CN107347637A - Suspension culture of Aquilaria sinensis method for tissue culture for large-scale plantation - Google Patents
Suspension culture of Aquilaria sinensis method for tissue culture for large-scale plantation Download PDFInfo
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- CN107347637A CN107347637A CN201710493948.9A CN201710493948A CN107347637A CN 107347637 A CN107347637 A CN 107347637A CN 201710493948 A CN201710493948 A CN 201710493948A CN 107347637 A CN107347637 A CN 107347637A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of suspension culture of Aquilaria sinensis method for tissue culture for large-scale plantation, including Aseptic seedling culture, bud induction and bud propagation, Rooting and hardening-off culture and acclimatization and transplantses.Have the beneficial effect that：The inventive method cultivate suspension culture of Aquilaria sinensis seedling time it is short, obtained suspension culture of Aquilaria sinensis seedling diseases poison less, stable hereditary property, obtained suspension culture of Aquilaria sinensis branch is thicker, and wind resistance is stronger, vitality compared with prior art tissue cultures cultivate suspension culture of Aquilaria sinensis it is more vigorous, survival rate is higher.More root restriction is formed under auxin effect without offspring, allows root to extend simultaneously normal growth under the inhibitory action without auxin, so as to solve the contradiction between the generation of root restriction and root elongation, and reduces calli induction.Using the method that indirect induction suspension culture of Aquilaria sinensis explant is taken root stage by stage, not only rooting rate is higher, and rooted seedling base portion is good without callus, root growth.
Description
Technical field
The present invention relates to field of plant tissue culture technique, specifically a kind of suspension culture of Aquilaria sinensis tissue for large-scale plantation is trained
The method of supporting.
Background technology
Suspension culture of Aquilaria sinensis (Aquilaria sinensis), also known as tabernaemontanus bulrush perfume, Nv Erxiang, buta-buta, it is Thymelaeceae
(Thmelaceae) plant.Suspension culture of Aquilaria sinensis tree is the xylophyta of perennial evergreen, is spices and medicinal plant, and China is unique
The plant resources of agalloch eaglewood can be produced.Wild resource is mainly distributed on the provinces and cities such as Guangxi province, Hainan Province and Fujian Province, there is small part
It is distributed in the areas such as Xishuangbanna and the Simao in Yunnan.Suspension culture of Aquilaria sinensis tree is a kind of rare medicinal material, advanced spices, with the world
The increase of market demand quantity, its price also constantly go up.First-class agalloch eaglewood price of medicinal material per kilogram is up on ten thousand yuan, makes it
Commercially supply falls short of demand.Suspension culture of Aquilaria sinensis its acrid flavour, hardship, warm-natured, tool receives gas and relievings asthma, has the effect of analgesic antiemetic, is mainly used in controlling
Vexed pain that treatment gastrofrigid vomiting, kidney deficiency are panted, chest and abdomen rise.Thus the excessive tree felling that brings and perfume (or spice) is adopted so that buta-buta resource faces
Extinction, suspension culture of Aquilaria sinensis tree are cited as national Precious, Rare, Endangered three-level protective plant for 1987, and secondly 1999 are again state by state approval
Business institute two level Top-rated protected wild plants.
Suspension culture of Aquilaria sinensis tree is to belong to nature wild tree species, and modes of reproduction mainly passes through seminal propagation, but wild adult at present
Elite stand it is extremely rare, substantial amounts of seed can not be produced, cause to be widely applied plantation more difficult;And seminal propagation
Seedling is easier to produce variation, is difficult to ensure and stays breeding advantage.
Plant Tissue Breeding be it is a kind of the part cell or tissue of plant is separated with parent, under suitable condition plus
With culture, the technology for growing, developing, break up and breeding is allowed them to.Principle is the totipotency differentiation potency from plant cell
Certain a kind of cell in power, that is, plant, can independently develop and break up as complete plant adult.Plant tissue
Culture can turn out substantial amounts of plant with a small amount of parent, and this makes Plant Tissue Breeding have many purposes, such as basis is planted
Thing and genetics research, and breeding and kind reservation agriculturally.
