CN107377614A - Screening technique and application for the nutritive salt of pollutant microbial augmentation reparation - Google Patents
Screening technique and application for the nutritive salt of pollutant microbial augmentation reparation Download PDFInfo
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- CN107377614A CN107377614A CN201710590579.5A CN201710590579A CN107377614A CN 107377614 A CN107377614 A CN 107377614A CN 201710590579 A CN201710590579 A CN 201710590579A CN 107377614 A CN107377614 A CN 107377614A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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Abstract
The invention discloses a kind of screening technique of the nutritive salt for pollutant microbial augmentation reparation, comprise the following steps：1）Culture medium is separately added into the fluid sample containing pollutant, then observes the growing state of flora, carries out baseline screening；2）Into the fluid sample containing pollutant, different ratio, different types of nutritive salt progress Shaking culture are added respectively, the quantity of microbe colony is tested by plate count and ATP bioluminescences detector；3）Detection adds the concentration of pollutant in the fluid sample containing pollutant after nutritive salt culture, the pollutant repairing effect of nutritive salt enhancement microbiological is evaluated according to result above, so as to draw optimal nutritive salt formula.
Description
Technical field
The invention belongs to microbial augmentation recovery technique in field of environment engineering technology, and in particular to one kind is used for pollutant
The screening technique of the nutritive salt of microbial augmentation reparation and the nutritive salt filtered out by the screening technique, more particularly on six
The screening technique of the nutritive salt of valency chromium microbial augmentation reparation.
Background technology
In recent years, with the high speed development of national economy, the process problem of pollutant is that current mankind society closes extensively
One problem of note.
" five poisonous creatures: scorpion, viper, centipede, house lizard, toad " in heavy metal, i.e. chromium, cadmium, mercury, arsenic and lead.Wherein water-soluble and maximum migration is Cr VI.
Cr VI is typically produced by chromium salt production producer, typically all preservation in have calcium be calcined caused by chromium slag, and be present in the later stage
Because eluviation causes in chromium slag contaminated soil and groundwater around it.Cr VI is mainly taken the photograph by skin contact, orally
Enter and breathe the route of exposure such as suction and very big health threat is caused to human body, while the migration of Cr VI is very big, to surrounding
Soil and groundwater causes very big pollution.
The improvement recovery technique of Cr VI is broadly divided into source and non-goes the major class of source two at present.Go source mainly include elution,
Dump leaching, electro reclamation etc.；It is non-to go to source mainly to include electronation stabilization, solidification and stabilization, wet method detoxifcation, dry detoxified etc..
Elution, dump leaching method
Elution and dump leaching mainly carry out leaching or immersion using ferrous iron solution, sulfuric acid or water to chromium-polluted soil and chromium slag,
Cr VI separates source.
Advantage is Cr VI can really to be separated, and reaches the purpose in source；Shortcoming is leacheate or heap leaching solution
Need to establish special sewage treatment facility and be disposed, and price is costly.
Chemical reduction method
Wherein chemical reduction method mainly by add ferrous sulfate, sodium pyrosulfite, sodium dithionite, iron powder, vulcanized sodium,
The reducing substanceses such as calcium polysulfide reach the purpose for being reduced into trivalent chromium as electron donor to Cr VI.With ferrous ion and six
Valency chromium reaction equation illustrates as an example：
By taking 1000g soil or chromium slag as an example, the concentration of Cr VI is 9970mg/kg, so the quality of Cr VI is 9970mg, i.e.,
0.19173mol.Desired value is 64mg/kg, corresponding 0.00123mol, and needs the Cr to 0.1905mol6+Carry out reduction treatment,
Needed to add 0.5715mol Fe according to above-mentioned reaction equation2+As reducing agent.
Corresponding ferrous sulfate addition is 25%, and ferrous sulfate addition is 250g, effective content 87%, i.e.,
217.5g, molecular weight 278g/mol, so being 0.7824mol, medicament addition safety coefficient is 1.3690.The medicament added more
On the one hand it is used to react with other oxidation state species that can consume ferrous iron present in waste residue, is on the other hand prevented for the later stage
Cr3+Rebound into Cr6+And provide a reducing environment.
