CN107334818A - A kind of pharmaceutical composition and preparation method and purposes for being used to improve immune function of human body - Google Patents
A kind of pharmaceutical composition and preparation method and purposes for being used to improve immune function of human body Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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Abstract
The invention discloses a kind of pharmaceutical composition and preparation method thereof for being used to improve immune function of human body, described pharmaceutical composition by following components and weight than raw material form：The parts by weight of radix fici simplicissimae 8~80, the parts by weight of Radix Angelicae Sinensis 5~60, the parts by weight of ganoderma lucidum 1~30.It is shown experimentally that, the pharmaceutical composition has the function that to improve immune function of human body, influence to indexs such as the phagocyte imdex of immunosuppressed mice, phagocytic index, Thymus and Spleen indexes is better than compatibility group two-by-two, the results showed that has the function that Synergistic between each component.In addition, TC, TG that pharmaceutical composition of the present invention then can be reduced significantly in mice serum are horizontal, there is reducing blood lipid.
Description
Technical field
The invention belongs to drug field, is related to a kind of pharmaceutical composition for being used to improve immune function of human body, particularly relates to
It is and a kind of using traditional Chinese medicine decoction piece extract as the pharmaceutical composition and preparation method for being used to improve body immunity made of raw material.
Background technology
Immunity is the defense mechanism of human body itself, is that human bioequivalence and any foreign matter of the external intrusion of elimination are (viral, thin
Bacterium etc.), processing aging, damage, death, the own cells of denaturation and identification and processing vivo mutations cell and virus are infected thin
The ability of born of the same parents.When immune function of human body is low, just extremely easily cause the infection such as virus, bacterium, fungi, human body is easy for out
A series of diseases such as existing fatigue, flu.Radix fici simplicissimae is commonly used for the replacement of the Radix Astragali for the dry root of Moraceae ficus species ficus simplicissima
Product, therefore also known as southern stilbene, it is a kind of plant of medicine-food two-purpose, has and replenish qi to invigorate the spleen, eliminating the phlegm dampness elimination, the effect of relaxing tendons and activating collaterals, is used for
Deficiency syndrome of the lung asthma due to excessive phlegm, deficiency of spleen-QI and stomach-QI, limb is tired powerless, deficiency of food abdominal distension, oedema, under band, arthralgia pain due to rheumatism, and pain in waist and lower extremities.Planted when being classified as Umbelliferae
The root of thing Radix Angelicae Sinensis, has a benefiting blood and regulating blood circulation, menstruction regulating and pain relieving, the effect of moisturize laxation.Ganoderma lucidum is bracket fungus fungi red sesame or purple sesame
Fructification is dried, there is the effect of invigorating qi for tranquilization, relieving cough and asthma.Now exempt from there are some researches show ganoderma lucidum tool is improved in varying degrees human body
The effect of epidemic disease power, but have no the report on radix fici simplicissimae, Radix Angelicae Sinensis and ganoderma lucidum compatibility.
The content of the invention
It is an object of the invention to solve the deficiencies in the prior art, there is provided and one kind composition is simple, and by natural medicinal raw material
Combination of active principles form have improve immune function of human body pharmaceutical composition.The pharmaceutical composition has good effect,
Have no toxic side effect, be not likely to produce tolerance, it is convenient to take, it is generally applicable to the patient of immunologic hypofunction.