Prior art such as Authorization Notice No. is the B of CN 104686341 Chinese invention patent, discloses a kind of suspension culture of Aquilaria sinensis
Tissue culture technique, it is related to suspension culture of Aquilaria sinensis（Aquilaria sinensis）The nursery of high quality seedling is obtained by rapid propagation in vitro technology
Method.This method sterilizes, inducing clumping bud, culture of rootage, hardening are moved using suspension culture of Aquilaria sinensis stem with bud as explant by explant
The processes such as cultivation accelerate popularization and the development of resources profit of suspension culture of Aquilaria sinensis breeding so as to establish suspension culture of Aquilaria sinensis tissue-culturing quick-propagation system
With having important practical significance.But the amount of Multiple Buds is few in the above method, the Multiple Buds color grown is partially yellow, and vitality is not prosperous
Contain.
The content of the invention
It is an object of the invention to provide a kind of amount of Multiple Buds is more, obtained bud-leaf of growing thickly is big and green, and vitality is vigorous
The suspension culture of Aquilaria sinensis method for tissue culture for large-scale plantation.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Whitewood for large-scale plantation
Fragrant method for tissue culture, including Aseptic seedling culture, bud induction and bud propagation, Rooting and hardening-off culture and acclimatization and transplantses.Aseptic seedling is trained
Supporting step includes suspension culture of Aquilaria sinensis seed embryo first using sterile water wash, then is rinsed with 70 ~ 80% alcohol, is placed into after sterile water wash
Soaked in 10 ~ 18% sodium hypochlorite, then with sterile water wash, and be put into seed germination medium.Seed germination medium is to add
0.7 ~ 1.5mg/L GA is added3MS culture mediums.Seed disinfection is easier to, and pollution rate is low, directly takes suspension culture of Aquilaria sinensis axillary bud to be used as outer
Implant sterilization is more difficult, and pollution rate is high.
Bud induces and bud amplification step includes removal and removes seedling cotyledon, leaves terminal bud and is transferred to 0.7 ~ 1.5mg/ of addition
L6-BA, 0.07 ~ 0.15mg/LNAA, the MS culture mediums of 0.4 ~ 0.6mg/LKT and bioactive peptide, i.e., train in bud inducement cultivation base
Support, form Multiple Buds.The terminal bud for choosing aseptic seedling shortens the induction time of Multiple Buds for explant；Cotyledon and the stem without bud
Section can only induce substantial amounts of callus, so explant elects germ free apical bud as.The amount for the Multiple Buds that the above method is cultivated
More, obtained bud-leaf of growing thickly is big and green, and vitality is vigorous.
The amino acid sequence of bioactive peptide is SCASVCKSHRARRCGSVFRCYCRCLRC.Above-mentioned bioactive peptide can be obvious
The growth rate of cell after breaking up is improved, the amount of obtained Multiple Buds is more, and leaf is big and green, and vitality is vigorous.
Bud induce and bud amplification step include by Multiple Buds be transferred to 0.04 ~ 0.06mg/LBAP of addition, 27 ~ 34g/L sucrose,
Squamous subculture in the MS culture mediums of 0.07 ~ 0.14g/L inositols and 6 ~ 9g/L agar, obtains no offspring clump, by seedling clump without offspring
Cut from base portion, and be cut into the segment of 0.4 ~ 0.7cm length, then remaining seedling clump after segment and cutting is transferred to bud induction training respectively
Support and cultivated in base, form Multiple Buds, repeat the above steps to obtain substantial amounts of Multiple Buds and without offspring.
Rooting and hardening-off culture step is that first will be transferred to addition 0.8 ~ 1.5mg/LIBA and 0.4 ~ 0.6mg/LNAA without offspring
1/2MS culture mediums in cultivate 1 ~ 3d, then be transferred in the 1/2MS culture mediums of no auxin and lure root to produce indirectly.Without offspring in life
More root restriction is formed under long element effect, allows root to extend simultaneously normal growth under the inhibitory action without auxin, so as to solve
Contradiction between the generation of root restriction and root elongation, and reduce calli induction.Whitewood is induced using the above method
Fragrant explant is taken root, and not only rooting rate is higher, and rooted seedling base portion is good without callus, root growth.
