CN107384798A - The store method of filamentous algae - Google Patents
The store method of filamentous algae Download PDFInfo
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- CN107384798A CN107384798A CN201710586573.0A CN201710586573A CN107384798A CN 107384798 A CN107384798 A CN 107384798A CN 201710586573 A CN201710586573 A CN 201710586573A CN 107384798 A CN107384798 A CN 107384798A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention provides a kind of store method of filamentous algae, comprise the following steps：The water content of filamentous algae is down to preset value, obtains algal gel；Sealed after algal gel is adjusted to preset thickness, and the preset time of precooling first under the first preset temperature；The second preset time is preserved under algal gel after precooling is put to the second preset temperature.In the present invention, by the way that the water content of filamentous algae is reduced into preset value, make the reduction of its water activity, so that frustule short-term internal respiration consumption reduces, the intensity decreases of vital movement, the filamentous algae is subjected to precooling before freezen protective, the degree that algae cell activity sustains damage when reducing freezen protective, adds cytoactive when filamentous algae preserves；Further, it is possible to suppress the growth of most of pollutant, a large amount of high activity algae kinds can be provided in the short term, can immediately be put into production after being transported to destination, be improved production efficiency.Simultaneously, it is not necessary to which the facility such as illumination, ventilation and follow-up maintenance spread cultivation, and reduce cost of transportation.
Description
Technical field
The present invention relates to microalgae technical field, in particular to a kind of store method of filamentous algae.
Background technology
Filamentous algae is rich in unrighted acid, protein, polysaccharide, inorganic pigment and various trace elements.Energy environment,
The industries such as medicine food, breeding feed, cosmetics have broad application prospects.Filamentous algae industrialized aquaculture process is generally divided into
Indoor expanding species and outdoor breeding two large divisions, progressively expand culture by indoor expanding species first, are finally transferred to outdoor extensive
Raceway pond carries out industrialized aquaculture.But the culture of filamentous algae is often by seasonal law, microorganism pollution, temperature and light
Do not possess the restriction of the factor of indoor expansion algae condition according to change and cultivation site so that algae kind can not furnish good supplies to outdoor scale
Change cultivation.Therefore, it is necessary to by the form of long-distance transport, high activity algae kind is conveyed to each production base, meets production requirement.
Traditional algae kind transport is contained using the pattern that will concentrate algal gel sealing, cold fresh transport by modes such as centrifugations
Water is more than 98% concentration algal gel, is transported after sealing cold fresh preservation or directly transports the fresh algae kind nutrient solution of large volume
To destination.Following defect be present in the preserving type of both filamentous algaes：Algal gel water content and water activity are larger, can cause micro-
Biological amount reproduction, cause algal gel putrid and deteriorated；The quality of fresh algae kind nutrient solution is excessive, is not easy to seal up for safekeeping, causes cost of transportation
It is higher；Also, for algae kind because respiration consumes intracellular organic matter, transport for a long time can not ensure algae kind activity, cause subsequently to cultivate
There is growth arrest, pollute situations such as increasing.
The content of the invention
In consideration of it, the present invention proposes a kind of store method of filamentous algae, it is intended to solves filamentous algae in the prior art and is transporting
The activity of cell is difficult to ensure that and the problem of cost of transportation is higher during defeated preservation.
On one side, the present invention proposes a kind of store method of filamentous algae, comprises the following steps：The system of filamentous algae algal gel
Standby step, is down to preset value by the water content of filamentous algae, obtains algal gel；Precooling step, the preparation of above-mentioned filamentous algae algal gel is walked
Rapid obtained algal gel seals after adjusting to preset thickness, and the preset time of precooling first under the first preset temperature；Freezen protective
Step, the second preset time is preserved under the algal gel after precooling made from above-mentioned precooling step is put to the second preset temperature.
