CN107372864A - Composition, preparation method and application containing DHA grease - Google Patents
Composition, preparation method and application containing DHA grease Download PDFInfo
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- CN107372864A CN107372864A CN201610324347.0A CN201610324347A CN107372864A CN 107372864 A CN107372864 A CN 107372864A CN 201610324347 A CN201610324347 A CN 201610324347A CN 107372864 A CN107372864 A CN 107372864A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
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Abstract
The present invention provides a kind of composition containing DHA grease, and the DHA grease is microbe-derived：The DHA content of the DHA grease composition is the wt % of 20 wt % 30, and fusing point is 25 DEG C 35 DEG C.Contain appropriate DHA grease in the fat or oil composition of the present invention, abundant nutrition can be provided in application process.Further, the fusing point of fat or oil composition is higher than room temperature, that is to say, that the fat or oil composition is rendered as solid-state at room temperature, is more convenient during follow-up food applications.Further, when sandwich based food, particularly chocolate cream is made, appropriate DHA content and suitable fusing point cause chocolate cream to have good also to bloom and impervious oily effect.
Description
Technical field
The present invention relates to a kind of fat or oil composition containing DHA grease, preparation method and application.
Background technology
Polyunsaturated fatty acid（Polyunsaturated fatty acids, PUFA）Refer to containing two or two with
The straight chain fatty acid of upper double bond and a length of 18~22 carbon atoms of carbochain, mainly including linoleic acid（LA）, gamma-Linolenic acid（GLA）、
Arachidonic acid（ARA）, eicosapentaenoic acid（EPA）And docosahexaenoic acid（DHA）Deng, wherein, n-3 and n-6 series are more
Unrighted acid plays very crucial effect in Human Physiology, is mainly manifested in as mankind's essential fatty acid：It is right
Preventing for the disease such as promotion, cardiovascular disease, cancer of the eyesight and brain development of infant and children, promotes the life of physiological cells
Long and immunological regulation etc..
DHA and EPA collectively forms the main nutrient composition of n-3 polyunsaturated fatty acids.DHA and EPA has extremely heavy
The Biomedical function wanted, it has also become the focus of medicine and Nutritional studies.DHA/EPA mechanism of action is summarized as：Regulation is thin
Structure, function and the mobility of after birth；To lipid-metabolism, nerve grows, broken up with cynapse, and inflammatory reaction and oxidation are anti-
It should play regulatory role；As the precursor of synthesis inflammatory factor, inhibitory action is played to inflammatory reaction;To angiocardiopathy, itself exempt from
Disease in terms of epidemic disease disease and inflammation, diabetes, cancer and spirit has therapeutic action.
For a long time, many producers are hard by caused DHA in refining process in DHA grease is prepared using microbial method
Fat part is discarded, and has generated largely the wasting of resources.If DHA grease made from fermentation directly is applied into field of food, by
It is relatively low in its fusing point, it can be potentially encountered problem when preparing solid based food.
A kind of oil and fat product containing polyunsaturated fatty acid and application thereof and preparation side are described in CN101252844A
Method.Fat or oil composition based composition as obtained from extracting the DHA grease after fermenting.But said composition is applied
Chocolate center, biscuit sandwich, candy centre and the sandwich middle application effect of waffle are general, in products application and without resisting
The characteristics of white, impervious oily.
The A of CN 103652216 provide a kind of chocolate formula for adding two kinds of unrighted acids of ARA, DHA.It is logical
Cross DHA and ARA with 2：1 form is added in the formula of chocolate, improves the nutritive value of chocolate, and covers well
DHA and ARA its flavour.But DHA and ARA additions are very low, the effect of lifting product nutritive value is not played.
In view of itself fusing point of conventional DHA grease is relatively low, can not directly be used in solid based food, it is necessary to provide one
The composition containing DHA grease of kind of improvement meets the needs of above-mentioned food production.
The content of the invention
The main object of the present invention is to provide a kind of nutritional ingredient height, the good composition containing DHA grease of application effect.
High, the good composition containing DHA grease of application effect it is a further object of the present invention to provide a kind of nutritional ingredient
Preparation method.
It is yet another object of the invention to provide one kind to have a good also to bloom, impervious oily effect containing DHA grease
Food.
To realize above-mentioned main purpose, the present invention provides a kind of composition containing DHA grease, and the DHA grease is micro-
Biological source：The DHA content of the DHA grease composition is 20 wt %-30 wt %, and fusing point is 25 DEG C -35 DEG C.
