CN107340290A - A kind of urine detection test paper for reacting fatigue level of human body and preparation method thereof - Google Patents
A kind of urine detection test paper for reacting fatigue level of human body and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to technical field of medical detection, a kind of more particularly to urine detection test paper for reacting fatigue level of human body and preparation method thereof, contain thiol protease, sodium dihydrogen phosphate, Water-soluble polythiophene, four water gold chlorides, sodium hydroxide and polyvinyl alcohol in the urine detection test paper, urine detection test paper Surface coating has chitosan/titanium dioxide composite membrane；Its preparation method is immersed filter paper in the mixed solution of solution A and solution B, again last layer chitosan/titanium dioxide composite membrane is covered in its surface, contain Water-soluble polythiophene, four water gold chlorides, sodium dihydrogen phosphate, sodium hydroxide in solution A, contain polyvinyl alcohol and thiol protease in solution B.The urine detection test paper that the preparation method of the present invention is prepared has stronger hydrophily, has absorption, osmosis to Urine proteins, easy to operate, detection sensitivity is higher.
Description
Technical field
The present invention relates to technical field of medical detection, more particularly to a kind of urine detection test paper for reacting fatigue level of human body
And preparation method thereof.
Background technology
Fatigue is that labourer due to the change of physiology and psychological condition, produces some or some organs are in labout process
To the natural depletion of whole body strength.Fatigue is a kind of physiological phenomenon, and a kind of psychological phenomena, is in essence
A kind of normal physiological protection mechanism of body.From biomedicine theoretically, work is the process of energy expenditure, and this process is held
Continuous central nervous system will produce inhibitory action to a certain extent, be exactly allocinesis nerve after central nervous system fatigue
The fatigue of system, reflecting the sensitivity of action reduces, and operating efficiency declines.Sense of fatigue is subjective experience of the people for fatigue,
And it is tired objective reaction that operating efficiency, which declines,.No matter in mental operation, manual operation, technical ability operation or man-machine system
The efficiency and health of people can all be affected because of fatigue.
Occur albumen in urine and be referred to as albuminuria, also referred to as Urine proteins.Protein content is little during normal person urinates in peace and quiet
(daily output ＜ 150mg), routine inspection method can not detect, therefore commonly referred to as feminine gender；And moving can arrange protein in urine
Output increase, easily detects to be positive, referred to as motility Urine proteins, motility Urine proteins belong to feature Urine proteins, typically existed
It can die away in 24h.After motion urinate in protein excretion because body functional state, exercise load it is different and different,
Therefore athletes ' body functional state or its adaptation situation can be evaluated according to urine albumin after exercise excretion and constituent.
For clear, timely, easily understanding sportsman degree of fatigue, and then sentific training can be carried out, typically taken
After motion and next day urina sanguinis does inspection to evaluate its fatigue and recovery extent.If protein content is higher in urina sanguinis, more than just
Constant value, it may be possible to the performance of fatigue or over training.At present, the detection method of Urine proteins is divided into a variety of, including sulfo group water
Poplar acid system, heating Acetic, dry chemistry test band method, nephelometry, dye binding method, chemical colorimetry.It is most in these methods to need
The professional qualification mechanism such as hospital laboratory, training laboratory, third party inspection center is wanted to operate completion, such detection efficiency
It is too low.Dry chemistry test therein band method operates the easiest, can utilize and visually observe the colored change of examination, then with on-gauge plate
Contrast draws corresponding numerical value, is the effective ways of quick detection urine protein content.But Urine proteins detection examination in existing urine
The detection sensitivity of paper is relatively low, and when content is little in urine, change colour especially Urine proteins unobvious, has a strong impact on testing result
Accuracy.
The content of the invention
In view of this, it is an object of the invention to provide a kind of urine detection test paper for reacting fatigue level of human body and its preparation
Method, the urine detection test paper that the preparation method is prepared have stronger hydrophily, to Urine proteins have stronger absorption,
Osmosis, easy to operate, detection sensitivity is higher.
