CN107349196A - A kind of medicine for improving glucose -lipid metabolism disorder and vascular function and preparation method thereof - Google Patents
A kind of medicine for improving glucose -lipid metabolism disorder and vascular function and preparation method thereof Download PDFInfo
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- CN107349196A CN107349196A CN201710563564.XA CN201710563564A CN107349196A CN 107349196 A CN107349196 A CN 107349196A CN 201710563564 A CN201710563564 A CN 201710563564A CN 107349196 A CN107349196 A CN 107349196A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
- A61K31/343—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
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Abstract
The invention belongs to medicine and pharmacology technical field, discloses a kind of medicine for improving glucose -lipid metabolism disorder and vascular function and preparation method thereof, the medicine for improving glucose -lipid metabolism disorder and vascular function is root of red-rooted salvia phenolic acid B；The root of red-rooted salvia phenolic acid B molecular formula is：C36H30O16；Preparation method includes：Red rooted salvia is ground into fine powder, crosses the mesh sieve of 20 mesh~60；Ethanol solution is added, is soaked at less than 5 DEG C, ultrasonic extraction, extraction process temperature is less than 25 DEG C；Extract solution centrifuges, filtering, and ethanol is recovered under reduced pressure to extract solution without alcohol taste, extract solution concentration, filtering；Extract solution is clarified, first passes through macroporous resin enrichment, then carries out column chromatography purifying, is freeze-dried, obtains root of red-rooted salvia phenolic acid B extract.The present invention has good protective effect to myocardial infarction, while can significantly improve the damage of Ischemic/reperfusion；Platelet aggregation-against prevents thrombosis；Improve microcirculation；Anti-oxidative damage etc. acts on.
Description
Technical field
The invention belongs to medicine and pharmacology technical field, more particularly to a kind of medicine for improving glucose -lipid metabolism disorder and vascular function
And preparation method thereof.
Background technology
Diabetes and obesity are to influence the major disease of Chinese health, and China has had more than 1.14 hundred million glycosuria at present
Patient and more than 7,000 ten thousand obese patients.Fat closely related with angiocardiopathy and diabetes, glucose -lipid metabolism disorder is in artery
The generation of atherosis, play vital effect in developing.OASIS researchs, the research of Italian acute myocardial infarction AMI
(GISSI-2, GISSI-3), SHOCK research etc. confirms that the ratio of diabetic's generation myocardial infarction is increasing year by year.
OASIS studies have shown that diabetes make unstable angina, the death rate of non-Q ripples heart infarction patient increase by 57%.SHOCK is studied then
It was found that the patient of heart infarction Complicated with Cardiogenic Shock receives in reconstructing blood vessel, the dead relative risk (RR) of diabetic is
1.36, it is seen that the generation of diabetes and atherosclerosis is closely related, strengthens the study of incident mechanism to glucose -lipid metabolism disorder,
Actively seeking effective prophylactico-therapeutic measures has important strategic importance.
At present, still there is remaining risk under the treatment of the hypoglycemic, lipid-regulation medicine of specification, so far for diabetes medicament
The prognosis that Patients with Cardiovascular/Cerebrovascular Diseases whether can be effectively improved still suffers from dispute.The current glucose -lipid metabolism disorder medicine that improves is controlled
The inferior position for the treatment of is that side effect is big, and patient is discontinued the situation for the treatment of because not being resistant to drug side-effect.
In summary, the problem of prior art is present be：The inferior position of glucose -lipid metabolism disorder drug therapy be present in prior art
I.e. side effect is big, and patient is discontinued treatment because not being resistant to drug side-effect.
The content of the invention
The problem of existing for prior art, the invention provides a kind of medicine for improving glucose -lipid metabolism disorder and vascular function
Thing and preparation method thereof.
The present invention is achieved in that a kind of medicine for improving glucose -lipid metabolism disorder and vascular function, the improvement glycolipid
The medicine of metabolic disorder and vascular function is root of red-rooted salvia phenolic acid B；The root of red-rooted salvia phenolic acid B molecular formula is：C36H30O16；Molecular weight is：
718.62g/mol。
Further, the root of red-rooted salvia phenolic acid B chemical structural formula is：
Another object of the present invention is to provide a kind of process for preparing medicine bag for improving glucose -lipid metabolism disorder and vascular function
Include following steps：
Step 1, red rooted salvia is ground into fine powder, crosses the mesh sieve of 20 mesh~60；
Step 2, ethanol solution is added, is soaked at less than 5 DEG C, ultrasonic extraction, extraction process temperature is less than 25 DEG C；
Step 3, extract solution centrifugation, filtering, ethanol is recovered under reduced pressure to extract solution without alcohol taste, extract solution concentration, filtering；
Step 4, extract solution is clarified, first passes through macroporous resin enrichment, then carry out column chromatography purifying, it is big to obtain purity
In 90% phenolic acid B fraction；Freeze-drying, obtains root of red-rooted salvia phenolic acid B extract.
