CN107338239A - A kind of method that furfural dregs immobilization carries the preparation method and applications and immobilised enzymes of enzyme material - Google Patents
A kind of method that furfural dregs immobilization carries the preparation method and applications and immobilised enzymes of enzyme material Download PDFInfo
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Abstract
The invention discloses the preparation method that a kind of furfural dregs immobilization carries enzyme material, its step is (1) pretreatment of raw material；(2) preparation of the Chitosan Composites of furfural dregs one；(3) cross-linking reaction of furfural dregs chitosan glutaraldehyde.The method that the application and immobilised enzymes of enzyme material are carried the invention also discloses furfural dregs immobilization.The present invention is combined by furfural dregs and chitosan, furfural dregs and chitosan are carried out water-soluble scattered, compound immobilised enzymes material better than single carbon-based adsorption material is prepared by electrostatic self-assembled technology, present invention incorporates the advantage of furfural dregs and chitosan, functional group with more horn of plenty, it is simultaneously more excellent with the electrostatic self-assembled effect of chitosan, this method is raw materials used cheap and easy to get, preparation method is simple, properties of product are stable, repeat and recycle after the furfural dregs composite immobilised enzymes, recovery is convenient, it is environmentally safe, waste residue is carried out to reuse, meet the requirement of Green Chemistry.
Description
Technical field
The invention belongs to environmental project waste residue utilization technical field, and in particular to one kind prepares immobilization using furfural dregs and carried
The method of enzyme material.The invention further relates to the application that the furfural dregs immobilization carries enzyme material immobilized enzyme.
Background technology
Furfural dregs are that poly-pentose composition pyrohydrolysis produces furans first in the agricultural byproducts processing residue such as corncob, rice husk
The solid slag obtained after aldehyde, containing substantial amounts of cellulose, hemicellulose and lignin, there is good recycle value.According to
Report, the furfural wet slag generation that 1t furfurals just have more than 10 tons is often produced, China's furfural dregs annual emissions are up to 3,000,000 t.By
It is high in furfural dregs salt content, in acidity, long-term outdoor accumulation, occupation of land problem can be not only produced, can also give air, soil, river
Stream etc. brings pollution, therefore the recycling problem urgent need to resolve of furfural dregs.Furfural dregs pH value is about 2, and natural air drying is in Huang
Brown, quality is soft, and its main component is cellulose, hemicellulose, lignin and a small amount of nitrogen, P elements etc., is contained
There are the functional groups such as substantial amounts of hydroxyl, carboxyl, there is good chemical reactivity.Therefore, pectase is fixed not using furfural dregs
Recycling can be only realized, practical application that again can be for furfural dregs in terms of solid enzyme provides certain theoretical foundation and reference
Foundation.
The content of the invention
For above-mentioned prior art, the technical problem to be solved in the present invention is to realize recycling using furfural dregs,
Use the method for furfural dregs immobilised enzymes.
The present invention solves above-mentioned technical problem by the following technical programs,
A kind of furfural dregs immobilization carries the preparation method of enzyme material, and its step is,
(1) pretreatment of raw material, the impurity and the hydrotrope on furfural dregs surface are rinsed out repeatedly with water, afterwards 80 DEG C of drying 24h,
Crushed again on coal pulverizer, sieving, obtain 40-100 mesh particles；
(2) preparation of furfural dregs-Chitosan Composites, by the chitosan solution that 15-40ml mass fraction is 2-5%
Add in 50mL small beaker, ultrasonic 20-40min and magnetic agitation 12h, be centrifuged repeatedly afterwards with distilled water with 1.0g furfural dregs
Washing is multiple, 80 DEG C of drying 12h, then is slightly ground into 40-100 mesh particles, and furfural dregs-Chitosan Composites are made；
(3) cross-linking reaction of furfural dregs-chitosan-glutaraldehyde, by the glutaraldehyde and 1.0g furfurals that mass fraction is 4-5%
Slag-Chitosan Composites in mass ratio 0.1-0.5: 1 are added in small beaker, ultrasonic 20-40min and magnetic agitation crosslinking 10-
12h, it is centrifuged repeatedly washing repeatedly with distilled water afterwards, 80-100 DEG C of drying 10-20h, that is, furfural dregs immobilization is made and carries enzyme material
Material.