It is another object of the present invention to provide a kind of preparation method of pharmaceutical composition and purposes.The mesh of the present invention
Be achieved through the following technical solutions：A kind of pharmaceutical composition for being used to improve immune function of human body, it is characterised in that：
It by following components and weight than raw material form：
A kind of pharmaceutical composition for being used to improve immune function of human body, it is characterised in that：It is by following components and weight
The raw material composition of ratio：The parts by weight of radix fici simplicissimae 8~80, the parts by weight of Radix Angelicae Sinensis 5~60, the parts by weight of ganoderma lucidum 1~30；Preferably, the five fingers
The parts by weight of wild peach 10~60, the parts by weight of Radix Angelicae Sinensis 8~50, the parts by weight of ganoderma lucidum 3~25；Preferably, the parts by weight of radix fici simplicissimae 15~40,
The parts by weight of Radix Angelicae Sinensis 10~40, the parts by weight of ganoderma lucidum 5~20；Preferably, the parts by weight of radix fici simplicissimae 20~35, the weight of Radix Angelicae Sinensis 15~30
Part, the parts by weight of ganoderma lucidum 8~15；Preferably, the parts by weight of radix fici simplicissimae 30, the parts by weight of Radix Angelicae Sinensis 20, the parts by weight of ganoderma lucidum 10.
A kind of pharmaceutical composition for being used to improve immune function of human body, it is characterised in that：It is by following components and weight
The raw material composition of ratio：The parts by weight of radix fici simplicissimatis extractive 8~80, the parts by weight of angelica extract 5~60, Ganodenna Lucidum P.E 1~30
Parts by weight.Preferably, the parts by weight of radix fici simplicissimatis extractive 30, the parts by weight of angelica extract 20, the parts by weight of Ganodenna Lucidum P.E 10.
A kind of preparation method for being used to improve the pharmaceutical composition of immune function of human body, it is characterised in that：It includes following
Step：
S1：Weigh raw material：Weighed by said components and weight ratio and refer to wild peach, Radix Angelicae Sinensis and ganoderma lucidum；
S2：After raw material is well mixed, adds pharmaceutically acceptable auxiliary material and be prepared into pharmaceutically conventional pharmaceutical preparation.
The pharmaceutical composition of the present invention can be prepared into institute in any pharmacy according to the conventional method of pharmaceutical field
The formulation stated；The pharmaceutical composition of the present invention can be applied to patient by way of oral, suction or parenteral administration.Orally give
Piece agent, capsule, pill, pulvis, granule, syrup or oral liquid etc. can be prepared during medicine；It can be made in parenteral administration
It is standby into freeze-dried powder and parenteral solution etc..
Pharmaceutically acceptable auxiliary material of the present invention includes starch, magnesium stearate, dextrin and microcrystalline cellulose.
Present invention also offers the pharmaceutical composition to prepare the purposes in being used to improve immune function of human body medicine.
To enable above-mentioned formulation to realize, pharmaceutically acceptable auxiliary material need to be added when preparing these formulations, such as：Filling
Agent, disintegrant, lubricant, suspending agent, adhesive, sweetener, flavouring, preservative etc., filler includes：Starch, pregelatinated
Starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose etc., disintegrant includes：Starch, pregelatinized starch, microcrystalline cellulose
Element, sodium carboxymethyl starch, PVPP, low-substituted hydroxypropyl cellulose, Ac-Di-Sol etc., lubrication
Agent includes：Magnesium stearate, lauryl sodium sulfate, talcum powder, silica etc., suspending agent includes：It is polyvinylpyrrolidone, micro-
Crystalline cellulose, sucrose, agar, hydroxypropyl methyl cellulose etc., adhesive includes, starch slurry, polyvinylpyrrolidone, hydroxypropyl
Methylcellulose etc., sweetener includes：Saccharin sodium, aspartame, sucrose, honey element, enoxolone etc., flavouring includes：Sweet tea
Taste agent and various essence, preservative include：Parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, vinegar
Sour chloroethene is fixed, eucalyptus oil etc..
The beneficial effects of the invention are as follows：Pharmaceutical composition has the function that to improve immune function of human body, small to immunosupress
The influence of the indexs such as the phagocyte imdex of mouse, phagocytic index, Thymus and Spleen index is better than compatibility group two-by-two, as a result
Show that there is Synergistic between each component.In addition, pharmaceutical composition of the present invention then can significantly reduce mice serum
In TC, TG it is horizontal, there is reducing blood lipid.
Embodiment
Technical scheme is described in further detail with reference to embodiment, but protection scope of the present invention is not limited to
In as described below.
