CN107333940A - Burdock functional red yeast rice health protection tea and preparation method thereof - Google Patents
Burdock functional red yeast rice health protection tea and preparation method thereof Download PDFInfo
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- A23F3/00—Tea; Tea substitutes; Preparations thereof
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Abstract
The invention belongs to beverage production field, and in particular to a kind of preparation method of burdock functional red yeast rice health protection tea.The functional red yeast rice that the health protection tea uses for put into practice No. ten Flight Mutagenesis mutant strain monascuses (Monascus sp.), its microbial preservation numbering is：CGMCC NO.13773.Above-mentioned functional red yeast rice strain is inoculated in the material containing burdock, ferments, obtains burdock functional red yeast rice health protection tea.The present invention using burdock as main fermentation substrate, by functional red yeast rice be metabolized caused by physiological activator, such as Mo Nakelin K, gamma aminobutyric acid, be combined with the abundant nutrition material that burdock contains, the product of the present invention is possessed higher healthy nutritive value.
Description
Technical field
The invention belongs to beverage production field, and in particular to a kind of preparation method of burdock functional red yeast rice health protection tea.
Background technology
Red yeast rice is the great invention of Ancient Times in China, is referred to as red bent.The Ming Dynasty《Compendium of Materia Medica》Described in：" red yeast rice, it is warm-natured, sweet, disappear
Food promoting blood circulation, spleen and stomach dry, wine brewing, blood-breaking row medicine gesture." record after the Ming Dynasty on red yeast rice increasingly increases, red yeast rice is applied
It is among the people also more and more extensive, but have focused largely on traditional wine brewing, vinegar processed, colouring agent etc..Until the remote rattan chapter of Japan from
Discovery and a kind of isolated active material Mo Nakelin K in Monas cuspurpureus Went fermentation liquid（Monacolin K）, such material is HMG-
CoA reductase inhibitors, the synthesis of internal cholesterol can be significantly inhibited, in addition, red yeast rice metabolism caused by other functions because
Son, such as γ-aminobutyric acid, significant effect can also be played to hypotensive.Thus, researcher both domestic and external starts to day
Right red yeast rice and its spit of fland class material of metabolism give extensive concern, have even more started the upsurge of exploitation monascus health product.
Functional red yeast rice fermentation is presently mainly that the matrix of monascus traditional zymotic is rice by the way of solid fermentation.
Rice is higher as a kind of main cereal crops, cost.Therefore, exploitation one kind can be made full use of by red yeast rice, and can be with
Further increasing the novel fermentation matrix of red koji fermentation thing nutritive value turns into study hotspot.
Burdock, also known as：Great Burdock Achene, white muscle ginseng etc..It is the biennial large-scale herbaceous plant of the Compositae Arctium lappa category system of taproot, according to
《Dictionary of medicinal plant》Record, burdock is warm-natured, sweet, nontoxic, there is clearing heat and detoxicating, clearing damp, spleen benefiting and stimulating the appetite, defaecation, enriching yin and nourishing kidney, benefit
The effect of gas.Contain synanthrin, volatile oil, arctic acid, a variety of polyphenol substances and aldehydes in radix bardanae, and be rich in vitamin, meals
Fiber, the content of its carrotene occupy second in vegetables, and the content of protein and calcium is that highest is a kind of in root vegetable,
The epidermis of radix bardanae also containing chlorogenic acid, isochlorogenic acid, caffeic acid isoreactivity material, also has with this radix bardanae and removes oxygen freedom
Base, oxidation resistant activity.As can be seen here, burdock is a kind of preferable medicinal and edible plant.
At present in the Jiangsu Province in China and Shandong Province, Xuzhou Feng County, the Pei County in Jiangsu Province, the Cangshan plantation history of Shandong Province
Long, area is larger, and the gross area reaches 2.33 ten thousand hectares, and the cultivated area of Xuzhou Feng County is just reachable 10,000 hectares, the Shandong Weihe River
Mill cultivated area reaches 0.67 ten thousand hectares, and the cultivation of burdock is also being promoted in Liaoning some areas.