Further, in the store method of above-mentioned filamentous algae, the process that the algal gel is made in the filamentous algae is：To described
Filamentous algae carry out at least once washing operation and at least once water removal operation process to obtain algal gel, wherein, the washing
Operating process and the water removal operation process are alternately.
Further, in the store method of above-mentioned filamentous algae, when washing operation and water removal operation process are at least
When twice, the filamentous algae is washed with food-grade corrosion-resistant agent solution in last time washing operation, last
The filamentous algae is washed using sterilized water or aseptic culture fluid in washing operation before once washing.
Further, in the store method of above-mentioned filamentous algae, in the preparation process of the filamentous algae algal gel, by filamentous algae
Water content is down to preset value, and the preset value is 60%~70%.
Further, in the store method of above-mentioned filamentous algae, the precooling step also includes：The preset thickness for (1~
1.5) cm, first preset temperature are (2~5) DEG C, and first preset time is (4~12) h, second preset temperature
It is (24-48) h for (- 1~1) DEG C, second preset time.
Further, in the store method of above-mentioned filamentous algae, first preset temperature is 4 DEG C；Described second default temperature
Spend for 0 DEG C, second preset time is 36h.
Further, in the store method of above-mentioned filamentous algae, the precooling step also includes:Adjusted in the algal gel to pre-
If after thickness, antifreezing agent is sprayed on the algal gel surface, and by after algal gel sealing, the 3rd is cooled down under the 3rd preset temperature
After preset time, by the algal gel under first preset temperature preset time of precooling first.
Further, in the store method of above-mentioned filamentous algae, in the freezen protective step, the 3rd preset temperature is
(- 25~-15) DEG C, the 3rd preset time are (10~30) min, and first preset time is (0-12) h.
Further, in the store method of above-mentioned filamentous algae, in the freezen protective step, the 3rd preset temperature
For -20 DEG C, the 3rd preset time is 20min.
Further, in the store method of above-mentioned filamentous algae, in the freezen protective step, in second preset temperature
Under, the algal gel after precooling is preserved with ice bag.
Further, in the store method of above-mentioned filamentous algae, the store method of the filamentous algae also includes：Filamentous algae activity
Recovering step, by the algal gel after above-mentioned freezen protective step freezen protective in default intensity of illumination and default recovery temperature
It is lower to place default recovery time progress activation recovering.
Further, in the store method of above-mentioned filamentous algae, the default intensity of illumination is (100-150) μm ol/m2/ s,
The default recovery temperature is (15~25) DEG C, and the default recovery time is (20~36) h.In the present invention, by will be thread
The water content of algae is reduced to preset value, makes the reduction of its water activity, so that frustule short-term internal respiration consumption reduces, life
Activity intensity decreases, the filamentous algae is subjected to precooling before freezen protective, when reducing freezen protective algae cell activity by
To the degree of damage, cytoactive when filamentous algae preserves is added.
Further, the present invention can suppress the growth of most of pollutant by way of freezen protective, can be short
A large amount of high activity algae kinds are provided in period, can immediately be put into production after being transported to destination, improve production efficiency.
Especially, the present invention is by setting continuous precooling and freezen protective step, while freezen protective, as far as possible
The activity that ensure that thread frustule；Meanwhile by using washing operation at least once and water removal operation at least once
Alternation procedure, make the water content of filamentous algae gradually reduce, compared to traditional store method, filamentous algae not because moisture reduce and reduce
Activity.
Further, the store method of filamentous algae of the present invention uses this hair compared with liquid algae kind in the prior art is transported
Bright microalgae store method is transported, it is not necessary to set the facilities such as illumination, ventilation and follow-up maintenance to spread cultivation, reduce transport into
This.
