spacing h on the first direction of the flow direction (13)in>he, and the fluid circuit (32,62,82,
192) have streamwise (13) it is described enter section (38,68,88,208) downstream and it is described holding section (40,
70th, 90,210) the First Transition section (39,69,89,209) of upstream, in the transition zone, side wall spacing streamwise
(13) from hinStably narrow down to he。
9. the fluidic structures according to any one of claim 6 or 8, it is characterised in that the discharge section (42,72,
92nd, 212) there is side wall spacing h along the first direction perpendicular to the flow direction (13)out>he, and the fluid circuit
(32,62,82,192) there is streamwise (13) in holding section (40,70, the 90,210) downstream and in the row
Go out second transition zone (41,71,91,211) of section (42,72,92,212) upstream, in second transition zone, side wall
Spacing streamwise (13) is from heStably it is widened to hout。
10. the fluidic structures according to any one of claim 3 to 9, it is characterised in that the fluid circuit (62,82,
412) have streamwise (13) it is described holding section (70,90,420) upstream enter section (68,88,418) and
Discharge section (72,92,422) with streamwise (13) in described holding section (70,90,420) downstream, wherein, institute
Fluid circuit (62,82,412) is stated in the holding section (70,90,420) in the second party perpendicular to flow direction (13)
Widen in the horizontal into section (68,88,418) and the discharge section (72,92,422) relative to described upwards.
11. fluidic structures according to claim 10, it is characterised in that the broad area (76,96,426) is vertical
In entering section (68,88,418) and/or relative to the row relative to described in the second direction of the flow direction (13)
Go out section (72,92,422) dislocation ground arrangement.
12. the fluidic structures according to any one of claim 3 to 11, it is characterised in that the fluid circuit (82,
158) have streamwise (13) it is described holding section (90) upstream and/or downstream at least one stop structure (105,
106、160)。
13. fluidic structures according to claim 12, it is characterised in that at least one stop structure (105,106,
160) it is configured in the form of the convex shoulder for interrupting the trend of at least one side wall of the fluid circuit (82,158).
14. fluidic structures according to claim 13, it is characterised in that at least one stop structure (105,106)
The hollow shape being configured at least one side wall of the fluid circuit (82).
15. fluidic structures according to claim 13, it is characterised in that at least one stop structure (160) construction
For along the projection (162) of at least one side wall of the fluid circuit (158).
16. a kind of micro-fluidic chip (10,170,190,510), including substrate (10,30,60,80,120,310,410,520),
For the substrate (10,30,60,80,120,310,410,520) covering part (125,525) and the substrate (10,30,
60th, 80,120,310,410,520) in the fluidic structures according to any one of claim 3 to 15, it is characterised in that
The fluid circuit (12,32,62,82,122,172,192,312,412,522) be configured to be in the substrate (10,30,60,
80th, 120,310,410,520) in passage form and closed by the covering part (125,525), wherein, the passage
Be divided into holding section (20,40,70,90,210,320,420) narrow zone (24,44,74,94,134,164,
174th, 194,324,424,534) and broad area (26,46,76,96,136,176,196,326,426,536).
17. micro-fluidic chip (110,170,190,510) according to claim 16, it is characterised in that the broad area
Domain (26,46,76,96,136,176,196,326,426,536) have than the narrow zone (24,44,74,94,134,
164th, 174,194,324,424,534) bigger channel depth.
18. the micro-fluidic chip (110,170,190,510) according to claim 16 or 17, it is characterised in that described
One direction perpendicular to it is relative with the covering part (125,525) and put channel bottom (14,34,34', 84,163,202,203,
314、414)。
19. micro-fluidic chip (110,170,190,510) according to claim 18, it is characterised in that the channel floor
Portion (14,34', 84,63,202,314,414) streamwise in section (18,38,68,88,208,318,418) is entered
(13) it is described holding section (20,40,70,90,210,320,420) upstream and discharge section (22,42,72,92,
212nd, 322,422) in streamwise (13) in the holding section (20,40,70,90,210,320,420), downstream is infinitely
Be transitioned into the narrow zone (24,44,74,94,134,164,174,194) channel bottom or the broad area (176,
196th, 326,426, in channel bottom 536).
20. micro-fluidic chip (110,170,190,510) according to claim 19, it is characterised in that the stenosis area
The channel bottom in domain (24,44,74,94,134,164,174,194,324,424,534) is formed in the sense that claim 5
Plateau.
