CN107334780B

CN107334780B - Application of fucoidin in controlling orthodontic tooth movement

Info

Publication number: CN107334780B
Application number: CN201710505828.6A
Authority: CN
Inventors: 严斌; 张瀚文; 王思雨; 王燕; 朱琳琳
Original assignee: Affiliated Stomatological Hospital of Nanjing Medical University
Current assignee: Affiliated Stomatological Hospital of Nanjing Medical University
Priority date: 2017-06-28
Filing date: 2017-06-28
Publication date: 2020-05-08
Anticipated expiration: 2037-06-28
Also published as: CN107334780A

Abstract

The invention discloses application of fucoidin in orthodontic treatment. The fucoidin provided by the invention can inhibit osteoclast formation, increase osteoblast formation and enhance orthodontic anchorage; after orthodontic operation, the matching can reduce the holding time, increase osteogenesis, enable the tooth position to be more stable and reduce recurrence.

Description

Application of fucoidin in controlling orthodontic tooth movement

Technical Field

The invention relates to the technical field of medicines, in particular to application of fucoidin in controlling orthodontic tooth movement.

Background

At present, orthodontists are often plagued by problems of patient compliance, the maintenance of anchorage, and the lack of anchorage units, primarily due to the lack of effective anchorage devices. The use of an extra-headgear arch, a device for controlling anchorage that requires patient coordination, in orthodontic treatment has been in use for at least a hundred years. Although used for a long time, the effect it produces is only generally applicable. In recent years, with the introduction of temporary anchorage devices, the prospects of patient compliance, anchorage protection, and simplified treatment of complex deformities have changed. However, as occurred with each revolution, the learning curve of the temporary anchorage device is correspondingly deepened due to the lack of prospective findings based on evidence of its associated stability, clinical application, and long-term prognosis. The use of this technique is limited because it is still young and the treatment results are short-lived and invasive to the patient.

At the same time, long term stabilization is an important goal in orthodontics. Research shows that the difference of the bone density of alveolar bones at intervals of the same alveolar is significant before and after treatment, a retainer needs to be worn after orthodontic treatment to stabilize teeth at the corrected position, the retainer is generally worn for at least 1-2 years, and some malocclusion patients even need to wear the retainer for the whole life, so that great inconvenience is brought to the patients, and the appearance and the function are influenced.

Fucoidan (Fucoidan), also known as Fucoidan sulfate, is a unique water-soluble polysaccharide combined with sulfate groups, is obtained by esterifying Fucoidan with sulfate groups, and is mainly present in brown algae and some marine invertebrates. A large number of scientific researches prove that the fucoidin has the physiological effects of regulating the immunity in two ways, eliminating free radicals, resisting aging, blood coagulation and thrombus, resisting tumor and HIV virus, eliminating gastrointestinal system disorder, resisting allergy, enhancing the liver function, reducing hyperlipidemia and hypertension, stabilizing the blood sugar level, promoting the skin regeneration, moisturizing the skin and the like, and has more than 20 items.

Disclosure of Invention

In order to solve the problems, the fucoidin provided by the invention can inhibit osteoclast formation, increase osteoblast formation and enhance orthodontic anchorage; after orthodontic operation, the matching can reduce the holding time, increase osteogenesis, enable the tooth position to be more stable and reduce recurrence.

The invention provides an application of fucoidin in preparing a medicine for enhancing orthodontic anchorage.

The invention provides an application of fucoidin in preparing a medicine for a retention stage after orthodontic treatment.

The invention can inhibit osteoclast formation and increase osteoblast formation, is beneficial to enhancing local bone anchorage, reduces tooth movement, has no invasive injury, and is easy to be accepted by patients; after orthodontic operation, the invention can reduce the holding time, increase osteogenesis, enable the tooth position to be more stable and reduce recurrence.

The invention can be locally placed in gingival crevicular fluid of the anchorage tooth or attached to the gingival surface of the local anchorage tooth clinically, and effective medicinal ingredients contained in the invention can penetrate into periodontal tissues, thereby playing the roles of slowing tooth movement and enhancing anchorage.

