CN107318785B - Medicament for increasing wax secretion amount of fraxinus chinensis and treatment method thereof - Google Patents
Medicament for increasing wax secretion amount of fraxinus chinensis and treatment method thereof Download PDFInfo
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- 241000238631 Hexapoda Species 0.000 claims abstract description 35
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- 239000002949 juvenile hormone Substances 0.000 claims abstract description 31
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- 238000005507 spraying Methods 0.000 claims abstract description 25
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a medicament for increasing wax secretion of fraxinus chinensis and a treatment method thereof, belonging to the technical field of biology. The medicament is prepared by mixing exogenous juvenile hormone analogue and 1-20% of acetone aqueous solution by volume concentration; the concentration of exogenous juvenile hormone analog in the medicament is 2-10mg•ml‑1. When in use, the pesticide is directly sprayed on the white wax moth body, and the spraying amount is 2ul-5ul per insect. The wax insect treated by the method can improve wax secretion by more than 20 percent, provides effective test data and technical method for enlarging the production and subsequent popularization work of the white wax, and is easy to popularize and apply.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a medicament for increasing wax secretion amount of wax insect and a treatment method thereof.
Background
White wax insectEricerus pela(channels) are classified in the order Hemiptera (Hemiptera), the family Lecanidae (Coccoidea) the genus Ericerus (Hemiptera)Ericerus). The Ceriporia alba is a resource insect with great economic value, and the Ceriporia alba secreted by 2-instar male larva is a widely used pure natural polymer compound, and contains hexacosanoic acid hexacosanyl ester (C)25H51COOC26H53) The white wax is mainly produced in China, and the white wax is utilized and produced in ancient China (more than 1000 years ago), and is mainly used for candles, traditional Chinese medicines, wax printing and engraving stamping. In traditional Chinese medicine, the traditional Chinese medicine is often used as a wound healing agent, a hemostatic agent, a tranquilizer and the like, and modern traditional Chinese medicine also uses white wax for treating uterine inflammation, chronic gastritis, rheumatism and the like. The white wax has the excellent characteristics of high melting point, good gloss, stable physicochemical property, moisture resistance, lubrication, gloss and the like, and is widely applied to the industries of chemical industry, medicine, food, agriculture and the like at present. White wax productThe method has the advantages that the areas are mostly distributed in poor mountainous areas in China, the improvement of the yield of the white wax can drive local economic development, meanwhile, a large number of host plants are needed for the development of the white wax, the method plays a positive promoting role in improving the local environment, and the method has important ecological, economic and social benefits in the development of the white wax industry in China.
The life cycle of the wax insect is 1 generation in 1 year, and there are amphoterism and parthenogenesis. The male wax insect grows through nymph, pupa and adult stages, and belongs to complete metamorphosis, the nymph grows on host plants twice and transfers in a lifetime, the nymph grows out from eggs for the first time, a proper leaf is searched on the host plants, the nymph grows on the back of the leaf and is fixed in an aggregation manner, and the nymph is called as 'fixed leaf' in the production of wax; the second time is that the nymphs of 1 year enter 2 years after molting, and the nymphs of 2 years are transferred from leaves to branches to live, are intensively distributed in the middle of host plants, and secrete white wax to cover worm bodies, as shown in figure 1, the nymphs are called 'fixed poles' in white wax production. The growth, development, reproduction and other behaviors of insects are influenced by various environmental factors such as temperature, humidity, illumination and the like, wax secretion of male white wax insects comprises complex ecological and physiological problems, wax secretion and other physiological phenomena are generated in the process of adapting to the complex ecological environment, the wax secretion and other physiological phenomena are mainly influenced by humidity and illumination, and the wax secretion tendency of the white wax insects in high-humidity and low-illumination areas is presented.