Due to the addition of substantial amounts of ferrous sulfate or other materials, cause the sulfate in the place after administering exceeded, soil
Secondary salinization, it is hardened the problems such as.
Solidification and stabilization
Solidification and stabilization method is based on the basis of electronation, then adds some curing agent, such as cement, lime, fine coal
The silicate materials such as ash, hydraulic material can be generated by hydration reaction, make trivalent chromium generation chromium hydroxide precipitation or by three
Valency chromium is wrapped in lattice.
Problem is that compatibilization is larger, and the pH value for ultimately producing thing is typically in alkaline range（PH=10 or so）；Advantage is energy
It is enough to complete disposal task in the shorter time.
Wet method detoxifcation
Wet method detoxifcation is disposed generally be directed to the chromium slag or chromium-polluted soil of high concentration.
Pre-acidified is carried out using sulfuric acid to strengthen the reduction effect of Cr VI.Chromium slag is through being finely ground to 200 mesh first（0.075
Micron）Particle diameter, then add sulfuric acid, after copperas solution and water are sufficiently stirred, piece alkali regulation pH=9.5 added, in alkalescence
Under the conditions of trivalent chromium precipitated in the form of chromium hydroxide, then obtain mud cake through plate compression, press filtration water pump is sent to sewage disposal
Station is uniformly processed.
Advantage is thoroughly hexavalent chrome reduction into trivalent chromium and to be precipitated, but device hardware input early stage is very
Greatly, energy consumption is also very high.
It is dry detoxified
It is dry detoxified mainly to use rotary kiln or shaft kiln as calciner, at high temperature will using reducing substanceses such as anthracites
Hexavalent chrome reduction in chromium slag is trivalent chromium and the technical process for fixing it at normal temperatures.
Advantage is thoroughly hexavalent chrome reduction into trivalent chromium and to be precipitated, but device hardware input early stage is very
Greatly, energy consumption is also very high.
In summary, although using elution, dump leaching, wet method detoxifcation or it is dry detoxified processing ratio more thoroughly, high energy consumption,
Cost is high and needs substantial amounts of chemical reagent.
Using although there is very big advantage in electronation stabilization or solidification and stabilization cost, due to going
Source, the trivalent chromium of reduction are easy to bounce-back brightness reversion and are oxidized to Cr VI again, and the long-time stability of medicament cannot be guaranteed.
In order to meet Cr VI Practical Project demand, it is ensured that in quality guarantee period and long-time stability, utilizing chemical method
Microbial augmentation reparation is added while reparation.
Microorganism remediation method with environment-friendly, low cost and other advantages by many scholars due to being paid close attention to.Microorganism
It is mainly through domestication using the indigenous microorganism or exogenous artificial addition stimulated in reinforced soil to repair chromium-polluted soil
High-effective microorganism, reacted, poisonous, moveable Cr (VI) is reduced to low toxicity, not easily shifted by micro-reduction
Cr (III), reach the purpose for administering soil pollution of chromium.
There is strict management and control regulation in exogenous microbial country, and needs early stage and carry out substantial amounts of high flux screening, and
And there is competitive relation in the exogenous flora for needing to add with original indigenous microorganism.Preferentially using indigenous microorganism to Cr (VI)
Reduction and detoxication is carried out, strengthens repairing effect, has in terms of environmental safety, environmental suitability, population harmony and application cost
There is the incomparable superiority of other strange land strains.So find a kind of proper business of method change for stimulating and strengthening indigenous microorganism
It is anxious.
The content of the invention
Strengthen problem to solve the stimulation of indigenous microorganism, the invention provides one kind to be used for Cr VI microbial augmentation
The rapid screening method of the nutritive salt of reparation.By quickly screening high efficiency nutritious salt, be added to processing after hexavalent chromium polluted soil
Earth, waste residue etc. carry out reinforcing reparation, strengthen its long-time stability.