Further, fine powder is formed using biochemical method or Mechanical Crushing in the step 1.
Further, in the step 2, the ethanol solution amount of addition is 8 times~10 times volumes of medicinal material, and the ethanol is molten
Liquid is the ethanol solution of percent by volume 40%~80%.
Further, in the step 2, soak time is 12 hours, and the ultrasonic extraction time is 20min~60min.
Further, the extract solution in the step 3 is concentrated using reduced vacuum except alcohol concentration, during concentration, extract solution temperature
Less than 60 DEG C.
Further, in the step 4, macroreticular resin is used by the macroporous resin enrichment：Nonpolar or low pole
Macroreticular resin；Described nonpolar or low pole macroreticular resin is AB-8, D-101 or HPD750.
Further, in the step 4, the chromatographic column be dynamic axial pressurize chromatographic column, chromatographic column filler be silica gel or
Polyamide；
The step 4 is specially：Extract solution is clarified, first passes through macroporous resin enrichment, then carries out column chromatography purifying,
Removal of impurities is eluted with the ethanol solution of the water of 1 times~3 times column volumes, 3 times~5 times column volumes 20%~30% successively, is then used again
40%~50% ethanol solution elution root of red-rooted salvia phenolic acid B, merges the eluent containing root of red-rooted salvia phenolic acid B, after concentrated drying, obtains
Purity is more than 90% root of red-rooted salvia phenolic acid B dry powder.
Advantages of the present invention and good effect are：
The invention discloses a kind of medicine for treating glucose -lipid metabolism disorder and vascular function and preparation method thereof, root of red-rooted salvia phenolic acid
B is the important activity composition in China's traditional Chinese medicine red sage root, has a variety of pharmacological activity, has to myocardial infarction and protects well
Shield acts on, while can significantly improve the damage of Ischemic/reperfusion；Platelet aggregation-against prevents thrombosis；Improve
Microcirculation；Anti-oxidative damage etc. acts on, and effect is good and few side effects, medicine of the invention are directed to glucose -lipid metabolism disorder artery congee
Sample hardening morbidity principal mechanisms play protective effect, for glucose -lipid metabolism disorder atherosclerosis treatment bring it is new
Therapeutic strategy；Solve that current hypoglycemic, the inferior position i.e. side effect of Drug Therapy of Regulating Lipidemia are big, and patient is not because being resistant to medicine simultaneously
Side effect and the situation for treatment of being discontinued.Being proved root of red-rooted salvia phenolic acid B by experiment can be by adjusting PPAR γ acceptors to glycolipid metabolism
Disorderly atherosclerosis plays potential protective effect, therefore root of red-rooted salvia phenolic acid B is controlling for glucose -lipid metabolism disorder atherosclerosis
Treatment brings new dawn.
Root of red-rooted salvia phenolic acid B is the hydrophilic compounds of one big polarity, the phenolic hydroxyl group and carboxyl of existing big polarity in its structure, again
The small polarity benzene ring structure of certain amount be present, immersion of the aqueous solution of alcohol to medicinal material is beneficial to the molten of root of red-rooted salvia phenolic acid B material
Go out, while substantially reduce the dissolution of fat soluble ingredient of red sage root, offer facility is isolated and purified for next step；Other root of red-rooted salvia phenolic acid B
It is unstable, the especially low concentration root of red-rooted salvia phenolic acid B aqueous solution, place 1 hour at 30 DEG C, have 5% degraded, root of red-rooted salvia phenolic acid B is in ethanol
Stability in the aqueous solution is greater than pure water, and the stabilization of the root of red-rooted salvia phenolic acid B of dissolution is more beneficial under low-temperature condition.Profit of the invention
With this principle, using low temperature cold soaking combination ultrasonic extraction, successful high efficiency extraction root of red-rooted salvia phenolic acid B.
