CN107384798B - Preservation method of filamentous algae - Google Patents
Preservation method of filamentous algae Download PDFInfo
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- CN107384798B CN107384798B CN201710586573.0A CN201710586573A CN107384798B CN 107384798 B CN107384798 B CN 107384798B CN 201710586573 A CN201710586573 A CN 201710586573A CN 107384798 B CN107384798 B CN 107384798B
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The invention provides a preservation method of filamentous algae, which comprises the following steps: reducing the water content of filamentous algae to a preset value to obtain algae mud; adjusting the thickness of the algae mud to a preset thickness, sealing, and precooling for a first preset time at a first preset temperature; and (4) placing the precooled algae mud at a second preset temperature for a second preset time. According to the invention, the water content of filamentous algae is reduced to a preset value, so that the water activity of filamentous algae is reduced, the respiration consumption of algae cells is reduced in a short period, the intensity of life activities is reduced, the filamentous algae is precooled before being frozen and stored, the damage degree of the activity of the algae cells during being frozen and stored is reduced, and the cell activity of the filamentous algae during being stored is increased; and the growth of most pollutants can be inhibited, a large amount of high-activity algae seeds can be provided in a short period of time, and the algae seeds can be immediately put into production after being transported to a destination, so that the production efficiency is improved. Meanwhile, facilities such as illumination, ventilation and the like and subsequent maintenance are not needed for expanding cultivation, and the transportation cost is reduced.
Description
Technical Field
The invention relates to the technical field of microalgae, in particular to a preservation method of filamentous algae.
Background
Filamentous algae is rich in unsaturated fatty acids, proteins, polysaccharides, inorganic pigments and various trace elements. Has wide application prospect in the industries of energy environment, medical food, breeding feed, cosmetics and the like. The industrial culture process of filamentous algae is generally divided into two parts of indoor seed expansion and outdoor culture, wherein the industrial culture is carried out by firstly expanding and culturing the filamentous algae indoors gradually and finally transferring the filamentous algae to an outdoor large-scale runway pool. However, the cultivation of filamentous algae is often restricted by factors such as season changes, microbial contamination, temperature and illumination changes, and the cultivation site does not have indoor algae-spreading conditions, so that the algae species cannot be supplied for outdoor large-scale cultivation. Therefore, high-activity algae needs to be transported to each production base in a long-distance transportation mode to meet production requirements.
The mode that traditional algae seed transportation adopted and will concentrate algae mud sealed, cold bright transport obtains the concentrated algae mud that the water content is greater than 98% promptly through modes such as centrifugation, and sealed cold bright transportation after preserving or directly transport the fresh algae seed culture solution of bulky to the destination. The two storage modes of filamentous algae have the following defects: the algae mud has high water content and water activity, and can cause mass propagation of microorganisms, so that the algae mud is rotten and deteriorated; the quality of the fresh algae seed culture solution is too large, and the fresh algae seed culture solution is difficult to store in a sealed mode, so that the transportation cost is high; in addition, intracellular substances are consumed by algae seeds due to respiration, and the activity of the algae seeds cannot be guaranteed after long-time transportation, so that the conditions of stagnant growth, increased pollution and the like occur in subsequent cultivation.
Disclosure of Invention
In view of this, the invention provides a preservation method of filamentous algae, and aims to solve the problems that the activity of cells is difficult to ensure and the transportation cost is high when the filamentous algae is transported and preserved in the prior art.
In one aspect, the present invention provides a preservation method of filamentous algae, comprising the following steps: a step of preparing filamentous algae mud, which is to reduce the water content of filamentous algae to a preset value to obtain algae mud; a pre-cooling step, namely adjusting the thickness of the algae mud prepared in the preparation step of the filamentous algae mud to a preset thickness, sealing the algae mud, and pre-cooling the algae mud for a first preset time at a first preset temperature; and a freezing and storing step, namely, placing the precooled algae mud prepared in the precooling step at a second preset temperature for a second preset time.
Further, in the above method for preserving filamentous algae, the process of preparing the algae slurry by filamentous algae is as follows: and performing at least one washing operation process and at least one water removal operation process on the filamentous algae to obtain algae mud, wherein the washing operation process and the water removal operation process are performed alternately.