At present, the white wax is produced in Yunnan, Guizhou, Sichuan, Hunan, Shaanxi and other provinces, and the annual yield is more than 1000 tons at the highest. The white wax contains 26 alkanol, 28 alkanol and other higher alkanol, and has wide application in medicine, agriculture, health product and other products, and the annual output of the white wax and related products reaches over 20 hundred million. However, the white wax is limited by the quality, variety and potential energy deficiency factors of the seed insects and the later-period management, so that the yield of the white wax in the white wax producing area generally tends to decline year by year, and the production enthusiasm of wax farmers is greatly influenced. Therefore, how to improve the yield of the white wax by a convenient, effective and low-cost medicament treatment and forest management method becomes an important technical problem for solving the development bottleneck of the white wax industry in China.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a medicament for improving wax secretion of white wax insects and a treatment method thereof. The wax insect treated by the agent can improve wax secretion by more than 20 percent, and is easy to popularize and apply.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a medicament for improving wax secretion of Ceriporia alba is prepared by mixing exogenous juvenile hormone analogue and 1% -20% acetone aqueous solution by volume concentration;
the concentration of exogenous juvenile hormone analog in the preparation is 2-10mg-1。
Further, preferably, the exogenous juvenile hormone analog is, but not limited to, serpentin ZR-512 (AltozarZR-512), methoprene (MethopreneZR-515), methoprene (KinoprenezR-777), or juvenin (JH-286 farmoplant).
Further, it is preferred that the concentration of the exogenous juvenile hormone analogue in the medicament is 2.5 mg.ml-1。
The invention also provides a treatment method for improving wax secretion of the fraxinus chinensis insect, which adopts the medicament for improving the wax secretion of the fraxinus chinensis insect to directly spray the medicament on the fraxinus chinensis insect body, wherein the spraying amount is 2ul-5 ul/insect.
Further, it is preferable that the wax insect is a 2-instar male larva.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a medicament for improving wax secretion of white wax insect and a treatment method thereof, the medicament for treating white wax insect can improve the wax secretion by more than 20 percent, provides effective test data and technical method for enlarging white wax production and subsequent popularization work, and is easy to popularize and apply.
In the research, the invention discovers that the juvenile hormone analogue with medium concentration can achieve the purpose of remarkably increasing the yield and save the investment cost, and the spraying treatment is more convenient and feasible than the drying and injection, saves the labor force and is more easily accepted by farmers in the production.
Drawings
FIG. 1 is a biological process of white wax secretion by male wax worms; wherein A is 1-instar male larva fixed leaf; b is a 2-instar male larva fixed rod; c is wax secreted by male larvae of 2 years old;
FIG. 2 is the analysis of variance of wax secretion of individuals with different concentrations of wax insect treated in two regions.
Wherein A is Yunnan Kunming area; b is the region of Szechwan Emei; the ordinate represents the wax secretion amount of the wax insect individual; p <0.05, indicating significant difference from CK; p <0.01, indicating a very significant difference from CK.
Detailed Description
The present invention will be described in further detail with reference to examples.
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The materials or equipment used are not indicated by manufacturers, and all are conventional products available by purchase.
Example 1
A medicament for increasing wax secretion amount of Ceriporia alba is prepared by mixing exogenous juvenile hormone analogue and 1% acetone aqueous solution by volume concentration;
the concentration of exogenous juvenile hormone analogue in the preparation is 2 mg.ml-1。
The exogenous juvenile hormone analogue is AltozarZR-512.
The treatment method for increasing the wax secretion amount of the fraxinus chinensis bunge in the embodiment is to adopt the agent for increasing the wax secretion amount of the fraxinus chinensis bunge in the embodiment, and directly spray the agent on fraxinus chinensis bunge bodies, wherein the spraying amount is 2 ul/body.
Example 2
A medicament for increasing wax secretion amount of Ceriporia alba is prepared by mixing exogenous juvenile hormone analogue and 20% acetone aqueous solution by volume concentration;
the concentration of exogenous juvenile hormone analogue in the preparation is 10 mg.ml-1。
The exogenous juvenile hormone analogue is MethopreneZR-515.