As optimization, also containing the acetic acid that mass fraction is 1-3% in chitosan solution described in step (2).
The invention solves another technical problem be furfural dregs immobilization carry enzyme material application and immobilised enzymes
Method.
Furfural dregs immobilization carries the application of enzyme material, it is characterised in that the carrier application as immobilised enzymes.
As optimization, the immobilised enzymes is immobilization pectase.
The method that furfural dregs immobilization carries enzyme material immobilized enzyme, its step is,
(1) weigh the immobilization of 0.05g furfural dregs and carry enzyme material, be placed in 10mL centrifuge tubes, add 10mg/mL pectin
Enzyme 2-6mL；
(2) centrifugation is taken out after 4h is shaken on the constant temperature oscillator that water temperature is 37 DEG C, supernatant retains；
(3) take the filter residue after centrifugation first to be washed twice with the centrifugation of distillation water washing once, then with pH=3.5 cushioning liquid,
Furfural dregs immobilization is made and carries the pectase that enzyme material is fixed.
As optimization, after step (3), by the supernatant obtained in step (2) and furfural dregs immobilization described in step (3)
Carry the pectase that enzyme material is fixed and be positioned over 4 DEG C of refrigerators preservations, to prepare the pectase that furfural dregs immobilization carries enzyme material and fixed
Same day enzyme activity is 100% meter, and the relative enzyme activity that furfural dregs immobilization carries the pectase that enzyme material is fixed when preserving 6 days is 88.76-
90.08%；
As optimization, after step (3), by the supernatant obtained in step (2) and furfural dregs immobilization described in step (3)
Carry the pectase that enzyme material is fixed and be positioned over 4 DEG C of refrigerators preservations, to prepare the pectase that furfural dregs immobilization carries enzyme material and fixed
Same day enzyme activity is 100% meter, and the relative enzyme activity that furfural dregs immobilization carries the pectase that enzyme material is fixed when preserving 15 days is
79.88-80.28%；
As optimization, after step (3), by the supernatant obtained in step (2) and furfural dregs immobilization described in step (3)
Carry the pectase that enzyme material is fixed and be positioned over 4 DEG C of refrigerators preservations, to prepare the pectase that furfural dregs immobilization carries enzyme material and fixed
Same day enzyme activity is 100% meter, and the relative enzyme activity that furfural dregs immobilization carries the pectase that enzyme material is fixed when preserving 21 days is
74.32-75.52%；
As optimization, after step (3), by the supernatant obtained in step (2) and furfural dregs immobilization described in step (3)
Carry the pectase that enzyme material is fixed and be positioned over 4 DEG C of refrigerators preservations, to prepare the pectase that furfural dregs immobilization carries enzyme material and fixed
Same day enzyme activity is 100% meter, and the relative enzyme activity that furfural dregs immobilization carries the pectase that enzyme material is fixed when preserving 32 days is
70.92-73.12%.
The present invention is combined by furfural dregs and chitosan, i.e., furfural dregs and chitosan progress are water-soluble scattered, by quiet
Electric self-assembling technique prepares the compound immobilised enzymes material better than single carbon-based adsorption material, and present invention incorporates furfural dregs
With the advantage of chitosan, the functional group with more horn of plenty, while, the party more excellent with the electrostatic self-assembled effect of chitosan
Method it is raw materials used it is cheap and easy to get, preparation method is simple, properties of product are stable.Present invention also offers above-mentioned furfural dregs and chitosan
Application in immobilised enzymes, repeat after the furfural dregs composite immobilised enzymes and recycle, recovery is convenient, to environment without
Pollution, carry out waste residue and reuse, meet the requirement of Green Chemistry.