Embodiment 1：
Raw material radix fici simplicissimae 8g, Radix Angelicae Sinensis 5g, ganoderma lucidum 1g are weighed, pharmaceutic adjuvant is added according to a conventional method and particle is uniformly made,
Tabletting, obtain tablet.
Embodiment 2：
Raw material radix fici simplicissimae 80g, Radix Angelicae Sinensis 60g, ganoderma lucidum 30g are weighed, adds pharmaceutic adjuvant uniformly obtained according to a conventional method
Grain, tabletting, obtains tablet.
Embodiment 3：
Raw material radix fici simplicissimae 10g, Radix Angelicae Sinensis 8g, ganoderma lucidum 3g are weighed, pharmaceutic adjuvant is added according to a conventional method and particle is uniformly made,
Load capsule, obtain capsule.
Embodiment 4：
Raw material radix fici simplicissimae 60g, Radix Angelicae Sinensis 50g, ganoderma lucidum 25g are weighed, adds pharmaceutic adjuvant uniformly obtained according to a conventional method
Grain, load capsule, obtain capsule.
Embodiment 5：
Raw material radix fici simplicissimae 15g, Radix Angelicae Sinensis 10g, ganoderma lucidum 5g are weighed, adds pharmaceutic adjuvant uniformly obtained according to a conventional method
Grain, tabletting, obtains tablet.
Embodiment 6：
Raw material radix fici simplicissimae 40g, Radix Angelicae Sinensis 40g, ganoderma lucidum 20g are weighed, particle is uniformly made according to a conventional method, tabletting, obtains
Tablet.
Embodiment 7：
Raw material radix fici simplicissimae 20g, Radix Angelicae Sinensis 15g, ganoderma lucidum 8g are weighed, particle is uniformly made according to a conventional method, tabletting, obtains piece
Agent.
Embodiment 8：
Raw material radix fici simplicissimae 35g, Radix Angelicae Sinensis 30g, ganoderma lucidum 15g are weighed, particle is uniformly made according to a conventional method, tabletting, obtains
Tablet.
Embodiment 9：
Raw material radix fici simplicissimae 30g, Radix Angelicae Sinensis 20g, ganoderma lucidum 10g are weighed, particle is uniformly made according to a conventional method, tabletting, obtains
Tablet.
Embodiment 10：
Raw material radix fici simplicissimatis extractive 8g, angelica extract 5g, Ganodenna Lucidum P.E 1g are weighed, adds supplementary product starch 15g systems
Grain, magnesium stearate 2g, dextrin 15, microcrystalline cellulose 15g, particle is uniformly made, loads capsule, obtain capsule.
Embodiment 11：
Raw material radix fici simplicissimatis extractive 80g, angelica extract 60g, Ganodenna Lucidum P.E 30g are weighed, adds supplementary product starch
200g pelletizes, magnesium stearate 20g, dextrin 100g, microcrystalline cellulose 100g, and particle is uniformly made, tabletting, obtains tablet.
Embodiment 12：
Raw material radix fici simplicissimatis extractive 30g, angelica extract 20g, Ganodenna Lucidum P.E 10g are weighed, adds supplementary product starch 60g
Granulation, magnesium stearate 3g, dextrin 30g, microcrystalline cellulose 30g, particle is uniformly made, tabletting, obtains tablet.
Embodiment 13：
Raw material radix fici simplicissimatis extractive 70g, angelica extract 50g, Ganodenna Lucidum P.E 35g are weighed, adds supplementary product starch
200g pelletizes, magnesium stearate 20g, dextrin 150g, microcrystalline cellulose 150g, and particle is uniformly made, tabletting, obtains tablet.
Embodiment 14：
Raw material radix fici simplicissimae water extract 8g, Radix Angelicae Sinensis water extract 5g, ganoderma lucidum water extract 1g are weighed, adds supplementary product starch
15g pelletizes, magnesium stearate 2g, dextrin 15, microcrystalline cellulose 15g, and particle is uniformly made, and loads capsule, obtains capsule.