Contain the function factors such as substantial amounts of synanthrin and water-soluble dietary fiber in burdock.Synanthrin is also known as inulin, is a kind of day
Right water miscible levulan, source is mainly feverfew, and synanthrin has indigestion and fermentability, body is remained normal
Enteron aisle ecology, can by enteron aisle harmful substance dilute so as to prevent the generation of Cancerous disease, in addition, synanthrin is preventing
Diabetes, norcholesterol etc. have the effect of certain.Therefore, burdock tea has important to hypertension, diabetes, constipation etc.
Food therapy effect.
Therefore, if burdock is applied into tea beverage, contain in bioactive substance and burdock caused by functional red yeast rice metabolism
Some function factors make tea beverage have certain medical value, and this is extremely significant to health.
The content of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of burdock functional red yeast rice with multiple functions activity
Health protection tea.
The preparation method of burdock functional red yeast rice health protection tea, the health protection tea are sent out using burdock as primary raw material using functional red yeast rice
Ferment is made,
The functional red yeast rice that the health protection tea uses for put into practice No. ten Flight Mutagenesis mutant strain monascuses (Monascus sp.), its is micro-
Biological deposits are numbered：CGMCC NO.13773, No. 3 China of institute of Chaoyang District North Star West Road 1 are preserved on April 11st, 2017
Institute of microbiology of the academy of sciences, China General Microbiological culture presevation administrative center, monascus (Monascus sp.) be in and deposit
State living.
By the functional red yeast rice strain monascus of above-mentioned preservation (Monascus sp.) be inoculated in the material containing burdock,
Fermentation, obtains burdock functional red yeast rice health protection tea.
The preparation method of the burdock functional red yeast rice health protection tea of the present invention, including following steps：
Cleaning, dries, and crushes, dispensing, sterilizing, is inoculated with functional red yeast rice strain, fermented and cultured, obtains burdock functional red yeast rice health protection tea.
Specifically, the preparation method of burdock functional red yeast rice health protection tea, including following steps：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, is dried, crushes, obtains burdock root powder；
S2：Dispensing：Burdock root powder 40-80, fructose 10-20, glycerine 1-4, peptone 0.2-2, wheat bran are taken by following parts by weight
5-15, it is gained burdock root powder in S1 to adjust and burdock root powder is taken in pH 3-4, S2；
S3：Material in sterilizing S2；
S4：Liquid functional red yeast rice strain is accessed, fermented and cultured, untill Lovastatin content is 2.5mg/g, obtains burdock function
Red koji fermentation thing, fermentate is dried, and is crushed, is obtained burdock functional red yeast rice health protection tea.
The preparation method of burdock functional red yeast rice health protection tea, including following steps：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 80-100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 40-80, fructose 10-20, glycerine 1-4, peptone 0.2-2, wheat bran are taken by following parts by weight
5-15, it is gained burdock root powder in S1 to adjust and burdock root powder is taken in pH 3-4, S2；
S3：Material in sterilizing S2；
S4：Liquid functional red yeast rice strain is accessed, plants 48 hours, inoculum concentration 5-15% age, fermented and cultured 12-25 days, to Lovastatin
Untill content is 2.5mg/g, burdock functional red yeast rice fermentate is obtained, fermentate is dried, ultramicro grinding is gone out to 600-1000 mesh
Bacterium, obtain burdock functional red yeast rice health protection tea.
Preferably, in S3, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C.
Wherein, the cultural method of liquid functional red yeast rice strain is as follows：
（1）By the culture of monascus specie test tube slant, spore suspension is made after activation；
（2）It is fitted into after steaming long-grained nonglutinous rice in triangular flask, sterilizes；
（3）Will（1）In inoculation of suspension liquid in（2）In material after middle sterilizing, culture；
（4）Select（3）In the grain of rice, put into practice No. ten recoverable spacecrafts by satellite launch center's carrying and enter space flight；
（5）It is right（4）Primary dcreening operation is carried out after the grain of rice activation by mutagenesis of middle return, picks out objective function red yeast rice single bacterium colony, it is pure
Change culture of isolated, inclined-plane culture to maturation, obtain liquid functional red yeast rice strain.