Brief description of the drawings
Fig. 1 is the flow chart that the immobilization of furfural dregs of the embodiment of the present invention carries enzyme material immobilized enzyme；
Fig. 2 is the infrared spectrogram that furfural dregs immobilization prepared by the embodiment of the present invention 1 carries enzyme material；
Fig. 3 is the scanning electron microscope (SEM) photograph that furfural dregs immobilization prepared by the embodiment of the present invention 1 carries enzyme material；
Fig. 4 is the photoelectron spectroscopy figure that furfural dregs immobilization prepared by the embodiment of the present invention 1 carries enzyme material.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in detail.
Embodiment 1
A kind of furfural dregs immobilization carries the preparation method of enzyme material, and its step is,
(1) pretreatment of raw material, the impurity and the hydrotrope on furfural dregs surface are rinsed out repeatedly with water, afterwards 80 DEG C of drying 24h,
Crushed again on coal pulverizer, sieving, obtain 60-80 mesh particles；
(2) preparation of furfural dregs-Chitosan Composites, by the chitosan solution and 1.0g that 25ml mass fractions are 2%
Furfural dregs are added in 50mL small beaker, and washing is centrifuged repeatedly repeatedly with distilled water after ultrasonic 20min, magnetic agitation 12h, 80 DEG C
12h is dried, is slightly ground into 60-80 mesh particles, furfural dregs-Chitosan Composites are made；Also contain in the chitosan solution
There is the acetic acid that mass fraction is 1%；
(3) cross-linking reaction of furfural dregs-chitosan-glutaraldehyde, 4% glutaraldehyde and 1.0g furfural dregs-chitosan is compound
Material in mass ratio 0.1: 1 is added in small beaker, ultrasonic 20min, and washing is centrifuged repeatedly with distilled water after magnetic agitation crosslinking 10h
Repeatedly, 80 DEG C of drying 12h, that is, furfural dregs immobilization is made and carries enzyme material.
Embodiment 2
A kind of furfural dregs immobilization carries the preparation method of enzyme material, and its step is,
(1) pretreatment of raw material, the impurity and the hydrotrope on furfural dregs surface are rinsed out repeatedly with water, afterwards 80 DEG C of drying 24h,
Crushed again on coal pulverizer, sieving, it is standby to obtain 80-100 mesh particles.
(2) preparation of furfural dregs-Chitosan Composites, by the chitosan solution and 1.0g that 40ml mass fractions are 3%
Furfural dregs are added in 50mL small beaker, and washing is centrifuged repeatedly repeatedly with distilled water after ultrasonic 40min, magnetic agitation 12h, 80 DEG C
12h is dried, is slightly ground into 80-100 mesh particles, furfural dregs-Chitosan Composites are made；Also contain in the chitosan solution
There is the acetic acid that mass fraction is 1%；
(3) cross-linking reaction of furfural dregs-chitosan-glutaraldehyde, 5% glutaraldehyde and 1.0g furfural dregs-chitosan is compound
Material in mass ratio 0.3: 1 is added in small beaker, ultrasonic 40min, and washing is centrifuged repeatedly with distilled water after magnetic agitation crosslinking 10h
Repeatedly, 80 DEG C of drying 12h, that is, furfural dregs immobilization is made and carries enzyme material.
Embodiment 3
A kind of furfural dregs immobilization carries the preparation method of enzyme material, and its step is,
(1) pretreatment of raw material, the impurity and the hydrotrope on furfural dregs surface are rinsed out repeatedly with water, afterwards 80 DEG C of drying 24h,
Crushed again on coal pulverizer, sieving, it is standby to obtain 40-60 mesh particles.
(2) preparation of furfural dregs-Chitosan Composites, by the chitosan solution and 1.0g that 15ml mass fractions are 5%
Furfural dregs are added in 50mL small beaker, and washing is centrifuged repeatedly repeatedly with distilled water after ultrasonic 30min, magnetic agitation 12h, 80 DEG C
12h is dried, is slightly ground into 40-60 mesh particles, furfural dregs-Chitosan Composites are made；Also contain in the chitosan solution
There is the acetic acid that mass fraction is 1%；
(3) cross-linking reaction of furfural dregs-chitosan-glutaraldehyde, 5% glutaraldehyde and 1.0g furfural dregs-chitosan is compound
Material in mass ratio 0.5: 1 is added in small beaker, ultrasonic 30min, and washing is centrifuged repeatedly with distilled water after magnetic agitation crosslinking 10h
Repeatedly, 80 DEG C of drying 12h, that is, furfural dregs immobilization is made and carries enzyme material.