The composition containing DHA grease of the present invention has the advantages that：Contain appropriate DHA in fat or oil composition
Grease, abundant nutrition can be provided in application process.Further, the fusing point of fat or oil composition is higher than room temperature, that is to say, that
The fat or oil composition is rendered as solid-state at room temperature, is more convenient during follow-up food applications.Further, folder is being made
When heart based food, particularly chocolate cream, appropriate DHA content and suitable fusing point cause chocolate cream to have
Good also to bloom and impervious oily effect.
To realize another object of the present invention, the present invention provides a kind of preparation method of the composition containing DHA grease, bag
Include（1）By microbial fermentation, the grease rich in DHA is obtained；（2）By it is above-mentioned mixed rich in DHA grease with palmitic stearin after, add
Enter lipase and carry out ester exchange；（3）Filter off and remove lipase；Or
The preparation method of composition containing DHA grease, including（1）By microbial fermentation, the grease rich in DHA is obtained；（2）Will
After above-mentioned DHA grease mixes with palmitic stearin and vegetable oil, add sodium methoxide or caustic alcohol carries out ester exchange；（3）It will obtain
Mixture degumming, depickling, decolouring and deodorization.
The above-mentioned mode for using lipase ester exchange or catalyst ester exchange respectively, the present invention can be made contains DHA
The composition of grease, it can meet that different type food is required the difference of raw material.And preparation method is simple, industry is easily realized
Metaplasia is produced.
To realize a further object of the present invention, the present invention provides the foregoing composition containing DHA grease in food, especially
It is the application in sandwich based food.The composition containing DHA grease of the present invention is with the addition of, the nutriture value of product can be lifted
Value, can also have good also to bloom, impervious oily effect.
Embodiment
With reference to instantiation, the present invention is described in further detail.
Embodiment one：Prepare the grease rich in DHA
Incubation step is as follows：200ml activation Secondary Culture bases are loaded in 1000ml shaking flasks, glycerol tube is inoculated with after sterilizing cooling
The algae kind of preservation, 24-72h is cultivated in 28 DEG C, 180rpm shaking tables, obtain primary seed solution.Draw the above-mentioned first order seeds of 10ml
Liquid is inoculated into the 1000ml shaking flasks for the culture medium that spread cultivation equipped with 200ml, the culture acquisition two level in 28 DEG C, 180rpm shaking tables
Seed liquor.And bottle obtains 1L secondary seed solutions and is inoculated into fermentation medium, loaded in fermentation tank, in 28 DEG C, 150rpm is cultivated
96h, microalgae cell is collected by centrifugation.Then centrifugal dehydration is carried out, recycles alkali protease to break cell membrane.Using n-hexane or
The oil component rich in DHA in the organic solvents such as chloroform extraction cell, is then centrifuged for, the crude oil rich in DHA is obtained after precipitation.
By DHA crude oils after degumming, depickling, decolouring, deodorization, DHA refined oils are obtained.
Described activation Secondary Culture base is formed using following component：
Glucose 5g/L, glycerine 5g/L, yeast extract 1g/L, sodium chloride 15g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate
0.5g/L, potassium chloride 0.1g/L, calcium chloride 0.01g/L, ammonium sulfate 0.5g/L, sodium acid carbonate 0.01g/L, manganese chloride
0.01mg/L, frerrous chloride 0.5mg/L, cyanocobalamin 5mg/L, zinc chloride 0.1mg/L, cobalt chloride 0.01mg/L, copper sulphate
0.01mg/L, six hydration nickel sulfate 0.1mg/L, surplus are water, pH=5-8.
Described spreading cultivation is formed with fermentation medium using following component：
Glucose 20g/L, glycerine 10g/L, yeast extract 1g/L, soy peptone 1g/L, corn steep liquor 1g/L, sodium chloride
15g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 0.5g/L, potassium chloride 0.1g/L, calcium chloride 0.01g/L, ammonium sulfate 0.5g/
L, sodium acid carbonate 0.01g/L, manganese chloride 0.01mg/L, frerrous chloride 0.5mg/L, cyanocobalamin 5mg/L, zinc chloride 0.1mg/
L, cobalt chloride 0.01mg/L, copper sulphate 0.01mg/L, six hydration nickel sulfate 0.1mg/L, pantothenic acid 0.1mg/L, biotin 0.1mg/
L, surplus are water, pH=5-8.
Embodiment two：Prepare the composition containing DHA grease
Hereinafter, by different method and process conditions, the composition containing DHA grease of the invention is prepared.Such as not special theory
Bright, following percentages are mass percent（wt%）.