Brief description of the drawings
By reading the detailed description of hereafter preferred embodiment, it is various other the advantages of and benefit it is common for this area
Technical staff will be clear understanding.Accompanying drawing is only used for showing the purpose of preferred embodiment, and is not considered as to the present invention
Limitation.And in whole accompanying drawing, identical part is denoted by the same reference numerals.In the accompanying drawings：
Fig. 1 is the flow chart of the store method of filamentous algae provided in an embodiment of the present invention；
Fig. 2 is the another flow chart of the store method of filamentous algae provided in an embodiment of the present invention.
Embodiment
In order to further appreciate that the present invention, the preferred embodiments of the invention are described with reference to embodiment, but
It is it should be appreciated that these descriptions are simply for further explanation the features and advantages of the present invention rather than to patent requirements of the present invention
Limitation.
Refer to shown in Fig. 1, it is the schematic flow sheet of the store method of the filamentous algae of the present invention, and this method includes following
Step：
Step S1, the water content of filamentous algae is down to preset value, obtains algal gel.
Specifically, choose that any one filamentous algae is thread to be harvested first, it is preferable that can choose with default algal filament
The filamentous algae of length prepares algal gel, such as chooses the longer Huang Sizao of trichome length so that algal gel structure is looser, is more beneficial for
Keep algal gel inside and outside temperature consistent in transportation.
Then, washing operation at least once is carried out to the filamentous algae and water removal operation is preset with obtaining water content at least once
Value can be 60%~70% algal gel, wherein, washing operation and water removal operation process are alternately, that is to say, that right
Filamentous algae carries out a water removal operation process after carrying out once washing process.Can so it circulate repeatedly, to remove in filamentous algae
Most of protozoan, bacterium and miscellaneous algae etc..When only carrying out once washing operating process, sterilized water, sterile training can be chosen
Nutrient solution or low concentration food-grade corrosion-resistant agent solution wash to filamentous algae.
When washing operation and water removal operation process are at least twice, food is used in last time washing process
Level antiseptic solution is washed to filamentous algae, and sterilized water or sterile is used in the washing process before last time is washed
Nutrient solution washs to filamentous algae, with low concentration food-grade corrosion-resistant agent solution (such as mass concentration be 0.1% benzoic acid it is molten
Liquid) filamentous algae is washed and dried after can prevent the breeding of microorganism and miscellaneous algae.
During water removal operation at least once, screen filtration and drying etc. can be used not to damage algae cell activity
The method of wound.When it is implemented, after washing, filamentous algae can be filtered, centrifuged using screen mesh type filtering centrifuge, with
Prevent the damage to caused by thread algae cell activity in moisture process is removed.By the step, filamentous algae water content is being reduced
While by the water activity of filamentous algae adjust to suitable filamentous algae keep activity value, such as water activity can be adjusted to
0.98。
It should be noted that water activity refers to state existing for moisture in food, i.e. moisture (dissociates with food combination degree
Degree).Water activity value is higher, and the combination degree of moisture and food is lower, and microbial reproduction is faster；Water activity value is got over
Low, the combination degree of moisture and food is higher, and it is more unfavorable that microorganism is grown.Water activity to microorganism grow, enzymatic activity and
Chemical reaction is very crucial, and typically between 0 to 1, it is divided by obtain (pure water its numerical value by food water vapor pressure and pure water vapor pressure
Water activity for 1.0).
By the preparation process of filamentous algae algal gel, the water content of filamentous algae is reduced to less than 70%, substantially reduced simultaneously
Water activity, prevent from forming ice crystal in outside when freezen protective, avoid frustule film and cell membrane are caused
Damage causes cell rupture, cytoactive to reduce；The moisture of intracellular is remained simultaneously, does not influence the activity of intracellular organic matter.
Step S2, sealed after algal gel made from the preparation process of above-mentioned filamentous algae algal gel is adjusted to preset thickness, and
The preset time of precooling first under first preset temperature.
Specifically, the algal gel of drying can be adjusted to preset thickness and sealing, is placed directly in the first preset temperature
The preset time of precooling first is carried out in environment.Wherein, preset thickness can be (1~1.5) cm, and the first preset temperature can be (2
~5) DEG C, preferably 4 DEG C；First preset time can be (4~12) h.