The preparation method of burdock functional red yeast rice health protection tea, including following steps：
（1）By the culture of monascus specie test tube slant, spore suspension is made after activation；
Slant medium：Maltose 1-10%, peptone 1-10%, agar powder 1-5%, soluble starch 1-10%；
Cultivated 5-10 days under the conditions of 20-35 DEG C of test tube slant after inoculation, suspension is made；
（2）It is fitted into after steaming long-grained nonglutinous rice in triangular flask, sterilizes；
（3）Will（1）In inoculation of suspension liquid in（2）In material after middle sterilizing, culture；
（4）Select（3）In the grain of rice, put into practice No. ten recoverable spacecrafts by satellite launch center's carrying and enter space flight；
（5）It is right（4）The grain of rice by mutagenesis of middle return is ground to powdered, and sterilized water mixed, and 20-50 DEG C of water-bath is gone out miscellaneous bacteria
1-4h, divide mixed liquor to gradient dilution, apply flat board, divide gradient plate culture, pick out functional red yeast rice single bacterium colony；
At least fermenting experiment checking three times, fermenting experiment medium component will be passed through after the bacterium colony culture selected：Cellulose 40-
85%, glycerine 1-10%, bean powder 1-10%, peptone 0.1-2%, wheat bran 5-15%, corn steep liquor 0.1-1%, pH3-4 is adjusted with lactic acid,
It is weight percentage above；
Sterilizing：1kg/cm2Sterilized 20 minutes at 121 DEG C；
Inoculation：The various high-cellulose adaptive functional aspergillus kind seed liquors filtered out are respectively connected to, plant 28 hours ages, inoculum concentration
5-15%；
Fermented and cultured 20-30 days；Continuously above-mentioned experiment is repeated three times, it is final to determine objective function aspergillus kind, for burdock function
Koji fermentation is inoculated with.
The beneficial effects of the present invention are：
1. the present invention using burdock as main fermentation substrate, by functional red yeast rice be metabolized caused by physiological activator, Ru Mona
Crin K, gamma aminobutyric acid etc., it is combined with the abundant nutrition material that burdock contains, possesses product of the invention higher
Healthy nutritive value；
2. the present invention uses burdock and fructose, for functional red yeast rice fermenting carbon source, burdock sugar content is relatively low, although in addition, fructose easily quilt
Absorption of human body, but it is small to blood sugar influence, independent of insulin, it is adapted to the intake of diabetic；
3. because sugar content is relatively low in burdock, the carbon source provided in fermentation process is limited, therefore the present invention passes through Flight Mutagenesis
Technology carries out mutagenesis to function aspergillus kind, and successfully filters out one plant of low sugar functional red yeast rice strain, in the relatively low feelings of carbon source content
Also normal metabolite accumulation can be carried out under condition；
4. the present invention carries out post-production using superfine communication technique to burdock functional red yeast rice fermentate, it is set to be more suitable for people
Drink.Burdock functional red yeast rice health protection tea has the effect of reducing blood lipid, hypotensive, prevention sun damage and light aging, dust-collection sterilization,
The health-care effect of functional red yeast rice is not only embodied, also highlights the healthy nutritive value of burdock.
Brief description of the drawings
Fig. 1 is Lovastatin content detection chromatogram in the product of embodiment 1；
Fig. 2 is that the Lovastatin content in embodiment 1 changes over time figure；
Fig. 3 is that the Lovastatin content in embodiment 2 changes over time figure；
Fig. 4 is that the Lovastatin content in embodiment 3 changes over time figure.
Embodiment
The present invention is further described with reference to specific embodiment, so that those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 60 is taken by following parts by weight, fructose 15, glycerine 2, peptone 0.8, wheat bran 10, adjusts pH
It is gained burdock root powder in S1 that burdock root powder is taken in 3.5, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Liquid functional red yeast rice strain is accessed, is planted 48 hours ages, inoculum concentration 10%, fermented and cultured 15 days, is detected in fermentation materials
Lovastatin content is 2.5mg/g, now stops fermentation, obtains burdock functional red yeast rice fermentate, fermentate is dried, Ultramicro-powder
800 mesh are broken to, are sterilized, pack, packaging, obtain burdock functional red yeast rice health protection tea.