Embodiment 15：
Raw material radix fici simplicissimae water extract 80g, Radix Angelicae Sinensis water extract 60g, ganoderma lucidum water extract 30g are weighed, adds auxiliary material
Starch 200g pelletizes, magnesium stearate 20g, dextrin 100g, microcrystalline cellulose 100g, and particle is uniformly made, tabletting, obtains tablet.
Embodiment 16：
Raw material radix fici simplicissimae ethanol extract 30g is weighed, Radix Angelicae Sinensis ethanol extract 20g, ganoderma lucidum ethanol extract 10g, is added
Enter supplementary product starch 60g granulations, magnesium stearate 3g, dextrin 30g, microcrystalline cellulose 30g, particle is uniformly made, tabletting, obtains piece
Agent.
Embodiment 17：
Raw material radix fici simplicissimae ethanol extract 8g, Radix Angelicae Sinensis ethanol extract 5g, ganoderma lucidum ethanol extract 1g are weighed, routinely
Method adds pharmaceutic adjuvant and particle is uniformly made, and tabletting, obtains tablet.
Embodiment 18：
Raw material radix fici simplicissimae ethanol extract 80g is weighed, Radix Angelicae Sinensis ethanol extract 60g, ganoderma lucidum ethanol extract 30g, is pressed
Conventional method adds pharmaceutic adjuvant and particle is uniformly made, and tabletting, obtains tablet.
Embodiment 19：
Weigh raw material radix fici simplicissimae n-butanol extract 10g, Radix Angelicae Sinensis n-butanol extract 8g, ganoderma lucidum n-butanol extract
3g, pharmaceutic adjuvant is added according to a conventional method particle is uniformly made, loaded capsule, obtain capsule.
Embodiment 20：
Weigh raw material radix fici simplicissimae n-butanol extract 60g, Radix Angelicae Sinensis n-butanol extract 50g, ganoderma lucidum n-butanol extract
25g, pharmaceutic adjuvant is added according to a conventional method particle is uniformly made, loaded capsule, obtain capsule.
Embodiment 21：
Weigh raw material radix fici simplicissimae ligroin extraction 15g, Radix Angelicae Sinensis ligroin extraction 10g, ganoderma lucidum ligroin extraction
5g, pharmaceutic adjuvant is added according to a conventional method particle is uniformly made, tabletting, obtain tablet.
Embodiment 22：
Weigh raw material radix fici simplicissimae ligroin extraction 40g, Radix Angelicae Sinensis ligroin extraction 40g, ganoderma lucidum ligroin extraction
20g, particle is uniformly made according to a conventional method, tabletting, obtains tablet.
Embodiment 23：
Weigh raw material radix fici simplicissimae ligroin extraction 20g, Radix Angelicae Sinensis ligroin extraction 15g, ganoderma lucidum ligroin extraction
8g, particle is uniformly made according to a conventional method, tabletting, obtains tablet.
Embodiment 24：
Weigh raw material radix fici simplicissimae ethyl acetate extract 35g, Radix Angelicae Sinensis ethyl acetate extract 30g, ganoderma lucidum ethyl acetate
Extract 15g, particle is uniformly made according to a conventional method, tabletting, obtains tablet.
Embodiment 25：
Weigh raw material radix fici simplicissimae ethyl acetate extract 30g, Radix Angelicae Sinensis ethyl acetate extract 20g, ganoderma lucidum ethyl acetate
Extract 10g, particle is uniformly made according to a conventional method, tabletting, obtains tablet.
Above-mentioned tablet, capsule can be prepared into specification as 5mg/ pieces, 10mg/ pieces, 20mg/ according to customary preparation methods
Piece, 0.5g/ pieces, or specification is prepared into as different sizes such as 5mg/ grains, 10mg/ grains, 20mg/ grains, 0.5g/ grains.