The treatment method for improving wax secretion of the fraxinus chinensis bunge in the embodiment is to spray the medicament for improving wax secretion of the fraxinus chinensis bunge on 2-instar male larva bodies of the fraxinus chinensis bunge directly, wherein the spraying amount is 5 ul/larva.
Example 3
A medicament for increasing wax secretion amount of Ceriporia alba is prepared by mixing exogenous juvenile hormone analogue and 10% acetone aqueous solution by volume concentration;
the concentration of exogenous juvenile hormone analogue in the preparation is 5 mg.ml-1。
The exogenous juvenile hormone analogue is KinoprenezR-777.
The treatment method for improving wax secretion of the fraxinus chinensis bunge is to adopt the agent for improving wax secretion of the fraxinus chinensis bunge in the embodiment, and directly spray the agent on 2-instar male larva bodies of the fraxinus chinensis bunge, wherein the spraying amount is 3 ul/larva.
Example 4
A medicament for increasing wax secretion amount of Ceriporia alba is prepared by mixing exogenous juvenile hormone analogue and 8% acetone aqueous solution by volume concentration;
the concentration of exogenous juvenile hormone analogue in the preparation is 2.5 mg.ml-1。
The exogenous juvenile hormone analogue is JH-286 farmoplant.
The treatment method for improving wax secretion of the fraxinus chinensis bunge is to adopt the agent for improving wax secretion of the fraxinus chinensis bunge in the embodiment, and directly spray the agent on 2-year-old male larva bodies of the fraxinus chinensis bunge, wherein the spraying amount is 2ul-5 ul/larva.
Comparative test
1.1 test methods
Exogenous juvenile hormone analogue is diluted into three different concentrations (25 mg.ml) of high, medium and low by using acetone aqueous solution with volume concentration of 1%-1、2.5mg•ml-1、1.25mg•ml-1) According to the nymph habit of the wax insect, three different methods of spraying, injecting and drying are selected to carry out exogenous juvenile hormone treatment in the 2-instar male larva stage (as shown in the table 1):
(1) spraying: diluting exogenous juvenile hormone with acetone water solution to different concentrations, and directly spraying onto insect body at a spraying amount of 3-3.2 ul/insect;
(2) and (3) injection: diluting exogenous juvenile hormone with acetone water solution to different concentrations, and injecting juvenile hormone solution into phloem of branch near the feeding point of Ceriporia alba at an injection amount of 3-3.2 ul/mouse;
(3) coating and drying: the exogenous juvenile hormone is diluted into different concentrations by acetone aqueous solution and directly smeared on the worm body by a brush pen. The coating amount is 3-3.2 ul/piece.
TABLE 1 test factors and levels
1.2 design of the experiment
Three-factor three-level test design is adopted, 1% acetone aqueous solution with the same volume is respectively used for replacing the medicament, controls (JH 0) are set according to different branches of the whole plant and the same plant, 3 plants are kept without any treatment and are used as blank Controls (CK), 10 times of repetition are respectively set for each concentration and method treatment, and the specific test design is shown in Table 2.
The JH1 is prepared by mixing exogenous juvenile hormone analog with 1% acetone aqueous solution, wherein the concentration of exogenous juvenile hormone analog in the preparation is 1.25 mg.ml-1。
The JH2 is prepared by mixing exogenous juvenile hormone analog with 1% acetone aqueous solution, wherein the concentration of exogenous juvenile hormone analog in the preparation is 2.5 mg.ml-1。
The JH3 is prepared by mixing exogenous juvenile hormone analog with 1% acetone aqueous solution, wherein the concentration of exogenous juvenile hormone analog is 25 mg.ml-1。
JH0 was prepared using an equal volume of 1% strength by volume aqueous acetone.
CK does not do anything.
The first four groups are respectively treated by spraying, drying and injecting three methods to treat male larva population of Ceriporiopsis chinensis 2 of Kunming area, and are compared with wax secretion (CK) of 2-instar male larva in nature state (as shown in figure 2). Under the spraying treatment, JH3 and JH2 have significant difference from CK, while JH0 and JH1 have insignificant difference from CK; under the coating dry treatment, the differences among JH1, JH2, JH3, JH0 and CK are not obvious; under the injection treatment, there was only a significant difference between JH3 and CK.