Further, in the above method for preserving filamentous algae, when the washing operation and the dewatering operation are both at least twice, the filamentous algae are washed with a food grade preservative solution in the last washing operation, and the filamentous algae are washed with sterile water or a sterile culture solution in the washing operation before the last washing.
Further, in the above method for preserving filamentous algae, in the step of preparing filamentous algae mud, the water content of filamentous algae is reduced to a preset value, and the preset value is 60% to 70%.
Further, in the above method for preserving filamentous algae, the pre-cooling step further includes: the preset thickness is (1-1.5) cm, the first preset temperature is (2-5) DEG C, the first preset time is (4-12) h, the second preset temperature is (-1) DEG C, and the second preset time is (24-48) h.
Further, in the above method for preserving filamentous algae, the first preset temperature is 4 ℃; the second preset temperature is 0 ℃, and the second preset time is 36 h.
Further, in the preservation method of filamentous algae, the pre-cooling step further includes spraying an antifreezing agent on the surface of the algae mud after the algae mud is adjusted to a preset thickness, sealing the algae mud, cooling the algae mud at a third preset temperature for a third preset time, and pre-cooling the algae mud at the first preset temperature for a first preset time.
Further, in the preservation method of filamentous algae, in the step of freezing preservation, the third preset temperature is (-25 to-15) ℃, the third preset time is (10 to 30) min, and the first preset time is (0 to 12) h.
Further, in the preservation method of filamentous algae, in the step of freezing preservation, the third preset temperature is-20 ℃, and the third preset time is 20 min.
Further, in the above preservation method of filamentous algae, in the step of freezing preservation, the pre-cooled algae paste is preserved by using an ice bag at the second preset temperature.
Further, in the above method for preserving filamentous algae, the method for preserving filamentous algae further comprises: and a filamentous algae activity recovery step, namely standing the algae mud subjected to freezing storage in the freezing storage step for a preset recovery time at a preset illumination intensity and a preset recovery temperature for activity recovery.
Further, in the above preservation method of filamentous algae, the predetermined illumination intensity is (100-2(s) the preset recovery temperature is15-25) DEG C, and the preset recovery time is (20-36) h. According to the invention, the water content of the filamentous algae is reduced to a preset value, so that the water activity of the filamentous algae is reduced, the respiration consumption of algae cells is reduced in a short period of time, the intensity of life activities is reduced, the filamentous algae is precooled before being frozen and stored, the damage degree of the activity of the algae cells during the frozen storage is reduced, and the cell activity of the filamentous algae during the storage is increased.
Furthermore, the invention can inhibit the growth of most pollutants by a freezing preservation mode, can provide a large amount of high-activity algae seeds in a short period of time, and can be put into production immediately after being delivered to a destination, thereby improving the production efficiency.
Particularly, the invention sets continuous steps of precooling and cryopreservation, and ensures the activity of filamentous algae cells as much as possible while performing cryopreservation; meanwhile, the water content of the filamentous algae is gradually reduced by adopting the alternating process of at least one washing operation process and at least one water removal operation process, and compared with the traditional preservation method, the activity of the filamentous algae is not reduced due to the reduction of the water content.
Furthermore, compared with the liquid algae seed transportation in the prior art, the preservation method of filamentous algae provided by the invention has the advantages that the microalgae is transported by using the preservation method of filamentous algae, facilities such as illumination and aeration are not needed, and subsequent maintenance and propagation are not needed, so that the transportation cost is reduced.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a flow chart of a method for preserving filamentous algae according to an embodiment of the present invention;
FIG. 2 is a flowchart of a method for preserving filamentous algae according to an embodiment of the present invention.
Detailed Description
For a further understanding of the invention, preferred embodiments of the invention are described below in conjunction with the examples, but it should be understood that these descriptions are included merely to further illustrate the features and advantages of the invention and are not intended to limit the invention to the claims.
Please refer to fig. 1, which is a schematic flow chart of a filamentous algae preservation method according to the present invention, the method includes the following steps:
and step S1, reducing the water content of the filamentous algae to a preset value to obtain algae mud.