The present invention solves above-mentioned technical problem by following technological means：
A kind of urine detection test paper for reacting fatigue level of human body, in the urine detection test paper containing thiol protease,
Sodium dihydrogen phosphate, Water-soluble polythiophene, four water gold chlorides, sodium hydroxide and polyvinyl alcohol, the urine detection test paper surface bag
It is covered with chitosan/titanium dioxide composite membrane.
Further, the urine detection test paper is in light yellow that the urine detection test paper surface is covered with 30 μm~100 μ m-thicks
Chitosan/titanium dioxide composite membrane.
The invention also discloses a kind of preparation method for the urine detection test paper for reacting fatigue level of human body, the preparation side
Method is that filter paper is immersed into volume ratio for 3:In 1 solution A, the mixed solution of solution B, then cover last layer shell in its surface and gather
Sugar/titanium dioxide composite film, 0.35g~0.85g Water-soluble polythiophenes, 0.35g~0.85g tetra- are contained in every liter of solution A
Water gold chloride, 16g~26g sodium dihydrogen phosphates, 2g~5g sodium hydroxides, contain 30g~45g polyethylene in every liter of solution B
Alcohol, 6.5g~13g thiol proteases.
Further, the preparation method comprises the following steps：
Configure solution A：Weigh Water-soluble polythiophene, four water gold chlorides, sodium dihydrogen phosphate and sodium hydroxide and be dissolved in deionization
Stirred in water, ultrasonic wave mixes, that is, obtains solution A.
Configure solution B：Load weighted polyvinyl alcohol is added in deionized water, heating is completely dissolved polyvinyl alcohol, cold
But to after normal temperature, thiol protease is added, is stirred and evenly mixed, that is, obtains solution B.
Immerse filter paper：Solution A and solution B are pressed 3:1 volume ratio is well mixed to obtain mixed solution, will be cut into rectangle
The filter paper of strip is totally immersed into mixed solution, is taken out after immersion 10min and is obtained the filter paper that solution is inhaled in leaching, is placed in 40 DEG C~48 DEG C
Baking oven in dry.
Overlay film is dried：The filter paper of drying is completely immersed in chitosan/titanium dioxide composite solution, taken out after soaking 30s,
Its surface is dried up with compressed air, then is completely immersed in 60s in chitosan/titanium dioxide composite solution and takes out, it is empty with compression
Gas dries up its surface, then immerses in chitosan/titanium dioxide composite solution and taken out after 180s, with compressed air by filter paper table
Ground drying, it is placed in drying in 40 DEG C~48 DEG C of baking oven, that is, obtains the urine inspection for having chitosan/titanium dioxide composite membrane to surface
Test paper.
Further, the preparation method of the chitosan/titanium dioxide composite solution is as follows：
Chitosan is dissolved in 1.00% glacial acetic acid solution, ultrasonic wave dissolving is opened, adds n-hydroxysuccinimide,
Ultrasonic wave mixing is continued on, EDC is added and stirs to being completely dissolved, add arginine, continues stirring 20h~24h and obtains instead
Liquid is answered, reaction solution is subjected to dialysis treatment, freeze-drying obtains, through the modified chitosan of arginine, weighing nano titanium oxide
Powder is dissolved in deionized water, and ultrasonic 20min obtains titanium dioxide emulsion, is added modified chitosan, is continued on
Ultrasonic echography, chitosan/titanium dioxide composite solution is made.
Further, the dialysis treatment is to immerse bag filter in distilled water fully to absorb water, and reaction solution is loaded into bag filter
It is middle to be dialysed 2 days with distilled water, first water during which was changed at interval of 8 hours, bag filter is finally put into 16% polyethylene glycol
24h is concentrated in solution.
Further, the crystalline structure of the nano titanium oxide is Detitanium-ore-type, and grain size is 20nm~50nm.
Further, the mass concentration of the titanium dioxide emulsion is 45%~55%.
Further, the mass ratio of the titanium dioxide and modified chitosan is 3:1.
Further, the ultrasound ginseng added in the chitosan/titanium dioxide composite solution preparation method after modification of chitosan
Number is frequency 25kHz~35kHz, and power 110W~150W, ultrasonic time is 20min~30min.