Method one
(1) DHA grease 78kg made from embodiment one is provided, after testing, DHA content is 55% in the grease, and fusing point is 7.2 DEG C.
(2) by DHA grease and 20kg palmitic stearins, it is mixed in reactor, stirs, adds lipase
6.28kg, ester exchange 1 hour at 45 DEG C；
(3) lipase is filtered to remove, obtains the composition containing DHA grease, its content of triglyceride is 98.1%.After testing, should
In composition containing DHA grease, myristic acid（C14:0）Content be 3%, palmitic acid（C16:0）Content be 32%, oleic acid
（C18:1）Content be 7%, eicosapentaenoic acid（EPA）Content be 0.8%, the content of clupanodonic acid (DPA) is
11%, docosahexaenoic acid（DHA）Content be 30.1%.Further detection, being somebody's turn to do the composition fusing point containing DHA grease is
25.1℃.The fat or oil composition can add sapn in right amount（65）.
Method two
(1) DHA grease 10kg made from embodiment one is provided, after testing, DHA content is 45% in the grease, fusing point 13.2
℃。
(2) by DHA grease and 2.6kg palmitic stearins, it is mixed in reactor, stirs, adds lipase
0.625kg, ester exchange 1 hour at 45 DEG C；
(3) lipase is filtered to remove, obtains the composition containing DHA grease, its content of triglyceride is 98.1%.After testing, should
In composition containing DHA grease, myristic acid（C14:0）Content be 5%, palmitic acid（C16:0）Content be 35%, oleic acid
（C18:1）Content be 6%, eicosapentaenoic acid（EPA）Content be 0.7%, the content of clupanodonic acid (DPA) is
10%, docosahexaenoic acid（DHA）Content be 28%.Further detection, it is 26.5 to be somebody's turn to do the composition fusing point containing DHA grease
℃.The fat or oil composition can add sapn in right amount（65）.
Method three
(1) DHA grease 4kg made from embodiment one is provided, after testing, DHA content is 40% in the grease, and fusing point is 20.4 DEG C.
(2) by DHA grease and 1kg palmitic stearins, it is mixed in reactor, stirs, adds lipase 0.26kg,
Ester exchange 1.5 hours at 45 DEG C；
(3) lipase is filtered to remove, obtains the composition containing DHA grease, its content of triglyceride is 98.6%.After testing, should
In composition containing DHA grease, myristic acid（C14:0）Content be 8%, palmitic acid（C16:0）Content be 40%, oleic acid
（C18:1）Content be 5%, eicosapentaenoic acid（EPA）Content be 0.5%, the content of clupanodonic acid (DPA) is
8%, docosahexaenoic acid（DHA）Content be 25%.Further detection, it is 30 DEG C to be somebody's turn to do the composition fusing point containing DHA grease.
The fat or oil composition can add sapn in right amount（65）.
Method four
(1) DHA grease 1kg made from embodiment one is provided, after testing, DHA content is 35% in the grease, and fusing point is 22.2 DEG C.
(2) by DHA grease and 0.26kg palmitic stearins, it is mixed in reactor, stirs, adds lipase
0.05kg, ester exchange 2 hours at 45 DEG C；
(3) lipase is filtered to remove, obtains the composition containing DHA grease, its content of triglyceride is 98.9%.After testing, should
In composition containing DHA grease, myristic acid（C14:0）Content be 10%, palmitic acid（C16:0）Content be 44%, oleic acid
（C18:1）Content be 3%, eicosapentaenoic acid（EPA）Content be 0.4%, the content of clupanodonic acid (DPA) is
6%, docosahexaenoic acid（DHA）Content be 20%.Further detection, it is 35 DEG C to be somebody's turn to do the composition fusing point containing DHA grease.
The fat or oil composition can add sapn in right amount（65）.
Method five
(1) DHA grease 100kg made from embodiment one is provided, after testing, DHA content is 55% in the grease, fusing point 6.8
℃。
(2) by 90 kilograms of DHA grease, 54 kilograms of palmitic stearin, 2.2 kilograms of rapeseed oil, 2.6 kilograms of grades of soybean oil are 60
Melt at DEG C and stir, then add sodium methoxide or caustic alcohol in 80 DEG C of ester exchanges 30 minutes,
（3）Progress degumming, depickling, decolouring, deodorization obtain composition containing DHA grease, and its content of triglyceride is 99.2%.Through inspection
Survey, should contain in DHA grease composition, myristic acid（C14:0）Content be 3%, palmitic acid（C16:0）Content be 32%, firmly
Resin acid（C18:0）Content be 5%, oleic acid（C18:1）Content be 11%, eicosapentaenoic acid（EPA）Content be 0.5%,
The content of clupanodonic acid (DPA) is 11%, docosahexaenoic acid（DHA）Content be 30.2%.Further detection,
The composition fusing point for containing DHA grease is 25.2 DEG C.The fat or oil composition can add sapn in right amount（65）.