Beneficial effects of the present invention are proved below by specific pharmacy test：
First, the influence of model mice immunologic function endoxan is injected intraperitoneally
1. research purpose：Ling Gui Johnson ＆ Johnson particle is by radix fici simplicissimae, Radix Angelicae Sinensis and ganoderma lucidum, and dose ratio is radix fici simplicissimae：When
Return：Ganoderma lucidum=3:2:1 (similarly hereinafter), investigate the influence that Ling Gui Johnson ＆ Johnson's particles cause immunosuppressed mice immunologic function to endoxan.
2. experiment material
2.1 experimental animal
160 SPF level KM mouse, full ♂, 20 ± 2g of body weight are real by People's Hospital, Sichuan Prov. of Sichuan Academy of Medical Sciences
Institute of Botany offer, animal credit number are provided：SCXK (river) 2013-15.
2.2 medicines and reagent
Ling Gui Johnson ＆ Johnson particle is made up of radix fici simplicissimae, Radix Angelicae Sinensis and ganoderma lucidum, and drug dose ratio is radix fici simplicissimae：Radix Angelicae Sinensis：Spirit
Sesame=3:2:1, from pharmaceutical college of Chengdu University of Traditional Chinese Medicine preparation research room；Parenteral solution endoxan, the permanent auspicious medical stock in Jiangsu
Part Co., Ltd, numbering：111819；India ink, Shanghai Ming Bo bio tech ltd.
2.3 instrument and equipment
FA1204L electronic balances, Shanghai Yue Ping scientific instrument Co., Ltd；WH-3 vortex oscillation instrument, the analysis of Shanghai Luxi
Instrument Co., Ltd., Factory；TDZ5-WS centrifuges, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.；HH.W21-420S thermostatted waters
Bath cabinet, Shanghai leap Medical Devices Co., Ltd.；The limited public affairs of optical instrument of holding high up are composed in PQF-60 type research fluorescence microscopes, Shanghai
Department.
3. experimental method
3.1 packet administrations
After 220 mouse adaptability nursing 3d 11 groups, i.e. blank group, model group, radix fici simplicissimae will be randomly divided into by body weight
Group (3.2g/kg), ganoderma lucidum group (3.2g/kg), when be grouped into (3.2g/kg), radix fici simplicissimae+ganoderma lucidum group (3.2g/kg), radix fici simplicissimae
+ when be grouped into (3.2g/kg), ganoderma lucidum+when be grouped into (3.2g/kg), Ling Gui Johnson ＆ Johnson particle high (6.4g/kg), in (3.2g/kg), low
(1.6g/kg) three dosage groups, every group 20.Daily gavage gives relative medicine 1 time, and blank group and model group are given in equal volume
Distilled water, administered volume 10ml/kg, successive administration 10d.In addition to blank group, remaining each group is in administration 2d intraperitoneal injections
The endoxan that 75mg/kg is newly prepared, continuous 3d prepare immunosuppressed mice model.
3.2 Carbon grain Kuo clear law determines non-specific immune function
After 11d last doses 30min, 10 mouse tail vein injection india ink 0.01ml/g (injections are randomly selected
It is preceding to use 10 times of normal saline dilution), 15min eye sockets take blood 40ul to add in 4ml 0.1%NaCO3 solution, after fully mixing
Absorbance is determined at 600nm, using 0.1%NaCO3 solution as blank well, calculates phagocyte imdex and phagocytic index.
3.3 Thymus and Spleen indexes detect
By 1h after the remaining 10 mouse administration of each group, take off cervical vertebra and put to death, then clip thymus gland and spleen, and weigh immediately,
Calculate its Thymus and Spleen index.