Because containing Water-soluble polythiophene, four water gold chlorides and thiol protease on the urine detection test paper of the present invention, do not make
With being in light yellow under state, it is contacted with Urine proteins occurs color change, with the increase color of protein concentration in urine
Gradually deepen, light yellow from most original is changed into lightpink, eventually becomes red, it compared with standard color card, you can sentence
Break and the concentration of Urine proteins in tested urine.The detection that the urine detection test paper is used for Urine proteins is easy to operate, quick, directly perceived.
Chitosan is the product of chitin deacetylation, is a kind of good biocompatibility, degradable, Nantural non-toxic linear
Polysaccharide boiomacromolecule.Contain free amino and multiple hydroxyls in chitosan, easily carry out hydroxylating, etherificate, acylation, alkane
Base and quaternised modified.By the method for graft modification, it is strong that processing is optionally grafted on chitosan macromolecular skeleton
Spend that high, hydrophily is strong, small molecule that have specific function, the performance of chitosan can be improved.It is modified through arginine
Chitosan has loose structure, and has suction-operated to Urine proteins, during for urine detection, can make the Urine proteins in urine
On chitosan/titanium dioxide composite membrane internally in the presence of filter paper chromogenic reaction occurs for quick adsorption, has higher inspection
Survey sensitivity.
Covered with one layer of chitosan/titanium dioxide composite membrane, the chitosan/titanium dioxide on the urine detection test paper of the present invention
Titanium compound film has ordered mesopore structure and larger specific surface area, and chitosan and the hydrophily of titanium dioxide after modification are made
Under, other impurity in urine and are trapped in outside film, so as to improve by Urine proteins that can be in quick adsorption, infiltration urine
The sensitivity of Urine proteins detection.Urine proteins are infiltrated on internal filter paper by chitosan/titanium dioxide composite membrane, and on filter paper
Water-soluble polythiophene combine after polythiophene structure can be caused to change it is defeated so that the optical property of polythiophene changes
Go out the optical signalling that can be detected, show as color and significant change occurs, be easy to observe.
Embodiment
Below with reference to specific embodiment, the present invention is described in detail：
Chitosan/titanium dioxide composite solution prepares embodiment one
Weigh 50g chitosans to be dissolved in the glacial acetic acid solution that 4.0L mass concentrations are 1.00%, opening ultrasonic wave gathers shell
Sugar is completely dissolved, and adds 36gN- HOSu NHSs, is continued on ultrasonic wave mixing, is added 45g1- (3- dimethylaminos
Propyl group) -3- ethyl carbodiimides are stirred to being completely dissolved, and add 50g arginine, and continue to stir 20h obtaining reaction solution, will react
Liquid carries out dialysis treatment, first immerses in distilled water bag filter before dialysis treatment and fully absorbs water, then reaction solution is loaded into bag filter
It is middle to be dialysed 2 days with distilled water, first water during which was changed at interval of 8 hours, bag filter is finally put into 16% polyethylene glycol
After 24h being concentrated in solution, the precooling half an hour under -6 DEG C~-3 DEG C of temperature conditionss, then be transferred in freeze drier and freeze
It is dried to obtain through the modified chitosan of arginine.
Weigh 15g nano titanium oxide powders to be dissolved in deionized water, it is molten to obtain the titanium dioxide that mass concentration is 45%
Liquid, ultrasonic 20min obtains titanium dioxide emulsion under normal temperature, adds the modified chitosans of 5g, is 45 DEG C in temperature, frequency
For 25kHz, power is ultrasonic disperse 20min under conditions of 110W, and chitosan/titanium dioxide composite solution is made.