The dynamic axial pressurization chromatographic technology of the present invention is to fill post by the up and down motion of piston, maintains post pressure and unloads
Post, piston periphery are equipped with the sealing ring of particular design, piston can be allowed freely up and down to slide, while and can keeps high close
Envelope pressure.What piston movement and pressure maintained to lean on is hydraulic pressure, and hydraulic power is more more stable than the spring powered of axial compression column, more equal
It is even.These technologies cause it have cost-effective, long lifespan, post effect high, symmetry and it is reproducible the characteristics of, can load
Diameter range is big (50mm~1000mm), and keeps the separating effect suitable with analytical column.
Brief description of the drawings
Fig. 1 is the process for preparing medicine flow of improvement glucose -lipid metabolism disorder and vascular function provided in an embodiment of the present invention
Figure.
Fig. 2 is influence of the root of red-rooted salvia phenolic acid B provided in an embodiment of the present invention to db/db mouse blood pressure, body weight and fasting blood-glucose
Schematic diagram (compared with C57BL/KsJ groups, * * P ＜ 0.01；Compared with db/db Cont,#P ＜ 0.05,##P ＜ 0.01).
Fig. 3 is the thoracic aorta endothelium that root of red-rooted salvia phenolic acid B provided in an embodiment of the present invention is induced db/db mouse Ach
Dependence diastolic function influence schematic diagram (compared with C57BL/KsJ groups, * * P ＜ 0.01；Compared with db/db Cont,##P ＜
0.01)。
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and do not have to
It is of the invention in limiting.
At present, still there is remaining risk under the treatment of the hypoglycemic, lipid-regulation medicine of specification, so far for diabetes medicament
The prognosis that Patients with Cardiovascular/Cerebrovascular Diseases whether can be effectively improved still suffers from dispute.The current glucose -lipid metabolism disorder medicine that improves is controlled
The inferior position for the treatment of is that side effect is big, and patient is discontinued the situation for the treatment of because not being resistant to drug side-effect.
It is described in detail below in conjunction with the accompanying drawings and to the application principle of the present invention.
The medicine of the treatment glucose -lipid metabolism disorder of the embodiment of the present invention is root of red-rooted salvia phenolic acid B, and root of red-rooted salvia phenolic acid B molecular formula is：
C36H30O16；Molecular weight is： 718.62g/mol.
Chemical structural formula is：
As shown in figure 1, the improvement glucose -lipid metabolism disorder of the embodiment of the present invention and the process for preparing medicine of vascular function include
Following steps：
S101：The red rooted salvia 100g for being broken into fine powder is taken, adds 8 times of ethanol of volume (V/W) 60%, in 5 DEG C of immersions
24 hours, then ultrasonic extraction 30min, extraction process, which is cooled with circulating water, controls extract solution temperature to be no more than 20 DEG C, centrifugation, carries
Liquid recovery ethanol is taken to without alcohol taste.
S102：Macroporous resin adsorption separates：Clarification without D-101 type macroporous resin columns on alcohol taste extract solution, loading sample (with
Crude drug dry weight calculates) with the ratio between amount of resin it is 1: 2 (W/V), first with the water elution impurity of 2 times of column volumes, then with 5 times of cylinders
The ethanol water that product concentration is 30% elutes.
S103：Process-scale chromatography column chromatography：The ethanol water of macroreticular resin 30% elutes part, and concentration removes alcohol, upper polyamide
(200~400 mesh) dynamic axial is pressurizeed chromatographic column, is first cleaned with sour water, successively with 3 times of ethanol elutions of column volume 20%, 5 times
The ethanol elution of column volume 40%, finally with 3 times of ethanol elutions of column volume 50%.
S104：50% ethanol eluate is concentrated in vacuo under conditions of temperature 60 C, vacuum -0.1Mpa, and concentrate is cold
It is lyophilized it is dry after, obtain the root of red-rooted salvia phenolic acid B that purity is 91.8%.
The application effect of the present invention is explained in detail with reference to experiment.
(1) experimental animal packet and intervention：8 week old male db/db mouse, are purchased from Nanjing University's model experiment and move by 20
Thing center (background C57BL/KsJ), divides db/db control groups (db/db Cont) and db/db root of red-rooted salvia phenolic acid B groups, every group 10
Mouse；Separately 10 of the same age, other C57BL/KsJ of the same sex are taken as wild type control.The experimental method established according to the past, db/
Db root of red-rooted salvia phenolic acid B group is administered (root of red-rooted salvia phenolic acid B is dissolved in physiological saline) by 100mg/kg/day dosage through gastric perfusion needle.db/db
Cont groups and C57BL/KsJ control groups then give the physiological saline gavage of same volume.All mouse divide cage to feed, ad lib
Drinking-water, first carry out continuous after adaptability is fed 1 week intervene 6 weeks.Naturally daylighting round the clock, 22~24 DEG C of room temperature.