Specifically, any filamentous algae is selected for harvesting, preferably, filamentous algae with preset length of the filamentous algae can be selected to prepare the algae mud, for example, yellow filamentous algae with longer length of the filamentous algae is selected, so that the structure of the algae mud is looser, and the consistency of the internal and external temperatures of the algae mud in the transportation process is better maintained.
Then, the filamentous algae is subjected to at least one washing operation and at least one dewatering operation to obtain algae mud with a preset water content value of 60% -70%, wherein the washing operation process and the dewatering operation process are alternately performed, that is, the filamentous algae is subjected to one washing process and then subjected to one dewatering operation process. The steps can be repeated for a plurality of times to remove most protozoa, bacteria, miscellaneous algae and the like in the filamentous algae. When only one washing operation is performed, the filamentous algae can be washed by selecting sterile water, sterile culture solution or low-concentration food-grade preservative solution.
When the washing operation and the dewatering operation are at least twice, the filamentous algae are washed by food-grade preservative solution in the last washing process, the filamentous algae are washed by sterile water or sterile culture solution in the washing process before the last washing, and the propagation of microorganisms and other algae can be prevented after the filamentous algae are washed by low-concentration food-grade preservative solution (such as benzoic acid solution with the mass concentration of 0.1%) and dried.
In at least one dewatering operation, a method of not damaging the activity of algae cells such as screen filtration and spin-drying can be adopted. In specific implementation, after washing, a screen type filtering centrifuge can be used for filtering and centrifuging the filamentous algae so as to prevent damage to the activity of the filamentous algae cells in the process of removing water. By this step, the water activity of filamentous algae is adjusted to a value suitable for filamentous algae to maintain activity while reducing the water content of filamentous algae, and for example, the water activity may be adjusted to 0.98.
The water activity refers to the state of water in the food, i.e., the degree of binding (degree of release) of water to the food. The higher the water activity value, the lower the degree of binding of water to the food, the faster the microorganisms will multiply; the lower the water activity value, the higher the degree of binding of water to the food product, the more detrimental to microbial growth. Water activity is critical to microbial growth, enzyme activity and chemical reactions and is generally between 0 and 1, which is obtained by dividing the vapor pressure of food water by the vapor pressure of pure water (water activity of 1.0 for pure water).
Through the preparation step of the filamentous algae mud, the water content of filamentous algae is reduced to below 70%, the water activity is greatly reduced, ice crystals are prevented from being formed outside cells during freezing storage, and the cell rupture and the cell activity reduction caused by damage to cell membranes and cell walls of the algae are avoided; meanwhile, the water in the cells is reserved, and the activity of the substances in the cells is not influenced.
Step S2, adjusting the thickness of the algae paste prepared in the above step to a predetermined thickness, sealing, and pre-cooling at a first predetermined temperature for a first predetermined time.
Specifically, the dried algae mud can be adjusted to a preset thickness and sealed, and then directly placed in a first preset temperature environment for precooling for a first preset time. Wherein the preset thickness can be (1-1.5) cm, the first preset temperature can be (2-5) DEG C, and the preferable temperature is 4 ℃; the first preset time can be (4-12) h.
In specific implementation, when the thickness of the algae mud is larger than 1.5cm, the heat exchange speed is slow, the temperature in the center of the algae mud cannot be quickly reduced to 4 ℃, so that the microbial propagation and the respiration of algae cells are accelerated, and finally the activity of filamentous algae cells is reduced, therefore, the algae mud spun in the step S1 can be shaken to be fluffy, and one or more algae mud blocks with the preset thickness of (1-1.5) cm are arranged to be beneficial to the diffusion of the heat inside and outside the algae mud, so that the temperature inside and outside the algae mud keeps constant.
In order to shorten the first preset time of the pre-cooling step and reduce respiration consumption and microorganism propagation, in an embodiment of the present embodiment, the pre-cooling step may further include: after the algae mud is adjusted to the preset thickness, an antifreezing agent is sprayed on the surface of the algae mud, the algae mud is sealed, and after the algae mud is cooled for a third preset time at a third preset temperature, the algae mud is precooled for a first preset time at the first preset temperature. Wherein the preset thickness can be (1-1.5) cm, the third preset temperature can be (-25 to-15) DEG C, and the preferable temperature is-20 ℃; the third preset time can be (10-30) min, and is preferably 20 min; at this time, the first preset time may be (0-12) h. Specifically, adjusting filamentous algae into algae mud block with preset thickness, spraying antifreeze (such as dimethyl sulfoxide solution) on the surface of algae mud block, sealing with sealing bag, rapidly cooling at-20 deg.C for 20min, and pre-cooling at 4 deg.C for 4-12 h.