When it is implemented, because when the thickness of algal gel is more than 1.5cm, rate of heat exchange can be relatively slow, algal gel central interior
Temperature can not be quickly reduced to 4 DEG C, cause microbial reproduction and frustule respiration to be accelerated, and ultimately cause thread frustule and live
Property reduction, therefore, can by step S1 dry after algal gel shake it is fluffy, it is (1- to be put into one or more preset thickness
1.5) cm algal gel block is in favor of the diffusion of heat inside and outside algal gel so that algal gel inside and outside temperature keeps constant.
In order to shorten the first preset time of precooling step, respiration consumption and microbial reproduction are reduced, in the present embodiment
In a kind of embodiment, precooling step can also include：Adjust to preset thickness, sprayed on algal gel surface antifreeze in algal gel
Agent, and after algal gel is sealed, after cooling down the 3rd preset time under the 3rd preset temperature, by algal gel in the described first default temperature
The lower preset time of precooling first of degree.Wherein, preset thickness can be (1~1.5) cm, the 3rd preset temperature can be (- 25~-
15) DEG C, it is preferably -20 DEG C；3rd preset time can be (10~30) min, preferably 20min；Now, the first preset time
Can be (0-12) h.When it is implemented, after filamentous algae to be adjusted to the algal gel block of preset thickness, sprayed on algal gel block surface
Antifreezing agent (such as dimethyl sulphoxide solution) afterwards, sealed with hermetic bag and rapidly cool down 20min in the environment of being placed on -20 DEG C,
Progress precooling (4-12) h in 4 DEG C of environment is transferred to afterwards.
In the another embodiment of the present embodiment, proceeded as follows before precooling step:Washed for the last time
When washing filamentous algae, filamentous algae is washed with antifreezing agent, carrying out water removal operation to filamentous algae after having washed obtains algal gel, and algal gel is adjusted
Into after the algal gel block of preset thickness, the 3rd preset time of cooling under the 3rd preset temperature is placed on.When it is implemented, at last
After secondary washing filamentous algae, grasped after being washed, washed to filamentous algae with antifreezing agent (such as dimethyl sulphoxide solution) by drying
Make to obtain algal gel after the moisture in removing filamentous algae, algal gel is adjusted to the algal gel block that thickness is (1-1.5) cm and uses hermetic bag
After sealing, 20min is rapidly cooled down at -20 DEG C, algal gel block is transferred to progress precooling (0-12) h in 4 DEG C of environment again afterwards.
By precooling step, filamentous algae cell can be made progressively to adapt to low temperature environment, reduce thread frustule because directly connecing
The probability for touching low temperature and sustaining damage.
Step S3, second is preserved under the algal gel after precooling made from above-mentioned precooling step is put to the second preset temperature and is preset
Time.
Specifically, under the second preset temperature, the algal gel after precooling can be preserved with ice bag.When it is implemented,
Can it is above-mentioned it is each it is pre-cooled after thickness be (1-1.5) cm algal gel block between sandwiched ice bag, i.e.,：On each algal gel block
One bag of ice bag is stacked, meanwhile, it is straight to prevent that one layer of antifreezing agent (such as dimethyl sulphoxide solution) is sprayed on the top layer of each algal gel block
The activity for contacting the top layer algal gel of ice bag sustains damage, and after then each algal gel block is sealed respectively, it is default to be positioned over second
Freezen protective is carried out in temperature environment, such as can be placed in 0 DEG C of bubble chamber and be sealed.Second preset temperature can be with
For (- 1~1) DEG C, preferably 0 DEG C；Second preset time can be (24-48) h, preferably 36h.
By freezen protective step, the growth and breeding of most of microorganism can be suppressed, while reduce the enzyme in frustule
Activity, cellular respiration is reduced, reduce the consumption of intracellular matter itself so that cell can keep higher in a long time
Activity, after renewed vaccination cultivation, do not influence subsequent growth.