(2) instrument and reagent：Dihydro second ingot (Dihydroethidium, DHE), 4,5- diglycyl acetic acid fluoresceins
(4-amino-5 methylamino-2,7-difluorofluorescein diacetate, DAF-2DA), phyenlephrinium
(Phenylephrine, PE), nitroglycerin (Nitroglycerin, NTG), acetylcholine (Acetylcholine, Ach) etc.
Reagent is purchased from Sigma companies.DMT 620M capilaries tension measuring device (DMT companies of Denmark)；Mouse non-invasive blood pressure measuring (BP-
98A, Softron, JAPAN)；Blood glucose meter and blood sugar test paper (Johnson ＆ Johnson of the U.S.).
(3) observation index：A body weight, blood pressure and rat-tail fasting blood sugar are surveyed weekly.With 10% water after continuously intervening 6 weeks
Aorta pectoralis promoting circulation of blood pipe Function detection is taken after closing chloralization；DHE dyeing observation endangium superoxide anions are horizontal.
(4) DHE and DAF-2DA dyeing observation endangium superoxide anion and NO are horizontal：With reference to this research team previously
The experimental method of foundation, with reference to reagent specification, carry out DAF-2DA (observation endarterium NO is horizontal) and DHE (observation arteries
Inner membrance superoxide anion is horizontal) dyeing, observed, taken pictures under inverted fluorescence microscope, calculate average optical density (OD
Value).
The present invention adjusts PPAR γ acceptors by root of red-rooted salvia phenolic acid B and glucose -lipid metabolism disorder atherosclerosis is played potentially
Protective effect.The red sage root is the traditional blood-activating and stasis-removing in China, Asia and western countries is widely used to, for treating brain
Vascular diseases, coronary artery disease, diabetes and diabetic complication.Root of red-rooted salvia phenolic acid B is the important activity composition in the red sage root,
With a variety of pharmacological activity, there is good protective effect to myocardial infarction, while can significantly improve myocardial ischemia/again
The damage of perfusion；Platelet aggregation-against prevents thrombosis；Improve microcirculation；Anti-oxidative damage etc. acts on, few side effects, should
Medicine is directed to the principal mechanisms play protective effect of glucose -lipid metabolism disorder incidence of atherosclerosis, is moved for glucose -lipid metabolism disorder
The treatment of pulse atherosclerosis brings new therapeutic strategy.Binding experiment result, root of red-rooted salvia phenolic acid B can prevent fat generation and improve
The effect of glycolipid metabolism
With reference to specific embodiment, the invention will be further described.
Embodiment 1：
Root of red-rooted salvia phenolic acid B (100mg/kg/day) can significantly reduce the body weight (A schemes in Fig. 2) and fasting blood-glucose of db/db mouse
Horizontal (B schemes in Fig. 2), compared with db/db control mices, the equal ＜ 0.05 of P；Db/db mouse blood pressure is with its wild type control without aobvious
Write sex differernce, P ＞ 0.05 or P ＜ 0.01.Compared with C57BL/KsJ wild type control mices, the rat-tail of db/db mouse is shunk
Blood pressure significantly raises, P ＜ 0.01；Root of red-rooted salvia phenolic acid B intervention is to the systolic pressure of mouse without significant impact, P ＞ 0.05；As a result see
C schemes in Fig. 2.
Embodiment 2：
Compared with wild type control C57BL/KsJ, db/db mouse aorta inner membrance NO levels are remarkably decreased, super oxygen the moon from
Sub horizontal significantly rising, the equal ＜ 0.01 of P；Root of red-rooted salvia phenolic acid B intervention can significantly reduce superoxide anion level, increase endangium
NO is horizontal, the equal ＜ 0.01 of P.