The test tube seedling length of acclimatization and transplantses step including under growth root to 3 ~ 5cm it is high when, test tube seedling is put scatter before window 3 at light ~
5d opens sealed membrane 2 ~ 3d of hardening again, and test tube seedling is then taken out from bottle, and the nutrient solution of adhesion root system is cleaned with clear water, is transferred to
In the matrix of perlite, sufficient root water is drenched, it is windproof to put narrow meshed polybag moisturizing, plastic sheath is removed after 20 ~ 30d, afterwards
Every 1 ~ 2d water sprays once.The cuticula of test tube seedling blade surface has been formed, and is effective against low intensive light irradiation and is subtracted
Few moisture loss, but resisting stress is weak, and the above method can improve suspension culture of Aquilaria sinensis seedling straw stiffness, strengthen and resist sudden ability.
Compared with prior art, the advantage of the invention is that：
1. the inventive method cultivate suspension culture of Aquilaria sinensis seedling time it is short, obtained suspension culture of Aquilaria sinensis seedling diseases poison less, stable hereditary property, obtain
Suspension culture of Aquilaria sinensis branch is thicker, and wind resistance is stronger, and the vitality suspension culture of Aquilaria sinensis that tissue cultures are cultivated compared with prior art is more vigorous, survives
Rate is higher.
2. the activity that amino acid sequence is SCASVCKSHRARRCGSVFRCYCRCLRC is added in bud differential medium
Small peptide.Above-mentioned bioactive peptide can significantly improve the growth rate of cell after differentiation, and the amount of Multiple Buds is more, and obtained bud-leaf of growing thickly is big
And it is green, vitality is vigorous.
3. forming more root restriction under auxin effect without offspring, root is allowed to stretch under the inhibitory action without auxin
Long and normal growth, so as to solve the contradiction between the generation of root restriction and root elongation, and reduce calli induction.
Using the method that indirect induction suspension culture of Aquilaria sinensis explant is taken root stage by stage, not only rooting rate is higher, and rooted seedling base portion is without callus
Tissue, root growth are good.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
For the suspension culture of Aquilaria sinensis method for tissue culture of large-scale plantation, including the induction of Aseptic seedling culture, bud and bud propagation, strong plantlets and rootage
Culture and acclimatization and transplantses.Aseptic seedling culture step includes suspension culture of Aquilaria sinensis seed embryo first using sterile water wash, then is floated with 75% alcohol
Wash, place into 12% sodium hypochlorite and soak after sterile water wash, then with sterile water wash, and be put into seed germination medium.
Seed germination medium is the GA that with the addition of 1.2mg/L3MS culture mediums.Seed disinfection is easier to, and pollution rate is low, is directly taken white
Banksia rose axillary bud sterilizes more difficult, pollution rate height as explant.
Bud induces and bud amplification step includes removal and removes seedling cotyledon, leaves terminal bud and is transferred to addition 1.2mg/L6-
Cultivated in BA, 1.2mg/LNAA, 0.5mg/LKT and bioactive peptide MS culture mediums, i.e. bud inducement cultivation base, form Multiple Buds.
The terminal bud for choosing aseptic seedling shortens the induction time of Multiple Buds for explant；Cotyledon and stem section without bud can only induce greatly
The callus of amount, so explant elects germ free apical bud as.The amount for the Multiple Buds that the above method is cultivated is more, obtained Multiple Buds
Leaf is big and green, and vitality is vigorous.
The amino acid sequence of bioactive peptide is SCASVCKSHRARRCGSVFRCYCRCLRC.Above-mentioned bioactive peptide can be obvious
The growth rate of cell after breaking up is improved, the amount of obtained Multiple Buds is more, and leaf is big and green, and vitality is vigorous.