Embodiment 4 investigates the relative enzyme activity of immobilization pectase under different Storage periods
The furfural dregs immobilization for weighing embodiment 1-3 preparations carries each 1.3g of enzyme material, and fixing pectase is tested respectively.
The method that furfural dregs immobilization carries the material immobilized pectase of enzyme, its step is,
(1) the furfural dregs immobilization for weighing 0.05g embodiments 1-3 preparations respectively carries enzyme material, is placed in three 10mL centrifugations
Guan Zhong, it is separately added into 10mg/ml pectinase solution 2ml, 4ml, 6ml again in three centrifuge tubes；
(2) centrifugation is taken out after 4h is shaken on the constant temperature oscillator that water temperature is 37 DEG C, supernatant retains；
(3) take the filter residue after centrifugation first to be washed twice with the centrifugation of distillation water washing once, then with pH=3.5 cushioning liquid,
Furfural dregs immobilization is made and carries the pectase that enzyme material is fixed；
(4) supernatant obtained in step (2) is carried to the fruit of enzyme material fixation with furfural dregs immobilization described in step (3)
Glue enzyme is positioned over 4 DEG C of refrigerators and preserved.
Furfural dregs immobilization prepared by embodiment 1 is carried into the material immobilized pectase of enzyme to store under 4 DEG C of environment, to prepare
It is 100% meter that furfural dregs immobilization, which carries the pectase same day enzyme activity that enzyme material is fixed, respectively in storage 6 days, 15 days, 21 days and 32
It when determine relative enzyme activity, relative enzyme activity is 89.14% during storage 6 days；With respect to enzyme activity it is 80.28% during storage 15 days；Storage
With respect to enzyme activity it is 74.32% at 21 days, with respect to enzyme activity is 72.02% during storage 32 days.
Furfural dregs immobilization prepared by embodiment 2 is carried into the material immobilized pectase of enzyme to store under 4 DEG C of environment, to prepare
It is 100% meter that furfural dregs immobilization, which carries the pectase same day enzyme activity that enzyme material is fixed, respectively in storage 6 days, 15 days, 21 days and 32
It when determine relative enzyme activity, relative enzyme activity is 90.08% during storage 6 days；With respect to enzyme activity it is 81.08% during storage 15 days；Storage
With respect to enzyme activity it is 75.52% at 21 days, with respect to enzyme activity is 70.92% during storage 32 days.
Furfural dregs immobilization prepared by embodiment 3 is carried into the material immobilized pectase of enzyme to store under 4 DEG C of environment, to prepare
It is 100% meter that furfural dregs immobilization, which carries the pectase same day enzyme activity that enzyme material is fixed, respectively in storage 6 days, 15 days, 21 days and 32
It when determine relative enzyme activity, relative enzyme activity is 88.76% during storage 6 days；With respect to enzyme activity it is 79.88% during storage 15 days；Storage
With respect to enzyme activity it is 74.60% at 21 days, with respect to enzyme activity is 73.12% during storage 32 days.
Embodiment 5 investigates the load enzyme amount that furfural dregs immobilization carries enzyme material
Three kinds of supernatants of gained are determined with ultraviolet specrophotometer respectively after three centrifuge tubes in embodiment 4 are centrifuged
Its absorbance change value K at 540nm540, substitute into calibration curve equation formula in calculate it is immobilized under respective conditions after supernatant
The quality of the unlocked enzyme remained in liquid, i.e.,：Carrier carries the quality of enzyme amount=(total enzyme amount-loose enzyme amount)/carrier, then
Calculate and carry enzyme amount.