In the present embodiment, by the way that the water content of filamentous algae is reduced into preset value, make the reduction of its water activity so that frustule
Short-term internal respiration consumption reduces, and the intensity decreases of vital movement, the filamentous algae is carried out into precooling before freezen protective, reduces
The degree that algae cell activity sustains damage during freezen protective, add cytoactive when filamentous algae preserves；Further, it is possible to press down
The growth of most of pollutant is made, a large amount of high activity algae kinds can be provided in the short term, being transported to behind destination can be immediately
Put into production, improve production efficiency.Meanwhile compared with liquid algae kind in the prior art is transported, it is not necessary to which illumination, ventilation etc. are set
Apply and spread cultivation with follow-up maintenance, reduce cost of transportation.
Referring to Fig. 2, in the various embodiments described above, the store method of filamentous algae can also include：Filamentous algae rejuvenation step
S4, the algal gel after above-mentioned freezen protective step freezen protective is placed in advance under default intensity of illumination and default recovery temperature
If recovery time carries out activation recovering.
Specifically, because algae kind is transported to destination, when temperature is less than 15 DEG C or more than 25 DEG C, algal filament easily goes out
Now fracture, the phenomenon of its colour changed into yellow, the speed of activity recovery is relatively low compared with slow or algae kind survival rate, and therefore, algal gel is carried out
The default recovery temperature of activation recovering can be (15~25) DEG C, preferably 20 DEG C.Protected when it is implemented, freezing can will be passed through
Filamentous algae after depositing is transferred under default intensity of illumination, such as can carry out activation recovering under low light condition.Wherein, preset
Intensity of illumination is (100-150) μm ol/m2/ s, preferably 130 μm of ol/m2/s；Default recovery time can be (20~36) h, excellent
Elect 24h as.
After filamentous algae rejuvenation step, the activity of algal gel is further increased, also causes the high activity algae of conveying
Kind of quantity greatly increases, be preferably applied to it is outdoor normally cultivated, further increase production efficiency.
The store method of filamentous algae is illustrated below by specific embodiment.
Embodiment 1
In the present embodiment, yellow silk algae is chosen first to be harvested, and after yellow silk algae is harvested, carries out microscopy, there is pollutant,
First with sterile water washing, microscopy does not have pollutant after washing；Again with screen mesh type filtering centrifuge under 1200 revs/min of rotating speed
Centrifuge dripping, obtain the algal gel that water content is 60%；
Algal gel is shaken loose to soft fluffy state, the thick algal gel blocks of 1cm is put into, is sealed with hermetic bag, be placed into 4 DEG C of environment
Lower progress precooling 12 hours；
Algal gel block in hermetic bag and ice bag are pressed 1:1 ratio interlayer, is positioned in 0 DEG C of bubble chamber, blend compounds band
Bubble chamber is sealed, taken out after placing 36h；Finally be positioned over temperature be 25 DEG C, intensity of illumination be 130 μm of ol/m2/ s's
After recovering 24h in environment, normally cultivated.
Embodiment 2
In the present embodiment, yellow silk algae is chosen first to be harvested, by yellow silk algae harvest after, successively through sterile water washing,
Through screen mesh type filtering centrifuge, centrifuge dripping, washing and spin-drying operation are repeated 3 times, obtained under 1400 revs/min of rotating speed
To the algal gel that water content is 65%；
Algal gel is shaken loose to soft fluffy state, is put into the thick algal gel blocks of 1.2cm, sprays 30% diformazan on the surface of algal gel block
Base sulfoxide solution is sprayed, and after with hermetic bag, algal gel block is sealed, is placed into after rapidly being cooled down 20min under -20 DEG C of environment, is turned
Move to and precooling 5h is carried out under 2 DEG C of environment；
Algal gel block in above-mentioned hermetic bag and ice bag are pressed 1:1 ratio interlayer is positioned in 0 DEG C of bubble chamber, blend compounds
Band seals bubble chamber, is taken out after placing 36h；Temperature is finally positioned over as 25 DEG C, intensity of illumination is 100 μm of ol/m2/s
Environment in recover 24h after, normally cultivated.