The preparation method of liquid functional red yeast rice strain is as follows：
（1）By the culture of monascus specie test tube slant, spore suspension is made after activation；
Slant medium：Maltose 6%, peptone 8%, agar powder 3%, soluble starch 5%；
Cultivated 8 days under the conditions of 25 DEG C of test tube slant after inoculation, preservation under the conditions of 4 DEG C of slant strains for cultivating completion is carried out
Need to activate 20h under the conditions of 25 DEG C of the strain by preservation before experiment, then need not if cultured strain is directly used in into experiment
Activated, suspension is directly made；
（2）It is fitted into after steaming long-grained nonglutinous rice in triangular flask, sterilizes, condition is, in 1kg/cm2And sterilized 20 minutes at a temperature of 121 DEG C；
（3）Will（1）In inoculation of suspension liquid in（2）In material after middle sterilizing, culture；
（4）Select（3）In the grain of rice, put into practice No. ten recoverable spacecrafts by satellite launch center's carrying and enter space flight；
（5）It is right（4）The grain of rice by mutagenesis of middle return be ground to it is powdered, in test tube and sterilized water mix, 40 DEG C of water-baths
Go out miscellaneous bacteria 3h, divides mixed liquor to gradient dilution, applies flat board, divide gradient plate culture, observes bacterium colony in different gradient medias and gives birth to
Long situation, the clump count in identical dilution gradient flat board being counted, pick out cellulose adaptability height, eccrine fiber element enzyme is vigorous,
Bacterium colony fold is obvious, aerial hyphae is abundant, colonial morphology is larger, the high single bacterium colony of clump count；
By, by the way that at least fermenting experiment is verified three times, verification step is as follows after the bacterium colony culture selected：
Fermenting experiment medium component：Cellulose 60%, glycerine 7%, bean powder 6%, peptone 0.8%, wheat bran 10%, corn steep liquor
0.6%, pH3.5 is adjusted with lactic acid, the above is weight percentage；
Sterilizing：1kg/cm2Sterilized 20 minutes at 121 DEG C；
Inoculation：The various high-cellulose adaptive functional aspergillus kind seed liquors filtered out are respectively connected to, plant 28 hours ages, inoculum concentration
5-15%；
Fermented and cultured 25 days or so, fermentation situation, microscopy mycelia sturdy bacterium fast with mycelial growth rate are observed in experimentation
Kind is target strain, finally determines preferable strain fermentation thing Lovastatin content；
Continuously above-mentioned experiment is repeated three times, it is final to determine objective function aspergillus kind, select a plant height cellulose adaptability, heredity steady
Qualitative function aspergillus kind high, production Lovastatin ability is strong, is inoculated with for burdock function koji fermentation.
The functional red yeast rice of the present invention puts into practice No. ten and enters space by Chinese Jiuquan Satellite Launch Center, carrying, puts into practice ten
Number recoverable scientific experiment satellite 38 divided and transported in Chinese Jiuquan Satellite Launch Center by CZ-2D when 6 days 1 April in 2016
Rocket launching lift-off is carried, is run by the tracks of 12 days, No. ten recoverable scientific experiment satellites is put into practice and returns, in 2016 4
30 divide recovery of being landed in Inner Mongolia Autonomous Region during the moon 18 days 16.The present inventor will pass through the functional red yeast rice bacterium of above-mentioned Seeds of First Post-flight
Kind is notarized in circumference notarial office of Beijing, and the micro- of applicant's carrying is taken out in the carrying packaging bag for being 2-2 to numbering
Biological species check, have confirmed, totally a kind of the microbial species that Shandong Zhong Hui biotech inc is carried, gross weight
Totally 1 gram.Notarial office is proved to put into practice the carrying microbial species that are taken out when No. ten recoverable scientific experiment satellite return capsules open the cabin
Packaging bag envelope is all right, and strip of paper used for sealing stands intact, and it is micro- to demonstrate 1 gram sealed up for safekeeping in the exhibit bag of notarization phase adhesion
Biological species, the carrying packaging bag that numbering is 2-2 is taken from, to put into practice one of No. ten recoverable scientific experiment Seeds of First Post-flight things.