The polysaccharide component contained in the polysaccharide composition has a plurality of benefits for human bodies, such as antianaphylaxis, anti-tumor, anti-obesity, anti-diabetes, anticoagulation, antivirus, immunomodulator, cardioprotection, anti-liver disease, anti-neuropathy, anti-adrenal gland activity and the like, has small toxic and side effects, and can be taken for a long time.

Drawings

Fig. 1 is a schematic diagram of an orthodontic model established in the mouth of a rat.

Fig. 2 is a photograph of rats in which orthodontic models were established.

Fig. 3 is a photograph of rats in which orthodontic models were established.

FIG. 4 is a photograph of scanning Micro-CT after 1 week of orthodontic model establishment in experimental rats.

FIG. 5 is a photograph of a control rat scanning Micro-CT 1 week after orthodontic modeling.

Fig. 6 is a scatter diagram of the tooth movement distance of rats 1 week after the orthodontic model is established.

FIG. 7 is a photograph of scanning Micro-CT 2 weeks after the experimental rats had established an orthodontic model.

FIG. 8 is a photograph of scanning Micro-CT after 2 weeks of orthodontic model establishment in control rats.

Fig. 9 is a scatter plot of the tooth movement distance of rats 2 weeks after the orthodontic model was established.

FIG. 10 is a photograph of scanning Micro-CT 3 weeks after the experimental rats had established an orthodontic model.

FIG. 11 is a photograph of a scan of Micro-CT 3 weeks after the control rats were established in an orthodontic model.

Fig. 12 is a scatter plot of the tooth movement distance of rats 3 weeks after the establishment of the orthodontic model.

FIG. 13 is a line graph showing the tooth movement distance of rats 1-3 weeks after the establishment of an orthodontic model.

Detailed Description

The technical solution of the present invention will be described in detail below with reference to specific examples.

Examples

6 male wistar rats weighing 220 + -10 g were selected and randomly assigned to obtain experimental and control groups.

The experimental group is intraperitoneally injected with fucoidan (50mg/kg, 5mg/ml) every day, and the control group is intraperitoneally injected with normal saline with the same volume as the control group every day; injections were repeated daily at the same time points to maintain blood levels.

1w later experiment group and control group establish orthodontics model, incisor in upper jaw uses resin as crown as anchorage; the first molar on the left side of the upper jaw is a moving tooth, a nickel-titanium tension spring is used for applying force, and the force value of the dynamometer is 50 g. As shown in fig. 1, 2 and 3, fig. 1 is a schematic view of the orthodontic model established in the oral cavity of a mouse, and fig. 2 and 3 are photographs of the orthodontic model established in the oral cavity of the mouse.

Scanning Micro-CT (Micro-computed tomogry) before, after, 1w after, 2w after, and 3w after the operation, measuring the change of the moving distance of the teeth, and performing statistical analysis, as shown in fig. 4-13, wherein fig. 4, 7, and 10 sequentially set up orthodontic models for rats in the experimental group, and scan photos of the Micro-CT after 1 week, 2 weeks, and 3 weeks; FIG. 5, FIG. 8 and FIG. 11 are photographs of scanning Micro-CT after establishing orthodontic model for control rats for 1 week, 2 weeks and 3 weeks in sequence; fig. 6, 9 and 12 are scatter diagrams of the tooth movement distance of the rat after 1 week, 2 weeks and 3 weeks of the establishment of the orthodontic model in sequence; FIG. 13 is a line graph showing the tooth movement distance of rats 1-3 weeks after the establishment of an orthodontic model.

The results show that: after 1-3 weeks of orthodontic model establishment, the tooth moving distance of the control group is larger than that of the experimental group, and the differences have statistical significance (P is less than 0.05).

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. An application of fucoidin in preparing medicine for enhancing orthodontic anchorage is disclosed.

2. Use of fucoidan for preparing a medicament for use in the post-orthodontic retention stage.