The present invention provides a kind of screening technique of nutritive salt for pollutant microbial augmentation reparation, including following step
Suddenly：
1）Culture medium is separately added into the fluid sample containing pollutant, then observes the growing state of flora, carries out baseline sieve
Choosing；（To determine that the concentration of pollutant is appropriate for microorganism remediation, the terminal for antecedent chemical reparation provides guidance；Use simultaneously
To determine that there is considerable microorganism species quantity in fluid sample）
2）Into the fluid sample containing pollutant, different ratio, different types of nutritive salt progress Shaking culture are added respectively, is led to
Plate count and ATP bioluminescences detector are crossed to test the quantity of microbe colony；
3）Detection adds the concentration of Cr VI in the fluid sample containing pollutant after nutritive salt culture, according to result above to battalion
The pollutant repairing effect for supporting salt enhancement microbiological is evaluated, so as to draw optimal nutritive salt formula.
Preferably, the fluid sample containing pollutant is the leachate of the solid sample containing pollutant, contaminants
Underground water and contaminants surface water one or more kinds of any combination.
Preferably, the pollutant is hexavalent chromium pollutant, and the fluid sample containing pollutant is hexavalent chromium polluted solid
The leachate of pollutant, hexavalent chromium polluted underground water and hexavalent chromium polluted surface water it is one or more kinds of any
Combination.Preferably, the culture medium is THIOGLYCOLLIC ACID salt broth.
Preferably, the preparation method of the leachate of the hexavalent chromium polluted solid pollutant is as follows：Hexavalent chromium concentration
In below 100mg/kg pollution of chromium solid pollutant or after the stable pretreatment of electronation, hexavalent chromium concentration is reduced to
The solid pollutant of below 100mg/kg pollution of chromium, particle diameter are milled down to below 1cm, add the steaming of deionized water or comparable purity
Distilled water, immersion, takes supernatant to produce.
Preferably, the liquid-solid ratio that prepared by leachate is 5:1—10:1.
Preferably, the immersion incorporation time in leachate preparation process is 6-8 hours, and is manually stirred every 2 hours
Mix 1 minute, time of repose is 1-2 hours.
Preferably, the nutritive salt be compound organic fertilizer, methanol, urea and glucose it is one or more kinds of any
Combination.
Preferably, the Shaking culture time is 5-10 days.
Preferably, the culture medium is nutrient broth medium, TTC nutrient agars, agar powder culture medium, sulphur second
One or more kinds of any combination of alcohol hydrochlorate broth.
Preferably, the nutritive salt is humic acid, prosparol, compound organic fertilizer, urea, corncob, beef jelly, first
Alcohol, glucose, one or more kinds of any combination of brewex's grains.
The present invention also provides a kind of is used for Cr VI microbial augmentation reparation using what above-mentioned screening technique screened to obtain
Nutritive salt, the nutritive salt be humic acid, prosparol, compound organic fertilizer, urea, corncob, beef jelly, methanol, Portugal
Grape are sugared, one or more kinds of any combination of brewex's grains, and the carbon-nitrogen mass ratio in the nutritive salt is C:N =10:1~10.
Preferably, carbon-nitrogen mass ratio is C in the nutritive salt:N=10:Carbon nitrogen phosphorus mass ratio is C in 5, or the nutritive salt:N:P=
10:5:1—10:5:2。
Nutritive salt for Cr VI microbial augmentation reparation is preferably glucose and urea or compound organic fertilizer and urine
The mixture of element.
The purposes of above-mentioned nutritive salt, the nutritive salt is added to hexavalent chromium polluted solid pollutant or liquid pollution
Reinforcing reparation is carried out in thing.
The present invention also provides above-mentioned screening technique and obtains the purposes of nutritive salt, will screen and is used for pollutant described in obtaining
The nutritive salt of microbial augmentation reparation adds carries out reinforcing reparation into the solid pollutant of the pollutant or liquid pollutant.
The present invention can reach following technique effect：
The method according to the invention, below 100mg/kg pollution mediums are reduced to for Cr VI, observe its leachate and cultivating
Growing state on base, the test sample with suitable colony counts is gone out by baseline experiment screening；To the sample screened
Product make leachate, and the nutritive salt that different ratio is added in leachate carries out Shaking culture, pass through plate count and ATP biologies
Fluorescence detector is tested the quantity of microbe colony, comes to carry out preliminary screening to the nutritive salt of different ratio with this；
Then the nutritive salt proportioning group bigger to microbe colony quantity and the concentration of former leachate test Cr VI, are verified with this
The effect of nutritive salt formula.The repairing effect of nutritive salt enhancement microbiological is evaluated according to result above, so as to draw most
Good nutritive salt formula.Then nutritive salt is added in chromium-polluted soil after chemical processing and is stimulated and strengthen indigenous microorganism reparation
Cr VI, strengthen long-time stability.