Embodiment 3：
Vascular function experiment prompting, compared with wild type control C57BL/KsJ, db/db mouse thoracic aorta endotheliums
Dependence diastolic function is substantially damaged, P ＜ 0.01；There was no significant difference between non-two groups of endothelium-dependent dilation function, P ＞
0.05；Root of red-rooted salvia phenolic acid B can significantly improve endothelium-dependent vascular relaxation, and P ＜ 0.01 (scheme) see A in Fig. 3；To non-endothelium
Dependence diastolic function does not make significant difference, and P ＞ 0.05 (scheme) see B in Fig. 3
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (9)
- A kind of 1. medicine for improving glucose -lipid metabolism disorder and vascular function, it is characterised in that the improvement glucose -lipid metabolism disorder and The medicine of vascular function is root of red-rooted salvia phenolic acid B；The root of red-rooted salvia phenolic acid B molecular formula is：C36H30O16；Molecular weight is：718.62g/mol.
- 2. improve the medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 1, it is characterised in that the danshensu Sour B chemical structural formulas are：
- 3. a kind of process for preparing medicine as claimed in claim 1 for improving glucose -lipid metabolism disorder and vascular function, its feature exist In the process for preparing medicine of the improvement glucose -lipid metabolism disorder and vascular function comprises the following steps：Step 1, red rooted salvia is ground into fine powder, crosses the mesh sieve of 20 mesh~60；Step 2, ethanol solution is added, is soaked at less than 5 DEG C, ultrasonic extraction, extraction process temperature is less than 25 DEG C；Step 3, extract solution centrifugation, filtering, ethanol is recovered under reduced pressure to extract solution without alcohol taste, extract solution concentration, filtering；Step 4, extract solution is clarified, first passes through macroporous resin enrichment, then carry out column chromatography purifying, obtained purity and be more than 90% phenolic acid B fraction；Freeze-drying, obtains root of red-rooted salvia phenolic acid B extract.
- 4. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 3, it is characterised in that institute Fine powder in step 1 is stated to form using biochemical method or Mechanical Crushing.
- 5. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 3, it is characterised in that institute State in step 2, the ethanol solution amount of addition is 8 times~10 times volumes of medicinal material, and the ethanol solution is percent by volume 40% ~80% ethanol solution.
- 6. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 3, it is characterised in that institute State in step 2, soak time is 12 hours, and the ultrasonic extraction time is 20min~60min.
- 7. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 3, it is characterised in that institute Extract solution in step 3 is stated except alcohol concentration is concentrated using reduced vacuum, during concentration, extract solution temperature is less than 60 DEG C.
- 8. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 3, it is characterised in that institute State in step 4, macroreticular resin is used by the macroporous resin enrichment：Nonpolar or low pole macroreticular resin；The non-pole Property or low pole macroreticular resin are AB-8, D-101 or HPD750.
- 9. improve the process for preparing medicine of glucose -lipid metabolism disorder and vascular function as claimed in claim 1, it is characterised in that institute State in step 4, the chromatographic column is dynamic axial pressurization chromatographic column, and chromatographic column filler is silica gel or polyamide；The step 4 is specially：Extract solution is clarified, first passes through macroporous resin enrichment, then carries out column chromatography purifying, successively Removal of impurities is eluted with the ethanol solution of the water of 1 times~3 times column volumes, 3 times~5 times column volumes 20%~30%, then again with 40% ~50% ethanol solution elution root of red-rooted salvia phenolic acid B, merges the eluent containing root of red-rooted salvia phenolic acid B, after concentrated drying, it is big to obtain purity In 90% root of red-rooted salvia phenolic acid B dry powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109851631A (en) * | 2017-11-30 | 2019-06-07 | 江苏曼氏生物科技股份有限公司 | A kind of isolation and purification method of lecithin in high purity |
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| CN102351819A (en) * | 2011-10-27 | 2012-02-15 | 广州汉方现代中药研究开发有限公司 | Extraction, purification and preparation method of high-purity salvianolic acid B |
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2017
- 2017-07-12 CN CN201710563564.XA patent/CN107349196A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102351819A (en) * | 2011-10-27 | 2012-02-15 | 广州汉方现代中药研究开发有限公司 | Extraction, purification and preparation method of high-purity salvianolic acid B |
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| 周才杰等: "丹酚酸B改善2型糖尿病大鼠糖脂代谢及胰岛素抵抗的实验研究", 《中国实验方剂学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109851631A (en) * | 2017-11-30 | 2019-06-07 | 江苏曼氏生物科技股份有限公司 | A kind of isolation and purification method of lecithin in high purity |
| CN109851631B (en) * | 2017-11-30 | 2021-11-30 | 江苏曼氏生物科技股份有限公司 | Separation and purification method of high-purity phosphatidylcholine |
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Application publication date: 20171117 |
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