Embodiment 3
In the present embodiment, choose yellow silk algae first to be harvested, after yellow silk algae is harvested, successively through sterilized water, anti-corrosion
Agent solution and antifreeze agent solution washing, through screen mesh type filtering centrifuge under 1300 revs/min of rotating speed centrifuge dripping, washing and
Spin-drying operation is repeated 4 times, wherein, last time is washed when washing using antifreezing agent, and it is 70% to obtain water content
Algal gel；
Then algal gel is shaken loose to soft fluffy state, is put into the thick algal gel block hermetic bags of 1.5cm by after the sealing of algal gel block, puts
Put and rapidly cool down 30min under -15 DEG C of environment；
Algal gel block in above-mentioned hermetic bag and ice bag are pressed 1:1 ratio interlayer is positioned in -1 DEG C of bubble chamber, is used in combination
Adhesive tape seals bubble chamber, is taken out after placing 24h；Temperature is finally positioned over as 20 DEG C, intensity of illumination is 120 μm of ol/m2/
After recovering 20h in s environment, normally cultivated.
Embodiment 4
In the present embodiment, yellow silk algae is chosen first to be harvested, by yellow silk algae harvest after, successively through sterile water washing,
Through screen mesh type filtering centrifuge, centrifuge dripping, washing and spin-drying operation are repeated 6 times, obtained under 1500 revs/min of rotating speed
To the algal gel that water content is 60%；
Then algal gel is shaken loose to soft fluffy state, is put into the thick algal gel blocks of 1cm, spray the two of 30% on the surface of algal gel block
Methyl sulfoxide solution is sprayed, and after algal gel block is sealed with hermetic bag, is placed under -25 DEG C of environment and is rapidly cooled down 10mi n
Afterwards, it is transferred under 4 DEG C of environment and carries out precooling 4h；
And the algal gel block in hermetic bag and ice bag are pressed 1:After 1 ratio interlayer places, hermetic bag is sealed, is positioned over
In 1 DEG C of bubble chamber, finally bubble chamber is sealed with adhesive tape, taken out after placing 48h；Temperature is finally positioned over as 15 DEG C,
Intensity of illumination is 150 μm of ol/m2After recovering 36h in/s environment, normally cultivated.
Comparative example
Choose after yellow silk algae harvested, obtain concentrating algal gel after centrifuging it, a small amount of matter is added into the algal gel
The glycerine that concentration is 30% is measured, is put into after freshness protection package sealing and after preservation 48h, is normally cultivated in -30 DEG C of environment.
The algae solution that will finally be obtained in above-described embodiment 1 to 4 and comparative example, it is respectively placed in 15m l centrifuge tube, so
Afterwards after dark situation is placed 20 minutes, respective Fluorometer is detected using portable chlorophyll fluorescence detector, and
Continuous cultivation 3 days, observe the average product of each algae solution, by the parameter value obtained after testing with it is of the prior art without long-distance fortune
Defeated fresh algae kind is compared, as a result such as following table:
As can be seen that the Fluorometer of the filamentous algae algae solution preserved by filamentous algae store method provided by the invention
It is suitable with the Fluorometer and average product of fresh algae kind with average product, even better than normal algae kind, with prior art
In filamentous algae store method compare, by store method provided by the invention preserve filamentous algae activity it is higher, can be preferably
Meet large-scale production.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to comprising including these changes and modification.