After tested and calculate, furfural dregs immobilization prepared by embodiment 1 carries the material immobilized pectase load enzyme amount of enzyme and is
133.68mg/g；It is 158.78mmg/g that furfural dregs immobilization prepared by embodiment 2, which carries the material immobilized pectase of enzyme and carries enzyme amount,；
It is 197.20mg/g that furfural dregs immobilization prepared by embodiment 3, which carries the material immobilized pectase of enzyme and carries enzyme amount,.
The method that embodiment 6 determines relative enzyme activity
3,5- dinitrosalicylic Acid Colorimetries (abbreviation DNS methods) principle：3,5- dinitrosalicylic acids energy in alkaline environment
It is enough that redox reaction occurs with reducing sugar, amino-compound is reduced into, after being total to hot 5min in boiling water bath, solution can be
There is maximum absorption band under 540nm wavelength.
In the present embodiment experiment, pectin is broken down into reducing sugar D- galacturonic acids after enzyme digestion reaction, with 3,5- bis-
The amino-compound of brownish red is formed after the common heat of nitro-salicylic acid, i.e., there occurs chromogenic reaction.
It is accurate to weigh D- (+)-galacturonic acid 1.00g, the dissolving of pH=3.5 cushioning liquid is added, is transferred to 1000mL appearances
Measuring bottle constant volume, 1mg/ml Standard for Sugars liquid is made as standard sample, makes standard curve.
The relative enzyme activity method of measure：Two 25mL colorimetric cylinders are taken, numbering is 1# and 2# respectively；Added into 1# colorimetric cylinders real
Apply any furfural dregs composite immobilization pectase 0.05g of the preparation of example 4；Add 2mL into 1# and 2# colorimetric cylinders respectively again
PH=3.5 cushioning liquid；Then added into 1# colorimetric cylinders in 10mg/mL pectin 1mL, 2# colorimetric cylinder and be added without pectin,
Two colorimetric cylinders shake up rapidly, after reacting 120min in 45 DEG C of water-baths, then are separately added into two colorimetric cylinders of 1# and 2#
2mol/L NaOH solution 5mL and DNS reagent 1.5mL, then continue to boiling water and boil 5min, then with originally water cooling, and with steaming
Distilled water constant volume；2mL reaction solutions are taken to be contained in the volume labeled as 3# as in 10mL colorimetric cylinders from 1# colorimetric cylinders, from 2# colorimetric cylinders
Also take 2mL reaction solutions to be contained in the volume labeled as 4# as in 10mL colorimetric cylinders, and 3# and 4# colorimetric cylinders are diluted to graduation mark, will
Solution is background reference in 4# colorimetric cylinders, surveys the absorbance of two colorimetric cylinders.3# colorimetric cylinders survey absorbance ratio and pass through standard
Curve calculates enzyme activity.
Embodiment 7
After furfural dregs immobilization load enzyme material prepared by Example 3 presses the method immobilization pectase of embodiment 4, by implementation
The methods described of example 6 carries out pectin catalysis degeneration experiment, and catalytic condition is：The furfural dregs immobilization for taking 50mg embodiments 3 to prepare carries
The material immobilized pectase of enzyme is under the conditions of pH=3.5, temperature is 45 DEG C to initial concentration C0=15mg/mL pectin is urged
Change degraded 120min, refilter and take out the furfural dregs immobilization load material immobilized pectase of enzyme, after determining filtrate absorbance,
Relative enzyme activity when immobilization pectase first time catalytic degradation is calculated is 95.52%.
The furfural dregs immobilization is re-used as stated above carries the material immobilized pectin enzymatic degradation of enzyme 6 times,
The relative enzyme activity for measuring the furfural dregs immobilization load material immobilized pectase of enzyme after reusing 1 time drops to 92.66%,
The relative enzyme activity of the furfural dregs immobilization load material immobilized pectase of enzyme drops to 87.16% after reusing 2 times, repeats
The relative enzyme activity that the material immobilized pectase of enzyme is carried using the furfural dregs immobilization after 3 times drops to 86.37%, reuses
The relative enzyme activity of the furfural dregs immobilization load material immobilized pectase of enzyme drops to 85.45% after 4 times, reuses 5 times
The relative enzyme activity of the furfural dregs immobilization load material immobilized pectase of enzyme drops to 83.62% afterwards, phase after reusing 6 times
81.78% is dropped to enzyme activity.