Directly nutritive salt is added in pollution of chromium medium compared to solid phase reaction and screened, liquid phase reactor more rapidly,
It is more directly perceived.
Embodiment
With reference to specific embodiment, the invention will be further described, so that those skilled in the art can be more preferable
Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
It is that reagent is pure that the present invention, which describes medicament, is provided by Chemical Reagent Co., Ltd., Sinopharm Group.Institute in embodiment
State for examination waste residue containing Cr, the samples of Cr contaminated soils from Shandong, Sichuan, Qinghai, Gansu pollution of chromium place.Sample mainly by
Cr6+Pollution, sample is air-dried, ground respectively, 2mm excessively is standby after sieving.
Embodiment 1
The present embodiment is used for Shandong pollution of chromium place, specific as follows：
Pollutant is Cr slags, in yellow, Cr6+Concentration is 8353mg/kg -9770mg/kg.
Experiment flow is described as follows：
A, 100g contaminated samples are weighed to be fitted into beaker；
B, pH=13.20 of test sample are tested；
C, by electronation stabilization processes, the concentration of Cr VI is reduced to below 100mg/kg；
D, 1000ml deionized waters immersion 6 hours of mixing, mixing is sufficiently stirred within every 2 hours, supernatant is taken after standing 1 hour,
As leachate；
E, 1-2ml leachates are drawn respectively and be seeded in THIOGLYCOLLIC ACID salt broth, nutrient broth, on agar powder, observe bacterium
Fall upgrowth situation；
F, 100ml leachates are separately added into three shaking flasks, 7g compound organic fertilizers and urea are separately added into three shaking flasks
Mixture（C:N:P weight ratio=10:5:1）, 7g methanol and urea mixture（C:N weight ratio=10:5）, 7g glucose and
The mixture of urea（C:N weight ratio=10:5）；
G, Shaking culture tests its flora number, ATP bioluminescence values after 5 days；
H, the hexavalent chromium concentration of leachate former state and nutritive salt group is tested.
Leachate former state hexavalent chromium concentration is 5.3mg/L after electronation is stable.On THIOGLYCOLLIC ACID salt broth
Grow fine, partly show light green color, illustrate that presence can strengthen the flora of repairing hexavalent chromium in leachate.Glucose and
Urea Shaking culture group effect after best 5 days Microflora reach 106/ ml, ATP bioluminescence RLU values improve 1.2 times, and six
Valency chromium concn is changed into 4.7mg/L.Compound organic fertilizer and urea culture group after 5 days Microflora reach 8 × 105/ ml, ATP biology
Fluorescence RLU values improve 1.1 times, and hexavalent chromium concentration is changed into 4.9mg/L.Methanol and urea culture group after 5 days Microflora reach 7
×105/ ml, ATP bioluminescence RLU values improve 1.17 times, and hexavalent chromium concentration is changed into 4.8mg/L.
Embodiment 2
The present embodiment is used for Shandong pollution of chromium place, specific as follows：
Pollutant is Cr contaminated soils, Cr6+Concentration is 376mg/kg -1612mg/kg.
A, 100g contaminated samples are weighed to be fitted into beaker；
B, pH=10.10 of test sample are tested；
C, by electronation stabilization processes, the concentration of Cr VI is reduced to below 100mg/kg；
D, 1000ml deionized waters immersion 8 hours of mixing, mixing is sufficiently stirred within every 2 hours, supernatant is taken after standing 2 hours,
As leachate；
E, 1-2ml leachates are drawn respectively and be seeded in THIOGLYCOLLIC ACID salt broth, nutrient broth, on TTC nutrient agars, see
Examine colony growth situation；
F, 100ml leachates are separately added into three shaking flasks, 7g compound organic fertilizers and urea are separately added into three shaking flasks
Mixture（C:N:P weight ratio=10:5:2）, 7g methanol and urea mixture（C:N weight ratio=10:5）, 7g glucose and
The mixture of urea（C:N weight ratio=10:5）；
G, Shaking culture tests its flora number, ATP bioluminescence values after 7 days；
H, the hexavalent chromium concentration of leachate former state and nutritive salt group is tested.
