CN107422760A - A kind of cell constant temperature device for micro- sem observation - Google Patents
A kind of cell constant temperature device for micro- sem observation Download PDFInfo
- Publication number
- CN107422760A CN107422760A CN201710673099.5A CN201710673099A CN107422760A CN 107422760 A CN107422760 A CN 107422760A CN 201710673099 A CN201710673099 A CN 201710673099A CN 107422760 A CN107422760 A CN 107422760A
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- temperature
- plate
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- sem observation
- cell constant
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of cell constant temperature device for micro- sem observation, including temperature control box, the temperature control box are made up of bottom plate, top plate and hollow plate, and sample mounting table and Both wide field illumination window are provided with the bottom plate and top plate；Temperature-adjusting heating device is arranged with the bottom plate, top plate and hollow plate madial wall, temperature-adjusting heating device is electrically connected with temperature controller, and temperature sensor is provided with the bottom plate, and temperature sensor electrically connects with temperature controller.The beneficial effects of the invention are as follows：First, temperature stabilization in temperature control box, its temperature control precision is high；Second, temperature can be realized and automatically controlled, and need to only be put into sample, design temperature, have the advantages of simple to operate, have certain push rod application value.
Description
Technical field
The present invention relates to light microscope technique field, and in particular to a kind of cell constant temperature for micro- sem observation fills
Put.
Background technology
Living cells imaging is a kind of to observe cell interior knot by dynamic in real time or the IMAQ in a period of time
The research method of structure motion change and cellular physiological processes.On the one hand living cells imaging will keep the physiologically active of cell, another
Aspect can be applicable microscope and carry out Real Time Observation and IMAQ, it is therefore desirable to a kind of dress for being able to maintain that cell life environment
Put.Under the conditions of unsuitable temperature etc., cell is typically only capable to maintain the normal activity of short time.Therefore it is real on microscope
Existing living cells imaging, it is necessary to using a kind of device for maintaining cell constant temperature, to maintain the normo-metabolic of cell.
During the present invention is realized, applicants have appreciated that following technological deficiency be present in currently available technology：
1. still temperature control precision is poor for cell constant temperature device small volume.
2. cell constant temperature device complete function, but volume is larger, it is more difficult that complex operation is safeguarded.
The content of the invention
It is an object of the invention to provide a kind of cell constant temperature device for micro- sem observation, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the present invention provides following technical scheme：
A kind of cell constant temperature device for micro- sem observation, including temperature control box, the temperature control box is by bottom plate, top plate and returns
Template is formed, and sample mounting table and Both wide field illumination window are provided with the bottom plate and top plate；The bottom plate, top plate and return
Temperature-adjusting heating device is arranged with template madial wall, temperature-adjusting heating device electrically connects with temperature controller, is set on the bottom plate
Temperature sensor is equipped with, temperature sensor electrically connects with temperature controller.
It is as further scheme of the invention：The temperature control box is the shell of closed.
As the present invention, further scheme is：Insulation material is posted on the hollow plate madial wall.
As the present invention, further scheme is：Some apertures are offered in the hollow plate side wall.
As the present invention, further scheme is：The temperature-adjusting heating device includes water-storing tray, and water is filled in water-storing tray,
And heater strip is provided with water-storing tray.
As the present invention, further scheme is：The temperature controller is electrically connected with the controller.
The beneficial effects of the invention are as follows：
First, temperature stabilization in temperature control box, its temperature control precision is high；
Second, temperature can be realized and automatically controlled, and only need to be put into sample, design temperature, be had simple to operate excellent
Point, there is certain application value.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Referring to Fig. 1, in the embodiment of the present invention, a kind of cell constant temperature device for micro- sem observation, including temperature control box
1, the temperature control box 1 is made up of bottom plate 101, top plate 103 and hollow plate 102, and bottom plate 101 can be fixed on microscope slide translation stage
On, sample mounting table and Both wide field illumination window are provided with the bottom plate 101 and top plate 103, the sample mounting table of bottom is used
Sample is placed in inverted microscope, the sample mounting table at top is applied to stereomicroscope and places sample；The bottom plate 101, top
Temperature-adjusting heating device is arranged with plate 103 and the madial wall of hollow plate 102, temperature-adjusting heating device is electrically connected with temperature controller 5
Connect, temperature sensor 6 is provided with the bottom plate 101, temperature sensor 6 electrically connects with temperature controller 5.
The temperature control box 1 is the shell of closed.
Insulation material is posted on the madial wall of hollow plate 102.
Some apertures are offered in the side wall of hollow plate 102, are easy to air to enter.