In another embodiment of this embodiment, before the pre-cooling step, the filamentous algae is washed with an anti-freezing agent, and after washing, the filamentous algae is dewatered to obtain a algal slurry, and the algal slurry is adjusted to a algal slurry block with a predetermined thickness, and then left to cool at a third predetermined temperature for a third predetermined time. Specifically, after the filamentous algae is washed for the last time, the filamentous algae is washed by an antifreezing agent (such as dimethyl sulfoxide solution), water in the filamentous algae is removed by a drying operation after the filamentous algae is washed, algae mud is obtained, the algae mud is adjusted to be algae mud blocks with the thickness of (1-1.5) cm and sealed by a sealing bag, the algae mud blocks are rapidly cooled for 20min at the temperature of minus 20 ℃, and then the algae mud blocks are transferred to the environment with the temperature of 4 ℃ for precooling (0-12) h.
Through the pre-cooling step, the filamentous algae cells can gradually adapt to the low-temperature environment, and the probability that the filamentous algae cells are damaged due to direct contact with low temperature is reduced.
And step S3, placing the precooled algae mud prepared in the precooling step at a second preset temperature for a second preset time.
Specifically, at the second preset temperature, the precooled algae mud can be preserved by using an ice bag. In specific implementation, an ice bag can be clamped between the precooled algae mud blocks with the thickness of (1-1.5) cm, namely: a bag of ice bags are stacked on each algae mud block, meanwhile, a layer of antifreeze (such as dimethyl sulfoxide solution) is sprayed on the surface layer of each algae mud block to prevent the activity of the algae mud on the surface layer which directly contacts the ice bags from being damaged, and then, after each algae mud block is sealed respectively, the algae mud blocks are placed in a second preset temperature environment for freezing storage, for example, the algae mud blocks can be placed in a foam box at 0 ℃ for sealing storage. The second preset temperature can be (-1) DEG C, and is preferably 0 ℃; the second preset time may be (24-48) h, preferably 36 h.
Through the step of freezing and preserving, the growth and the propagation of most microorganisms can be inhibited, the enzyme activity in algae cells is reduced, the respiration of the cells is reduced, the self consumption of substances in the cells is reduced, the cells can keep higher activity for a longer time, and the subsequent growth is not influenced after the cells are inoculated and cultured again.
In the embodiment, the water content of the filamentous algae is reduced to a preset value, so that the water activity of the filamentous algae is reduced, the respiratory consumption of the algae cells is reduced in a short period of time, the intensity of life activities is reduced, the filamentous algae is precooled before being frozen and stored, the damage degree of the activity of the algae cells during the frozen storage is reduced, and the cell activity of the filamentous algae during the storage is increased; and the growth of most pollutants can be inhibited, a large amount of high-activity algae seeds can be provided in a short period of time, and the algae seeds can be immediately put into production after being transported to a destination, so that the production efficiency is improved. Meanwhile, compared with the liquid algae seed transportation in the prior art, facilities such as illumination, ventilation and the like and subsequent maintenance are not needed for propagation, and the transportation cost is reduced.
Referring to fig. 2, in the above embodiments, the method for preserving filamentous algae may further include: a filamentous algae activity recovery step S4, in which the algae mud frozen and stored in the freezing and storing step is placed under a preset illumination intensity and a preset recovery temperature for a preset recovery time to recover the activity.
Specifically, after the algae seeds are transported to the destination, the algae seeds are cultured at a temperature lower than 15 ℃ or higher than 25 DEG CThe silk is easy to break and turn yellow, the speed of recovering the activity is slow or the survival rate of the algae is low, so the preset recovery temperature for recovering the activity of the algae mud can be (15-25) DEG C, and is preferably 20 ℃. In specific implementation, the filamentous algae after being frozen and stored can be transferred to a preset illumination intensity, for example, the activity can be recovered under the weak light condition. Wherein the predetermined illumination intensity is (100-150) μmol/m2S, preferably 130. mu. mol/m2S; the preset recovery time can be (20-36) h, and is preferably 24 h.