4. experimental result：
Influence of the 4.1 Ling Gui Johnson ＆ Johnson particles to immunosuppressed mice organ index
Influence of the Ling Gui Johnson ＆ Johnson particle of table 1 to immunosuppressed mice organ index
Note：Compared with blank group,#P<0.05,##P<0.01；Compared with model group,*P<0.05,**P<0.01。
As shown in Table 1, compared with blank group, model group mouse phagocyte imdex, phagocytic index, thymus index and spleen
Index is all decreased obviously (P<0.05) the i.e. model success of model mice immunologic hypofunction, is illustrated；Compared with model group, respectively two-by-two
Compatibility group and each dosage group of Ling Gui Johnson ＆ Johnson particle can significantly improve These parameters (P<0.05), wherein high, middle dose group carbon granules
Clean up index, phagocytic index and index and spleen index and have notable significant difference (P<0.01), ganoderma lucidum group and when be grouped into mouse thymus
Also has significant difference (P with index and spleen index<0.05)；In addition each dosage of Ling Gui Johnson ＆ Johnson particle is to immunosuppressed mice These parameters
Influence be better than compatibility group two-by-two, and the latter is better than three taste prescriptions use, has after prompting Ling Gui Johnson ＆ Johnson particle each component compatibility
There is synergistic function.Data above shows there is Synergistic between the herbal medicine of Ling Gui Johnson ＆ Johnson particle three, leads to simultaneously
After crossing dose compatibility of the present invention, total dosage of Ling Gui Johnson ＆ Johnson particle is administered by 1.6g/kg, and its curative effect is substantially better than the five fingers hair
Peach, ganoderma lucidum and Radix Angelicae Sinensis be used alone or combination of two application after (by 3.2g/kg dosage be administered) the effect of, and with count
Learn meaning.
2nd, to the influence of immunosuppressed mice immunologic function
1. research purpose：Investigate Ling Gui Johnson ＆ Johnson's particles (radix fici simplicissimae, ganoderma lucidum, Radix Angelicae Sinensis) and immunosupress is caused to hydrocortisone
The influence of T lymphocyte subsets in spleen of mice immunized.
2. experiment material
2.1 experimental animal
110 SPF level KM mouse, full ♂, 20 ± 2g of body weight are real by People's Hospital, Sichuan Prov. of Sichuan Academy of Medical Sciences
Institute of Botany offer, animal credit number are provided：SCXK (river) 2013-15.
2.2 medicines and reagent
Ling Gui Johnson ＆ Johnson particle (radix fici simplicissimae, ganoderma lucidum, Radix Angelicae Sinensis) is made by oneself according to a conventional method；Hydrocortisone parenteral solution, Tianjin
Jin Yao pharmaceutcal corporation, Ltds；CD3, CD4, CD8 monoclonal antibody, FITC- rabbit-anti rat monoclonal antibodies, are purchased from Wuhan Biological Products Inst.；
Lymphocytes separating solution, the production of the factory of Shanghai reagent two.
2.3 instrument and equipment
FA1204L electronic balances, Shanghai Yue Ping scientific instrument Co., Ltd；WH-3 vortex oscillation instrument, the analysis of Shanghai Luxi
Instrument Co., Ltd., Factory；TDZ5-WS centrifuges, Hunan Xiang Yi Laboratory Instruments development corporation, Ltd.；HH.W21-420S thermostatted waters
Bath cabinet, Shanghai leap Medical Devices Co., Ltd.；The limited public affairs of optical instrument of holding high up are composed in PQF-60 type research fluorescence microscopes, Shanghai
Department.
3. experimental method
3.1 packet administrations
After 160 mouse adaptability nursing 3d 8 groups, i.e. blank group, model group, radix fici simplicissimae will be randomly divided into by body weight
Group, ganoderma lucidum group, work as grouping, radix fici simplicissimae+ganoderma lucidum group, radix fici simplicissimae+when be grouped into, ganoderma lucidum+when be grouped into, Ling Gui Johnson ＆ Johnson particle is high,
In, low three dosage groups, every group 10.Daily gavage gives relative medicine 1 time, and blank group and model group give isometric distillation
Water, administered volume 10ml/kg, successive administration 10d.In addition to blank group, remaining each group is in administration 2d intraperitoneal injections 75mg/kg
The endoxan newly prepared, continuous 3d prepare immunosuppressed mice model [1].