21. a kind of system, have fluidic structures according to any one of claim 3 to 15 or according to claim 16 to
Any one of 20 micro-fluidic chip (110,510), wherein, narrow zone (134,534) has volume Ve, and broad area
(136,536) there is volume Vw, the system includes:
Volume V with restrictionFl1The first liquid (140,540), have limit volume VFl2Second liquid (150,
550),
With buffer medium (146,546), its keep section porch be arranged in first liquid and the second liquid it
Between and can be conveyed together with first liquid and the second liquid by the fluid circuit (122,522),
Wherein, alternatively
Buffer medium described in-first liquid and second liquid ratio more strongly soaks the side wall of the fluidic structures, and
And wherein, applicable condition is：VFl1<Ve, and VFl1+VFl2>Ve,
Or
Buffer medium described in first liquid and second liquid ratio weaker soaks the side wall of the fluidic structures, and
Wherein, applicable condition is：VFl1<Vw, and VFl1+VFl2>Vw。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102015204235.9A DE102015204235B4 (en) | 2015-03-10 | 2015-03-10 | Fluidic structure with holding section and method for uniting two fluid volumes |
| DE102015204235.9 | 2015-03-10 | ||
| PCT/EP2016/000429 WO2016142067A1 (en) | 2015-03-10 | 2016-03-10 | Method for combining two fluid volumes, fluidic structure and microfluidic chip for carrying out said method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107427832A true CN107427832A (en) | 2017-12-01 |
| CN107427832B CN107427832B (en) | 2020-10-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201680014413.2A Active CN107427832B (en) | 2015-03-10 | 2016-03-10 | Fluid structure and micro-fluidic chip and system comprising same |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP3268130B1 (en) |
| CN (1) | CN107427832B (en) |
| DE (1) | DE102015204235B4 (en) |
| WO (1) | WO2016142067A1 (en) |
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| KR102224729B1 (en) | 2016-12-23 | 2021-03-08 | 라디오미터 메디컬 에이피에스 | Multi-use body fluid sensor assembly |
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| WO2013160408A2 (en) * | 2012-04-25 | 2013-10-31 | Scope Fluidics Sp. Z O.O. | Microfluidic device |
| CN103537329A (en) * | 2012-07-11 | 2014-01-29 | 三星电子株式会社 | Microfluidic structure, microfluidic device having the same and method of controlling the microfluidic device |
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| US5716852A (en) * | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
| SE9902474D0 (en) * | 1999-06-30 | 1999-06-30 | Amersham Pharm Biotech Ab | Polymer valves |
| US6935617B2 (en) * | 2002-07-26 | 2005-08-30 | Applera Corporation | Valve assembly for microfluidic devices, and method for opening and closing the same |
| DE10302720A1 (en) * | 2003-01-23 | 2004-08-05 | Steag Microparts Gmbh | Microfluidic switch for stopping the flow of fluid during a time interval |
| WO2006061026A2 (en) * | 2004-12-09 | 2006-06-15 | Inverness Medical Switzerland Gmbh | A micro fluidic device and methods for producing a micro fluidic device |
| US7918244B2 (en) * | 2005-05-02 | 2011-04-05 | Massachusetts Institute Of Technology | Microfluidic bubble logic devices |
| KR101097357B1 (en) * | 2009-07-09 | 2011-12-23 | 한국과학기술원 | Multi function microfluidic flow control apparatus and multi function microfluidic flow control method |
| DE102009048378B3 (en) * | 2009-10-06 | 2011-02-17 | INSTITUT FüR MIKROTECHNIK MAINZ GMBH | Microfluidic structure |
| KR101130698B1 (en) * | 2009-11-03 | 2012-04-02 | 삼성전자주식회사 | Valve unit, microfluidic device including the same, and driving method of the valve unit |
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2015
- 2015-03-10 DE DE102015204235.9A patent/DE102015204235B4/en active Active
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2016
- 2016-03-10 WO PCT/EP2016/000429 patent/WO2016142067A1/en active Application Filing
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101180540A (en) * | 2005-03-29 | 2008-05-14 | 因弗因斯医药瑞士股份有限公司 | Hybrid device |
| WO2013160408A2 (en) * | 2012-04-25 | 2013-10-31 | Scope Fluidics Sp. Z O.O. | Microfluidic device |
| CN103537329A (en) * | 2012-07-11 | 2014-01-29 | 三星电子株式会社 | Microfluidic structure, microfluidic device having the same and method of controlling the microfluidic device |
Also Published As
| Publication number | Publication date |
|---|---|
| DE102015204235A1 (en) | 2016-09-15 |
| EP3268130B1 (en) | 2021-05-26 |
| CN107427832B (en) | 2020-10-02 |
| WO2016142067A1 (en) | 2016-09-15 |
| EP3268130A1 (en) | 2018-01-17 |
| DE102015204235B4 (en) | 2016-12-15 |
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