Embodiment 8 investigates the loading condition that furfural dregs immobilization carries enzyme material
The furfural dregs immobilization prepared in embodiment 1 load enzyme material is done into infrared spectrogram, ESEM, photoelectron spectroscopy
Figure, is shown in Fig. 2-4.
As shown in Figure 2, the infrared spectrogram that enzyme material is carried by furfural dregs and furfural dregs immobilization can obtain to draw a conclusion：①
In 1683cm-1There is new absworption peak in place, and peak intensity is very big, is that the amino of chitosan and the aldehyde radical of glutaraldehyde occur
Another C=0 that the absworption peak and glutaraldehyde for the Schiff key (C=N) that Schiff reacts and formed are not engaged in reaction stretches
Caused by contracting vibration；②1020cm-1The absworption peak at place is also remarkably reinforced, and is the flexural vibrations by N-H and N-C；3. chitosan
In 0-H and N-H stretching vibration cause 3333cm-1The absworption peak at place is remarkably reinforced, 2900cm-1The C-H at place stretching vibration
Absworption peak also has a certain degree of enhancing.
From the figure 3, it may be seen that pretreated furfural dregs are layer structure, its surface is more smooth, and specific surface area is larger.
As shown in Figure 4, understand that furfural dregs immobilization carries enzyme material tests and goes out new spy according to photoelectron spectroscopy elementary analysis
Absworption peak N1s (400eV) is levied, illustrates that chitosan has wrapped up furfural dregs surface and met prepared furfural dregs immobilization load enzyme material
Surface composition feature.
Comparative example investigates the existing load material immobilized pectase situation of enzyme
Taking the too western coal of Ningxia Rujigou ore deposit, it is 56.5mg/g that its maximum, which carries enzyme amount, and is used continuously 7 times as sorbing material
It is only afterwards 32.3% with respect to enzyme activity.
Taking magnetic oxygenated graphene, relative enzyme activity is 58% after being used continuously 7 times as carrier immobilized pectase.
Epoxy microballoon is taken, to being fixed of pectase, the higher immobilization pectase of vigor to be obtained, even as carrier
It is 56.4% to continue relative enzyme activity after using 7 times.
Have preferably operation steady by the furfural dregs immobilization pectase that with being found after comparative example, prepared by the present invention
Qualitative, furfural dregs immobilization prepared by the present invention carries relative enzyme activity of the material immobilized pectase of enzyme after 32d is stored and is maintained at
More than 70%, and there is higher operational stability, after continuous use 7 times, more than 80% is also maintained at respect to enzyme activity.
Above-mentioned combination accompanying drawing is explained in detail to embodiments of the present invention, but the present invention is not limited to above-mentioned implementation
Mode, in one skilled in the relevant art's possessed knowledge, many variations can also be made to it.
Claims (9)
1. a kind of preparation method of furfural dregs immobilization load enzyme material, its step are,
(1) pretreatment of raw material, the impurity and the hydrotrope on furfural dregs surface are rinsed out repeatedly with water, afterwards 80 DEG C of drying 24h, then
Crushed on coal pulverizer, sieving, obtain 40-100 mesh particles；
(2) preparation of furfural dregs-Chitosan Composites, by 15-40ml mass fraction be 2-5% chitosan solution and
1.0g furfural dregs are added in 50mL small beaker, ultrasonic 20-40min and magnetic agitation 12h, are centrifuged repeatedly wash with distilled water afterwards
Wash repeatedly, 80 DEG C of drying 12h, then be slightly ground into 40-100 mesh particles, furfural dregs-Chitosan Composites are made；
(3) cross-linking reaction of furfural dregs-chitosan-glutaraldehyde, by mass fraction be 4-5% glutaraldehyde and 1.0g furfural dregs-
Chitosan Composites in mass ratio 0.1-0.5: 1 are added in small beaker, ultrasonic 20-40min and magnetic agitation crosslinking 10-12h,
Washing is centrifuged repeatedly with distilled water repeatedly, 80-100 DEG C of drying 10-20h, that is, furfural dregs immobilization is made and carries enzyme material afterwards.