3.2T lymphocyte subgroups percentage detects
Whole blood lymphocyte is separated with lymphocytes separating solution, it is 1 × 107/ml then to adjust cell concentration.Take
100ul is added in test tube, adds 100ul rat anti-mouse CD3 or CD4 or CD8 monoclonal antibodies, 4 DEG C of incubation 30min, then use
Hanks liquid is washed 3 times, and often pipe adds the rabbit-anti rat monoclonal antibody of 100ul fluorescein isothiocyanates (FITC) mark, then 4 DEG C are incubated
30min, Hanks liquid drip piece after washing 3 times, and 200 lymphocytes are observed under fluorescence microscope, calculate fluorecyte percentage.
4. experimental result
Influence of the 4.1 Ling Gui Johnson ＆ Johnson particles to immunosuppressed mice t lymphocyte subset group
Influence of the Ling Gui Johnson ＆ Johnson particle of table 2 to immunosuppressed mice t lymphocyte subset group
Note：Compared with blank group,#P<0.05,##P<0.01；Compared with model group,*P<0.05,**P<0.01。
As shown in Table 2, compared with blank group, model group mouse CD3, CD4, CD8 percentage substantially reduces, CD4/CD8 ratios
Value is obviously reduced.Compared with model group, ganoderma lucidum group, when be grouped into, two-by-two compatibility group and each dosage group CD3 of Ling Gui Johnson ＆ Johnson particle,
CD4, CD8 percentage substantially rise, and wherein the high middle dose group of Ling Gui Johnson ＆ Johnson particle has a great influence to CD3 and CD4 percentage, and five
Refer to wild peach group CD4 percentage and also have significant difference；In addition each dosage of Ling Gui Johnson ＆ Johnson particle is to immunosuppressed mice These parameters
Influence be better than compatibility group two-by-two, and the latter is better than three taste prescriptions use, prompts Ling Gui Johnson ＆ Johnson that particle each component has collaboration
Synergistic effect.Result above shows that Ling Gui Johnson ＆ Johnson particle can improve t lymphocyte subset group number in immunosuppressed mice body.
3rd, the influence horizontal to hyperlipidemia model lipid of mice caused by high lipoprotein emulsion
1. experimental animal and packet
With experiment one.
2. experimental method
11d, administered volume 0.2mL/10g is administered in each group animal continuous gavage.Give and distill except the daily gavage of blank group
Water 2 times is outer, and remaining each group morning gavage gives high lipoprotein emulsion 0.5mL/ only, afternoon gastric infusion, continuous 10d.Dosage is same
Experiment one, before last dose mouse fasting can't help water 12h, 1h mouse pluck eyeball and take blood (testing 11d) after last dose,
3000r/min centrifuges 5min, separates serum, illustrates to carry out serum TC, TG measure according to kit.It the results are shown in Table 3.
The influence horizontal to hyperlipidemia model lipid of mice caused by high lipoprotein emulsion of table 3
Note：Compared with model group, * * P<0.01；*P<0.05；Sample measurements=sample measured value/control group measured value
Average value × 100%, TC, TG be as the relative value obtained by each measured value/control cell mean, the way is to eliminate not
With animal between batch and the otherness of reagent, make result more accurate.
The result of table 3 shows that the high lipoprotein emulsion 10d of gavage can cause model mice TG, TC notable rise, illustrate modeling into
Work(.TC, TG that radix fici simplicissimae group, ganoderma lucidum and Radix Angelicae Sinensis can not be reduced substantially when being used alone in model mice serum are horizontal, the five fingers
After wild peach group, ganoderma lucidum and Radix Angelicae Sinensis combination of two compatibility, there is reduction trend to TC, TG in mice serum, but anticipate without statistics
Justice.After radix fici simplicissimae group, ganoderma lucidum and Radix Angelicae Sinensis composition compatibility, then TC, the TG that can significantly reduce in mice serum are horizontal,
Test result indicates that pharmaceutical composition of the present invention has the function that reducing blood lipid.