Claims (12)
1. a kind of store method of filamentous algae, it is characterised in that comprise the following steps：
The preparation process of filamentous algae algal gel, the water content of filamentous algae is down to preset value, obtains algal gel；
Precooling step, sealed after algal gel made from the preparation process of above-mentioned filamentous algae algal gel is adjusted to preset thickness, and
The preset time of precooling first under one preset temperature；
Freezen protective step, is preserved under the algal gel after precooling made from above-mentioned precooling step is put to the second preset temperature
Two preset times.
2. the store method of filamentous algae according to claim 1, it is characterised in that the algal gel is made in the filamentous algae
Process is：To the filamentous algae carry out at least once washing operation and at least once water removal operation process to obtain algal gel,
Wherein, the washing operation and the water removal operation process be alternately.
3. the store method of filamentous algae according to claim 2, it is characterised in that when washing operation and water removal operation
When process is at least twice, the filamentous algae is carried out with food-grade corrosion-resistant agent solution in last time washing operation
Washing, the filamentous algae is entered using sterilized water or aseptic culture fluid in the washing operation before last time is washed
Row washing.
4. the store method of filamentous algae according to claim 1, it is characterised in that
In the preparation process of the filamentous algae algal gel, the water content of filamentous algae is down to preset value, the preset value is 60%~
70%.
5. the store method of filamentous algae according to claim 1, it is characterised in that
The preset thickness is (1~1.5) cm, and first preset temperature is (2~5) DEG C, and first preset time is (4
~12) h, second preset temperature is (- 1~1) DEG C, second preset time is (24-48) h.
6. the store method of filamentous algae according to claim 5, it is characterised in that
First preset temperature is 4 DEG C；Second preset temperature is 0 DEG C, and second preset time is 36h.
7. the store method of filamentous algae according to claim 2, it is characterised in that the precooling step also includes:
Adjusted in the algal gel to preset thickness, the algal gel surface spray antifreezing agent, and by the algal gel seal after,
After cooling down the 3rd preset time under 3rd preset temperature, by the algal gel, precooling first is preset under first preset temperature
Time.
8. the store method of filamentous algae according to claim 7, it is characterised in that
3rd preset temperature is (- 25~-15) DEG C, and the 3rd preset time is (10~30) min, and described first is default
Time is (0-12) h.
9. the store method of filamentous algae according to claim 8, it is characterised in that the 3rd preset temperature is -20 DEG C,
3rd preset time is 20min.
10. the store method of filamentous algae according to claim 1, it is characterised in that in the freezen protective step, in institute
State under the second preset temperature, the algal gel after precooling is preserved with ice bag.
11. the store method of filamentous algae according to claim 1, it is characterised in that the store method of the filamentous algae is also
Including：
Filamentous algae rejuvenation step is strong in default illumination by the algal gel after above-mentioned freezen protective step freezen protective
Default recovery time progress activation recovering is placed under degree and default recovery temperature.
12. the store method of filamentous algae according to claim 11, it is characterised in that the default intensity of illumination is
(100-150)μmol/m2/ s, the recovery temperature are (15~25) DEG C, and the default recovery time is (20~36) h.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101524110A (en) * | 2009-03-27 | 2009-09-09 | 张跃群 | Production process for marine microalgae living body freezing paste |
| CN102311919A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Microalgae species preserving method |
-
2017
- 2017-07-18 CN CN201710586573.0A patent/CN107384798B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101524110A (en) * | 2009-03-27 | 2009-09-09 | 张跃群 | Production process for marine microalgae living body freezing paste |
| CN102311919A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Microalgae species preserving method |
Non-Patent Citations (3)
| Title |
|---|
| JOHN G. DAY ET AL.: "In vitro culture and conservation of microalgae: Applications for aquaculture, biotechnology and environmental research", 《IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT》 * |
| 张文慧等: "海洋微藻保存方法的研究进展", 《天津农学院学报》 * |
| 林小园等: "海洋微藻的玻璃化冻存技术研究进展", 《江苏农业科学》 * |
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