Leachate former state hexavalent chromium concentration is 1.3mg/L after electronation is stable.On THIOGLYCOLLIC ACID salt broth
Grow fine, largely all become for light green color, illustrate that presence can strengthen the flora of repairing hexavalent chromium in leachate.It is compounded with
Machine is fertile, and Microflora reaches 5 × 10 after best 7 days with urea admixture Shaking culture group effect6/ ml, ATP bioluminescence RLU values
2.5 times are improved, hexavalent chromium concentration is changed into 0.8mg/L.Glucose and urea culture group after 5 days Microflora reach 4 × 106/
Ml, ATP bioluminescence RLU values improve 2.2 times, and hexavalent chromium concentration is changed into 0.9mg/L.Methanol and urea culture group bacterium after 5 days
Group's quantity reaches 2 × 106/ ml, ATP bioluminescence RLU values improve 2.1 times, and hexavalent chromium concentration is changed into 0.9mg/L.
Embodiment 3
The present embodiment is used for Shandong pollution of chromium place, specific as follows：
Pollutant is the mixture of Cr slags and soil, Cr6+Concentration is the mg/kg of 4622mg/kg -5384.
A, 100g contaminated samples are weighed to be fitted into beaker；
B, pH=11.90 of test sample are tested；
C, by electronation stabilization processes, the concentration of Cr VI is reduced to below 100mg/kg；
D, 1000ml deionized waters immersion 7 hours of mixing, mixing is sufficiently stirred within every 2 hours, supernatant is taken after standing 1.5 hours
Liquid, as leachate；
E, 1-2ml leachates are drawn respectively be seeded in nutrient broth, TTC nutrient agars, agar powder, THIOGLYCOLLIC ACID salt fluid culture
On base, colony growth situation is observed；
F, 100ml leachates are separately added into three shaking flasks, 7g compound organic fertilizers and urea are separately added into three shaking flasks
Mixture（C:N:P weight ratio=10:5:1）, 7g prosparols（C:N weight ratio=10:5）, 7g glucose and urea it is mixed
Compound（C:N weight ratio=10:5）；
G, Shaking culture tests its flora number, ATP bioluminescence values after 8 days；
H, the hexavalent chromium concentration of leachate former state and nutritive salt group is tested.
Leachate former state hexavalent chromium concentration is 3.1mg/L after electronation is stable, on THIOGLYCOLLIC ACID salt broth
Grow fine, partly show light green color, illustrate that presence can strengthen the flora of repairing hexavalent chromium in leachate.Glucose and
Urea Shaking culture group effect after best 8 days Microflora reach 2 × 106/ ml, ATP bioluminescence RLU values improve 1.6 times,
Hexavalent chromium concentration is changed into 2.5mg/L.Prosparol culture group after 5 days Microflora reach 106/ ml, ATP bioluminescence RLU
Value improves 1.4 times, and hexavalent chromium concentration is changed into 2.6mg/L.Compound organic fertilizer and urea culture group after 5 days Microflora reach
1.5×106/ ml, ATP bioluminescence RLU values improve 1.5 times, and hexavalent chromium concentration is changed into 2.6mg/L.
Embodiment 4
The present embodiment is used for Gansu pollution of chromium place, specific as follows：
Pollutant is Cr contaminated soils, Cr6+Concentration is 951mg/kg -2463mg/kg.