After the step of restoring the activity of the filamentous algae, the activity of the algae mud is further improved, the quantity of the conveyed high-activity algae is greatly increased, the algae mud is better used for outdoor normal culture, and the production efficiency is further improved.
The method for preserving filamentous algae will be described below with reference to specific examples.
Example 1
In the embodiment, the method comprises the steps of selecting and harvesting the chrysophyceae, performing microscopic examination after the chrysophyceae is harvested, washing with sterile water firstly when pollutants exist, and performing microscopic examination after washing without the pollutants; centrifuging and spin-drying at 1200 rpm by using a screen type filtering centrifuge to obtain algae mud with water content of 60%;
shaking and scattering the algae mud to be fluffy, putting the algae mud into algae mud blocks with the thickness of 1cm, sealing the algae mud blocks by using a sealing bag, and pre-cooling the algae mud blocks for 12 hours at the temperature of 4 ℃;
separating the algae mud blocks and the ice bag in the sealed bag at a ratio of 1:1, placing in a foam box at 0 ℃, sealing the foam box with an adhesive tape, and taking out after placing for 36 h; finally placing the mixture at the temperature of 25 ℃ and the illumination intensity of 130 mu mol/m2And (5) after 24 hours of recovery in the environment of/s, carrying out normal culture.
Example 2
In the embodiment, firstly, the chrysophyceae is selected for harvesting, after the chrysophyceae is harvested, the chrysophyceae is sequentially washed by sterile water and centrifugally dried at the rotating speed of 1400 revolutions per minute by a screen type filtering centrifuge, and the washing and drying operation is repeatedly carried out for 3 times to obtain alga mud with the water content of 65%;
shaking and scattering the algae mud to be fluffy, putting into algae mud blocks with the thickness of 1.2cm, spraying 30% dimethyl sulfoxide solution on the surfaces of the algae mud blocks for spraying, sealing the algae mud blocks by using a sealing bag, placing the sealed algae mud blocks in an environment at the temperature of minus 20 ℃ for rapid cooling for 20min, and transferring to an environment at the temperature of 2 ℃ for pre-cooling for 5 h;
placing the algae mud blocks and the ice bag in the sealed bag into a foam box at 0 ℃ at a ratio of 1:1 at an interlayer, sealing the foam box with an adhesive tape, and taking out after placing for 36 h; finally placing the mixture at the temperature of 25 ℃ and the illumination intensity of 100 mu mol/m2And (5) after 24 hours of recovery in the environment of/s, carrying out normal culture.
Example 3
In the embodiment, firstly, selecting and harvesting the chrysophyceae, washing the chrysophyceae with sterile water, a preservative solution and an antifreeze solution in sequence after harvesting, centrifugally spin-drying the chrysophyceae at the rotating speed of 1300 revolutions per minute by a screen type filtering centrifuge, and repeatedly carrying out washing and spin-drying operations for 4 times, wherein the last washing is carried out by using the antifreeze to obtain the algae mud with the water content of 70%;
shaking and scattering the algae mud to be fluffy, placing into algae mud blocks with the thickness of 1.5cm, sealing the algae mud blocks by using a sealing bag, and placing in an environment at the temperature of-15 ℃ for rapidly cooling for 30 min;
placing the algae mud blocks and the ice bag in the sealed bag into a foam box at the temperature of-1 ℃ at a ratio of 1:1 at an interlayer, sealing the foam box by using an adhesive tape, and taking out after placing for 24 hours; finally placing the mixture at the temperature of 20 ℃ and the illumination intensity of 120 mu mol/m2And (5) after the environment per second is recovered for 20 hours, carrying out normal culture.