Chitosan/titanium dioxide composite solution prepares embodiment two
Weigh 70g chitosans to be dissolved in the glacial acetic acid solution that 6.5L mass concentrations are 1.00%, opening ultrasonic wave gathers shell
Sugar is completely dissolved, and adds 50gN- HOSu NHSs, is continued on ultrasonic wave mixing, is added 60g1- (3- dimethylaminos
Propyl group) -3- ethyl carbodiimides are stirred to being completely dissolved, and add 38g arginine, and continue to stir 24h obtaining reaction solution, will react
Liquid carries out dialysis treatment, first immerses in distilled water bag filter before dialysis treatment and fully absorbs water, then reaction solution is loaded into bag filter
It is middle to be dialysed 2 days with distilled water, first water during which was changed at interval of 8 hours, bag filter is finally put into 16% polyethylene glycol
After 24h being concentrated in solution, the precooling half an hour under -6 DEG C~-3 DEG C of temperature conditionss, then be transferred in freeze drier and freeze
It is dried to obtain through the modified chitosan of arginine.
Weigh 15g nano titanium oxide powders to be dissolved in deionized water, it is molten to obtain the titanium dioxide that mass concentration is 55%
Liquid, ultrasonic 20min obtains titanium dioxide emulsion under normal temperature, adds the modified chitosans of 5g, is 40 DEG C in temperature, frequency
For 30kHz, power is ultrasonic disperse 25min under conditions of 150W, and chitosan/titanium dioxide composite solution is made.
Chitosan/titanium dioxide composite solution prepares embodiment three
Weigh 60g chitosans to be dissolved in the glacial acetic acid solution that 5.0L mass concentrations are 1.00%, opening ultrasonic wave gathers shell
Sugar is completely dissolved, and adds 40gN- HOSu NHSs, is continued on ultrasonic wave mixing, is added 46g1- (3- dimethylaminos
Propyl group) -3- ethyl carbodiimides are stirred to being completely dissolved, and add 25g arginine, and continue to stir 22h obtaining reaction solution, will react
Liquid carries out dialysis treatment, first immerses in distilled water bag filter before dialysis treatment and fully absorbs water, then reaction solution is loaded into bag filter
It is middle to be dialysed 2 days with distilled water, first water during which was changed at interval of 8 hours, bag filter is finally put into 16% polyethylene glycol
After 24h being concentrated in solution, the precooling half an hour under -6 DEG C~-3 DEG C of temperature conditionss, then be transferred in freeze drier and freeze
It is dried to obtain through the modified chitosan of arginine.
Weigh 15g nano titanium oxide powders to be dissolved in deionized water, it is molten to obtain the titanium dioxide that mass concentration is 50%
Liquid, ultrasonic 20min obtains titanium dioxide emulsion under normal temperature, adds the modified chitosans of 5g, is 40 DEG C in temperature, frequency
For 35kHz, power is ultrasonic disperse 30min under conditions of 130W, and chitosan/titanium dioxide composite solution is made.
The crystalline structure of the nano titanium oxide used in embodiment one, embodiment two and embodiment three is Detitanium-ore-type,
Grain size is 20nm~50nm, and the preparation method of the nano titanium oxide is as follows：By titanium tetrachloride add absolute ethyl alcohol in
60 DEG C of stirring 8h, are prepared precursor solution, precursor solution and hydrochloric acid, zinc salt are mixed, H in obtained mixed solution+/
Ti=4, Zn/Ti=0.45, mixed solution is flowed back 20h at 100 DEG C, obtains sediment, filtered, after being washed with ethanol, done
Dry crushing, it is transferred in Muffle furnace and is warming up to 5 DEG C per minute of programming rate at 650 DEG C after preservation 3h, cooling obtains anatase
Type nano titanium oxide.