Then, the first four groups respectively adopt three methods of spraying, drying and injecting to treat 2-instar male larva populations of the cerambycis formosanus in the Anthriscus sylvestris area, and compare the 2-instar male larva populations with the individual wax excretion (CK) of the 2-instar male larvae in the natural state (as shown in figure 2). Under the spraying treatment, only a significant difference exists between JH2 and CK; under the condition of coating dry treatment, JH1 and JH2 have significant difference with CK; under the condition of spraying treatment, significant differences exist between JH0 and JH3 and CK, and the others are not significant.
In both Kunming and Sichuan Emei areas, JH2 showed significant differences from CK under spray treatment. The wax-up rates of individual wax insects in two regions under different treatments also showed differences in the increased yield under different concentrations of exogenous juvenile hormone analogue treatment (see table 3).
In Kunming areas, JH0, JH1 and JH3 show inhibition effects of different degrees on individual wax secretion amount of white wax worms under three treatment conditions of spraying, drying and injecting; JH2 shows promotion effect on individual wax secretion amount of the wax insect under three treatment conditions of spraying, drying and injecting, and the yield increase rate is 4.02% -41.50%, and JH2 shows the highest individual wax secretion amount increase rate of the wax insect under the spraying treatment. In the region of Szechwan Emei, JH0, JH1, JH2 and JH3 have promotion effect on individual wax secretion amount of white wax insect under three treatment conditions of spraying, drying and injection, and the yield is increased by 9.84-33.76%.
In the areas of Kunming and Sichuan Emei, JH2 has a promoting effect on the individual wax secretion amount of the white wax insects under the three treatment conditions of spraying, drying and injecting, and the yield increase rate of the individual wax secretion amount of the white wax insects in the areas of Kunming (41.50 mg/. holandra) and Emei (25.56 mg/. holandra) is higher than that under the treatment conditions of drying and injecting.
TABLE 3 increase of wax secretion yield of individual Ceriporiopsis chinensis treated at different concentrations in two regions
Exogenous juvenile hormone analogue is diluted into three different concentrations (25 mg.ml) of high, medium and low by using acetone aqueous solution with volume concentration of 1%-1、2.5mg•ml-1、1.25mg•ml-1) According to the nymph habit of the wax insect, three different methods of spraying, injecting and drying are selected to carry out exogenous juvenile hormone treatment in the 2-instar male larva stage. The test results show that medium and low concentration (2.5 mg.ml) is adopted-1And 1.25 mg.ml-1) The hormone analogue has obvious effect of promoting wax secretion of the wax insect individual, and is moderately and relatively superior to the juvenile hormone analogue with low concentration. For three different treatment modes, the wax secretion of the white wax insect is obviously promoted by spraying the juvenile hormone analogue with medium concentration. Therefore, the purpose of obviously increasing the wax can be achieved by spraying the juvenile hormone analogue with medium concentration (the average yield increase of 41.50% in the Kunming area: JH2 treatment and the average yield increase of 25.56% in the Emei area: JH2 treatment).
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (4)
1. A treatment method for increasing wax secretion amount of white wax insects is characterized by comprising the following steps: the pesticide is directly sprayed on the white wax moth body, the spraying amount is 2ul-5 ul/worm, and the pesticide is prepared from exogenous juvenile hormone analogue and concentrated in volumeMixing 1% -20% acetone aqueous solution; the concentration of exogenous juvenile hormone analogue is 2-10mg-1。
2. The treatment method of claim 1, wherein the exogenous juvenile hormone analog is: AltozarZR-512, MethopreneZR-515, KinoprenezR-777 or JH-286 farmoplant.
3. The treatment method according to claim 1, wherein the exogenous juvenile hormone analogue is present in a concentration of 2.5 mg.ml-1。
4. The processing method according to claim 1, characterized in that: the wax insect body is a 2-instar male larva.
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