2. furfural dregs immobilization as claimed in claim 1 carries the preparation method of enzyme material, it is characterised in that described in step (2)
Also containing the acetic acid that mass fraction is 1-3% in chitosan solution.
3. the furfural dregs immobilization of any preparations of claim 1-2 carries the application of enzyme material, it is characterised in that as fixation
Change the carrier application of enzyme.
4. furfural dregs immobilization as claimed in claim 3 carries the application of enzyme material, it is characterised in that the immobilised enzymes is fixation
Change pectase.
5. the method that the furfural dregs immobilization of any preparations of claim 1-2 carries enzyme material immobilized enzyme, its step are,
(1) weigh the immobilization of 0.05g furfural dregs and carry enzyme material, be placed in 10mL centrifuge tubes, add 10mg/mL pectase 2-
6mL；
(2) centrifugation is taken out after 4h is shaken on the constant temperature oscillator that water temperature is 37 DEG C, supernatant retains；
(3) take the filter residue after centrifugation first to be washed twice with the centrifugation of distillation water washing once, then with pH=3.5 cushioning liquid, that is, make
Obtain furfural dregs immobilization and carry the pectase that enzyme material is fixed.
6. the method that furfural dregs immobilization as claimed in claim 5 carries enzyme material immobilized enzyme, it is characterised in that after step (3),
The pectase that the supernatant obtained in step (2) is carried to the fixation of enzyme material with furfural dregs immobilization described in step (3) is positioned over 4
DEG C refrigerator preserves, and carry the pectase same day enzyme activity that enzyme material fixes to prepare furfural dregs immobilization counts as 100%, during preservation 6 days
The relative enzyme activity that furfural dregs immobilization carries the pectase that enzyme material is fixed is 88.76-90.08%.
7. the method that the furfural dregs immobilization as described in claim 5 or 6 carries enzyme material immobilized enzyme, it is characterised in that step (3)
Afterwards, the pectase for the supernatant obtained in step (2) being carried to the fixation of enzyme material with furfural dregs immobilization described in step (3) is placed
Preserved in 4 DEG C of refrigerators, the pectase same day enzyme activity fixed using preparing furfural dregs immobilization load enzyme material is counted as 100%, preserves 15
It when furfural dregs immobilizations to carry the relative enzyme activity of the pectase that enzyme material is fixed be 79.88-80.28%.
8. the method that the furfural dregs immobilization as described in claim 5 or 6 carries enzyme material immobilized enzyme, it is characterised in that step (3)
Afterwards, the pectase for the supernatant obtained in step (2) being carried to the fixation of enzyme material with furfural dregs immobilization described in step (3) is placed
Preserved in 4 DEG C of refrigerators, the pectase same day enzyme activity fixed using preparing furfural dregs immobilization load enzyme material is counted as 100%, preserves 21
It when furfural dregs immobilizations to carry the relative enzyme activity of the pectase that enzyme material is fixed be 74.32-75.52%.
9. the method that the furfural dregs immobilization as described in claim 5 or 6 carries enzyme material immobilized enzyme, it is characterised in that step (3)
Afterwards, the pectase for the supernatant obtained in step (2) being carried to the fixation of enzyme material with furfural dregs immobilization described in step (3) is placed
Preserved in 4 DEG C of refrigerators, the pectase same day enzyme activity fixed using preparing furfural dregs immobilization load enzyme material is counted as 100%, preserves 32
It when furfural dregs immobilizations to carry the relative enzyme activity of the pectase that enzyme material is fixed be 70.92-73.12%.
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