A, 100g contaminated samples are weighed to be fitted into beaker；
B, pH=10.40 of test sample are tested；
C, by electronation stabilization processes, the concentration of Cr VI is reduced to below 100mg/kg；
D, 1000ml deionized waters immersion 6 hours of mixing, mixing is sufficiently stirred within every 2 hours, supernatant is taken after standing 1 hour,
As leachate；
E, 1-2ml leachates are drawn and are seeded in TTC nutrient agars, agar powder, on THIOGLYCOLLIC ACID salt broth, observe bacterium colony
Upgrowth situation；
F, 100ml leachates are separately added into three shaking flasks, the mixed of 7g humic acid and urea is separately added into three shaking flasks
Compound（C:N:P weight ratio=10:5）, 7g methanol and urea mixture（C:N weight ratio=10:5）, 7g glucose and urea
Mixture（C:N weight ratio=10:5）；
G, Shaking culture tests its flora number, ATP bioluminescence values after 9 days；
H, the hexavalent chromium concentration of leachate former state and nutritive salt group is tested.
Leachate former state hexavalent chromium concentration is 1.5mg/L after electronation is stable, on THIOGLYCOLLIC ACID salt broth
Grow fine, largely all become for light green color, illustrate that presence can strengthen the flora of repairing hexavalent chromium in leachate.Glucose
Reach 4 × 10 with Microflora after best 9 days of urea Shaking culture group effect6/ ml, ATP bioluminescence RLU values improve 2.3
Times, hexavalent chromium concentration is changed into 0.9mg/L.Humic acid and urea culture group after 5 days Microflora reach 3 × 106/ ml, ATP biology
Fluorescence RLU values improve 2.2 times, and hexavalent chromium concentration is changed into 1.0mg/L.Compound organic fertilizer and urea culture group flora number after 5 days
Amount reaches 3 × 106/ ml, ATP bioluminescence RLU values improve 2.2 times, and hexavalent chromium concentration is changed into 1.0mg/L.
Embodiment 5
The present embodiment is used for Qinghai pollution of chromium place, specific as follows：
Pollutant is the mixture of Cr slags and soil, Cr6+Concentration is 6340mg/kg -8463mg/kg.
A, 100g contaminated samples are weighed to be fitted into beaker；
B, pH=12.30 of test sample are tested；
C, by electronation stabilization processes, the concentration of Cr VI is reduced to below 100mg/kg；
D, 1000ml deionized waters immersion 8 hours of mixing, mixing is sufficiently stirred within every 2 hours, supernatant is taken after standing 2 hours,
As leachate；
E, 1-2ml leachates are drawn and is seeded in nutrient broth, TTC nutrient agars, agar powder, THIOGLYCOLLIC ACID salt broth
On, observe colony growth situation；
F, 100ml leachates are separately added into three shaking flasks, 7g compound organic fertilizers and urea are separately added into three shaking flasks
Mixture（C:N:P weight ratio=10:5:2）, 7g methanol and urea mixture（C:N weight ratio=10:5）, 7g glucose and
The mixture of urea（C:N weight ratio=10:5）；
G, Shaking culture tests its flora number, ATP bioluminescence values after 10 days；
H, the hexavalent chromium concentration of leachate former state and nutritive salt group is tested.
Leachate former state hexavalent chromium concentration is 3.5mg/L after electronation is stable, on THIOGLYCOLLIC ACID salt broth
Grow fine, partly show light green color, illustrate that presence can strengthen the flora of repairing hexavalent chromium in leachate.Glucose and
Urea Shaking culture group effect after best 10 days Microflora reach 1.5 × 106/ ml, ATP bioluminescence RLU values improve 1.4
Times, hexavalent chromium concentration is changed into 2.9mg/L.Compound organic fertilizer and urea culture group after 5 days Microflora reach 106/ ml, ATP give birth to
Thing fluorescence RLU values improve 1.3 times, and hexavalent chromium concentration is changed into 3.0 mg/L.Methanol and urea culture group after 5 days Microflora reach
To 106/ ml, ATP bioluminescence RLU values improve 1.2 times, and hexavalent chromium concentration is changed into 3.1mg/L.
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
Enclose not limited to this.The equivalent substitute or conversion that those skilled in the art are made on the basis of the present invention, in the present invention
Protection domain within.Protection scope of the present invention is defined by claims.