Example 4
In the embodiment, firstly, the yellow silk algae is selected for harvesting, after the yellow silk algae is harvested, the yellow silk algae is sequentially washed by sterile water and centrifugally dried by a screen type filtering centrifuge at the rotating speed of 1500 revolutions per minute, and the washing and drying operation is repeatedly carried out for 6 times to obtain algae mud with the water content of 60 percent;
then shaking and scattering the algae mud to be fluffy, putting into algae mud blocks with the thickness of 1cm, spraying 30% dimethyl sulfoxide solution on the surfaces of the algae mud blocks for spraying, sealing the algae mud blocks by using a sealing bag, placing the sealed algae mud blocks in an environment at the temperature of minus 25 ℃, rapidly cooling for 10 min, and transferring to an environment at the temperature of 4 ℃ for precooling for 4 h;
placing the algae mud blocks and the ice bag in the sealing bag at a ratio of 1:1 at an interlayer, sealing the sealing bag, placing in a foam box at 1 ℃, sealing the foam box with an adhesive tape, placing for 48h, and taking out; finally placing the mixture at the temperature of 15 ℃ and the illumination intensity of 150 mu mol/m2And (5) after 36 hours of recovery in the environment of/s, carrying out normal culture.
Comparative example
Selecting and collecting the chrysophyceae, centrifuging to obtain concentrated algae mud, adding a small amount of glycerol with the mass concentration of 30% into the algae mud, placing the algae mud into a freshness protection package, sealing, storing for 48h in an environment at the temperature of minus 30 ℃, and then carrying out normal culture.
The final algae solutions obtained in the above examples 1 to 4 and comparative example were placed in centrifuge tubes of 15m l, and after being placed in a dark environment for 20 minutes, the chlorophyll fluorescence values of the algae solutions were measured by using a portable chlorophyll fluorescence detector, and the algae solutions were continuously cultured for 3 days, and the average yield of each algae solution was observed, and the measured parameter values were compared with the prior art fresh algae species without long-distance transportation, and the results are as follows:
it can be seen that the chlorophyll fluorescence value and the average yield of the filamentous algae liquid preserved by the filamentous algae preservation method provided by the invention are equivalent to those of fresh algae species, even superior to those of normal algae species, and compared with the filamentous algae preservation method in the prior art, the filamentous algae preserved by the preservation method provided by the invention has higher activity and can better meet the requirement of large-scale production.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (6)
1. A preservation method of filamentous algae is characterized by comprising the following steps:
the preparation method comprises the steps of preparing filamentous algae mud, namely performing at least one washing operation process and at least one dewatering operation process on filamentous algae, wherein the washing operation process and the dewatering operation process are alternately performed to reduce the water content of the filamentous algae to 60% -70% to obtain the algae mud; when the washing operation process and the dewatering operation process are at least twice, washing the filamentous algae with a food-grade preservative solution in the last washing operation process, and washing the filamentous algae with sterile water or a sterile culture solution in the washing operation process before the last washing;
a precooling step, namely adjusting the algae mud prepared in the preparation step of the filamentous algae mud to a preset thickness, spraying an antifreezing agent on the surface of the algae mud, sealing the algae mud, cooling the algae mud for a third preset time of 10-30 min at a third preset temperature of-25 to-15 ℃, and precooling the algae mud for a first preset time at a first preset temperature; the preset thickness is 1-1.5 cm, the first preset temperature is 2-5 ℃, and the first preset time is 4-12 hours;
a step of freezing and storing, namely placing the precooled algae mud prepared in the step of precooling at a second preset temperature for a second preset time; the second preset temperature is-1 ℃, and the second preset time is 24-48 h.
2. The method for preserving filamentous algae according to claim 1,
the first preset temperature is 4 ℃; the second preset temperature is 0 ℃, and the second preset time is 36 h.
3. The method for preserving filamentous algae according to claim 1, wherein the third predetermined temperature is-20 ℃ and the third predetermined time is 20 min.
4. The method for preserving filamentous algae according to claim 1, wherein in the step of freezing preservation, the pre-cooled algae paste is preserved with an ice bag at the second preset temperature.
5. The method for preserving filamentous algae according to claim 1, further comprising:
and a filamentous algae activity recovery step, namely standing the algae mud subjected to freezing storage in the freezing storage step for a preset recovery time at a preset illumination intensity and a preset recovery temperature for activity recovery.
6. The method for preserving filamentous algae as claimed in claim 5, wherein the predetermined illumination intensity is 100-2And/s, the recovery temperature is 15-25 ℃, and the preset recovery time is 20-36 h.
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