The functional red yeast rice that health protection tea in embodiment 1 uses is puts into practice No. ten Flight Mutagenesis mutant strain monascuses
(Monascus sp.), its microbial preservation numbering is：CGMCC NO.13773, Chaoyang District is preserved on April 11st, 2017
No. 3 Institute of Microorganism, Academia Sinica of institute of North Star West Road 1, China General Microbiological culture presevation administrative center, monascus
(Monascus sp.) it is in existing state.
Comparative example 1
Inoculum concentration is reduced to 3%, ferment effect is investigated, carries out one group of contrast experiment：
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 60 is taken by following parts by weight, fructose 15, glycerine 2, peptone 0.8, wheat bran 10, adjusts pH
It is gained burdock root powder in S1 that burdock root powder is taken in 3.5, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Liquid functional red yeast rice strain is accessed, is planted 48 hours ages, inoculum concentration 3%, fermented and cultured 15 days, detects Lip river in fermentation materials
Liprevil content is 0.4mg/g, now stops fermentation, obtains burdock functional red yeast rice fermentate, fermentate is dried, ultramicro grinding
To 800 mesh, sterilizing, pack, packaging, burdock functional red yeast rice health protection tea is obtained.
The preparation method of liquid functional red yeast rice strain is the same as red using identical liquid functional in embodiment 1, with embodiment 1
It is bent.Liquid functional red yeast rice employed in following comparative example 2 is same.
Comparative example 2
Extend fermentation time, investigate ferment effect, carry out one group of contrast experiment：
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 60 is taken by following parts by weight, fructose 15, glycerine 2, peptone 0.8, wheat bran 10, adjusts pH
It is gained burdock root powder in S1 that burdock root powder is taken in 3.5, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Liquid functional red yeast rice strain is accessed, is planted 48 hours ages, inoculum concentration 3%, fermented and cultured 25 days, detects Lip river in fermentation materials
Liprevil content is 1.1mg/g, now stops fermentation, obtains burdock functional red yeast rice fermentate, fermentate is dried, ultramicro grinding
To 800 mesh, sterilizing, pack, packaging, burdock functional red yeast rice health protection tea is obtained.
Comparative example 3
Using commercially available liquid functional red yeast rice come the liquid functional red yeast rice in alternate embodiment 1, it is as follows to investigate its effect：
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 60 is taken by following parts by weight, fructose 15, glycerine 2, peptone 0.8, wheat bran 10, adjusts pH
It is gained burdock root powder in S1 that burdock root powder is taken in 3.5, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Commercially available liquid functional red yeast rice strain is accessed, 48 hours ages is planted, inoculum concentration 10%, fermented and cultured 15 days, now stops
Fermentation, obtains burdock functional red yeast rice fermentate, fermentate is dried, ultramicro grinding to 800 mesh, sterilizing, pack, packaging, obtains burdock
Functional red yeast rice health protection tea.
The influence that culture medium on different liquid functional red yeast rice strains produces to Lovastatin in red yeast rice, i.e., " will choose
By the way that at least fermenting experiment is verified three times after the bacterium colony culture of choosing ", in the step, cultivate the influence for whole product, this hair
A person of good sense has carried out following experiment：
Comparative example 4-7 changes fermentation medium components：
Embodiment 4-7 other steps are only that the excellent bacterium colony selected is cultivated using different culture mediums with embodiment 1,
After fermentation condition fermentation same as Example 1, the content of Lovastatin in product is measured as shown in below table：
Embodiment 2
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 80 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 40-80, fructose 10-20, glycerine 1-4, peptone 0.2-2, wheat bran are taken by following parts by weight
5-15, it is gained burdock root powder in S1 to adjust and burdock root powder is taken in pH 3-4, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Liquid functional red yeast rice strain is accessed, 48 hours ages is planted, inoculum concentration 8%, fermented and cultured 25 days, is to Lovastatin content
Untill 2.5mg/g, burdock functional red yeast rice fermentate is obtained, fermentate is dried, ultramicro grinding to 800 mesh, sterilizing, pack, bag
Dress, obtains burdock functional red yeast rice health protection tea.The preparation method of liquid functional red yeast rice strain is same as Example 1.