The temperature-adjusting heating device includes water-storing tray 7, and water is filled in water-storing tray 7, and heater strip 4 is provided with water-storing tray 7,
When temperature is controlled in temperature control box 1, the temperature inside temperature control box 1 is monitored using temperature sensor 6, temperature passes
Sensor 6 passes data to temperature controller 5, and when temperature is less than setting value, signal is passed to temperature control by temperature sensor 6
Device processed, temperature sensor 6 control the work of heater strip 4, and heater strip 4 produces heat, the water in water-storing tray 7 is carried out at heating
Reason, when the temperature in temperature control box 1 reaches setting value, temperature sensor 6 passes data to temperature controller 5, temperature control
Device 5 makes heater strip 4 be stopped.
The temperature controller 5 is electrically connected with the controller.
The present invention operation principle be：When temperature is controlled in temperature control box 1, using temperature sensor 6 to temperature control
Temperature inside case 1 is monitored, and temperature sensor 6 passes data to temperature controller 5, when temperature is less than setting value,
Signal is passed to temperature controller by temperature sensor 6, and temperature sensor 6 controls the work of heater strip 4, and heater strip 4 produces heat
Amount, heats to the water in water-storing tray 7, and when the temperature in temperature control box 1 reaches setting value, temperature sensor 6 is by number
According to temperature controller 5 is passed to, temperature controller 5 makes heater strip 4 be stopped.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (6)
1. a kind of cell constant temperature device for micro- sem observation, including temperature control box, it is characterised in that the temperature control box is the bottom of by
Plate, top plate and hollow plate are formed, and sample mounting table and Both wide field illumination window are provided with the bottom plate and top plate；The bottom
Temperature-adjusting heating device is arranged with plate, top plate and hollow plate madial wall, temperature-adjusting heating device electrically connects with temperature controller,
Temperature sensor is provided with the bottom plate, temperature sensor electrically connects with temperature controller.
A kind of 2. cell constant temperature device for micro- sem observation according to claim 1, it is characterised in that the temperature control
Case is the shell of closed.
A kind of 3. cell constant temperature device for micro- sem observation according to claim 1, it is characterised in that the hollow
Insulation material is posted on plate madial wall.
4. a kind of cell constant temperature device for micro- sem observation according to claim 1 or 3, it is characterised in that described
Some apertures are offered in hollow plate side wall.
A kind of 5. cell constant temperature device for micro- sem observation according to claim 1, it is characterised in that the temperature
Heater includes water-storing tray, fills water in water-storing tray, and heater strip is provided with water-storing tray.
6. a kind of cell constant temperature device for micro- sem observation according to claim 1 or 5, it is characterised in that described
Temperature controller is electrically connected with the controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710673099.5A CN107422760A (en) | 2017-08-09 | 2017-08-09 | A kind of cell constant temperature device for micro- sem observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710673099.5A CN107422760A (en) | 2017-08-09 | 2017-08-09 | A kind of cell constant temperature device for micro- sem observation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107422760A true CN107422760A (en) | 2017-12-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710673099.5A Pending CN107422760A (en) | 2017-08-09 | 2017-08-09 | A kind of cell constant temperature device for micro- sem observation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342319A (en) * | 2018-04-20 | 2018-07-31 | 内蒙古民族大学 | The constant temperature incubation device of microscopically observation cell |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101992894A (en) * | 2009-08-28 | 2011-03-30 | 中国航天员科研训练中心 | Portable cell sample conveying box |
| EP2311935A1 (en) * | 2008-07-29 | 2011-04-20 | Kyoto University | Tissue-derived biomaterial carrier device |
| CN103336540A (en) * | 2013-06-09 | 2013-10-02 | 沈阳创达技术交易市场有限公司 | Control circuit of constant temperature incubator |
| CN204676090U (en) * | 2015-05-12 | 2015-09-30 | 重庆市大足区珠溪中心卫生院 | A kind of inspection box for cell cultures |
| CN205193635U (en) * | 2015-12-14 | 2016-04-27 | 徐州医学院 | Portable culture medium heater |
-
2017
- 2017-08-09 CN CN201710673099.5A patent/CN107422760A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2311935A1 (en) * | 2008-07-29 | 2011-04-20 | Kyoto University | Tissue-derived biomaterial carrier device |
| CN101992894A (en) * | 2009-08-28 | 2011-03-30 | 中国航天员科研训练中心 | Portable cell sample conveying box |
| CN103336540A (en) * | 2013-06-09 | 2013-10-02 | 沈阳创达技术交易市场有限公司 | Control circuit of constant temperature incubator |
| CN204676090U (en) * | 2015-05-12 | 2015-09-30 | 重庆市大足区珠溪中心卫生院 | A kind of inspection box for cell cultures |
| CN205193635U (en) * | 2015-12-14 | 2016-04-27 | 徐州医学院 | Portable culture medium heater |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342319A (en) * | 2018-04-20 | 2018-07-31 | 内蒙古民族大学 | The constant temperature incubation device of microscopically observation cell |
| CN108342319B (en) * | 2018-04-20 | 2021-07-16 | 内蒙古民族大学 | Constant temperature culture apparatus for observing cell under microscope |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171201 |
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| WD01 | Invention patent application deemed withdrawn after publication |