Urine detection test paper prepares example IV
Weigh 0.35g Water-soluble polythiophenes, the water gold chlorides of 0.45g tetra-, 16g sodium dihydrogen phosphates and 2g sodium hydroxides and be dissolved in 1L
Stirred in deionized water, open ultrasonic wave and be uniformly mixed so as to obtain solution A；Weigh 30g polyvinyl alcohol to add in 1L deionized waters, heating makes
Polyvinyl alcohol is completely dissolved, and after being cooled to normal temperature, is added 6.5g thiol proteases, is stirred and evenly mixed to obtain solution B；Take 600ml
Solution A and 200ml solution Bs, which are well mixed, obtains mixed solution, and the filter paper for being cut into rectangle strip is totally immersed into mixed solution
In, taken out after immersion 10min and obtain the filter paper that solution is inhaled in leaching, be placed in 48 DEG C of baking oven and dry；The filter paper of drying is soaked completely
In the chitosan/titanium dioxide composite solution for entering the preparation of embodiment one, taken out after soaking 30s, with compressed air by its surface
Drying, then be completely immersed in 60s in chitosan/titanium dioxide composite solution and take out, its surface is dried up with compressed air, then soaks
Enter in chitosan/titanium dioxide composite solution and taken out after 180s, its surface is dried up with compressed air, is placed in 48 DEG C of baking
Dried in case, that is, obtain the urine detection test paper for having chitosan/titanium dioxide composite membrane to surface.The urine of the present embodiment after testing
Chitosan/titanium dioxide composite film thickness on Test paper is 50 μm~100 μm.
Urine detection test paper prepares embodiment five
Weigh 0.85g Water-soluble polythiophenes, the water gold chlorides of 0.85g tetra-, 26g sodium dihydrogen phosphates and 5g sodium hydroxides and be dissolved in 1L
Stirred in deionized water, open ultrasonic wave and be uniformly mixed so as to obtain solution A；Weigh 40g polyvinyl alcohol to add in 1L deionized waters, heating makes
Polyvinyl alcohol is completely dissolved, and after being cooled to normal temperature, is added 10g thiol proteases, is stirred and evenly mixed to obtain solution B；Take 300ml molten
Liquid A and 100ml solution B are well mixed to obtain mixed solution, and the filter paper for being cut into rectangle strip is totally immersed into mixed solution,
Taken out after immersion 10min and obtain the filter paper that solution is inhaled in leaching, be placed in 44 DEG C of baking oven and dry；The filter paper of drying is completely immersed in reality
In the chitosan/titanium dioxide composite solution for applying the preparation of example one, take out after soaking 30s, dried up its surface with compressed air,
60s in chitosan/titanium dioxide composite solution is completely immersed in again to take out, and is dried up its surface with compressed air, then immerse shell
Taken out in glycan/titanium dioxide composite solution after 180s, its surface is dried up with compressed air, is placed in 44 DEG C of baking oven
Drying, that is, obtain the urine detection test paper for having chitosan/titanium dioxide composite membrane to surface.The urine detection of the present embodiment after testing
Chitosan/titanium dioxide composite film thickness on test paper is 30 μm~100 μm.
Urine detection test paper prepares embodiment six
Weigh 0.5g Water-soluble polythiophenes, the water gold chlorides of 0.65g tetra-, 20g sodium dihydrogen phosphates and 3g sodium hydroxides and be dissolved in 1L
Stirred in deionized water, open ultrasonic wave and be uniformly mixed so as to obtain solution A；Weigh 45g polyvinyl alcohol to add in 1L deionized waters, heating makes
Polyvinyl alcohol is completely dissolved, and after being cooled to normal temperature, is added 13g thiol proteases, is stirred and evenly mixed to obtain solution B；Take 600ml molten
Liquid A and 200ml solution B are well mixed to obtain mixed solution, and the filter paper for being cut into rectangle strip is totally immersed into mixed solution,
Taken out after immersion 10min and obtain the filter paper that solution is inhaled in leaching, be placed in 40 DEG C of baking oven and dry；The filter paper of drying is completely immersed in reality
In the chitosan/titanium dioxide composite solution for applying the preparation of example two, take out after soaking 30s, dried up its surface with compressed air,
60s in chitosan/titanium dioxide composite solution is completely immersed in again to take out, and is dried up its surface with compressed air, then immerse shell
Taken out in glycan/titanium dioxide composite solution after 180s, its surface is dried up with compressed air, is placed in 40 DEG C of baking oven
Drying, that is, obtain the urine detection test paper for having chitosan/titanium dioxide composite membrane to surface.The urine detection of the present embodiment after testing
Chitosan/titanium dioxide composite film thickness on test paper is 30 μm~80 μm.