Bud induces and bud amplification step includes Multiple Buds being transferred to addition 0.05mg/LBAP, 31g/L sucrose, 1.2g/L fleshes
Squamous subculture in the MS culture mediums of alcohol and 8g/L agar, obtains no offspring clump, and seedling clump is cut without offspring from base portion, and is cut into
The segment of 0.5cm length, then remaining seedling clump after segment and cutting is transferred in bud inducement cultivation base respectively and cultivated, formation is grown thickly
Bud, repeat the above steps to obtain substantial amounts of Multiple Buds and without offspring.
Rooting and hardening-off culture step adds 1.2mg/LIBA and 0.5mg/LNAA 1/2MS trainings will to be first transferred to without offspring
Support in base and cultivate 2d, then be transferred in the 1/2MS culture mediums of no auxin and lure root to produce indirectly.Without offspring auxin effect under shape
Into more root restriction, root is allowed to extend simultaneously normal growth under the inhibitory action without auxin, so as to solve the hair of root restriction
Contradiction between the elongation of raw and root, and reduce calli induction.Taken root using above method induction suspension culture of Aquilaria sinensis explant,
Not only rooting rate is higher, and rooted seedling base portion is good without callus, root growth.
The test tube seedling length of acclimatization and transplantses step including under growth root to 3 ~ 5cm it is high when, scatter 4d at light before test tube seedling is put into window,
Sealed membrane hardening 2d is opened again, test tube seedling is then taken out from bottle, and the nutrient solution of adhesion root system is cleaned with clear water, is transferred to pearl
In the matrix of rock, drench sufficient root water, it is windproof to put narrow meshed polybag moisturizing, and plastic sheath is removed after 2,5d, afterwards every 1 ~
2d sprays water once.The cuticula of test tube seedling blade surface has been formed, and is effective against low intensive light irradiation and is reduced moisture
Scatter and disappear, but resisting stress is weak, and the above method can improve suspension culture of Aquilaria sinensis seedling straw stiffness, strengthen and resist sudden ability.
Embodiment 2：
For the suspension culture of Aquilaria sinensis method for tissue culture of large-scale plantation, comprise the following steps：
1）Aseptic seedling culture：Suspension culture of Aquilaria sinensis seed embryo is first used into sterile water wash, then rinsed with 75% alcohol, after sterile water wash again
It is put into 15% sodium hypochlorite and soaks, then with sterile water wash, and be put into seed germination medium.Seed germination medium is
It with the addition of 1mg/L GA3MS culture mediums；
2）Bud induces and bud propagation：Seedling cotyledon is removed in removal, leaves terminal bud and is transferred to addition 1.0mg/L6-BA, 1.0mg/
Cultivated in LNAA, 0.5mg/LKT and bioactive peptide MS culture mediums, i.e. bud inducement cultivation base, form Multiple Buds.Bioactive peptide
Amino acid sequence is SCASVCKSHRARRCGSVFRCYCRCLRC.Multiple Buds are transferred to addition 0.05mg/LBAP, 30g/L sugarcane
Sugar, 1.0g/L inositols and 8g/L agar MS culture mediums in squamous subculture, no offspring clump is obtained, by seedling clump without offspring from base portion
Cutting, and the segment of 0.6cm length is cut into, then remaining seedling clump after segment and cutting is transferred in bud inducement cultivation base respectively and trained
Support, form Multiple Buds, repeat the above steps to obtain substantial amounts of Multiple Buds and without offspring；
3）Rooting and hardening-off culture：It will be first transferred to without offspring in addition 1.0mg/LIBA and 0.5mg/LNAA 1/2MS culture mediums
2d is cultivated, then is transferred in the 1/2MS culture mediums of no auxin and lures root to produce indirectly；
4）Acclimatization and transplantses：The test tube seedling length of under growth root to 3 ~ 5cm it is high when, test tube seedling is put 4d at light is scattered before window, then open envelope
Membrana oralis hardening 2d, then takes out test tube seedling from bottle, and the nutrient solution of adhesion root system is cleaned with clear water, is transferred to the matrix of perlite
In, sufficient root water is drenched, it is windproof to put narrow meshed polybag moisturizing, and plastic sheath is removed after 2,5d, sprays water one every 1 ~ 2d afterwards
It is secondary.