Embodiment 3
A kind of preparation method of burdock functional red yeast rice health protection tea, its step are as follows：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 80 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Burdock root powder 40-80, fructose 10-20, glycerine 1-4, peptone 0.2-2, wheat bran are taken by following parts by weight
5-15, it is gained burdock root powder in S1 to adjust and burdock root powder is taken in pH 3-4, S2；
S3：Material in sterilizing S2, sterilization steps are in 1kg/cm2, sterilize 20 minutes at 121 DEG C；
S4：Liquid functional red yeast rice strain is accessed, plants 48 hours ages, inoculum concentration 10%, fermented and cultured 15 days, to Lovastatin content
Untill for 2.5mg/g, burdock functional red yeast rice fermentate is obtained, fermentate is dried, ultramicro grinding to 1000 mesh, sterilizing, pack,
Packaging, obtains burdock functional red yeast rice health protection tea.The preparation method of liquid functional red yeast rice strain is same as Example 1.
Embodiment 4
The health red rice tea of gained in Example 1, determines the content of Lovastatin therein, and specific detection method is as follows：
（1）Sample pretreating：Burdock functional Monascus is crushed and is sufficiently mixed uniformly.Accurately 0.1-0.5g samples are weighed in 50ml
In volumetric flask.75% ethanol is added to close to graduation mark, then ultrasonic 5-15min, room temperature is cooled to afterwards, is settled to 75% ethanol
50ml.5-15min is centrifuged with 2000-4000r/min rotary speed.Supernatant is removed through 0.2-0.5 μm of micro porous filtration membrane filtration,
Filtrate is stand-by.
（2）Liquid-phase chromatographic column reference conditions
Chromatographic column：C18 posts
Column temperature：20-25 DEG C of room temperature
UV-detector：238nm Detection wavelengths
Mobile phase：Methanol：Water：Phosphoric acid=（300-400）:（100-120）:（0.1-0.2）
Flow velocity:0.5-1.5ml/min
Sample size：20μl
（3）It is prepared by standard curve
Compound concentration is 0.1,1,10,30,75,150,300 μ g/ml Lovastatin standard liquids.During analysis, balanced with eluent
Analytical column, the Lovastatin titer of various concentrations is subjected to HPLC analyses after baseline stability, determines peak area, using peak area as
Ordinate, using Lovastatin content as abscissa, linear relationship is good, and subsequent sample measure is carried out when r is more than 0.9995.
（4）Chromatography
The μ l sample introductions of sample extracting solution 20 that will be handled well, compareed with standard liquid retention time it is qualitative, with tested component lactones and
Acid Lovastatin peak area sum and standard Lovastatin（Lactones）The ratio between peak area quantified.
（5）As a result calculate
X=（H1+H2）×C×50/（H3×m）
In formula：Mo Nakelin K contents in X- samples, unit mg/g；
Lactones formula Lovastatin area in H1- samples；
Acid Lovastatin peak area in H2- samples；
C- standard Lovastatins（Lactones）Solution concentration, units/ml；
50- sample constant volumes
H3- standard Lovastatins（Lactones）Solution peak area；
The M- sample amounts of weighing, unit g.
Method more than, it is 2.5mg/g to detect the Lovastatin content in embodiment 1.
Claims (8)
1. the preparation method of burdock functional red yeast rice health protection tea, the functional red yeast rice that the health protection tea uses is dashed forward to put into practice No. ten Flight Mutagenesis
Change bacterial strain monascus (Monascus sp.), its microbial preservation numbering is：CGMCC NO.13773.
2. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, it is characterised in that by functional red yeast rice strain
Monascus (Monascus sp.) be inoculated in the material containing burdock, ferment, obtain burdock functional red yeast rice health protection tea.
3. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, including following steps：
Cleaning, dries, and crushes, dispensing, sterilizing, is inoculated with functional red yeast rice strain, fermented and cultured, obtains burdock functional red yeast rice health protection tea.
4. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, including following steps：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, is dried, crushes, obtains burdock root powder；
S2：Dispensing：Each raw material is taken by following parts by weight：Burdock root powder 40-80, fructose 10-20, the glycerine 1-4 of gained in S1,
Peptone 0.2-2, wheat bran 5-15, adjust pH 3-4；
S3：Material in sterilizing S2；
S4：Liquid functional red yeast rice strain is accessed, fermented and cultured, burdock functional red yeast rice fermentate is obtained, fermentate is dried, is crushed,
Obtain burdock functional red yeast rice health protection tea.
5. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, including following steps：
S1:Burdock pre-processes：Radix bardanae is cleaned, section, naturally dry, 80-100 mesh is crushed to, obtains burdock root powder；
S2：Dispensing：Each raw material is taken by following parts by weight：Burdock root powder 40-80, fructose 10-20, the glycerine 1-4 of gained in S1,
Peptone 0.2-2, wheat bran 5-15, adjust pH 3-4；
S3：Material in sterilizing S2；
S4：Liquid functional red yeast rice strain is accessed, 48 hours ages is planted, inoculum concentration 5-15%, fermented and cultured 12-25 days, obtains burdock function
Red koji fermentation thing, fermentate is dried, ultramicro grinding to 600-1000 mesh, sterilizing, obtains burdock functional red yeast rice health protection tea.
6. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, it is characterised in that in S3, sterilization steps
It is in 1kg/cm2, sterilize 20 minutes at 121 DEG C.
7. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, it is characterised in that liquid functional Monascus
The cultural method of kind is as follows：
（1）By the culture of monascus specie test tube slant, spore suspension is made after activation；
（2）It is fitted into after steaming long-grained nonglutinous rice in triangular flask, sterilizes；
（3）Will（1）In inoculation of suspension liquid in（2）In material after middle sterilizing, culture；
（4）Select（3）In the grain of rice, put into practice No. ten recoverable spacecrafts by satellite launch center's carrying and enter space flight；
（5）It is right（4）Primary dcreening operation is carried out after the grain of rice activation by mutagenesis of middle return, picks out objective function red yeast rice single bacterium colony, it is pure
Change culture of isolated, inclined-plane culture to maturation, obtain liquid functional red yeast rice strain.
8. the preparation method of burdock functional red yeast rice health protection tea as claimed in claim 1, including following steps：
（1）By the culture of monascus specie test tube slant, spore suspension is made after activation；
Slant medium：Maltose 1-10%, peptone 1-10%, agar powder 1-5%, soluble starch 1-10%；
Cultivated 5-10 days under the conditions of 20-35 DEG C of test tube slant after inoculation, suspension is made；
（2）It is fitted into after steaming long-grained nonglutinous rice in triangular flask, sterilizes；
（3）Will（1）In inoculation of suspension liquid in（2）In material after middle sterilizing, culture；
（4）Select（3）In the grain of rice, put into practice No. ten recoverable spacecrafts by satellite launch center's carrying and enter space flight；
（5）It is right（4）The grain of rice by mutagenesis of middle return is ground to powdered, and sterilized water mixed, and 20-50 DEG C of water-bath is gone out miscellaneous bacteria
1-4h, divide mixed liquor to gradient dilution, apply flat board, divide gradient plate culture, pick out functional red yeast rice single bacterium colony；
At least fermenting experiment checking three times, fermenting experiment medium component will be passed through after the bacterium colony culture selected：Cellulose 40-
85%, glycerine 1-10%, bean powder 1-10%, peptone 0.1-2%, wheat bran 5-15%, corn steep liquor 0.1-1%, pH3-4 is adjusted with lactic acid,
It is weight percentage above；
Sterilizing：1kg/cm2Sterilized 20 minutes at 121 DEG C；
Inoculation：The various high-cellulose adaptive functional aspergillus kind seed liquors filtered out are respectively connected to, plant 28 hours ages, inoculum concentration
5-15%；
Fermented and cultured 20-30 days；Continuously above-mentioned experiment is repeated three times, it is final to determine objective function aspergillus kind, for burdock function
Koji fermentation is inoculated with.
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| CN109430758A (en) * | 2018-09-06 | 2019-03-08 | 哈尔滨伟平科技开发有限公司 | A kind of oral solution and preparation method thereof with auxiliary hyperglycemic function |
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