Urine detection test paper prepares embodiment seven
Weigh 0.65g Water-soluble polythiophenes, the water gold chlorides of 0.70g tetra-, 22g sodium dihydrogen phosphates and 3g sodium hydroxides and be dissolved in 1L
Stirred in deionized water, open ultrasonic wave and be uniformly mixed so as to obtain solution A；Weigh 41g polyvinyl alcohol to add in 1L deionized waters, heating makes
Polyvinyl alcohol is completely dissolved, and after being cooled to normal temperature, is added 12g thiol proteases, is stirred and evenly mixed to obtain solution B；Take 600ml molten
Liquid A and 200ml solution B are well mixed to obtain mixed solution, and the filter paper for being cut into rectangle strip is totally immersed into mixed solution,
Taken out after immersion 10min and obtain the filter paper that solution is inhaled in leaching, be placed in 48 DEG C of baking oven and dry；The filter paper of drying is completely immersed in reality
In the chitosan/titanium dioxide composite solution for applying the preparation of example three, take out after soaking 30s, dried up its surface with compressed air,
60s in chitosan/titanium dioxide composite solution is completely immersed in again to take out, and is dried up its surface with compressed air, then immerse shell
Taken out in glycan/titanium dioxide composite solution after 180s, its surface is dried up with compressed air, is placed in 48 DEG C of baking oven
Drying, that is, obtain the urine detection test paper for having chitosan/titanium dioxide composite membrane to surface.The urine detection of the present embodiment after testing
Chitosan/titanium dioxide composite film thickness on test paper is 40 μm~70 μm.
The making of standard color card：Accurately weigh bovine serum albumin to be dissolved in distilled water, configure a series of containing for Exact concentrations
The solution of bovine serum albumin, the concentration of bovine serum albumin choose example IV to seven urines being prepared from 0 to 2.0mg/mL
Test paper immerses in the bovine serum albumen solution of various concentration respectively, is taken out after soaking 8s, spectral luminosity is used after keeping flat 5min
Meter or photoelectric integration colour photometer measure colourity, then by concentration order from small to large by corresponding color apply with art paper,
Obtain standard color card.
The application method of the urine detection test paper of the present invention is to immerse test paper in freshly voided urine, takes out, keeps flat after 8s
After 5min, compared with corresponding standard color card, you can judge the concentration of protein in tested urine.The urine detection test paper
For the detection simple operation of Urine proteins, it is quick on the draw, sentencing for fatigue level of human body can be used for according to the change in concentration of detection
It is fixed, if larger growth is presented in urinary protein concentrations in urine, more than normal value, then it is determined as that human body is in fatigue state.
The above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferred embodiment to this hair
It is bright to be described in detail, it will be understood by those within the art that, technical scheme can be modified
Or equivalent substitution, without departing from the objective and scope of technical solution of the present invention, it all should cover the claim in the present invention
Among scope.The present invention be not described in detail technology, shape, construction part be known technology.
Claims (10)
1. a kind of urine detection test paper for reacting fatigue level of human body, it is characterised in that contain mercapto in the urine detection test paper
Base protease, sodium dihydrogen phosphate, Water-soluble polythiophene, four water gold chlorides, sodium hydroxide and polyvinyl alcohol, the urine detection examination
Paper Surface coating has chitosan/titanium dioxide composite membrane.
2. a kind of urine detection test paper for reacting fatigue level of human body according to claim 1, wherein, the urine detection
Test paper is in light yellow, and the urine detection test paper surface is covered with the chitosan/titanium dioxide composite membrane of 30 μm~100 μ m-thicks.
3. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 2, its feature
It is, the preparation method is that filter paper is immersed into volume ratio for 3:In 1 solution A, the mixed solution of solution B, then in filter paper table
Last layer chitosan/titanium dioxide composite membrane is covered in face, in every liter of solution A containing 0.35g~0.85g Water-soluble polythiophenes,
The water gold chlorides of 0.35g~0.85g tetra-, 16g~26g sodium dihydrogen phosphates, 2g~5g sodium hydroxides, contain in every liter of solution B
30g~45g polyvinyl alcohol, 6.5g~13g thiol proteases.
4. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 3, wherein, institute
Preparation method is stated to comprise the following steps：
Configure solution A：Water-soluble polythiophene, four water gold chlorides, sodium dihydrogen phosphate and sodium hydroxide is weighed to be dissolved in deionized water
Stirring, ultrasonic wave mix, that is, obtain solution A；
Configure solution B：Load weighted polyvinyl alcohol is added in deionized water, heating is completely dissolved polyvinyl alcohol, is cooled to
After normal temperature, thiol protease is added, is stirred and evenly mixed, that is, obtains solution B；
Immerse filter paper：Solution A and solution B are pressed 3:1 volume ratio is well mixed to obtain mixed solution, will be cut into rectangle strip
Filter paper be totally immersed into mixed solution, soak to take out after 10min and obtain the filter paper that solution is inhaled in leaching, be placed in 40 DEG C~48 DEG C of baking
Dried in case；
Overlay film is dried：The filter paper of drying is completely immersed in chitosan/titanium dioxide composite solution, taken out after soaking 30s, with pressure
Contracting air dries up its surface, then is completely immersed in 60s in chitosan/titanium dioxide composite solution and takes out, will with compressed air
Its surface dries up, then immerses in chitosan/titanium dioxide composite solution and taken out after 180s, is blown its surface with compressed air
It is dry, it is placed in drying in 40 DEG C~48 DEG C of baking oven, that is, obtains the urine detection examination for having chitosan/titanium dioxide composite membrane to surface
Paper.
5. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 4, wherein, institute
The preparation method for stating chitosan/titanium dioxide composite solution is as follows：
Chitosan is dissolved in 1.00% glacial acetic acid solution, ultrasonic wave dissolving is opened, adds n-hydroxysuccinimide, continue
Open ultrasonic wave to mix, add EDC and stir to being completely dissolved, add arginine, continue stirring 20h~24h and obtain reaction solution,
Reaction solution is subjected to dialysis treatment, freeze-drying obtains, through the modified chitosan of arginine, weighing nano titanium oxide powder
It is dissolved in deionized water, ultrasonic 20min obtains titanium dioxide emulsion, adds modified chitosan, continues on ultrasound
Ripple ultrasound, is made chitosan/titanium dioxide composite solution.
6. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 5, wherein, institute
It is to immerse bag filter in distilled water fully to absorb water to state dialysis treatment, and reaction solution is fitted into bag filter and dialysed 2 days with distilled water,
Period changed first water at interval of 8 hours, is finally put into bag filter in 16% polyglycol solution and concentrates 24h.
7. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 6, wherein, institute
The crystalline structure for stating nano titanium oxide is Detitanium-ore-type, and grain size is 20nm~50nm.
8. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 7, wherein, institute
The mass concentration for stating titanium dioxide emulsion is 45%~55%.
9. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 8, wherein, institute
The mass ratio for stating titanium dioxide and modified chitosan is 3:1.
10. a kind of preparation method of urine detection test paper for reacting fatigue level of human body according to claim 9, wherein,
In the chitosan/titanium dioxide composite solution preparation method add modification of chitosan after ultrasound parameter for frequency 25kHz~
35kHz, power 110W~150W, ultrasonic time are 20min~30min.
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| CN201710533850.1A Pending CN107340290A (en) | 2017-07-03 | 2017-07-03 | A kind of urine detection test paper for reacting fatigue level of human body and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108445209A (en) * | 2018-03-19 | 2018-08-24 | 南京博天科智生物技术有限公司 | A kind of cTnI Test paper |
| CN108445205A (en) * | 2018-03-19 | 2018-08-24 | 南京博天科智生物技术有限公司 | A kind of preparation method of cTnI Test paper |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445209A (en) * | 2018-03-19 | 2018-08-24 | 南京博天科智生物技术有限公司 | A kind of cTnI Test paper |
| CN108445205A (en) * | 2018-03-19 | 2018-08-24 | 南京博天科智生物技术有限公司 | A kind of preparation method of cTnI Test paper |
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Application publication date: 20171110 |