Embodiment 3：
For the suspension culture of Aquilaria sinensis method for tissue culture of large-scale plantation, comprise the following steps：
1）Aseptic seedling culture：Suspension culture of Aquilaria sinensis seed embryo is first used into sterile water wash, then rinsed with 80% alcohol, after sterile water wash again
It is put into 13% sodium hypochlorite and soaks, then with sterile water wash, and be put into seed germination medium.Seed germination medium is
It with the addition of 1.1mg/L GA3MS culture mediums；
2）Bud induces and bud propagation：Seedling cotyledon is removed in removal, leaves terminal bud and is transferred to addition 1.1mg/L6-BA, 1.1mg/
Cultivated in LNAA, 0.6mg/LKT and bioactive peptide MS culture mediums, i.e. bud inducement cultivation base, form Multiple Buds.Bioactive peptide
Amino acid sequence is SCASVCKSHRARRCGSVFRCYCRCLRC.Multiple Buds are transferred to addition 0.06mg/LBAP, 32g/L sugarcane
Sugar, 1.1g/L inositols and 7g/L agar MS culture mediums in squamous subculture, no offspring clump is obtained, by seedling clump without offspring from base portion
Cutting, and the segment of 0.6cm length is cut into, then remaining seedling clump after segment and cutting is transferred in bud inducement cultivation base respectively and trained
Support, form Multiple Buds, repeat the above steps to obtain substantial amounts of Multiple Buds and without offspring；
3）Rooting and hardening-off culture：It will be first transferred to without offspring in addition 1.1mg/LIBA and 0.5mg/LNAA 1/2MS culture mediums
2d is cultivated, then is transferred in the 1/2MS culture mediums of no auxin and lures root to produce indirectly；
4）Acclimatization and transplantses：The test tube seedling length of under growth root to 3 ~ 5cm it is high when, test tube seedling is put 4d at light is scattered before window, then open envelope
Membrana oralis hardening 2d, then takes out test tube seedling from bottle, and the nutrient solution of adhesion root system is cleaned with clear water, is transferred to the matrix of perlite
In, sufficient root water is drenched, it is windproof to put narrow meshed polybag moisturizing, and plastic sheath is removed after 2,5d, sprays water one every 1 ~ 2d afterwards
It is secondary.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County Mei Ze bio tech ltd
<120>Suspension culture of Aquilaria sinensis method for tissue culture for large-scale plantation
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 27
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Val Cys Lys Ser His Arg Ala Arg Arg Cys Gly Ser
1 5 10 15
Val Phe Arg Cys Tyr Cys Arg Cys Leu Arg Cys
20 25
Claims (7)
1. for large-scale plantation suspension culture of Aquilaria sinensis method for tissue culture, including Aseptic seedling culture, bud induction and bud propagation, take root it is strong
Seedling culture and acclimatization and transplantses, it is characterised in that：Described Aseptic seedling culture step includes suspension culture of Aquilaria sinensis seed embryo first using sterilized water
Cleaning, then rinsed with 70 ~ 80% alcohol, place into 10 ~ 18% sodium hypochlorite and soak after sterile water wash, then it is clear with sterilized water
Wash, and be put into seed germination medium.Seed disinfection is easier to, and pollution rate is low, directly takes suspension culture of Aquilaria sinensis axillary bud as explant
Sterilization is more difficult, and pollution rate is high；The terminal bud for choosing aseptic seedling shortens the induction time of Multiple Buds for explant；Cotyledon and without
The stem section of bud can only induce substantial amounts of callus, so explant elects germ free apical bud as.
2. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 1 for large-scale plantation, it is characterised in that：Described
Seed germination medium is the GA that with the addition of 0.7 ~ 1.5mg/L3MS culture mediums.
3. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 1 for large-scale plantation, it is characterised in that：Described
Bud induce and bud amplification step include remove seedling cotyledon is removed, leave terminal bud be transferred to 0.7 ~ 1.5mg/L6-BA of addition,
Cultivate, formed in 0.07 ~ 0.15mg/LNAA, 0.4 ~ 0.6mg/LKT and bioactive peptide MS culture mediums, i.e. bud inducement cultivation base
Multiple Buds.
4. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 3 for large-scale plantation, it is characterised in that：Described
The amino acid sequence of bioactive peptide is SCASVCKSHRARRCGSVFRCYCRCLRC.
5. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 1 for large-scale plantation, it is characterised in that：Described
Bud induce and bud amplification step include by Multiple Buds be transferred to 0.04 ~ 0.06mg/LBAP of addition, 27 ~ 34g/L sucrose, 0.07 ~
Squamous subculture in the MS culture mediums of 0.14g/L inositols and 6 ~ 9g/L agar, obtains no offspring clump, by seedling clump without offspring from base portion
Cutting, and the segment of 0.4 ~ 0.7cm length is cut into, then remaining seedling clump after segment and cutting is transferred in bud inducement cultivation base respectively
Culture, Multiple Buds are formed, repeat the above steps to obtain substantial amounts of Multiple Buds and without offspring.
6. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 1 for large-scale plantation, it is characterised in that：Described
Rooting and hardening-off culture step is that the 1/2MS that addition 0.8 ~ 1.5mg/LIBA and 0.4 ~ 0.6mg/LNAA will be first transferred to without offspring is trained
Support and 1 ~ 3d is cultivated in base, then be transferred in the 1/2MS culture mediums of no auxin and lure root to produce indirectly.Without offspring under auxin effect
More root restriction is formed, allows root to extend simultaneously normal growth under the inhibitory action without auxin, so as to solve root restriction
The contradiction between root elongation occurs, and reduces calli induction.Using above method induction suspension culture of Aquilaria sinensis explant life
Root, not only rooting rate is higher, and rooted seedling base portion is good without callus, root growth.
7. the suspension culture of Aquilaria sinensis method for tissue culture according to claim 1 for large-scale plantation, it is characterised in that：Described
The test tube seedling length of acclimatization and transplantses step including under growth root to 3 ~ 5cm it is high when, test tube seedling is put and scatters 3 ~ 5d at light before window and opens again
Sealed membrane 2 ~ 3d of hardening, then takes out test tube seedling from bottle, and the nutrient solution of adhesion root system is cleaned with clear water, is transferred to perlite
In matrix, sufficient root water is drenched, it is windproof to put narrow meshed polybag moisturizing, plastic sheath is removed after 20 ~ 30d, afterwards every 1 ~ 2d
Water spray is once.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841856A (en) * | 2018-06-19 | 2018-11-20 | 中国医学科学院药用植物研究所 | The hairy root induction of suspension culture of Aquilaria sinensis and its genetic transforming method |
| CN117502228A (en) * | 2023-10-19 | 2024-02-06 | 南京林业大学 | Establishment method of aquilaria sinensis tissue culture rapid propagation system |
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|---|---|---|---|---|
| CN104686341A (en) * | 2015-02-22 | 2015-06-10 | 梁仕华 | Tissue culture technique of aquilaria sinensis |
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2017
- 2017-06-26 CN CN201710493948.9A patent/CN107347637A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686341A (en) * | 2015-02-22 | 2015-06-10 | 梁仕华 | Tissue culture technique of aquilaria sinensis |
Non-Patent Citations (3)
| Title |
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| 叶勤法等: "白木香组织培养及快速繁殖", 《植物学通报》 * |
| 林妃等: "白木香组织培养技术及植株再生的研究", 《基因组学与应用生物学》 * |
| 葛胜娟: "《植物组织培养》", 30 September 2008, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841856A (en) * | 2018-06-19 | 2018-11-20 | 中国医学科学院药用植物研究所 | The hairy root induction of suspension culture of Aquilaria sinensis and its genetic transforming method |
| CN117502228A (en) * | 2023-10-19 | 2024-02-06 | 南京林业大学 | Establishment method of aquilaria sinensis tissue culture rapid propagation system |
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