Claims (10)
- A kind of 1. pharmaceutical composition for being used to improve immune function of human body, it is characterised in that：It is by following components and weight ratio Raw material composition：The parts by weight of radix fici simplicissimae 8~80, the parts by weight of Radix Angelicae Sinensis 5~60, the parts by weight of ganoderma lucidum 1~30.
- A kind of 2. pharmaceutical composition for being used to improve immune function of human body according to claim 1, it is characterised in that：The five fingers The parts by weight of wild peach 10~60, the parts by weight of Radix Angelicae Sinensis 8~50, the parts by weight of ganoderma lucidum 3~25.
- A kind of 3. pharmaceutical composition for being used to improve immune function of human body according to claim 1, it is characterised in that：The five fingers The parts by weight of wild peach 15~40, the parts by weight of Radix Angelicae Sinensis 10~40, the parts by weight of ganoderma lucidum 5~20.
- A kind of 4. pharmaceutical composition for being used to improve immune function of human body according to claim 1, it is characterised in that：The five fingers The parts by weight of wild peach 20~35, the parts by weight of Radix Angelicae Sinensis 15~30, the parts by weight of ganoderma lucidum 8~15.
- A kind of 5. pharmaceutical composition for being used to improve immune function of human body according to claim 1, it is characterised in that：The five fingers The parts by weight of wild peach 30, the parts by weight of Radix Angelicae Sinensis 20, the parts by weight of ganoderma lucidum 10.
- A kind of 6. pharmaceutical composition for being used to improve immune function of human body, it is characterised in that：It is by following components and weight ratio Raw material composition：The parts by weight of radix fici simplicissimatis extractive 8~80, the parts by weight of angelica extract 5~60, the weight of Ganodenna Lucidum P.E 1~30 Measure part.
- A kind of 7. pharmaceutical composition for being used to improve immune function of human body according to claim 1, it is characterised in that：The five fingers The parts by weight of wild peach extract 30, the parts by weight of angelica extract 20, the parts by weight of Ganodenna Lucidum P.E 10.
- A kind of 8. system for being used to improve the pharmaceutical composition of immune function of human body as described in any one in claim 1~7 Preparation Method, it is characterised in that：It comprises the following steps：S1：Weigh raw material：Radix fici simplicissimae, Radix Angelicae Sinensis and ganoderma lucidum are weighed by said components and weight ratio；S2：After raw material is well mixed, adds pharmaceutically acceptable auxiliary material and be prepared into pharmaceutically conventional pharmaceutical preparation.
- 9. a kind of preparation method for being used to improve the pharmaceutical composition of immune function of human body according to claim 8, it is special Sign is：Described pharmaceutical preparation is tablet, capsule, granule, powder, oral liquid, freeze-dried powder or parenteral solution.
- 10. the pharmaceutical composition as described in any one claim in claim 1~7 is being prepared for improving human immunity Application in function medicament.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710724860.3A CN107334818A (en) | 2017-08-22 | 2017-08-22 | A kind of pharmaceutical composition and preparation method and purposes for being used to improve immune function of human body |
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| CN201710724860.3A CN107334818A (en) | 2017-08-22 | 2017-08-22 | A kind of pharmaceutical composition and preparation method and purposes for being used to improve immune function of human body |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108308496A (en) * | 2018-02-05 | 2018-07-24 | 河源市金源绿色生命有限公司 | A kind of herbaceous plant beverage and preparation method thereof for being conducive to improve immunity |
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| CN108606996A (en) * | 2016-12-12 | 2018-10-02 | 冯正美 | It is a kind of that there is the pharmaceutical composition for improving immune function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108606996A (en) * | 2016-12-12 | 2018-10-02 | 冯正美 | It is a kind of that there is the pharmaceutical composition for improving immune function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108308496A (en) * | 2018-02-05 | 2018-07-24 | 河源市金源绿色生命有限公司 | A kind of herbaceous plant beverage and preparation method thereof for being conducive to improve immunity |
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Application publication date: 20171110 |