Claims (10)
1. the screening technique of a kind of nutritive salt for pollutant microbial augmentation reparation, it is characterised in that comprise the following steps：
1）Culture medium is separately added into the fluid sample containing pollutant, then observes the growing state of flora, carries out baseline sieve
Choosing；
2）Into the fluid sample containing pollutant, different ratio, different types of nutritive salt progress Shaking culture are added respectively, is led to
Plate count and ATP bioluminescences detector are crossed to test the quantity of microbe colony；
3）Detection adds the concentration of Cr VI in the fluid sample containing pollutant after nutritive salt culture, according to result above to battalion
The pollutant repairing effect for supporting salt enhancement microbiological is evaluated, so as to draw optimal nutritive salt formula.
2. screening technique according to claim 1, it is characterised in that the fluid sample containing pollutant is containing pollutant
The leachate of solid sample, the one or more kinds of of surface water of the underground water of contaminants and contaminants appoint
Meaning combination.
3. screening technique according to claim 1, it is characterised in that the pollutant is hexavalent chromium pollutant, containing pollution
The fluid sample of thing is the leachate of hexavalent chromium polluted solid pollutant, hexavalent chromium polluted underground water and hexavalent chromium polluted
Surface water one or more kinds of any combination.
4. screening technique according to claim 3, it is characterised in that the leaching of the hexavalent chromium polluted solid pollutant
The preparation method for going out liquid is as follows：Hexavalent chromium concentration is in below 100mg/kg pollution of chromium solid pollutant or stable through electronation
Hexavalent chromium concentration is reduced to the solid pollutant of below 100mg/kg pollution of chromium after pretreatment, and particle diameter is milled down to below 1cm, adds
Enter the distilled water of deionized water or comparable purity, soak, take supernatant to produce.
5. screening technique according to claim 1, it is characterised in that the culture medium is nutrient broth medium, TTC battalion
Support agar medium, agar powder culture medium, one or more kinds of any combination of THIOGLYCOLLIC ACID salt broth.
6. screening technique according to claim 1, it is characterised in that the nutritive salt is humic acid, prosparol, answered
Close organic fertilizer, urea, corncob, beef jelly, methanol, glucose, one or more kinds of any combination of brewex's grains.
7. the nutritive salt for Cr VI microbial augmentation reparation for screening to obtain using the screening technique described in claim 3,
Characterized in that, the nutritive salt be humic acid, prosparol, compound organic fertilizer, urea, corncob, beef jelly, methanol,
One or more kinds of any combination of glucose, brewex's grains, the carbon-nitrogen mass ratio in the nutritive salt is C:N =10:1~
10。
8. nutritive salt according to claim 7, it is characterised in that the nutritive salt be glucose and urea mixture or
The mixture of person's compound organic fertilizer and urea.
9. the purposes of the nutritive salt described in claim 7, it is characterised in that the nutritive salt is added to hexavalent chromium polluted and consolidated
Reinforcing reparation is carried out in body pollution thing or liquid pollutant.
10. the screening technique described in claim 1 obtains the purposes of nutritive salt, it is characterised in that the use for obtaining screening
Add into the solid pollutant of the pollutant or liquid pollutant and carried out by force in the nutritive salt of pollutant microbial augmentation reparation
Change and repair.
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Cited By (3)
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| CN109316703A (en) * | 2018-11-01 | 2019-02-12 | 南华大学 | A kind of chromium slag passivation preprocess method |
| CN109554298A (en) * | 2019-01-10 | 2019-04-02 | 三峡大学 | The enrichment method of a kind of microbial flora and its in the processing heavy metal-polluted application caught of Cr |
| CN110527519A (en) * | 2019-09-05 | 2019-12-03 | 中国科学院过程工程研究所 | A kind of soil-repairing agent and its application after chromium-polluted soil reparation in inhibition " brightness reversion " |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109316703A (en) * | 2018-11-01 | 2019-02-12 | 南华大学 | A kind of chromium slag passivation preprocess method |
| CN109554298A (en) * | 2019-01-10 | 2019-04-02 | 三峡大学 | The enrichment method of a kind of microbial flora and its in the processing heavy metal-polluted application caught of Cr |
| CN110527519A (en) * | 2019-09-05 | 2019-12-03 | 中国科学院过程工程研究所 | A kind of soil-repairing agent and its application after chromium-polluted soil reparation in inhibition " brightness reversion " |
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Application publication date: 20171124 |