Method six
(1) DHA grease 10kg made from embodiment one is provided, after testing, DHA content is 45% in the grease, fusing point 13.1
℃。
(2) by 8.8 kilograms of DHA grease, 5.8 kilograms of palmitic stearin, 0.22 kilogram of rapeseed oil, 0.26 kilogram of soybean oil etc.
Melt and stir at 60 DEG C, then add sodium methoxide or caustic alcohol in 80 DEG C of ester exchanges 30 minutes.
（3）Progress degumming, depickling, decolouring, deodorization obtain composition containing DHA grease, and its content of triglyceride is 99.2%.
After testing, should contain in DHA grease composition, myristic acid（C14:0）Content be 4%, palmitic acid（C16:0）Content be
34%, stearic acid（C18:0）Content be 5%, oleic acid（C18:1）Content be 11%, eicosapentaenoic acid（EPA）Content be
0.5%, the content of clupanodonic acid (DPA) is 9%, docosahexaenoic acid（DHA）Content be 28%.Further inspection
Survey, it is 26.8 DEG C to be somebody's turn to do the composition fusing point containing DHA grease.The fat or oil composition can add sapn in right amount（65）.
Method seven
(1) DHA grease 5kg made from embodiment one is provided, after testing, DHA content is 40% in the grease, and fusing point is 20.5 DEG C.
(2) it is 4.5 kilograms of DHA grease, 2.75 kilograms of palmitic stearin, 0.125 kilogram of rapeseed oil, soybean oil 0.125 is public
Jin, which waits, to be melted and stirs at 60 DEG C, then adds sodium methoxide or caustic alcohol in 80 DEG C of ester exchanges 60 minutes；
（3）Progress degumming, depickling, decolouring, deodorization obtain composition containing DHA grease, and its content of triglyceride is 98.5%.Through inspection
Survey, should contain in DHA grease composition, myristic acid（C14:0）Content be 4%, palmitic acid（C16:0）Content be 36%, firmly
Resin acid（C18:0）Content be 3%, oleic acid（C18:1）Content be 9%, eicosapentaenoic acid（EPA）Content be 0.4%, two
The content of eicosapentaenoic acid (DPA) is 7%, docosahexaenoic acid（DHA）Content be 25%.Further detection, this contains
The composition fusing point of DHA grease is 30 DEG C.
Method eight
(1) DHA grease 1kg made from embodiment one is provided, after testing, DHA content is 35% in the grease, and fusing point is 22 DEG C.
(2) it is 0.9 kilogram of DHA grease, 0.56 kilogram of palmitic stearin, 0.026 kilogram of rapeseed oil, soybean oil 0.022 is public
Jin, sapn（65）Melt and stir Deng at 60 DEG C, then add sodium methoxide or caustic alcohol in 80 DEG C of ester exchanges 90 minutes；
（3）Progress degumming, depickling, decolouring, deodorization obtain composition containing DHA grease, and its content of triglyceride is 99.4%.Through inspection
Survey, should contain in DHA grease composition, myristic acid（C14:0）Content be 5%, palmitic acid（C16:0）Content be 40%, firmly
Resin acid（C18:0）Content be 2%, oleic acid（C18:1）Content be 8%, eicosapentaenoic acid（EPA）Content be 0.2%, two
The content of eicosapentaenoic acid (DPA) is 5%, docosahexaenoic acid（DHA）Content be 20%.Further detection, this contains
The composition fusing point of DHA grease is 35 DEG C.
Embodiment three：Prepare the chocolate cream containing DHA grease
The formula for making chocolate shell is provided：Substitute of cocoa fat 35kg, cocoa power 15kg, Icing Sugar 35kg, skimmed milk power 15kg, phosphorus
Fat 0.5kg, 0.02 kilogram of vanillic aldehyde.
The formula for making chocolate center is provided：Composition 40kg containing DHA grease made from embodiment two, Icing Sugar
40kg, skimmed milk power 20kg .5 kilograms of phosphatidase 0, vanillic aldehyde 0.02kg.
It is prepared by chocolate shell material and core material
It is prepared by chocolate shell material：Matched by above-mentioned raw materials, grease is placed in 45-50 DEG C of water-bath, heating and melting；When grease melts
After change, it is well mixed to add Icing Sugar, milk powder, cocoa power etc.；Fine grinding, control material particle diameter is below 25 μm, 40-45 DEG C of insulation,
It is standby.
It is prepared by chocolate core material：Matched by above-mentioned raw materials, grease is placed in 45-50 DEG C of water-bath, heating and melting；Work as oil
After fat melts, it is well mixed to add Icing Sugar, skimmed milk power etc.；Fine grinding, control material particle diameter is below 25 μm, and 30 DEG C of insulations are standby
With.
It is prepared by chocolate center
The chocolate shell material of insulation is injected in 25 DEG C or so of mould, sloughed the bubble in material by vibration, then by
Inversion set topples over mould 180 ° of reversions of work, unnecessary material in removing model, and edge of model is sticked material and scraped by cleaning roller
Wipe totally, make 180 ° of resets through inversion set after.Mould enters cooling tunnel, and the material of cooled and solidified forms one in model
The thin chocolate shell of layer.
Mould is delivered to below heart body injection molding machine by conveyer belt, is quantitatively injected chocolate core material in shell, through bobbing machine
Heart material is uniformly dispersed, mould is again introduced into cooling tunnel, is sent back to again below chocolate mass injection molding machine after core material condensation.
Before entering below injection molding machine, one electric heater of configuration heats surface of material, material is slightly melted, and then quantitative injection is skilful
Gram force material, unnecessary material is scraped off after vibrating machine vibration, mould is finally admitted to a Multilayer vertical refrigerator, carry out cooling and
The demoulding, enters back into packaging system, obtains chocolate cream product.
Choose the DHA grease after the direct fermentation of embodiment 1（DHA content 45%）For 1#, the beneficial sandwich oil of Hai Jiali for 2#, no
Two sandwich oil are 3#, and chocolate cream is prepared according to above-mentioned identical technique, and with composition 4# of the present invention containing DHA grease
The chocolate cream of preparation carries out accelerated shelf life testing contrast.
Experiment condition：Chocolate is placed in 20 DEG C of -33 DEG C of circulation high/low temperature baking ovens, wherein 12h at 20 DEG C, at 33 DEG C
12h, it is as a result as follows：
| Cycle | 1# | 2# | 3# | 4# |
| After 7 days | Frosting | Non- frosting | Non- frosting | Non- frosting |
| After 14 days | Frosting | Non- frosting | Frosting | Non- frosting |
| After 21 days | Frosting | Frosting | Frosting | Non- frosting |
| After 28 days | Frosting | Frosting | Frosting | Non- frosting |
Studies of chocolate blooming is one of main quality evaluation index of chocolate-like products, and frosting not only causes to product in appearance
Influence, and mouthfeel can be made substantially to be deteriorated and shorten the shelf life of product.By as above contrasting as can be seen that the present invention contains
The composition of DHA grease, provide not only the fat or oil composition of great nutritive value, also has more preferable also to bloom, resists
Permeability effect.
Example IV：Prepare the sandwich biscuits containing DHA grease
Biscuit sandwich formula is provided：The present invention composition 35kg containing DHA grease, Icing Sugar 40kg, glucose 10kg, milk powder
10kg, cocoa power 5kg, phosphatide, appropriate essence.
By composition grease of the present invention containing DHA grease, heating and melting and being put into a mixing bowl stirs evenly at 40 DEG C, so
Icing Sugar, glucose, milk powder, cocoa power, phosphatide, essence etc. are added afterwards makes biscuit（It is commercially available）It is sandwich.It is while hot that sandwich feed liquid is equal
It is even to be applied to biscuit surface, take another piece of same biscuit surface to cover, suitably press down, 2h is cooled down at 10 DEG C, obtain sandwich
Biscuit.
According to the similar mode of embodiment three carry out contrast experiment after find, using group of the present invention containing DHA grease
Biscuit sandwich prepared by compound still has more preferable impervious oily effect.
Above using lipase ester exchange or the means of chemically catalyzed interesterification, the application performance of DHA grease is improved, is contained
There are DHA and the oil component of physicochemical property uniqueness.The oil component it is harder at normal temperatures and under high temperature can fast melt,
So as to have good application effect in chocolate center, biscuit sandwich, candy centre, the sandwich medium food of waffle.According to phase
As contrast experiment, it can be seen that the composition containing DHA grease of the invention after these food are prepared, it may have it is more preferable impervious
Oily effect.
Claims (7)
1. the composition containing DHA grease, the DHA grease is microbe-derived, it is characterised in that：The DHA grease combination
The DHA content of thing is 20 wt %-30 wt %, and fusing point is 25 DEG C -35 DEG C.
2. the preparation method of the composition containing DHA grease described in claim 1, comprises the following steps：
（1）By microbial fermentation, the grease rich in DHA is obtained；
（2）By it is above-mentioned mixed rich in DHA grease with palmitic stearin after, add lipase carry out ester exchange；
（3）Filter off and remove lipase.
3. the preparation method of the composition according to claim 2 containing DHA grease, it is characterised in that：
（1）By microbial fermentation, the grease rich in DHA is obtained, DHA content 35%-55wt% in the grease；
（2）By above-mentioned 3.8 are pressed rich in DHA grease and palmitic stearin:1 to 4:After 1 ratio mixing, add lipase and carry out ester
Exchange, wherein, the proportion 1 of the weight of lipase and the weight of compound lard:22 to 1:25, the time of ester exchange reaction is 1-2
Hour；
（3）Filter off and remove lipase.
4. the preparation method of the composition containing DHA grease described in claim 1, comprises the following steps：
By microbial fermentation, the grease rich in DHA is obtained；
After above-mentioned DHA grease is mixed with palmitic stearin and vegetable oil, add sodium methoxide or caustic alcohol carries out ester exchange；
Mixture degumming, depickling, decolouring and the deodorization that will be obtained.
5. the preparation method of the composition according to claim 4 containing DHA grease, it is characterised in that：
By microbial fermentation, the grease rich in DHA is obtained, DHA content is 35%-55wt% in the grease；
After above-mentioned DHA grease, palmitic stearin, rapeseed oil, soybean oil are mixed with default ratio, sodium methoxide or ethanol are added
Sodium carries out ester exchange, and the reaction time is 30-90 minutes；
Mixture degumming, depickling, decolouring and the deodorization that will be obtained.
6. application of the composition containing DHA grease in food described in claim 1.
7. application of the composition according to claim 6 containing DHA grease in food, the food is eaten for sandwich class
Product, including chocolate center, biscuit sandwich, candy centre and waffle are sandwich.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610324347.0A CN107372864A (en) | 2016-05-17 | 2016-05-17 | Composition, preparation method and application containing DHA grease |
| PCT/CN2017/084742 WO2017198176A1 (en) | 2016-05-17 | 2017-05-17 | Composition comprising dha grease, preparation method and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610324347.0A CN107372864A (en) | 2016-05-17 | 2016-05-17 | Composition, preparation method and application containing DHA grease |
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| CN107372864A true CN107372864A (en) | 2017-11-24 |
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| CN201610324347.0A Pending CN107372864A (en) | 2016-05-17 | 2016-05-17 | Composition, preparation method and application containing DHA grease |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115715559A (en) * | 2022-11-08 | 2023-02-28 | 嘉必优生物技术（武汉）股份有限公司 | Method for enriching DHA in grease |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11419350B2 (en) | 2016-07-01 | 2022-08-23 | Corbion Biotech, Inc. | Feed ingredients comprising lysed microbial cells |
| AU2019397469A1 (en) * | 2018-12-13 | 2021-07-08 | Cargill, Incorporated | Modified triglyceride including omega-3 fatty acid residue |
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2017
- 2017-05-17 WO PCT/CN2017/084742 patent/WO2017198176A1/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485364A (en) * | 2009-02-11 | 2009-07-22 | 湖北友芝友生物科技有限公司 | Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange |
| CN102965182A (en) * | 2012-11-27 | 2013-03-13 | 新奥科技发展有限公司 | Method for extracting grease from schizochytrium |
| CN103815104A (en) * | 2013-09-30 | 2014-05-28 | 安徽康尔美油脂有限公司 | Method for preparing cocoa butter equivalent by using chemical transesterification method |
| CN104938737A (en) * | 2015-05-29 | 2015-09-30 | 华南理工大学 | Modified cocoa butter, functional chocolate and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115715559A (en) * | 2022-11-08 | 2023-02-28 | 嘉必优生物技术（武汉）股份有限公司 | Method for enriching DHA in grease |
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| WO2017198176A1 (en) | 2017-11-23 |
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